Prescription of probiotics for regulating hyperglycemia and preparation method thereof

Abstract

The invention provides a formula for regulating hyperglycemia by probiotics and a preparation method of the formula, and relates to the technical field of the probiotics, the formula comprises two formulas, the formula I comprises the probiotics, hypoglycemic pepper, raw malt, burdock, snow pear and the like, and the formula II comprises the probiotics, the hypoglycemic pepper, the raw malt, the burdock, the snow pear and the like; and the formula II consists of inonotuso bliquus polysaccharide, schizophyllum commune, chromium yeast, soybean peptide powder and lactobacillus fermentation product powder. The two formulas can be taken independently, alternately or jointly, and are used for regulating the blood sugar level of patients with hyperglycemia or type 2 diabetes mellitus. The probiotic powder contains a plurality of strains with high activity and high viable count, and plays a role in digesting and absorbing basic metabolism. The natural components in the formula have a hypoglycemic effect, and play a role in improving the secretion function of pancreas islet beta cells, improving insulin sensitivity, delaying absorption of carbohydrates, helping produce saliva and slake thirst, diluting blood sugar and other mechanisms.

Description

Technical Field

[0001] The invention relates to the technical field of probiotics, and in particular to a formula for regulating hyperglycemia using probiotics and a preparation method thereof. Background Art

[0002] Traditional treatments for patients with high blood sugar or type 2 diabetes require long-term medication, which may be accompanied by drug side effects; Chinese medicine treatments vary from person to person, and the methods are diverse and not unified. Therefore, new, safer and more effective methods for preventing and treating high blood sugar or type 2 diabetes have important clinical and social significance.

[0003] In recent years, probiotic products, as a microecological preparation, have attracted widespread attention due to their multifaceted positive effects on human health. Probiotics can regulate the digestive and immune functions of the host by balancing the intestinal flora. Studies have shown that certain probiotics can improve insulin sensitivity, lower blood sugar levels, and have a definite effect on the prevention and treatment of hyperglycemia or type 2 diabetes.

[0004] Probiotics generally play a basic regulatory role in the human body. Once the human body is in a disease state such as hyperglycemia or type 2 diabetes, probiotics alone are not enough. Therefore, the development of probiotic compositions, as well as effective preparation and protection methods to enhance their blood sugar regulation function, has become a research hotspot in this field. Summary of the invention

[0005] To achieve the above objectives, the present invention is implemented by the following technical scheme: a probiotic formula for regulating hyperglycemia and a preparation method thereof, comprising the following steps:

[0006] Preparation method of formula 1: After cleaning the hypoglycemic pepper, raw malt, burdock and snow pear respectively, control the temperature at 50°C to 60°C to dry the cleaned raw materials until the moisture content is less than 5%, and then grind the dried raw materials into a fineness of more than 100 meshes by a grinder to obtain hypoglycemic pepper powder, raw malt powder, burdock powder and snow pear powder; mix the probiotic powder, hypoglycemic pepper powder, raw malt powder, burdock powder, snow pear powder and auxiliary materials evenly under sterile conditions, and obtain composition 1 after the mixed components.

[0007] Preparation method of formula 2: Chaga polysaccharide powder, Schizophyllum powder, yeast chromium powder, soybean peptide powder and lactic acid bacteria fermentation product powder are mixed evenly to obtain composition 2.

[0008] A probiotic formula for regulating hyperglycemia is composed of the following two formulas: Formula 1 is composed of 10-15 parts of probiotic powder, 5-15 parts of hypoglycemic pepper, 5-10 parts of raw malt, 5-10 parts of burdock, 5-10 parts of snow pear and 10-18 parts of auxiliary materials; Formula 2 is composed of 3-5 parts of chaga polysaccharide, 3-5 parts of schizophyllum, 0.5-2 parts of yeast chromium, 3-5 parts of soybean peptide powder, and 2-4 parts of lactic acid bacteria fermentation product powder. The two formulas can be taken alone, alternately or in combination, and are used to regulate blood sugar in people with hyperglycemia or type 2 diabetes.

[0009] The probiotic powder consists of 3 to 6 parts of bifidobacterium powder, 1 to 2 parts of rhamnosus lactobacillus powder, 1 to 2 parts of acidophilus lactobacillus powder, 1 to 2 parts of plant lactobacillus powder, 1 to 2 parts of casei powder, 1 to 2 parts of paracasei powder, 1 to 2 parts of salivarius powder and 1 to 2 parts of thermophilus streptococcus powder by weight.

[0010] The probiotic powder is obtained by strain culture, embedding treatment and spray drying of bifidobacterium, rhamnosus lactobacillus, acidophilus lactobacillus, plant lactobacillus plantarum, Lactobacillus casei, Lactobacillus paracasei, salivary combined lactobacillus and thermophilic streptococcus powder, ensuring that the number of viable bacteria in the probiotic powder is 10 8 ~10 10 CFU / g, and the water content is less than 5%, and the water activity is less than 0.1.

[0011] The pepper variety of the sugar-reducing pepper is Xiliang p1 sugar-reducing pepper.

[0012] The hypoglycemic pepper contains a natural α-glucosidase inhibitor, which can delay the absorption of carbohydrates. Raw malt contains maltose, which is a water-soluble dietary fiber that can reduce free fatty acids, triglycerides and other substances in the body; burdock is rich in burdock oligofructose; and the polyphenols contained in snow pear can lower blood sugar through various mechanisms. According to the theory of traditional Chinese medicine, the combination of the three medicinal and edible substances and hypoglycemic pepper mainly plays the role of regulating qi, blood, yin and yang, promoting fluid production and quenching thirst, diluting blood sugar, improving insulin sensitivity, protecting pancreatic beta cells, and delaying the absorption of carbohydrates, thereby achieving the effect of lowering blood sugar.

[0013] The auxiliary material consists of 5 to 9 parts of maltodextrin and 5 to 9 parts of lactose by weight.

[0014] The chaga polysaccharide can eliminate oxidation, inhibit tumor growth, reduce inflammation and insulin resistance in type 2 diabetes, and has the ability to stimulate the immune system.

[0015] The schizophyllum can effectively delay the absorption of carbohydrates to reduce blood sugar concentration, reduce total triglycerides, total cholesterol, low-density lipoprotein (LDL), increase high-density lipoprotein (HDL), and reduce liver indexes aspartate aminotransferase (SGOT), alanine aminotransferase (SGPT), and alkaline phosphatase (ALP).

[0016] The yeast chromium is an active component of the glucose tolerance factor, affects the metabolism of carbohydrates, lipids, proteins and nucleic acids, affects the body's ability to resist stress and disease, and is an essential trace element for the human body. It can activate insulin receptors and inhibit phosphorylated tyrosine phosphatase, thereby enhancing insulin sensitivity. It is rich in protein, carbohydrates and B vitamins.

[0017] The soybean peptide promotes pancreatic β cell proliferation, reduces inflammatory response, reduces pancreatic β cell apoptosis, and increases pancreatic islet cell sensitivity.

[0018] The preparation method of the lactic acid bacteria fermentation product powder comprises the following steps: dissolving skimmed milk, glucose and calcium carbonate and mixing them uniformly, sterilizing them at high temperature and high pressure, cooling them to 42° C. to obtain a symbiotic culture medium, inoculating lactic acid bacteria into the symbiotic culture medium, controlling the temperature at 36° C. to 42° C., fermenting them for 72 hours, centrifuging and separating after the fermentation to obtain lactic acid bacteria fermentation products, and drying them by high temperature spraying to obtain lactic acid bacteria fermentation product powder.

[0019] The lactic acid bacteria group is composed of bifidobacteria, lactobacilli, and streptococci. Bifidobacteria are preferably bifidobacteria longum; lactobacilli can be selected from one or more of Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus salivarius, Lactobacillus acidophilus, and Lactobacillus bulgaricus; preferably Lactobacillus bulgaricus; streptococci are preferably thermophilic streptococci, the inoculation amount of bifidobacteria is 3% to 6% of the mass of the culture medium, the inoculation amount of lactobacilli is 6% to 12% of the mass of the culture medium, the inoculation amount of streptococci is 1% to 2% of the mass of the culture medium, and the viable count of the above strains is 10 7 ~10 9 CFU / g.

[0020] Both Formula 1 and Formula 2 in the formula can be made into oral preparations, and their main preparation forms are any one of solid beverages, tablets, and capsules.

[0021] The present invention provides a probiotic formula for regulating hyperglycemia and a preparation method thereof. It has the following beneficial effects:

[0022] 1. The probiotic formula of the present invention contains a variety of probiotic powders and natural ingredients with hypoglycemic effects, such as hypoglycemic peppers, raw malt, burdock and snow pear; Chaga polysaccharide, Schizophyllum, yeast chromium, soybean peptides, and lactic acid bacteria fermentation products. The synergistic effect of these ingredients helps to improve the balance of intestinal microecology, promote the production of body fluids and quench thirst, dilute blood sugar, and improve insulin sensitivity. It may delay the absorption of sugars in the intestine by inhibiting the activity of α-glucosidase, thereby lowering blood sugar levels. This comprehensive effect provides a natural blood sugar regulation solution for patients with hyperglycemia or type 2 diabetes.

[0023] 2. The experimental results show that by using the probiotic formula of the present invention, the blood sugar levels of mice in test group 1, test group 2 and test group 3 decreased significantly after intervention. In particular, the blood sugar level of test group 3 dropped from 16.5±1.3mmol / L before intervention to 5.9±0.4mmol / L after intervention, showing a more obvious hypoglycemic effect compared with the control group (from 16.3±1.4mmol / L to 6.2±0.3mmol / L). This effect confirms the significant effect of the probiotic formula of the present invention in regulating blood sugar, and provides a treatment method for patients with hyperglycemia. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] The bifidobacterium in the specification and the examples is Bifidobacterium longum subsp. longum CICC 24934; Lactobacillus rhamnosus is Lactobacillus rhamnosus CICC 23119; Lactobacillus acidophilus is Lactobacillus acidophilus CICC 6085; Lactobacillus plantarum is Lactobacillus plantarum CICC 20326; Lactobacillus casei is Lactobacillus casei, and the strain deposit number is CICC 23185; Lactobacillus paracasei is Lactobacillus paracasei CICC 25261; Lactobacillus salivarius is Lactobacillus salivarius CICC 23174; the thermophilic Streptococcus is Streptococcus thermophilus CICC 6217; and the bulgaricus Lactobacillus is Lactobacillus delbrueckii subsp. bulgaricus CICC 6097.

[0026] Example 1

[0027] A probiotic formula for regulating hyperglycemia, wherein the components and their corresponding weight portions are shown in formula table 1, and the formula is prepared by the following steps:

[0028] The preparation method of formula 1 comprises the following steps: first, after cleaning the hypoglycemic pepper, raw malt, burdock and snow pear respectively, the temperature is controlled at 50°C to 60°C to dry the cleaned raw materials until the moisture content is less than 5%. Then, the dried raw materials are crushed to a fineness of more than 100 meshes by a grinder to obtain hypoglycemic pepper powder, raw malt powder, burdock powder and snow pear powder. Next, these powders are mixed evenly with probiotic powder and auxiliary materials under sterile conditions to obtain composition 1.

[0029] The preparation method of formula 2 comprises the following steps: mixing chaga polysaccharide powder, schizophyllum powder, yeast chromium powder, soybean peptide powder and lactic acid bacteria fermentation product powder uniformly to obtain composition 2.

[0030] The probiotic formula for regulating hyperglycemia prepared in Examples 2 to 5 has the same preparation method and operating conditions as Example 1. The difference from Example 1 is that the components and their corresponding weight portions are as shown in Tables 1 and 2 below the formula, Table 1 corresponds to composition 1, and Table 2 corresponds to composition 2.

[0031] Table 1

[0032]

[0033]

[0034] Table 2

[0035] Chaga Polysaccharide 5 3.9 4.5 4 3 Schizophyllum 3 4 3.6 5 2.5 Yeast Chromium 1.5 0.5 0.9 2 1.2 Lactic acid fermentation products 3.2 2 4.0 2.2 3.5 Soy peptide powder 3 5 3.8 4.2 3.5

[0036] Blood sugar relief experiment:

[0037] Forty healthy SPF-grade Kunming mice were used as experimental animals. The mice were housed in a barrier environment, with free access to food and water, and the bedding was changed every day. After one week of adaptive feeding, they were randomly divided into four groups, namely, a normal group, a hyperglycemia model group, experimental groups 1 to 3, and a control group. Each group had 10 mice, 5 males and 5 females, which were housed in separate cages. The grouping and treatment methods are detailed in Table 3. On the last day of the third week of the modeling period and after the end of the experimental period, the mice were tail-cut to draw blood, and the blood glucose levels of the mice were tested using a blood glucose meter, as detailed in Table 4.

[0038] Table 3

[0039]

[0040]

[0041]

[0042] Table 4

[0043]

[0044]

[0045] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A probiotic formula for regulating hyperglycemia, characterized in that: The formula is composed of the following two formulas: Formula 1 is composed of 10-15 parts of probiotic powder, 5-15 parts of hypoglycemic pepper, 5-10 parts of raw malt, 5-10 parts of burdock, 5-10 parts of snow pear and 10-18 parts of auxiliary materials; Formula 2 is composed of 3-5 parts of Chaga polysaccharide, 3-5 parts of Schizophyllum, 0.5-2 parts of yeast chromium, 3-5 parts of soybean peptide powder and 2-4 parts of lactic acid bacteria fermentation product powder. The two formulas can be taken alone, alternately or in combination, and are used for blood sugar regulation in patients with hyperglycemia or type 2 diabetes.

2. A probiotic formula for regulating hyperglycemia according to claim 1, characterized in that: The probiotic powder consists of 3 to 6 parts of bifidobacterium powder, 1 to 2 parts of rhamnosus lactobacillus powder, 1 to 2 parts of acidophilus lactobacillus powder, 1 to 2 parts of plant lactobacillus powder, 1 to 2 parts of casei powder, 1 to 2 parts of paracasei powder, 1 to 2 parts of salivarius powder and 1 to 2 parts of thermophilus streptococcus powder by weight.

3. The probiotic formula for regulating hyperglycemia according to claim 1, characterized in that: The probiotic powder is obtained by strain culture, embedding treatment and spray drying of bifidobacterium, rhamnosus lactobacillus, acidophilus lactobacillus, plant lactobacillus plantarum, Lactobacillus casei, Lactobacillus paracasei, salivary combined lactobacillus and thermophilic streptococcus, ensuring that the number of viable bacteria in the probiotic powder is 10 8 ~10 10 CFU / g, and the water content is less than 5%, and the water activity is less than 0.

1.

4. The probiotic formula for regulating hyperglycemia according to claim 1, characterized in that: The auxiliary material consists of 5 to 9 parts of maltodextrin and 5 to 9 parts of lactose by weight.

5. The probiotic formula for regulating hyperglycemia according to claim 1, characterized in that: The pepper variety of the sugar-reducing pepper is Xiliang p1 sugar-reducing pepper.

6. The probiotic formula for regulating hyperglycemia according to claim 1, characterized in that: Both Formula 1 and Formula 2 in the formula can be made into oral preparations, and their main preparation forms are any one of solid beverages, tablets, and capsules.

7. A method for preparing a probiotic formula for regulating hyperglycemia according to any one of claims 1 to 6, characterized in that: The preparation method of the formula 1 comprises: washing the hypoglycemic pepper, raw malt, burdock and snow pear respectively, drying them at a temperature of 50° C. to 60° C. until the moisture content is less than 5%, and then crushing the raw materials to a size of more than 100 meshes to obtain hypoglycemic pepper powder, raw malt powder, burdock powder and snow pear powder; uniformly mixing the hypoglycemic pepper powder, raw malt powder, burdock powder, snow pear powder, probiotic powder and auxiliary materials, and obtaining the composition 1 from the mixed components; The preparation method of the second formula is as follows: Chaga polysaccharide powder, Schizophyllum powder, yeast chromium powder, soybean peptide powder and lactic acid bacteria fermentation product powder are stirred and mixed uniformly to obtain composition 2.

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