Compound liniment containing eucalyptus oil and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine agentia, and particularly discloses a compound liniment containing eucalyptus oil and a preparation method thereof.The preparation method comprises the steps that traditional Chinese medicine anti-inflammatory active ingredients extracted from traditional Chinese medicinal materials such as fructus cnidii and radix arnebiae seu lithospermi through enzymolysis water and eucalyptus oil form eucalyptus oil emulsion under the action of an additive, then the eucalyptus oil emulsion is added into gel, and the compound liniment containing eucalyptus oil is obtained. And preparing the compound liniment containing the eucalyptus oil. The compound liniment has anti-inflammatory, antibacterial, detumescence and itching relieving functions, is high in skin absorption capacity, and can effectively improve skin problems such as red and swollen skin, itching and dermatitis.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a compound liniment containing eucalyptus oil and a preparation method thereof. Background Art

[0002] With the improvement of science and technology, people are becoming more and more dependent on electronic products in their work and life. However, with long-term exposure to electronic products, people's skin condition is becoming worse and worse. For example, skin problems such as redness, itching, blisters, dermatitis, etc. are becoming more and more common. These common skin problems not only make people feel stinging, burning pain, and itching, affecting people's daily life and rest, but also cause skin bacterial infections and other diseases after severe scratching or picking, bringing inconvenience to people's lives.

[0003] Eucalyptus oil is a monoterpene oxide extracted from plants of the genus Eucalyptus or aromatic plants such as Asteraceae, Rosaceae, Zingiberaceae and Lauraceae. It is a colorless liquid at room temperature with a camphor-like odor. Eucalyptus oil has excellent biological activity and can play an anti-inflammatory, antibacterial, antioxidant and penetration-promoting role. It has received widespread attention in the fields of medicine, cosmetics and fragrances. Summary of the invention

[0004] The object of the present invention is to provide a compound ointment containing eucalyptus oil and a preparation method thereof. The compound ointment has anti-inflammatory, antibacterial, detumescent and antipruritic functions and can improve skin problems such as skin redness, itching, dermatitis, etc.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0006] A method for preparing a compound liniment containing eucalyptus oil, specifically comprising:

[0007] Eucalyptus oil and additives are mixed and stirred evenly to obtain a eucalyptus oil mixed solution, and the anti-inflammatory active ingredients of traditional Chinese medicine are slowly added to the eucalyptus oil mixed solution and continuously stirred to obtain a eucalyptus oil emulsion, and the eucalyptus oil emulsion is added to a gel base and stirred evenly to obtain a compound ointment containing eucalyptus oil.

[0008] As a limitation of the present invention, the amounts of the components in the compound ointment containing eucalyptus oil are, by weight, 18.0 to 20.0 parts of eucalyptus oil, 15.0 to 17.0 parts of additives, 55.0 to 65.0 parts of Chinese herbal anti-inflammatory active ingredients, and 24.0 to 30.0 parts of gel base.

[0009] As a limitation of the present invention, the preparation method of the anti-inflammatory active ingredient of traditional Chinese medicine is:

[0010] The Chinese medicinal materials are washed and crushed, mixed with deionized water, stirred evenly, and cellulase is added for enzymolysis. After the enzymolysis is completed, cicada slough, borneol, and alum are added, boiled over high heat, and then simmered over low heat for 60 to 90 minutes. After the simmering is completed, filter the residue, add deionized water to the filter residue, boil it again, and then simmer for 30 to 60 minutes. After the simmering is completed, filter the residue, combine the filtrates from the two filtrations, and concentrate them to obtain the anti-inflammatory active ingredients of the Chinese medicine.

[0011] As a limitation of the present invention, the amount of each component in the Chinese medicinal material is, by mass, 24-26 parts of Cnidium monnieri, 19-21 parts of Lithospermum officinale, 19-21 parts of Saposhnikovia divaricata, 14-16 parts of Schizonepeta tenuifolia, 9-11 parts of Plantago asiatica, 24-26 parts of Gardenia jasminoides, 29-31 parts of Mentha arvense, 4-6 parts of Aconitum carmichaelii, 29-31 parts of Chrysanthemum, 24-26 parts of Licorice, 24-26 parts of Kochia scoparia, 29-31 parts of Honeysuckle, 9-11 parts of Antidotes cochinchinensis, 14-16 parts of Juniperus chinensis, 4-6 parts of Tripterygium wilfordii, 9-11 parts of Red Cap Tripterygium wilfordii, and 14-16 parts of Tonkinensis leaf.

[0012] As a limitation of the present invention, the amounts of the components in the gel base are: 9 to 11 parts of quaternized chitosan, 2.5 to 4.5 parts of oxidized dextran, and 85 to 90 parts of deionized water.

[0013] As a limitation of the present invention, the preparation method of the quaternized chitosan is:

[0014] Mix deacetylated chitosan with 1% acetic acid solution, stir evenly to obtain a chitosan solution, mix 2,3-epoxypropyltrimethylammonium chloride with deionized water, stir evenly, add dropwise to the chitosan solution, stir continuously at 70-90°C, react for 8-12 hours, add acetone after the reaction is completed, stir evenly, let stand at 0-5°C for 22-26 hours, after the standing is completed, mix the obtained product with methanol, stir evenly, add ethanol and acetone to wash, filter out the precipitate, and freeze-dry to obtain quaternized chitosan.

[0015] As a limitation of the present invention, the preparation method of oxidized dextran is:

[0016] Dissolve dextran in deionized water, add sodium periodate, and react at 40-45°C with constant stirring for 1-3 hours. After the reaction is completed, add ethylene glycol for washing, filter, and freeze-dry to obtain oxidized dextran.

[0017] As a limitation of the present invention, the additive is composed of 13.5 to 14.5 parts of surfactant and 1.5 to 2.5 parts of auxiliary agent by weight.

[0018] As a limitation of the present invention, the surfactant is a combination of one or more of polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil, sodium lauryl sulfate, sodium lauryl sulfonate and Tween-80.

[0019] As a limitation of the present invention, the auxiliary agent is a combination of one or more of ethanol, isopropanol, n-butanol, glycerol, and polyethylene glycol-400.

[0020] Eucalyptus oil is cool in nature and spicy in taste, with a slightly camphor-like aroma. It has the effect of dispelling wind, relieving pain and relieving skin itching.

[0021] Cnidium monnieri is warm in nature and bitter in taste. It has the effects of dispelling wind, killing insects, drying dampness and warming the kidneys.

[0022] Lithospermum officinale is cold in nature and bitter in taste. It has the effects of promoting blood circulation, detoxifying and lubricating the intestines. "Compendium of Materia Medica" records that applying lithospermum officinale oil can treat insect bites.

[0023] Saposhnikovia divaricata is warm in nature and sweet in taste. It is effective in treating night sweats, constipation and migraine.

[0024] Schizonepeta is warm in nature and pungent in taste. It has the effects of dispelling wind, dispersing exterior pathogens and clearing away rashes.

[0025] Plantago seed is slightly cold in nature and sweet in taste. It has the effects of improving eyesight, clearing away heat, promoting diuresis and removing phlegm. "Shandong Traditional Chinese Medicine" records that it can treat eczema, blisters and children's head sores.

[0026] Gardenia is cold in nature and bitter in taste. It has the effects of reducing swelling, relieving pain, clearing away heat, detoxifying and being a diuretic. "Compendium of Materia Medica" records that burning gardenia, grinding it into powder, and applying it with oil can treat tinea between the eyebrows; mashing gardenia and white flour together and applying it to the painful area can relieve swelling and pain from injuries and fractures; mixing gardenia powder with egg white to make a concentrated solution and applying it can treat scalds and burns.

[0027] Mint is warm in nature and spicy in taste. It has the effects of dispelling wind, dispersing heat and stopping bleeding.

[0028] Chicken bone firewood is warm in nature and bitter in taste. It has the effects of warming the meridians and unblocking the collaterals, dispelling wind and dampness. According to the recipe in "Chinese Materia Medica", a proper amount of chicken bone firewood is mashed and applied on the surface of sores to prevent insect and fly bites.

[0029] Chrysanthemum is mild in nature and sweet in taste. It can treat wind-head, dizziness, swelling and pain in the head, loss of eyesight and tears, dead skin cells, and rheumatism.

[0030] Licorice is mild in nature and sweet in taste. It can treat sore throat caused by wind-heat, chronic cough caused by lung heat, mouth ulcers and back carbuncle. "Compendium of Materia Medica" records that using licorice decoction to wash the body three to five times a day can relieve warmth and itching in the genitals.

[0031] Kochia scoparia seeds are cold in nature and bitter in taste. They have the effects of clearing away heat and dampness, dispelling wind and relieving itching. They are used to treat painful urination, vaginal itching and leucorrhea, rashes, eczema, skin itching and other symptoms.

[0032] Honeysuckle is cold in nature and sweet in taste. It has the effects of clearing away heat and detoxifying, reducing swelling and relieving pain.

[0033] Baiducao is cold in nature and bitter in taste. It has the effects of detoxification, drying dampness and relieving itching. "Chinese Materia Medica" records that taking an appropriate amount of Baiducao and boiling it in water to wash can detoxify and relieve itching.

[0034] Juniper is cold in nature and bitter in taste. It has the effects of clearing heat, detoxifying, drying dampness and relieving itching. "Chinese Materia Medica" records that taking an appropriate amount of juniper, decocted in water and washing, can treat skin tinea and persistent low-grade fever.

[0035] Sanyaku is cold in nature and bitter in taste. The "Handbook of Commonly Used Chinese Herbal Medicines" records that Sanyaku can be crushed and applied externally to treat insect and snake bites, boils, bruises, sprains, eczema, dermatitis and other diseases.

[0036] Red hat top is cold in nature and bitter in taste. It has the effects of clearing away heat and detoxifying, drying dampness and relieving itching, cooling blood and stopping bleeding.

[0037] The leaves of the Chinese magnolia tree are warm in nature and bitter in taste. They have the effects of killing insects and treating ringworm, drying dampness and relieving itching, eliminating rashes and relieving pain.

[0038] Cicada molt is cold in nature and sweet in taste. It has the effects of dispersing wind-heat, clearing rashes and relieving itching, removing cataracts and improving eyesight. It is recorded in "Compendium of Materia Medica" that cicada molt should be ground into powder, one coin of it should be taken each time, and it should be washed down with sheep anus decoction, taken twice a day, to relieve skin itching.

[0039] Borneol is slightly cold in nature and bitter in taste. It has the effects of removing cataracts, improving eyesight, opening the orifices and dispersing heat, reducing swelling and relieving pain.

[0040] Alum is cold in nature and sour in taste. It has the effects of stopping bleeding, stopping diarrhea, and eliminating phlegm. It can be used externally to dry dampness and relieve itching.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] The active ingredients of Chinese herbal medicine are mixed with eucalyptus oil through additives such as polyoxyethylene castor oil to form eucalyptus oil emulsion, and the active ingredients of Chinese herbal medicine are dispersed and wrapped in eucalyptus oil. Eucalyptus oil contains 1,8-cineole, which can increase the disorder and fluidity of the fat alkyl chain of the stratum corneum, destroy the structure of keratin and extract lipids, thereby achieving transdermal absorption of drug ingredients. The combination of multiple active ingredients of Chinese herbal medicines has a synergistic effect and enhances the anti-inflammatory, antibacterial, detumescent and antipruritic effects of the compound ointment.

[0043] The gel is prepared with quaternized chitosan and oxidized dextran, and eucalyptus oil emulsion encapsulating the active ingredients of Chinese herbal medicine is added to the gel. This can increase the viscosity of the eucalyptus oil emulsion, prolong the drug retention time and the activity of the active ingredients, and make local administration more convenient. DETAILED DESCRIPTION

[0044] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] Eucalyptus oil (99%) was provided by Jiangsu Jiafu Pharmaceutical, cellulase (≥0.3 units / mg) was provided by Shanghai Merck, Chinese medicines such as Cnidium monnieri were sourced from Beijing Tong Ren Tang, deacetylated chitosan (95%) and dextran (98%) were provided by Xi’an Qi Yue, and the remaining materials were industrial products and available on the market.

[0046] Embodiment 1: A method for preparing a compound liniment containing eucalyptus oil, specifically:

[0047] Step 1: Preparation of anti-inflammatory active ingredients from traditional Chinese medicine

[0048] By mass, 315 parts of Chinese medicinal materials were washed and crushed, mixed with 500 parts of deionized water, stirred evenly, and 5 parts of cellulase were added for enzymolysis. After the enzymolysis was completed, 5 parts of cicada shells, 20 parts of borneol, and 10 parts of alum were added. The mixture was boiled over high heat, and then simmered over low heat for 75 minutes. After the simmering was completed, the mixture was filtered, and 500 parts of deionized water were added to the residue, boiled again, and simmered for 45 minutes. After the simmering was completed, the mixture was filtered, and the filtrates from the two filtrations were combined and concentrated to obtain the anti-inflammatory active ingredients of the Chinese medicine.

[0049] Step 2: Preparation of quaternized chitosan

[0050] By mass, 6 parts of deacetylated chitosan were mixed with 200 parts of 1% acetic acid solution, stirred evenly to obtain a chitosan solution, 18.75 parts of 2,3-epoxypropyltrimethylammonium chloride were mixed with 50 parts of deionized water, stirred evenly, and added dropwise to the chitosan solution, stirred continuously at 80°C, reacted for 10 hours, and after the reaction was completed, 78 parts of acetone were added, stirred evenly, and allowed to stand at 2°C for 24 hours. After the standing was completed, the obtained product was mixed with 79 parts of methanol, stirred evenly, ethanol and acetone were added for washing, the precipitate was filtered out, and freeze-dried to obtain quaternized chitosan.

[0051] Step 3: Preparation of oxidized dextran

[0052] By mass, 5 parts of dextran were dissolved in 250 parts of deionized water, 0.25 parts of sodium periodate were added, and the mixture was stirred continuously at 42°C for 2 hours. After the reaction was completed, ethylene glycol was added for washing, filtration, and freeze-drying to obtain oxidized dextran.

[0053] Step 4: Prepare the gel base

[0054] By weight, 10 parts of quaternized chitosan, 3.5 parts of oxidized dextran, and 60 parts of deionized water were mixed and stirred evenly, the pH was adjusted to 7 with triethanolamine, and the mixture was allowed to stand at room temperature for 10 hours to fully swell, thereby obtaining a gel base.

[0055] Step 5: Prepare the compound liniment containing eucalyptus oil

[0056] By mass, 19 parts of eucalyptus oil, 14 parts of polyoxyethylene castor oil and 2 parts of ethanol were mixed and stirred to obtain a eucalyptus oil mixed solution, 60 parts of traditional Chinese medicine anti-inflammatory active ingredients were slowly added to the eucalyptus oil mixed solution and stirred continuously to obtain a eucalyptus oil emulsion, and the obtained eucalyptus oil emulsion was added to 28 parts of gel base, stirred to obtain a compound ointment containing eucalyptus oil.

[0057] In parts by mass, in the present embodiment, the Chinese medicinal materials are composed of 25 parts of Cnidium monnieri, 20 parts of Lithospermum erythrorhizon, 20 parts of Saposhnikovia divaricata, 15 parts of Nepeta tenuifolia, 10 parts of Plantago asiatica, 25 parts of Gardenia jasminoides, 30 parts of Mentha suspensa, 5 parts of Aconitum carmichaelii, 30 parts of Chrysanthemum, 25 parts of Licorice, 25 parts of Kochia scoparia, 30 parts of Honeysuckle, 10 parts of Antidotes against Detoxification, 15 parts of Juniperus chinensis, 5 parts of Tripterygium wilfordii, 10 parts of Red Cap Tripterygium wilfordii, and 15 parts of Scutellaria baicalensis.

[0058] Embodiment 2: A method for preparing a compound liniment containing eucalyptus oil, specifically:

[0059] Step 1: Preparation of anti-inflammatory active ingredients from traditional Chinese medicine

[0060] By mass, 315 parts of Chinese medicinal materials were washed and crushed, mixed with 500 parts of deionized water, stirred evenly, and 5 parts of cellulase were added for enzymolysis. After the enzymolysis was completed, 5 parts of cicada shells, 20 parts of borneol, and 10 parts of alum were added. The mixture was boiled over high heat, and then simmered over low heat for 75 minutes. After the simmering was completed, the mixture was filtered, and 500 parts of deionized water were added to the residue, boiled again, and simmered for 45 minutes. After the simmering was completed, the mixture was filtered, and the filtrates from the two filtrations were combined and concentrated to obtain the anti-inflammatory active ingredients of the Chinese medicine.

[0061] Step 2: Preparation of quaternized chitosan

[0062] By mass, 6 parts of deacetylated chitosan were mixed with 200 parts of 1% acetic acid solution, stirred evenly to obtain a chitosan solution, 18.75 parts of 2,3-epoxypropyltrimethylammonium chloride were mixed with 50 parts of deionized water, stirred evenly, and added dropwise to the chitosan solution, stirred continuously at 80°C, reacted for 10 hours, and after the reaction was completed, 78 parts of acetone were added, stirred evenly, and allowed to stand at 2°C for 24 hours. After the standing was completed, the obtained product was mixed with 79 parts of methanol, stirred evenly, ethanol and acetone were added for washing, the precipitate was filtered out, and freeze-dried to obtain quaternized chitosan.

[0063] Step 3: Preparation of oxidized dextran

[0064] By mass, 5 parts of dextran were dissolved in 250 parts of deionized water, 0.25 parts of sodium periodate were added, and the mixture was stirred continuously at 42°C for 2 hours. After the reaction was completed, ethylene glycol was added for washing, filtration, and freeze-drying to obtain oxidized dextran.

[0065] Step 4: Prepare the gel base

[0066] By weight, 10 parts of quaternized chitosan, 3.5 parts of oxidized dextran, and 60 parts of deionized water were mixed and stirred evenly, the pH was adjusted to 7 with triethanolamine, and the mixture was allowed to stand at room temperature for 10 hours to fully swell, thereby obtaining a gel base.

[0067] Step 5: Prepare the compound liniment containing eucalyptus oil

[0068] By mass, 20 parts of eucalyptus oil, 14.5 parts of polyoxyethylene castor oil and 2.5 parts of ethanol were mixed and stirred to obtain a eucalyptus oil mixed solution, 65 parts of traditional Chinese medicine anti-inflammatory active ingredients were slowly added to the eucalyptus oil mixed solution and stirred continuously to obtain a eucalyptus oil emulsion, and the obtained eucalyptus oil emulsion was added to 30 parts of gel base, stirred to obtain a compound ointment containing eucalyptus oil.

[0069] In parts by mass, in the present embodiment, the Chinese medicinal materials are composed of 25 parts of Cnidium monnieri, 20 parts of Lithospermum erythrorhizon, 20 parts of Saposhnikovia divaricata, 15 parts of Nepeta tenuifolia, 10 parts of Plantago asiatica, 25 parts of Gardenia jasminoides, 30 parts of Mentha suspensa, 5 parts of Aconitum carmichaelii, 30 parts of Chrysanthemum, 25 parts of Licorice, 25 parts of Kochia scoparia, 30 parts of Honeysuckle, 10 parts of Antidotes against Detoxification, 15 parts of Juniperus chinensis, 5 parts of Tripterygium wilfordii, 10 parts of Red Cap Tripterygium wilfordii, and 15 parts of Scutellaria baicalensis.

[0070] Embodiment 3: A method for preparing a compound liniment containing eucalyptus oil, specifically:

[0071] Step 1: Preparation of anti-inflammatory active ingredients from traditional Chinese medicine

[0072] By mass, 315 parts of Chinese medicinal materials were washed and crushed, mixed with 500 parts of deionized water, stirred evenly, and 5 parts of cellulase were added for enzymolysis. After the enzymolysis was completed, 5 parts of cicada shells, 20 parts of borneol, and 10 parts of alum were added. The mixture was boiled over high heat, and then simmered over low heat for 75 minutes. After the simmering was completed, the mixture was filtered, and 500 parts of deionized water were added to the residue, boiled again, and simmered for 45 minutes. After the simmering was completed, the mixture was filtered, and the filtrates from the two filtrations were combined and concentrated to obtain the anti-inflammatory active ingredients of the Chinese medicine.

[0073] Step 2: Preparation of quaternized chitosan

[0074] By mass, 6 parts of deacetylated chitosan were mixed with 200 parts of 1% acetic acid solution, stirred evenly to obtain a chitosan solution, 18.75 parts of 2,3-epoxypropyltrimethylammonium chloride were mixed with 50 parts of deionized water, stirred evenly, and added dropwise to the chitosan solution, stirred continuously at 80°C, reacted for 10 hours, and after the reaction was completed, 78 parts of acetone were added, stirred evenly, and allowed to stand at 2°C for 24 hours. After the standing was completed, the obtained product was mixed with 79 parts of methanol, stirred evenly, ethanol and acetone were added for washing, the precipitate was filtered out, and freeze-dried to obtain quaternized chitosan.

[0075] Step 3: Preparation of oxidized dextran

[0076] By mass, 5 parts of dextran were dissolved in 250 parts of deionized water, 0.25 parts of sodium periodate were added, and the mixture was stirred continuously at 42°C for 2 hours. After the reaction was completed, ethylene glycol was added for washing, filtration, and freeze-drying to obtain oxidized dextran.

[0077] Step 4: Prepare the gel base

[0078] By weight, 10 parts of quaternized chitosan, 3.5 parts of oxidized dextran, and 60 parts of deionized water were mixed and stirred evenly, the pH was adjusted to 7 with triethanolamine, and the mixture was allowed to stand at room temperature for 10 hours to fully swell, thereby obtaining a gel base.

[0079] Step 5: Prepare the compound liniment containing eucalyptus oil

[0080] By weight, 18 parts of eucalyptus oil, 13.5 parts of polyoxyethylene castor oil and 1.5 parts of ethanol were mixed and stirred to obtain a eucalyptus oil mixed solution, 55 parts of traditional Chinese medicine anti-inflammatory active ingredients were slowly added to the eucalyptus oil mixed solution and stirred continuously to obtain a eucalyptus oil emulsion, and the obtained eucalyptus oil emulsion was added to 24 parts of gel base, stirred to obtain a compound ointment containing eucalyptus oil.

[0081] In parts by mass, in the present embodiment, the Chinese medicinal materials are composed of 25 parts of Cnidium monnieri, 20 parts of Lithospermum erythrorhizon, 20 parts of Saposhnikovia divaricata, 15 parts of Nepeta tenuifolia, 10 parts of Plantago asiatica, 25 parts of Gardenia jasminoides, 30 parts of Mentha suspensa, 5 parts of Aconitum carmichaelii, 30 parts of Chrysanthemum, 25 parts of Licorice, 25 parts of Kochia scoparia, 30 parts of Honeysuckle, 10 parts of Antidotes against Detoxification, 15 parts of Juniperus chinensis, 5 parts of Tripterygium wilfordii, 10 parts of Red Cap Tripterygium wilfordii, and 15 parts of Scutellaria baicalensis.

[0082] The following are comparative experiments based on Example 1, specifically Comparative Example 1, Comparative Example 2, and Comparative Example 3, as described below:

[0083] Comparative Example 1: This comparative example relates to a method for preparing a compound ointment containing eucalyptus oil, which differs from Example 1 only in that the Chinese medicine is not subjected to cellulase enzymolysis treatment, specifically:

[0084] Step 1: Preparation of anti-inflammatory active ingredients from traditional Chinese medicine

[0085] By mass, 315 parts of Chinese medicinal materials were washed and crushed, mixed with 500 parts of deionized water, stirred evenly, added with 5 parts of cicada shells, 20 parts of borneol, and 10 parts of alum, boiled over high heat, then simmered over low heat for 75 minutes, filtered after simmering, added with 500 parts of deionized water to the residue, boiled again, and simmered for 45 minutes, filtered after simmering, and the filtrates from the two filtrations were combined and concentrated to obtain the anti-inflammatory active ingredients of Chinese medicine.

[0086] Step 2: Preparation of quaternized chitosan

[0087] By mass, 6 parts of deacetylated chitosan were mixed with 200 parts of 1% acetic acid solution, stirred evenly to obtain a chitosan solution, 18.75 parts of 2,3-epoxypropyltrimethylammonium chloride were mixed with 50 parts of deionized water, stirred evenly, and added dropwise to the chitosan solution, stirred continuously at 80°C, reacted for 10 hours, and after the reaction was completed, 78 parts of acetone were added, stirred evenly, and allowed to stand at 2°C for 24 hours. After the standing was completed, the obtained product was mixed with 79 parts of methanol, stirred evenly, ethanol and acetone were added for washing, the precipitate was filtered out, and freeze-dried to obtain quaternized chitosan.

[0088] Step 3: Preparation of oxidized dextran

[0089] By mass, 5 parts of dextran were dissolved in 250 parts of deionized water, 0.25 parts of sodium periodate were added, and the mixture was stirred continuously at 42°C for 2 hours. After the reaction was completed, ethylene glycol was added for washing, filtration, and freeze-drying to obtain oxidized dextran.

[0090] Step 4: Prepare the gel base

[0091] By weight, 10 parts of quaternized chitosan, 3.5 parts of oxidized dextran, and 60 parts of deionized water were mixed and stirred evenly, the pH was adjusted to 7 with triethanolamine, and the mixture was allowed to stand at room temperature for 10 hours to fully swell, thereby obtaining a gel base.

[0092] Step 5: Prepare the compound liniment containing eucalyptus oil

[0093] By mass, 19 parts of eucalyptus oil, 14 parts of polyoxyethylene castor oil and 2 parts of ethanol were mixed and stirred to obtain a eucalyptus oil mixed solution, 60 parts of traditional Chinese medicine anti-inflammatory active ingredients were slowly added to the eucalyptus oil mixed solution and stirred continuously to obtain a eucalyptus oil emulsion, and the obtained eucalyptus oil emulsion was added to 28 parts of gel base, stirred to obtain a compound ointment containing eucalyptus oil.

[0094] In parts by mass, in the present embodiment, the Chinese medicinal materials are composed of 25 parts of Cnidium monnieri, 20 parts of Lithospermum erythrorhizon, 20 parts of Saposhnikovia divaricata, 15 parts of Nepeta tenuifolia, 10 parts of Plantago asiatica, 25 parts of Gardenia jasminoides, 30 parts of Mentha suspensa, 5 parts of Aconitum carmichaelii, 30 parts of Chrysanthemum, 25 parts of Licorice, 25 parts of Kochia scoparia, 30 parts of Honeysuckle, 10 parts of Antidotes against Detoxification, 15 parts of Juniperus chinensis, 5 parts of Tripterygium wilfordii, 10 parts of Red Cap Tripterygium wilfordii, and 15 parts of Scutellaria baicalensis.

[0095] Comparative Example 2: This comparative example relates to a method for preparing a compound ointment containing eucalyptus oil, which differs from Example 1 only in that the types and contents of Chinese herbal medicines are reduced. Specifically:

[0096] Step 1: Preparation of anti-inflammatory active ingredients from traditional Chinese medicine

[0097] By mass, 245 parts of Chinese medicinal materials were washed and crushed, mixed with 500 parts of deionized water, stirred evenly, and 5 parts of cellulase were added for enzymolysis. After the enzymolysis was completed, 5 parts of cicada shells, 20 parts of borneol, and 10 parts of alum were added. The mixture was boiled over high heat, and then simmered over low heat for 75 minutes. After the simmering was completed, the mixture was filtered, and 500 parts of deionized water were added to the residue, boiled again, and simmered for 45 minutes. After the simmering was completed, the mixture was filtered, and the filtrates from the two filtrations were combined and concentrated to obtain the anti-inflammatory active ingredients of the Chinese medicine.

[0098] Step 2: Preparation of quaternized chitosan

[0099] By mass, 6 parts of deacetylated chitosan were mixed with 200 parts of 1% acetic acid solution, stirred evenly to obtain a chitosan solution, 18.75 parts of 2,3-epoxypropyltrimethylammonium chloride were mixed with 50 parts of deionized water, stirred evenly, and added dropwise to the chitosan solution, stirred continuously at 80°C, reacted for 10 hours, and after the reaction was completed, 78 parts of acetone were added, stirred evenly, and allowed to stand at 2°C for 24 hours. After the standing was completed, the obtained product was mixed with 79 parts of methanol, stirred evenly, ethanol and acetone were added for washing, the precipitate was filtered out, and freeze-dried to obtain quaternized chitosan.

[0100] Step 3: Preparation of oxidized dextran

[0101] By mass, 5 parts of dextran were dissolved in 250 parts of deionized water, 0.25 parts of sodium periodate were added, and the mixture was stirred continuously at 42°C for 2 hours. After the reaction was completed, ethylene glycol was added for washing, filtration, and freeze-drying to obtain oxidized dextran.

[0102] Step 4: Prepare the gel base

[0103] By weight, 10 parts of quaternized chitosan, 3.5 parts of oxidized dextran, and 60 parts of deionized water were mixed and stirred evenly, the pH was adjusted to 7 with triethanolamine, and the mixture was allowed to stand at room temperature for 10 hours to fully swell, thereby obtaining a gel base.

[0104] Step 5: Prepare the compound liniment containing eucalyptus oil

[0105] By mass, 19 parts of eucalyptus oil, 14 parts of polyoxyethylene castor oil and 2 parts of ethanol were mixed and stirred to obtain a eucalyptus oil mixed solution, 60 parts of traditional Chinese medicine anti-inflammatory active ingredients were slowly added to the eucalyptus oil mixed solution and stirred continuously to obtain a eucalyptus oil emulsion, and the obtained eucalyptus oil emulsion was added to 28 parts of gel base, stirred to obtain a compound ointment containing eucalyptus oil.

[0106] By mass, in this embodiment, the Chinese medicinal materials are composed of 25 parts of Cnidium monnieri, 20 parts of Lithospermum erythrorhizon, 20 parts of Saposhnikovia divaricata, 15 parts of Schizonepeta tenuifolia, 10 parts of Plantago asiatica, 25 parts of Gardenia jasminoides, 30 parts of Menthus officinalis, 30 parts of Chrysanthemum, 25 parts of Licorice, 30 parts of Honeysuckle, and 15 parts of Nigella sativa.

[0107] Comparative Example 3: This comparative example relates to a method for preparing a compound ointment containing eucalyptus oil, which differs from Example 1 only in that no gel base is added to prepare the gel, specifically:

[0108] Step 1: Preparation of anti-inflammatory active ingredients from traditional Chinese medicine

[0109] By mass, 315 parts of Chinese medicinal materials were washed and crushed, mixed with 500 parts of deionized water, stirred evenly, and 5 parts of cellulase were added for enzymolysis. After the enzymolysis was completed, 5 parts of cicada shells, 20 parts of borneol, and 10 parts of alum were added. The mixture was boiled over high heat, and then simmered over low heat for 75 minutes. After the simmering was completed, the mixture was filtered, and 500 parts of deionized water were added to the residue, boiled again, and simmered for 45 minutes. After the simmering was completed, the mixture was filtered, and the filtrates from the two filtrations were combined and concentrated to obtain the anti-inflammatory active ingredients of the Chinese medicine.

[0110] Step 2: Prepare a compound liniment containing eucalyptus oil

[0111] 19 parts of eucalyptus oil, 14 parts of polyoxyethylene castor oil and 2 parts of ethanol were mixed by mass and stirred evenly to obtain a eucalyptus oil mixed solution. 60 parts of anti-inflammatory active ingredients of traditional Chinese medicine were slowly added to the eucalyptus oil mixed solution and stirred continuously to obtain a compound ointment containing eucalyptus oil.

[0112] In parts by mass, in the present embodiment, the Chinese medicinal materials are composed of 25 parts of Cnidium monnieri, 20 parts of Lithospermum erythrorhizon, 20 parts of Saposhnikovia divaricata, 15 parts of Nepeta tenuifolia, 10 parts of Plantago asiatica, 25 parts of Gardenia jasminoides, 30 parts of Mentha suspensa, 5 parts of Aconitum carmichaelii, 30 parts of Chrysanthemum, 25 parts of Licorice, 25 parts of Kochia scoparia, 30 parts of Honeysuckle, 10 parts of Antidotes against Detoxification, 15 parts of Juniperus chinensis, 5 parts of Tripterygium wilfordii, 10 parts of Red Cap Tripterygium wilfordii, and 15 parts of Scutellaria baicalensis.

[0113] Detection experiment:

[0114] According to the processes in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3, the compound ointment samples containing eucalyptus oil required for the detection experiment were prepared.

[0115] Skin toxicity test

[0116] Sixty healthy adult male rats aged 6 weeks were selected as experimental animals, each rat weighing 200-220g. They were randomly divided into 6 groups, with 10 rats in each group. The hair on both sides of the rats were shaved, and the compound ointment sample containing eucalyptus oil was applied to the skin on the same side of the rats, and glycerin was applied to the other side. The rats were observed after 24 hours.

[0117] In vitro antibacterial test

[0118] Two dermatophytes, Trichophyton mentagrophytes and Microsporum gypseum, were selected and inoculated on SDA medium. After culturing at room temperature for 7 days, the cultured colonies were dissolved in 2 mL of sterile saline, 1 mL of the upper homogenous solution was taken, and the bacterial suspension concentration was diluted to 1 × 10 with RMPI-1640 culture medium. 6 CFU / mL, 1 g of each of the 6 samples of compound ointments containing eucalyptus oil was taken and added to 50 mL of the diluted bacterial suspension, and shaken at room temperature for 4 h. After the shaking, the concentration of residual dermatophytes in the bacterial suspension was detected, and the inhibition rate of the compound ointment containing eucalyptus oil on the two dermatophytes was calculated.

[0119] Antipruritic test

[0120] 60 volunteers with skin redness, swelling and itching caused by mosquito bites were selected and divided into 6 groups. Each group was given a compound ointment sample containing eucalyptus oil and was told to apply the sample to the red, swollen and itchy areas. The average itching relief time and redness and swelling disappearance time of each group were recorded.

[0121]

[0122] Conclusion: According to the test data, the compound ointment containing eucalyptus oil prepared by the preparation method of Example 1 has no skin toxicity, and the inhibitory effect on two dermatophytes, Trichophyton mentagrophytes and Microsporum gypseum, is better than that of Comparative Example 1, Comparative Example 2, and Comparative Example 3. It is also better than Comparative Example 1, Comparative Example 2, and Comparative Example 3 in relieving skin itching and eliminating skin redness and swelling. The compound ointment containing eucalyptus oil of the present invention has the functions of anti-inflammatory, antibacterial, detumescence, and antipruritic, and can improve skin problems such as skin redness, swelling, itching, and dermatitis.

[0123] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

Claims

1. A method for preparing a compound liniment containing eucalyptus oil, characterized in that: Specifically: Eucalyptus oil and additives are mixed and stirred evenly to obtain a eucalyptus oil mixed solution, and the anti-inflammatory active ingredients of traditional Chinese medicine are slowly added to the eucalyptus oil mixed solution and continuously stirred to obtain a eucalyptus oil emulsion, and the eucalyptus oil emulsion is added to a gel base and stirred evenly to obtain a compound ointment containing eucalyptus oil.

2. A method for preparing a compound liniment containing eucalyptus oil according to claim 1, characterized in that: Calculated by weight, the dosage of each component in the compound ointment containing eucalyptus oil is: 18.0-20.0 parts of eucalyptus oil, 15.0-17.0 parts of additives, 55.0-65.0 parts of traditional Chinese medicine anti-inflammatory active ingredients, and 24.0-30.0 parts of gel base.

3. A method for preparing a compound liniment containing eucalyptus oil according to claim 1, characterized in that: The preparation method of the anti-inflammatory active ingredient of traditional Chinese medicine is as follows: The Chinese medicinal materials are washed and crushed, mixed with deionized water, stirred evenly, and cellulase is added for enzymolysis. After the enzymolysis is completed, cicada slough, borneol, and alum are added, boiled over high heat, and then simmered over low heat for 60 to 90 minutes. After the simmering is completed, filter the residue, add deionized water to the filter residue, boil it again, and then simmer for 30 to 60 minutes. After the simmering is completed, filter the residue, combine the filtrates from the two filtrations, and concentrate them to obtain the anti-inflammatory active ingredients of the Chinese medicine.

4. A method for preparing a compound liniment containing eucalyptus oil according to claim 3, characterized in that: The dosage of each component in the Chinese medicinal materials is as follows: 24-26 parts of Cnidium monnieri, 19-21 parts of Lithospermum officinale, 19-21 parts of Saposhnikovia divaricata, 14-16 parts of Nepeta tenuifolia, 9-11 parts of Plantago asiatica, 24-26 parts of Gardenia jasminoides, 29-31 parts of Mentha arvense, 4-6 parts of Aconitum carmichaelii, 29-31 parts of Chrysanthemum, 24-26 parts of Licorice, 24-26 parts of Kochia scoparia, 29-31 parts of Honeysuckle, 9-11 parts of Antidotes cochinchinensis, 14-16 parts of Juniperus chinensis, 4-6 parts of Tripterygium wilfordii, 9-11 parts of Red Cap Tripterygium wilfordii, and 14-16 parts of Tonkinensis leaf.

5. A method for preparing a compound liniment containing eucalyptus oil according to claim 1, characterized in that: The amounts of the components in the gel base are as follows: 9 to 11 parts of quaternized chitosan, 2.5 to 4.5 parts of oxidized dextran, and 85 to 90 parts of deionized water.

6. A method for preparing a compound liniment containing eucalyptus oil according to claim 5, characterized in that: The preparation method of the quaternized chitosan is: Mix deacetylated chitosan with 1% acetic acid solution, stir evenly to obtain a chitosan solution, mix 2,3-epoxypropyltrimethylammonium chloride with deionized water, stir evenly, add dropwise to the chitosan solution, stir continuously at 70-90°C, react for 8-12 hours, add acetone after the reaction is completed, stir evenly, let stand at 0-5°C for 22-26 hours, after the standing is completed, mix the obtained product with methanol, stir evenly, add ethanol and acetone to wash, filter out the precipitate, and freeze-dry to obtain quaternized chitosan.

7. A method for preparing a compound liniment containing eucalyptus oil according to claim 5, characterized in that: The preparation method of oxidized dextran is as follows: Dissolve dextran in deionized water, add sodium periodate, and react at 40-45°C with constant stirring for 1-3 hours. After the reaction is completed, add ethylene glycol for washing, filter, and freeze-dry to obtain oxidized dextran.

8. A method for preparing a compound liniment containing eucalyptus oil according to claim 1, characterized in that: The additive consists of 13.5 to 14.5 parts of surfactant and 1.5 to 2.5 parts of auxiliary agent by weight.

9. A method for preparing a compound liniment containing eucalyptus oil according to claim 8, characterized in that: The surfactant is a combination of one or more of polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil, sodium lauryl sulfate, sodium lauryl sulfonate and Tween-80.

10. The method for preparing a compound liniment containing eucalyptus oil according to claim 8, characterized in that: The auxiliary agent is a compound of one or more of ethanol, isopropanol, n-butanol, glycerol and polyethylene glycol-400.

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