A partitioned culture device for mouse cells

Abstract

The present invention belongs to the field of cell culture technology, and in particular to a partitioned culture device for mouse cells. In view of the problem that errors are prone to occur when dripping nutrient solution into multiple culture dishes in the prior art, the following scheme is proposed, including a base with an opening downward and an overall barrel-shaped structure, a plurality of rectangular sliding holes distributed centripetally and in an elongated structure are opened at the top of the base near the circumferential edge, each of the rectangular sliding holes is slidably connected with a folding push rod, and a horizontal C-shaped clip with an outward opening is reserved at the top of the folding push rod; a culture dish is clamped in each of the C-shaped clips; when the present invention is used to add nutrient solution to each culture dish, it is only necessary to ensure that the liquid level in the glass capillary drops to a consistent level each time; after the liquid level drops to the lowest, the piston plate is pushed downward to raise the liquid level in the glass capillary again; and after each filling, one station is rotated again to avoid repeated filling of the culture dish.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a partitioned culture device for mouse cells. Background Art

[0002] Cell culture is a highly precise experimental technique that is widely used in biology, medical research, biopharmaceuticals, etc. In order to obtain healthy and stable cell lines and avoid problems during the culture process, special attention should be paid to the observation of the environment during the cell culture process, such as ensuring constant temperature and humidity, as well as the supply of nutrient solution.

[0003] After searching, it was found that nowadays, in order to ensure that each culture dish is filled with the same amount of nutrient solution when adding culture medium, usually an equal dose of nutrient solution is sucked out each time with a dropper, and then filling is carried out. This filling method is not only time-consuming and laborious, but also prone to mistakes due to frequent operations. Moreover, it is easy to confuse the culture dishes that have been filled and the culture dishes that have not been filled during multiple fillings, resulting in repeated dripping. Therefore, we designed a new filling device that can fill multiple culture dishes with the same dose without repeated dripping, so as to reduce the complexity of adding nutrient solution during culture. Summary of the invention

[0004] In view of the technical problem in the prior art that errors are prone to occur when dripping nutrient solution into multiple culture dishes, the present invention adopts the following technical solution:

[0005] A partition culture device for mouse cells, comprising a base with an opening downward and an overall barrel-shaped structure, a plurality of rectangular sliding holes distributed centripetally and in a long strip structure are opened at the top of the base near the circumferential edge, each of the rectangular sliding holes is slidably connected with a folding push rod, and a horizontal C-shaped clip with an opening facing outward is reserved at the top of the folding push rod; a culture dish is clamped in each C-shaped clip; an anti-slip bearing is embedded in the middle of the base, and a supporting rotating tube is rotatably connected inside the anti-slip bearing; and a rotating top cover is fixed to the top of the supporting rotating tube, a mounting hole is opened at the rotating top cover near the circumferential edge, a rubber ring is embedded in the mounting hole, and a liquid outlet pipe is inserted in the rubber ring, a valve is fixed to the top of the liquid outlet pipe, and a liquid separation leak pot is fixed to the top of the valve, the liquid separation leak pot comprises a vertical barrel and an adjusting inclined tube obliquely inserted and connected with the vertical barrel, a glass capillary coaxial with the liquid outlet pipe is inserted at the top of the vertical barrel, and a piston plate is slidably connected in the adjusting inclined tube.

[0006] A further configuration of the present invention is that an internally threaded fixing ring is screwed on the circumferential outer wall of the top opening of the adjusting inclined tube; centripetally distributed spoke rods are fixed on the circumferential inner wall of the internally threaded fixing ring, and the same hexagonal nut is fixed in the middle of multiple spoke rods, and an adjusting advance screw is screwed in the hexagonal nut; a pan head is fixed on the end of the adjusting advance screw close to the piston plate, and a limiting ring for clamping the pan head is fixed on the surface of the piston plate.

[0007] A further configuration of the present invention is that the top tube opening of the adjusting inclined tube is higher than the top height of the vertical tube, so that when adding nutrient solution, it can be ensured that the vertical tube of the liquid separation leaky pot can be directly filled and then quickly overflow into the glass capillary tube. A drainage rod is inserted and fixed to the inner wall of the bottom end of the liquid outlet tube, which can drain the nutrient solution to the bottom of the culture dish as much as possible to prevent impact and splashing caused by excessive flow.

[0008] A further configuration of the present invention is that a scale plate is fixed to the circumferential outer wall of the glass capillary tube, and the glass capillary tube can be replaced with different diameters according to the needs of the filling amount, and there is also a matching scale plate corresponding thereto.

[0009] A further configuration of the present invention is that a support frame is fixed on the upper surface of the rotating top cover near the bottom of the adjusting inclined tube, and a centripetal through hole is opened on the side of the support frame away from the supporting rotating tube, and a sliding bearing is clamped in the through hole, and a transmission rod is rotatably connected in the sliding bearing; a protruding adjusting rod is reserved on the side of the valve near the support frame, and one end of the transmission rod near the adjusting rod is connected to the end of the adjusting rod through a coupling; a driven pinion is fixed to the other end of the transmission rod; a toggle hand wheel meshing with the driven pinion is arranged on the side of the support frame near the top of the driven pinion; and the diameter of the toggle hand wheel is larger than the diameter of the driven pinion; the valve opening can be opened as small as possible by the toggle hand wheel.

[0010] A further configuration of the present invention is that the diameter of the rotating top cover is equal to the diameter of the top disk of the base, and an annular sterile protective frame is fixed on the upper surface of the top disk of the base near the circumferential edge, and the annular sterile protective frame is made of transparent material, and a plurality of outlets are opened on the circumferential outer wall of the annular sterile protective frame near the bottom end, and the width and length of the outlets are adapted to the outer dimensions of the culture dish and the C-shaped clamp; a number is provided above each of the outlets; through such a configuration, it can be clearly observed whether each droplet is successful, thereby ensuring the success of each droplet.

[0011] A further configuration of the present invention is that the outer wall of the annular sterile protective frame is hinged with a side cover on the side near the outlet, and a reset torsion spring is arranged inside the hinge of the side cover; when the culture dish is pushed out for sampling, the side cover can be opened by slowly pushing it out, and the side cover can be automatically closed after being pushed in.

[0012] A further arrangement of the present invention is that a spring baffle is fixed between the end of the rectangular sliding hole and the anti-slip bearing on the top inner wall of the base, and a main tension spring is fixed between the spring baffle and the vertical rod of the folding push rod of the work station; an arc-shaped groove is opened on the lower surface of the end of the folding push rod away from the C-shaped clip; a pushing mechanism is arranged in the supporting rotating tube, and the pushing mechanism includes two angle steel slide rails fixed to the two openings of the supporting rotating tube, which are opposite to each other and parallel to each other, and the same T-shaped sliding rod is slidably connected between the two angle steel slide rails, the center line of the T-shaped sliding rod intersects with the axial line of the supporting rotating tube, and an L-shaped stop block is fixed on the end of the T-shaped sliding rod close to the liquid separating leak pot, and an arc-shaped stop block is arranged on the end of the L-shaped stop block away from the T-shaped sliding rod The slot can be engaged with an arc-shaped stop block; and two return springs are fixed on both sides of the arc-shaped stop block; one end of the two angle steel slide rails close to the L-shaped stop block is fixed with a hook for fixing the two return springs; the lower surface of the other end of the angle steel slide rail is fixed with a limiting convex column, and the side of the T-shaped slide bar is plugged with a limiting strip near the bottom end; through the setting of the limiting strip and the two return springs, it is ensured that the L-shaped stop block at the end of the T-shaped slide bar can be withdrawn to the original position after each push, and will not be blocked by other folding push rods when rotating; at this time, when it is necessary to push out the culture dish clamped by the top end of the folding push rod, it is only necessary to rotate the L-shaped stop block to align with the arc-shaped slot, and then pull the T-shaped slide bar to the side of the liquid separation leak pot.

[0013] A further arrangement of the present invention is that a roller groove is opened at the bottom end of the supporting rotating tube between two angle steel slide rails, and a fixed pulley 2 is arranged in the roller groove, a perforated anti-falling cover is fixed to the top end of the supporting rotating tube, and a pull rope is fixed to the upper surface of the end of the T-shaped slide bar away from the L-shaped stop block, and the pull rope passes around the fixed pulley 2 and passes through the perforated anti-falling cover to be fixed with a pull ring; a centripetally distributed Y-shaped composite roller frame is fixed on the side of the upper surface of the rotating top cover away from the liquid separating leak pot, and a fixed pulley 1 is arranged on the end of the Y-shaped composite roller frame close to the supporting rotating tube; a plurality of limit pins for socketing the pull ring are fixed to the upper surface of the other end of the Y-shaped composite roller frame; each of the limit pins is at a different distance from the center of the supporting rotating tube.

[0014] A further configuration of the present invention is that a second magnet is embedded on the upper surface of one end of the L-shaped stop block away from the T-shaped slide bar, and a first magnet that attracts the second magnet is disposed at the bottom of the arc-shaped slot at the bottom end of the folded push rod.

[0015] The beneficial effects of the present invention are:

[0016] 1. By setting a liquid-dispensing leak pot with a glass capillary above the culture dish, when adding nutrient solution to each culture dish, it is only necessary to ensure that the liquid level in the glass capillary drops to the same extent each time; after the liquid level drops to the lowest, the piston plate can be pushed down to raise the liquid level in the glass capillary again; and after each filling, the workstation is rotated one more time to avoid repeated filling of the culture dish.

[0017] 2. By providing the internal thread fixing ring and the hexagonal nut in the middle, when the nutrient solution needs to be added to the interior of the liquid separation leak pot before use, the internal thread fixing ring can be removed by simply reversely twisting the entire ring to expose the nozzle of the adjustable inclined tube, which is convenient for adding the nutrient solution.

[0018] 3. When you need to push the culture dish out for sampling, just pull the pull ring upwards. At this time, the pull rope will pass around one end of the fixed pulley 2 to generate pulling force on the T-shaped slide bar. After it is in place, hang the pull ring on the limit nail. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the overall structure of a partitioned culture device for mouse cells proposed by the present invention;

[0020] Figure 2 This is a schematic diagram of a three-dimensional structure of a partitioned culture device for mouse cells proposed by the present invention when viewed from above;

[0021] Figure 3 A top view of a partitioned culture device for mouse cells proposed by the present invention;

[0022] Figure 4 A partitioned culture device for mouse cells proposed by the present invention Figure 3 Schematic diagram of the cross-sectional structure along line AA;

[0023] Figure 5 This is a schematic diagram of the overall structure of a partitioned culture device for mouse cells proposed by the present invention when sampling;

[0024] Figure 6 A schematic diagram of the bottom structure of a partitioned culture device for mouse cells proposed by the present invention during sampling;

[0025] Figure 7 A schematic diagram of the exploded structure of a liquid separation leak pot of a mouse cell partition culture device proposed by the present invention;

[0026] Figure 8 This is a schematic diagram of the three-dimensional structure of a pushing mechanism in a mouse cell partitioning culture device proposed in the present invention when it is turned to a target position.

[0027] In the figure: 1, base; 101, rectangular slide hole; 2, annular aseptic protection frame; 201, outlet; 3, rotating top cover; 4, limit pin; 5, Y-shaped composite roller frame; 6, fixed pulley 1; 7, pull ring; 8, scale plate; 9, pull rope; 10, glass capillary; 11, liquid separation clepsydra; 1101, adjusting inclined tube; 1102, valve; 1103, drainage rod; 12, adjusting push screw; 13, internal thread fixing ring; 14, support frame; 15 , toggle hand wheel; 16, driven pinion; 17, side cover; 18, anti-slip bearing; 19, support rotating tube; 20, angle steel slide rail; 21, main tension spring one; 22, T-shaped slide bar; 2201, L-shaped block; 2202, return spring two; 23, folding push rod; 2301, arc-shaped slot; 2302, C-shaped clamp; 24, magnet one; 25, limiting boss; 26, culture dish; 27, piston plate; 28, fixed pulley two; 29, magnet two. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0029] In this embodiment, refer to Figure 1-8A partitioning culture device for mouse cells comprises a base 1 with an opening downward and an overall barrel-shaped structure, a plurality of rectangular sliding holes 101 with centripetal distribution and in a long strip structure are opened near the circumferential edge at the top of the base 1, each rectangular sliding hole 101 is slidably connected with a folding push rod 23, and a horizontal C-shaped clip 2302 with an opening facing outward is reserved at the top of the folding push rod 23; the C-shaped clip 2302 has a certain toughness to ensure that culture dishes 26 of different diameters can be clamped; a culture dish 26 is clamped in each C-shaped clip 2302; an anti-channeling bearing 18 is embedded in the middle of the base 1, and a supporting rotating tube 19 is rotatably connected to the inside of the anti-channeling bearing 18; and a rotating top cover 3 is fixed to the top of the supporting rotating tube 19, and a mounting hole is opened near the circumferential edge of the rotating top cover 3, and the mounting hole is located just above the culture dish 26, and a A rubber ring is provided, and a liquid outlet pipe is inserted in the rubber ring, a valve 1102 is fixed on the top of the liquid outlet pipe, a liquid separating clepsydra 11 made of glass is fixed on the top of the valve 1102, the liquid separating clepsydra 11 comprises a vertical barrel and an adjusting inclined tube 1101 which is obliquely inserted and connected with the vertical barrel, a glass capillary 10 which is coaxial with the liquid outlet pipe is inserted on the top of the vertical barrel, and a piston plate 27 is slidably connected in the adjusting inclined tube 1101; by arranging the liquid separating clepsydra 11 with the glass capillary 10 above the culture dish 26, when filling nutrient solution to each culture dish 26, it is only necessary to ensure that the liquid level in the glass capillary 10 drops to the same extent each time; after the liquid level drops to the minimum, the piston plate 27 is pushed downward to raise the liquid level in the glass capillary 10 again; and after each filling, the piston plate 27 is rotated by one station to avoid repeated filling of the culture dish 26.

[0030] In the present invention, an internally threaded fixing ring 13 is screwed on the circumferential outer wall of the top opening of the adjusting inclined tube 1101; centripetally distributed spoke rods are fixed on the circumferential inner wall of the internally threaded fixing ring 13, and the same hexagonal nut is fixed in the middle of multiple spoke rods, and the adjusting advancing screw 12 is screwed in the hexagonal nut; a pan head is fixed at the end of the adjusting advancing screw 12 close to the piston plate 27, and a limiting ring for clamping the pan head is fixed on the surface of the piston plate 27; through the provision of the internally threaded fixing ring 13 and the hexagonal nut in the middle, when it is necessary to add nutrient solution to the interior of the liquid separating leak pot 11 before use, the internally threaded fixing ring 13 only needs to be reversely screwed and removed as a whole to expose the pipe mouth of the adjusting inclined tube 1101, so as to facilitate the filling of nutrient solution.

[0031] Reference Figure 4-Figure 5 , adjust the top tube opening of the inclined tube 1101 to be higher than the top height of the vertical tube, so that when adding nutrient solution, it can be ensured that the vertical tube of the liquid separation leak pot 11 can be directly filled and then quickly overflow into the glass tube 10, and a drainage rod 1103 is fixed to the inner wall of the bottom end of the liquid outlet tube, which can drain the nutrient solution to the bottom of the culture dish 26 as much as possible to prevent impact and splashing caused by excessive flow.

[0032] Reference Figure 5 A scale plate 8 is fixed to the circumferential outer wall of the glass capillary 10, and the glass capillary 10 can be replaced with different diameters according to the required filling amount, and there is also a matching scale plate 8 corresponding to it; glass capillary 10 with different inner diameters can be selected according to the required filling amount and error.

[0033] Reference Figure 5 and Figure 7 A support frame 14 is fixed on the upper surface of the rotating top cover 3 near the bottom of the adjusting inclined tube 1101, and a centripetal through hole is opened on the side of the support frame 14 away from the supporting rotating tube 19, and a sliding bearing is clamped in the through hole, and a transmission rod is rotatably connected in the sliding bearing; a protruding adjusting rod is reserved on the side of the valve 1102 near the support frame 14, and the end of the transmission rod near the adjusting rod is connected to the end of the adjusting rod through a coupling; a driven pinion 16 is fixed to the other end of the transmission rod; a toggle hand wheel 15 meshing with the driven pinion 16 is arranged on the side of the support frame 14 near the top of the driven pinion 16; and the diameter of the toggle hand wheel 15 is larger than the diameter of the driven pinion 16; the toggle hand wheel 15 can be set to open the valve 1102 as small as possible.

[0034] Reference Figure 6 The diameter of the rotating top cover 3 is equal to the diameter of the top disk of the base 1, and an annular sterile protection frame 2 is fixed on the upper surface of the top disk of the base 1 near the circumferential edge, and the annular sterile protection frame 2 is made of transparent material, and a plurality of outlets 201 are opened near the bottom of the circumferential outer wall of the annular sterile protection frame 2, and the width and length of the outlet 201 are adapted to the outer dimensions of the culture dish 26 and the C-shaped clip 2302; a number is set above each outlet 201; through such a setting, it can be clearly observed whether each drop is successful, ensuring the success of each drop.

[0035] Reference Figure 6 The outer wall of the annular sterile protection frame 2 is hinged with a side cover 17 on the side near the outlet 201, and a reset torsion spring is arranged inside the hinge of the side cover 17; when the culture dish 26 is pushed out for sampling, the side cover 17 can be opened by slowly pushing it out, and the side cover 17 can be automatically closed after being pushed in.

[0036] Reference Figure 2 , Figure 4 , Figure 6 and Figure 8A spring baffle is fixed between the end of the rectangular sliding hole 101 and the anti-slip bearing 18 on the top inner wall of the base 1, and a main tension spring 21 is fixed between the spring baffle and the vertical rod of the folding push rod 23 of the work station; an arc-shaped groove 2301 is opened on the lower surface of the end of the folding push rod 23 away from the C-shaped clip 2302; a pushing mechanism is arranged in the support rotating tube 19, and the pushing mechanism includes two angle steel slide rails 20 with openings opposite to each other and parallel to each other fixed on the support rotating tube 19, and the same T-shaped slide bar 22 is slidably connected between the two angle steel slide rails 20, the center line of the T-shaped slide bar 22 intersects with the axial line of the support rotating tube 19, and an L-shaped block 2201 is fixed on the end of the T-shaped slide bar 22 close to the liquid separation leak pot 11, and the end of the L-shaped block 2201 away from the T-shaped slide bar 22 is provided with a snap-fitting arc-shaped groove 2301 Arc-shaped resistance block; and both sides of the arc-shaped resistance block are fixed with return spring 2202; one end of the two angle steel slide rails 20 close to the L-shaped resistance block 2201 is fixed with a hook for fixing the return spring 2202; the lower surface of the other end of the angle steel slide rail 20 is fixed with a limiting protrusion 25, and the side of the T-shaped slide bar 22 is connected with a limiting strip near the bottom; through the setting of the limiting strip and the return spring 2202, it is ensured that the L-shaped resistance block 2201 at the end of the T-shaped slide bar 22 can be withdrawn to the original position after each push, and will not be blocked by other folding push rods 23 when rotating; at this time, when it is necessary to push out the culture dish 26 clamped at the top of the folding push rod 23, it is only necessary to rotate the L-shaped resistance block 2201 to align with the arc-shaped slot 2301, and then pull the T-shaped slide bar 22 to the side of the liquid separation leak pot 11.

[0037] Reference Figure 4 and Figure 8 The bottom end of the supporting rotating tube 19 is located between the two angle steel slide rails 20 and has a roller groove, and a second fixed pulley 28 is arranged in the roller groove, and a hole-protecting cover with a hole is fixed to the top of the supporting rotating tube 19, and a pull rope 9 is fixed to the upper surface of the end of the T-shaped slide bar 22 away from the L-shaped stop block 2201, and the pull rope 9 passes around the second fixed pulley 28 and passes through the hole-protecting cover with a pull ring 7; a centripetally distributed Y-shaped composite roller frame 5 is fixed to the side of the upper surface of the rotating top cover 3 away from the liquid separation clepsydra 11, and the Y-shaped composite roller frame 5 A fixed pulley 6 is provided near one end of the supporting rotating tube 19; a plurality of limit pins 4 for sleeved on the pull ring 7 are fixed to the upper surface of the other end of the Y-shaped composite roller frame 5; when it is necessary to push the culture dish 26 out for sampling, it is only necessary to pull the pull ring 7 upwards, at which time the pull rope 9 can pass around one end of the fixed pulley 28 to generate a pulling force on the T-shaped slide bar 22, and then the pull ring 7 can be hung on the limit pin 4 after it is in place, and each limit pin 4 is at a different distance from the center of the supporting rotating tube 19, which can prevent the pull rope 9 from becoming loose after long-term use.

[0038] Reference Figure 2 and Figure 8A second magnet 29 is embedded on the upper surface of one end of the L-shaped stop block 2201 away from the T-shaped slide bar 22, and a magnet 24 that attracts the second magnet 29 is arranged at the bottom of the arc-shaped slot 2301 at the bottom end of the folded push rod 23; when the L-shaped stop block 2201 is rotated to the docking position, suction is generated to facilitate pushing at the optimal position.

[0039] Working principle: Before using the device, first reversely screw the internal thread fixing ring 13 until it and the adjusting push screw 12 connected thereto and the piston plate 27 slidably connected to the adjusting inclined tube 1101 are all pulled out, and then add culture medium into the liquid separating leaky pot 11 from the nozzle of the adjusting inclined tube 1101 until the adjusting inclined tube 1101 is filled, at which time part of the nutrient solution will overflow into the bottom position of the glass capillary 10; then insert the piston plate 27 and fix the internal thread fixing ring 13; when adding nutrient solution to each culture dish 26, just make sure that each time the glass capillary 10 is filled with nutrient solution. The liquid level in the tube 10 only needs to drop at the same rate; after the liquid level drops to the lowest, the piston plate 27 can be pushed downward to raise the liquid level in the glass tube 10 again; and after each filling, it is rotated by one station to avoid repeated filling of the culture dish 26; when it is necessary to push out the culture dish 26 clamped at the top of the folding push rod 23, it is only necessary to rotate the L-shaped stop block 2201 to align with the arc-shaped slot 2301; then pull the pull ring 7 upward, and at this time, the pull rope 9 can pass around one end of the fixed pulley 28 to generate a pulling force on the T-shaped slide bar 22, and after it is in place, the pull ring 7 can be hung on the limit nail 4.

[0040] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A partitioned culture device for mouse cells, comprising a base (1) with an opening downward and an overall barrel-shaped structure, wherein a plurality of rectangular sliding holes (101) distributed centripetally and in a long strip structure are formed near the circumferential edge of the top of the base (1), characterized in that: A folding push rod (23) is slidably connected in each of the rectangular sliding holes (101), and a horizontal C-shaped clip (2302) with an opening facing outward is reserved at the top of the folding push rod (23); a culture dish (26) is clamped in each of the C-shaped clips (2302); an anti-slip bearing (18) is embedded in the middle of the base (1), and a support rotating tube (19) is rotatably connected inside the anti-slip bearing (18); and a rotating top cover (3) is fixed on the top of the supporting rotating tube (19), and the rotating top cover (3) is supported by the support tube (19). A mounting hole is provided near the circumferential edge, a rubber ring is embedded in the mounting hole, a liquid outlet pipe is inserted into the rubber ring, a valve (1102) is fixed to the top of the liquid outlet pipe, a liquid separation clepsydra (11) is fixed to the top of the valve (1102), the liquid separation clepsydra (11) comprises a vertical tube and an adjusting inclined tube (1101) obliquely inserted and connected to the vertical tube, a glass capillary (10) coaxial with the liquid outlet pipe is inserted into the top of the vertical tube, and a piston plate (27) is slidably connected to the adjusting inclined tube (1101); An internally threaded fixing ring (13) is screwed onto the circumferential outer wall of the top opening of the adjusting inclined tube (1101); spoke rods distributed centripetally are fixed onto the circumferential inner wall of the internally threaded fixing ring (13), and a common hexagonal nut is fixed in the middle of the plurality of spoke rods, in which an adjusting advancing screw (12) is screwed; a pan head is fixed onto one end of the adjusting advancing screw (12) close to the piston plate (27), and a limiting ring for clamping the pan head is fixed onto the surface of the piston plate (27); The top end of the regulating inclined tube (1101) is higher than the top end of the vertical tube, so that when the nutrient solution is added, the vertical tube of the liquid separation clepsydra (11) can be directly filled and then quickly overflow into the glass capillary (10). A drainage stick (1103) is inserted and fixed to the inner wall of the bottom end of the liquid outlet tube; A scale plate (8) is fixed to the circumferential outer wall of the glass capillary (10), and the glass capillary (10) can be replaced with different diameters according to the required filling amount, and a matching scale plate (8) is also provided therefor.

2. The mouse cell partitioning culture device according to claim 1, characterized in that: A support frame (14) is fixed on the upper surface of the rotating top cover (3) near the lower side of the adjusting inclined tube (1101), and a centripetal through hole is formed on the side of the supporting frame (14) away from the supporting rotating tube (19), and a sliding bearing is clamped in the through hole, and a transmission rod is rotatably connected in the sliding bearing; a protruding adjusting rod is reserved on the side of the valve (1102) near the supporting frame (14), and one end of the transmission rod near the adjusting rod is connected to the end of the adjusting rod through a coupling; a driven pinion (16) is fixed on the other end of the transmission rod; a toggle hand wheel (15) meshing with the driven pinion (16) is provided on the side of the supporting frame (14) near the upper side of the driven pinion (16); and the diameter of the toggle hand wheel (15) is larger than the diameter of the driven pinion (16).

3. The mouse cell partitioning culture device according to claim 1, characterized in that: The diameter of the rotating top cover (3) is equal to the diameter of the top disk of the base (1), and an annular sterile protective frame (2) is fixed to the upper surface of the top disk of the base (1) near the circumferential edge, and the annular sterile protective frame (2) is made of a transparent material, and a plurality of outlets (201) are opened on the circumferential outer wall of the annular sterile protective frame (2) near the bottom end, and the width and length of the outlets (201) are compatible with the outer dimensions of the culture dish (26) and the C-shaped clamp (2302); and a number is provided above each of the outlets (201).

4. The mouse cell partitioning culture device according to claim 3, characterized in that: The side surfaces of the outer wall of the annular sterile protection frame (2) close to the outlet (201) are hingedly connected with side covers (17), and a return torsion spring is arranged inside the hinge of the side cover (17).

5. The mouse cell partitioning culture device according to claim 1, characterized in that: A spring baffle is fixed between the end of the rectangular sliding hole (101) and the anti-slip bearing (18) on the top inner wall of the base (1), and a main tension spring (21) is fixed between the spring baffle and the vertical rod of the folding push rod (23) at the work station; an arc-shaped groove (2301) is formed on the lower surface of the end of the folding push rod (23) away from the C-shaped clip (2302); a pushing mechanism is provided in the support rotating tube (19), and the pushing mechanism includes two angle steel slide rails (20) fixed to the support rotating tube (19), the openings of which are opposite to each other and parallel to each other, and the same T-shaped slide rod (22) is slidably connected between the two angle steel slide rails (20), and the center line of the T-shaped slide rod (22) is An L-shaped stopper (2201) is fixed to one end of the T-shaped slide bar (22) close to the liquid separation clepsydra (11), and an arc-shaped stopper (2201) is arranged at one end of the L-shaped stopper (2201) away from the T-shaped slide bar (22); and two return springs (2202) are fixed to both sides of the arc-shaped stopper; one end of the two angle steel slide rails (20) close to the L-shaped stopper (2201) is fixed to a hook for fixing the return spring (2202); the lower surface of the other end of the angle steel slide rail (20) is fixed to a limiting convex column (25), and a limiting strip is inserted into the side of the T-shaped slide bar (22) close to the bottom end.

6. The mouse cell partitioning culture device according to claim 5, characterized in that: The bottom end of the support rotating tube (19) is located between the two angle steel slide rails (20) and has a roller groove, and a second fixed pulley (28) is arranged in the roller groove. A perforated anti-falling cover is fixed to the top of the support rotating tube (19), and a pull rope (9) is fixed to the upper surface of the end of the T-shaped slide bar (22) away from the L-shaped stop block (2201), and the pull rope (9) passes around the second fixed pulley (28) and passes through the perforated anti-falling cover to be fixed with a pull ring (7); a centripetally distributed Y-shaped composite roller frame (5) is fixed to the side of the upper surface of the rotating top cover (3) away from the liquid separation clepsydra (11), and a fixed pulley (6) is arranged at one end of the Y-shaped composite roller frame (5) close to the support rotating tube (19); a plurality of limiting pins (4) for sleeve-connecting the pull ring (7) are fixed to the upper surface of the other end of the Y-shaped composite roller frame (5); the distance between each limiting pin (4) and the center of the support rotating tube (19) is different.

7. The mouse cell partitioning culture device according to claim 6, characterized in that: A second magnet (29) is embedded on the upper surface of one end of the L-shaped stopper (2201) away from the T-shaped slide bar (22), and a first magnet (24) is arranged above the second magnet (29) at the bottom of the arc-shaped slot (2301) at the bottom end of the folded push rod (23) to attract the first magnet (24).

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