Preparation method of kelp carbon quantum dot absorbable mask
By preparing kelp into carbon quantum dots and combining biocellulose, a mask with efficient absorption and skin care effect was prepared, which solved the problem of insufficient absorption and skin care effect of existing masks, and achieved the requirements of effective utilization of marine resources and environmental protection.
Patent Information
- Application Number
- CN202411710228.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-27
- Publication Date
- 2025-02-25
AI Technical Summary
The existing facial masks have insufficient absorption and skin care effects, and have failed to effectively utilize marine biological resources.
Carbon quantum dots are prepared by preparing kelp into carbon quantum dots and combining them with components such as biocellulose. The method includes carbonization of kelp powder, preparation of carbon quantum dots, fermentation and mixing of biocellulose, and ultimately forming a mask with efficient absorption and skin care effects.
It has achieved efficient absorption of facial masks and improved skin care effects, met people's demand for efficient and safe skin care products, and at the same time effectively utilized marine resources, meeting the requirements of environmental protection and sustainable development.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of marine biological resource utilization and cosmetics, and specifically relates to a method for preparing a kelp carbon quantum dot absorbable facial mask. Background Art
[0002] As people's demand for beauty and skin care increases, facial masks, as a common skin care product, are gaining more and more attention for their functions and effects. Carbon quantum dots, as a new type of nanomaterial, have broad application prospects in the biomedical field due to their unique biocompatibility and good absorption efficiency. Kelp, as a common marine algae, is rich in a variety of bioactive substances and has good biocompatibility and antioxidant properties. Therefore, the preparation of kelp into The application of carbon quantum dots in the preparation of facial masks to achieve efficient absorption of the masks and improve the skin care effect has not been reported so far. Summary of the invention
[0003] In view of the problems existing in the prior art, the purpose of the present invention is to provide a method for preparing a kelp carbon quantum dot absorbable facial mask, which is specifically achieved through the following technical solutions: A method for preparing a kelp carbon quantum dot absorbable facial mask comprises the following steps: 1) Wash fresh kelp with deionized water three times, dry it at 60℃ for 24 hours, and grind it into kelp powder; 2) Carbonizing kelp powder at 500° C. for 2 hours to obtain kelp carbon material, dispersing the kelp carbon material in high-purity water to obtain a kelp carbon aqueous suspension, adding chemical reagents to the kelp carbon aqueous suspension, and removing unreacted chemical reagents through dialysis after hydrothermal reaction to obtain carbon quantum dots; 3) taking the kelp powder from step 1) and mixing it with water at a volume ratio of 1:1-3 and extracting it for 12 hours, filtering and centrifuging the extract to remove the fixed residue, thereby obtaining a kelp extract; 4) Mixing and fermenting the kelp extract, biological fermentation agent, glucose, yeast extract powder, peptone, MgSO4, Na2HPO4, acetic acid and ethanol, and after the fermentation, washing and drying the formed biocellulose; 5) taking the biocellulose treated in step 4) as a facial mask matrix material, mixing it with water in a certain proportion to prepare a facial mask matrix solution; 6) The carbon quantum dots prepared in step 2) are uniformly dispersed in the mask matrix solution, and auxiliary agents are added and stirred. The stirred mixture is poured into the mask mold, and the carbon quantum dot absorbable mask is obtained through drying and cutting processes.
[0004] Furthermore, in step 2), the volume ratio of the kelp carbon material to high-purity water is 1:3-7; the volume ratio of the kelp carbon water suspension to the chemical reagent is 4-6:1.
[0005] Furthermore, in step 2), the chemical reagent is any one of 4 mol / L nitric acid or 36-38 wt% hydrochloric acid.
[0006] Furthermore, in step 2), the temperature of the hydrothermal reaction is 80° C., and the time of the hydrothermal reaction is 2 hours.
[0007] Furthermore, in step 4), the mass percentages of the raw materials for forming biocellulose are: 75-85% kelp extract, 8-12% biological fermentation agent, 2-6% glucose, 0.5-1.5% yeast extract, 0.6-1.2% peptone, 1-3% MgSO4, 0.1-0.3% Na2HPO4, 0.2-0.6% acetic acid, and 1.4-1.8% ethanol. Preferably, 80% kelp extract, 10% biological fermentation agent, 4% glucose, 1% yeast extract, 0.8% peptone, 2% MgSO4, 0.2% Na2HPO4, 0.4% acetic acid, and 1.6% ethanol.
[0008] Furthermore, in step 5), the volume ratio of the mask matrix material to water is 4:6.
[0009] Furthermore, in step 6), the amount of carbon quantum dots added is 4% by weight of the mask matrix solution.
[0010] The preparation method of the present invention is simple in process and easy to operate, suitable for large-scale industrial production, and uses kelp to prepare carbon quantum dots, realizing the effective utilization of marine resources, while improving the biocompatibility and antioxidant properties of the mask. The addition of carbon quantum dots enables the mask to have better absorption performance and skin care effects, and can meet people's demand for efficient and safe skin care products.
[0011] The biofiber mask base material made from kelp combines the natural ingredients of kelp and the excellent properties of biofiber, which can provide rich nutrition and deep care for the skin. At the same time, due to the environmental protection of kelp itself, this mask base material also meets the modern people's pursuit of environmental protection and sustainable development. DETAILED DESCRIPTION
[0012] The present invention is further described below in conjunction with specific embodiments to facilitate a better understanding of the present technical solution. Example
[0013] The fresh kelp was washed with deionized water for 3 times, then dried at 60°C for 24 hours and crushed into powder. The kelp powder was carbonized at 500°C for 2 hours to obtain kelp carbon material. The kelp carbon material was dispersed in high-purity water at a volume ratio of 1:5, and then chemical reagents (4 mol / L nitric acid) were added at a ratio of 5:1, mixed, and hydrothermally reacted at 80°C for 2 hours, and then the unreacted chemical reagents were removed by dialysis to obtain carbon quantum dots.
[0014] The kelp powder is mixed with water or an appropriate solvent in a volume ratio of 1:2 and soaked for 12 hours to extract the active ingredients in the kelp. The solid residue is removed by filtering, centrifuging and other processes to obtain the kelp extract.
[0015] 80% kelp extract, 10% biological fermentation agent (such as Acetobacter xylinum), 4% glucose, 1% yeast extract powder, 0.8% peptone, 2% MgSO4, 0.2% Na2HPO4, 0.4% acetic acid, and 1.6% ethanol were mixed and fermented. The temperature was controlled at 25-30°C and fermented for 1 week. After the fermentation, the formed biocellulose was washed and dried. Biocellulose was selected as the mask matrix material and mixed with water in a volume ratio of 4:6 to prepare a mask matrix solution. The prepared carbon quantum dots were evenly dispersed in the mask matrix solution at a mass ratio of 4%, and other skin care ingredients were added, such as moisturizers (sodium hyaluronate 2%, glycerin 5%, etc.), antioxidants (vitamin C derivatives 2%, green tea extract 1%, etc.) thickeners (xanthan gum 2%, carbomer 2%), etc. The carbon quantum dots and other ingredients were evenly dispersed by stirring. Finally, the mixed solution was poured into the mask mold, and after drying, cutting and other processes, a carbon quantum dot absorbable mask was obtained. Example
[0016] On the basis of Example 1, the preparation process of carbon quantum dots was optimized to reduce the reaction preparation cost and improve the safety of the product. The fresh kelp was washed with deionized water 3 times, then dried at 60°C for 24 hours and crushed into powder. The kelp powder was carbonized at 500°C for 2 hours to obtain the kelp carbon material. The kelp carbon material was dispersed in high-purity water at a volume ratio of 1:5, and then a chemical reagent (36-38 wt% hydrochloric acid) was added at a volume ratio of 5:1. After mixing the hydrochloric acid, it was ultrasonically treated for 4 hours under a 300 W ultrasonic instrument to obtain carbon quantum dots.
[0017] Mix kelp powder with water or appropriate solvent in a volume ratio of 1:2 and soak for 12 hours to extract the active ingredients in the kelp. The solid residue is removed to obtain the kelp extract.
[0018] 80% kelp extract, 10% biological fermentation agent (such as Acetobacter xylinum), 4% glucose, 1% yeast extract powder, 0.8% peptone, 2% MgSO4, 0.2% Na2HPO4, 0.4% acetic acid, and 1.6% ethanol were mixed and fermented. The temperature was controlled at 25-30°C and fermented for 1 week. After the fermentation, the formed biocellulose was washed and dried. Biocellulose was selected as the mask matrix material and mixed with water in a volume ratio of 4:6 to prepare a mask matrix solution. The prepared carbon quantum dots were evenly dispersed in the mask matrix solution at a mass ratio of 4%. At the same time, 5% arbutin was added to the mask matrix to enhance the whitening effect of the mask, antioxidants (2% vitamin C derivatives, 1% green tea extract, etc.) thickeners (2% xanthan gum, 2% carbomer), etc. The carbon quantum dots and other ingredients were evenly dispersed by stirring. Finally, the mixed solution was poured into the mask mold, and after drying, cutting and other processes, a carbon quantum dot absorbable mask was obtained.
[0019] Test Example 1: Test of skin moisture content The measurement was performed using a Corneometer skin moisture measuring instrument. A male tester, 20 years old, with a skin type of neutral to dry, was selected. The same skin area was measured using a Corneometer before and after applying the mask prepared in Example 1. By comparing the skin moisture content of 12% before applying the mask, the skin moisture content was 15.6% after 20 minutes, and the percentage increase in moisture content was calculated as (15.6%-12%) / 12%=30%.
[0020] Test Example 2: Cell Viability Test The CCK-8 cell activity assay was used to evaluate cell viability. The mask extract was co-cultured with skin keratinocytes, and then the CCK-8 reagent was added. The absorbance at 450nm was measured by an ELISA instrument, and the cell viability was calculated according to the formula:
[0021] The facial mask extract is specifically prepared by breaking the facial mask into small pieces, mixing the pieces with water in a 1:1 ratio, soaking the pieces under ultrasonication for 20 minutes at room temperature, and centrifuging to obtain the supernatant, which is the facial mask extract.
[0022] Among them, OD value refers to the optical density value. The OD value of the experimental group is the absorbance value of the cells after the mask extract is co-cultured with the cells, the OD value of the control group is the absorbance value of the cells without the above treatment, and the OD value of the blank control group is the absorbance of the culture medium. After testing: the OD value of the experimental group is 1.2, the OD value of the blank control group is 0.3, and the OD value of the control group is 1.23. After calculation:
[0023] Test Example 3: DPPH free radical scavenging rate The UV-visible spectrophotometry was used. The mask extract was mixed with the DPPH free radical solution, and the absorbance change at 517nm or 515nm was measured after 20 minutes. The DPPH free radical scavenging rate was calculated according to the formula: DPPH free radical scavenging rate (%) = (A control - A sample) / A control × 100% = (0.8-0.24) / 0.8 = 70% Among them, A control is the absorbance of DPPH free radical when no sample is added, that is, the absorbance of the control group, and A sample is the absorbance of DPPH free radical after adding the sample.
[0024] Test Example 4: Skin irritation test and sensitivity test The skin irritation test was conducted using the ISO 10993-23:2021 standard, and the mask extract was placed in direct contact with the animal skin for 20 minutes to observe its potential skin irritation reaction. Skin erythema and edema were observed at different time points (1h, 24h, 48h and 72h) after the removal of the patch. No adverse reactions were found after testing.
[0025] The sensitivity test used a patch test, in which the mask was applied to the skin of the volunteers and observed after 20 minutes for allergic reactions, such as papules, erythema, edema, etc. No allergic reactions were observed.
Claims
1. A method for preparing a kelp carbon quantum dot absorbable facial mask, characterized in that The preparation method comprises the following steps: 1) Wash fresh kelp with deionized water three times, dry it at 60℃ for 24 hours, and grind it into kelp powder; 2) Carbonizing kelp powder at 500° C. for 2 hours to obtain kelp carbon material, dispersing the kelp carbon material in high-purity water to obtain a kelp carbon aqueous suspension, adding chemical reagents to the kelp carbon aqueous suspension, and removing unreacted chemical reagents through dialysis after hydrothermal reaction to obtain carbon quantum dots; 3) taking the kelp powder from step 1) and mixing it with water at a volume ratio of 1:1-3 and extracting it for 12 hours, filtering and centrifuging the extract to remove the fixed residue, thereby obtaining a kelp extract; 4) Combine kelp extract, biofermentation agent, glucose, yeast extract powder, peptone, MgSO 4、 Na2HPO4, acetic acid and ethanol are mixed and fermented, and after the fermentation is completed, the formed biocellulose is washed and dried; 5) taking the biocellulose treated in step 4) as a facial mask matrix material, mixing it with water in a certain proportion to prepare a facial mask matrix solution; 6) The carbon quantum dots prepared in step 2) are uniformly dispersed in the mask matrix solution, and auxiliary agents are added and stirred. The stirred mixture is poured into the mask mold, and the carbon quantum dot absorbable mask is obtained through drying and cutting processes.
2. A method for preparing a kelp carbon quantum dot absorbable facial mask as claimed in claim 1, characterized in that In step 2), the volume ratio of kelp carbon material to high-purity water is 1:3-7; the volume ratio of kelp carbon water suspension to chemical reagent is 4-6:
1.
3. A method for preparing a kelp carbon quantum dot absorbable facial mask as claimed in claim 1, characterized in that In step 2), the chemical reagent is any one of 4 mol / L nitric acid or 36-38 wt% hydrochloric acid.
4. A method for preparing a kelp carbon quantum dot absorbable facial mask as claimed in claim 1, characterized in that In step 2), the temperature of the hydrothermal reaction is 80° C., and the time of the hydrothermal reaction is 2 hours.
5. A method for preparing a kelp carbon quantum dot absorbable facial mask as claimed in claim 1, characterized in that In step 4), the mass percentages of the raw materials forming the biocellulose are: kelp extract 75-85%, biological fermentation agent 8-12%, glucose 2-6%, yeast extract powder 0.5-1.5%, peptone 0.6-1.2%, MgSO4 1-3%, Na2HPO4 0.1-0.3%, acetic acid 0.2-0.6%, and ethanol 1.4-1.8%.
6. A method for preparing a kelp carbon quantum dot absorbable facial mask as claimed in claim 5, characterized in that The mass percentages of the raw materials forming the biocellulose are: kelp extract 80%, biological fermentation agent 10%, glucose 4%, yeast extract powder 1%, peptone 0.8%, MgSO4 2%, Na2HPO4 0.2%, acetic acid 0.4%, and ethanol 1.6%.
7. A method for preparing a kelp carbon quantum dot absorbable facial mask as claimed in claim 1, characterized in that In step 5), the volume ratio of the mask matrix material to water is 4:
6.
8. A method for preparing a kelp carbon quantum dot absorbable facial mask as claimed in claim 1, characterized in that In step 6), the amount of carbon quantum dots added is 4% by weight of the mask matrix solution.