Efficient rooting and seedling method for cutting slips of industrial cannabis sativa

Abstract

The invention discloses a method for efficient rooting and seedling formation of industrial hemp cutting slips. The viscous colloidal rooting agent is prepared from xanthan gum, boric acid, potassium permanganate, salicylic acid, naphthylacetic acid and indolebutyric acid, and has the advantages of being high in adhesiveness, long in acting time, stable in character, convenient to use and the like. When the rooting agent disclosed by the invention is applied to cutting slips of industrial cannabis sativa, the trimmed cutting slips of industrial cannabis sativa only need to be dipped in the sticky colloidal rooting agent, and the cutting slips are inserted into a prepared seedling culture substrate after standing for 30 minutes in a shady, cool and ventilated place. In the rooting process, humidity is increased and nutrition is supplemented through the ultrasonic humidifier with the timing function, and the problem that air humidification management is difficult in the cutting slip rooting and seedling forming process is solved. By means of the method, the industrial hemp cutting slips can root and grow seedlings within 13-16 days, and the seedling rate reaches 90% or above. The rooting rate of the industrial hemp cutting slips is greatly improved, and the method is an efficient cutting seedling raising scheme which is high in seedling rate, short in period and low in management cost.

Description

Technical Field

[0001] The invention relates to a method for efficiently rooting and seedling-forming industrial hemp cuttings, and belongs to the technical field of agricultural cultivation. Technical Background

[0002] Industrial hemp uses asexual propagation by cuttings, which can maintain all the excellent characteristics and uniformity of the mother plant. It has the characteristics of low cost and short cycle, and is suitable for large-scale, factory-based seedling cultivation.

[0003] Treating the cuttings with rooting-promoting agents and maintaining appropriate air humidity during the rooting and seedling process are key measures to improve the efficiency of industrial hemp cuttings rooting and seedling formation. On the one hand, there is a lack of special rooting-promoting agents for industrial hemp cuttings on the market. The existing general-purpose rooting-promoting agents are mainly divided into two types: powdered rooting agents and liquid rooting agents. Powdered rooting agents have problems with poor adhesion and uneven adhesion; while liquid rooting agents are easily lost with water and have the problem of poor durability. On the other hand, maintaining appropriate air humidity during the rooting and seedling process of cuttings can be achieved through artificial spraying or artificial climate chamber adjustment. Using artificial spray to increase air humidity is time-consuming and labor-intensive, while using an artificial climate chamber to adjust air humidity has high requirements for equipment.

[0004] Based on this, it is urgently needed in the industry to propose an efficient cutting seedling raising program for industrial hemp that greatly improves the rooting rate of cuttings, has a high seedling rate, a short cycle and low management costs. Summary of the invention

[0005] In view of the above-mentioned shortcomings, the present invention is on the one hand to use xanthan gum as an important material, and integrates the medicament with disinfection and rooting promotion effect, and is mixed with a homogeneous and stable viscous colloid rooting agent. The viscous colloid rooting agent is stable in nature, can be attached to the base of the cuttings quickly, evenly, effectively and durably, releases effective substances evenly and slowly in the seedling process, accelerates the cutting rooting speed, inhibits pathogen infection, and reduces the stem rot and dead seedlings caused by pathogen infection. On the other hand, the present invention is directed to the problem of difficult air humidity management in the cutting rooting process, and nutrient solution is atomized by an ultrasonic humidifier with a timing function, and the nutrient solution after atomization can not only increase air humidity quickly, but also provide nutrients for the cuttings by blades. Intermittent humidification is carried out by an ultrasonic humidifier with a timing function, and an environment of alternating dry and wet can also be created for the cuttings, promoting the differentiation and growth of roots.

[0006] The present invention is achieved by the following technical means:

[0007] The present invention first discloses a rooting agent for efficient rooting and seedling formation of industrial hemp cuttings, comprising:

[0008] 17-21 parts of xanthan gum, 1.5-3.0 parts of boric acid, 0.1-0.3 parts of potassium permanganate, 1-3 parts of salicylic acid, 0.1-0.3 parts of naphthylacetic acid, 0.1-0.3 parts of indolebutyric acid, 800-1200 parts of sterile water.

[0009] Furthermore, the rooting agent comprises:

[0010] 17-21 parts of xanthan gum, 1.5-3.0 parts of boric acid, 0.1-0.3 parts of potassium permanganate, 1-3 parts of salicylic acid, 0.1-0.3 parts of naphthylacetic acid, 0.1-0.3 parts of indolebutyric acid, 800-1200 parts of sterile water.

[0011] The present invention also discloses a method for efficiently rooting and seedling-forming industrial hemp cuttings, and the specific steps are as follows:

[0012] (1) Prepare a viscous gel rooting agent for promoting the rooting and seedling formation of industrial hemp cuttings. Put 17 to 21 parts of xanthan gum, 1.5 to 3.0 parts of boric acid, 0.1 to 0.3 parts of potassium permanganate, 1 to 3 parts of salicylic acid, 0.1 to 0.3 parts of naphthaleneacetic acid, and 0.1 to 0.3 parts of indolebutyric acid into a stirring container, add 800 to 1200 parts of sterile water, and stir well at room temperature to obtain a viscous gel rooting agent (such as Figure 1 shown).

[0013] (2) Prepare cutting medium. Mix peat medium and perlite in a volume ratio of 2:1, then add fungicide, add 240-260 parts by mass of 1 / 2 concentration modified Hoagland nutrient solution and 1-4 parts of sucrose solution, and finally adjust the water content to 70%-80% with sterile water. Transfer the prepared cutting medium into the seedling tray.

[0014] (3) Preparation and cuttings. Use a sharp blade or scissors to cut 10-15 cm long cuttings from the industrial hemp mother plant, cut off the excess leaves, and keep half of the top 1-2 leaves. Use a blade to cut the stem section at the base of the cutting into a smooth and flat slope at an angle of about 45°. Dip the trimmed base of the cutting into a sticky gel rooting agent (such as Figure 2 As shown), place in a cool and ventilated place for 25-35 minutes, then insert into the prepared cutting medium, the cutting depth is 3-4cm (as shown Figure 3 shown).

[0015] (4) Management of rooting and seedling formation of cuttings. Place the seedling tray with cuttings in a place with a light intensity of 50-100 μmol·m -2 ·s -1 Rooting in low light environment (such as Figure 4During the rooting process, a transparent plastic cover is used to cover the plug tray to keep moisture, and an ultrasonic humidifier with a timing function is connected to the plastic cover. 240-260 parts by weight of the modified Hoagland nutrient solution and 5-8 parts of sucrose solution are added to the ultrasonic humidifier. The ultrasonic humidifier uses high-frequency vibration to atomize the nutrient solution and transmit it to the transparent plastic cover, increasing the humidity inside the cover and providing nutrition to the leaf surface of the cuttings. The ultrasonic humidifier is set to a cycle mode of on for 5-10 minutes and off for 2 hours to maintain the air humidity at 60% to 80%.

[0016] Furthermore, in step (2), the peat matrix is ​​preferably peat soil with a thickness of 0 to 10 mm.

[0017] Further, in step (2), the formula of the improved Hoagland nutrient solution is as follows: KNO3 11-13mmol / L, Ca(NO3)2·4H2O 3-5mmol / L, MgSO4·7H2O 1-3mmol / L, KH2PO4 2-4mmol / L, FeSO4·7H2O 50-60μmol / L, EDTA-Na2 50-60μmol / L, H3BO3 45-55μmol / L, MnCl2·4H2O 8-10μmol / L, ZnSO4·7H2O 0.5-1μmol / L, CuSO4·5H2O 0.3-0.6μmol / L, (NH4)6Mo7O 24 4H2O 0.01~0.05μmol / L.

[0018] The beneficial effects of the present invention are:

[0019] The xanthan gum used in the present invention is a high molecular sugar substance with significant thickening and moisturizing effects, which can make the rooting agent more evenly and persistently attached to the base of the cuttings, promote the rooting agent to penetrate into the cells of the callus part, and accelerate the differentiation and growth of the root system. Xanthan gum can also have good stability, and can delay the progress of changes such as volatilization, oxidation, and decomposition of the effective substances in the rooting agent, thereby extending the effectiveness of the rooting agent. Boric acid can promote the development of the roots of the cuttings and promote the growth of meristems; naphthylacetic acid and indolebutyric acid can promote the dedifferentiation of the thin-walled cells at the base of the cuttings, restore their meristem ability, and promote root differentiation and growth.

[0020] The invention is characterized in that the rooting process of industrial hemp cuttings is sensitive to exogenous substances and air humidity, and a viscous gel rooting agent with disinfection and rooting promotion functions and strong adhesion is prepared, and air humidity is increased and nutrition is provided by an ultrasonic atomizer with a timing function, so as to significantly promote the rooting rate and seedling rate of industrial hemp cuttings.

[0021] The invention is suitable for all industrial hemp varieties, the entire seedling raising process is easy to manage, the seedling raising period is short, and it is suitable for the rapid propagation of industrial hemp plants. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a sticky gel rooting agent for industrial hemp cuttings;

[0023] Figure 2 For the cuttings dipped in the sticky gelatinous rooting agent of industrial hemp;

[0024] Figure 3 For plants that have been cut and propagated;

[0025] Figure 4 A complete set of cutting rooting equipment;

[0026] Figure 5 The rooting conditions of Yunma No. 8 cuttings treated with clean water and rooting agent in Example 1 of the present invention were compared 10 days after cutting;

[0027] Figure 6 The rooting conditions of Yunma No. 8 cuttings treated with clean water and rooting agent in Example 1 of the present invention were compared 13 days after cutting;

[0028] Figure 7 The rooting conditions of Yunma No. 8 cuttings treated with clean water and rooting agent in Example 1 of the present invention were compared 16 days after cutting;

[0029] Figure 8 The Yunma No. 8 plants were transplanted 16 days after the cuttings in Example 1 of the present invention.

[0030] Fig. 9 The rooting conditions of high CBD industrial hemp cuttings treated with clean water and rooting agent in Example 2 of the present invention are compared 10 days after cutting;

[0031] Fig.10 The rooting conditions of high CBD industrial hemp cuttings treated with clean water and rooting agent in Example 2 of the present invention after 13 days of cuttings are compared;

[0032] Fig.11 The rooting conditions of high CBD industrial hemp cuttings treated with clean water and rooting agent 16 days after cutting in Example 2 of the present invention are compared;

[0033] Fig.12 The high CBD industrial hemp plants were transplanted 16 days after cutting according to Example 2 of the present invention. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Embodiment 1:

[0036] Cultivation of cutting seedlings of "Yunma No. 8"

[0037] 1. Weigh 20 parts of xanthan gum, 2 parts of boric acid, 0.2 parts of potassium permanganate, 1 part of salicylic acid, 0.2 parts of naphthylacetic acid, and 0.2 parts of indolebutyric acid by mass.

[0038] 2. Put boric acid, potassium permanganate, salicylic acid, xanthan gum, naphthylacetic acid and indolebutyric acid into a stirring container in proportion, then add 1000 parts of sterile water, stir thoroughly at room temperature to obtain a viscous gel-like rooting agent.

[0039] 3. Mix the peat matrix and perlite in a volume ratio of 2:1 as the cutting matrix. Add cyprodinil to the cutting matrix for sterilization, and mix KNO3, Ca(NO3)2·4H2O, MgSO4·7H2O, KH2PO4, FeSO4·7H2O, EDTA-Na2, H3BO3, MnCl2·4H2O, ZnSO4·7H2O, CuSO4·5H2O, (NH4)6Mo7O 24 · Wet the cutting medium with the nutrient solution made of 4H2O and sucrose, and adjust the water content to 70%-80% with sterile water. Fill the seedling box with the prepared cutting medium.

[0040] 4. Select 40 semi-lignified cuttings from the "Yunma No. 8" mother plant that is healthy, uniform, and free of pests and diseases in the greenhouse of the Institute of Resource Plants of Yunnan University. Cut the selected cuttings to 10-15 cm, cut the lower part of the cuttings into a 45° angle, and the cut should be smooth without tearing. Keep one bud on each cutting, keep 1-2 fully expanded leaves at the top, and cut off 1 / 2 of the leaves. Avoid damage to the top buds and stems of the cuttings during pruning.

[0041] 5. Dip the trimmed cuttings into clean water and the prepared viscous gel rooting agent for 20 plants each, place them in a cool and ventilated place for 30 minutes, and then insert them into the cutting medium. The insertion depth is 3 to 4 cm. The cuttings should be inserted in one go and should not be repeatedly pulled out and inserted. Then spray sterile water evenly on the surface of the cuttings, and cover the seedling tray with a transparent plastic cover connected to a humidifier. KNO3, Ca(NO3)2·4H2O, MgSO4·7H2O, KH2PO4, FeSO4·7H2O, EDTA-Na2, H3BO3, MnCl2·4H2O, ZnSO4·7H2O, CuSO4·5H2O, (NH4)6Mo7O 24 · Add the nutrient solution made of 4H2O and sucrose to the humidifier for later use. Set the humidifier to humidify the cuttings for 10 minutes every 2 hours.

[0042] 6. Place the seedling tray in the greenhouse of the Institute of Plant Resources of Yunnan University and set the light intensity to 100 μmol·m -2 ·s -1 , daily lighting time is 16 hours.

[0043] 7. The cuttings treated with the sticky gelatinous industrial hemp cutting rooting agent began to take root in about 13 days (such as Figure 6 The rooting survival rate was 100%. After 16 days, the number of roots reached 14 to 18, the longest root length reached 8.3 cm, the root hairs were vigorous, and 1 to 2 tender leaves grew on the top (as shown in Figure 2). Figure 7 The survival rate after transplanting was 100% (as shown in Figure 8 The cuttings treated with clean water began to take root in about 16 days, and the survival rate of the cuttings was 90%. The leaves were severely yellowed (as shown in Figure 7 shown).

[0044] Embodiment 2:

[0045] Cultivation of high CBD industrial hemp plant cuttings

[0046] 1. Weigh 20 parts of xanthan gum, 2 parts of boric acid, 0.2 parts of potassium permanganate, 1 part of salicylic acid, 0.2 parts of naphthylacetic acid, and 0.2 parts of indolebutyric acid by mass.

[0047] 2. Put boric acid, potassium permanganate, salicylic acid, xanthan gum, naphthylacetic acid and indolebutyric acid into a stirring container in proportion, add 1000 parts of sterile water, stir thoroughly at room temperature to obtain a viscous gel-like rooting agent.

[0048] 3. Mix the peat matrix and perlite in a volume ratio of 2:1 as the cutting matrix. Add cyprodinil to the cutting matrix for sterilization, and mix KNO3, Ca(NO3)2·4H2O, MgSO4·7H2O, KH2PO4, FeSO4·7H2O, EDTA-Na2, H3BO3, MnCl2·4H2O, ZnSO4·7H2O, CuSO4·5H2O, (NH4)6Mo7O 24 · Wet the cutting medium with the nutrient solution made of 4H2O and sucrose. Fill the seedling box with the prepared cutting medium.

[0049] 4. Select 40 semi-lignified cuttings from the mother plants of high CBD industrial hemp plants that are healthy, uniform, and free of pests and diseases in the greenhouse of the Institute of Resource Plants of Yunnan University. Cut the selected cuttings to 10-15 cm, cut the lower part of the cuttings into a 45° angle, and the cut should be smooth and without tearing. Keep one bud on each cutting, keep 1-2 fully expanded leaves at the top, and cut off 1 / 2 of the leaves. Avoid damage to the top buds and stems of the cuttings during pruning.

[0050] 5. Dip the trimmed cuttings into clean water and the prepared viscous gel rooting agent for 20 plants each, wait for 25 minutes and insert them into the seedling pots. The cuttings should be inserted in one go and cannot be repeatedly pulled out and inserted. Place the seedling pots neatly into the seedling tray, then spray sterile water evenly on the surface of the cuttings, and cover the seedling tray with a transparent plastic cover connected to a humidifier. KNO3, Ca(NO3)2·4H2O, MgSO4·7H2O, KH2PO4, FeSO4·7H2O, EDTA-Na2, H3BO3, MnCl2·4H2O, ZnSO4·7H2O, CuSO4·5H2O, (NH4)6Mo7O 24 · Add the nutrient solution made of 4H2O and sucrose to the humidifier for later use. Set the humidifier to humidify the cuttings for 8 minutes every 2 hours.

[0051] 6. Place the seedling tray in the greenhouse of the Institute of Plant Resources of Yunnan University and set the light intensity to 80 μmol·m -2 ·s -1 , daily lighting time is 16 hours.

[0052] 7. The cuttings treated with the sticky gel rooting agent for industrial hemp cuttings began to take root in about 10 days (such as Fig. 9 The survival rate of the cuttings was 100%. After 16 days, the number of roots reached 23 to 28, the longest root length reached 25.4 cm, the root hairs were vigorous, and 1 to 2 tender leaves grew on the top (as shown in Figure 2). Fig.11 The survival rate after transplanting was 100% (as shown in Fig.12 The cuttings treated with clean water began to take root after about 13 days (as shown in Fig.10The survival rate of the cuttings was 80%. After 16 days, the number of roots reached 1 to 4, with the longest root length being 3.5 cm. The conditions for transplanting were not met (as shown in Figure 2). Fig.11 shown).

[0053] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to the above embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A rooting agent for efficient rooting and seedling formation of industrial hemp cuttings, comprising: 17-21 parts of xanthan gum, 1.5-3.0 parts of boric acid, 0.1-0.3 parts of potassium permanganate, 1-3 parts of salicylic acid, 0.1-0.3 parts of naphthylacetic acid, 0.1-0.3 parts of indolebutyric acid, 800-1200 parts of sterile water.

2. The rooting agent for efficient rooting and seedling formation of industrial hemp cuttings according to claim 1, comprising: 17-21 parts of xanthan gum, 1.5-3.0 parts of boric acid, 0.1-0.3 parts of potassium permanganate, 1-3 parts of salicylic acid, 0.1-0.3 parts of naphthylacetic acid, 0.1-0.3 parts of indolebutyric acid, 800-1200 parts of sterile water.

3. A method for efficiently rooting and seedling-forming industrial hemp cuttings, comprising: (1) Preparation of rooting agent: Put 17-21 parts of xanthan gum, 1.5-3.0 parts of boric acid, 0.1-0.3 parts of potassium permanganate, 1-3 parts of salicylic acid, 0.1-0.3 parts of naphthaleneacetic acid, and 0.1-0.3 parts of indolebutyric acid into a stirring container, add 800-1200 parts of sterile water, stir well at room temperature, and obtain a rooting agent for use; (2) Preparation of cutting medium: Mix the peat matrix and perlite evenly, then add the fungicide, add 240-260 parts by mass of 1 / 2 concentration modified Hoagland nutrient solution and 1-4 parts of sucrose solution, finally adjust the water content, and transfer the prepared cutting matrix into the seedling hole tray; (3) Preparation and cuttings: Cut 10-15cm long cuttings from the industrial hemp mother plant, cut off the excess leaves, keep half of the top 1-2 unfolded tender leaves, use a blade to cut the stem section at the base of the cuttings into a smooth and flat slope, dip the trimmed base of the cuttings into a sticky gel rooting agent, place it in a cool and ventilated place, and then insert it into the prepared cutting medium; (4) Management of rooting and seedling formation of cuttings: Place the seedling tray with cuttings in a weak light environment for rooting, use a transparent plastic cover to cover the tray to keep it moist, and connect an ultrasonic humidifier with a timing function to the plastic cover. Add 240-260 parts of improved Hoagland nutrient solution and 5-8 parts of sucrose solution to the ultrasonic humidifier to maintain the air humidity.

4. The method for efficiently rooting and seedling-forming industrial hemp cuttings according to claim 3, wherein: The volume ratio of the peat matrix to perlite in step (2) is 2:1; The water content is adjusted by using sterile water to adjust the water content to 70% to 80%.

5. The method for efficiently rooting and seedling-forming industrial hemp cuttings according to claim 3, wherein: The peat matrix in step (2) is peat soil with a thickness of 0 to 10 mm.

6. The method for efficiently rooting and seedling-forming industrial hemp cuttings according to claim 3, wherein: Step (3) is to stand in a cool and ventilated place for 25-35 minutes; The cutting depth is 3-4 cm.

7. The method for efficiently rooting and seedling-forming industrial hemp cuttings according to claim 3, wherein: The illumination intensity in step (4) is 50-100 μmol·m -2 ·s -1 .

8. The method for efficiently rooting and seedling-forming industrial hemp cuttings according to claim 3, wherein: The ultrasonic humidifier in step (4) is set to a cycle mode of on for 5-10 minutes and off for 2 hours; The air humidity is maintained at 60% to 80%.

9. The method for efficiently rooting and seedling-forming industrial hemp cuttings according to claim 3, wherein: The formula of the improved Hoagland nutrient solution is as follows: KNO311~13mmol / L、Ca(NO3)2·4H2O 3~5mmol / L、MgSO4·7H2O 1~3mmol / L、KH2PO42~4mmol / L、FeSO4·7H2O 50~60µmol / L、EDTA-Na250~60µmol / L、H3BO345~55µmol / L、MnCl2·4H2O 8~10µmol / L、ZnSO4·7H2O 0.5~1µmol / L SO4·5H2O 0.3~0.6μmol / L、(NH4)6Mo7O 24 ·4H2O 0.01~0.05μmol / L。

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