Traditional Chinese medicine ointment for clearing heat, relieving cough and reducing sputum and preparation process thereof

Abstract

The invention belongs to the field of traditional Chinese medicines, mainly relates to a traditional Chinese medicine ointment and a preparation process thereof, and particularly relates to a traditional Chinese medicine ointment for clearing heat, relieving cough and reducing sputum and a preparation process thereof. According to the invention, indigo naturalis, cortex lycii radicis, gypsum, scutellaria baicalensis, radix stemonae, tabasheer, raw ephedra, platycodon grandiflorum and lalang grass rhizome are used as a traditional Chinese medicine prescription, glycerin monostearate, paraffin, liquid paraffin, albolene, span 80, an OP emulsifier, chlorocresol and distilled water are used as auxiliary materials, bacillus megatherium CICC 24484 and aspergillus oryzae CICC2066 are used for carrying out synergistic fermentation on platycodon grandiflorum and lalang grass rhizome, and then decoction is carried out for multiple times. The traditional Chinese medicinal materials are more beneficial to exerting the effects, the traditional Chinese medicinal composition has important significance for treating symptoms such as cough and excessive phlegm caused by internal heat, and meanwhile, an unexpected effect of relieving asthma is shown due to synergistic fermentation.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and mainly relates to a traditional Chinese medicine ointment and a preparation process thereof, and specifically relates to a traditional Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm and a preparation process thereof. Background Art

[0002] Coughing and expectoration are common symptoms of upper respiratory tract infection, and both are considered to be defense mechanisms of the respiratory system. Coughing is a protective respiratory reflex that promotes airway hygiene. Coughing occurs when mucus secretion increases, inflammation, etc. lead to ineffective mucociliary clearance. Sputum is caused by inflammation, and it may include mucus with bacteria, inflammatory cells, and inflammatory mediators. When airway mucus secretion is excessive and lasts for too long, it may lead to serious respiratory diseases such as asthma and chronic obstructive pulmonary disease. People with chronic obstructive pulmonary disease are prone to chest infections due to excessive mucus secretion, which can endanger their lives in severe cases. In order to relieve severe cough and sputum, Western medicine often uses antitussive and expectorant, antihistamine, antibiotics and other drugs for treatment. Although they have certain clinical efficacy, the condition is prone to recurrence and has obvious toxic and side effects. Traditional Chinese medicine has a unique advantage in treating chronic cough. Traditional Chinese medicine not only focuses on local symptoms, but also starts from the whole body condition, considering the patient's constitution, environmental factors, living habits, etc., and adjusts the patient's constitution to enhance the body's resistance and self-repair ability, thereby reducing the onset of chronic cough. Traditional Chinese medicine methods for treating cough with phlegm or chronic cough usually have fewer side effects and are more suitable for long-term treatment. Summary of the invention

[0003] In view of the above reasons, the present invention provides a Chinese medicinal ointment for clearing away heat, relieving cough and reducing phlegm and a preparation process thereof, which specifically comprises the following steps:

[0004] S1. Preparation of materials: Chinese medicine prescription: put 30-35g of Indigo Naturalis, 60-70g of Radix Lycii, 20-30g of Gypsum, 60-65g of Radix Scutellariae, 100-110g of Radix Stemonae, 60-70g of Radix Daphne, 30-40g of Ephedrae Herba, 30-40g of Platycodon Grandiflorum and 100-110g of Rhizoma Imperatae into a wall-breaking machine for grinding; auxiliary materials: 15-30g of glyceryl monostearate, 15-30g of paraffin, 70-80g of liquid paraffin (heavy), 15-20g of white vaseline, 1-2g of Span 80, 1-2g of OP emulsifier, 0.6-1.2g of chlorocresol and 50-100g of distilled water;

[0005] S2. Preparation of bacterial solution: The concentration is 2.9×10 9 -4.5×10 9 cfu / ml of Bacillus megaterium CICC24484 (purchased from Beijing Yuwei Technology Co., Ltd., catalog number CICC 24484) and a concentration of 2.5×10 9-4.0×10 9 cfu / ml of Aspergillus oryzae CICC2066 (purchased from Shanghai Fuxiang Biotechnology Co., Ltd., catalog number: CICC2006),

[0006] S3, taking 30-40g of the crushed Platycodon grandiflorum and 100-110g of the Rhizoma Imperatae in the Chinese medicine prescription of step S1, mixing to obtain a mixture, adding Bacillus megaterium CICC 24484 bacterial solution and Aspergillus oryzae CICC2066 bacterial solution to the mixture in a mass ratio of 1:0.3-0.7:0.3-0.7, mixing and placing in a cylinder for aerobic fermentation at a temperature of 35-37° C. for 6-12 hours; after the fermentation is completed, adding 3% hydrogen peroxide to inactivate Bacillus megaterium CICC 24484 and Aspergillus oryzae CICC2066, and the obtained fermented product is used for standby;

[0007] S4, preparation of Chinese medicine liquid: take 20-30g of gypsum in the prescription, add 10-12 times the mass of gypsum water, boil for 30min, add 30-35g of Indigo, 60-70g of Radix Lycii, 60-65g of Radix Scutellariae, 100-110g of Radix Stemonae and 60-70g of Radix Dahuriane crushed in step S1, add 10-13 times the total mass of crushed Chinese medicine water, add the fermented product obtained in step S3, boil for 40 minutes, filter, collect the filtrate for standby use; add 8-10 times the mass of water to the obtained residue, boil for 30 minutes, filter, and the filtrate is standby use; add 6-8 times the mass of water to the obtained residue, boil for 30 minutes, filter, and the filtrate is standby use; Filter, discard the residue, combine the filtrate with the above two results, heat and concentrate at 60°C, and concentrate to a relative density of 1.1-1.2; put it to room temperature, add 95% ethanol, add it to the medicinal liquid until the alcohol content is 70%, seal it, keep the temperature at 16°C, let it stand for 48 hours for alcohol precipitation, take the supernatant and filter it, and the filtrate is ready for use; take 30-40g of the raw ephedra crushed in step S1, add 70% ethanol with a mass of 10-13 times of the raw ephedra, reflux extract at 80-85°C for 30-50min, cycle 3 times, discard the residue, combine the filtrate obtained from the 3 cycles, and combine it with the above alcohol precipitation liquid, recover the alcohol, and concentrate it until there is no alcohol taste, and the obtained Chinese medicine liquid is ready for use;

[0008] S5, matrix preparation: 15-30g of glyceryl monostearate and 15-30g of paraffin are placed in an evaporating dish, heated and melted in a water bath, and then 70-80g of liquid paraffin (heavy), 15-20g of white vaseline, and 1-2g of Span 80 are added, and the temperature is maintained at 80°C after complete melting; then 1-2g of OP emulsifier and 0.6-1.2g of chlorocresol are dissolved in 50-100g of aqueous solution, heated to 80°C, and then added to the above-mentioned oil phase solution maintained at 80°C, while continuously stirring in the forward direction, until it solidifies into a milky white semi-solid state to obtain the ointment matrix;

[0009] S6. Mix the ointment base obtained in step S5 with the traditional Chinese medicine liquid in a mass ratio of 3:1, add glycerol to make the total weight of the ointment 1 kg, place it in a high-pressure homogenizer, homogenize for 60 minutes at a flow rate of 0.1 T / H and a pressure of 25 mPa, and bottle it to obtain a traditional Chinese medicine ointment that can clear away heat, relieve cough and reduce phlegm, wherein the amount of glycerol added is 5-8% of the traditional Chinese medicine ointment.

[0010] Preferably: the Chinese medicine prescription in step S1 of the present invention is 30g of Indigo Naturalis, 60g of Lycium bark, 20g of Gypsum, 60g of Scutellaria baicalensis, 100g of Stemona, 60g of Radix Jasminoides, 30g of Ephedra sinica, 30g of Platycodon grandiflorum, and 100g of Imperatae Rhizoma.

[0011] Preferably, the auxiliary materials in step S1 of the present invention are 15.0 g of glyceryl monostearate, 15.0 g of paraffin, 70.0 g of liquid paraffin (heavy), 15.0 g of white vaseline, 801 g of Span, 1 g of OP emulsifier, 0.6 g of chlorocresol, and 50.0 g of distilled water.

[0012] Preferably, the concentration of the Bacillus megaterium CICC 24484 bacterial solution in step S2 of the present invention is 3.6×10 9 cfu / ml.

[0013] Preferably, the concentration of Aspergillus oryzae CICC2066 in step S2 of the present invention is 4.0×10 9 cfu / ml.

[0014] Preferably, in step S4 of the present invention, the medicinal liquid decocted three times is concentrated to a relative density of 1.2.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. The present invention uses:

[0017] Indigo: Contains ingredients such as indigo and indigo red, which has the effects of clearing heat and detoxifying, cooling blood and stopping bleeding.

[0018] Rehmannia root bark: It mainly contains phenolic compounds, such as rehmannia root, which has the effects of clearing heat and cooling blood, promoting body fluid and quenching thirst.

[0019] Gypsum: The main ingredient is calcium sulfate, which has the effects of clearing away heat and purging fire, promoting salivation and quenching thirst.

[0020] Scutellaria baicalensis: Contains ingredients such as baicalin and baicalein, which have the effects of clearing away heat and dampness, purging fire and detoxifying, and relieving cough and reducing phlegm.

[0021] Stemona: Contains ingredients such as stemonae and stemonasapogenins, which have the effects of moistening the lungs, relieving cough and killing insects.

[0022] Gardenia: Contains volatile oils, jasmine and other ingredients, which have the effects of clearing heat and resolving phlegm, relieving cough and relieving asthma.

[0023] Raw ephedra: contains ingredients such as ephedrine and pseudoephedrine, and has the effects of inducing sweating, relieving exterior symptoms, and clearing the lungs and relieving asthma.

[0024] Platycodon: Contains platycodon saponins, platycodic acid and other ingredients, which have the effects of clearing the lungs and relieving sore throat, resolving phlegm and relieving cough.

[0025] Imperata root: Contains ingredients such as imperatain and imperata glycoside, which have the effects of clearing away heat, promoting body fluid, and promoting diuresis and relieving stranguria.

[0026] 2. The present invention uses Bacillus megaterium CICC 24484 and Aspergillus oryzae CICC2066 to carry out synergistic fermentation on Platycodon grandiflorum and Imperata cylindrica, which is more conducive to the exertion of drug efficacy.

[0027] 3. The ointment obtained by the present invention not only has the effects of clearing away heat, relieving cough and reducing phlegm, but also has the unexpected effect of inhibiting asthma attacks. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a diagram of the cough latency period in the rat cough suppression experiment.

[0029] Figure 2 This is a graph showing the number of coughs in a rat cough suppression experiment.

[0030] Figure 3 The Chinese medicinal ointment prepared by the present invention Figure 1 .

[0031] Figure 4 The Chinese medicinal ointment prepared by the present invention Figure 2 . DETAILED DESCRIPTION

[0032] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0033] Example 1

[0034] This embodiment provides a traditional Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm, and the preparation process thereof comprises:

[0035] S1. Preparation of materials: Chinese medicine prescription: put 30g of Indigo Naturalis, 60g of Lycium bark, 20g of Gypsum, 60g of Scutellaria baicalensis, 100g of Stemona, 60g of Radix Daphne, 30g of Ephedra sinica, 30g of Platycodon grandiflorum and 100g of Imperatae cylindrica into a wall-breaking machine for grinding; auxiliary materials: 15g of glyceryl monostearate, 15g of paraffin, 70g of liquid paraffin, 15g of white vaseline, 1g of Span 80, 1g of OP emulsifier, 0.6g of chlorocresol and 50g of distilled water;

[0036] S2. Preparation of bacterial solution: The concentration is 3.6×10 9 cfu / ml of Bacillus megaterium CICC 24484 and a concentration of 4.0×10 9 cfu / ml of Aspergillus oryzae CICC2066 bacterial solution;

[0037] S3, taking 30g of Platycodon grandiflorum and 100g of Imperata cylindrica in the Chinese medicine prescription of step S1, mixing to obtain a mixture, adding Bacillus megaterium CICC 24484 bacterial solution and Aspergillus oryzae CICC2066 bacterial solution in a mass ratio of 1:0.3:0.3 to the mixture, mixing and loading into a cylinder for aerobic fermentation at a temperature of 37° C. for 6 hours; after the fermentation is completed, adding 3% hydrogen peroxide to inactivate Bacillus megaterium CICC 24484 and Aspergillus oryzae CICC2066, and the obtained fermentation product is used for standby;

[0038] S4, preparation of Chinese medicine liquid: take 20g of gypsum in the prescription, add 10 times the mass of gypsum water, boil for 30min, then add 30g of Indigo Naturalis, 60g of Radix Lycii, 60g of Radix Scutellariae, 100g of Radix Stemonae and 60g of Radix Glehniae crushed in step S1, add 10 times the total mass of crushed Chinese medicine water, then add the fermented product obtained in step S3 and boil for 40 minutes, filter, collect the filtrate for standby use; add 8 times the mass of water to the obtained residue, boil for 30 minutes, filter, and the filtrate is standby use; add 6 times the mass of water to the obtained residue, boil for 30 minutes, filter, and discard the residue. The filtrate is combined with the above two results, heated and concentrated at 60°C to a relative density of 1.1; cooled to room temperature, 95% ethanol is added until the alcohol content of the medicinal liquid is 70%, sealed, kept at 16°C, allowed to stand for 48 hours for alcohol precipitation, the supernatant is filtered, and the filtrate is used for standby; 30g of the raw ephedra crushed in step S1 is taken, 70% ethanol with a mass of 10 times that of the raw ephedra is added, refluxed and extracted at 80°C for 30min, the cycle is repeated 3 times, the residue is discarded, the filtrate obtained from the 3 cycles is combined, and the filtrate is combined with the above alcohol precipitation liquid, the alcohol is recovered, and concentrated until there is no alcohol taste, and the obtained Chinese medicine liquid is used for standby;

[0039] S5, matrix preparation: 15g of glyceryl monostearate and 15g of paraffin are placed in an evaporating dish, heated in a water bath to melt, and then 70g of liquid paraffin, 15g of white vaseline, and 801g of Span are added, and the temperature is maintained at 80°C after they are completely melted; then 1g of OP emulsifier and 0.6g of chlorocresol are dissolved in 50g of aqueous solution, heated to 80°C, and then added to the above-mentioned oil phase solution maintained at 80°C, while continuously stirring in the forward direction, until it solidifies into a milky white semi-solid state to obtain the ointment matrix;

[0040] S6. Mix the ointment base obtained in step S5 with the Chinese medicine liquid in a mass ratio of 3:1, add glycerol, so that the total weight of the ointment is 1 kg, place it in a high-pressure homogenizer, homogenize for 60 minutes at a flow rate of 0.1 T / H and a pressure of 25 mPa, and bottle it to obtain a Chinese medicine ointment that can clear away heat, relieve cough and reduce phlegm, wherein the amount of glycerol added is 8% of the Chinese medicine ointment. Chinese medicine ointment products such as Figure 3 and Figure 4 shown.

[0041] Comparative Example 1: Except that in step S3, Bacillus megaterium CICC 24484 bacterial solution is not used for aerobic fermentation, the remaining steps are the same as those in Example 1.

[0042] Comparative Example 2: Except that Aspergillus oryzae CICC2066 bacterial solution is not used for aerobic fermentation in step S3, the remaining steps are the same as those in Example 1.

[0043] Example 2

[0044] This embodiment provides a traditional Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm, and the preparation process thereof comprises:

[0045] S1. Preparation of materials: Chinese medicine prescription: 35g of Indigo Naturalis, 70g of Lycium bark, 30g of Gypsum, 65g of Scutellaria baicalensis, 110g of Stemona, 70g of Radix Daphne, 40g of Ephedra sinica, 40g of Platycodon grandiflorum and 110g of Imperatae cylindrica were put into a wall-breaking machine for crushing; auxiliary materials: 15g of glyceryl monostearate, 15g of paraffin, 70g of liquid paraffin, 15g of white vaseline, 801g of Span, 2g of OP emulsifier, 1.2g of chlorocresol and 100g of distilled water;

[0046] S2. Preparation of bacterial solution: The concentration is 2.9×10 9 cfu / ml of Bacillus megaterium CICC 24484 and a concentration of 2.5×10 9 cfu / ml of Aspergillus oryzae CICC2066 bacterial solution;

[0047] S3, taking 40g of Platycodon grandiflorum and 110g of Imperata cylindrica in the Chinese medicine prescription of step S1, mixing to obtain a mixture, adding Bacillus megaterium CICC 24484 bacterial solution and Aspergillus oryzae CICC2066 bacterial solution in a mass ratio of 1:0.7:0.7 to the mixture, mixing and loading into a cylinder for aerobic fermentation at a temperature of 37° C. for 12 hours; after the fermentation is completed, adding 3% hydrogen peroxide to inactivate Bacillus megaterium CICC 24484 and Aspergillus oryzae CICC2066, and the obtained fermentation product is used for standby;

[0048] S4, preparation of Chinese medicine liquid: take 30g of gypsum in the prescription, add water 12 times the mass of gypsum, boil for 30min, add 35g of Indigo, 70g of Radix Lycii, 65g of Radix Scutellariae, 110g of Radix Stemonae and 70g of Radix Glehniae crushed in step S1, add water 13 times the total mass of crushed Chinese medicine, add the fermented product obtained in step S3, boil for 40 minutes, filter, collect the filtrate for standby use; add 10 times the mass of water to the obtained residue, boil for 30 minutes, filter, and the filtrate is standby use; add 8 times the mass of water to the obtained residue, boil for 30 minutes, filter, and discard the residue , the filtrate is combined with the above two results, heated and concentrated at 60°C, and concentrated to a relative density of 1.2; put it to room temperature, add 95% ethanol, add it to the medicinal liquid until the alcohol content is 70%, seal it, keep the temperature at 16°C, let it stand for 48 hours for alcohol precipitation, take the supernatant and filter it, and the filtrate is used for standby; take 40g of the raw ephedra crushed in step S1, add 70% ethanol with a mass of 13 times that of the raw ephedra, reflux extract at 80°C for 50min, cycle 3 times, discard the residue, combine the filtrates obtained from the 3 cycles, and combine them with the above alcohol precipitation liquid, recover the alcohol, and concentrate it until there is no alcohol taste, and the obtained Chinese medicine liquid is used for standby;

[0049] S5, matrix preparation: 15g of glyceryl monostearate and 15g of paraffin are placed in an evaporating dish, heated in a water bath to melt, and then 70g of liquid paraffin, 15g of white vaseline, and 801g of Span are added, and the temperature is maintained at 80°C after they are completely melted; then 2g of OP emulsifier and 1.2g of chlorocresol are dissolved in 100g of aqueous solution, heated to 80°C, and then added to the above-mentioned oil phase solution maintained at 80°C, while continuously stirring in the forward direction, until it solidifies into a milky white semi-solid state to obtain the ointment matrix;

[0050] S6. Mix the ointment base obtained in step S5 with the traditional Chinese medicine liquid in a mass ratio of 3:1, add glycerol to make the total weight of the ointment 1 kg, place it in a high-pressure homogenizer, homogenize for 60 minutes at a flow rate of 0.1 T / H and a pressure of 25 mPa, and bottle it to obtain a traditional Chinese medicine ointment that can clear away heat, relieve cough and reduce phlegm, wherein the amount of glycerol added is 8% of the traditional Chinese medicine ointment.

[0051] Comparative Example 3: Except that Bacillus megaterium CICC 24484 in step S3 was replaced by Bacillus megaterium CICC 10055 (purchased from Beijing Yuwei Technology Co., Ltd., product number: CICC 10055) for aerobic fermentation, the remaining steps were the same as those in Example 2.

[0052] Comparative Example 4: Except that Aspergillus oryzae CICC2066 in step S3 is replaced by Aspergillus oryzae CICC2080 (purchased from Shanghai Xuanke Biotechnology Co., Ltd., product number: XK-SH-6221), the remaining steps are the same as those in Example 2.

[0053] Example 3

[0054] This embodiment provides a traditional Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm, and the preparation process thereof comprises:

[0055] S1. Preparation of materials: Chinese medicine prescription: 33g of Indigo Naturalis, 65g of Lycium bark, 25g of Gypsum, 64g of Scutellaria baicalensis, 105g of Stemona, 65g of Radix Daphne, 35g of Ephedra sinica, 35g of Platycodon grandiflorum and 105g of Imperatae cylindrica were put into a wall-breaking machine for crushing; auxiliary materials: 15g of glyceryl monostearate, 15g of paraffin, 70g of liquid paraffin, 15g of white vaseline, 1g of Span 80, 1g of OP emulsifier, 0.6g of chlorocresol and 50g of distilled water;

[0056] S2. Preparation of bacterial solution: The concentration is 4.5×10 9 cfu / ml of Bacillus megaterium CICC 24484 and a concentration of 3.8×10 9 cfu / ml of Aspergillus oryzae CICC2066 bacterial solution;

[0057] S3, taking 35g of Platycodon grandiflorum and 105g of Imperata cylindrica in the Chinese medicine prescription of step S1, mixing to obtain a mixture, adding Bacillus megaterium CICC 24484 bacterial solution and Aspergillus oryzae CICC2066 bacterial solution to the mixture in a mass ratio of 1:0.6:0.7, mixing and loading into a cylinder for aerobic fermentation at a temperature of 37° C. for 8 hours; after the fermentation is completed, adding 3% hydrogen peroxide to inactivate Bacillus megaterium CICC 24484 and Aspergillus oryzae CICC2066, and the obtained fermentation product is used for standby;

[0058] S4, preparation of Chinese medicine liquid: take 25g of gypsum in the prescription, add water 11 times the mass of gypsum, boil for 30min, add 33g of Indigo, 65g of Radix Lycii, 64g of Radix Scutellariae, 105g of Radix Stemonae and 65g of Radix Glehniae crushed in step S1, add water 12 times the total mass of crushed Chinese medicine, add the fermented product obtained in step S3, boil for 40 minutes, filter, collect the filtrate for standby use; add 9 times the mass of water to the obtained residue, boil for 30 minutes, filter, and the filtrate is standby use; add 7 times the mass of water to the obtained residue, boil for 30 minutes, filter, and discard the residue. The filtrate is combined with the above two results, heated and concentrated at 60°C to a relative density of 1.2; cooled to room temperature, 95% ethanol is added until the alcohol content of the medicinal liquid is 70%, sealed, kept at 16°C, allowed to stand for 48 hours for alcohol precipitation, the supernatant is filtered, and the filtrate is used for standby; 35g of the raw ephedra crushed in step S1 is taken, 70% ethanol with a mass of 11 times that of the raw ephedra is added, refluxed and extracted at 85°C for 40min, the cycle is repeated 3 times, the medicinal residue is discarded, the filtrate obtained from the 3 cycles is combined, and the filtrate is combined with the above alcohol precipitation liquid, the alcohol is recovered, and concentrated until there is no alcohol taste, and the obtained Chinese medicine liquid is used for standby;

[0059] S5, matrix preparation: 15g of glyceryl monostearate and 15g of paraffin are placed in an evaporating dish, heated in a water bath to melt, and then 70g of liquid paraffin, 15g of white vaseline, and 801g of Span are added, and the temperature is maintained at 80°C after they are completely melted; then 1g of OP emulsifier and 0.6g of chlorocresol are dissolved in 50g of aqueous solution, heated to 80°C, and then added to the above-mentioned oil phase solution maintained at 80°C, while continuously stirring in the forward direction, until it solidifies into a milky white semi-solid state to obtain the ointment matrix;

[0060] S6. Mix the ointment base obtained in step S5 with the traditional Chinese medicine liquid in a mass ratio of 3:1, add glycerol to make the total weight of the ointment 1 kg, place it in a high-pressure homogenizer, homogenize for 60 minutes at a flow rate of 0.1 T / H and a pressure of 25 mPa, and bottle it to obtain a traditional Chinese medicine ointment that can clear away heat, relieve cough and reduce phlegm, wherein the amount of glycerol added is 8% of the traditional Chinese medicine ointment.

[0061] Comparative Example 5: Except that decoction is performed directly without using bacterial liquid fermentation in step S3, the remaining steps are the same as those in Example 3.

[0062] Comparative Example 6: Except that the raw ephedra in step S4 is not subjected to reflux extraction at 85° C. using 70% ethanol but is directly decocted in water, the remaining steps are the same as those in Example 3.

[0063] Example 4

[0064] Animal experiment: Rat cough test

[0065] 1. Preparation of respiratory syncytial virus (RSV) suspension: Hep-2 cells (purchased from Shanghai Bo Ke Biotechnology Co., Ltd.) were cultured in a 37°C cell culture incubator (CO2 concentration of 5%). When the bottom of the culture bottle was full of cells, 10ul RSV virus Long strain (purchased from Qingdao Henderson Biotechnology Co., Ltd.) was added to 2ml cell maintenance solution. The maintenance solution containing RSV was inoculated into the Hep-2 cell culture bottle, and the culture bottle was placed in a carbon dioxide incubator to adsorb the virus for 1 hour. Then 2ml of cell maintenance solution was continued to be added to the culture bottle, and the cell culture bottle containing the virus was placed in a carbon dioxide incubator. Daily observation under an inverted microscope showed that the cells gradually became round or fell off and died after being infected by the virus. When the cell pathological rate reached more than 90%, the culture solution was frozen in a -20°C refrigerator, then taken out, dissolved at room temperature, and after repeated freezing and thawing 3 times, the cells were centrifuged for 5 minutes (4°C / 1500rpm), and the virus supernatant containing RSV was sucked out and placed in an EP tube at -80°C for standby use.

[0066] 2. Rat modeling and grouping and drug administration: Healthy adult SPF-grade SD rats (purchased from Beijing Huafukang Biotechnology Co., Ltd.) were selected, weighing 200±25g, half male and half female, for a total of 130 rats. The rats were kept in an animal room at room temperature and a humidity of about 55%, simulating the biological cycle of animals with alternating day and night (12h / 12h), and were adaptively fed with a sterile diet for 1 week. During the experiment, they were free to drink water and eat. Then 10 rats were randomly selected to form a normal group and fed in a separate cage. The remaining 110 rats were used for modeling. Ether anesthesia was used. The rats were placed in a glass jar filled with ether gas for light anesthesia. After light anesthesia, the model group was slowly dripped with RSV Long strain virus liquid (titer of 5×10 4 TCID50 / 0.1ml), 100uL / rat, for 3 consecutive days. The first virus inoculation day was defined as the experimental day 0. On the 13th day, the rats were placed in a 5L closed nebulizer box, and capsaicin solution (concentration of 1×10 -4 mol / L) was slowly input into the atomization box to induce rat cough. When the rat had abdominal muscle contraction or chest shrinkage, and there was a cough when the mouth was wide open, it was considered that the modeling was successful. Administration was started on the 14th day. Before administration, the normal group and modeling mice were shaved at the throat and back. 100 rats completed by modeling were selected and randomly divided into a model group, an embodiment group (embodiment 1-3), and a comparative example group (comparative example 1-6) for administration. Each group had 10 rats. The normal group and the model group were smeared with the ointment base prepared in step S5 at the throat and back. The embodiment group was smeared with the Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm prepared by embodiment 1-3 at the throat and back respectively. The comparative example group was smeared with the Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm prepared by comparative example 1-6 at the throat and back respectively. The throat and back were both smeared with 0.1g / cm 2 / d was applied. The drug was administered once a day for 14 days. The status of the rats in each group was observed and recorded every day.

[0067] 3. Cough suppression experiment

[0068] One hour after the last administration, rats in all groups except the normal control group were placed in a 5L closed nebulizer and capsaicin solution (concentration of 1×10 -4 mol / L) was slowly introduced into the atomization box to fill the atomization box with capsaicin smoke. The rats' cough latency and frequency were recorded. The observation was conducted for 5 minutes. The results are shown in Table 1. Figure 1 and Figure 2 shown.

[0069] 4. Expectorant experiment

[0070] 0.5h after the last administration, 0.5% phenol red saline solution was injected intraperitoneally at 0.01mL / g. 30min after the injection, rats were killed by dislocation of the neck, the muscle tissue around the bronchi was stripped, and a section of trachea from the thyroid cartilage to the tracheal branch was cut and placed in an EP tube filled with 2mL of saline. After vortexing for 5min, ultrasonic cleaning was performed for 20min, and then 1mL of 5% NaHCO3 solution was added dropwise, and the tube was placed in a centrifuge at 2500r / min for 10min. The OD value of each group was measured at 546nm with 5% NaHCO3 as the standard solution using a visible spectrophotometer, and a standard curve was made with 5% phenol red solution. The phenol red content was calculated through the standard curve. The results are shown in Table 2.

[0071] Table 1

[0072]

[0073] Table 2

[0074]

[0075]

[0076] The present invention prepares a Chinese medicine ointment by synergistically fermenting platycodon grandiflorum and cogongrass root with Bacillus megaterium CICC 24484 and Aspergillus oryzae CICC2066, decocting the platycodon grandiflorum, Chinese wolfberry bark, gypsum, scutellaria baicalensis, stemonae and jasmine after fermentation, performing alcohol precipitation after decoction, and mixing the mixture with an alcohol-extracted raw ephedra extract to obtain the Chinese medicine ointment, which has excellent heat-clearing, cough-relieving and phlegm-resolving effects. According to Table 1, Table 2 and Figure 1-2The test results of the embodiment can prove it. According to the results of comparative examples 1 and 2, it can be seen that when a single bacterial liquid is used for fermentation, the curative effect is much lower than the curative effect of the synergistic fermentation of bacillus megaterium CICC 24484 and aspergillus oryzae CICC2066, so that the cough latency of rats is shortened, the number of coughs increases, and the amount of expectoration is reduced. It is worth noting that the curative effect of comparative example 5, which does not use bacterial liquid for fermentation, is more significantly reduced, which shows that aerobic synergistic fermentation using bacillus megaterium CICC 24484 and aspergillus oryzae CICC2066 is more conducive to the performance of drug effect. According to the results of comparative examples 3 and 4, it can be obtained that bacillus megaterium CICC 24484 and aspergillus oryzae CICC2066 have specificity for the fermentation of platycodon grandiflorum and cogongrass root. It can also be observed that using ethanol to heat and reflux extraction of raw ephedra can also improve the curative effect of ointment.

[0077] Relieve asthma in rats:

[0078] Fine particulate matter: PM 2.5 ; Ovalbumin: OVA

[0079] Preparation of OVA and PM2.5 mixed solution: weigh 3 mg of OVA, add physiological saline, and prepare an OVA solution with a concentration of 1 mg / ml, which is the OVA stock solution. Use a pipette to take 1 ml of OVA stock solution, add 11.5 ml of physiological saline, and prepare an OVA solution with a concentration of 80 μg / ml; weigh 15 mg of PM2.5, add 15 ml of physiological saline to prepare a 1 mg / ml PM2.5 solution, and mix by ultrasonic. Take 12.5 ml of PM2.5 solution and 12.5 ml of OVA solution and mix them to obtain an OVA+PM2.5 mixed solution. Finally, divide the solution into portions according to the amount used for each instillation and store at -20°C for future use.

[0080] Rat modeling and drug administration: Healthy adult SPF SD rats (purchased from Beijing Huafukang Biotechnology Co., Ltd.) were selected, weighing 200±25g, half male and half female, a total of 110 rats, and the rats were raised in the animal room at room temperature and humidity of about 55%, simulating the biological cycle of animals, alternating day and night (12h / 12h), and fed with sterile diet for 1 week. During the experiment, they were free to drink water and eat. Before the experiment, all rats were shaved at the throat and back, and then the 110 rats were randomly divided into 11 groups, namely a normal group, a model group, an embodiment group (embodiments 1-3), and a comparative example group (comparative example 1-6), with 10 rats in each group. The model group, the embodiment group and the comparative example group were instilled with OVA and PM2.5 poisoning by tracheal instillation, and the normal group was instilled with normal saline. Both were administered at 0.1 ml / 100 g, and the intervention was performed once every 2 weeks, for a total of 4 interventions; after successful modeling, the embodiment group and the comparative example group were smeared with the Chinese medicine ointment prepared by the embodiment and the comparative example group on the throat and back 30 minutes before tracheal instillation, and the ointment base prepared in step S5 was applied to the throat and back of the normal group and the model group, and the throat and back were both smeared at 0.1 g / cm 2 The samples were collected 24 hours after the last instillation. The rats were deeply anesthetized with 4% isoflurane, and the heart blood and bronchoalveolar lavage fluid were collected.

[0081] The collection methods of cardiac blood and bronchoalveolar lavage fluid are:

[0082] Collection of cardiac blood serum: Place the rat in supine position with its limbs fixed on the operating table, and wipe the chest and abdomen with alcohol cotton balls for disinfection. First make a small cut in the epidermis, and then cut it in a "V" shape to expose the chest and abdomen. Then use forceps to lift the xiphoid process and cut the diaphragm. While fully exposing the chest cavity along the incision, avoid injuring the heart and lungs. Use a 1ml syringe to collect cardiac blood. Let it stand at room temperature for more than 2 hours and centrifuge (3500rpm, 5min). After centrifugation, collect the serum in an EP tube and mark it. Used to detect the levels of OVA-IgE and OVA-IgG1 in the serum.

[0083] Bronchoalveolar lavage fluid (BALF) collection: Cut the rat's neck skin, and separate the neck muscles, fascia and other tissues in turn. Completely expose the trachea in the neck and insert a surgical thread under the trachea. Then cut a small hole in the trachea, gently insert a retention needle, and fix it with a surgical thread. Use a 1ml syringe to extract 0.8ml of 37℃ normal saline, connect it to the retention needle to lavage the lungs, repeat the operation twice, and ensure a recovery rate of >80%. Finally, centrifuge the collected BALF at 4℃, 1500rpm for 10min, and collect the supernatant. Used to detect the level of cytokines in BALF.

[0084] Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of cytokines (TNF-α, IL-6, and IL-1β) in rat BALF samples and OVA-IgE and OVA-IgG1 in serum. The results are shown in Tables 3 and 4.

[0085] In this experiment, the levels of OVA-IgE and OVA-IgG1 in rat serum were detected by ELISA experiment. It can be seen from Table 3 that compared with the normal group, the levels of OVA-IgE and OVA-IgG1 in the serum of the rats in the model group were increased; compared with the model group, the levels of OVA-IgE and OVA-IgG1 in the serum of the rats in the example group treated with the heat-clearing, cough-relieving and phlegm-relieving ointment were significantly reduced. These results suggest that hawthorn acid may exert an anti-inflammatory effect by reducing the level of OVA-specific antibodies in serum. The results of the comparative group show that Platycodon grandiflorum and Imperata cylindrica root can show excellent asthma-inhibiting effects after being synergistically fermented by Bacillus megaterium CICC 24484 and Aspergillus oryzae CICC2066.

[0086] Table 3

[0087]

[0088] IL-6 is usually considered as a biomarker of persistent inflammation together with TNF-α and IL-1β. The experimental results of this study showed (Table 4) that the levels of IL-6, TNF-α and IL-1β in the BALF of rats in the model group were significantly increased. After treatment with the heat-clearing, cough-relieving and phlegm-removing Chinese medicine ointment, the levels of these pro-inflammatory factors were significantly reduced. This further confirmed that the heat-clearing, cough-relieving and phlegm-removing Chinese medicine ointment may play an anti-inflammatory role in asthma by reducing the levels of inflammatory cytokines in rat BALF.

[0089] Table 4

[0090]

[0091] Clinical cases:

[0092] Case 1, Wu * Chen, male, 4 years old, date of consultation: 2024.4.01, location of consultation: Beijing Kyoto Children's Hospital.

[0093] Chief complaint: Cough (Second visit)

[0094] Current medical history: The child still has cough, paroxysmal and continuous coughing, sputum is difficult to cough up, coughs more at night, no fever, runny nose, no hoarseness, wheezing, vomiting or diarrhea. Since the onset of the disease, the child has been in good spirits, has a good diet and sleep, and has normal urination and defecation.

[0095] History of medication: Eliminating phlegm and relieving cough

[0096] Past medical history: The patient is in good health and denies any history of infectious diseases or close contact with infectious diseases.

[0097] Physical examination: body temperature 36.1℃, pain assessment: none, high risk of falling: yes, intervention measures for falls: education, family members accompanying; nutritional screening: no abnormalities; psychological screening: no abnormalities; functional rehabilitation: not required; special population: no. Clear consciousness, good spirits, stable breathing. No rash or bleeding spots, no palpable superficial lymph nodes. Congestion, no herpes, bilateral tonsils enlarged to grade I, no purulent secretions. Soft neck, coarse breath sounds in both lungs, no dry or wet rales. Strong and regular heart sounds, no murmurs in the auscultation area of ​​each valve. Soft abdomen without tenderness, normal bowel sounds. No abnormalities in the nervous system. Red tip of tongue, white and greasy fur, fine and rapid pulse. Auxiliary examination results: 2024-03-30 clinical examination report: C-reactive protein <0.5mg / L, white blood cell count 5.96*109 / L, neutrophil count 2.45*10~9 / L, lymphocyte count 3.02*10^9 / L, monocyte count 0.40*10~9 / L, eosinophil count 0.05*10~9 / L, basophil count 0.04*10^9 / L, neutrophil% 41.10%, lymphocyte% 50.70%, monocyte% 6.70%, eosinophil ratio 0.80%, basophil ratio 0.70%, red blood cell 4.6*1 0~12 / L, hemoglobin 134g / L, hematocrit 40.40%, mean corpuscular volume 88.2fL, mean hemoglobin content 29.3Pg, mean hemoglobin concentration 332g / L, red blood cell volume distribution width -SD40.00fL, red blood cell volume distribution width -CV12.70%, platelet count 342*10^9 / L, platelet volume distribution width 9.1fL, mean platelet volume 9.1fL, large platelet ratio 16.50%, platelet ratio 0.31%, Mycoplasma pneumoniae IgM antibody weakly positive (±), amyloid protein A1.2mg / L.

[0098] Preliminary diagnosis (or diagnosis): 1 Bronchitis 2 Rhinitis 3 Children's cough 4 Lung and stomach heat syndrome

[0099] Treatment process: Take oral medication for symptomatic treatment as prescribed by the doctor and observe the efficacy.

[0100] Educational guidance: pay attention to rest, eat light food, drink plenty of water. Strengthen airway management, pat the back frequently to promote sputum discharge

[0101] Health education effectiveness evaluation: known

[0102] Follow-up treatment instructions: Observe the condition closely and follow up if symptoms worsen or the condition changes.

[0103] Disposal:

[0104] Treatment [A] Tuina therapy for children's cough / [A] Chinese medicine ointment massage / [A] Acupoint application therapy / [A] Intradermal acupuncture therapy /

[0105] Third visit: 2024.4.05, the child's cough was relieved after massage, sputum was easy to cough up, sputum symptoms improved, night coughs decreased, no fever, runny nose, no hoarseness, wheezing, vomiting or diarrhea. Since the onset of the disease, the child has been in good spirits, has a good diet and sleep, and has normal urination and defecation.

[0106] Case 2, Zhang *Fan, female, 9 years old, consultation time: 2024.4.05, consultation location: Beijing Kyoto Children's Hospital.

[0107] Chief complaint: Cough

[0108] Current medical history: The child's cough is less severe than before, with sputum that is difficult to cough up, paroxysmal cough, more coughing after meals, shortness of breath, no nasal congestion, runny nose, no fever, occasional abdominal pain, and good spirits since the onset of the disease, good diet and sleep, and normal urination and defecation. The pertussis test was negative during the course of the disease.

[0109] Current medication history: sulfonamides, traditional Chinese medicine

[0110] Past medical history: The patient is in good health and denies any history of infectious diseases or close contact with infectious diseases.

[0111] Physical examination: body temperature 36.5℃, pain assessment: none, high risk of falling: yes, intervention measures for falling: suitable for teaching, family members accompanying; nutritional screening: no abnormalities; psychological examination: no abnormalities, functional rehabilitation: not required; special population: no. Clear consciousness, good spirits, stable breathing. No rash or bleeding spots, no superficial lymph nodes were palpable. Pharyngeal congestion, scattered bleeding spots on the face (considered to be related to vomiting), no herpes. Soft, coarse breath sounds in both lungs, no dry or wet rales. Strong and regular heart sounds, no murmurs were heard in the auscultation area of ​​each valve. Soft abdomen without tenderness, normal bowel sounds. No abnormalities were found in the nervous system. Red tongue, thick and greasy white fur, slippery and rapid pulse.

[0112] Auxiliary examination results: Not performed today.

[0113] Preliminary diagnosis (or diagnosis): 1. Bronchitis 2. Children's cough 3. Lung and stomach heat syndrome

[0114] Treatment process: Massage, acupoint application therapy

[0115] Condition at discharge (physical examination and outcome):

[0116] Educational guidance: Take rest, eat light food, drink plenty of water, and prevent cross infection.

[0117] Evaluation of health education effectiveness: known.

[0118] Follow-up treatment instructions: Closely observe the condition, follow up if symptoms worsen or the condition changes. 3-day outpatient follow-up.

[0119] Disposal:

[0120] Treatment 1 [A] Tuina treatment for children's cough / 2 [A] Chinese medicine ointment massage / 3 [A] Intradermal acupuncture treatment / 4 [A] Cupping treatment /

[0121] Third visit: April 9, 2024. After the child cooperated with massage, the sputum decreased, the cough was relieved, the cough after meals became less, the shortness of breath was relieved, there was no nasal congestion, runny nose, no fever, abdominal pain disappeared, diet and sleep were good, and urination and defecation were normal.

[0122] Case 3, Zhu * Yi, female, 1 year and 1 month old, date of consultation: 2024.4.23, location of consultation: Beijing Kyoto Children's Hospital.

[0123] Current medical history: Cough appeared 2 months ago, and the symptoms were alleviated after treatment. Now cough more often at night and in the morning, no nasal congestion, runny nose, no fever, no wheezing, general condition is good, diet and sleep are normal, sweating at night, urination and defecation are normal. There is a history of allergic rhinitis and allergic cough.

[0124] Current medication history: None

[0125] Past medical history: The patient was in good health, denied any history of infectious diseases or close contact with infectious diseases, denied any history of asthma, and denied any history of foreign body inhalation.

[0126] Physical examination: body temperature 36.5℃, pain assessment: none, high risk of falling: yes, intervention measures for falling: suitable for teaching, family members accompanying. Conscious, mentally OK, stable breathing, no nose, no "three-depression sign", no rash, no bleeding spots, superficial lymph nodes not touched, no congestion of conjunctiva, no congestion of pharynx, no enlargement of bilateral tonsils, no blisters, soft neck, symmetrical breath sounds of both lungs, slightly coarse breath sounds, no dry or wet rales, strong heart sounds, no murmurs, regular rhythm, soft abdomen, no tenderness, no rebound pain, warm extremities. Red tip of tongue, white and greasy fur. Purple fingerprints. Auxiliary examination results: not checked today

[0127] Initial diagnosis (or diagnosis): 1 respiratory tract infection 2 cough 3 food stagnation syndrome Treatment: massage, plaster therapy

[0128] Educational guidance: Eat a light diet, avoid cold and greasy foods, avoid allergenic foods and contact with allergens, get enough rest, avoid catching cold, and prevent cross infection.

[0129] Health education effectiveness evaluation: known

[0130] Follow-up treatment instructions: Return for follow-up in 5 days

[0131] Disposal:

[0132] Treatment [A] Tuina treatment for children's cough / [A] Chinese medicine ointment massage / [A] Acupoint application treatment / [A] Tuina treatment for children's cough / [A] Chinese medicine ointment massage / [A] Acupoint application treatment /

[0133] Follow-up visit: 2024.4.26. After the child cooperated with the massage, the night cough disappeared, the morning cough was reduced, there was no nasal congestion, runny nose, no fever, no wheezing, the general condition was good, the diet and sleep were normal, the night sweats were excessive, and the urination and defecation were normal.

[0134] The above-mentioned embodiments only express the specific implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the protection scope of the present invention. It should be pointed out that, for those skilled in the art, without departing from the concept of the present invention, any other changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principle of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A preparation process of a Chinese medicinal ointment for clearing away heat, relieving cough and reducing phlegm, which specifically comprises the following steps: S1. Preparation of materials: Chinese medicine prescription: put 30-35g of Indigo Naturalis, 60-70g of Radix Lycii, 20-30g of Gypsum, 60-65g of Radix Scutellariae, 100-110g of Radix Stemonae, 60-70g of Radix Daphne, 30-40g of Ephedrae Herba, 30-40g of Platycodon Grandiflorum and 100-110g of Rhizoma Imperatae into a wall-breaking machine for grinding; auxiliary materials: 15-30g of glyceryl monostearate, 15-30g of paraffin, 70-80g of liquid paraffin (heavy), 15-20g of white vaseline, 1-2g of Span 80, 1-2g of OP emulsifier, 0.6-1.2g of chlorocresol and 50-100g of distilled water; S2. Preparation of bacterial solution: The concentration is 2.9×10 9 -4.5×10 9 cfu / ml of Bacillus megaterium CICC 24484 and a concentration of 2.5×10 9 -4.0×10 9 cfu / ml of Aspergillus oryzae CICC2066 bacterial solution; S3, taking 30-40g of the crushed Platycodon grandiflorum and 100-110g of the Rhizoma Imperatae in the Chinese medicine prescription of step S1, mixing to obtain a mixture, adding Bacillus megaterium CICC 24484 bacterial solution and Aspergillus oryzae CICC2066 bacterial solution to the mixture in a mass ratio of 1:0.3-0.7:0.3-0.7, mixing and placing in a cylinder for aerobic fermentation at a temperature of 35-37° C. for 6-12 hours; after the fermentation is completed, adding 3% hydrogen peroxide to inactivate Bacillus megaterium CICC 24484 and Aspergillus oryzae CICC2066, and the obtained fermented product is used for standby; S4, preparation of Chinese medicine liquid: take 20-30g of gypsum in the prescription, add 10-12 times the mass of gypsum water, boil for 30min, add 30-35g of Indigo, 60-70g of Radix Lycii, 60-65g of Radix Scutellariae, 100-110g of Radix Stemonae and 60-70g of Radix Dahuriane crushed in step S1, add 10-13 times the total mass of crushed Chinese medicine water, add the fermented product obtained in step S3, boil for 40 minutes, filter, collect the filtrate for standby use; add 8-10 times the mass of water to the obtained residue, boil for 30 minutes, filter, and the filtrate is standby use; add 6-8 times the mass of water to the obtained residue, boil for 30 minutes, filter, and the filtrate is standby use; Filter, discard the residue, combine the filtrate with the above two results, heat and concentrate at 60°C, and concentrate to a relative density of 1.1-1.2; put it to room temperature, add 95% ethanol, add it to the medicinal liquid until the alcohol content is 70%, seal it, keep the temperature at 16°C, let it stand for 48 hours for alcohol precipitation, take the supernatant and filter it, and the filtrate is ready for use; take 30-40g of the raw ephedra crushed in step S1, add 70% ethanol with a mass of 10-13 times of the raw ephedra, reflux extract at 80-90°C for 30-50min, cycle 3 times, discard the residue, combine the filtrate obtained from the 3 cycles, and combine it with the above alcohol precipitation liquid, recover the alcohol, and concentrate it until there is no alcohol taste, and the obtained Chinese medicine liquid is ready for use; S5, matrix preparation: 15-30g of glyceryl monostearate and 15-30g of paraffin are placed in an evaporating dish, heated and melted in a water bath, and then 70-80g of liquid paraffin (heavy), 15-20g of white vaseline, and 1-2g of Span 80 are added, and the temperature is maintained at 80°C after complete melting; then 1-2g of OP emulsifier and 0.6-1.2g of chlorocresol are dissolved in 50-100g of aqueous solution, heated to 80°C, and then added to the above-mentioned oil phase solution maintained at 80°C, while continuously stirring in the forward direction, until it solidifies into a milky white semi-solid state to obtain the ointment matrix; S6. Mix the ointment base obtained in step S5 with the traditional Chinese medicine liquid in a mass ratio of 3:1, add glycerol to make the total weight of the ointment 1 kg, place it in a high-pressure homogenizer, homogenize for 60 minutes at a flow rate of 0.1 T / H and a pressure of 25 mPa, and bottle it to obtain a traditional Chinese medicine ointment that can clear away heat, relieve cough and reduce phlegm, wherein the amount of glycerol added is 5-8% of the traditional Chinese medicine ointment.

2. The preparation process of a traditional Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm according to claim 1, characterized in that: The Chinese medicine prescription in step S1 is 30g of Indigo Naturalis, 60g of Lycium bark, 20g of Gypsum, 60g of Scutellaria baicalensis, 100g of Stemona, 60g of Radix Gypsum, 30g of Ephedra sinica, 30g of Platycodon grandiflorum, and 100g of Imperata cylindrica.

3. The preparation process of a traditional Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm according to claim 2, characterized in that: The auxiliary materials in step S1 are 15.0 g of glyceryl monostearate, 15.0 g of paraffin, 70.0 g of liquid paraffin (heavy), 15.0 g of white vaseline, 801 g of Span, 1 g of OP emulsifier, 0.6 g of chlorocresol, and 50.0 g of distilled water.

4. The preparation process of a traditional Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm according to claim 3, characterized in that: The concentration of the Bacillus megaterium CICC 24484 bacterial solution in step S2 is 3.6×10 9 cfu / ml.

5. The preparation process of a traditional Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm according to claim 4, characterized in that: The concentration of Aspergillus oryzae CICC2066 in step S2 is 4.0×10 9 cfu / ml.

6. The preparation process of a traditional Chinese medicine ointment for clearing away heat, relieving cough and reducing phlegm according to claim 5, characterized in that: In the step S4, the medicinal liquid decocted three times is concentrated to a relative density of 1.

2.

7. A Chinese medicinal ointment obtained by the preparation process of a Chinese medicinal ointment for clearing away heat, relieving cough and reducing phlegm according to any one of claims 1 to 6.

Images