Full-automatic microorganism culture detection system

Abstract

The invention discloses a full-automatic microbial culture detection system, which comprises a pretreatment module, a culture module and an automatic counting calculation module, the pretreatment module comprises a conveying unit, a sample sending unit and a charging unit, the sample sending unit and the charging unit are used for respectively conveying a sample and a culture medium into a culture dish, and a shaking device is arranged at the tail end of the conveying unit. The culture module comprises a box body, a culture space is formed in the box body, a rail module is arranged in the box body, the conveying unit is used for conveying culture dishes to the rail module, a shooting unit is arranged at the tail end, corresponding to the rail module, in the box body, and automatic sample feeding and charging operation of the culture dishes is achieved through mutual cooperation of the multiple units. The culture dish is further transferred into a culture space for culture, and after culture is completed, shooting recognition, automatic bacterial colony number counting and detection result calculation are carried out through a shooting unit, so that full-automatic microorganism culture detection is realized.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism detection, and more specifically to a full-automatic microorganism cultivation detection system. Background Art

[0002] The total colony count refers to the total number of bacterial colonies grown per gram (per milliliter) of the sample under certain conditions (such as aerobic conditions, nutritional conditions, pH, culture temperature and time, etc.). It is mainly used to determine the degree of bacterial contamination and sanitary quality of food. It reflects whether the food meets the sanitary requirements during the production process, so as to make appropriate hygienic evaluation of the sample being tested. The total colony count, to a certain extent, indicates the quality of food hygiene.

[0003] Currently, the total colony count is determined by manually culturing samples at multiple dilution levels. Based on the estimation of the sample contamination status, 1 to 3 appropriate dilution gradients are selected, and 2 plates or paper slips are made for each dilution. After the cultivation is completed, the colony count is completed by manual counting. This method is very inefficient due to the large number of actual culture plates or paper slips, and the burden on experimenters is also heavy, so it needs to be improved. Summary of the invention

[0004] In view of the deficiencies in the prior art, the object of the present invention is to provide a fully automatic microbial culture and detection system to achieve fully automatic culture and detection of samples.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A fully automatic microbial culture detection system, comprising a pretreatment module, a culture module and an automatic counting and calculation module;

[0007] The pretreatment module comprises a conveying unit, a sample delivery unit and a feeding unit, wherein the sample delivery unit and the feeding unit are used to deliver the sample and the culture medium into the culture dish respectively, and a shaking device is provided at the end of the conveying unit;

[0008] The culture module comprises a box body, a culture space is formed in the box body, a track module is arranged in the box body, the conveying unit is used for conveying the culture dish to the track module, and a shooting unit is arranged in the box body at the end corresponding to the track module.

[0009] The automatic counting and calculation module is used to automatically count and calculate the photos taken by the shooting unit.

[0010] As a further improvement of the present invention, the conveying unit forms a receiving station, a sample delivery station and a feeding station along the conveying direction respectively, and the pretreatment module also includes a feeding device arranged corresponding to the receiving station, and the feeding device is used to convey the culture dish to the conveying unit. The feeding device includes a barrel, and the barrel is vertically arranged. A discharging mechanism is provided at the lower end of the barrel, and the discharging mechanism includes a driving cylinder and a pushing block. The output end of the driving cylinder is connected to the pushing block, and the driving cylinder extends out and pushes the culture dish to be transferred to the conveying unit through the pushing block.

[0011] As a further improvement of the present invention, the sample delivery unit and the feeding unit both include a storage tank body and a transfer mechanism. A transfer port is formed at the upper end of the storage tank body. The transfer mechanism includes a first cylinder slider, a telescopic cylinder and a transfer gun. The telescopic cylinder is connected to the first cylinder slider, and the transfer gun is connected to the output end of the telescopic cylinder. A transfer cavity is formed in the transfer gun and a liquid outlet is formed at the lower end. A control cylinder and a piston are provided in the transfer cavity. The control cylinder is connected to the piston and drives the piston to move in the transfer cavity to control the liquid outlet to absorb / discharge liquid.

[0012] As a further improvement of the present invention, the shaking device includes a vibration generator, and the vibration generator is connected to the conveying unit.

[0013] As a further improvement of the present invention, the track module includes a translation track and a longitudinal moving device, and the translation track and the longitudinal moving device are used to drive the culture dish to move horizontally and vertically respectively.

[0014] As a further improvement of the present invention, the translation track includes a plurality of track units arranged parallel to each other, and the driving directions of adjacent track units are opposite. The translation track also includes an arc-shaped guide section, and both ends of the guide section are respectively connected to one end of the two track units connected to each other.

[0015] As a further improvement of the present invention, the longitudinal moving device includes a second cylinder slider and a support plate, the second cylinder slider is arranged corresponding to the moving passage, the support plate and the second cylinder slider are fixedly arranged, a separation module is provided on the support plate, and the separation module is used to drive the culture dish to separate from the support plate and move in the direction of the translation track, the separation module includes a third cylinder slider and a toggle rod, the third cylinder slider is arranged on the support plate, and the toggle rod is connected to the third cylinder slider.

[0016] As a further improvement of the present invention, a temperature control unit and a humidity control unit are provided in the culture space.

[0017] As a further improvement of the present invention, the track unit includes a driving motor, a conveyor belt and a driven wheel, the output end of the driving motor is provided with a driving wheel, the conveyor belt is respectively wound around the driving wheel and the driven wheel, a support frame is provided corresponding to the conveyor belt in the culture space, the support frame is used to keep the conveyor belt supported, and limit blocks are arranged on the conveyor belt, and a accommodating space for accommodating culture dishes is formed between adjacent limit blocks.

[0018] As a further improvement of the present invention, an operation panel is provided on one side of the box.

[0019] As a further improvement of the present invention, an automatic counting module and a calculation module are arranged in the operation panel.

[0020] Beneficial effects of the present invention:

[0021] 1. Through the cooperation between multiple units, the automatic sample delivery and material addition operations of the culture dish are realized, and the culture dish is further transferred to the culture space for cultivation. After the cultivation is completed, the shooting unit is used to shoot and identify, and the counting and calculation of the picture is further realized through the automatic counting and calculation module, thereby realizing fully automated microbial culture detection;

[0022] 2. By setting up the shooting unit, the recognition speed of the colonies can be greatly improved through image recognition, thereby improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the overall installation of the present invention;

[0024] Figure 2 This is a schematic diagram of the installation of the shooting unit of the present invention;

[0025] Figure 3 It is a schematic diagram of the feeding device of the present invention;

[0026] Figure 4 It is a schematic diagram of the installation of the transfer mechanism and the storage tank of the present invention;

[0027] Figure 5 It is a cross-sectional schematic diagram of the cleaning unit of the present invention;

[0028] Figure 6 for Figure 1 A partial enlarged view;

[0029] Figure 7 This is a schematic cross-sectional view of the installation of the track unit of the present invention.

[0030] Figure numerals: 1. conveying unit; 2. sample delivery unit; 3. feeding unit; 4. shaking device; 5. box; 6. culture space; 7. track module; 8. shooting unit; 9. receiving station; 10. sample delivery station; 11. feeding station; 12. feeding device; 13. barrel; 14. discharging mechanism; 15. driving cylinder; 16. pushing block; 17. storage tank; 18. transfer mechanism; 19. transfer port; 20. first cylinder slider; 21. telescopic cylinder; 22. transfer gun; 23. transfer chamber; 24. control Cylinder; 25. Piston; 26. Translation track; 27. Longitudinal moving device; 28. Track unit; 29. ​​Guide section; 30. Second cylinder slider; 31. Support plate; 32. Separation module; 33. Third cylinder slider; 34. Toggle rod; 35. Drive motor; 36. Conveyor belt; 37. Driven wheel; 38. Driving wheel; 39. Support frame; 40. Limit block; 41. Operation panel; 42. Cleaning unit; 43. Cleaning cylinder; 44. Cleaning chamber; 45. Nozzle; 46. Pretreatment space; 47. Fan. DETAILED DESCRIPTION

[0031] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments, wherein the same components are represented by the same reference numerals.

[0032] like Figure 1-7 As shown, a fully automatic microbial culture detection system includes a pretreatment module, a culture module and an automatic counting and calculation module.

[0033] Preferably, the pretreatment module comprises a conveying unit 1, a sample delivery unit 2 and a feeding unit 3, wherein the sample delivery unit 2 and the feeding unit 3 are used to respectively deliver the sample and the culture medium into the culture dish, and a shaking device 4 is provided at the end of the conveying unit 1.

[0034] Preferably, the culture module includes a box 5, a culture space 6 is formed in the box 5, a track module 7 is provided in the box 5, a conveying unit 1 is used to convey the culture dish to the track module 7, and a shooting unit 8 is provided at the end of the box 5 corresponding to the track module 7.

[0035] Preferably, the automatic counting and calculation module is used to automatically count and calculate the photos taken by the shooting unit 8.

[0036] When in use, the culture dish is transported by the conveying unit 1, and passes through the sample delivery unit 2 and the feeding unit 3 respectively. The sample delivery unit 2 and the feeding unit 3 respectively transport the sample and the culture medium into the culture dish according to the settings, and shake it through the shaking device 4 at the end of the conveying unit 1 to ensure that the sample and the culture medium are fully mixed. The culture dish is further transported to the track unit 28, that is, the culture space 6, by the conveying unit 1, and is moved by the track module 7 to drive multiple culture dishes to enter the culture space 6 in turn. After the culture is completed, the track module 7 continues to operate, driving the culture dish to be transferred to the end of the track module 7. At this time, the shooting unit 8 shoots the culture dish to form an image. After shooting, it is automatically counted by the optical system, and the result is automatically calculated according to the calculation formula pre-entered in the software. The completed culture dish is transported to the outside world driven by the track module 7 to complete the culture detection.

[0037] Preferably, the conveying unit 1 forms a receiving station 9, a sample delivery station 10 and a feeding station 11 along the conveying direction, respectively. The pretreatment module also includes a feeding device 12 arranged corresponding to the receiving station 9. The feeding device 12 is used to convey the culture dish to the conveying unit 1. The feeding device 12 includes a barrel 13, and the barrel 13 is vertically arranged. A discharging mechanism 14 is provided at the lower end of the barrel 13. The discharging mechanism 14 includes a driving cylinder 15 and a pushing block 16. The output end of the driving cylinder 15 is connected to the pushing block 16. The driving cylinder 15 extends out and pushes the culture dish to the conveying unit 1 through the pushing block 16. It is further preferred that the pushing block 16 is arc-shaped to adapt to the surface curve of the culture dish, thereby improving the position stability of the culture dish during transfer.

[0038] Specifically, a temporary storage space is formed at the lower end of the barrel 13, and the culture dishes are stacked in the barrel 13. The culture dish at the bottom naturally falls into the temporary storage space and is moved to the conveying unit 1 under the push of the driving cylinder 15. After the push is completed, the driving cylinder 15 retracts, and the culture dish above continues to fall into the temporary storage space to achieve automatic conveying.

[0039] Preferably, the sample delivery unit 2 and the feeding unit 3 both include a storage tank body 17 and a transfer mechanism 18. Furthermore, the feeding unit 3 is provided with an insulation device corresponding to the storage tank body 17, and the insulation device is composed of a heating rod and a temperature sensor, which is used to maintain the culture medium in the storage tank body 17 at a constant temperature of 45 degrees. A transfer port 19 is formed at the upper end of the storage tank body 17. The transfer mechanism 18 includes a first cylinder slider 20, a telescopic cylinder 21 and a transfer gun 22. The telescopic cylinder 21 is connected to the first cylinder slider 20, and the transfer gun 22 is connected to the output end of the telescopic cylinder 21. A transfer cavity 23 is formed in the transfer gun 22 and a liquid outlet is formed at the lower end. A control cylinder 24 and a piston 25 are provided in the transfer cavity 23. The control cylinder 24 is connected to the piston 25 and drives the piston 25 to move in the transfer cavity 23 to control the liquid outlet to absorb / discharge liquid.

[0040] When in use, the transfer gun 22 is moved to the storage tank body 17 by the first cylinder slider 20, and the telescopic cylinder 21 is further extended to make the transfer gun 22 move downward, and the liquid outlet is immersed in the liquid in the storage tank body 17, and the piston 25 is further driven to move by the control cylinder 24 to absorb the liquid in the storage tank body 17. After the absorption is completed, the telescopic cylinder 21 retracts and is transferred to the conveying unit 1 through the first cylinder slider 20, and the liquid discharge action is further completed by the control cylinder 24. The automatic sample delivery / feeding of the culture dish is realized by the setting of the first cylinder slider 20, the telescopic cylinder 21 and the transfer gun 22.

[0041] Preferably, the pretreatment module also includes a cleaning unit 42, which is used to clean the transfer gun 22. The cleaning unit 42 includes a cleaning cylinder 43, the upper end of which is open to form a cleaning chamber 44. A drain valve is provided at the bottom of the cleaning cylinder 43, and the drain valve is connected to the outside through a pipeline. A plurality of nozzles 45 are arranged circumferentially in the cleaning chamber 44.

[0042] During use, after the transfer gun 22 completes sample delivery for a single batch of culture dishes, the transfer gun is moved to the cleaning chamber 44 position by the first cylinder slider 20 and extended by the telescopic cylinder 21, so that the transfer gun 22 enters the cleaning chamber 44, and the cleaning liquid is further sprayed through the nozzle 45 to clean the transfer gun 22. After cleaning is completed, the waste water is discharged to the outside through the drain valve.

[0043] Preferably, the shaking device 4 includes a vibration generator, and the vibration generator is connected to the conveying unit 1. Specifically, the conveying unit 1 is any belt-type two-dimensional unidirectional conveying method in the prior art.

[0044] The provision of the vibration generator can make the overall structure more streamlined.

[0045] Preferably, the track module 7 includes a translation track 26 and a longitudinal moving device 27, and the translation track 26 and the longitudinal moving device 27 are used to drive the culture dish to move horizontally and vertically respectively.

[0046] The arrangement of the translation track 26 and the longitudinal moving device 27 can realize three-dimensional position adjustment of the culture dish, thereby adapting to the culture storage requirements of a large number of culture dishes.

[0047] Preferably, the translation track 26 includes a plurality of track units 28 arranged parallel to each other, and adjacent track units 28 have opposite driving directions. The translation track 26 also includes an arc-shaped guide section 29, and both ends of the guide section 29 are respectively connected to one end of two track units 28 connected to each other.

[0048] When in use, the culture dish is moved by the track unit 28, and the culture dish is transferred to the guide section 29 on the track unit 28. The subsequent culture dishes are continuously transferred to drive the original culture dish through the guide section 29 and enter another section of the track unit 28 to achieve the continued transfer of the culture dish.

[0049] The S-shaped track movement of the translation track 26 is achieved by the mutual cooperation of the track unit 28 and the guide section 29, thereby improving the space utilization of the culture space 6.

[0050] Preferably, the longitudinal moving device 27 includes a second cylinder slider 30 and a support plate 31. The second cylinder slider 30 is arranged corresponding to the moving passage. The support plate 31 and the second cylinder slider 30 are fixedly arranged. A separation module 32 is provided on the support plate 31. The separation module 32 is used to drive the culture dish to separate from the support plate 31 and move toward the translation track 26. The separation module 32 includes a third cylinder slider 33 and a toggle rod 34. The third cylinder slider 33 is arranged on the support plate 31, and the toggle rod 34 is connected to the third cylinder slider 33.

[0051] When in use, after the culture dish is transferred to the support plate 31, the second cylinder slider 30 drives the support plate 31 to move longitudinally, thereby transferring the culture dish to the predetermined culture space 6, and further the third cylinder slider 33 drives the toggle rod 34 to move, thereby contacting the culture dish and driving the culture dish to leave the support plate 31 and transfer it to the conveyor belt 36 for subsequent transmission.

[0052] The arrangement of the second cylinder slider 30 and the third cylinder slider 33 makes the overall volume of the longitudinal moving device 27 smaller, thereby reducing the space requirement and making the overall structure more compact.

[0053] Preferably, a temperature control unit and a humidity control unit are provided in the culture space 6. The temperature control unit and the humidity control unit are used to control the temperature and humidity in the culture space 6 respectively. The temperature control unit and the humidity unit are prior art and will not be described in detail here.

[0054] Furthermore, the box 5 also includes a pretreatment space 46, the pretreatment module is located in the pretreatment space 46, and the culture space 6 and the pretreatment space 46 are both provided with a sterilization component, which includes an ultraviolet light. Before use, the culture space 6 and the pretreatment space 46 are sterilized by turning on the ultraviolet light. Moreover, after a single sample is completed, the sterilization is completed for 30 minutes by the sterilization component to conduct the test of the next sample.

[0055] Furthermore, the box body 5 is further provided with a fan 47 corresponding to the pre-treatment space 46 , and the fan 47 is used to blow air outward to discharge the gas in the pre-treatment space 46 , thereby further reducing the pollution in the pre-treatment space 46 .

[0056] Preferably, the track unit 28 includes a driving motor 35, a conveyor belt 36 and a driven wheel 37. A driving wheel 38 is provided at the output end of the driving motor 35. The conveyor belt 36 is respectively wound around the driving wheel 38 and the driven wheel 37. A support frame 39 is provided corresponding to the conveyor belt 36 in the culture space 6. The support frame 39 is used to keep the conveyor belt 36 supported. Limit blocks 40 are arranged on the conveyor belt 36, and a accommodating space for accommodating culture dishes is formed between adjacent limit blocks 40.

[0057] The culture dish can be divided by setting the limit block 40, so that it is convenient for the shooting unit 8 to shoot a single culture dish, thereby avoiding shooting interference caused by multiple culture dishes being attached too close.

[0058] Preferably, an operation panel 41 is provided on one side of the box body 5, through which the longitudinal moving device 27 and the translation track 26 can be controlled, and the temperature control unit and the humidity control unit can also be controlled, thereby meeting the experimental needs.

[0059] At the same time, the culture time can be preset through the operation panel 41, and after the preset time is reached, the culture dishes are automatically transported by the translation track 26 and the longitudinal moving device 27 to achieve automatic photography of multiple groups of culture dishes.

[0060] Furthermore, the automatic counting and calculation module includes an automatic counting module and a calculation module set in the operation panel 41. When in use, the shooting unit 8 transmits the taken photos to the operation panel 41, and realizes the automatic counting function through the optical system. The result is further calculated by the calculation module and compared with the pre-built-in standard requirement value. The detection results that exceed the limit are bolded and displayed in a striking red color.

[0061] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A fully automatic microbial culture detection system, characterized in that: It includes a pretreatment module, a cultivation module and an automatic counting and calculation module; The pretreatment module comprises a conveying unit (1), a sample delivery unit (2) and a feeding unit (3), wherein the sample delivery unit (2) and the feeding unit (3) are used to respectively deliver the sample and the culture medium into the culture dish, and a shaking device (4) is provided at the end of the conveying unit (1); The culture module comprises a box (5), a culture space (6) is formed in the box (5), a track module (7) is arranged in the box (5), the conveying unit (1) is used to convey the culture dish to the track module (7), and a shooting unit (8) is arranged at the end of the box (5) corresponding to the track module (7); The automatic counting and calculation module is used to automatically count and calculate the photos taken by the shooting unit (8).

2. A fully automatic microbial culture detection system according to claim 1, characterized in that: The conveying unit (1) is respectively formed with a receiving station (9), a sample delivery station (10) and a feeding station (11) along the conveying direction. The pretreatment module also includes a feeding device (12) arranged corresponding to the receiving station (9). The feeding device (12) is used to transport the culture dish to the conveying unit (1). The feeding device (12) includes a barrel (13). The barrel (13) is arranged vertically. A discharge mechanism (14) is provided at the lower end of the barrel (13). The discharge mechanism (14) includes a driving cylinder (15) and a push block (16). The output end of the driving cylinder (15) is connected to the push block (16). The driving cylinder (15) extends and pushes the culture dish to the conveying unit (1) through the push block (16).

3. A fully automatic microbial culture detection system according to claim 1, characterized in that: The sample delivery unit (2) and the feeding unit (3) both include a storage tank body (17) and a transfer mechanism (18). The upper end of the storage tank body (17) is provided with a transfer port (19). The transfer mechanism (18) includes a first cylinder slider (20), a telescopic cylinder (21) and a transfer gun (22). The telescopic cylinder (21) is connected to the first cylinder slider (20). The transfer gun (22) is connected to the output end of the telescopic cylinder (21). A transfer chamber (23) is formed in the transfer gun (22) and a liquid outlet is formed at the lower end. A control cylinder (24) and a piston (25) are provided in the transfer chamber (23). The control cylinder (24) is connected to the piston (25) and drives the piston (25) to move in the transfer chamber (23) to control the liquid outlet to absorb / discharge liquid.

4. A fully automatic microbial culture detection system according to claim 1, characterized in that: The shaking device (4) comprises a vibration generator, and the vibration generator is connected to the conveying unit (1).

5. A fully automatic microbial culture detection system according to claim 1, characterized in that: The track module (7) comprises a translation track (26) and a longitudinal moving device (27), and the translation track (26) and the longitudinal moving device (27) are used to drive the culture dish to move horizontally and vertically respectively.

6. A fully automatic microbial culture detection system according to claim 5, characterized in that: The translation track (26) comprises a plurality of track units (28) arranged in parallel with each other, and the driving directions of adjacent track units (28) are opposite. The translation track (26) also comprises an arc-shaped guide section (29), and the two ends of the guide section (29) are respectively connected to one end of two track units (28) connected to each other.

7. A fully automatic microbial culture detection system according to claim 5, characterized in that: The longitudinal moving device (27) comprises a second cylinder slider (30) and a support plate (31); the second cylinder slider (30) is arranged corresponding to the moving path; the support plate (31) and the second cylinder slider (30) are fixedly arranged; a separation module (32) is arranged on the support plate (31); the separation module (32) is used to drive the culture dish to separate from the support plate (31) and move in the direction of the translation track (26); the separation module (32) comprises a third cylinder slider (33) and a toggle rod (34); the third cylinder slider (33) is arranged on the support plate (31); and the toggle rod (34) is connected to the third cylinder slider (33).

8. The fully automatic microbial culture detection system according to claim 1, characterized in that: A temperature control unit and a humidity control unit are provided in the culture space (6).

9. A fully automatic microbial culture detection system according to claim 6, characterized in that: The track unit (28) comprises a driving motor (35), a transmission belt (36) and a driven wheel (37); a driving wheel (38) is provided at the output end of the driving motor (35); the transmission belt (36) is wound around the driving wheel (38) and the driven wheel (37) respectively; a support frame (39) is provided in the culture space (6) corresponding to the transmission belt (36); the support frame (39) is used to keep the transmission belt (36) supported; a limiting block (40) is arranged on the transmission belt (36); and a containing space for containing a culture dish is formed between adjacent limiting blocks (40).

10. The fully automatic microbial culture detection system according to claim 1, characterized in that: An operation panel (41) is provided on one side of the box body (5).

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