Vegetable seedling and seedling strengthening microbial composition, matrix and preparation method

Abstract

The invention provides a vegetable seedling and seedling strengthening microbial composition, a substrate and a preparation method, and belongs to the technical field of microbial agents. The invention provides a microbial composition for vegetable seedling culture, the microbial composition comprises a bacterial liquid of various bacteria, the types of the bacteria comprise bacillus subtilis, bacillus amyloliquefaciens, paenibacillus polymyxa and azospirillum brazilianum, and the bacteria have a synergistic effect, so that the seed germination time can be remarkably shortened, and the root length and the lateral root number can be increased. According to the invention, the microbial composition is used as a basis, the microbial composition, mushroom wastes, straws, fresh livestock and poultry manure and the like are subjected to closed fermentation to prepare the matrix for vegetable seedling culture, seeds can be directly planted in the matrix for vegetable seedling culture, the seed germination time can be further shortened, and meanwhile, statistics is carried out within the same time after germination, and the result shows that the seed germination time is greatly shortened. Seedlings cultivated by using the matrix for vegetable seedling cultivation are more robust, and particularly, stem bases are thicker, leaves have larger surface areas, and the color is darker green.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial agents, and in particular relates to a vegetable seedling-growing and seedling-strengthening microbial composition and a matrix and a preparation method thereof. Background Art

[0002] With the adjustment of agricultural structure, the production of pollution-free high-quality vegetables has become an important part of the structural adjustment. In vegetable cultivation, the key to achieving high yield and high quality is to cultivate strong seedlings, and scientific fertilization and drug use are the most effective means.

[0003] At present, there are many kinds of fertilizers for vegetable production on the market, but the effects are single and it is difficult to meet the multiple requirements in seedling production. It requires the use of multiple fertilizers, which is time-consuming, labor-intensive and costly. Some fertilizers have rejection reactions, which is not conducive to the effectiveness of fertilizers and even has an adverse effect on vegetable growth. In addition, the effectiveness of existing fertilizers is relatively weak and needs to be further improved.

[0004] Commercial seedling cultivation generally uses peat, rock wool, vermiculite, perlite, bagasse, mushroom residue, gravel and ceramsite. At present, rock wool and peat are the most widely used in the world and are recognized as the ideal cultivation substrate in the world. However, with the increasing use of rock wool year by year, its negative effects on the social and ecological environment are becoming more and more obvious. On the one hand, since rock wool is non-degradable, its large-scale use brings secondary pollution to the environment; on the other hand, peat is a non-renewable resource, and excessive mining is in danger of depletion; therefore, seeking and exploring new seedling substrates that are easy to obtain, cheap, and can replace polluting materials such as rock wool has become one of the research hotspots of today's scientific researchers. Summary of the invention

[0005] The invention provides a vegetable seedling-growing and seedling-strengthening microbial composition and a matrix and a preparation method thereof, which can quickly induce vegetable seeds to germinate, have a high seedling rate, and produce strong seedlings.

[0006] The present invention provides a microbial composition for vegetable seedling cultivation, comprising the following bacterial liquids in parts by weight: 2 to 6 parts of a bacterial liquid of Bacillus subtilis, 1 to 3 parts of a bacterial liquid of Bacillus amyloliquefaciens, 4 to 8 parts of a bacterial liquid of Paenibacillus polymyxa, and 0.1 to 0.4 parts of a bacterial liquid of Azospirillum brasiliensis;

[0007] The number of cells in each bacterial solution should not be less than 10 7 cells / mL.

[0008] In a preferred embodiment of the present invention, the culture medium used in the preparation of each bacterial liquid includes the following components in concentrations: sucrose 1.0-1.5 g / L, peptone 1.5-1.9 g / L, yeast extract 2.7-3.6 g / L, K2HPO3·3H2O 0.3-0.6 g / L, NaCl 0.2-0.5 g / L and MgSO4·7H2O 0.1-0.2 g / L.

[0009] The present invention also provides a method for preparing the above-mentioned microbial composition for vegetable seedling cultivation, comprising the following steps: activating Bacillus subtilis, Bacillus amyloliquefaciens, Paenibacillus polymyxa and Azospirillum brasiliensis, inoculating them into a culture medium, and fermenting them at 28-35°C until OD 600 Not less than 0.6, the bacterial liquid is the microbial composition for vegetable seedling cultivation;

[0010] The culture medium comprises components with the following concentrations: sucrose 1.0-1.5 g / L, peptone 1.5-1.9 g / L, yeast extract 2.7-3.6 g / L, K2HPO3·3H2O 0.3-0.6 g / L, NaCl 0.2-0.5 g / L and MgSO4·7H2O 0.1-0.2 g / L.

[0011] In a preferred embodiment of the present invention, the fermentation is accompanied by shaking, and the frequency of the shaking is 180-220 rpm.

[0012] The present invention also provides a substrate for vegetable seedling cultivation, and the raw materials for preparation include the above-mentioned microbial composition for vegetable seedling cultivation or the microbial composition for vegetable seedling cultivation prepared by the above-mentioned preparation method, mushroom waste, straw and livestock and poultry excrement.

[0013] In a preferred embodiment of the present invention, the mass ratio of the microbial composition for vegetable seedling cultivation, mushroom waste, straw and fresh livestock and poultry manure is 5:(12-19):(7-15):6.

[0014] The present invention also provides a method for preparing the above-mentioned vegetable seedling substrate, comprising mixing the microbial composition for vegetable seedling cultivation, mushroom waste, straw and fresh livestock and poultry manure, fermenting them in a closed manner for 7 to 10 days, and drying to obtain the vegetable seedling substrate.

[0015] In a preferred embodiment of the present invention, the drying further comprises pulverizing.

[0016] The present invention also provides the use of the microbial composition for vegetable seedling cultivation or the microbial composition for vegetable seedling cultivation prepared by the preparation method or the matrix for vegetable seedling cultivation in vegetable seedling cultivation and seedling strengthening.

[0017] The present invention also provides a method for rapidly raising vegetable seedlings and obtaining strong seedlings, comprising mixing the above-mentioned microbial composition for raising vegetable seedlings or the microbial composition for raising vegetable seedlings prepared by the above-mentioned preparation method with vegetable seeds and then sowing the mixture in a substrate;

[0018] Or the vegetable seeds are sown in the above-mentioned vegetable seedling-raising matrix or the vegetable seedling-raising matrix prepared by the above-mentioned preparation method.

[0019] Beneficial effects: The present invention provides a microbial composition for vegetable seedling cultivation, comprising bacterial liquid of multiple bacteria, wherein the bacterial types include: Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus polymyxa and Azospirillum brasiliensis. The synergistic effect of the bacteria can significantly shorten the seed germination time and increase the root length and the number of lateral roots.

[0020] The present invention is also based on the microbial composition, and a vegetable seedling substrate is prepared through closed fermentation with mushroom waste, straw and fresh livestock and poultry manure. Seeds can be directly planted in the vegetable seedling substrate, which can further shorten the seed germination time. At the same time, statistics are performed within the same time after germination, and it is found that the seedlings grown using the vegetable seedling substrate of the present invention are stronger, which is specifically manifested in that the base of the stem is thicker, the leaves have a larger surface area, and the color is darker green. DETAILED DESCRIPTION

[0021] The present invention provides a microbial composition for vegetable seedling cultivation, comprising the following bacterial liquids in parts by weight: 2 to 6 parts of a bacterial liquid of Bacillus subtilis, 1 to 3 parts of a bacterial liquid of Bacillus amyloliquefaciens, 4 to 8 parts of a bacterial liquid of Paenibacillus polymyxa, and 0.1 to 0.4 parts of a bacterial liquid of Azospirillum brasiliensis;

[0022] The number of cells in each bacterial solution should not be less than 10 7 cells / mL.

[0023] The present invention does not specifically limit the source of each bacterium, and common commercially available bacterial powder in the art can be used, and the bacterial powder purchased on the market can be activated according to the contents of the instructions; then each activated bacterial liquid can be inoculated into a culture medium for separate culture, or each activated bacterial liquid can be mixed and inoculated into a culture medium for mixed culture, and any two or three activated bacterial liquids can be inoculated into a culture medium for culture, and then mixed with the culture liquid of other bacteria.

[0024] The Bacillus subtilis of the present invention can promote the rapid growth of vegetable seedlings, and the Bacillus amyloliquefaciens can produce plant growth regulators, such as auxin and gibberellins, thereby promoting the growth and development of plants. At the same time, the Bacillus amyloliquefaciens can synergize with the Bacillus subtilis to jointly stimulate early germination of seeds. It is verified by the examples that the same batch of seeds, under the combined action of the Bacillus amyloliquefaciens and the Bacillus subtilis, have a shorter germination time than the single treatment of the Bacillus amyloliquefaciens and the Bacillus subtilis and the water soaking treatment, compared with the water soaking, the early time is reduced once.

[0025] The polymyxa bacillus of the present invention has a broad-spectrum bactericidal effect and has preventive effects on a variety of vegetable diseases, such as taro soft rot, Chinese cabbage soft rot, pepper root rot, tomato damping-off, tomato damping-off and pepper blight, and can also promote the rapid growth of vegetable seedlings; the Brazilian nitrogen-fixing spirulina can cope with a variety of adverse environments, such as high temperature and adverse pH, and has a high nitrogenase activity, as well as considerable phosphorus solubilization characteristics and the effect of promoting crop growth. The combination of the polymyxa bacillus and the Brazilian nitrogen-fixing spirulina of the present invention can reduce the amount of fertilizers, pesticides and fungicides used in the process of vegetable seedling cultivation, and ensure the rapid growth of vegetable seedlings in a variety of environments.

[0026] The combined use of the four bacteria of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus polymyxa and Azospirillum brasiliensis described in the present invention can also achieve a more efficient promotion effect. For example, vegetable seeds can also germinate quickly on saline-alkali soil and grow into strong seedlings with a high seedling rate. In multiple indoor simulation experiments of the embodiments, without the additional use of fertilizers, pesticides and fungicides, various types of vegetable seedlings are almost free from diseases, and they survive quickly after thinning and transplanting. Even when the seedlings are transplanted to saline-alkali land, the survival rate of the vegetable seedlings is higher than 70%, which also produces a synergistic effect.

[0027] In a preferred embodiment of the present invention, the same culture medium can be used to prepare the bacterial solution of each activated bacterium, and the culture medium contains sucrose, and the sucrose content can reach 1.0-1.5 g / L, such as 1.0 g / L, 1.1 g / L, 1.2 g / L, 1.3 g / L, 1.4 g / L or 1.5 g / L; the culture medium contains a peptone component, and the peptone content is 1.5-1.9 g / L, such as 1.5 g / L, 1.6 g / L, 1.7 g / L, 1.8 g / L or 1.9 g / L; the culture medium contains yeast extract, and the yeast extract content is 2.7-3.6 g / L, such as 2.7 g / L, 2.8 g / L, 2.9 g / L, 3.0 g / L, 4.1 g / L, 5.2 g / L, 6.8 g / L, 7.9 g / L, 8.1 g / L, 9.2 g / L, 10. 3.1g / L, 3.2g / L, 3.3g / L, 3.4g / L, 3.5g / L or 3.6g / L; the culture medium contains K2HPO3·3H2O, and the content of K2HPO3·3H2O is 0.3-0.6g / L, such as 0.3g / L, 0.4g / L, 0.5g / L or 0.6g / L; the culture medium contains NaCl, and the content of NaCl is 0.2-0.5g / L, such as 0.2g / L, 0.3g / L, 0.4g / L or 0.5g / L; the culture medium also contains MgSO4·7H2O, and the content of MgSO4·7H2O is 0.1-0.2g / L, such as 0.1g / L or 0.2g / L.

[0028] The present invention also provides a method for preparing the above-mentioned microbial composition for vegetable seedling cultivation, comprising the following steps: activating Bacillus subtilis, Bacillus amyloliquefaciens, Paenibacillus polymyxa and Azospirillum brasiliensis, inoculating them into a culture medium, and fermenting them at 28-35°C until OD 600 Not less than 0.6, the bacterial liquid is the microbial composition for vegetable seedling cultivation;

[0029] The culture medium comprises components with the following concentrations: sucrose 1.0-1.5 g / L, peptone 1.5-1.9 g / L, yeast extract 2.7-3.6 g / L, K2HPO3·3H2O 0.3-0.6 g / L, NaCl 0.2-0.5 g / L and MgSO4·7H2O 0.1-0.2 g / L.

[0030] In the present invention, the microbial composition for vegetable seedling cultivation, i.e., mixed bacterial solution, can be obtained by various methods, such as co-culturing after separate activation, sequential culturing after separate activation, separate culturing after separate activation and then mixing or other methods. In the embodiment, co-culturing is used as an example for explanation. In the embodiment of the present invention, four culturings are performed: culturing at 28-35°C accompanied by shaking, the shaking frequency is 180-220rpm, culturing alone for a certain period of time, until the bacterial solution OD 600 Not less than 0.6, the number of live bacteria of each bacteria is not less than 10 7cells / mL, and then mix according to the proportion.

[0031] The present invention also provides a substrate for vegetable seedling cultivation, and the raw materials for preparation include the above-mentioned microbial composition for vegetable seedling cultivation or the microbial composition for vegetable seedling cultivation prepared by the above-mentioned preparation method, mushroom waste, straw and livestock and poultry excrement.

[0032] In a preferred embodiment of the present invention, the mass ratio of the microbial composition for vegetable seedling cultivation, mushroom waste, straw and fresh livestock and poultry manure is 5:(12-19):(7-15):6, the ratio with a better effect is 5:(13-17):(9-12):6, and the ratio with the best effect is 5:15:10:6.

[0033] The mushroom waste of the present invention is the residues of edible mushrooms after they have been cultured and grown, such as the residues of straw mushrooms, oyster mushrooms, king oyster mushrooms and enoki mushrooms, which can all be used as the mushroom waste of the present invention.

[0034] The straw of the present invention can be fresh straw or dried and then moistened straw, as long as the moisture content of the straw is not less than 10%; the straw used can be wheat straw, corn straw, peanut straw or even soybean straw.

[0035] The fresh livestock and poultry manure of the present invention can be collected from cattle farms, pig farms, chicken farms, etc., and does not need to be sterilized. The above-mentioned microbial composition, mushroom waste, straw and fresh livestock and poultry manure are directly mixed in proportion and then directly subjected to sealed anaerobic fermentation. However, during the fermentation process, if the temperature is higher than 60°C, heat can be dissipated, such as by ventilation heat dissipation, and fermentation can be performed until there is no obvious odor and the temperature is maintained at 30±2°C.

[0036] The present invention also provides a method for preparing the above-mentioned vegetable seedling substrate, comprising mixing the microbial composition for vegetable seedling cultivation, mushroom waste, straw and fresh livestock and poultry manure, fermenting them in a closed manner for 7 to 10 days, and drying to obtain the vegetable seedling substrate.

[0037] In a preferred embodiment of the present invention, the drying step further comprises crushing into particles with a particle diameter of 0.1 to 0.5 cm.

[0038] The vegetable seedling raising substrate of the present invention is rich in easily-absorbed organic nutrients required for the growth and development of seedlings, and has a wide variety of nutrients, and can be used instead of base fertilizer and topdressing.

[0039] The present invention also provides the use of the microbial composition for vegetable seedling cultivation or the microbial composition for vegetable seedling cultivation prepared by the preparation method or the matrix for vegetable seedling cultivation in vegetable seedling cultivation and seedling strengthening.

[0040] Vegetable seedlings can be grown using the microbial composition for vegetable seedling cultivation or the matrix for vegetable seedling cultivation of the present invention, and can germinate quickly, grow rapidly, and be stronger than seedlings of the same age.

[0041] The present invention also provides a method for rapidly raising vegetable seedlings and obtaining strong seedlings, comprising mixing the above-mentioned microbial composition for raising vegetable seedlings or the microbial composition for raising vegetable seedlings prepared by the above-mentioned preparation method with vegetable seeds and then sowing the mixture in a substrate;

[0042] Or the vegetable seeds are sown in the above-mentioned vegetable seedling-raising matrix or the vegetable seedling-raising matrix prepared by the above-mentioned preparation method.

[0043] In one embodiment of the present invention, the vegetable seedling matrix can be directly used as a seedling matrix, and the vegetable seeds to be planted can be directly sown in the seedling matrix. No fertilizer is needed during the seedling period, and seedlings can be grown quickly, reducing the seedling time.

[0044] In another embodiment of the present invention, the vegetable seeds can be soaked in the microbial composition for vegetable seedling cultivation for 10 to 30 minutes, such as 10 minutes, 12 minutes, 15 minutes, 20 minutes, 21 minutes, 25 minutes or 30 minutes. After soaking, the vegetable seeds are directly sown in a seedling cultivation matrix, which can be a common seedling cultivation matrix, such as a mixed matrix of vermiculite, organic matter and soil, or a vegetable seedling cultivation matrix prepared by the present invention.

[0045] In order to further illustrate the present invention, a vegetable seedling-growing and seedling-strengthening microbial composition, a matrix and a preparation method provided by the present invention are described in detail below in conjunction with embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0046] The strains used in the embodiments of the present invention are all conventional commercially available products, among which Bacillus subtilis was purchased from Okobaike (item number ok305), Bacillus amyloliquefaciens (powder) and Bacillus polymyxa (powder) were purchased from Hebei Liangze Biotechnology Co., Ltd., and Azospirillum brasiliensis was purchased from Shanghai Xuanke Biotechnology Co., Ltd. (item number XK-SH-6596); before use, the above strains were activated according to the instructions.

[0047] Example 1

[0048] After activation, Bacillus subtilis, Bacillus amyloliquefaciens, Paenibacillus polymyxa and Azospirillum brasiliensis were inoculated into the culture medium and fermented at 28°C until OD 600 Not less than 0.6, at which point the number of cells in the bacterial solution is not less than 10 7 cells / mL; then the four bacterial solutions were mixed in a volume ratio of 5:2:6:0.3;

[0049] Medium components: sucrose 1.0 g / L, peptone 1.9 g / L, yeast extract 3.6 g / L, K2HPO3·3H2O 0.6 g / L, NaCl 0.5 g / L and MgSO4·7H2O 0.2 g / L.

[0050] The mixed bacterial liquid, mushroom waste, straw and fresh livestock and poultry excrement are mixed, fermented in a sealed manner for 7 to 10 days, and dried to obtain the vegetable seedling culture medium.

[0051] Example 2

[0052] After activation, Bacillus subtilis, Bacillus amyloliquefaciens, Paenibacillus polymyxa and Azospirillum brasiliensis were inoculated into the culture medium and fermented at 30°C until OD 600 Not less than 0.6, at which point the number of cells in the bacterial solution is not less than 10 7 cells / mL; then the four bacterial solutions were mixed in a volume ratio of 2:2:8:0.2;

[0053] Medium components: sucrose 1.3 g / L, peptone 1.5 g / L, yeast extract 3.0 g / L, K2HPO3·3H2O 0.5 g / L, NaCl 0.3 g / L and MgSO4·7H2O 0.2 g / L.

[0054] The mixed bacterial liquid, mushroom waste, straw and fresh livestock and poultry excrement are mixed, fermented in a sealed manner for 7 to 10 days, and dried to obtain the vegetable seedling culture medium.

[0055] Example 3

[0056] After activation, Bacillus subtilis, Bacillus amyloliquefaciens, Paenibacillus polymyxa and Azospirillum brasiliensis were inoculated into the culture medium and fermented at 32°C until OD 600 Not less than 0.6, at which point the number of cells in the bacterial solution is not less than 10 7 cells / mL; then the four bacterial solutions were mixed in a volume ratio of 2:3:6:0.1;

[0057] Medium components: sucrose 1.2 g / L, peptone 1.8 g / L, yeast extract 3.3 g / L, K2HPO3·3H2O 0.4 g / L, NaCl 0.4 g / L and MgSO4·7H2O 0.1 g / L.

[0058] The mixed bacterial liquid, mushroom waste, straw and fresh livestock and poultry excrement are mixed, fermented in a sealed manner for 7 to 10 days, and dried to obtain the vegetable seedling culture medium.

[0059] Example 4

[0060] After activation, Bacillus subtilis, Bacillus amyloliquefaciens, Paenibacillus polymyxa and Azospirillum brasiliensis were inoculated into the culture medium and fermented at 35°C until OD 600 Not less than 0.6, at which point the number of cells in the bacterial solution is not less than 10 7 cells / mL; then the four bacterial solutions were mixed in a volume ratio of 3:1:4:0.3;

[0061] Medium components: sucrose 1.5 g / L, peptone 1.6 g / L, yeast extract 2.8 g / L, K2HPO3·3H2O 0.5 g / L, NaCl 0.3 g / L and MgSO4·7H2O 0.2 g / L.

[0062] The mixed bacterial liquid, mushroom waste, straw and fresh livestock and poultry excrement are mixed, fermented in a sealed manner for 7 to 10 days, and dried to obtain the vegetable seedling culture medium.

[0063] Example 5

[0064] After activation, Bacillus subtilis, Bacillus amyloliquefaciens, Paenibacillus polymyxa and Azospirillum brasiliensis were inoculated into the culture medium and fermented at 33°C until OD 600 Not less than 0.6, at which point the number of cells in the bacterial solution is not less than 10 7 cells / mL; then the four bacterial solutions were mixed in a volume ratio of 4:2:4:0.3;

[0065] Medium components: sucrose 1.2 g / L, peptone 1.6 g / L, yeast extract 3.1 g / L, K2HPO3·3H2O 0.4 g / L, NaCl 0.4 g / L and MgSO4·7H2O 0.1 g / L.

[0066] The mixed bacterial liquid, mushroom waste, straw and fresh livestock and poultry excrement are mixed, fermented in a sealed manner for 7 to 10 days, and dried to obtain the vegetable seedling culture medium.

[0067] Example 6

[0068] After activation, Bacillus subtilis, Bacillus amyloliquefaciens, Paenibacillus polymyxa and Azospirillum brasiliensis were inoculated into the culture medium and fermented at 28°C until OD 600 Not less than 0.6, at which point the number of cells in the bacterial solution is not less than 10 7 cells / mL; then the four bacterial solutions were mixed in a volume ratio of 2:3:8:0.4;

[0069] Medium components: sucrose 1.0 g / L, peptone 1.7 g / L, yeast extract 3.4 g / L, K2HPO3·3H2O 0.4 g / L, NaCl 0.2 g / L and MgSO4·7H2O 0.2 g / L.

[0070] The mixed bacterial liquid, mushroom waste, straw and fresh livestock and poultry excrement are mixed, fermented in a sealed manner for 7 to 10 days, and dried to obtain the vegetable seedling culture medium.

[0071] Comparative tests were conducted using the six mixed bacterial solutions and vegetable seedling substrates prepared in Examples 1 to 6, and a control example was set. In the comparative test, seedlings were grown indoors in seedling cups. The vegetable seeds for seedlings were purchased from the market. The vegetable types were: radish, green onion, celery, cabbage, pepper, tomato, coriander and spinach. Each experiment was repeated three times, and 50 seeds were sown in each seedling cup.

[0072] The settings are as follows:

[0073] Control Example 1: Using loess as the substrate, directly sow the purchased vegetable seeds in the seedling cup and water thoroughly;

[0074] Control Example 2: Using loess as the substrate, apply 10g of organic fertilizer before sowing, then sow vegetable seeds and water thoroughly;

[0075] Control Example 3: Using loess as the substrate, 10 g of compound fertilizer (NPK = 15-15-15) was applied before sowing, and then vegetable seeds were sown and watered thoroughly;

[0076] Control Example 4: Using a mixed matrix of peat soil and vermiculite in a ratio of 7:3 as the matrix, 10g of organic fertilizer was applied before sowing, and then vegetable seeds were sown and watered thoroughly;

[0077] Control Example 5: Using a mixed matrix of peat soil and vermiculite in a ratio of 7:3 as the matrix, 10 g of compound fertilizer (NPK = 15-15-15) was applied before sowing, and then vegetable seeds were sown and watered thoroughly;

[0078] Control Example 6: Using a mixed matrix of peat soil, vermiculite and perlite in a ratio of 1:1:1 as the matrix, applying 10g of organic fertilizer before sowing, and then sowing vegetable seeds and irrigating thoroughly;

[0079] Control Example 7: Using a mixed matrix of peat soil, vermiculite and perlite in a ratio of 1:1:1 as the matrix, 10g of compound fertilizer (NPK=15-15-15) was applied before sowing, and then vegetable seeds were sown and watered thoroughly;

[0080] Experimental Example 1: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Example 1 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0081] Experimental Example 2: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Example 2 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0082] Experimental Example 3: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Example 3 for 15 minutes, then sown in the seedling cups and watered thoroughly;

[0083] Experimental Example 4: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Example 4 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0084] Experimental Example 5: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Example 5 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0085] Experimental Example 6: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Example 6 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0086] Experimental Example 7: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis in Example 1 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0087] Experimental Example 8: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis in Example 4 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0088] Experimental Example 9: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Paenibacillus polymyxa and Azospirillum brasiliensis in Example 1 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0089] Experimental Example 10: Using loess as the substrate, the purchased vegetable seeds were soaked in the mixed bacterial solution of Paenibacillus polymyxa and Azospirillum brasiliensis in Example 4 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0090] Experimental Example 11: Using a mixed matrix prepared with peat soil and vermiculite in a ratio of 7:3 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis in Example 1 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0091] Experimental Example 12: Using a mixed matrix prepared with peat soil and vermiculite in a ratio of 7:3 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis in Example 2 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0092] Experimental Example 13: Using a mixed matrix prepared with peat soil and vermiculite in a ratio of 7:3 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis in Example 3 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0093] Experimental Example 14: Using a mixed matrix prepared with peat soil and vermiculite in a ratio of 7:3 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis in Example 4 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0094] Experimental Example 15: Using a mixed matrix prepared with peat soil and vermiculite in a ratio of 7:3 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis in Example 5 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0095] Experimental Example 16: Using a mixed matrix prepared with peat soil and vermiculite in a ratio of 7:3 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis in Example 6 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0096] Experimental Example 17: Using a mixed matrix prepared with peat soil, vermiculite and perlite in a ratio of 1:1:1 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Paenibacillus polymyxa and Azospirillum brasiliensis in Example 1 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0097] Experimental Example 18: Using a mixed matrix prepared with peat soil, vermiculite and perlite in a ratio of 1:1:1 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Paenibacillus polymyxa and Azospirillum brasiliensis in Example 2 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0098] Experimental Example 19: Using a mixed matrix prepared with peat soil, vermiculite and perlite in a ratio of 1:1:1 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Paenibacillus polymyxa and Azospirillum brasiliensis in Example 3 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0099] Experimental Example 20: Using a mixed matrix prepared with peat soil, vermiculite and perlite in a ratio of 1:1:1 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Paenibacillus polymyxa and Azospirillum brasiliensis in Example 4 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0100] Experimental Example 21: Using a mixed matrix prepared with peat soil, vermiculite and perlite in a ratio of 1:1:1 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Paenibacillus polymyxa and Azospirillum brasiliensis in Example 5 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0101] Experimental Example 22: Using a mixed matrix prepared with peat soil, vermiculite and perlite in a ratio of 1:1:1 as the matrix, the purchased vegetable seeds were soaked in the mixed bacterial solution of Paenibacillus polymyxa and Azospirillum brasiliensis in Example 6 for 15 minutes, then sown in a seedling cup and watered thoroughly;

[0102] Experimental Example 23: Using the vegetable seedling substrate prepared in Example 1 as the substrate, the purchased vegetable seeds were directly sown in the seedling cups and watered thoroughly;

[0103] Experimental Example 24: Using the vegetable seedling substrate prepared in Example 2 as the substrate, the purchased vegetable seeds were directly sown in the seedling cups and watered thoroughly;

[0104] Experimental Example 25: Using the vegetable seedling substrate prepared in Example 3 as the substrate, the purchased vegetable seeds were directly sown in the seedling cups and watered thoroughly;

[0105] Experimental Example 26: Using the vegetable seedling substrate prepared in Example 4 as the substrate, the purchased vegetable seeds were directly sown in the seedling cups and watered thoroughly;

[0106] Experimental Example 27: Using the vegetable seedling substrate prepared in Example 5 as the substrate, the purchased vegetable seeds were directly sown in the seedling cups and watered thoroughly;

[0107] Experimental Example 28: Using the vegetable seedling substrate prepared in Example 6 as the substrate, the purchased vegetable seeds were directly sown in the seedling cups and watered thoroughly.

[0108] Statistics on germination time, commercial seedling maturity date and growth potential within a specific time period were conducted for the vegetables in each control and experimental example;

[0109] The statistical method of seedling growth index is as follows: 6 seedlings were randomly selected from each treatment for growth index determination during sampling. The roots and plants were rinsed with clean water, the plant height was measured with a steel ruler, the stem diameter was measured with a vernier caliper, the roots and the aboveground parts were cut, and the fresh weight of the roots and the aboveground parts were weighed with an electronic balance. The roots and the aboveground parts were dried and then weighed for dry weight. The seedling index was calculated according to the formula: (stem diameter / plant height+underground dry weight / aboveground dry weight)×whole plant dry weight.

[0110] After sowing the radish seeds, the control examples 1 to 7 did not germinate before the 7th day, and began to germinate on the 7th day; after being treated with the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis, they could germinate a little earlier, on the 5th day, and when using various vegetable seedling substrates as the substrate, they began to germinate on the 3rd day, more than half of them had germinated on the 5th day, and the germination rate reached the highest on the 8th day.

[0111] Table 1 Statistics of germination rate of radish after sowing

[0112]

[0113]

[0114]

[0115] After sowing the green onion seeds, the control examples 1 to 6 began to germinate a small amount on the 12th day, and the germination rate reached the highest level of 80% on the 15th day; while the experimental examples 1 to 6 began to germinate a small amount on the 9th day, the germination rate reached about 80% on the 12th day, and the germination rate reached 97.33% on the 15th day; in the embodiments 23 to 28, a small amount of germination began on the 7th day, the germination rate reached 52.67% on the 9th day, the germination rate reached 90% on the 10th day, and the germination rate reached 100% on the 11th day.

[0116] After sowing celery seeds, control examples 1-6 began to germinate in small amounts on the 17th day, and the germination rate was the highest at 21 days, reaching 87%, and reached 5-6 leaves at about 52 days after sowing, but the seedlings were thin and weak, and the strong seedling index was only 0.73, and they needed to be cultivated again before they could be transplanted as commercial seedlings; experimental examples 1-6 began to germinate in small amounts on the 15th day, and the germination rate reached about 50% at 17 days, and the germination rate reached 86% at 18 days, and reached 5-6 leaves at 49 days after sowing, but the seedlings were thin, the leaves were fat and green, and the strong seedling index was 1.12, which could be used as commercial seedlings for transplanting; in experimental examples 23-28, a small amount of germination began to germinate on the 12th day, the germination rate exceeded 80% at 15 days, the germination rate was 100% at 20 days, and reached 5-6 leaves at 43 days, the seedlings were strong, the leaves were fat and green, and the strong seedling index was 1.38, which could be used as commercial seedlings for transplanting.

[0117] After the cabbage seeds were sown, the control examples 1-6 began to germinate on the 4th day, and the germination rate was the highest on the 7th day, reaching 90%, and reached 3-4 leaves 18 days after sowing, but the seedlings were slender, and the seedling index was 0.79, and they needed to be cultivated again before they could be transplanted as commercial seedlings; in the experimental examples 1-6, a large number of sprouts sprouted on the 3rd day, the germination rate was more than half on the 4th day, and they were basically all out on the 5th day, and reached 3-4 leaves on the 16th day. At this time, the leaves were relatively green, and the seedling index was 1.07; in the experimental examples 23-28, more than half of the sprouts sprouted on the 2nd day after sowing, the germination rate reached 99% on the 3rd day, and reached 3-4 leaves on the 12th day. At this time, the seedling stems were relatively thick, the growth was good, and the seedling index was 1.46, which could be transplanted as commercial seedlings.

[0118] After the pepper seeds were sown, the control examples 1 to 6 began to germinate on the 6th day, germinated in large quantities on the 8th day, and were basically all grown on the 10th day, but some seeds still germinated later. 44 days after sowing, the 8th to 9th leaves were basically grown, and the strong seedling index was 1.05, which could be used as commercial seedlings for transplanting; in embodiments 1 to 6, the seeds began to germinate on the 4th day, the germination rate exceeded 50% on the 6th day, the germination rate reached up to 98% on the 8th day, and 8 to 9 leaves were grown on the 40th day, the strong seedling index was 1.20, and they could be used as commercial seedlings for transplanting; in embodiments 23 to 28, the germination rate was more than 20% on the 3rd day of sowing, the germination rate exceeded 70% on the 4th day, and they were basically all grown on the 5th day. When counted on the 6th day, the germination rate reached 100%, and 8 to 9 leaves were grown on the 33rd day, the strong seedling index was 1.35, and they could be used as commercial seedlings for transplanting. The pepper seedlings obtained by different seedling raising methods were transplanted into ordinary fields using the same conventional means. The transplant survival rate within 5 days of transplanting was statistically calculated. The average transplant survival rate of control examples 1 to 6 could reach 87%, and the average transplant survival rate of embodiments 23 to 28 could reach 95%.

[0119] After sowing tomato seeds, control examples 1 to 6 began to have seedlings sprout on the 7th day, the germination rate was more than half on the 8th day, and basically all the seedlings emerged on the 10th day. 40 days after sowing, 4 to 5 leaves grew, but the leaves of the seedlings were yellow and the leaf tips were dry; in embodiments 1 to 6, the germination rate exceeded 20% on the 5th day, the germination rate reached 80% on the 7th day, and 4 to 5 leaves grew 37 days after sowing, but the seedlings were slender; in embodiments 23 to 28, the germination rate reached 82% on the 5th day, the germination rate exceeded 93% on the 6th day, and 4 to 5 leaves grew on the 32nd day. The leaves of the seedlings were bright green, the growth was good, and the seedling index was 1.52, which could be directly used as commercial seedlings.

[0120] Coriander seeds are similar to spinach seeds. In control examples 1 to 6, the germination rate is about 20% on the 9th day, of which the germination rate of coriander reaches a maximum of 87% on the 12th day, and the germination rate of spinach seeds reaches a maximum of 92% on the 14th day; in embodiments 23 to 28, the germination rate of coriander and spinach exceeds 50% on the 7th day, the germination rate of coriander reaches 100% on the 10th day, and the germination rate of spinach reaches 100% on the 9th day. The coriander seedlings and spinach seedlings are transplanted into flower pots added with simulated saline-alkali loess (containing 7g / kg NaCl), and the transplant survival rate of the same transplanting method is statistically calculated. On the 5th day, the survival rate of coriander seedlings and spinach seedlings in control examples 1 to 6 is low, less than 40%, and the growth is extremely weak; the survival rate of coriander seedlings and spinach seedlings in embodiments 23 to 28 is about 80%, and the growth is normal.

[0121] In order to verify the synergistic effect of the four strains of the present invention and the synergistic effect of the vegetable seedling substrate prepared based on the strains, cucumber was used as the experimental object, and the bacterial solution, mixed bacterial solution and vegetable seedling substrate prepared in Example 1 and Example 3 were used for data statistics. When conducting the experiment, a ceramic flower pot with a diameter of 23 cm was used as a container. When soaking in bacterial solution, loess was used as the substrate, and soaked for 15 minutes respectively, and then 20 seeds were sown in each flower pot; when treating the vegetable seedling substrate, the vegetable seedling substrate was directly used as the substrate, without soaking the seeds, and directly sowed, and 20 seeds were sown in each pot; each treatment was repeated three times, and the average was used for statistics. The standard for commercial cucumber seedlings is 2 to 3 leaves.

[0122] In Table 2, item A is for soaking the seeds in the bacterial solution of Bacillus amyloliquefaciens, B is for soaking the seeds in the bacterial solution of Bacillus subtilis, C is for soaking the seeds in the bacterial solution of Bacillus polymyxa, D is for soaking the seeds in the bacterial solution of Azospirillum brasiliensis, A+B is for soaking the seeds in the mixed bacterial solution of Bacillus amyloliquefaciens and Bacillus subtilis, C+D is for soaking the seeds in the mixed bacterial solution of Bacillus polymyxa and Azospirillum brasiliensis, A+B+C+D is for soaking the seeds in the mixed bacterial solution of Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus polymyxa and Azospirillum brasiliensis, and E is a matrix for vegetable seedling cultivation.

[0123] As shown in Table 2, compared with treatments C and D, treatments A and B can germinate earlier, and have a higher germination rate at 5 days, and the time to commercial seedlings is similar; but after treatment A+B, compared with treatments A and B, the initial germination time is advanced again, and the germination rate at 5 days is doubled, that is, Bacillus amyloliquefaciens and Bacillus subtilis have the effect of synergistically advancing the initial germination time and improving the germination rate; compared with treatments C and D, the seedling index of treatment C+D is significantly improved, and it has a significant synergistic effect on the seedling rate of commercial seedlings; but there is no significant difference between treatment A+B+C+D and treatment A+B and treatment C+D; compared with treatment A+B+C+D, treatment A+B and treatment C+D, treatment E has significantly improved initial germination time, early germination rate, time to commercial seedlings and seedling index, that is, after being prepared into a matrix for vegetable seedling cultivation, it also produces a significant synergistic effect compared with the bacterial solution soaking treatment.

[0124] Table 2 Statistics of cucumber seed germination and seedlings

[0125]

[0126]

[0127] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A microbial composition for vegetable seedling cultivation, characterized in that: The method comprises the following bacterial liquids in parts by weight: 2 to 6 parts of a bacterial liquid of Bacillus subtilis, 1 to 3 parts of a bacterial liquid of Bacillus amyloliquefaciens, 4 to 8 parts of a bacterial liquid of Paenibacillus polymyxa, and 0.1 to 0.4 parts of a bacterial liquid of Azospirillum brasiliensis; The number of cells in each bacterial solution should not be less than 10 7 cells / mL.

2. The microbial composition for vegetable seedling cultivation according to claim 1, characterized in that: The culture medium used in the preparation of each bacterial liquid includes the following components in concentrations: sucrose 1.0-1.5 g / L, peptone 1.5-1.9 g / L, yeast extract 2.7-3.6 g / L, K2HPO3·3H2O 0.3-0.6 g / L, NaCl 0.2-0.5 g / L and MgSO4·7H2O 0.1-0.2 g / L.

3. The method for preparing the microbial composition for vegetable seedling cultivation according to claim 1 or 2, characterized in that: The following steps are involved: After activation, Bacillus subtilis, Bacillus amyloliquefaciens, Paenibacillus polymyxa and Azospirillum brasiliensis were inoculated into the culture medium and fermented at 28-35°C until OD 600 Not less than 0.6, the bacterial liquid is the microbial composition for vegetable seedling cultivation; The culture medium comprises components with the following concentrations: sucrose 1.0-1.5 g / L, peptone 1.5-1.9 g / L, yeast extract 2.7-3.6 g / L, K2HPO3·3H2O 0.3-0.6 g / L, NaCl 0.2-0.5 g / L and MgSO4·7H2O 0.1-0.2 g / L.

4. The preparation method according to claim 3, characterized in that: The fermentation is accompanied by shaking, and the frequency of the shaking is 180-220 rpm.

5. A substrate for raising vegetable seedlings, characterized in that: The preparation raw materials include the microbial composition for vegetable seedling cultivation according to claim 1 or 2 or the microbial composition for vegetable seedling cultivation prepared by the preparation method according to claim 3 or 4, mushroom waste, straw and livestock and poultry excrement.

6. The vegetable seedling raising substrate according to claim 5, characterized in that: The mass ratio of the microbial composition for vegetable seedling cultivation, mushroom waste, straw and fresh livestock and poultry manure is 5:(12-19):(7-15):

6.

7. The method for preparing a substrate for raising vegetable seedlings according to claim 5 or 6, characterized in that: The method comprises mixing the microbial composition for vegetable seedling cultivation, mushroom waste, straw and fresh livestock and poultry excrement, fermenting them in a sealed manner for 7 to 10 days, and drying to obtain the substrate for vegetable seedling cultivation.

8. The preparation method according to claim 7, characterized in that: The drying step further includes pulverizing.

9. Use of the microbial composition for vegetable seedling cultivation according to claim 1 or 2, or the microbial composition for vegetable seedling cultivation prepared by the preparation method according to claim 3 or 4, or the matrix for vegetable seedling cultivation according to claim 5 or 6 in vegetable seedling cultivation and seedling strengthening.

10. A method for rapidly raising vegetable seedlings and obtaining strong seedlings, characterized in that: The method comprises mixing the microbial composition for vegetable seedling cultivation according to claim 1 or 2 or the microbial composition for vegetable seedling cultivation prepared by the preparation method according to claim 3 or 4 with vegetable seeds and then sowing the mixture in a substrate; Or sow vegetable seeds in the vegetable seedling raising matrix according to claim 5 or 6 or in the vegetable seedling raising matrix prepared by the preparation method according to claim 7 or 8.

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