Method for producing acetic acid by using pigeon dung
By using pigeon manure as a nitrogen source and fermenting acetic acid bacteria for fermentation, the problems of environmental pollution and high cost of existing acetic acid production methods have been solved, and efficient and environmentally friendly acetic acid production has been achieved.
Patent Information
- Application Number
- CN202411752800.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-02
- Publication Date
- 2025-02-25
AI Technical Summary
The existing acetic acid production methods mainly rely on petrochemical routes, resulting in environmental pollution; while the acetic acid production of microbial fermentation methods is low and has high cost, which limits its application.
Pigeon manure is used as the nitrogen source and fermented with acetic acid bacteria to produce acetic acid, replacing traditional yeast powder as the nitrogen source.
It significantly increases the production of acetic acid, reduces production costs, and realizes the high-value utilization and resource recycling of pigeon manure, which has environmentally friendly economic benefits.
Smart Images
Figure CN119506366A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of feces biomass resource utilization, and specifically relates to a method for producing acetic acid by utilizing pigeon feces. Technical Background
[0002] Acetic acid, also known as ethanoic acid, is an important compound in the chemical industry and has a wide range of applications in pharmaceuticals, plastics, perfumes, coatings and textiles. At the same time, acetic acid is also a flavoring agent and preservative in food and beverages. my country is a major producer of acetic acid, with an annual output of more than 8 million tons. It is reported that the global demand for acetic acid is expected to rise from 16.3 million tons in 2018 to 20.3 million tons in 2024. At present, the production methods of acetic acid include methanol carbonylation, gas phase photocatalysis and hydrocarbon oxidation. However, these technologies mainly rely on petrochemical routes, which brings serious environmental problems. At present, microbial fermentation is a green and sustainable method for acetic acid production, which has received increasing attention.
[0003] Researchers isolated and screened acetic acid bacteria that can convert ethanol into acetic acid from different bacterial environments, including Gluconacetobacter, Gluconobacter, Acetobacter and Saccharomyces cerevisiae. However, factors such as low acetic acid production and high cost limit the production of acetic acid by microbial fermentation and its application. During the fermentation process, the choice of nitrogen source has an important impact on the growth rate, bacterial type formation and dissolved oxygen level of different microorganisms, as well as production costs. With the rapid development of livestock and poultry farming, the output of livestock and poultry manure continues to increase. According to statistics, the national meat pigeon farming industry has developed rapidly, increasing at an annual rate of 10% to 15%. At the same time, the total number of meat pigeons raised in my country accounts for more than 80% of the world, and the corresponding annual output of pigeon manure is huge. Pigeon manure has a high nitrogen content, is easy to corrupt and produces a foul odor, and also contains pathogens and parasite eggs that spread diseases and endanger health. On the other hand, pigeon manure is rich in organic matter, with a crude protein content of 21.8% to 28%, and contains nitrogen, phosphorus, potassium and a small amount of trace elements. Therefore, pigeon manure has the potential to be used as a nitrogen source in the acetic acid fermentation process. Summary of the invention
[0004] The purpose of the present invention is to provide a method for producing acetic acid using pigeon manure. The method is to replace the nitrogen source in the culture medium for producing acetic acid with pigeon manure from a breeding farm, and then inoculate acetic acid bacteria to produce acetic acid, thereby achieving the effect of reducing production costs and utilizing pigeon manure in a high-value manner.
[0005] The use of acetic acid bacteria to ferment pigeon manure to produce acetic acid not only has the potential to reduce the production cost of acetic acid and utilize pigeon manure in a high-value manner, but also can achieve the added value and efficiency of pigeon manure in terms of environment and economy. Through the search of relevant literature, it is found that there is no report on the research on the production of acetic acid by fermentation of pigeon manure at home and abroad.
[0006] To achieve the above purpose, the technical solution adopted by the present invention is as follows:
[0007] Acetic acid bacteria are inoculated into a fermentation medium with pigeon droppings as a nitrogen source for fermentation and cultivation to produce acetic acid.
[0008] Preferably, the acetic acid bacteria seed solution is inoculated into a fermentation medium with pigeon droppings as a nitrogen source at an inoculation rate of 4-10%, and cultured at 30° C. and 150-200 rpm for 20-30 hours to produce acetic acid by fermentation.
[0009] Preferably, the pigeon droppings are air-dried pigeon droppings.
[0010] Preferably, the acetic acid bacteria is Acetobacter pasteurianus.
[0011] Preferably, the fermentation medium using pigeon droppings as a nitrogen source comprises, by mass fraction, 3-8% glucose, 10-45 g / L pigeon droppings, 3% anhydrous ethanol (v / v), and a pH of 6-8.
[0012] Preferably, the fermentation medium using pigeon droppings as a nitrogen source comprises, by mass fraction, 3-8% glucose, 40 g / L pigeon droppings, and 3% anhydrous ethanol (v / v), and a pH of 6-8.
[0013] Preferably, the acetic acid bacteria seed solution is prepared by streaking acetic acid bacteria on a solid culture medium, culturing at 30° C. for 2 to 3 days to form colonies; picking up colonies and placing them in a liquid culture medium, culturing at 30° C. and 150 to 200 rpm for 16 to 24 hours;
[0014] Liquid culture medium: by mass fraction, including 0.5-1% glucose, 1% yeast powder, 3% anhydrous ethanol (v / v), pH 6.8;
[0015] Solid culture medium: by mass fraction, including 0.5-1% glucose, 1% yeast powder, 1-2% agar powder, 3% anhydrous ethanol (v / v), pH 6.8.
[0016] Beneficial effects:
[0017] 1. Using pigeon manure to replace the organic nitrogen source in the acetic acid production culture medium significantly increased the acetic acid yield and reduced the production cost of acetic acid.
[0018] 2. The present invention effectively achieves the reduction of pigeon manure, the increase of value and the improvement of efficiency, which is not only environmentally friendly, but also broadens the application field of pigeon manure and promotes its resource recycling. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 This is a comparison of the effects of pigeon droppings and yeast powder on the production of acetic acid by acetic acid bacteria fermentation.
[0020] Figure 2 It is the effect of pigeon manure concentration on the production of acetic acid by acetic acid bacteria.
[0021] Figure 3 It is the ratio of total organic acid content to acetic acid. DETAILED DESCRIPTION
[0022] The embodiments are provided to further explain and illustrate the present invention, rather than to limit the present invention.
[0023] The present invention is applicable to acetic acid bacteria strains, which can be obtained by screening or purchasing. The following examples are described by taking Acetobacter pasteurianus BNCC362975 as an example. The strain was purchased from the Henan Industrial Microbial Strain Engineering Technology Research Center.
[0024] Example 1 Effect of pigeon droppings and yeast powder on acetic acid production by acetic acid bacteria fermentation
[0025] The pigeon droppings were naturally air-dried. Take Acetobacter pasteurianus BNCC362975, streak it on a solid culture medium, and culture it at 30°C for 2 days to activate the bacteria and form a single colony. Then pick the colony, transfer it to a liquid culture medium, and culture it at 30°C and 180rpm for 18 hours to obtain a seed solution. The seed solution was inoculated into a yeast powder fermentation medium, a fermentation medium without yeast powder, and a fermentation medium containing only pigeon droppings (20g / L) as a nitrogen source at an inoculation rate of 5% by volume, and cultured at 30°C and 180rpm for 24 hours. The results showed that the acetic acid production of Acetobacter pasteurianus BNCC362975 in a nitrogen-free culture medium was only 10.8g / L, and the acetic acid production under the action of yeast powder (10g / L) reached 51.4g / L, while the acetic acid production produced by the strain under the condition of pigeon droppings (20g / L) as a nitrogen source was 52g / L ( Figure 1 ). This indicates that pigeon droppings and yeast powder have similar effects on the fermentation process of acetic acid production by Acetobacter pasteurianus BNCC362975.
[0026] Liquid culture medium: by mass fraction, including 1% glucose, 1% yeast powder, pH 6.8, sterilized at 115°C for 20 min, cooled to 50°C and added with 3% anhydrous ethanol (v / v).
[0027] Solid culture medium: by mass fraction, including 1% glucose, 1% yeast powder, 1.5% agar powder, pH 6.8, sterilized at 115°C for 20 min, cooled to 50°C and added with 3% anhydrous ethanol (v / v).
[0028] Yeast powder fermentation medium: by mass fraction, including 5% glucose, 1% yeast powder, solvent is water, pH is 6.8, mix all ingredients evenly, sterilize at 115℃ for 20min, cool to 50℃ and add 3% anhydrous ethanol (v / v). The fermentation medium does not contain yeast powder, and the rest is the same as the yeast powder fermentation medium.
[0029] Pigeon manure fermentation medium: by mass fraction, including 5% glucose, pigeon manure (20g / L), solvent is water, pH is 6.8, all ingredients are mixed evenly, sterilized at 115°C for 20min, cooled to 50°C and added with 3% anhydrous ethanol (v / v).
[0030] Example 2 Effect of using pigeon droppings instead of yeast powder on acetic acid production by acetic acid bacteria
[0031] Pigeon manure has achieved the same effect as yeast powder in fermenting acetic acid bacteria to produce acetic acid. Pigeon manure can be used as a nitrogen source to replace yeast powder for acetic acid fermentation. The concentration of pigeon manure (10, 15, 20, 25, 30, 35, 40, 45 g / L) was further optimized. Figure 2 As shown in the figure, with the continuous increase of pigeon manure concentration, the production of acetic acid produced by Acetobacter pasteurianus BNCC362975 using pigeon manure first increased and then decreased. When the concentration of pigeon manure was 40g / L, the acetic acid production reached the highest level, which was 59.4g / L. This shows that using pigeon manure as a nitrogen source significantly increased the production of acetic acid by acetic acid bacteria fermentation. This may be because the nitrogen-containing substances in pigeon manure provide an available nitrogen source for the growth and metabolism of acetic acid bacteria to produce acetic acid. At the same time, nitrogen, phosphorus, potassium and trace elements in pigeon manure may also promote the production of acetic acid.
[0032] Example 3 Effect of different pigeon manure concentrations on the production of total organic acids by acetic acid bacteria
[0033] In order to explore the difference between pigeon manure and yeast powder in the fermentation process of acetic acid bacteria, the content of total organic acid during their fermentation for 24 hours was compared (the fermentation method was the same as in Example 1). Figure 3As shown, the total organic acid content of Acetobacter pasteurianus BNCC362975 in nitrogen-free medium is the lowest, and the total organic acid content in the medium containing yeast powder reaches 75.4g / L; the total organic acid content in the medium containing different pigeon manure concentrations first increases and then decreases, reaching a maximum of 89.9g / L when the pigeon manure concentration is 35g / L. On the other hand, the proportion of acetic acid in total organic acids in yeast powder medium of this strain (68.1%) is significantly higher than that in nitrogen-free medium (59.6%). With the increase of pigeon manure concentration, the proportion of acetic acid fluctuates, which is due to the increase or decrease of other organic acid components leading to the change of the proportion of acetic acid. When the concentration of pigeon manure is 40g / L, the proportion of acetic acid reaches 71%; at this time, the acetic acid production is the highest ( Figure 2 Although, when the concentration of pigeon manure was low (<15 g / L), the proportion of acetic acid was high (>72%), the total organic acid production was low, and the acetic acid production was also low, which is consistent with Figure 2 The results shown are consistent.
[0034] In summary, replacing yeast powder with pigeon manure can significantly increase the production of acetic acid and its proportion in total organic acids.
[0035] Detection of organic acid production: The fermentation broth was centrifuged (8000 rpm, 10 min) and the supernatant was taken; after appropriate dilution, the concentration of organic acids in the supernatant was detected by high performance liquid chromatography, and the organic acids included acetic acid, formic acid, tartaric acid, citric acid, malic acid, succinic acid, lactic acid and fumaric acid.
[0036] The above embodiments are merely exemplary of the present invention, and the protection scope of the present invention is not limited thereto. Any person familiar with the technical field can obtain simple changes or combination changes of the formula, simple changes or equivalent replacements of the technical solutions within the technical scope disclosed by the present invention, which all belong to the protection scope of the present invention.
Claims
1. A method for producing acetic acid using pigeon droppings, characterized in that: Acetic acid bacteria are inoculated into a fermentation medium with pigeon droppings as a nitrogen source for fermentation and cultivation to produce acetic acid.
2. The method for producing acetic acid using pigeon droppings according to claim 1, characterized in that: The method is to inoculate acetic acid bacteria seed liquid into a fermentation medium with pigeon droppings as a nitrogen source at an inoculation amount of 4-10%, culture it at 30°C and 150-200rpm for 20-30h, and ferment and culture to produce acetic acid.
3. The method for producing acetic acid using pigeon droppings according to claim 1, characterized in that: The pigeon droppings are air-dried pigeon droppings.
4. The method for producing acetic acid using pigeon droppings according to claim 1, characterized in that: The acetic acid bacteria is Acetobacter pasteurianus.
5. The method for producing acetic acid using pigeon droppings according to claim 1, characterized in that: The fermentation medium using pigeon droppings as a nitrogen source comprises, by mass fraction, 3-8% glucose, 10-45g / L pigeon droppings, 3% anhydrous ethanol (v / v), and a pH value of 6-8.
6. The method for producing acetic acid using pigeon droppings according to claim 5, characterized in that: The fermentation medium using pigeon droppings as a nitrogen source comprises, by mass fraction, 3-8% glucose, 40 g / L pigeon droppings, and 3% anhydrous ethanol (v / v). The pH is 6-8.
7. The method for producing acetic acid using pigeon droppings according to claim 2, characterized in that: The acetic acid bacteria seed liquid is obtained by streaking acetic acid bacteria on a solid culture medium, culturing at 30° C. for 2 to 3 days to form colonies; picking up colonies and placing them in a liquid culture medium, culturing at 30° C. and 150 to 200 rpm for 16 to 24 hours; Liquid culture medium: by mass fraction, including 0.5-1% glucose, 1% yeast powder, 3% anhydrous ethanol (v / v), pH 6.8; Solid culture medium: by mass fraction, including 0.5-1% glucose, 1% yeast powder, 1-2% agar powder, 3% anhydrous ethanol (v / v), pH 6.8.