Tracing detection method and equipment for biochemical oxygen-consuming organic matters in water environment

Abstract

The invention provides a traceability detection method and equipment for biochemical oxygen-consuming organic matters in a water environment. The method comprises the following steps: collecting a water body sample of a to-be-detected environment; obtaining a first carbon stable isotope composition characteristic and a first substance amount concentration of the water body sample under a first condition, and a second carbon stable isotope composition characteristic and a second substance amount concentration of the water body sample under a second condition; performing inversion calculation on the first carbon stable isotope composition characteristic, the first substance amount concentration, the second carbon stable isotope composition characteristic and the second substance amount concentration by adopting a molar mass balance equation of a carbon element to obtain a carbon stable isotope composition characteristic calculation value of the biochemical oxygen-consuming organic matter of the environment to be detected; according to the carbon stable isotope composition characteristic calculation value of the biochemical oxygen-consuming organic matter of the environment to be detected, comparison processing is performed in the pre-established carbon stable isotope composition characteristic fingerprint database to obtain corresponding source data, so that the technical effect of improving the traceability detection efficiency is achieved.

Description

Technical Field

[0001] The present application relates to the technical field of water environment detection, and in particular to a method and equipment for tracing the source of biochemical oxygen-consuming organic matter in a water environment. Background Art

[0002] With the continuous progress of science and technology and society, environmental protection has increasingly become the focus of public attention. Among them, the control of biochemical oxygen-consuming organic matter is particularly critical. Biochemical oxygen-consuming organic matter refers to organic pollutants that exist in environmental water bodies, are easily degraded by microorganisms and consume oxygen. High concentrations of biochemical oxygen-consuming organic matter can cause hypoxia in water bodies, becoming one of the main culprits causing the frequent occurrence of black and smelly water bodies. Therefore, tracing the source of biochemical oxygen-consuming organic matter and implementing effective control measures on this basis are crucial to improving the hypoxia of environmental water bodies and eliminating black and smelly water bodies. Therefore, the traceability detection method of biochemical oxygen-consuming organic matter in water environments has become a direction with application prospects.

[0003] In the prior art, the main methods used for traceability detection of biochemical oxygen-consuming organic matter in water environments include traditional chemical analysis, spectral analysis, and microbial community analysis, which are independently applied in the traceability detection process.

[0004] Organic matter in environmental water bodies can be divided into three types according to their biodegradation characteristics: readily biodegradable (i.e., biochemically oxygen-consuming organic matter), slowly biodegradable, and inert. However, the above-mentioned existing technologies cannot effectively distinguish the above-mentioned types of organic matter, and the overall traceability detection process is highly complex, which in turn leads to the consumption of a large amount of material and time resources, and there is a technical problem of low efficiency of traceability detection. Summary of the invention

[0005] The present application provides a method and device for tracing the source of biochemical oxygen-consuming organic matter in an aquatic environment, so as to achieve the technical effect of improving the efficiency of tracing the source of detection.

[0006] In a first aspect, the present application provides a method for tracing the source of biochemical oxygen-consuming organic matter in an aquatic environment, comprising:

[0007] Collect water samples from the environment to be tested;

[0008] Obtaining a first carbon stable isotope composition characteristic and a first substance concentration of a water sample under a first condition, and a second carbon stable isotope composition characteristic and a second substance concentration of the water sample under a second condition, wherein the first condition is before an aerobic degradation culture experiment of the environmental water sample, and the second condition is after an aerobic degradation culture experiment of the environmental water sample;

[0009] Using the molar mass balance equation of carbon element, the first carbon stable isotope composition characteristic, the molar concentration of the first substance, the second carbon stable isotope composition characteristic and the molar concentration of the second substance are inverted and calculated to obtain the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected;

[0010] According to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, a comparison process is performed in a pre-established carbon stable isotope composition characteristic fingerprint database to obtain corresponding source data, wherein the source data is the source data of the biochemical oxygen-consuming organic matter in the environment to be detected.

[0011] Optionally, according to the calculated carbon stable isotope composition characteristic value of the biochemical oxygen-consuming organic matter in the environment to be detected, a comparison process is performed in a pre-established carbon stable isotope composition characteristic fingerprint database to obtain corresponding source data, including:

[0012] According to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, a comparison process is performed in a pre-established carbon stable isotope composition characteristic fingerprint database to determine at least one target source type of the biochemical oxygen-consuming organic matter in the environment to be detected;

[0013] According to at least one target source type, the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be detected is determined.

[0014] Optionally, according to at least one target source type, determining the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be detected includes:

[0015] If there are multiple target source types, the average value and standard deviation of the carbon stable isotope composition characteristics of the target source types are obtained;

[0016] Using the calculated value of carbon stable isotope composition characteristics, the average value and standard deviation of carbon stable isotope composition characteristics of target source types as prior information and the chemical mass balance formula as constraints, the Markov Chain Monte Carlo algorithm is used to calculate the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be tested.

[0017] Optionally, before collecting the water sample of the environment to be tested, the method further includes:

[0018] Access historical environmental data;

[0019] A carbon stable isotope composition characteristic fingerprint database is established based on the historical environmental data corresponding to the preset source types, wherein the source types include at least one of domestic sewage, organic industrial wastewater, C3 plant debris, C4 plant debris, phytoplankton residues, and soil.

[0020] Optionally, obtaining a first carbon stable isotope composition characteristic and a first substance concentration of a water sample under a first condition includes:

[0021] The dissolved inorganic carbon in the water sample under the first condition is extracted by chemical precipitation;

[0022] A first carbon stable isotope composition characteristic and a first molar concentration of dissolved inorganic carbon under a first condition are obtained.

[0023] Alternatively, the molar mass balance equation is:

[0024]

[0025] in, is the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be tested, It is the second carbon stable isotope composition characteristic. is the concentration of the second substance, It is the first carbon stable isotope composition characteristic. is the concentration of the first substance.

[0026] Optionally, after performing a comparison process in a pre-established carbon stable isotope composition characteristic fingerprint database according to the calculated carbon stable isotope composition characteristic value of the biochemical oxygen-consuming organic matter in the environment to be detected to obtain corresponding source data, the method further includes:

[0027] The source data of the biochemical oxygen-consuming organic matter in the environment to be detected is sent to the user terminal, so that the source data of the biochemical oxygen-consuming organic matter in the environment to be detected is displayed on the user terminal.

[0028] In a second aspect, the present application provides a device for tracing the source of biochemical oxygen-consuming organic matter in a water environment, comprising:

[0029] A collection module, used to collect water samples from the environment to be tested;

[0030] An acquisition module, used to acquire a first carbon stable isotope composition characteristic and a first substance concentration of a water sample under a first condition, and a second carbon stable isotope composition characteristic and a second substance concentration of the water sample under a second condition, wherein the first condition is before an aerobic degradation culture experiment of the environmental water sample, and the second condition is after an aerobic degradation culture experiment of the environmental water sample;

[0031] The first processing module is used to use the molar mass balance equation of carbon element to perform inverse calculation on the first carbon stable isotope composition characteristic, the molar concentration of the first substance, the second carbon stable isotope composition characteristic and the molar concentration of the second substance to obtain the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected;

[0032] The second processing module is used to compare and process the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected in a pre-established carbon stable isotope composition characteristic fingerprint database to obtain corresponding source data, wherein the source data is the source data of the biochemical oxygen-consuming organic matter in the environment to be detected.

[0033] Optionally, the second processing module is further used for:

[0034] According to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, a comparison process is performed in a pre-established carbon stable isotope composition characteristic fingerprint database to determine at least one target source type of the biochemical oxygen-consuming organic matter in the environment to be detected;

[0035] According to at least one target source type, the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be detected is determined.

[0036] Optionally, the second processing module is further used for:

[0037] If there are multiple target source types, the average value and standard deviation of the carbon stable isotope composition characteristics of the target source types are obtained;

[0038] Using the calculated value of carbon stable isotope composition characteristics, the average value and standard deviation of carbon stable isotope composition characteristics of target source types as prior information and the chemical mass balance formula as constraints, the Markov Chain Monte Carlo algorithm is used to calculate the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be tested.

[0039] Optionally, the acquisition module is further used for:

[0040] Access historical environmental data;

[0041] A carbon stable isotope composition characteristic fingerprint database is established based on the historical environmental data corresponding to the preset source types, wherein the source types include at least one of domestic sewage, organic industrial wastewater, C3 plant debris, C4 plant debris, phytoplankton residues, and soil.

[0042] Optionally, the acquisition module is also used to:

[0043] The dissolved inorganic carbon in the water sample under the first condition is extracted by chemical precipitation;

[0044] A first carbon stable isotope composition characteristic and a first molar concentration of dissolved inorganic carbon under a first condition are obtained.

[0045] Optionally, the first processing module is also used for a molar mass balance equation, which is:

[0046]

[0047] in, is the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be tested, It is the second carbon stable isotope composition characteristic. is the concentration of the second substance, It is the first carbon stable isotope composition characteristic. is the concentration of the first substance.

[0048] Optionally, the second processing module is further used for:

[0049] The source data of the biochemical oxygen-consuming organic matter in the environment to be detected is sent to the user terminal, so that the source data of the biochemical oxygen-consuming organic matter in the environment to be detected is displayed on the user terminal.

[0050] In a third aspect, the present application provides a traceability detection device for biochemical oxygen-consuming organic matter in a water environment, comprising:

[0051] at least one processor; and

[0052] a memory communicatively connected to at least one processor; wherein,

[0053] The memory stores instructions that can be executed by at least one processor, and the instructions are executed by at least one processor so that the at least one processor can execute various possible implementations of the first aspect as described above.

[0054] In a fourth aspect, the present application provides a computer-readable storage medium, in which computer-executable instructions are stored. When the computer-executable instructions are executed by a processor, they are used to implement various possible implementation methods as in the first aspect.

[0055] In a fifth aspect, the present application provides a computer program product, which, when executed by a processor, is used to implement various possible implementations as in the first aspect.

[0056] The present application provides a method and device for tracing the source of biochemical oxygen-consuming organic matter in aquatic environment, which collects a water sample from the environment to be detected; obtains a first carbon stable isotope composition characteristic and a first substance molar concentration of the water sample under a first condition, and a second carbon stable isotope composition characteristic and a second substance molar concentration of the water sample under a second condition; uses a molar mass balance equation of the carbon element to inversely calculate the first carbon stable isotope composition characteristic, the first substance molar concentration, the second carbon stable isotope composition characteristic and the second substance molar concentration to obtain a calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected; performs a comparison process in a pre-established carbon stable isotope composition characteristic fingerprint database based on the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, and obtains a fingerprint of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected. The corresponding source data is obtained, and based on the principle that "the microbial aerobic degradation process of organic matter will not cause obvious fractionation effect between the carbon stable isotopes of reactants and products", the traceability of biochemical oxygen-consuming organic matter is realized. Therefore, the carbon stable isotope composition characteristics and the molar concentration of the water sample are inverted by using the molar mass balance equation of the carbon element to obtain the corresponding characteristic calculation values, thereby ensuring the accuracy and reliability of the subsequent traceability results. At the same time, by comparing the pre-established carbon stable isotope composition characteristic fingerprint database with the characteristic calculation values, the convenience and speed of the traceability detection process are improved, the complexity of the traceability operation is reduced, the waste of time cost is avoided, the technical problem of low efficiency of traceability detection of biochemical oxygen-consuming organic matter in the water environment is solved, and the technical effect of improving the efficiency of traceability detection is achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0058] Figure 1 The process of the traceability detection method of biochemical oxygen-consuming organic matter in water environment provided in the embodiment of the present application Figure 1 ;

[0059] Figure 2 The process of the traceability detection method of biochemical oxygen-consuming organic matter in water environment provided in the embodiment of the present application Figure 2 ;

[0060] Figure 3 The process of the traceability detection method of biochemical oxygen-consuming organic matter in water environment provided in the embodiment of the present application Figure 3 ;

[0061] Figure 4 A schematic diagram of the structure of a device for tracing the source of biochemical oxygen-consuming organic matter in a water environment provided in an embodiment of the present application;

[0062] Figure 5 This is a hardware structure diagram of the traceability detection equipment for biochemical oxygen-consuming organic matter in a water environment provided in an embodiment of the present application.

[0063] The above drawings have shown clear embodiments of the present application, which will be described in more detail later. These drawings and text descriptions are not intended to limit the scope of the present application in any way, but to illustrate the concept of the present application to those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION

[0064] Exemplary embodiments will be described in detail herein, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The implementations described in the following exemplary embodiments do not represent all implementations consistent with the present application. Instead, they are merely examples of devices and methods consistent with some aspects of the present application as detailed in the appended claims.

[0065] In the prior art, the traceability detection method cannot effectively distinguish the above-mentioned types of organic matter, and the overall traceability detection process is highly complicated, which leads to the consumption of a large amount of material and time resources, and there is a technical problem of low efficiency of traceability detection.

[0066] The present application provides a method and electronic equipment for tracing the source of biochemical oxygen-consuming organic matter in an aquatic environment. Based on the principle that "the microbial aerobic degradation process of organic matter will not cause an obvious fractionation effect between the carbon stable isotopes of reactants and products", the molar mass balance equation of the carbon element is used to inversely calculate the carbon stable isotope composition characteristics and the molar concentration of the water sample to obtain the corresponding characteristic calculation value, thereby ensuring the accuracy and reliability of the subsequent tracing results. At the same time, by comparing the pre-established carbon stable isotope composition characteristic fingerprint database with the characteristic calculation value, not only the tracing of the biochemical oxygen-consuming organic matter is achieved, but also the convenience and speed of the tracing detection process are improved, the complexity of the tracing operation is reduced, the waste of time cost is avoided, the technical problem of low efficiency of tracing detection of biochemical oxygen-consuming organic matter in an aquatic environment is solved, and the technical effect of improving the efficiency of tracing detection is achieved.

[0067] The technical solution of the present application and how the technical solution of the present application solves the above-mentioned technical problems are described in detail below with specific embodiments. The following specific embodiments can be combined with each other, and the same or similar concepts or processes may not be repeated in some embodiments. The embodiments of the present application will be described below in conjunction with the accompanying drawings.

[0068] Figure 1 The process of the traceability detection method of biochemical oxygen-consuming organic matter in water environment provided in the embodiment of the present application Figure 1.like Figure 1 As shown, the method for tracing the source of biochemical oxygen-consuming organic matter in a water environment provided in the embodiment of the present application includes:

[0069] S101, collecting water samples from the environment to be tested;

[0070] In this embodiment, the water sample refers to a water sample collected from the environment to be tested and used to analyze the source of biochemical oxygen-consuming organic matter.

[0071] Specifically, when collecting water samples from the environment to be tested, a stainless steel sampler is used to avoid contamination of the water samples. When collecting water samples, a representative location should be selected, such as a certain depth below the river surface (such as 35 cm) to ensure that the collected water samples can truly reflect the water quality of the environment to be tested. After the collection is completed, the water sample must be immediately transferred to a pre-treated brown glass sampling bottle using the catheter overflow method and sealed to prevent the water sample from being contaminated or changed during transportation and storage. The sampling bottle should be soaked in 1 mol / L HCl for 24 hours and dried in advance to remove possible residual impurities and moisture. The collected water samples should be transported back to the laboratory as soon as possible and stored at 4°C for subsequent analysis and testing.

[0072] S102, obtaining a first carbon stable isotope composition characteristic and a first substance concentration of the water sample under a first condition, and a second carbon stable isotope composition characteristic and a second substance concentration of the water sample under a second condition;

[0073] In this embodiment, the first condition is before the aerobic degradation culture experiment of the environmental water sample, and the second condition is after the aerobic degradation culture experiment of the environmental water sample.

[0074] Specifically, when obtaining the above parameters, strict experimental steps and operating procedures need to be followed: First, before the experiment begins, an appropriate extraction method (such as chemical precipitation, acidification ultrasound, etc.) is required to extract the dissolved inorganic carbon in the system, and a stable isotope ratio mass spectrometer is used to determine the corresponding first carbon stable isotope composition characteristics and the first substance concentration. Then, referring to the "Dilution and Inoculation Method for Determination of Five-Day Biochemical Oxygen Demand of Water Quality (HJ 505-2009)", an aerobic degradation culture experiment of environmental water samples is carried out under the conditions of constant temperature, light avoidance, sealing and adding nitrification reaction inhibition. After the experiment, the above extraction and determination method is also used to obtain the second carbon stable isotope composition characteristics and the second substance concentration of the dissolved inorganic carbon under the second conditions. The test results of the above parameters are shown in the following table. It can be understood that Table 1 is only a schematic test result and does not affect the protection scope of the embodiments of the present application.

[0075] Table 1 Detection values ​​of system parameters during aerobic culture

[0076]

[0077] S103, using the molar mass balance equation of carbon element, inversely calculating the first carbon stable isotope composition characteristic, the molar concentration of the first substance, the second carbon stable isotope composition characteristic, and the molar concentration of the second substance, to obtain a calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected;

[0078] In this embodiment, the molar mass balance equation is:

[0079]

[0080] in, is the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be tested, It is the second carbon stable isotope composition characteristic. is the concentration of the second substance, It is the first carbon stable isotope composition characteristic. is the concentration of the first substance.

[0081] Based on the principle of carbon conservation, the carbon stable isotope composition characteristics and the changes in the molar concentration before and after the aerobic degradation culture experiment were compared to invert the calculated value of the carbon stable isotope composition characteristics of the biochemical aerobic organic matter.

[0082] S104, performing a comparison process in a pre-established carbon stable isotope composition characteristic fingerprint database according to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, and obtaining corresponding source data.

[0083] In this embodiment, the source data is the source data of biochemical oxygen-consuming organic matter in the environment to be detected.

[0084] Specifically, after obtaining the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be tested, it is necessary to compare and analyze it with the pre-constructed carbon stable isotope composition characteristic database of potential sources of biochemical oxygen-consuming organic matter. The database contains the carbon stable isotope composition characteristic values ​​of various possible potential sources of biochemical oxygen-consuming organic matter (such as domestic sewage, organic industrial wastewater, C3 plant debris, C4 plant debris, phytoplankton remains, soil, etc.). Through comparison and analysis, the corresponding source data of the biochemical oxygen-consuming organic matter in the environment to be tested can be determined.

[0085] The present application provides a method for tracing the source of biochemical oxygen-consuming organic matter in aquatic environment, which comprises collecting a water sample of the environment to be detected; obtaining a first carbon stable isotope composition characteristic and a first substance molar concentration of the water sample under a first condition, and a second carbon stable isotope composition characteristic and a second substance molar concentration of the water sample under a second condition; using a molar mass balance equation of the carbon element, inversely calculating the first carbon stable isotope composition characteristic, the first substance molar concentration, the second carbon stable isotope composition characteristic and the second substance molar concentration to obtain a calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected; and obtaining a calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected based on the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected. The established carbon stable isotope composition characteristic fingerprint database is used for comparison processing to obtain the corresponding source data, and then the carbon stable isotope composition characteristics and the amount of substance concentration of the water sample are inverted by using the molar mass balance equation of the carbon element to obtain the corresponding characteristic calculation value, thereby ensuring the accuracy and reliability of the subsequent traceability results. At the same time, by comparing the pre-established carbon stable isotope composition characteristic fingerprint database with the characteristic calculation value, the convenience and speed of the traceability detection process are improved, the complexity of the traceability operation is reduced, the waste of time cost is avoided, the technical problem of low efficiency of traceability detection of biochemical oxygen-consuming organic matter in the water environment is solved, and the technical effect of improving the efficiency of traceability detection is achieved.

[0086] Figure 2 The process of the traceability detection method of biochemical oxygen-consuming organic matter in water environment provided in the embodiment of the present application Figure 2 .like Figure 2 As shown, this embodiment provides a supplementary explanation of the process of obtaining source data based on the above embodiment, including:

[0087] S201, performing a comparison process in a pre-established carbon stable isotope composition characteristic fingerprint database according to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, so as to determine at least one target source type of the biochemical oxygen-consuming organic matter in the environment to be detected;

[0088] The calculated value of the carbon stable isotope composition characteristics of the biochemical oxygen-consuming organic matter in the environment to be tested obtained by inversion calculation is compared with the pre-constructed carbon stable isotope composition characteristic fingerprint database. Through the comparison, one or more possible target source types of the biochemical oxygen-consuming organic matter in the water body of the environment to be tested are determined.

[0089] Optionally, when there is only one target source type of biochemical oxygen-consuming organic matter in the environmental water body, it is determined that all of the biochemical oxygen-consuming organic matter in the environmental water body originates from the target source type.

[0090] S202. If there are multiple target source types, obtain the average value and standard deviation of the carbon stable isotope composition characteristics of the target source types;

[0091] When it is determined that there are multiple source types of biochemical oxygen-consuming organic matter in the environment to be detected, the average value and standard deviation of the carbon stable isotope composition characteristics of each target source type are extracted from a pre-established carbon stable isotope composition characteristic fingerprint database.

[0092] S203. Using the calculated value of carbon stable isotope composition characteristics, the average value and standard deviation of carbon stable isotope composition characteristics of target source types as prior information and the chemical mass balance formula as a constraint, a Markov chain Monte Carlo algorithm is used to calculate the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be tested.

[0093] In this embodiment, the chemical mass balance formula is:

[0094]

[0095] Among them, δ 13 C BOM is the δ1 of oxygen-consuming organic matter in environmental water 13 C numerical value; is the δ of oxygen-consuming organic matter of the kth source type 13 C numerical value; P k is the contribution rate of oxygen-consuming organic matter of the kth source type; is the residual, which represents the error value that is not effectively fitted.

[0096] The calculated carbon stable isotope composition characteristic values, the average values ​​and standard deviations of the carbon stable isotope composition characteristics of the target source types are used as prior information, constraints are established according to the chemical equilibrium formula, and the Markov Chain Monte Carlo algorithm is used to calculate the composition ratios of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be tested, combining the prior information and constraints.

[0097] Specifically, when there are multiple sources of biochemical oxygen-consuming organic matter (K≥2), the biochemical oxygen-consuming organic matter of each source type is obtained. The average value of (k=1,..., K) ) and standard deviation ( ); using the inverted δ 13 C BOM Data, biochemical oxygen-consuming organic matter by source type The average value ( ) and standard deviation ( ) data as prior information, and the above chemical mass balance formula as a constraint, the Markov chain Monte Carlo algorithm is used to generate a large number of contribution ratios that meet the constraint conditions ( ) is the posterior distribution of The average value of the posterior distribution is taken as the final quantitative result of the contribution rate of biochemical oxygen-consuming organic matter from each source type to the biochemical oxygen-consuming organic matter in environmental water bodies.

[0098] The present application provides a method for tracing the source of biochemical oxygen-consuming organic matter in a water environment. Through the Markov chain Monte Carlo algorithm, the accuracy of the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be detected is improved, the stability of the traceability detection is ensured, and the technical problem of low efficiency of traceability detection of biochemical oxygen-consuming organic matter in a water environment is solved, thereby achieving the technical effect of improving the efficiency of traceability detection.

[0099] Figure 3 The process of the traceability detection method of biochemical oxygen-consuming organic matter in water environment provided in the embodiment of the present application Figure 3 .like Figure 3 As shown, this embodiment, based on the above embodiment, provides a supplementary description of the source tracing detection method of biochemical oxygen-consuming organic matter in a water environment, including:

[0100] S301, acquiring historical environmental data, and establishing a carbon stable isotope composition characteristic fingerprint database according to the historical environmental data corresponding to the preset source type;

[0101] In this embodiment, the source type includes at least one of domestic sewage, organic industrial wastewater, C3 plant debris, C4 plant debris, phytoplankton residues, and soil.

[0102] According to the water environment where the water sample is located, the corresponding historical environmental data are obtained, and the historical environmental data corresponding to phytoplankton and domestic sewage are selected from these historical environmental data to establish a carbon stable isotope composition characteristic fingerprint database.

[0103] Specifically, domestic sewage-derived organic matter is mainly composed of protein (40-60%), carbohydrates (30-50%) and fat (10%), phytoplankton-derived organic matter is mainly composed of protein (36-70%) and carbohydrates, and C3 plant-derived organic matter is mainly composed of lignin, hemicellulose and cellulose. Compared with C3 plant-derived organic matter, domestic sewage-derived organic matter and phytoplankton-derived organic matter have higher content of hydrophilic components, lower content of aromatic groups, smaller molecular weight, and are easily degraded by microorganisms. Therefore, the potential sources of biochemical oxygen-consuming organic matter in river water bodies are domestic sewage and phytoplankton. Referring to relevant literature, the δ 13C value. It is understandable that Table 2 is only a schematic test result and does not affect the protection scope of the embodiments of the present application.

[0104] Table 2 δ of organic matter from domestic sewage and phytoplankton sources 13 C Value

[0105]

[0106] S302, extracting the dissolved inorganic carbon of the water sample under the first condition by chemical precipitation;

[0107] Specifically, under the first condition (i.e., before the start of the aerobic degradation culture experiment), the chemical precipitation method is used to extract the dissolved inorganic carbon in the water sample. The chemical precipitation method can precipitate the dissolved inorganic carbon in the form of precipitation by adding appropriate chemical reagents, and then obtain a pure dissolved inorganic carbon sample through filtering, washing and other steps.

[0108] S303, obtaining a first carbon stable isotope composition characteristic and a first substance concentration of dissolved inorganic carbon under a first condition;

[0109] The dissolved inorganic carbon obtained from the water sample under the first condition is detected to obtain a first carbon stable isotope composition characteristic and a first molar concentration of the dissolved inorganic carbon under the first condition.

[0110] S304, obtaining a second carbon stable isotope composition characteristic and a second substance concentration of the water sample under a second condition;

[0111] In this embodiment, the second carbon stable isotope composition characteristic and the molar concentration of the second substance are obtained in the same manner as the first carbon stable isotope composition characteristic and the molar concentration of the first substance, and are not described in detail herein.

[0112] The dissolved inorganic carbon obtained from the water sample under the second condition is detected to obtain the second carbon stable isotope composition characteristics and the second molar concentration of the dissolved inorganic carbon under the second condition.

[0113] S305, using the molar mass balance equation of carbon element, inversely calculating the first carbon stable isotope composition characteristic, the molar concentration of the first substance, the second carbon stable isotope composition characteristic, and the molar concentration of the second substance, to obtain a calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected;

[0114] Based on the principle of conservation of carbon, the molar mass balance equation of carbon is established. By using the carbon stable isotope composition characteristics and substance concentration data of soluble inorganic carbon under the first and second conditions, the calculated value of the carbon stable isotope composition characteristics of biochemical oxygen-consuming organic matter in the environment to be tested can be inverted.

[0115] S306, performing a comparison process in a pre-established carbon stable isotope composition characteristic fingerprint database according to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, and obtaining corresponding source data;

[0116] In this embodiment, the method for acquiring the source data is the same as that in the above-mentioned S104, and will not be described in detail here.

[0117] S307 , sending the source data of the biochemical oxygen-consuming organic matter in the environment to be detected to the user terminal, so that the source data of the biochemical oxygen-consuming organic matter in the environment to be detected is displayed on the user terminal.

[0118] After it is determined that the source data corresponding to the biochemical oxygen-consuming organic matter in the environment to be detected is obtained, it is sent to the user terminal. After the user terminal receives it, the source data of the biochemical oxygen-consuming organic matter in the environment to be detected is visualized.

[0119] The present application provides a method for tracing the source of biochemical oxygen-consuming organic matter in a water environment. By pre-establishing a carbon stable isotope composition characteristic fingerprint database, on the one hand, the source type and corresponding historical environmental data in the fingerprint database can be adjusted to be applicable to water bodies in different regions and with different pollution conditions, thereby ensuring the flexibility of the tracing detection. On the other hand, by comparing it with the characteristic calculated value, the convenience and rapidity of the tracing detection process are improved, the complexity of the tracing operation is reduced, and the waste of time cost is avoided. In addition, by extracting and detecting the carbon stable isotope composition characteristics and the molar concentration of dissolved inorganic carbon under the first condition and the second condition respectively, the comprehensiveness of the data is ensured, richer information is provided for subsequent tracing detection, the accuracy of subsequent tracing detection is improved, and the technical problem of low efficiency of tracing the source of biochemical oxygen-consuming organic matter in aquatic environments is solved, thereby achieving the technical effect of improving the efficiency of tracing detection.

[0120] Figure 4 This is a schematic diagram of the structure of the device for tracing the source of biochemical oxygen-consuming organic matter in a water environment provided in an embodiment of the present application. The device of this embodiment can be in the form of software and / or hardware. Figure 4 As shown, the source tracing detection device 400 for biochemical oxygen-consuming organic matter in a water environment provided in an embodiment of the present application includes: a collection module 401, an acquisition module 402, a first processing module 403, and a second processing module 404:

[0121] The collection module 401 is used to collect water samples from the environment to be tested;

[0122] An acquisition module 402 is used to acquire a first carbon stable isotope composition characteristic and a first substance concentration of a water sample under a first condition, and a second carbon stable isotope composition characteristic and a second substance concentration of the water sample under a second condition, wherein the first condition is before an aerobic degradation culture experiment of the environmental water sample, and the second condition is after an aerobic degradation culture experiment of the environmental water sample;

[0123] The first processing module 403 is used to use the molar mass balance equation of carbon element to perform inverse calculation on the first carbon stable isotope composition characteristic, the molar concentration of the first substance, the second carbon stable isotope composition characteristic and the molar concentration of the second substance to obtain the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected;

[0124] The second processing module 404 is used to perform comparison processing in a pre-established carbon stable isotope composition characteristic fingerprint database according to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, so as to obtain corresponding source data, wherein the source data is the source data of the biochemical oxygen-consuming organic matter in the environment to be detected.

[0125] In a possible implementation, the second processing module 404 is further configured to:

[0126] According to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, a comparison process is performed in a pre-established carbon stable isotope composition characteristic fingerprint database to determine at least one target source type of the biochemical oxygen-consuming organic matter in the environment to be detected;

[0127] According to at least one target source type, the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be detected is determined.

[0128] In a possible implementation, the second processing module 404 is further configured to:

[0129] If there are multiple target source types, the average value and standard deviation of the carbon stable isotope composition characteristics of the target source types are obtained;

[0130] Using the calculated value of carbon stable isotope composition characteristics, the average value and standard deviation of carbon stable isotope composition characteristics of target source types as prior information and the chemical mass balance formula as constraints, the Markov Chain Monte Carlo algorithm is used to calculate the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be tested.

[0131] In a possible implementation, the acquisition module 401 is further used for:

[0132] Access historical environmental data;

[0133] A carbon stable isotope composition characteristic fingerprint database is established based on the historical environmental data corresponding to the preset source types, where the source types include phytoplankton and domestic sewage.

[0134] In a possible implementation, the acquisition module 402 is further configured to:

[0135] The dissolved inorganic carbon in the water sample under the first condition is extracted by chemical precipitation;

[0136] A first carbon stable isotope composition characteristic and a first molar concentration of dissolved inorganic carbon under a first condition are obtained.

[0137] In a possible implementation, the first processing module 403 is also used for a molar mass balance equation, which is:

[0138]

[0139] in, is the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be tested, It is the second carbon stable isotope composition characteristic. is the concentration of the second substance, It is the first carbon stable isotope composition characteristic. is the concentration of the first substance.

[0140] In a possible implementation, the second processing module 404 is further configured to:

[0141] The source data of the biochemical oxygen-consuming organic matter in the environment to be detected is sent to the user terminal, so that the source data of the biochemical oxygen-consuming organic matter in the environment to be detected is displayed on the user terminal.

[0142] The present application provides a device for tracing the source of biochemical oxygen-consuming organic matter in a water environment, which can implement the above-mentioned method embodiment. Its implementation principle and technical effect are similar, and this embodiment will not be repeated here.

[0143] Figure 5 This is a hardware structure diagram for tracing the source of biochemical oxygen-consuming organic matter in water environments provided in the embodiments of the present application. Figure 5 As shown, the traceability detection device 500 for biochemical oxygen-consuming organic matter in the water environment includes:

[0144] at least one processor 501 and

[0145] A memory 502 in communication with at least one processor 501;

[0146] Memory 502 stores computer executable instructions;

[0147] The processor 501 executes the computer-executable instructions stored in the memory 502 , so that the device for tracing the source of biochemical oxygen-consuming organic matter in a water environment executes the method for tracing the source of biochemical oxygen-consuming organic matter in a water environment as described above.

[0148] It should be understood that the processor 501 can be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), etc. A general-purpose processor can be a microprocessor or any conventional processor. The steps of the method disclosed in the invention can be directly implemented by a hardware processor or by a combination of hardware and software modules in the processor.

[0149] The memory 502 may include a high-speed random access memory (RAM), and may also include a non-volatile memory (NVM), such as at least one disk memory, and may also be a USB flash drive, a mobile hard disk, a read-only memory, a disk or an optical disk, etc.

[0150] An embodiment of the present application correspondingly provides a computer-readable storage medium, in which computer-executable instructions are stored. When the computer-executable instructions are executed by a processor, they are used to implement the method for tracing the source of biochemical oxygen-consuming organic matter in a water environment as described above.

[0151] The embodiment of the present application also provides a computer program product. When the computer program is executed by a processor, it is used to implement the above-mentioned method for tracing the source of biochemical oxygen-consuming organic matter in a water environment.

[0152] It should be noted that, for the aforementioned method embodiments, for the sake of simplicity, they are all expressed as a series of action combinations, but those skilled in the art should be aware that the present application is not limited by the described order of actions, because according to the present application, certain steps can be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all optional embodiments, and the actions and modules involved are not necessarily required by the present application.

[0153] It should be further noted that, although the various steps in the flowchart are displayed in sequence according to the indication of the arrows, these steps are not necessarily executed in sequence in the order indicated by the arrows. Unless there is a clear description in this article, the execution of these steps is not strictly limited in order, and these steps can be executed in other orders. Moreover, at least a portion of the steps in the flowchart may include multiple sub-steps or multiple stages, and these sub-steps or stages are not necessarily executed at the same time, but can be executed at different times, and the execution order of these sub-steps or stages is not necessarily to be carried out in sequence, but can be executed in turn or alternately with other steps or at least a portion of the sub-steps or stages of other steps.

[0154] It should be understood that the above-mentioned device embodiments are only illustrative, and the device of the present application can also be implemented in other ways. For example, the division of units / modules in the above-mentioned embodiments is only a logical function division, and there may be other division methods in actual implementation. For example, multiple units, modules or components can be combined, or can be integrated into another system, or some features can be ignored or not executed.

[0155] In addition, unless otherwise specified, each functional unit / module in each embodiment of the present application may be integrated into one unit / module, each unit / module may exist physically separately, or two or more units / modules may be integrated together. The above-mentioned integrated unit / module may be implemented in the form of hardware or in the form of a software program module.

[0156] If the integrated unit / module is implemented in the form of hardware, the hardware may be a digital circuit, an analog circuit, etc. The physical implementation of the hardware structure includes but is not limited to transistors, memristors, etc. Unless otherwise specified, the processor may be any appropriate hardware processor, such as a CPU, a GPU, an FPGA, a DSP, an ASIC, etc. Unless otherwise specified, the storage unit may be any appropriate magnetic storage medium or magneto-optical storage medium, such as a resistive random access memory (RRAM), a dynamic random access memory (DRAM), a static random access memory (SRAM), an enhanced dynamic random access memory (EDRAM), a high-bandwidth memory (HBM), a hybrid memory cube (HMC), etc.

[0157] If the integrated unit / module is implemented in the form of a software program module and sold or used as an independent product, it can be stored in a computer-readable memory. Based on this understanding, the technical solution of the present application, or the part that contributes to the prior art, or all or part of the technical solution can be embodied in the form of a software product. The computer software product is stored in a memory, including a number of instructions to enable a computer device (which can be a personal computer, server or network device, etc.) to execute all or part of the steps of the various embodiments of the present application. The aforementioned memory includes: U disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), mobile hard disk, disk or optical disk and other media that can store program codes.

[0158] In the above embodiments, the description of each embodiment has its own emphasis. For the part not described in detail in a certain embodiment, please refer to the relevant description of other embodiments. The technical features of the above embodiments can be combined arbitrarily. In order to make the description concise, all possible combinations of the technical features in the above embodiments are not described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0159] Those skilled in the art will readily appreciate other embodiments of the present application after considering the specification and practicing the invention disclosed herein. The present application is intended to cover any modification, use or adaptation of the present application, which follows the general principles of the present application and includes common knowledge or customary techniques in the art that are not disclosed in the present application. The specification and examples are intended to be exemplary only, and the true scope and spirit of the present application are indicated by the following claims.

[0160] It should be understood that the present application is not limited to the precise structures that have been described above and shown in the drawings, and that various modifications and changes may be made without departing from the scope thereof. The scope of the present application is limited only by the appended claims.

Claims

1. A method for tracing the source of biochemical oxygen-consuming organic matter in a water environment, characterized in that: Applicable to computer equipment, including: Collect water samples from the environment to be tested; Obtaining a first carbon stable isotope composition characteristic and a first substance concentration of the water sample under a first condition, and a second carbon stable isotope composition characteristic and a second substance concentration of the water sample under a second condition, wherein the first condition is before an aerobic degradation culture experiment of the environmental water sample, and the second condition is after an aerobic degradation culture experiment of the environmental water sample; Using the molar mass balance equation of carbon element, inverse calculation is performed on the first carbon stable isotope composition characteristic, the first substance molar concentration, the second carbon stable isotope composition characteristic and the second substance molar concentration to obtain a calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected; According to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, a comparison process is performed in a pre-established carbon stable isotope composition characteristic fingerprint database to obtain corresponding source data, wherein the source data is the source data of the biochemical oxygen-consuming organic matter in the environment to be detected.

2. The method according to claim 1, characterized in that The calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected is compared with a pre-established carbon stable isotope composition characteristic fingerprint database to obtain corresponding source data, including: According to the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, a comparison process is performed in a pre-established carbon stable isotope composition characteristic fingerprint database to determine at least one target source type of the biochemical oxygen-consuming organic matter in the environment to be detected; According to the at least one target source type, the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be detected is determined.

3. The method according to claim 2, characterized in that The step of determining the composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be detected according to the at least one target source type includes: If there are multiple target source types, then obtaining the average value and standard deviation of the carbon stable isotope composition characteristics of the target source type; The composition ratio of each target source type corresponding to the biochemical oxygen-consuming organic matter in the environment to be tested is calculated by using the calculated value of the carbon stable isotope composition characteristic, the average value and standard deviation of the carbon stable isotope composition characteristic of the target source type as prior information and the chemical mass balance formula as a constraint, using the Markov chain Monte Carlo algorithm.

4. The method according to any one of claims 1 to 3, characterized in that: Before collecting the water sample of the environment to be tested, the method further includes: Obtain historical environmental data; A carbon stable isotope composition characteristic fingerprint database is established based on historical environmental data corresponding to preset source types, wherein the source types include at least one of domestic sewage, organic industrial wastewater, C3 plant debris, C4 plant debris, phytoplankton residues, and soil.

5. The method according to any one of claims 1 to 3, characterized in that: The step of obtaining a first carbon stable isotope composition characteristic and a first substance concentration of the water sample under a first condition comprises: extracting the dissolved inorganic carbon of the water sample under the first condition by chemical precipitation; A first carbon stable isotope composition characteristic and a first molar concentration of the dissolved inorganic carbon under the first condition are obtained.

6. The method according to any one of claims 1 to 3, characterized in that: The molar mass balance equation is: in, is the calculated value of the carbon stable isotope composition characteristic of the biochemical oxygen-consuming organic matter in the environment to be detected, is the second carbon stable isotope composition characteristic, is the concentration of the second substance, It is the first carbon stable isotope composition characteristic. is the concentration of the first substance.

7. The method according to any one of claims 1 to 3, characterized in that: After the calculated carbon stable isotope composition characteristic value of the biochemical oxygen-consuming organic matter in the environment to be detected is compared with a pre-established carbon stable isotope composition characteristic fingerprint database to obtain corresponding source data, the method further includes: The source data of the biochemical oxygen-consuming organic matter in the environment to be detected is sent to a user terminal, so that the source data of the biochemical oxygen-consuming organic matter in the environment to be detected is displayed on the user terminal.

8. A traceability detection device for biochemical oxygen-consuming organic matter in aquatic environment, characterized in that: include: at least one processor; as well as a memory communicatively connected to the at least one processor; wherein, The memory stores instructions executable by the at least one processor, and the instructions are executed by the at least one processor so that the at least one processor can execute the method for tracing the source of biochemical oxygen-consuming organic matter in a water environment as described in any one of claims 1 to 7.

9. A computer-readable storage medium, characterized in that: The computer-readable storage medium stores computer-executable instructions, which, when executed by a processor, are used to implement the method for tracing the source of biochemical oxygen-consuming organic matter in a water environment according to any one of claims 1 to 7.

10. A computer program product, comprising a computer program, characterized in that When the computer program is executed by a processor, the method for tracing the source of biochemical oxygen-consuming organic matter in a water environment as described in any one of claims 1 to 7 is implemented.

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