Device and method for testing selective predation pests of neoseiulus barkeri
By designing a test device for Pasteuris's new squid mites, studying its predation preference for pests such as Western flower thrips, the problem of how to improve the efficiency of biological control is solved, and the effect of scientific planning of the delivery volume and systematic research on predation behavior is achieved.
Patent Information
- Application Number
- CN202411855037.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-17
- Publication Date
- 2025-02-28
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Figure CN119522878A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of biological control. Background Art
[0002] Neoseiul us barkeri is a predatory mite widely used in the field of biological control. It can effectively control a variety of harmful mites and small sap-sucking pests. Neoseiul us barkeri is tiny, with a general body width of about 0.1 mm. As a biological control agent, it has a wide range of application potentials. It can effectively control a variety of agricultural pests and mites, reduce the use of chemical pesticides, is environmentally friendly, and helps to achieve green control. Neoseiul us barkeri has been used to control a variety of harmful mites, such as truncate spider mites and two-spotted spider mites, as well as small sap-sucking pests, such as western flower thrips. During the tobacco field period, the number of western flower thrips can be effectively controlled by releasing Neoseiul us barkeri on tobacco and surrounding weeds. The more times it is released, the better the control effect on western flower thrips. The body width of the nymph of western flower thrips is generally not less than 0.3mm. The insect stage includes five stages: egg, nymph, prepupa, pupa and adult. The egg is 0.2mm long and the adult is approximately between 1 and 1.7mm.
[0003] However, Neoseiulus barkeri has different predation preferences for different developmental stages of different pests. In order to improve the efficiency of biological control and scientifically plan the release amount of Neoseiulus barkeri, experiments were conducted on how to scientifically study the predation preference behavior of Neoseiulus barkeri for pests such as western flower thrips. Summary of the invention
[0004] The purpose of the present invention is: in order to solve the above technical problems, the first purpose of the present invention is to provide a testing device for the selective predation of pests by Neoseiulus barkeri, which is convenient for setting various test adjustments and testing the predation preference of Neoseiulus barkeri on pests such as western flower thrips.
[0005] In order to achieve the above-mentioned purpose, the present invention specifically adopts the following technical solutions:
[0006] A testing device for selective predation of pests by Neoseiulus barkeri comprises several test groups, each test group comprises a base, a bracket, and a delivery part, the bracket is arranged on the base, the delivery part is rotatably connected to the bracket, the bracket is provided with a driving component for driving the delivery part to rotate, no less than three prey parts are evenly distributed around the delivery part, the prey parts are used for delivering pests, a channel is provided between the prey parts and the delivery part, a blocking net is provided between the prey parts and the channel, the mesh size of the blocking net exceeds 0.1 mm and is smaller than the diameter of the pests to be delivered, an anti-escape gauze is provided at the upper opening of the delivery part, the mesh size of the anti-escape gauze is less than 0.1 mm, the upper opening of the prey part is also provided with an anti-escape gauze, and a marking component for marking feeding activities is also provided.
[0007] Through the above scheme, multiple test groups are set up to facilitate simultaneous repeated tests. By setting up multiple hunting parts and connecting the delivery parts through channels, pests of different insect stages are conveniently put into the hunting parts, and a blocking net is set up to prevent pests from entering the channel without affecting the entry of the seiyuen mites into the hunting parts for feeding. At the same time, an anti-escape gauze is set up to prevent the seiyuen mites from escaping from the hunting parts and the upper openings of the delivery parts. The seiyuen mites are marked in conjunction with the marking component to facilitate the observation of the feeding situation of the seiyuen mites in each hunting part. By setting up different hunting parts, it is convenient to intuitively observe the feeding tendency of the seiyuen mites. At the same time, by setting up anti-escape gauze and blocking nets in various places, pests and seiyuen mites of various states are restricted to activities in designated areas. The hunting parts and the delivery parts are driven to rotate slowly by the driving component to reduce the test variables in each area of the device. The entire device has a simple structure, diverse functions, and is easy to operate. It can carry out predation tests on pests of different insect stages, such as western flower thrips, and is convenient for systematically studying the predation preference behavior of the new small seiyuen mites on pests such as western flower thrips.
[0008] Furthermore, the base, the delivery portion, and the channel are all made of transparent materials.
[0009] Through the above scheme, the transparent material makes it easy to observe and record the predation behavior and movement trajectory of the mites.
[0010] Furthermore, the marking component includes a fluorescent dye liquid spray gun and a processing tank, and is also provided with a high-speed camera and a fluorescent imaging microscope that are matched with the hunting part.
[0011] Through the above scheme, a fluorescent dye liquid spray gun and a processing tank are set up, the mites are put into the processing tank in advance and dyed by the fluorescent dye liquid spray gun, and then the experiment is carried out. The fluorescent dye marking makes it convenient to mark the mites. With the high-speed camera and fluorescent imaging microscope, it is convenient to mark and continuously observe and record the mites. At the same time, the high-speed camera is convenient for recording the movement trajectory and predation behavior of the mites, and the fluorescent imaging microscope is convenient for further observation of the mites. The observation effect is good due to the tiny size of the mites.
[0012] Furthermore, a test box is provided to match the test group, and a temperature and humidity controller and a light control element are provided in the test box.
[0013] Through the above scheme, the test box is equipped with a temperature and humidity controller and a light control element, which is convenient for forming specific temperature, humidity and light conditions inside the test box, facilitating the study of the feeding habits and activity of Seius mites in different environments, and improving the accuracy and research scope of the test of this device.
[0014] Furthermore, the driving component is any one of a motor drive and a spring drive.
[0015] Through the above scheme, a motor drive or a spring drive is set to realize the rotation of the delivery part, and then the hunting part is linked to rotate synchronously, with a high degree of automation.
[0016] The second object of the present invention is to provide a test method for selective predation of pests by Neoseiulus barkeri, comprising the following steps:
[0017] Step S1: starving Neoseius pasteurii and selecting the number of test groups according to the number of experiments;
[0018] Step S2: Pests of different insect stages to be released are placed into the prey section, food is provided, a blank control group is set, and each prey section is marked;
[0019] Step S3: marking Neoseiulus pasteurii;
[0020] Step S4: putting Neoseiulus pasteurii into the delivery section;
[0021] Step S5: Using a natural environment or a test box according to needs to continuously record the activity track of Neoseius pasteurii;
[0022] Step S6: After a period of time, check the amount of prey on each stage of pests;
[0023] Step S7: Continue steps S1 to S6, repeat at least 20 times, and record relevant data;
[0024] Step S8: Based on the data results of step S7, a comprehensive analysis is performed to draw an analysis chart of the selective predation of pests by Neoseiius barkeri.
[0025] Through the above scheme, multiple test groups are set up to facilitate simultaneous repeated experiments. The New Seius barker mites are starved to stimulate their predatory desire. At the same time, pests of different insect stages are put into different prey parts, and matched with a blank control group to achieve mutual isolation and no mutual influence. Then, according to the needs, a natural environment or a test box is used to simulate the natural environment and conduct experiments under fixed temperature, humidity and light to study the predation of mites under different conditions. Then, the prey amount of pests is checked, the results are recorded, and repeated experiments are used to reduce accidental effects and eliminate unreasonable factors. According to the results, an analysis chart of the selective predation of New Seius barker mites on pests is prepared. The whole process is reasonably planned. According to the habits and physical data of New Seius barker mites, scientific restriction measures are set to facilitate the control of different experimental conditions and variables. The test results are accurate, the functions are diverse, and the operation is convenient. Predation tests can be carried out on pests of different insect stages, such as western flower thrips, which is convenient for systematic research on the predation preference behavior of New Seius barker mites on pests such as western flower thrips.
[0026] Furthermore, in step S1, the Neoseiius barkeri mites can be female adult mites, male adult mites, larvae of different ages, or a mixture to simulate the predation orientation of Neoseiius barkeri mites in different insect stages, and the number of Neoseiius barkeri mites released can be adjusted according to demand.
[0027] Furthermore, in step S5, a test chamber test process is used to control different temperature, humidity and light conditions to simulate the predation orientation of Neoseiius pasteurii under different conditions.
[0028] In step S2, the pests to be released include small sap-sucking pests, namely, Bemisia tabaci or Western flower thrips.
[0029] The beneficial effects of the present invention are as follows:
[0030] 1. The present invention has a simple structure. It is convenient to put pests of different insect stages into the hunting parts by setting multiple hunting parts and connecting the delivery parts through channels. A blocking net is set to prevent pests from entering the channels without affecting the entry of the mites into the hunting parts for feeding. At the same time, an anti-escape gauze is set to prevent the mites from escaping from the hunting parts and the upper openings of the delivery parts. The marking component is used to mark the mites, which is convenient for observing the feeding situation of the mites in each hunting part. By setting different hunting parts, it is convenient to intuitively observe the feeding tendency of the mites. At the same time, by setting the anti-escape gauze and blocking nets in various places, pests and mites of various states are restricted to move in designated areas. The hunting parts and the delivery parts are driven to rotate slowly by the driving component to reduce the test variables in each area of the device. The whole device has a simple structure, diverse functions, and is easy to operate. It can carry out predation tests on pests of different insect stages, such as western flower thrips, and is convenient for systematically studying the predation preference behavior of the new small mites of barker on pests such as western flower thrips.
[0031] 2. The test box is equipped with a temperature and humidity controller and a light control element to facilitate the formation of specific temperature, humidity and light conditions inside the test box, so as to facilitate the study of the feeding habits and activity of Seiurus mites in different environments and improve the accuracy and research scope of the test of this device.
[0032] 3. The whole process is planned reasonably. According to the habits and physical data of Neoseiulus barkeri, the restriction measures are scientifically set to facilitate the control of different experimental conditions and variables. The test results are accurate, the functions are diverse, and the operation is convenient. It can be used to conduct predation tests on pests of different insect stages, such as western flower thrips, so as to facilitate the systematic study of the predation preference behavior of Neoseiulus barkeri on pests such as western flower thrips. BRIEF DESCRIPTION OF THE DRAWINGS
[0033] Figure 1 It is a schematic diagram of the structure of the test group part of the present invention;
[0034] Figure 2 It is a schematic diagram of the cross-sectional structure of the hunting part of the present invention;
[0035] Figure 3 It is a partial structural schematic diagram of the test box and marking components of the present invention.
[0036] Attached figures: 11. base; 12. bracket; 13. launching part; 14. hunting part; 15. passage; 16. blocking net; 17. anti-escape gauze; 18. fluorescent dye liquid spray gun; 19. processing tank; 20. test box; 21. temperature and humidity controller; 22. light control element. DETAILED DESCRIPTION
[0037] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.
[0038] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0039] Example 1
[0040] like Figure 1 and Figure 2 and Figure 3As shown, the present embodiment provides a test device for selective predation of pests by Neoseiulus barkeri, including several test groups, each test group including a base 11, a bracket 12, and a delivery portion 13, the bracket 12 is arranged on the base 11, the base 11 is used to fix the bracket 12, the delivery portion 13 is rotatably connected with the bracket 12, a driving component for driving the delivery portion 13 to rotate is arranged on the bracket 12, the driving component is any one of a motor drive and a spring drive, and a servo motor drive is adopted in the present embodiment, and no less than three prey portions 14 are evenly distributed around the delivery portion 13, and four prey portions 14 are arranged in the present embodiment, and the prey portions 14 are used to deliver pests In this embodiment, western flower thrips is selected as the prey test object, a passage 15 is provided between the hunting part 14 and the delivery part 13, and a blocking net 16 is provided between the hunting part 14 and the passage 15. The mesh size of the blocking net 16 exceeds 0.1 mm and is smaller than the diameter of the pest to be delivered. In this embodiment, the mesh size of the blocking net 16 exceeds 0.1 mm and is smaller than 0.3 mm, which is smaller than the body width of the nymph of western flower thrips. The upper opening of the delivery part 13 is provided with an anti-escape gauze 17, and the mesh size of the anti-escape gauze 17 is less than 0.1 mm. The upper opening of the hunting part 14 is also provided with an anti-escape gauze 17, and a marking component for marking feeding activities is also provided.
[0041] Therefore, multiple test groups are set up to facilitate simultaneous repeated tests. Multiple feeding parts 14 are set up, and the delivery part 13 is connected through a channel 15, so that pests of different insect stages can be put into the feeding part 14. A blocking net 16 is set up to prevent pests from entering the channel 15, which does not affect the entry of the mites into the feeding part 14 to feed. At the same time, an anti-escape gauze 17 is set up to prevent the mites from escaping from the feeding part 14 and the upper opening of the delivery part 13, which does not affect the ventilation of the device. The marking component is used to mark the mites, which is convenient for observing the feeding situation of the mites in each feeding part 14. By setting different hunting parts 14, it is convenient and intuitive to observe the feeding tendency of Seius mites. At the same time, by setting anti-escape gauze nets 17 and blocking nets 16 in various places, pests and Seius mites in various states are restricted to move in designated areas. The hunting part 14 and the delivery part 13 are driven to rotate slowly by the driving component to reduce the test variables in each area of the device. The whole device has a simple structure, diverse functions, and is easy to operate. It can be used to carry out predation tests on pests of different insect stages, such as western flower thrips, which is convenient for systematically studying the predation preference behavior of New Seius mites on pests such as western flower thrips.
[0042] Reference Figure 1 and Figure 2 and Figure 3In order to improve the test effect and facilitate operation, the base 11, the delivery part 13, and the channel 15 are all made of transparent materials. The marking components include a fluorescent dye liquid spray gun 18 and a processing tank 19, and a high-speed camera and a fluorescent imaging microscope (high-speed camera and fluorescent imaging microscope are common equipment, not shown in the figure) are provided in conjunction with the predator part 14. The transparent material is convenient for observing and recording the predation behavior and movement trajectory of the mites. The fluorescent dye liquid spray gun 18 and the processing tank 19 are provided. The mites are placed in the processing tank 19 in advance and dyed by the fluorescent dye liquid spray gun 18, and then the test is carried out. The fluorescent dye marking is convenient for marking the mites. The high-speed camera and the fluorescent imaging microscope are convenient for marking and continuously observing and recording the mites. At the same time, the high-speed camera is convenient for recording the movement trajectory and predation behavior of the mites. The fluorescent imaging microscope is convenient for further observation of the mites. The observation effect is good for the tiny size of the mites.
[0043] In order to further increase the experimental stability and facilitate control, refer to Figure 1 and Figure 3 In addition, a test box 20 is provided to match the test group. A temperature and humidity controller 21 and a light control element 22 are provided in the test box 20. A power supply adapted to the servo motor is also provided in the test box 20. The light control element 22 can be a common fill light with adjustable light intensity. The test box 20 is equipped with the temperature and humidity controller 21 and the light control element 22, so as to form specific temperature, humidity and light conditions inside the test box 20, so as to study the feeding habits and activity of Seius mites in different environments, and improve the accuracy and research scope of the test of the device.
[0044] Reference Figure 1 and Figure 2 and Figure 3 , a test method for selective predation of pests by Neoseiulus barkeri, comprising the following steps:
[0045] Step S1: starving Neoseius barkeri for 24 hours. In this embodiment, adult female Neoseius barkeri mites are used. The number of test groups is selected according to the number of experiments. Generally, multiple experimental groups are started simultaneously to facilitate the simultaneous start of multiple groups of experiments.
[0046] Step S2: Pests of different insect stages are put into the feeding section 14 and provided with food. In this embodiment, 10 first-instar nymphs, 10 second-instar nymphs, and 10 adults are put into three feeding sections 14, a blank control group is set, and each feeding section 14 is marked;
[0047] Step S3: marking Neoseius pasteurii mites, placing the mites into a treatment tank 19 in advance and dyeing them with a fluorescent dye spray gun 18, and then conducting a test;
[0048] Step S4: putting a marked Neoseiius pasteurii mite into the delivery section 13;
[0049] Step S5: using a natural environment or a test box 20 according to the needs. In this embodiment, a natural environment is used to conduct the test, and the activity track of Neoseiulus pasteurii is continuously recorded;
[0050] Step S6: After a period of time, in this embodiment, the amount of prey on each stage of pests is checked after 12 hours;
[0051] Step S7: Continue steps S1 to S6, repeat at least 20 times, and record relevant data;
[0052] Step S8: Based on the data results of step S7, a comprehensive analysis is performed to draw an analysis chart of the selective predation of pests by Neoseiius barkeri.
[0053] Through the above scheme, multiple test groups are set up to facilitate simultaneous repeated experiments. The New Seius barker mites are starved to stimulate their predatory desire. At the same time, pests of different insect stages are put into different prey sections 14, and matched with a blank control group to achieve mutual isolation and no mutual influence. Then, a natural environment is adopted or a test box 20 is used according to needs to simulate the natural environment and conduct experiments under fixed temperature, humidity and light conditions to study the predation of mites under different conditions. Then, the prey amount of pests is checked, the results are recorded, and repeated experiments are used to reduce accidental effects and eliminate unreasonable factors. According to the results, an analysis chart of the selective predation of New Seius barker mites on pests is prepared. The whole process is reasonably planned. According to the habits and physical data of New Seius barker mites, scientific restriction measures are set to facilitate the control of different experimental conditions and variables. The test results are accurate, the functions are diverse, and the operation is convenient. Predation tests can be carried out on pests of different insect stages, such as western flower thrips, to facilitate systematic research on the predation preference behavior of New Seius barker mites on pests such as western flower thrips.
[0054] Furthermore, in step S1, the Neoseiius barkeri mites can be female adult mites, male adult mites, larvae of different ages, or a mixture to simulate the predation orientation of Neoseiius barkeri mites in different insect stages. Meanwhile, the number of Neoseiius barkeri mites released is adjusted according to demand. In this embodiment, one female adult mites is released.
[0055] Furthermore, in step S5, the test box 20 is used to control different temperature, humidity and light conditions during the test process to simulate the predation orientation of Neoseiulus pasteurii under different conditions. In this embodiment, the test is conducted in a natural environment.
[0056] In step S2, the pests to be released include small sap-sucking pests, namely, Bemisia tabaci or Western flower thrips.
[0057] As follows, we selected farmland and vegetable fields as the natural environment to set up the test group, and selected western flower thrips as the prey.
[0058] Experiment 1: Analysis of the selective predation of Neoseiulus barkeri on western flower thrips in natural environment under farmland environment.
[0059] Western Flower Thrips Neoseiulus pasteurii Western Flower Thrips Number Number of prey Number of visits to the region 1st instar nymph 1 female adult Microseiidae 10 0 2 2nd instar nymph 1 female adult Microseiidae 10 2 3 adult 1 female adult Microseiidae 10 6 14 Blank Group 1 female adult Microseiidae Blank Group 1
[0060] Experiment 2: Analysis of the selective predation of Neoseiius barker on western flower thrips in natural environment under vegetable field environment.
[0061] Western Flower Thrips Number of Neoseiulus pasteurii Western Flower Thrips Number Number of prey Number of visits to the region 1st instar nymph 1 female adult Microseiidae 10 0 2 2nd instar nymph 1 female adult Microseiidae 10 3 3 adult 1 female adult Microseiidae 10 4 14 Blank Group 1 female adult Microseiidae Blank Group 2
[0062] The above experimental data in various natural environments can draw the following preliminary conclusions: The predation desire of Neoseiulus barkeri on adult western flower thrips is higher than that on nymphs, and the predation ability in vegetable fields is slightly lower than that in farmlands. The preliminary reason for this may be that adult western flower thrips usually have stronger mobility and the ability to find food. They may be more active in finding food sources, so they are more easily detected by mites, triggering predation by mites. The nymph stage is usually less active, and they rely more on plant sap for food rather than actively looking for food, so they encounter mites less. At the same time, since the content of pesticides and plant odor chemicals in vegetable fields is higher than that in farmlands, this may affect the attraction of different stages of western flower thrips to Neoseiulus barkeri, and may also affect the vitality of mites.
[0063] Embodiment 2
[0064] The structure of the second embodiment is basically the same as that of the first embodiment, except that, in the second embodiment, a test box 20 is used to conduct the test, and the test is conducted by adjusting the temperature and light conditions.
[0065] As follows, we conducted the experiment in test box 20 and set up the test group, selected a female adult mite of Microseiidae as the predator, and selected Western Flower Thrips as the prey.
[0066] Experiment 3: Selective predation of Neospermum barbatum on western flower thrips under different light temperatures
[0067]
[0068] The experimental data of the above test groups under 20℃ in each test box can draw the following preliminary conclusions: The selective predation of Neoseiulus barkeri on western flower thrips is significantly affected by temperature and light conditions. In the temperature range of 15-28℃, the attack coefficient, predation ability and maximum daily predation of Neoseiulus barkeri on western flower thrips all showed an upward trend. The amount of predation increased with the increase of temperature, while the treatment time was shortened. When the temperature reached 35℃, the predation amount decreased.
[0069] A plurality of test groups are set up to facilitate simultaneous repeated experiments, wherein the New Seius barker mites are starved to stimulate their predatory desire, and pests of different insect stages are put into different prey sections 14, and matched with a blank control group, to achieve isolation and no mutual influence, and then a natural environment is adopted or a test box 20 is used as required to simulate the natural environment and to conduct experiments under fixed temperature, humidity and light conditions to study the predation of mites under different conditions, and then the predation amount of pests is checked, the results are recorded, and repeated experiments are adopted to reduce accidental effects and exclude unreasonable factors, and an analysis chart of the selective predation of New Seius barker mites on pests is prepared according to the results, the whole process is reasonably planned, and the restriction means are scientifically set according to the habits and physical data of New Seius barker mites to facilitate the control of different experimental conditions and variables, the test results are accurate, the functions are diverse, and the operation is convenient, and predation tests can be carried out on pests of different insect stages, such as western flower thrips, to facilitate the systematic study of the predation preference behavior of New Seius barker mites on pests such as western flower thrips.
Claims
1. A testing device for selective predation of pests by Neoseiulus barkeri, characterized in that: The invention comprises a plurality of test groups, each test group comprises a base (11), a bracket (12), and a delivery portion (13), wherein the bracket (12) is arranged on the base (11), the delivery portion (13) is rotatably connected to the bracket (12), the bracket (12) is provided with a driving component for driving the delivery portion (13) to rotate, and no less than three prey portions (14) are evenly distributed around the delivery portion (13), the prey portions (14) are used for delivering pests, and the prey portions (14) are connected to the The delivery parts (13) are connected by a passage (15), a blocking net (16) is provided between the hunting part (14) and the passage (15), the blocking net (16) has a mesh size greater than 0.1 mm and smaller than the diameter of the pests to be delivered, an anti-escape gauze (17) is provided at the upper opening of the delivery part (13), the mesh size of the anti-escape gauze (17) is less than 0.1 mm, an anti-escape gauze (17) is also provided at the upper opening of the hunting part (14), and a marking component for marking feeding activities is also provided.
2. A testing device for selective predation of pests by Neoseiulus barkeri according to claim 1, characterized in that: The base (11), the delivery portion (13), and the channel (15) are all made of transparent materials.
3. A testing device for selective predation of pests by Neoseiulus barkeri according to claim 1, characterized in that: The marking component comprises a fluorescent dye liquid spray gun (18) and a processing tank (19), and is also provided with a high-speed camera and a fluorescent imaging microscope matched with the hunting part (14).
4. A testing device for selective predation of pests by Neoseiulus barkeri according to claim 1, characterized in that: A test box (20) is also provided to match the test group, and a temperature and humidity controller (21) and a light control element (22) are provided in the test box (20).
5. A testing device for selective predation of pests by Neoseiulus barkeri according to claim 1, characterized in that: The driving component is any one of a motor drive and a spring drive.
6. A test method for selective predation of pests by Neoseiulus barkeri according to claim 1, characterized in that: The following steps are involved: Step S1: starving Neoseius pasteurii and selecting the number of test groups according to the number of experiments; Step S2: Pests of different insect stages to be released are placed into the feeding part (14), food is provided, a blank control group is set up, and each feeding part (14) is marked; Step S3: marking Neoseiulus pasteurii; Step S4: putting Neoseiulus pasteurii into the placing part (13); Step S5: using a natural environment or a test box (20) according to needs to continuously record the activity track of Neoseiius pasteurii; Step S6: After a period of time, check the amount of prey on each stage of pests; Step S7: Continue steps S1 to S6, repeat at least 20 times, and record relevant data; Step S8: Based on the data results of step S7, a comprehensive analysis is performed to draw an analysis chart of the selective predation of pests by Neoseiius barkeri.
7. A test method for selective predation of pests by Neoseiulus barkeri according to claim 6, characterized in that: In step S1, the Neoseiius barkeri mites can be female adult mites, male adult mites, larvae of different ages, or a mixture to simulate the predation orientation of Neoseiius barkeri mites in different insect stages. Meanwhile, the number of Neoseiius barkeri mites released is adjusted according to demand.
8. A test method for selective predation of pests by Neoseiulus barkeri according to claim 6, characterized in that: In step S5, the test box (20) is used to control different temperature, humidity and light conditions during the test process to simulate the predation orientation of Neoseiulus pasteurii under different conditions.
9. A test method for selective predation of pests by Neoseiulus barkeri according to claim 6, characterized in that: In step S2, the pests to be released include small sap-sucking pests, namely, Bemisia tabaci or Western flower thrips.
Citation Information
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