Standard tube manufacturing device and method

Abstract

The invention discloses a standard tube manufacturing device and method. The device comprises a computer, a cable, a first air pipe, a first electromagnetic one-way valve, a high-pressure liquid injection pump, a second electromagnetic one-way valve, a first vacuum pump, a second air pipe, a liquid pipe, a filling liquid tank, a second vacuum pump and a motion control box. When the filling device is used for filling the standard tube, the operations of vacuumizing, liquid pumping and liquid injection can be continuously realized, the automation degree is high, the manufacturing efficiency of the standard tube can be improved, the stability of the filling quality of the standard tube can be ensured by automatic filling, and the standard tube with high quality and stability is obtained. Besides, the alcohol polymer is used as the filling liquid, the alcohol polymer is stable in physical and chemical performance, low in price, high in operability and capable of being stored for a long time at the normal temperature, the defects that expensive screening media (pop4, pop7 separation gel and the like) are used as the filling liquid, and the service life of the standard tube is short can be overcome, the consumable cost of the capillary electrophoresis technology is saved, and the cost of the capillary electrophoresis technology is reduced. The method has great application value.

Description

Technical Field

[0001] The invention relates to the technical field of biological detection equipment, and in particular to a standard tube manufacturing device and method. Background Art

[0002] The capillary array is composed of multiple elastic fused silica capillaries of equal length arranged regularly, and is composed of components such as the injection end and the injection end. A detection window with good light transmittance is provided near the injection end of the array, which is the electrophoresis channel for separating fluorescent labeled DNA fragments. The standard tube includes a capillary bundle composed of multiple elastic fused silica capillaries of equal length arranged regularly, and includes a injection end and a detection window. By filling liquid in each channel of the standard tube, the Raman peak generated after laser excitation at the detection window is obtained to evaluate the accuracy of the spatial positioning of each channel, providing a ruler for the optical path debugging of the array capillary electrophoresis instrument.

[0003] During the production and development stage, installation, and after-sales maintenance stage, array capillary electrophoresis instruments inevitably need to be debugged and calibrated for the optical path. The existing standard tube perfusion operation is usually manual, with a low degree of automation, and the quality of standard tube filling is poor, making it difficult to ensure the quality of standard tubes. In addition, currently, the entire set of capillary arrays is generally directly injected with glue for installation and adjustment. In this way, the stability of the glue injection state in the capillary channel is poor, and it has a great impact on the service life of the capillary array, increasing the cost of consumables for the array capillary electrophoresis instrument. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention aims to provide a standard tube manufacturing device and method.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] A standard tube manufacturing device, comprising a computer, a cable, an air pipe, an electromagnetic one-way valve, a high-pressure liquid injection pump, an electromagnetic one-way valve, a vacuum pump, a second air pipe, a liquid pipe, a filling liquid tank, a second vacuum pump and a motion control box;

[0007] A plunger is provided inside the high-pressure injection pump, and a driving motor is transmission-connected to the plunger and used to drive the plunger to reciprocate; the high-pressure injection pump includes at least port 1, port 2 and port 3, and the ports 1, 2 and 3 are interconnected inside the high-pressure injection pump and are all connected to the movement channel of the plunger; the port 1 is connected to the vacuum pump 2 through the air pipe 1, the port 2 is connected to the filling liquid tank through the electromagnetic one-way valve 1 and the liquid pipe, and the filling liquid tank is connected to the vacuum pump 1 through the air pipe 2; the port 3 is connected to the injection end of the standard tube through the electromagnetic one-way valve 2;

[0008] The computer is connected to the motion control box for communication, and the motion control box is connected to the electromagnetic one-way valve, the drive motor, the electromagnetic one-way valve, the vacuum pump, the stirring mechanism and the vacuum pump through cable control.

[0009] Furthermore, the standard tube manufacturing device also includes a liquid collecting bottle, which is used to collect liquid droplets discharged from the free end of the standard tube.

[0010] Furthermore, the standard tube manufacturing device also includes a stirring mechanism, and the stirring mechanism is arranged in the filling liquid tank.

[0011] Furthermore, the above-mentioned standard tube manufacturing device also includes a back plate, on which a fixing seat and a supporting sleeve are provided, the detection window of the standard tube is installed on the fixing seat, and the supporting sleeve is used to constrain and fix the quartz capillary bundle of the standard tube; the high-pressure injection pump is fixed to the back plate.

[0012] The present invention also provides a working method of the standard tube manufacturing device, the specific process is:

[0013] (1) injecting a filling liquid into a filling liquid tank; the filling liquid is an alcohol polymer;

[0014] (2) Locking control of electromagnetic check valve 1 and electromagnetic check valve 2 is performed through a motion control box;

[0015] (3) Controlling vacuum pump 1 and vacuum pump 2 through the motion control box to complete the vacuum operation of the interior of the high-pressure injection pump and the inner cavity of the filling liquid tank;

[0016] (4) The motion control box unlocks the electromagnetic check valve 1 and controls the drive motor to start. The drive motor drives the plunger to move upward from the original bottom zero position. The filling liquid in the filling liquid tank flows upward into the interior of the high-pressure injection pump through the liquid pipe. After the interior of the high-pressure injection pump is filled with the filling liquid, the motion control box locks the electromagnetic check valve 1 again.

[0017] (5) The motion control box unlocks the electromagnetic one-way valve 2 and starts the drive motor again. The drive motor drives the plunger to move downward from the internal top terminal position of the high-pressure injection pump. At the same time, the filling liquid inside the high-pressure injection pump enters the quartz capillary bundle of the standard tube through the injection end of the standard tube. At this time, observe from the free end of the standard tube whether there are droplets dripping from each fused quartz capillary. This completes the filling operation of the standard tube.

[0018] Furthermore, in step (3), when the inner cavity of the filling liquid tank is vacuumed, the stirring mechanism in the filling liquid tank is used to stir and remove bubbles, thereby effectively removing bubbles from the alcohol polymer.

[0019] Furthermore, the working method further comprises step (6): after the filling operation is completed, the clustering tube is inserted into the free end of the standard tube, and the fused silica capillary at the free end is cut using a ceramic blade so that the length of the free end after the clustering tube is inserted is consistent with the length of the injection end, and finally the free end and the injection end are cured and sealed using a sealing sleeve and a two-component epoxy glue to ensure that the filling liquid in the standard tube does not flow out.

[0020] The beneficial effects of the present invention are:

[0021] 1. The present invention is used to perform the perfusion operation of the standard tube, and the operations of vacuuming, pumping and injecting can be realized continuously. Not only is the degree of automation high, which can improve the production efficiency of the standard tube, but the automated perfusion can also ensure the stability of the perfusion quality of the standard tube, thereby obtaining a high-quality and stable standard tube.

[0022] 2. The present invention uses alcohol polymers as filling liquids. Alcohol polymers have stable physical and chemical properties, low prices, strong operability, and can be stored for a long time at room temperature. They can solve the drawbacks of using expensive sieving media (POP4, POP7 separation gels, etc.) as filling liquids and short service life of standard tubes, saving the consumables cost of capillary electrophoresis technology and having great application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the structure of the standard tube manufacturing device in Example 1 of the present invention;

[0024] Figure 2 This is a schematic diagram of the standard tube after sealing in Example 2 of the present invention;

[0025] Figure 3 This is a schematic diagram of the process of performing spatial calibration using the manufactured standard tube in Example 3 of the present invention;

[0026] Figure 4 This is a schematic diagram of the results of spatial correction using the manufactured standard tube in Example 3 of the present invention. DETAILED DESCRIPTION

[0027] The present invention will be further described below in conjunction with the accompanying drawings. It should be noted that this embodiment is based on the technical solution and provides a detailed implementation method and specific operation process, but the protection scope of the present invention is not limited to this embodiment.

[0028] Example 1

[0029] This embodiment provides a standard tube manufacturing device, which is used to implement high-pressure liquid injection filling of each channel of the standard tube, and finally achieve packaging and molding of the standard tube.

[0030] like Figure 1As shown, the standard tube includes a quartz capillary bundle 12 formed by a plurality of fused silica capillaries arranged in a regular pattern, a detection window 10 is provided on the quartz capillary bundle 12, one end of the quartz capillary bundle 12 is a glue injection end, and the other end is a free end. Among them, the fused silica capillary corresponding to the detection window needs to be de-coated and opened to provide a laser excitation detection position. This embodiment takes a 24-channel standard tube as an example. The standard tube is packaged by 24 windowed capillaries, wherein the glue injection end surface is sealed with epoxy glue and then cut and ground, and at the same time, each channel needs to be ensured to be unobstructed.

[0031] The standard tube manufacturing device of this embodiment is as follows Figure 1 As shown, it includes a computer 1, a cable 2, an air pipe 3, an electromagnetic one-way valve 4, a high-pressure injection pump 7, an electromagnetic one-way valve 8, a fixing seat 11, a support sleeve 13, a back plate 14, a liquid collecting bottle 16, a vacuum pump 17, an air pipe 2 18, a stirring mechanism 19, a liquid pipe 20, a filling liquid tank 21, a vacuum pump 2 22 and a motion control box 23.

[0032] A plunger 5 is provided inside the high-pressure injection pump 7, and a driving motor 6 is transmission-connected to the plunger 5 and used to drive the plunger 5 to reciprocate; the high-pressure injection pump 7 includes at least port one, port two and port three, and the port one, port two and port three are interconnected inside the high-pressure injection pump 7 and are all connected to the movement channel of the plunger 5; the port one is connected to the vacuum pump 22 through the air pipe 13, and the port two is connected to the filling liquid tank 21 through the electromagnetic one-way valve 14 and the liquid pipe 20, and the filling liquid tank 21 is connected to the vacuum pump 17 through the air pipe 18; the port three is connected to the injection end 9 of the standard tube through the electromagnetic one-way valve 28, and the liquid collecting bottle 16 is used to collect the droplets 15 discharged from the free end of the standard tube; the stirring mechanism 19 is arranged in the filling liquid tank 21.

[0033] The computer 1 is communicatively connected to the motion control box 23, and the motion control box 23 is controlled and connected to the electromagnetic one-way valve 1 4, the drive motor 6, the electromagnetic one-way valve 2 8, the vacuum pump 1 17, the stirring mechanism 19 and the vacuum pump 2 22 through the cable 2.

[0034] The control logic and control parameters of the motion control box are pre-set by the computer, and the motion control box controls the drive motor 6, vacuum pump 22, vacuum pump 17, stirring mechanism 19, electromagnetic check valve 14, and electromagnetic check valve 28 accordingly to realize motion control such as liquid injection and vacuum pumping, thereby completing Figure 1 The operations shown are vacuuming operation a, vacuuming operation b, stirring and bubbling operation c, liquid aspiration operation d, liquid aspiration operation e, liquid injection operation f, and liquid injection operation g.

[0035] In this embodiment, the standard tube manufacturing device also includes a back plate 14, on which a fixing seat 11 and a support sleeve 13 are provided. The detection window of the standard tube is installed on the fixing seat 11, and the support sleeve 13 is used to constrain and fix the quartz capillary bundle 12 of the standard tube; the high-pressure injection pump 7 is fixed to the back plate 14.

[0036] Example 2

[0037] This embodiment provides a working method of a standard tube manufacturing device, and the specific process is as follows:

[0038] (1) Inject the filling liquid into the filling liquid tank 21. In this embodiment, the filling liquid is an alcohol polymer. Alcohol polymers have stable chemical properties, obvious Raman peaks, can be stored for a long time at room temperature, are inexpensive, and are easy to prepare. The corresponding concentration ratio can be prepared according to the Raman peak height requirements. The alcohol polymer includes one or more of polyvinyl alcohol, polyethylene glycol, polypropylene alcohol, polyvinyl butyral, and alcohol-modified polymers. The preparation method includes but is not limited to free radical polymerization, ring-opening polymerization, hydrolysis reaction, acetalization, enzymatic synthesis, and copolymerization.

[0039] (2) The electromagnetic one-way valve 1 4 and the electromagnetic one-way valve 2 8 are locked and controlled through the motion control box 23.

[0040] (3) The vacuum pump 17 and the vacuum pump 22 are controlled by the motion control box 23 to perform vacuum operation a and vacuum operation b respectively, thereby completing the vacuum operation of the interior of the high-pressure injection pump 7 and the inner cavity of the filling liquid tank 21. When the vacuum operation is performed on the inner cavity of the filling liquid tank 21, the stirring and bubble removal operation c is achieved by stirring the stirring mechanism 19, thereby effectively emptying the bubbles in the alcohol polymer.

[0041] (4) The motion control box 23 unlocks the electromagnetic one-way valve 4 and controls the drive motor 6 to start. The drive motor 6 drives the plunger 5 to move upward from the original bottom zero position to complete the liquid suction operation e. The filling liquid in the filling liquid tank 21 enters the interior of the high-pressure injection pump 7 upward through the liquid pipe 20 to complete the liquid suction operation d. After the interior of the high-pressure injection pump 7 is filled with the filling liquid, the motion control box 23 locks the electromagnetic one-way valve 4 again;

[0042] (5) The motion control box 23 unlocks the electromagnetic check valve 28 and starts the drive motor 6 again. The drive motor 6 drives the plunger 5 to move downward from the internal top terminal position of the high-pressure injection pump 7 to complete the injection operation f. At the same time, the filling liquid inside the high-pressure injection pump 7 enters the quartz capillary bundle of the standard tube through the injection end of the standard tube, thereby completing the injection operation g. At this time, it can be observed from the free end of the standard tube whether there are droplets 15 dripping from each fused silica capillary, and the dripping droplets 15 are collected by the liquid collection bottle 16;

[0043] This completes the filling operation of the standard tube filling liquid (alcohol polymer).

[0044] (6) After the filling operation is completed, the cluster tube 24 is inserted into the free end of the standard tube, and the fused silica capillary at the free end is cut with a ceramic blade so that the length of the free end after the cluster tube 24 is inserted is consistent with the length of the injection end. Finally, the free end and the injection end are sealed with a sealing sleeve 25 and a two-component epoxy adhesive to ensure that the filling liquid in the standard tube does not flow out. Figure 2 shown.

[0045] Example 3

[0046] Based on Example 1 and Example 2, the standard tube used in the array capillary electrophoresis instrument was manufactured. In this embodiment, the manufactured standard tube was installed in the array capillary electrophoresis instrument to perform a spatial correction performance test to verify its reliability as a scale for optical path debugging and calibration. The spatial correction process is as follows: Figure 3 The spatial correction result spectrum is shown as Figure 4 shown.

[0047] Figure 4 The results shown show a clear Raman spectrum, with sharp and continuous peaks in each channel, consistent peak height, peak spacing of 15 to 16 pixels, and half-peak width of 2.7 to 3.1. The spatial correction performance is excellent and can be used as a scale for optical path debugging and calibration. Moreover, due to the stable physical and chemical properties of alcohol polymers, low price, strong operability, and long-term storage at room temperature, it solves the problem of using expensive sieving media as internal filling liquids and short service life of standard tubes, saving the consumables cost of capillary electrophoresis technology, and has great application value.

[0048] For those skilled in the art, various corresponding changes and modifications can be made according to the above technical solutions and concepts, and all of these changes and modifications should be included in the protection scope of the claims of the present invention.

Claims

1. A standard tube manufacturing device, characterized in that: It includes a computer, a cable, an air pipe, an electromagnetic one-way valve, a high-pressure liquid injection pump, an electromagnetic one-way valve, a vacuum pump, an air pipe, a liquid pipe, a filling liquid tank, a vacuum pump and a motion control box; A plunger is provided inside the high-pressure injection pump, and a driving motor is transmission-connected to the plunger and used to drive the plunger to reciprocate; the high-pressure injection pump includes at least port 1, port 2 and port 3, and the ports 1, 2 and 3 are interconnected inside the high-pressure injection pump and are all connected to the movement channel of the plunger; the port 1 is connected to the vacuum pump 2 through the air pipe 1, the port 2 is connected to the filling liquid tank through the electromagnetic one-way valve 1 and the liquid pipe, and the filling liquid tank is connected to the vacuum pump 1 through the air pipe 2; the port 3 is connected to the injection end of the standard tube through the electromagnetic one-way valve 2; The computer is connected to the motion control box for communication, and the motion control box is connected to the electromagnetic one-way valve, the drive motor, the electromagnetic one-way valve, the vacuum pump, the stirring mechanism and the vacuum pump through cable control.

2. The standard tube manufacturing device according to claim 1, characterized in that: Also included is a liquid collecting bottle, which is used to collect liquid drops discharged from the free end of the standard tube.

3. The standard tube manufacturing device according to claim 1, characterized in that: It also includes a stirring mechanism, which is arranged in the filling liquid tank.

4. The standard tube manufacturing device according to claim 1, characterized in that: It also includes a back plate, which is provided with a fixing seat and a supporting sleeve. The detection window of the standard tube is installed on the fixing seat, and the supporting sleeve is used to constrain and fix the quartz capillary bundle of the standard tube; the high-pressure injection pump is fixed to the back plate.

5. A working method of the standard tube manufacturing device according to any one of claims 1 to 4, characterized in that: The specific process is: (1) injecting a filling liquid into a filling liquid tank; the filling liquid is an alcohol polymer; (2) Locking control of electromagnetic check valve 1 and electromagnetic check valve 2 is performed through a motion control box; (3) Controlling vacuum pump 1 and vacuum pump 2 through the motion control box to complete the vacuum operation of the interior of the high-pressure injection pump and the inner cavity of the filling liquid tank; (4) The motion control box unlocks the electromagnetic check valve 1 and controls the drive motor to start. The drive motor drives the plunger to move upward from the original bottom zero position. The filling liquid in the filling liquid tank flows upward into the interior of the high-pressure injection pump through the liquid pipe. After the interior of the high-pressure injection pump is filled with the filling liquid, the motion control box locks the electromagnetic check valve 1 again. (5) The motion control box unlocks the electromagnetic one-way valve 2 and starts the drive motor again. The drive motor drives the plunger to move downward from the internal top terminal position of the high-pressure injection pump. At the same time, the filling liquid inside the high-pressure injection pump enters the quartz capillary bundle of the standard tube through the injection end of the standard tube. At this time, observe from the free end of the standard tube whether there are droplets dripping from each fused quartz capillary. This completes the filling operation of the standard tube.

6. The working method according to claim 5, characterized in that: In step (3), when the inner cavity of the filling liquid tank is vacuumed, the stirring mechanism in the filling liquid tank is used to stir and remove bubbles, thereby effectively removing bubbles from the alcohol polymer.

7. The working method according to claim 5, characterized in that: The method further comprises step (6): after the filling operation is completed, the cluster tube is inserted into the free end of the standard tube, and the fused silica capillary at the free end is cut with a ceramic blade so that the length of the free end after the cluster tube is inserted is consistent with the length of the injection end, and finally the free end and the injection end are cured and sealed with a sealing sleeve and a two-component epoxy glue to ensure that the filling liquid in the standard tube does not flow out.

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