Mosquito repelling composition and mosquito repelling liquid

Abstract

The invention discloses a mosquito repelling composition and mosquito repelling liquid. The mosquito-repelling composition comprises citronellal, geraniol, citronellol, limonene, elemol, isolongifolene, alpha-pilanol, cedrol, alpha-cedrene, beta-maselemene, benzyl alcohol, caryophyllene and the like, the mosquito-repelling composition is good in mosquito-repelling effect, harmonious in fragrance and small in irritation to human sensory organs, the mosquito-repelling composition serves as an active component to be used for mosquito-repelling liquid, the mosquito-repelling effect is good, and the mosquito-repelling effect is good. The smell is easy to accept by a user, and the mosquito repellent is convenient to use and can be used as spray liquid or electric heating volatile mosquito repellent liquid.

Description

Technical Field

[0001] The invention relates to a mosquito repellent composition and a mosquito repellent liquid, in particular to a plant mosquito repellent composition of pure natural plant active ingredients, and also to a mosquito repellent liquid comprising the mosquito repellent composition, belonging to the technical field of mosquito repellent liquid preparation. Background Art

[0002] Mosquitoes belong to the family Culicidae of the order Diptera of the class Insecta, and there are about 3,000 species worldwide. Blood-sucking female mosquitoes are intermediate hosts for other pathogens such as dengue fever, malaria, yellow fever, filariasis, and Japanese encephalitis. The main harm of mosquitoes is the spread of diseases. According to research, mosquitoes spread more than 80 kinds of diseases. Every year, about 700 million people are infected with various diseases by mosquitoes, and one in every seventeen people dies from various diseases transmitted by mosquitoes. Especially in tropical areas, many infectious diseases are often transmitted to humans by mosquitoes. With the arrival of summer every year, mosquitoes also come with it. Mosquito bites not only make people itchy and red, but the more serious problem is the different germs carried by mosquitoes, so mosquito control is of great significance.

[0003] At present, the main means of mosquito control is to use insecticides and repellents. The mosquito control insecticides on the market are mainly chemical synthetic insecticides. Using chemical insecticides to kill mosquitoes is an important measure to control mosquito-borne diseases. In decades of chemical control, due to the extensive use and abuse of chemical insecticides, mosquitoes have developed resistance to a variety of chemical insecticides, and the degree of resistance has also shown a trend of gradually increasing. With the increase in the degree of mosquito resistance, the use of chemical insecticides has to be increased, which not only increases the cost of using chemical insecticides but also seriously pollutes the environment, making the application of chemical insecticides subject to. Mosquito repellents do not directly act on mosquitoes to kill them, but through their chemical effects (such as color, smell, etc.) to make mosquitoes avoid them, thereby achieving the effect of preventing mosquito bites. At present, there are natural mosquito repellents and synthetic chemical mosquito repellents on the market, and chemical repellents account for the majority of the market share, but the toxicity and safety of chemical repellents have always been the focus of everyone's attention. For example, DEET, which is commonly used at present, can inhibit the activity of acetylcholinesterase in the central nervous system of mammals. Long-term or large-scale use can induce nervous system symptoms, and there are many problems such as corrosiveness to plastics and leather, and the smell can easily cause discomfort to people. In addition, chemical repellents cause mosquito resistance to rise rapidly during use, and the decomposition time in the natural environment is long, causing serious residual problems. Natural mosquito repellents with natural plant active ingredients are easy to degrade in the natural environment, not easy to remain, and have little toxicity accumulation problems. They have the advantages of low toxicity and green environmental protection, so that people have turned their attention to plant-derived insecticides and repellents. Natural mosquito repellents with the advantages of fresh smell, low toxicity or non-toxicity, and no burning sensation on the skin have begun to be favored by people. However, the natural mosquito repellents on the market in the prior art have a general mosquito repellent effect, and the odor is irritating, and the fragrance is not harmonious, which is difficult for users to accept. Summary of the invention

[0004] In view of the technical problems existing in the natural mosquito repellents in the prior art, the first object of the present invention is to provide a mosquito repellent composition with good mosquito repellent effect, harmonious fragrance and little irritation to human senses.

[0005] The second object of the present invention is to provide a mosquito repellent liquid, which contains a mosquito repellent composition, has a good mosquito repellent effect, has a smell that is easily accepted by users, and is easy to use. The mosquito repellent liquid can be used as a spray liquid or as an electrothermal volatile mosquito repellent liquid.

[0006] In order to achieve the above technical objectives, the present invention provides a mosquito repellent composition, which comprises the following components in parts by weight: 8 to 17 parts of citronellal; 6 to 15 parts of geraniol; 4 to 10 parts of citronellol; 1.8 to 4 parts of limonene; 1.5 to 3 parts of elemenol; 7 to 15 parts of isolongifolene; 2.5 to 5 parts of α-cedrene; 2 to 5 parts of cedrol; 1.2 to 2.5 parts of α-cedrene; 1.2 to 2.5 parts of β-malolene; 1.2 to 2.4 parts of benzyl alcohol; and 1 to 2.4 parts of caryophyllene.

[0007] As a preferred embodiment, the mosquito repellent composition is composed of the following components in parts by weight: 10-15 parts of citronellal; 8-12 parts of geraniol; 6-8 parts of citronellol; 2-3 parts of limonene; 2-2.5 parts of elemenol; 8-12 parts of isolongifolene; 3-4 parts of α-cedrene; 3-4 parts of cedrol; 1.5-2 parts of α-cedrene; 1.5-2 parts of β-malolene; 1.5-2 parts of benzyl alcohol; and 1.5-2 parts of caryophyllene.

[0008] The components and raw materials in the mosquito repellent composition of the present invention are all derived from natural plant ingredients. These natural plant ingredients are basically used as spices and flavor ingredients in the prior art. For example, citronellal has the aroma of lemon, lily and rose, citronellol has the sweet rose aroma, geraniol has a mild and sweet rose scent with a bitter taste, limonene has a lemon-like aroma, elemiol has a spicy and creamy aroma with a citrus peel top aroma, isolongifolene has a woody aroma, α-pyrocatechol has a sweet geranium oil-rose aroma, honey sweetness, and a slightly light resin aroma, and the base aroma has a herbal aroma, cedarwood has a mild cedarwood aroma, and α-cedarwood has a mild cedarwood aroma. The present invention combines and uses these natural fragrance components in appropriate proportions to produce a special smell by synergistically matching these fragrance components. For humans, this smell is a fragrance that humans like and are easy to accept. This fragrance has good harmony, is non-irritating, and has high sensory quality. However, for mosquitoes, it is a smell that mosquitoes hate and are unwilling to approach, thereby showing a better mosquito repellent effect, which is unexpected.

[0009] The present invention also provides a mosquito repellent liquid, whose active ingredient is the mosquito repellent composition. The mosquito repellent liquid provided by the present invention uses the mosquito repellent composition as an active ingredient, is mixed with a conventional solvent, and can also be selectively mixed with a small amount of auxiliary agents. The mosquito repellent liquid can be used directly as a spray liquid or as an electrothermal volatile mosquito repellent liquid.

[0010] As a preferred solution, the mosquito repellent liquid comprises a mosquito repellent composition, a solvent and an auxiliary agent.

[0011] As a preferred solution, the solvent is water, or an alcohol aqueous solution composed of ethanol and water in a mass percentage of 5-10%:90-95%. More preferably, it is an alcohol aqueous solution. The solubility and dispersibility of the mosquito repellent composition using ethanol and water in an appropriate ratio as solvents is better.

[0012] As a preferred solution, the auxiliary agent includes an antioxidant and / or a pH regulator.

[0013] As a preferred solution, the antioxidant includes at least one of carotenoids, ascorbic acid, ascorbyl palmitate, isoascorbic acid and sodium isoascorbate. These antioxidants are mainly used to eliminate a small amount of oxygen dissolved in the mosquito repellent liquid, to prevent the raw materials in the mosquito repellent composition from being oxidized and ineffective, or even producing odor.

[0014] As a preferred solution, the pH regulator is a phosphate buffer. The preferred pH regulator can ensure that the pH of the mosquito repellent liquid is close to that of human skin, thereby reducing the irritation to human skin during the use of the mosquito repellent liquid.

[0015] As a preferred solution, the mosquito repellent liquid comprises the following components in parts by weight: 0.1 to 0.5 parts of the mosquito repellent composition;

[0016] 300 parts of solvent; 0.005-0.015 parts of antioxidant; pH adjuster measured to adjust the pH of mosquito repellent liquid to 7-7.5.

[0017] Compared with the prior art, the technical solution of the present invention has the following beneficial technical effects:

[0018] The raw material components used in the mosquito repellent composition of the present invention are all natural plant fragrance components, almost no chemical synthetic components, and are non-toxic to the human body. These natural plant fragrance components are easily degraded in the natural environment. Compared with the common chemical synthetic mosquito repellents in the prior art, it is not only green and environmentally friendly, but also can reduce the drug resistance of mosquitoes and has low toxicity.

[0019] The mosquito repellent composition of the present invention not only has a good mosquito repellent effect, but also has a harmonious fragrance, low irritation, good sensory quality, and is easily accepted by users.

[0020] The mosquito repellent composition of the present invention can be prepared into mosquito repellent liquid, which is convenient to use and can be used as a spray liquid or an electrothermal volatile mosquito repellent liquid. It has a good and lasting mosquito repellent effect and its smell is easily accepted by users. DETAILED DESCRIPTION

[0021] The following examples are intended to further illustrate the present invention rather than to limit the scope of the claims.

[0022] The chemical reagents involved in the following examples are all commercial reagents unless otherwise specified.

[0023] Example 1

[0024] This embodiment uses 17 natural plant fragrance active ingredients as basic raw materials, and takes mosquito repellent effect and fragrance harmony as main considerations to screen out the best mosquito repellent composition and corresponding mosquito repellent liquid formula.

[0025] The 17 active ingredients of spices from natural plants are: citronellal, geraniol, citronellol, limonene, elemenool, isolongifolene, α-ceryl alcohol, cedarwood, α-cedrene, β-oleane, benzyl alcohol, caryophyllene, phenylacetaldehyde, α-ionone, diethyl malonate, menthol and coumarin.

[0026] These 17 fragrance active ingredients were used as single effective ingredients and prepared into mosquito repellent liquid according to the following formula: 0.25 parts of fragrance active ingredients; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; and phosphate buffer was used to adjust the pH of the solution system to 7.2.

[0027] Avoidance effect test method:

[0028] Test materials: Standard test insects raised in the laboratory were used. Aedes albopictus (scientific name: Aedes albopictus, English name: Aedes albopictus) is a common mosquito species in Southeast Asia and China. It is a medium-sized black mosquito. Aedes albopictus has a long and thin tubular organ for collecting blood. In particular, female mosquitoes are very active and fierce blood suckers. The following experiments use female adults of Aedes albopictus that have not sucked blood 3 to 5 days after emergence as test insects.

[0029] Environmental conditions: temperature (26±1)℃, relative humidity (65±10)%.

[0030] Instruments and equipment: Mosquito cage: 400mm long, 300mm wide, 300mm high.

[0031] Test method: refer to the national standard "Indoor efficacy test and evaluation of public health insecticides for pesticide registration Part 9: Repellents" (GB / T13917.9-2009).

[0032] Attack power test: 300 test insects are placed in a mosquito cage. The tester exposes 40mm×40mm of skin on the back of his hand, and the rest of the hand is tightly covered. He puts his hand into the mosquito cage and stays there for 2 minutes. He observes closely and finds that the test insects land. Before their mouthparts pierce the skin, they shake their arms to drive them away. This is recorded as one test insect landing. If more than 30 test insects land on the tester and the test insects, their attack power is qualified and this person and the mosquitoes in this cage can be subjected to the repellency test.

[0033] Repellency test: Select 6 testers with qualified attack power (half male and half female, and should not drink alcohol, tea or coffee before and during the test, and should not use products containing fragrances). Draw a 50mm×50mm skin area on the back of each hand, and press 1.5μL / cm on one hand. 2 Apply the tested mosquito repellent evenly with a dosage of 100mg / kg, exposing 40mm×40mm of the skin and tightly covering the rest. Use the other hand as a blank control. Apply the repellent for 2h, put the hand into a mosquito cage with qualified attack power for 2min, observe whether there are mosquitoes coming to land and suck blood, and then test it every 1h. The repellent will become ineffective as long as one mosquito comes to suck blood. Record the effective protection time (h) of the mosquito repellent. Perform a control test on the control hand each time. The test insects with qualified attack power can continue the experiment, while those with unqualified attack power need to be replaced with qualified test insects for the test.

[0034] Calculation: Add up the effective protection time of the drug for 6 subjects and take the average (keep 1 decimal place) as the average protection time.

[0035] Evaluation: The efficacy of the mosquito repellent was evaluated based on the average protection time of the test persons.

[0036]

[0037] Note: The effective protection time is an integer value (rounded off), and the average protection time is the average of the effective protection time of each subject (retain one decimal place).

[0038] It can be seen from Table 1 that when a single fragrance active ingredient is used in mosquito repellent liquid, the average protection time is relatively short, all within 6.5h. At the same time, it can also be seen from Table 1 that when citronellal, geraniol, citronellol, elemiol, α-ceryl alcohol, cedryl, and α-cedryl are used as active ingredients in mosquito repellent liquid alone, they have a certain mosquito repellent effect, and the average protection time exceeds 3h, indicating that these components can be considered as mosquito repellent active ingredients. Therefore, these fragrance active ingredients are combined to investigate their mosquito repellent effect. The seven fragrance active ingredients are mixed in equal mass ratios and prepared into mosquito repellent liquid according to the following formula: 0.25 parts of fragrance active ingredients; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; phosphate buffer solution is used to adjust the pH of the solution system to 7.2. The average protection time of the repellent effect test results of the mosquito repellent liquid formula reaches 11h, indicating that there is a significant synergistic effect between these components, but the fragrance harmony of the composition is poor, the stimulation is large, and the user is not easily accepted, and its fragrance needs to be adjusted. The components used for blending are mainly selected from the fragrance active ingredients with an average protection time of more than 1.5 hours and less than 3 hours. The fragrance active ingredients with an average protection time of less than 1.5 hours are not considered. Several specific optimized mosquito repellent composition formulas are listed as follows (each active ingredient is in equal parts by mass):

[0039] The mosquito repellent composition formula A includes citronellal, geraniol, citronellol, limonene, elemenol, isolongifolene, α-ceryl alcohol, cedrol, α-cedrene, β-oleane, benzyl alcohol and caryophyllene.

[0040] Mosquito repellent composition formula : Citronellal, geraniol, citronellol, limonene, elemenool, isolongifolene, α-ceryl alcohol, cedarwood, α-cedrene, α-ionone and diethyl malonate.

[0041] Mosquito repellent composition formula C: citronellal, geraniol, citronellol, elemenool, α-ceryl alcohol, cedrol, α-cedrene, α-ionone, diethyl malonate and coumarin.

[0042] Mosquito repellent composition formula D: citronellal, geraniol, citronellol, elemenol, α-ceryl alcohol, cedrol, α-cedrene, diethyl malonate, coumarin, isolongifolene and β-oleane.

[0043] The above mosquito repellent composition formulas A~D are prepared into mosquito repellent liquids according to the following formulas: 0.25 parts of the mosquito repellent composition; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; using phosphate buffer to adjust the solution system pH to 7.2; and respectively obtain optimized mosquito repellent liquids A~D.

[0044]

[0045] Note: The effective protection time is an integer value (rounded off), and the average protection time is the average of the effective protection time of each subject (rounded to one decimal place).

[0046] As can be seen from Table 2, the mosquito repellent composition contained in mosquito repellent liquid A, on the basis of selecting citronellal, geraniol, citronellol, elemenol, α-ceryl alcohol, cedryl and α-cedrene as the main active ingredients, is formulated with limonene, isolongifolene, β-oleane, benzyl alcohol and caryophyllene, which further improves the mosquito repellent effect and the harmony of the fragrance is also improved, while the other optimized mosquito repellent liquid formulas B~D have improved harmony in fragrance, but there is no obvious improvement in the mosquito repellent effect. Therefore, the mosquito repellent composition contained in mosquito repellent liquid A is considered as the best formula for further optimization of the composition ratio to obtain the mosquito repellent composition formula with the best effect.

[0047] The dosage ratio of each active component in the mosquito repellent composition formula A was optimized. The optimization principle was mainly to take the active components with an average protection time of more than 3 hours as the main active components, and adjust their dosage ratios in a relatively small range, while the active components with an average protection time of more than 1.5 hours and less than 3 hours were adjusted in a relatively large range. Examples of mosquito repellent composition formulas E to H are as follows:

[0048] Mosquito repellent composition formula E: 10 parts of citronellal; 10 parts of geraniol; 10 parts of citronellol; 5 parts of limonene; 5 parts of elemenol; 10 parts of isolongifolene; 5 parts of α-ceryl alcohol; 5 parts of cedarwood; 5 parts of α-cedrene; 5 parts of β-malolene; 5 parts of benzyl alcohol; 5 parts of caryophyllene;

[0049] Formula F of mosquito repellent composition: 10 parts of citronellal; 10 parts of geraniol; 10 parts of citronellol; 10 parts of limonene; 10 parts of elemenol; 10 parts of isolongifolene; 5 parts of α-ceryl alcohol; 5 parts of cedarwood; 2.5 parts of α-cedrene; 2.5 parts of β-malolene; 2.5 parts of benzyl alcohol; and 2.5 parts of caryophyllene.

[0050] Mosquito repellent composition formula G: 10 parts of citronellal; 10 parts of geraniol; 10 parts of citronellol; 2.5 parts of limonene; 2.5 parts of elemenol; 5 parts of isolongifolene; 5 parts of α-cedrene; 2.5 parts of cedrol; 2.5 parts of α-cedrene; 2.5 parts of β-malolene; 2.5 parts of benzyl alcohol; and 2.5 parts of caryophyllene.

[0051] Mosquito repellent composition formula H: 10 parts of citronellal; 10 parts of geraniol; 10 parts of citronellol; 2.5 parts of limonene; 2.5 parts of elemenol; 10 parts of isolongifolene; 2.5 parts of α-ceryl alcohol; 2.5 parts of cedarwood; 2.5 parts of α-cedrene; 2.5 parts of β-malolene; 1.25 parts of benzyl alcohol; and 1.25 parts of caryophyllene.

[0052] The above mosquito repellent composition formulas E~H are prepared into mosquito repellent liquids according to the following formulas: 0.25 parts of mosquito repellent composition; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; using phosphate buffer to adjust the pH of the solution system to 7.2; and optimizing mosquito repellent liquids E~H are obtained respectively.

[0053]

[0054] Note: The effective protection time is an integer value (rounded off), and the average protection time is the average of the effective protection time of each subject (retain one decimal place).

[0055] As can be seen from Table 3, the mosquito repellent compositions contained in mosquito repellent liquids E to H have further improved mosquito repellent effects relative to the mosquito repellent compositions contained in mosquito repellent liquid A, and the harmony of the fragrance is also improved, while the mosquito repellent effect and fragrance coordination of mosquito repellent liquid H are obviously more advantageous. Therefore, the mosquito repellent composition in mosquito repellent liquid H is taken as the best formula, and after fine-tuning the amounts of its components, the mosquito repellent composition formula with the best effect is obtained, which consists of the following components in parts by weight: 8 to 17 parts of citronellal; 6 to 15 parts of geraniol; 4 to 10 parts of citronellol; 1.8 to 4 parts of limonene; 1.5 to 3 parts of elemenol; 7 to 15 parts of isolongifolene; 2.5 to 5 parts of α-cedrene; 2 to 5 parts of cedarwood; 1.2 to 2.5 parts of α-cedrene; 1.2 to 2.5 parts of β-malolene; 1.2 to 2.4 parts of benzyl alcohol; and 1 to 2.4 parts of caryophyllene.

[0056] Example 2

[0057] Mosquito repellent composition formula (measured in parts by mass): 17 parts of citronellal; 15 parts of geraniol; 5 parts of citronellol; 2 parts of limonene; 3 parts of elemenol; 14 parts of isolongifolene; 2.5 parts of α-berberine; 2.5 parts of cedarwood; 2.5 parts of α-cedrene; 2.5 parts of β-malolene; 1.2 parts of benzyl alcohol; 2.4 parts of caryophyllene.

[0058] Mosquito repellent composition formula (measured in parts by mass): 8 parts of citronellal; 6 parts of geraniol; 9 parts of citronellol; 2 parts of limonene; 3 parts of elemenol; 15 parts of isolongifolene; 5 parts of α-ceryl alcohol; 5 parts of cedarwood; 1.2 parts of α-cedrene; 1.2 parts of β-malolene; 1.2 parts of benzyl alcohol; 2.4 parts of caryophyllene.

[0059] Mosquito repellent composition formula (measured in parts by mass): The mosquito repellent composition is composed of the following components in parts by mass: 10 parts of citronellal; 12 parts of geraniol; 8 parts of citronellol; 2 parts of limonene; 2 parts of elemenol; 8 parts of isolongifolene; 3 parts of α-ceryl alcohol; 4 parts of cedarwood; 1.5 parts of α-cedrene; 2 parts of β-malolene; 1.5 parts of benzyl alcohol; and 1.5 parts of caryophyllene.

[0060] Mosquito repellent composition formula (measured by mass): 14 parts of citronellal; 10 parts of geraniol; 7 parts of citronellol; 2.5 parts of limonene; 2 parts of elemenol; 10 parts of isolongifolene; 3.5 parts of α-ceryl alcohol; 3.5 parts of cedarwood; 2 parts of α-cedrene; 2 parts of β-malolene; 2 parts of benzyl alcohol; 2 parts of caryophyllene.

[0061] Mosquito repellent composition formula (measured by mass): 14 parts of citronellal; 10 parts of geraniol; 7 parts of citronellol; 2.5 parts of limonene; 2 parts of elemenol; 10 parts of isolongifolene; 3.5 parts of α-cedrol; 0 parts of cedrol; 0 parts of α-cedrene; 2 parts of β-malolene; 2 parts of benzyl alcohol; 2 parts of caryophyllene.

[0062] Mosquito repellent composition formula (measured in parts by mass): 0 parts of citronellal; 10 parts of geraniol; 7 parts of citronellol; 2.5 parts of limonene; 2 parts of elemenol; 10 parts of isolongifolene; 3.5 parts of α-cedrene; 0 parts of cedrol; 0 parts of α-cedrene; 2 parts of β-malolene; 2 parts of benzyl alcohol; 2 parts of caryophyllene.

[0063] Example 3

[0064] Preparation of mosquito repellent:

[0065] The mosquito repellent composition formula in Example 1 ~ Add auxiliary agents and solvents to make mosquito repellent liquid.

[0066] The specific preparation process is as follows:

[0067] 1) stirring the raw materials of the mosquito repellent composition at room temperature to obtain a mixture;

[0068] 2) Add the mixture to an ethanol-water (or water) solution and stir thoroughly at room temperature until a transparent solution is obtained;

[0069] 3) Add an antioxidant to the transparent solution, stir evenly, then add a pH adjuster, stir evenly, and let it stand overnight to obtain a mosquito repellent liquid.

[0070] The formula of mosquito repellent liquid obtained by the above preparation method is as follows:

[0071] Mosquito repellent 1: Mosquito repellent composition formula 0.25 parts; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; and the pH of the solution system is adjusted to 7.2 using phosphate buffer.

[0072] Mosquito repellent 2: Mosquito repellent composition formula 0.25 parts; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; and the pH of the solution system is adjusted to 7.2 using phosphate buffer.

[0073] Mosquito repellent 3: Mosquito repellent composition formula 0.25 parts; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; and the pH of the solution system is adjusted to 7.2 using phosphate buffer.

[0074] Mosquito repellent 4: Mosquito repellent composition formula 0.25 parts; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; and the pH of the solution system is adjusted to 7.2 using phosphate buffer.

[0075] Mosquito repellent 5: Mosquito repellent composition formula 0.25 parts; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; and the pH of the solution system is adjusted to 7.2 using phosphate buffer.

[0076] Mosquito repellent 6: Mosquito repellent composition formula 0.25 parts; 300 parts of 8% ethanol aqueous solution; 0.01 parts of carotenoids; and the pH of the solution system is adjusted to 7.2 using phosphate buffer.

[0077] Mosquito repellent 7: mosquito repellent composition formula 0.1 parts; 300 parts of 5% ethanol aqueous solution; 0.005 parts of ascorbic acid; and the pH of the solution system was adjusted to 7.3 using phosphate buffer.

[0078] Mosquito repellent 8: mosquito repellent composition formula 0.5 parts; 300 parts of 10% ethanol aqueous solution; 0.015 parts of sodium isoascorbate; and the pH of the solution system was adjusted to 7.4 using phosphate buffer.

[0079] Example 4

[0080] The mosquito repellent liquids 1 to 8 in Example 3 were used to test the repellent effect:

[0081] The method for testing the repelling effect is the same as that in Example 1.

[0082] The specific test results are shown in Table 4:

[0083]

[0084] Note: The effective protection time is an integer value (rounded off), and the average protection time is the average of the effective protection time of each subject (retain one decimal place).

[0085] As can be seen from the table, the mosquito repellent effects of mosquito repellents 1 to 4 and 7 to 8 are more than 15 hours, and have good mosquito repellent effects. However, the component formulas of the mosquito repellent compositions of mosquito repellents 5 and 6 are not within the preferred range, and the mosquito repellent effects are significantly reduced. The proportion of the mosquito repellent composition used in mosquito repellent 8 is too high, and the relative cost is relatively high.

[0086] Example 5

[0087] Sensory evaluation test was conducted on mosquito repellents 1 to 8 in Example 3:

[0088] Experimental method: Take the mosquito repellent liquids of Examples 1 to 8, use absorbent paper with a width of 0.5 cm to 1.0 cm and a length of 10 cm to 15 cm as fragrance evaluation paper, and dip the mosquito repellent liquids of Examples 1 to 8 respectively to identify the odor. Five professional fragrance evaluation experts conducted fragrance evaluation, and the fragrance evaluation experimental results recognized by more than three fragrance evaluation experts are shown in Table 5.

[0089]

[0090] The active components of the mosquito repellent liquid of the present invention are all natural plant fragrance components, the fragrance is harmonious and non-irritating, which can make users easily accept it and improve the pleasure.

[0091] Example 6

[0092] The stability evaluation test was performed on the mosquito repellents 1 to 8 in Example 3:

[0093] Experimental method: 100 mL of each of the newly prepared mosquito repellent liquids 1 to 8 in Example 2 was taken, placed in a 150 mL glass bottle, sealed, and placed at 25° C. for 1 month. After one month, the mosquito repellent liquids 1 to 8 were all uniform transparent solutions without obvious black spots.

[0094] At the same time, the following mosquito repellents 9 and 10 were used as controls:

[0095] Mosquito repellent 9: mosquito repellent composition formula 0.25 parts; 300 parts of water; 0.01 parts of carotenoids; phosphate buffer solution was used to adjust the pH of the solution system to 7.2. After one month of storage, a small amount of colloid appeared in the mosquito repellent 9, and there were no obvious black spots, indicating that some components in the mosquito repellent had low solubility in water, and uneven dispersion was likely to occur if stored for too long, but it could be used normally after being shaken. This indicates that using a small amount of ethanol as a cosolvent significantly improves the dispersion effect.

[0096] Mosquito repellent 10: mosquito repellent composition formula 0.25 parts; 300 parts of 8% ethanol aqueous solution; 0 parts of carotenoids; phosphate buffer was used to adjust the pH of the solution system to 7.2; phosphate buffer was used to adjust the pH of the solution system to 7.3. After being placed for one month, the mosquito repellent 10 showed a transparent solution with a small amount of visible black spots, indicating that a few component raw materials were oxidized. Adding a small amount of antioxidant can prevent the active ingredients of the mosquito repellent from being oxidized by molecular oxygen dissolved in the mosquito repellent.

Claims

1. A mosquito repellent composition, characterized in that: It includes the following components by weight: Citronellal 8-17 parts; Geraniol 6-15 parts; Citronellol 4-10 parts; 1.8~4 parts of limonene; 1.5-3 parts of elemi alcohol; 7-15 parts of isolongifolene; 2.5-5 parts of α-pyridoxine; 2-5 parts of cedarwood; 1.2-2.5 parts of α-cedrene; 1.2-2.5 parts of β-malolene; 1.2-2.4 parts of benzyl alcohol; 1-2.4 parts of caryophyllene.

2. A mosquito repellent composition according to claim 1, characterized in that: It is composed of the following components by mass: Citronellal 10-15 parts; Geraniol 8-12 parts; Citronellol 6-8 parts; 2-3 parts of limonene; 2-2.5 parts of elemiol; 8-12 parts of isolongifolene; 3-4 parts of α-pyridoxine; 3-4 parts of cedarwood; 1.5-2 parts of α-cedrene; 1.5-2 parts of β-malolene; 1.5~2 parts of benzyl alcohol; 1.5~2 parts of caryophyllene.

3. A mosquito repellent liquid, characterized in that: The active ingredient is the mosquito repellent composition according to claim 1 or 2.

4. The mosquito repellent liquid according to claim 3, characterized in that: Also includes solvents and additives.

5. The mosquito repellent liquid according to claim 4, characterized in that: The solvent is water, or an alcohol aqueous solution composed of ethanol and water in a mass percentage of 5-10%:90-95%.

6. The mosquito repellent liquid according to claim 4, characterized in that: The auxiliary agent includes an antioxidant and / or a pH adjuster.

7. The mosquito repellent liquid according to claim 6, characterized in that: The antioxidant includes at least one of carotenoids, ascorbic acid, ascorbyl palmitate, erythorbic acid and sodium erythorbate.

8. The mosquito repellent liquid according to claim 6, characterized in that: The pH adjuster is a phosphate buffer.

9. A mosquito repellent liquid according to any one of claims 3 to 8, characterized in that: It includes the following components by weight: 0.1-0.5 parts of mosquito repellent composition; 300 parts of solvent; Antioxidant 0.005~0.015 parts; The pH adjuster is measured to adjust the pH of the mosquito repellent liquid to 7~7.5.

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