Reagent sampling control method and device and computer equipment
By correcting and optimizing the control logic of the reagent bottle liquid level height, the problems of insufficient reagents or empty reagents are solved, the accuracy and efficiency of reagent sampling control are achieved, and the accuracy of analysis and detection is improved.
Patent Information
- Application Number
- CN202411692124.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-25
- Publication Date
- 2025-02-21
AI Technical Summary
In the prior art, the actual volume of the reagent bottle is inconsistent with the theoretical volume, resulting in inaccurate detection of the reagent residual amount, which in turn leads to insufficient reagents or empty reagents, affecting the accuracy of the analysis and detection.
By correcting the liquid level height based on the reagent bottle volume and the preset dead cavity volume, obtaining the correspondence between the reagent residue and the distance of the reagent needle, controlling the downward flow of the reagent needle and the liquid level detection, and judging the effectiveness of the liquid level detection. And control the deceleration and stop of the reagent needle according to preset rules to ensure accurate reagent sampling.
The liquid level of reagent bottles with different volumes is achieved, which avoids insufficient reagents or empty reagents, reduces reagent waste, and improves the accuracy of analysis and detection.
Smart Images
Figure CN119492878A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of reagent sampling control, and in particular to a reagent sampling control method, device and computer equipment. Background Art
[0002] When analyzing samples, fully automatic biochemical analysis requires the addition of precise amounts of reagents to participate in the reaction. The accuracy of the reagent amount is the prerequisite for the instrument to calculate accurate results. When the reagent liquid surface approaches the bottom of the bottle, in order to ensure that the reagent needle can reliably absorb the reagent, each manufacturer will set the dead space volume V for reagent bottles of different volumes. min , when the reagent residual amount is less than V min The reagent needle will no longer absorb reagent. In order to achieve a residual volume less than V min No more pipetting of reagents, an accurate method of residual volume detection is required.
[0003] However, due to the influence of reagent bottle processing, the actual volume of the reagent bottle cannot be guaranteed to be completely consistent with the theoretical volume, which will cause the residual formula to be inconsistent with the actual situation and the detected reagent residual volume to be inaccurate, especially when the actual reagent residual volume is less than V min When the actual liquid level of the reagent is too low, due to detection errors, the reagent needle approaches or touches the bottom of the reagent bottle, which hinders the suction of sufficient reagent (insufficient reagent), or the distance that the reagent needle tip is immersed in the reagent is not enough to support the suction of sufficient reagent (reagent is sucked empty), which ultimately leads to erroneous instrument analysis results.
[0004] Therefore, the prior art still needs to be improved and developed. Summary of the invention
[0005] The technical problem to be solved by the present invention is to provide a reagent sampling control method, device and computer equipment to avoid reagent shortage or reagent vacuum phenomenon, so as to improve the accuracy of analysis and detection.
[0006] The technical solution adopted by the present invention to solve the technical problem is as follows:
[0007] A reagent sampling control method comprises the following steps:
[0008] Based on the volume V of the reagent bottle bottle and the preset reagent bottle dead space volume V min , calibrate the liquid level in the reagent bottle to obtain the corresponding relationship between the reagent remaining amount V and the distance S that the reagent needle drops from the reagent needle horizontal plane to the liquid surface;
[0009] According to the corresponding relationship, determine V min The corresponding S min ;
[0010] Control the reagent needle to move downward and perform liquid level detection to obtain the distance S' that the current reagent needle has descended from the reagent needle horizontal plane to the liquid surface;
[0011] When S'>S min When the liquid level detection is invalid, the reagent needle is controlled to stop and no sampling is performed;
[0012] When S'≤S min When the liquid level detection is determined to be a valid liquid level detection, the reagent needle is controlled to slow down and stop according to a preset rule, and sampling is performed after stopping, and the current reagent remaining amount is obtained according to the corresponding relationship.
[0013] The reagent sampling control method, wherein the reagent bottle volume V bottle and the preset reagent bottle dead space volume V min , the calibration of the liquid level in the reagent bottle specifically includes:
[0014] For volume V bottle Any reagent bottle, based on V min Determine N·V min The corresponding reagent needle drops from the reagent needle horizontal plane to the liquid surface. cal ; Where N is a multiple constant, and N ≥ 1;
[0015] For volume V bottle Another reagent bottle based on V min Determine 5N·V min The corresponding reagent needle drops from the reagent needle horizontal plane to the liquid surface S' cal ; Among them, 5N·V min ≤V bottle ;
[0016] According to S cal and S' cal , determine the slope k and intercept b; where
[0017]
[0018] The reagent sampling control method, wherein the corresponding relationship is specifically:
[0019] V = k·S + b; where k is the slope and b is the intercept.
[0020] The reagent sampling control method, wherein the control of the reagent needle to decelerate and stop according to a preset rule specifically includes:
[0021] The deceleration start liquid level is preset; wherein the deceleration start liquid level is higher than V min Corresponding liquid level;
[0022] When S'<S' d When the liquid level is detected, the reagent needle is controlled to decelerate, and the reagent needle decelerates and runs for a distance of S d After that, the reagent needle is stopped; among them, S' d S is the distance that the reagent needle travels from the reagent needle horizontal plane to the deceleration start liquid surface. d The reagent needle starts to decelerate and the liquid level moves down to V min The distance corresponding to the liquid surface;
[0023] When S' d ≤S'≤S min When the reagent needle descends from the reagent needle horizontal plane to S' d The reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min Then control the reagent needle to stop.
[0024] The reagent sampling control method, wherein the control of the reagent needle to decelerate and stop according to a preset rule specifically includes:
[0025] The deceleration start liquid level and the critical distance Δ are preset; wherein the deceleration start liquid level is higher than V min Corresponding liquid level;
[0026] When S'<S' d When the liquid level is detected, the reagent needle is controlled to decelerate, and the reagent needle decelerates and runs for a distance of S d After that, the reagent needle is stopped; among them, S' d S is the distance that the reagent needle travels from the reagent needle horizontal plane to the deceleration start liquid surface. d The reagent needle starts to decelerate and the liquid level moves down to V min The distance corresponding to the liquid surface;
[0027] When S' d ≤S'≤S min -Δ, when the reagent needle descends from the reagent needle horizontal plane to S' d The reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min Then control the reagent needle to stop;
[0028] When S min -Δ≤S'≤S min When the reagent needle descends from the reagent needle horizontal plane to S' d The reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min After +Δ, the reagent needle is controlled to stop.
[0029] The reagent sampling control method, wherein when S'>Smin When the liquid level detection is determined to be invalid, the reagent needle is controlled to stop and no sampling is performed, and the following steps are also included:
[0030] A prompt message is issued to remind the user to add reagents.
[0031] A control device based on any one of the reagent sampling control methods described above, wherein the control device comprises:
[0032] Acquisition module for the volume V of the reagent bottle bottle and the preset reagent bottle dead space volume V min , calibrate the liquid level in the reagent bottle to obtain the corresponding relationship between the reagent remaining amount V and the distance S that the reagent needle drops from the reagent needle horizontal plane to the liquid surface;
[0033] A determination module is used to determine V according to the corresponding relationship. min The corresponding S min ;
[0034] The liquid level detection module is used to control the reagent needle to move downward and perform liquid level detection to obtain the distance S' that the current reagent needle has descended from the reagent needle horizontal plane to the liquid surface;
[0035] The first control module is used when S'>S min When the liquid level detection is invalid, the reagent needle is controlled to stop and no sampling is performed;
[0036] The second control module is used when S'≤S min When the liquid level detection is determined to be a valid liquid level detection, the reagent needle is controlled to slow down and stop according to a preset rule, and sampling is performed after stopping, and the current reagent remaining amount is obtained according to the corresponding relationship.
[0037] A computer device comprises a memory and a processor, wherein the memory stores a computer program, and the processor implements the steps of any one of the methods described above when executing the computer program.
[0038] A computer-readable storage medium stores a computer program, which, when executed by a processor, implements the steps of any one of the methods described above.
[0039] A computer program product comprises a computer program, which implements the steps of any one of the methods described above when the computer program is executed by a processor.
[0040] Beneficial effect: The present application accurately calibrates the liquid level of reagent bottles of different volumes and uses optimized control logic to prevent the reagent needle from aspirating reagent when the remaining amount is insufficient, thereby preventing the reagent needle from approaching or touching the bottom of the reagent bottle due to the actual liquid level being too low, thereby hindering the suction of sufficient reagent (insufficient reagent), or the distance where the reagent needle tip is immersed in the reagent is not enough to support the suction of sufficient reagent (reagent suction is empty), thereby reducing reagent waste and improving the accuracy of reagent analysis and detection. BRIEF DESCRIPTION OF THE DRAWINGS
[0041] Figure 1 It is N·V in the present invention min The distance S between the corresponding liquid surface and the reagent needle horizontal plane cal Schematic diagram of
[0042] Figure 2 It is 5N·V in the present invention min The corresponding distance S' between the liquid surface and the reagent needle horizontal plane cal Schematic diagram of
[0043] Figure 3 is S' in the present invention d , S d and S min Schematic diagram of the corresponding relationship between them;
[0044] Figure 4 is Δ, S' in the present invention d , S d and S min Schematic diagram of the corresponding relationship between them;
[0045] Figure 5 is a flow chart of the reagent sampling control method described in the present invention;
[0046] Figure 6 It is a schematic diagram of the internal structure of the computer device described in the present invention. DETAILED DESCRIPTION
[0047] It will be understood by those skilled in the art that, unless expressly stated, the singular forms "one", "said", and "the" used herein may also include plural forms. It should be further understood that the term "comprising" used in the specification of the present application refers to the presence of the features, integers, steps, operations, elements and / or components, but does not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or groups thereof. It should be understood that when we refer to an element as being "connected" or "coupled" to another element, it may be directly connected or coupled to the other element, or there may be an intermediate element. In addition, the "connection" or "coupling" used herein may include wireless connection or wireless coupling. The term "and / or" used herein includes all or any unit and all combinations of one or more associated listed items.
[0048] It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as those generally understood by those skilled in the art to which this application belongs. It should also be understood that terms such as those defined in general dictionaries should be understood to have meanings consistent with those in the context of the prior art, and will not be interpreted with idealized or overly formal meanings unless specifically defined as here.
[0049] The present invention provides a reagent sampling control method, such as Figure 5 As shown, it includes the following steps:
[0050] S100, based on the volume of the reagent bottle V bottle and the preset reagent bottle dead space volume V min , calibrate the liquid level in the reagent bottle to obtain the corresponding relationship between the reagent remaining amount V and the distance S that the reagent needle drops from the reagent needle horizontal plane to the liquid surface;
[0051] Specifically, V bottle Represents the volume of the reagent bottle (such as 10mL, 20mL, 35mL, 70mL, etc.), V min Represents the preset dead space volume of the reagent bottle, V min Less than V bottle The dead space volume of the reagent bottle refers to the space inside the reagent bottle that cannot be fully utilized due to the design of the bottle or the process of use. Therefore, when the reagent content in the reagent bottle is equal to or even less than the dead space volume of the reagent bottle, it is not suitable for sampling, which may easily cause the reagent needle to approach or contact the bottom of the reagent bottle, hindering the suction of sufficient reagent, or the distance that the reagent needle tip is immersed in the reagent is not enough to support the suction of sufficient reagent.
[0052] The present application corrects the liquid level in the reagent bottle to obtain the corresponding relationship between the reagent remaining amount V and the distance S from the reagent needle horizontal plane to the liquid surface, and calculates the reagent remaining amount through the corresponding relationship. Reagent bottles of other volume specifications can be theoretically converted through the corresponding relationship to ultimately determine the reagent remaining amount. The corresponding relationship obtained after correcting the liquid level in the reagent bottle can ensure that reagent bottles of different specifications can obtain accurate liquid level correction.
[0053] The volume V of the reagent bottle is based on bottle and the preset reagent bottle dead space volume V min , the calibration of the liquid level in the reagent bottle specifically includes:
[0054] S101, for volume V bottle Any reagent bottle, based on V min Determine N·V min The corresponding reagent needle drops from the reagent needle horizontal plane to the liquid surface. cal ; Where N is a multiple constant, and N ≥ 1;
[0055] S102, for volume V bottle Another reagent bottle based on V min Determine 5N·V min The corresponding reagent needle drops from the reagent needle horizontal plane to the liquid surface S' cal ; Among them, 5N·V min ≤V bottle ;
[0056] S103, according to S cal and S' cal , determine the slope k and intercept b; where
[0057] and
[0058] Specifically, select any volume V bottle Into the reagent bottle, inject N·V min Amount (N is a multiple constant, and N ≥ 1) of normal saline, and control the reagent needle to move downward from the reagent needle horizontal surface until the reagent needle moves to N·V min The liquid level corresponding to the saline solution with a certain content is obtained, and the distance traveled by the reagent needle is defined as S cal (like Figure 1 shown).
[0059] Select another reagent bottle, the volume of which is still V bottle , inject 5N·V min Quantity (5N·V min ≤Vbottle ) of physiological saline, and control the reagent needle to move downward from the reagent needle horizontal surface until the reagent needle moves to 5N·V min The liquid level corresponding to the saline solution with a certain content is obtained, and the distance traveled by the reagent needle is defined as S' cal (like Figure 2 shown).
[0060] Get S cal and S' cal After that, according to S cal and S' cal , determine the slope k and intercept b, and then determine the corresponding relationship according to k and b.
[0061] The specific corresponding relationship is:
[0062] V = k·S + b;
[0063] Among them, k is the slope and b is the intercept, then the corresponding relationship between S and V is:
[0064] S = (Vb) / k;
[0065] Since k and b in the corresponding relationship are both known, S can be obtained according to V; similarly, V can also be obtained according to S; therefore, even for reagent bottles of different volumes, the reagent remaining amount can be accurately calculated.
[0066] It should be noted that a liquid level detection sensor is installed at the end of the reagent needle. According to the liquid level signal detected by the liquid level detection sensor, the distance S of the reagent needle from the reagent needle horizontal plane to the liquid surface can be obtained, and then the corresponding reagent remaining amount V can be obtained according to the corresponding relationship.
[0067] S200: Determine V according to the corresponding relationship. min The corresponding S min ;
[0068] Specifically, according to the corresponding relationship, it is determined that when V=V min S when: In the formula V = k·S + b, when V = V min When S=S min In this step, the reagent remaining amount is equal to the dead space volume of the reagent bottle, then the liquid level height is the liquid level height corresponding to the dead space volume of the reagent bottle, S min That is, it is the distance that the reagent needle moves downward from the reagent needle horizontal plane to the liquid level corresponding to the dead space volume of the reagent bottle.
[0069] S300, controlling the reagent needle to move downward and performing liquid level detection to obtain the distance S' from the reagent needle horizontal plane to the liquid surface;
[0070] Specifically, since the liquid level detection sensor is installed at the end of the reagent needle, as the reagent needle descends, the liquid level detection sensor performs liquid level detection and outputs a liquid level signal; based on the liquid level signal detected by the liquid level detection sensor, the distance traveled by the reagent needle from the horizontal plane of the reagent needle to the liquid surface can be obtained, and this distance is defined as S'.
[0071] Subsequently, the current reagent remaining amount can be known according to S' and the corresponding relationship, and the operation of the reagent needle and the sampling operation can be controlled accordingly according to the interval range to which S' belongs.
[0072] S400, when S'>S min When the liquid level detection is invalid, the reagent needle is controlled to stop and no sampling is performed;
[0073] Specifically, due to S min represents the distance from the reagent needle horizontal plane to the liquid level corresponding to the dead space volume of the reagent bottle. Therefore, when S'>S min When , it means that the current reagent content in the reagent bottle is less than the dead volume of the reagent bottle, and the liquid level in the reagent bottle is lower than the liquid level corresponding to the dead volume of the reagent bottle. It can be seen that the remaining reagent content in the reagent bottle is too low at this time. If sampling continues, the reagent needle will contact the bottom of the reagent bottle, hindering the suction of sufficient reagent, or causing the distance of the reagent needle tip immersed in the reagent to be insufficient to support the suction of sufficient reagent this time; therefore, in this application, when S'>S min When the liquid level detection is invalid, the reagent needle is controlled to stop and no sampling is performed when the liquid level is detected.
[0074] In one embodiment of the present application, when S'>S min When the liquid level detection is determined to be invalid, the reagent needle is controlled to stop and no sampling is performed, and the following steps are also included:
[0075] A prompt message is issued to remind the user to add reagents.
[0076] Specifically, since when S'>S min When the reagent content in the current reagent bottle is less than the dead space volume of the reagent bottle, it is not suitable for further sampling. Therefore, in this embodiment, after judging that the liquid level detection is an invalid liquid level detection, a prompt message will be issued to prompt the user to add reagent to avoid wasting the remaining reagent.
[0077] S500, when S'≤S min When the liquid level detection is determined to be a valid liquid level detection, the reagent needle is controlled to slow down and stop according to a preset rule, and sampling is performed after stopping, and the current reagent remaining amount is obtained according to the corresponding relationship.
[0078] Specifically, when S'≤S min , it means that the current reagent content in the reagent bottle is greater than the dead space volume of the reagent bottle, and the liquid level in the reagent bottle is higher than the liquid level corresponding to the dead space volume of the reagent bottle. Even if the reagent needle continues to move downward after detecting the liquid level, liquid aspiration and sampling can be carried out smoothly. Since the end of the reagent needle needs to be immersed in the reagent to take samples, there is a problem that the reagent needle may approach or contact the bottom of the reagent bottle and affect the aspiration operation. Therefore, in this application, after judging that the liquid level detection is a valid liquid level detection, the deceleration and stop of the reagent needle are also controlled according to the preset rules to ensure that the reagent needle will not touch the bottom of the reagent bottle under the premise of satisfying the reagent needle aspiration sampling, thereby not damaging the reagent needle and not affecting the aspiration operation of the reagent needle.
[0079] In this application, when the liquid level detection is judged to be a valid liquid level detection, the current reagent remaining amount is also obtained according to the corresponding relationship, and the current reagent remaining amount is displayed and recorded on the display screen to ensure that the staff can grasp the reagent remaining amount information in real time. Specifically, according to V=k·S+b, when S=S', the V obtained is the current reagent remaining amount.
[0080] It can be seen that the present application accurately calibrates the liquid level of reagent bottles of different volumes and uses optimized control logic to prevent the reagent needle from aspirating reagent when the remaining amount is insufficient, thereby avoiding the situation where the reagent needle approaches or touches the bottom of the reagent bottle due to the actual liquid level being too low, thereby hindering the suction of sufficient reagent (insufficient reagent), or the distance where the reagent needle tip is immersed in the reagent is insufficient to support the suction of sufficient reagent (reagent suction is empty), thereby reducing reagent waste and improving the accuracy of reagent analysis and detection.
[0081] In the first embodiment of the present application, the control of the reagent needle deceleration and stopping according to the preset rules specifically includes:
[0082] S511, presetting the deceleration start liquid level; wherein the deceleration start liquid level is higher than V min Corresponding liquid level;
[0083] S512, when S'<S' d When the liquid level is detected, the reagent needle is controlled to decelerate, and the reagent needle decelerates and runs for a distance of S d After that, the reagent needle is stopped; among them, S' d S is the distance that the reagent needle travels from the reagent needle horizontal plane to the deceleration start liquid surface. d The reagent needle starts to decelerate and the liquid level moves down to V min The distance corresponding to the liquid surface;
[0084] S513, when S' d ≤S'≤S min When the reagent needle descends from the reagent needle horizontal plane to S'd The reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min Then control the reagent needle to stop.
[0085] Specifically, the deceleration start liquid level is higher than the liquid level corresponding to the dead space volume of the reagent bottle, such as Figure 3 As shown, the distance from the deceleration start liquid level to the reagent needle level is S' d , the distance from the liquid surface corresponding to the dead volume of the reagent bottle to the horizontal plane of the reagent needle is S min The distance from the liquid surface corresponding to the dead volume of the reagent bottle to the deceleration start liquid surface is S d ; Among them, S' d With S d The sum is equal to S min In this embodiment, when S'≤S min When S' is in the state of being moved, the interval range to which S' belongs is further divided into different sections, so that different reagent needle downward control methods are executed according to the different interval ranges to which S' belongs.
[0086] When S'<S' d When the current liquid level is higher than the deceleration start liquid level, the reagent needle is controlled to start deceleration when the liquid level is detected, and the reagent needle is decelerated when the distance of the deceleration reaches S d After that, the reagent needle is controlled to stop so as to aspirate and sample, ensuring that the reagent needle can stop stably and the stopping position satisfies the reagent needle away from the bottom of the reagent bottle, thereby ensuring that the reagent needle aspirates and samples smoothly.
[0087] When S' d ≤S'≤S min When the current liquid level is lower than the deceleration starting liquid level, and the current liquid level is higher than the liquid level corresponding to the dead space volume of the reagent bottle; then, when the reagent needle descends from the reagent needle horizontal plane to the deceleration starting liquid level, the reagent needle is controlled to decelerate, and when the reagent needle decelerates to the liquid level corresponding to the dead space volume of the reagent bottle, the reagent needle is controlled to stop, ensuring that the reagent needle can stop stably and the stopping position satisfies the reagent needle to be away from the bottom of the reagent bottle, thereby ensuring that the reagent needle can smoothly aspirate and sample.
[0088] In the second embodiment of the present application, the control of the reagent needle deceleration and stopping according to the preset rules specifically includes:
[0089] S521, presetting the deceleration start liquid level and the critical distance Δ; wherein the deceleration start liquid level is higher than V min Corresponding liquid level;
[0090] S522, when S'<S' d When the liquid level is detected, the reagent needle is controlled to decelerate, and the reagent needle decelerates and runs for a distance of S d After that, the reagent needle is stopped; among them, S'd S is the distance that the reagent needle travels from the reagent needle horizontal plane to the deceleration start liquid surface. d The reagent needle starts to decelerate and the liquid level moves down to V min The distance corresponding to the liquid surface;
[0091] S523, when S' d ≤S'≤S min -Δ, when the reagent needle descends from the reagent needle horizontal plane to S' d The reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min Then control the reagent needle to stop;
[0092] S524, when S min -Δ≤S'≤S min When the reagent needle descends from the reagent needle horizontal plane to S' d The reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min After +Δ, the reagent needle is controlled to stop.
[0093] Specifically, when the liquid level in the reagent bottle drops to the liquid level corresponding to the dead volume of the reagent bottle, when the liquid level signal is detected and the reagent needle is controlled to stop at the liquid level corresponding to the dead volume of the reagent bottle, it is still impossible to ensure that the reagent needle tip remains in the liquid level after the reagent needle completes the liquid aspiration action, which may cause the reagent to be sucked empty. To avoid this phenomenon, Figure 4 As shown, in this embodiment, a critical distance Δ is introduced, where Δ is less than S d .
[0094] When S'<S' d When the current liquid level is higher than the deceleration start liquid level, the reagent needle is controlled to start deceleration when the liquid level is detected, and the reagent needle is decelerated when the distance of the deceleration reaches S d After that, the reagent needle is controlled to stop so as to aspirate and sample, ensuring that the reagent needle can stop stably and the stopping position satisfies the reagent needle away from the bottom of the reagent bottle, thereby ensuring that the reagent needle aspirates and samples smoothly.
[0095] When S' d ≤S'≤S min -Δ, the current liquid level is lower than the deceleration start liquid level and higher than S min -Δ corresponds to the liquid level, then, when the reagent needle descends from the reagent needle horizontal plane to the deceleration starting liquid level, the reagent needle is controlled to decelerate, and when the reagent needle decelerates to the liquid level corresponding to the dead space volume of the reagent bottle, the reagent needle is controlled to stop, ensuring that the reagent needle can stop stably and the stopping position satisfies the reagent needle to be away from the bottom of the reagent bottle, thereby ensuring that the reagent needle can smoothly aspirate and sample.
[0096] When Smin -Δ≤S'≤S min When the current liquid level is lower than S min -Δ corresponding to the liquid level, and higher than the liquid level corresponding to the dead volume of the reagent bottle; then, when the reagent needle descends from the reagent needle horizontal plane to reach S' d When the reagent needle descends to the deceleration start liquid level, the reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min +Δ and then control the reagent needle to stop, ensuring that the reagent needle can stop stably and that the stopping position satisfies the reagent needle to be away from the bottom of the reagent bottle, thereby ensuring that the reagent needle can smoothly aspirate and sample.
[0097] Based on any of the above reagent sampling control methods, the present application also provides a reagent sampling control device, the control device comprising:
[0098] Acquisition module for the volume V of the reagent bottle bottle and the preset reagent bottle dead space volume V min , calibrate the liquid level in the reagent bottle to obtain the corresponding relationship between the reagent remaining amount V and the distance S that the reagent needle drops from the reagent needle horizontal plane to the liquid surface;
[0099] A determination module is used to determine V according to the corresponding relationship. min The corresponding S min ;
[0100] The liquid level detection module is used to control the reagent needle to move downward and perform liquid level detection to obtain the distance S' that the current reagent needle has descended from the reagent needle horizontal plane to the liquid surface;
[0101] The first control module is used when S'>S min When the liquid level detection is invalid, the reagent needle is controlled to stop and no sampling is performed;
[0102] The second control module is used when S'≤S min When the liquid level detection is determined to be a valid liquid level detection, the reagent needle is controlled to slow down and stop according to a preset rule, and sampling is performed after stopping, and the current reagent remaining amount is obtained according to the corresponding relationship.
[0103] The present application also provides a computer device, which may be a terminal, and its internal structure diagram may be as shown in Figure 6As shown. The computer device includes a processor, a memory, an input / output interface, a communication interface, a display unit and an input device. Wherein, the processor, the memory and the input / output interface are connected via a system bus, and the communication interface, the display unit and the input device are connected to the system bus via the input / output interface. Wherein, the processor of the computer device is used to provide computing and control capabilities; the memory of the computer device includes a non-volatile storage medium and an internal memory, the non-volatile storage medium stores an operating system and a computer program, the internal memory provides an environment for the operation of the operating system and the computer program in the non-volatile storage medium, the input / output interface of the computer device is used to exchange information between the processor and the external device, and the communication interface of the computer device is used to communicate with an external terminal in a wired or wireless manner, and the wireless manner can be realized by WIFI, a mobile cellular network, NFC (near field communication) or other technologies. When the computer program is executed by the processor, the reagent sampling control method described in any one of the above is realized. The display unit of the computer device is used to form a visually visible picture, which can be a display screen, a projection device or a virtual reality imaging device. The display screen can be a liquid crystal display screen or an electronic ink display screen, and the input device of the computer device can be a touch layer covering the display screen, or a button, trackball or touchpad set on the computer device shell, or an external keyboard, touchpad or mouse.
[0104] Those skilled in the art will understand that Figure 6 The structure shown in the figure is only a block diagram of a part of the structure related to the solution of the present application, and does not constitute a limitation on the computer device to which the solution of the present application is applied. The specific computer device may include more or fewer components than those shown in the figure, or combine certain components, or have a different arrangement of components.
[0105] The present application also provides a computer-readable storage medium having a computer program stored thereon, and when the computer program is executed by a processor, the steps of any of the methods described above are implemented.
[0106] The present application also provides a computer program product, which includes a computer program, and when the computer program is executed by a processor, the steps of any one of the methods described above are implemented.
[0107] In summary, the present invention provides a reagent sampling control method.
[0108] It should be understood that the application of the present invention is not limited to the above examples. For ordinary technicians in this field, improvements or changes can be made based on the above description. All these improvements and changes should fall within the scope of protection of the claims attached to the present invention.
Claims
1. A reagent sampling control method, characterized in that: It includes the following steps: Based on the volume V of the reagent bottle bottle and the preset reagent bottle dead space volume V min , calibrate the liquid level in the reagent bottle to obtain the corresponding relationship between the reagent remaining amount V and the distance S that the reagent needle drops from the reagent needle horizontal plane to the liquid surface; According to the corresponding relationship, determine V min The corresponding S min ; Control the reagent needle to move downward and perform liquid level detection to obtain the distance S' that the current reagent needle has descended from the reagent needle horizontal plane to the liquid surface; When S ’ >S min When the liquid level detection is invalid, the reagent needle is controlled to stop and no sampling is performed; When S'≤S min When the liquid level detection is determined to be a valid liquid level detection, the reagent needle is controlled to slow down and stop according to a preset rule, and sampling is performed after stopping, and the current reagent remaining amount is obtained according to the corresponding relationship.
2. The reagent sampling control method according to claim 1, characterized in that: The volume V of the reagent bottle is based on bottle and the preset reagent bottle dead space volume V min , the calibration of the liquid level in the reagent bottle specifically includes: For volume V bottle Any reagent bottle, based on V min Determine N·V min The corresponding reagent needle drops from the reagent needle horizontal plane to the liquid surface. cal ; Where N is a multiple constant, and N ≥ 1; For volume V bottle Another reagent bottle based on V min Determine 5N·V min The corresponding reagent needle drops from the reagent needle horizontal plane to the liquid surface S' cal ; Among them, 5N·V min ≤V bottle ; According to S cal and S' cal , determine the slope k and intercept b; where 3. The reagent sampling control method according to claim 2, characterized in that: The specific corresponding relationship is: V = k·S + b; where k is the slope and b is the intercept.
4. The reagent sampling control method according to claim 1, characterized in that: The control of the reagent needle to slow down and stop according to the preset rules specifically includes: The deceleration start liquid level is preset; wherein the deceleration start liquid level is higher than V min Corresponding liquid level; When S'<S' d When the liquid level is detected, the reagent needle is controlled to decelerate, and the reagent needle decelerates and runs for a distance of S d After that, the reagent needle is stopped; among them, S' d S is the distance that the reagent needle travels from the reagent needle horizontal plane to the deceleration start liquid surface. d The reagent needle starts to decelerate and the liquid level moves down to V min The distance corresponding to the liquid surface; When S' d ≤S'≤S min When the reagent needle descends from the reagent needle horizontal plane to S' d The reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min Then control the reagent needle to stop.
5. The reagent sampling control method according to claim 1, characterized in that: The control of the reagent needle to slow down and stop according to the preset rules specifically includes: The deceleration start liquid level and the critical distance Δ are preset; wherein the deceleration start liquid level is higher than V min Corresponding liquid level; When S'<S' d When the liquid level is detected, the reagent needle is controlled to decelerate, and the reagent needle decelerates and runs for a distance of S d After that, the reagent needle is stopped; among them, S' d S is the distance that the reagent needle travels from the reagent needle horizontal plane to the deceleration start liquid surface. d The reagent needle starts to decelerate and the liquid level moves down to V min The distance corresponding to the liquid surface; When S' d ≤S'≤S min -Δ, when the reagent needle descends from the reagent needle horizontal plane to S' d The reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min Then control the reagent needle to stop; When S min -Δ≤S'≤S min When the reagent needle descends from the reagent needle horizontal plane to S' d The reagent needle is controlled to decelerate, and when the reagent needle descends from the reagent needle horizontal plane to a distance S min After +Δ, the reagent needle is controlled to stop.
6. The reagent sampling control method according to claim 1, characterized in that: When S'>S min When the liquid level detection is determined to be invalid, the reagent needle is controlled to stop and no sampling is performed, and the following steps are also included: A prompt message is issued to remind the user to add reagents.
7. A control device based on the reagent sampling control method according to any one of claims 1 to 6, characterized in that: The control device comprises: Acquisition module for the volume V of the reagent bottle bottle and the preset reagent bottle dead space volume V min , calibrate the liquid level in the reagent bottle to obtain the corresponding relationship between the reagent remaining amount V and the distance S that the reagent needle drops from the reagent needle horizontal plane to the liquid surface; A determination module is used to determine V according to the corresponding relationship. min The corresponding S min ; The liquid level detection module is used to control the reagent needle to move downward and perform liquid level detection to obtain the distance S' that the current reagent needle has descended from the reagent needle horizontal plane to the liquid surface; The first control module is used when S'>S min When the liquid level detection is invalid, the reagent needle is controlled to stop and no sampling is performed; The second control module is used when S'≤S min When the liquid level detection is determined to be a valid liquid level detection, the reagent needle is controlled to slow down and stop according to a preset rule, and sampling is performed after stopping, and the current reagent remaining amount is obtained according to the corresponding relationship.
8. A computer device, characterized in that: It comprises a memory and a processor, wherein the memory stores a computer program, and the processor implements the steps of the method according to any one of claims 1 to 6 when executing the computer program.
9. A computer-readable storage medium, characterized in that: A computer program is stored thereon, and when the computer program is executed by a processor, the steps of the method according to any one of claims 1 to 6 are implemented.
10. A computer program product, characterized in that It comprises a computer program, which, when executed by a processor, implements the steps of the method according to any one of claims 1 to 6.