Aspergillus niger composition and application thereof

Abstract

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to an aspergillus niger composition and application thereof. According to the composition disclosed by the invention, the aspergillus niger and the fertilizer synergist are combined for application, a synergistic effect is achieved, and the aspergillus niger also has high-concentration lead tolerance and lead repairing capability. The composition disclosed by the invention not only has phosphorus dissolving and straw degrading functions, but also can effectively improve the soil fertility and reduce the effectiveness of heavy metal lead. The composition disclosed by the invention plays an important role in promoting reutilization of phosphorus-containing solid wastes, promoting decomposition of straws, reducing emission of industrial and agricultural wastes, reducing the bioavailability of lead, maintaining the safety of an ecological environment and the like.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural microorganisms, in particular to an Aspergillus niger composition and application thereof. Background Art

[0002] Phosphate-solubilizing microorganisms can effectively promote the conversion of insoluble phosphorus in the soil into forms that can be absorbed and utilized by plants. The low molecular weight organic acids secreted by them can not only significantly reduce the pH value of the environment, but also react with Ca 2+ , Fe 3+ It can complex with metal ions such as phosphate and promote the release of phosphorus in insoluble phosphate. Aspergillus niger is a typical phosphate-solubilizing microorganism in the soil and has cellulase activity.

[0003] In the prior art, there is a technology that utilizes Aspergillus niger to degrade straw, increase the accumulation of soil organic matter, and achieve carbon fixation and emission reduction effects. However, in the application of degrading straw and repairing lead pollution, the effect of simply using Aspergillus niger in planting is not ideal. The growth of Aspergillus niger and the secretion of its organic acid are easily affected by various environmental factors, which in turn affects its application effect.

[0004] Therefore, how to provide a substance that can effectively improve the effects of Aspergillus niger on straw degradation, phosphorus dissolution and lead fixation has become an urgent problem to be solved in this field. Summary of the invention

[0005] In order to provide a method that can effectively achieve the effects of dissolving phosphorus, promoting straw degradation and fixing lead, the present invention provides an Aspergillus niger composition, which has the advantages of simple preparation method, high efficiency and environmental protection.

[0006] An Aspergillus niger composition, comprising the following components: Aspergillus niger and a fertilizer synergist;

[0007] The solid-liquid ratio of the fertilizer synergist and Aspergillus niger is 0.1-0.5 g:1 mL;

[0008] The concentration of Aspergillus niger in the composition is 2 to 3×10 8 CFU / mL.

[0009] Preferably, the Aspergillus niger includes Aspergillus niger AH-F-1-2, and the preservation number of the Aspergillus niger AH-F-1-2 is CGMCC No.23272.

[0010] Preferably, the fertilizer synergist includes any one or more of fulvic acid, alginic acid and glutamic acid.

[0011] The present invention also provides a method for preparing the Aspergillus niger composition as described above, comprising the following steps:

[0012] Inoculating the Aspergillus niger suspension into the PVK culture medium to obtain a PVK culture medium inoculated with the Aspergillus niger suspension;

[0013] A fertilizer synergist is added to the PVK culture medium inoculated with Aspergillus niger, and the culture is shaken to obtain the Aspergillus niger composition.

[0014] Preferably, the volume ratio of the Aspergillus niger suspension to the PVK medium is 1:100; the pH of the PVK medium is 7.0-7.2; the inoculation concentration of the Aspergillus niger suspension is (1-9)×10 7 CFU / mL.

[0015] Preferably, the volume ratio of the mass of the fertilizer synergist to the PVK culture medium is 0.1-0.5 g:100 mL.

[0016] Preferably, the shaking culture temperature is 28-30° C., and the shaking speed is 160-180 rpm.

[0017] The present invention also provides the use of the Aspergillus niger composition as described above or the Aspergillus niger composition prepared by the preparation method in any one or more of dissolving phosphorus, promoting straw degradation and fixing lead.

[0018] Preferably, when the Aspergillus niger composition is used for dissolving phosphorus and / or promoting straw degradation, the ratio of the Aspergillus niger suspension, the fertilizer synergist and the phosphorus-containing substance and / or the straw is 1 mL: 0.1-0.5 g: 0.5 g.

[0019] Preferably, the lead fixing comprises: fixing the lead after combining the Aspergillus niger composition with phosphorus-containing substances and / or straw.

[0020] The beneficial effects of the present invention are:

[0021] The invention provides an Aspergillus niger composition, which comprises Aspergillus niger and a fertilizer synergist; the solid-liquid ratio of the fertilizer synergist to Aspergillus niger is 0.1-0.5 g:1 mL; the concentration of the Aspergillus niger in the composition is 2-3×10 8 CFU / mL. The present invention applies Aspergillus niger and a fertilizer synergist in combination. The fertilizer synergist can promote the growth of Aspergillus niger or stimulate the metabolic activity of Aspergillus niger, and can stimulate the secretion of organic acid, especially oxalic acid, by Aspergillus niger, thereby promoting the degradation of straw or the release of phosphorus through acidification medium and chelation. In lead fixation, this process can not only promote the formation of lead oxalate minerals, but also generate more and more stable pyromorphite, thereby enhancing the repair effect of Aspergillus niger on lead pollution.

[0022] The present invention provides that the use of Aspergillus niger in combination with a fertilizer enhancer is an effective way to strengthen the utilization of phosphorus resources and improve the restoration of lead pollution, and plays an important role in promoting the reuse of phosphorus-containing solid waste, promoting the decay of straw, reducing the discharge of industrial and agricultural waste, reducing the biological effectiveness of lead, and maintaining the safety of the ecological environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0024] Figure 1 It is the colony map of the phosphate-dissolving fungi in Example 2;

[0025] Figure 2 This is a graph showing the effect of the fertilizer synergist combined with Aspergillus niger on the release of phosphorus in apatite in Example 8;

[0026] Figure 3 This is a graph showing the effect of the fertilizer synergist combined with Aspergillus niger on the release of phosphorus from straw in Example 9;

[0027] Figure 4 This is a graph showing the effect of the fertilizer synergist combined with Aspergillus niger on the microbial biomass in straw degradation in Example 9;

[0028] Figure 5 This is a graph showing the effect of the fertilizer synergist combined with Aspergillus niger on the release of oxalic acid during straw degradation in Example 9;

[0029] Figure 6 This is a graph showing the effect of the fertilizer synergist combined with Aspergillus niger on the straw degradation rate in Example 9;

[0030] Figure 7 This is the effect diagram of the fertilizer synergist combined with Aspergillus niger composite apatite or straw-fixed lead in Example 10.

[0031] Biological deposit information

[0032] Aspergillus niger strain AH-F-1-2, biological classification name: Aspergillus niger (Aspergillus niger), was deposited in the General Microbiology Center (CGMCC) of China Microorganism Culture Collection on September 30, 2021. The deposit address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the deposit number is CGMCC No. 23272. DETAILED DESCRIPTION

[0033] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0034] The invention provides an Aspergillus niger composition, comprising the following components: Aspergillus niger and a fertilizer synergist; the solid-liquid ratio of the fertilizer synergist to Aspergillus niger is 0.1-0.5 g:1 mL; the concentration of the Aspergillus niger in the composition is 2-3×10 8 CFU / mL.

[0035] As an embodiment, the solid-liquid ratio of the fertilizer synergist to Aspergillus niger of the present invention is 0.1-0.5 g:1 mL; as an embodiment, the solid-liquid ratio of the fertilizer synergist to Aspergillus niger can be any one of 0.1 g:1 mL, 0.2 g:1 mL, 0.3 g:1 mL, 0.4 g:1 mL and 0.5 g:1 mL; as an embodiment, the concentration of Aspergillus niger in the composition can be 2×10 8 CFU / mL, 2.1×10 8 CFU / mL, 2.2×10 8 CFU / mL, 2.3×10 8 CFU / mL, 2.4×10 8 CFU / mL, 2.58×10 8 CFU / mL, 2.6×10 8 CFU / mL, 2.7×10 8 CFU / mL, 2.8×10 8 CFU / mL, 2.9×10 8 CFU / mL and 3.0×10 8 CFU / mL; in a specific embodiment, the liquid ratio of the fertilizer synergist to Aspergillus niger is 0.4 g:1 mL, and the concentration of Aspergillus niger in the composition is 2.58×10 8 CFU / mL.

[0036] As an embodiment, the Aspergillus niger of the present invention includes Aspergillus niger AH-F-1-2; the Aspergillus niger AH-F-1-2 was screened from the wheat rhizosphere soil of the Anhui Agricultural University North Anhui Comprehensive Experiment Station and disclosed in patent CN116285999A. The colony of the Aspergillus niger AH-F-1-2 is charcoal black and flat, and the colony surface is granular with concentric ring patterns.

[0037] As an embodiment, the fertilizer synergist includes any one, two or three of fulvic acid, alginic acid and glutamic acid; in a specific embodiment, the fertilizer synergist is fulvic acid, and the weight portion of the fertilizer synergist is 0.4 parts.

[0038] The present invention also provides a method for preparing the Aspergillus niger composition described in the above technical scheme, comprising the following steps: inoculating an Aspergillus niger suspension into a PVK culture medium to obtain a PVK culture medium inoculated with the Aspergillus niger suspension; adding a fertilizer synergist to the PVK culture medium inoculated with the Aspergillus niger suspension, and performing shaking culture to obtain the Aspergillus niger composition.

[0039] The present invention inoculates the Aspergillus niger suspension into the PVK culture medium to obtain the PVK culture medium inoculated with the Aspergillus niger suspension. As an embodiment, the volume ratio of the Aspergillus niger suspension to the PVK culture medium is 1:100; As an embodiment, the inoculation concentration of the Aspergillus niger suspension is (1-9)×10 7 CFU / mL; As an embodiment, the inoculation concentration of the Aspergillus niger suspension includes 1×10 7 CFU / mL, 2×10 7 CFU / mL, 3×10 7 CFU / mL, 4×10 7 CFU / mL, 5×10 7 CFU / mL, 6×10 7 CFU / mL, 7×10 7 CFU / mL, 8×10 7 CFU / mL and 9×10 7 CFU / mL. As an embodiment, the PVK medium includes the following raw materials in weight: 10 parts of glucose, 0.5 parts of ammonium sulfate, 0.2 parts of sodium chloride, 0.25 parts of magnesium sulfate heptahydrate, 0.03 parts of iron sulfate heptahydrate, 0.2 parts of potassium chloride, 0.03 parts of manganese sulfate tetrahydrate and 1000 parts of deionized water; in a specific embodiment, the volume ratio of the Aspergillus niger suspension to the PVK medium is 1:100; the pH of the PVK medium is 7.1, and the PVK medium includes the following raw materials: 10g of glucose, 0.5g of ammonium sulfate, 0.2g of sodium chloride, 0.25g of magnesium sulfate heptahydrate, 0.03g of iron sulfate heptahydrate, 0.2g of potassium chloride, 0.03g of manganese sulfate tetrahydrate and 1000mL of deionized water; the pH of the PVK medium is 7.0-7.2; as an embodiment, the pH of the PVK medium can be any one of 7.0, 7.1 and 7.2.

[0040] After obtaining the PVK culture medium inoculated with the Aspergillus niger suspension, the present invention adds a fertilizer synergist to the PVK culture medium inoculated with the Aspergillus niger suspension, shakes and cultures, and obtains the Aspergillus niger composition. As an embodiment, the mass ratio of the fertilizer synergist to the volume of the PVK culture medium is 0.1-0.5 g:100 mL; as another embodiment, the mass ratio of the fertilizer synergist to the volume of the PVK culture medium can be any one of 0.1 g:100 mL, 0.2 g:100 mL, 0.3 g:100 mL, 0.4 g:100 mL and 0.5 g:100 mL; as an embodiment, the temperature of the shaking culture is 28°C-30°C, and the shaking speed is 160-180 rp m; as another embodiment, the temperature of the shaking culture can be any one of 28°C, 29°C and 30°C; as another embodiment, the shaking speed of the shaking culture can be any one of 160rpm, 170rpm and 180rpm; in a specific embodiment, the present invention adds a fertilizer synergist to the PVK culture medium and shakes the culture, the inoculation ratio of the fertilizer synergist to the PVK culture medium is 0.4g:100mL, the temperature of the shaking culture is 29°C, and the shaking speed is 170rpm.

[0041] The present invention also provides the use of the composition as described in any one of the above items or the composition prepared by any one of the above preparation methods in any one or more of dissolving phosphorus, promoting straw degradation and fixing lead.

[0042] As an embodiment, the application of the Aspergillus niger composition in phosphorus dissolution includes the following steps: after mixing the Aspergillus niger composition with a phosphorus-containing substance, shaking and culturing, the phosphorus dissolution effect can be achieved; as an embodiment, the phosphorus-containing substance includes apatite. As an embodiment, when applied to the phosphorus dissolution, the ratio of the Aspergillus niger suspension to the fertilizer synergist and the phosphorus-containing substance is 1mL:0.1~0.5g:0.5g. As an embodiment, the ratio of the Aspergillus niger suspension to the fertilizer synergist and the phosphorus-containing substance can be any one of 1mL:0.1g:0.5g, 1mL:0.2g:0.5g, 1mL:0.3g:0.5g, 1mL:0.4g:0.5g and 1mL:0.5g:0.5g. As an embodiment, the shaking culture condition is 160-180rpm shaking culture for 5 days; in a specific embodiment, the application of the Aspergillus niger composition in phosphorus dissolution includes the following steps: after the Aspergillus niger composition and apatite are evenly mixed, shaking culture is carried out at 170rpm for 5 days to achieve the phosphorus dissolution effect; the ratio of the Aspergillus niger suspension to the fertilizer enhancer and the phosphorus-containing substance is 1mL:0.4g:0.5g.

[0043] As another embodiment, the application of the Aspergillus niger composition in phosphorus dissolution can also combine the preparation of the Aspergillus niger composition with the application of phosphorus dissolution, comprising the following steps: mixing Aspergillus niger, a fertilizer synergist, a PVK culture medium and a phosphorus-containing substance and then shaking and culturing to achieve a phosphorus dissolution effect; as an embodiment, the shaking culture condition is shaking and culturing at 160 to 180 rpm for 5 days; in a specific embodiment, the application of the Aspergillus niger composition in phosphorus dissolution comprises the following steps: after uniformly mixing Aspergillus niger, a fertilizer synergist, a PVK culture medium and apatite, shaking and culturing at 170 rpm for 5 days to achieve a phosphorus dissolution effect.

[0044] As an embodiment, the application of the Aspergillus niger composition in promoting straw degradation comprises the following steps: the Aspergillus niger composition is mixed with straw to achieve the effect of promoting straw degradation; as an embodiment, the shaking culture condition is 160-180rpm shaking culture for 5 days; as an embodiment, the straw can be rice straw; as an embodiment, when applied to the promotion of straw degradation, the ratio of the Aspergillus niger suspension to the fertilizer synergist and the straw is 1mL:0.1-0.5g:0.5g. As an embodiment, the ratio of the Aspergillus niger suspension to the fertilizer synergist and the straw can be any one of 1mL:0.1g:0.5g, 1mL:0.2g:0.5g, 1mL:0.3g:0.5g, 1mL:0.4g:0.5g and 1mL:0.5g:0.5g. In a specific embodiment, the application of the Aspergillus niger composition in promoting straw degradation includes the following steps: after the Aspergillus niger composition and rice straw are evenly mixed, shaking and culturing at 170rpm for 5 days to achieve the effect of promoting straw degradation; the ratio of the Aspergillus niger suspension to the fertilizer enhancer and rice straw is 1mL:0.4g:0.5g.

[0045] As another embodiment, the application of the Aspergillus niger composition in promoting straw degradation can also combine the preparation of the Aspergillus niger composition with the application in promoting straw degradation, comprising the following steps: mixing Aspergillus niger, fertilizer enhancer, PVK culture medium and straw and then shaking and culturing to achieve the effect of promoting straw degradation; as an embodiment, the shaking culture condition is shaking and culturing at 160-180rpm for 5d; in a specific embodiment, the application of the Aspergillus niger composition in promoting straw degradation comprises the following steps: after uniformly mixing Aspergillus niger, fertilizer enhancer, PVK culture medium and straw, shaking and culturing at 170rpm for 5d to achieve the effect of promoting straw degradation.

[0046] As an embodiment, the application of the Aspergillus niger composition in lead fixing comprises the following steps: mixing the Aspergillus niger composition with a phosphorus-containing substance and / or straw to obtain a mixture, and mixing the mixture with a lead-containing substance to achieve a lead fixing effect; as an embodiment, the lead-containing substance comprises lead nitrate; in a specific embodiment, the application of the Aspergillus niger composition in lead fixing comprises the following steps: uniformly mixing the Aspergillus niger, a fertilizer enhancer and straw, and mixing the mixture with lead nitrate after the straw is degraded to achieve a lead fixing effect; in another specific embodiment, the application of the Aspergillus niger composition in lead fixing comprises the following steps: uniformly mixing the Aspergillus niger composition with apatite, and mixing the mixture with lead nitrate after the apatite is dissolved to achieve a lead fixing effect.

[0047] As another embodiment, the application of the Aspergillus niger composition in lead fixing comprises the following steps: mixing Aspergillus niger, a fertilizer synergist, a phosphorus-containing substance and a lead-containing substance, wherein the phosphorus-containing substance can increase the stability of lead fixation, and the Aspergillus niger can promote the release of phosphorus in the phosphorus-containing substance, and the released phosphorus can further react with lead to form a very stable lead-containing mineral, thereby achieving the lead fixing effect; as another embodiment, the application of the Aspergillus niger composition in lead fixing comprises the following steps: mixing Aspergillus niger, a fertilizer synergist, straw and a lead-containing substance, thereby achieving the lead fixing effect; in a specific embodiment, the application of the Aspergillus niger composition in lead fixing comprises the following steps: mixing Aspergillus niger, a fertilizer synergist, apatite and lead nitrate to achieve the lead fixing effect; in another specific embodiment, the application of the Aspergillus niger composition in lead fixing comprises the following steps: mixing Aspergillus niger, a fertilizer synergist, crop straw and lead nitrate to achieve the lead fixing effect.

[0048] In summary, the purpose of the present invention is to provide an Aspergillus niger composition prepared by combining different fertilizer synergists with Aspergillus niger, and the application of the composition in promoting the release of phosphorus from apatite, promoting the degradation of straw, and the use of composite phosphate and straw for lead fixation. Aspergillus niger can secrete a large amount of oxalic acid (H2C2O4), which can promote the dissolution of phosphorus in phosphate rock or increase the degradation rate of cellulose, promote the composting and degradation of straw through acidified medium, and can also combine with straw to reduce the biological effectiveness of lead in the environment.

[0049] In order to further illustrate the present invention, an anti-wrinkle and firming repairing composition containing sturgeon caviar extract and its application provided by the present invention are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0050] Example 1 Preparation of culture medium

[0051] Prepare PVK medium, PDA medium and PDB medium according to the raw material components shown below;

[0052] PVK medium: glucose 10 g, ammonium sulfate 0.5 g, sodium chloride 0.2 g, magnesium sulfate heptahydrate 0.25 g, iron sulfate heptahydrate 0.03 g, potassium chloride 0.2 g, manganese sulfate tetrahydrate 0.03 g, deionized water 1000 mL, pH 7.1.

[0053] PDA culture medium: Wash and peel fresh potatoes, weigh 200g, cut into small pieces, add water and boil for 25min, filter with eight layers of gauze, add 20g glucose and 15-20g agar powder while hot, stir evenly, and after the culture medium cools down, add water to 1000mL.

[0054] PDB medium: 46 g potato dextrose medium, 1000 mL deionized water, pH 7.0.

[0055] The preparation methods of the culture medium are all as follows: dissolving the raw materials in deionized water and mixing well.

[0056] Example 2 Spore suspension preparation

[0057] The Aspergillus niger strain AH-F-1-2 disclosed in patent CN116285999A was used, the Aspergillus niger strain was inoculated on PDA medium, and cultured in an incubator at 28° C. for 5 days to form spores;

[0058] The PDA medium containing Aspergillus niger spores was taken out, and the medium was washed with sterile water on a sterile operating table. The fungal spores on the PDA medium were scraped off with a sterile inoculating loop, and mixed with the sterile water used to wash the medium to obtain a spore suspension. The spore suspension was filtered through three layers of gauze to remove broken hyphae fragments to obtain a spore liquid. The spore concentration in the spore liquid was measured with a hemocytometer, and the concentration of the spore liquid was diluted to 9×10 7 CFU / mL, and Aspergillus niger suspension was obtained.

[0059] Embodiment 4 A kind of Aspergillus niger composition

[0060] The composition comprises 1 part of Aspergillus niger; 0.1 part of fertilizer synergist;

[0061] The Aspergillus niger suspension was prepared according to the method described in Example 2, except that the final concentration of the Aspergillus niger suspension was 1×10 7 CFU / mL;

[0062] The fertilizer synergist is fulvic acid, and the inoculation concentration of fulvic acid is 1 g / L.

[0063] Example 5 A kind of Aspergillus niger composition

[0064] The composition comprises 1 part of Aspergillus niger; 0.3 part of fertilizer synergist;

[0065] The Aspergillus niger suspension was prepared according to the method described in Example 2, except that the final concentration of the Aspergillus niger suspension was 5×10 7 CFU / mL;

[0066] The fertilizer synergist is glutamic acid, and the inoculation concentration of glutamic acid is 3 g / L.

[0067] Example 6 A kind of Aspergillus niger composition

[0068] The composition comprises 1 part of Aspergillus niger; 0.5 part of fertilizer synergist;

[0069] The Aspergillus niger suspension is prepared according to the method described in Example 2;

[0070] The fertilizer synergist is alginate, and the inoculation concentration of alginate is 5 g / L.

[0071] Example 7 Preparation method of an Aspergillus niger composition

[0072] The preparation method comprises the following steps:

[0073] Weigh Aspergillus niger and fertilizer enhancer according to proportion and set aside;

[0074] Inoculating the Aspergillus niger into a PVK medium, the volume ratio of Aspergillus niger to the PVK medium is 1:100, and the pH of the PVK medium is 7.1;

[0075] A fertilizer synergist is added to the PVK culture medium, the inoculation ratio of the fertilizer synergist to the PVK culture medium is 0.3 g:100 mL, and the culture is shaken at 29° C. and 170 rpm to obtain a composition.

[0076] Example 8 Application of fertilizer synergist combined with Aspergillus niger in promoting the release of phosphorus in apatite

[0077] In this example, the concentration of Aspergillus niger in the composition is 2.58×10 8 CFU / mL; the inoculation amount of Aspergillus niger liquid was 1% of the volume of PVK culture medium; in PVK culture medium, ammonium sulfate was the only nitrogen source.

[0078] Apatite sample processing: After the apatite is collected, it is first crushed and then ground into powder using a ball mill. After passing through a 100-mesh sieve, it is stored for later use.

[0079] The experimental method is as follows: add 100mL PVK culture medium into a 250mL conical flask and sterilize at 121℃ for 20min; add Aspergillus niger and 0.5g apatite powder, and then divide into 18 groups for treatment, with three replicates in each group, and add 0g fulvic acid, 0.1g fulvic acid, 0.2g fulvic acid, 0.3g fulvic acid, 0.4g fulvic acid, 0.5g fulvic acid; 0g alginate, 0.1g alginate, 0.2g alginate, 0.3g alginate, 0.4g alginate, 0.5g alginate; 0g glutamic acid, 0.1g glutamic acid, 0.2g glutamic acid, 0.3g glutamic acid, 0.4g glutamic acid, 0.5g glutamic acid.

[0080] The above culture medium was cultured in a shaking incubator at 28°C and 180 rpm for 5 days. Samples were taken on the 5th day to observe and measure the growth of the strain. The available phosphorus content in each treatment culture medium was measured by ICP-OES. The results are as follows: Figure 2 And as shown in Table 1.

[0081] Table 1 Phosphorus solubilization of apatite by different fertilizer synergists combined with Aspergillus niger

[0082]

[0083] Note: The letters after the numbers represent the differences in phosphorus dissolving effects of different concentrations of fertilizer enhancers. The same letters represent the same level.

[0084] From Table 1 and Figure 2 It can be seen from the content that compared with the group without adding fertilizer synergists, the group with fertilizer synergists (alginic acid, fulvic acid or glutamic acid) can promote the release of phosphorus in phosphate rock by Aspergillus niger, and the effects are significantly different. Among them, after 5 days of cultivation, 4g / L glutamic acid combined with Aspergillus niger has the best effect on promoting phosphorus release from apatite, and the phosphorus content of this group of solutions is the highest.

[0085] Example 9 Effect of fertilizer synergist combined with Aspergillus niger on rice straw degradation

[0086] In this example, the concentration of Aspergillus niger in the composition is 2.58×10 8 CFU / mL; the inoculation amount of Aspergillus niger liquid was 1% of the volume of PVK culture medium; in PVK culture medium, ammonium sulfate was the only nitrogen source.

[0087] Rice straw sample processing: After drying the rice straw, cut it into 2 cm segments and set aside.

[0088] The experimental method is as follows: add 100mL PVK culture medium into a 250mL conical flask and sterilize at 121℃ for 20min; add Aspergillus niger solution and 0.5g rice straw, and then divide into 18 groups for treatment, with three replicates in each group, and add 0g fulvic acid, 0.1g fulvic acid, 0.2g fulvic acid, 0.3g fulvic acid, 0.4g fulvic acid, 0.5g fulvic acid; 0g alginate, 0.1g alginate, 0.2g alginate, 0.3g alginate, 0.4g alginate, 0.5g alginate; 0g glutamic acid, 0.1g glutamic acid, 0.2g glutamic acid, 0.3g glutamic acid, 0.4g glutamic acid, 0.5g glutamic acid.

[0089] The above culture medium was cultured in a shaking incubator at 28°C and 180 rpm for 5 days. Samples were taken on the 5th day to observe and measure the growth of the strain. The available phosphorus content in each treatment culture medium was measured by ICP-OES. The results are as follows: Figure 3 As shown in Table 2; the straw degradation rate was observed and measured, and the results were as follows Figure 4 As shown in Table 3; The biomass of Aspergillus niger in different groups was measured, and the results were as follows Figure 5 As shown in Table 4; the amount of oxalic acid secretion from straw was measured, and the results were as follows Figure 6 As shown in Table 5.

[0090] Table 2 Release of phosphorus from straw by fertilizer synergists combined with Aspergillus niger

[0091]

[0092] Note: The letters after the numbers represent the differences in the effects of different concentrations of fertilizer enhancers on phosphorus release from straw. The same letters represent the same level.

[0093] From Table 2 and Figure 3 It can be seen that compared with the group without adding fertilizer enhancers (alginate / fulvic acid / glutamic acid), the group adding fertilizer enhancers can promote the release of phosphorus in straw by Aspergillus niger, and there is a significant difference between the effects.

[0094] Table 3 Degradation rate of straw by fertilizer synergist combined with Aspergillus niger

[0095]

[0096] Note: The letters after the numbers represent the differences in the effects of different concentrations of fertilizer synergists combined with Aspergillus niger on promoting straw degradation. The same letters represent the same level.

[0097] From Table 3 and Figure 4 It can be seen that compared with the group without adding fertilizer synergists (alginate / fulvic acid / glutamic acid), the group adding fertilizer synergists can promote the degradation of straw by Aspergillus niger, and there is a significant difference between the effects.

[0098] Table 4 Effect of fertilizer synergist combined with Aspergillus niger on the biomass of Aspergillus niger in straw degradation

[0099]

[0100] Note: The letters after the numbers represent the difference between the effects of different concentrations of fertilizer enhancers combined with Aspergillus niger on increasing the biomass of Aspergillus niger. The same letters represent the same level.

[0101] From Table 4 and Figure 5 It can be seen that, except for the group adding 0.5 g glutamic acid, the other groups adding fertilizer enhancers can promote the growth of Aspergillus niger and increase the biomass of Aspergillus niger.

[0102] Table 5 Oxalic acid secretion of fertilizer synergists combined with Aspergillus niger to degrade straw

[0103]

[0104] Note: The letters after the numbers represent the differences in oxalic acid secretion during the straw degradation process of different concentrations of fertilizer enhancers combined with Aspergillus niger. The same letters represent the same level.

[0105] From Table 5 and Figure 6 It can be seen that the addition of glutamic acid can promote the secretion of oxalic acid by Aspergillus niger, while the addition of fulvic acid will inhibit the secretion of oxalic acid by Aspergillus niger, and alginate has little effect on the secretion of oxalic acid by Aspergillus niger. This is because Aspergillus niger secretes many types of organic acids, and oxalic acid is the main organic acid that causes straw degradation and phosphorus release. After the addition of fulvic acid, the secretion of oxalic acid decreased, indicating that compared with glutamic acid and alginate, fulvic acid is weaker in promoting the secretion of oxalic acid by Aspergillus niger, but fulvic acid may also stimulate the secretion of other organic acids by Aspergillus niger, resulting in the result of promoting straw degradation. But overall, the effect of fulvic acid is weaker than that of glutamic acid and alginate. Among them, the content of oxalic acid released is the highest when 5g / L of glutamic acid is added.

[0106] Example 10 Effect of fertilizer synergist combined with Aspergillus niger composite phosphate on lead fixation

[0107] Based on the results in Example 8 and Example 9, the amount of fertilizer synergist added in this experiment was limited to 0.4 g; the concentration of Aspergillus niger in the composition in this example was 2.58×10 8 CFU / mL; the inoculation amount of Aspergillus niger liquid was 1% of the volume of PVK culture medium; in PVK culture medium, ammonium sulfate was the only nitrogen source, and by adding 0.16 g of lead nitrate to 100 mL of PVK culture medium, a PVK culture medium with an initial lead mass concentration of 1000 mg / L was obtained.

[0108] The experimental method is as follows: add 100mL PVK culture medium into a 250mL conical flask and sterilize at 121℃ for 20min; add Aspergillus niger, and then divide into 8 groups for treatment, with three replicates in each group, namely: no fertilizer enhancer + 0.5g apatite, addition of 0.4g fulvic acid + 0.5g apatite, addition of 0.4g alginate + 0.5g apatite, addition of 0.4g glutamic acid + 0.5g apatite, no fertilizer enhancer + 0.5g rice straw, addition of 0.4g fulvic acid + 0.5g rice straw, addition of 0.4g alginate + 0.5g rice straw, addition of 0.4g glutamic acid + 0.5g rice straw.

[0109] The above culture medium was cultured in a shaking incubator at 28°C and 180 rpm for 5 days. Samples were taken on the 5th day and the lead content in each treated filtrate was determined by ICP-OES. The results are shown in Table 6 and Figure 7 shown.

[0110] Table 6 Effects of fertilizer synergists combined with Aspergillus niger on lead fixation in composite apatite and rice straw

[0111]

[0112] Note: The letters after the numbers represent the difference in lead content between different fertilizer synergists combined with Aspergillus niger to remove lead solution. The same letters represent the same level.

[0113] From Table 6 and Figure 7 It can be seen that compared with not adding fertilizer enhancers, adding fertilizer enhancers can significantly reduce the lead content in the solution, among which the combination of alginate and apatite combined with Aspergillus niger has the best lead fixation effect.

[0114] From the above examples, it can be concluded that the Aspergillus niger composition of the present invention produces a synergistic effect by using Aspergillus niger in combination with a fertilizer synergist, which can promote the release of phosphorus in phosphorus-containing substances and promote the degradation of straw; the Aspergillus niger composition used with phosphorus-containing substances and / or straw also has the effect of fixing lead. The present invention provides that the use of Aspergillus niger in combination with a fertilizer synergist is an effective way to strengthen the utilization of phosphorus resources and improve the remediation of lead pollution, and plays an important role in promoting the reuse of phosphorus-containing solid waste, promoting the decay of straw, reducing the discharge of industrial and agricultural waste, reducing the biological effectiveness of lead, and maintaining the safety of the ecological environment.

[0115] The above description is only a preferred embodiment of the present invention. It should be pointed out that a person skilled in the art can make several improvements without departing from the principle of the present invention, and these improvements should also be regarded as within the protection scope of the present invention.

Claims

1. An Aspergillus niger composition, characterized in that The Aspergillus niger composition comprises the following components: Aspergillus niger and a fertilizer synergist; The solid-liquid ratio of the fertilizer synergist and Aspergillus niger is 0.1-0.5 g:1 mL; The concentration of Aspergillus niger in the composition is 2 to 3×10 8 CFU / mL.

2. The Aspergillus niger composition according to claim 1, characterized in that The Aspergillus niger includes Aspergillus niger AH-F-1-2, and the preservation number of the Aspergillus niger AH-F-1-2 is CGMCC No.23272.

3. The Aspergillus niger composition according to claim 1, characterized in that The fertilizer synergist includes any one or more of fulvic acid, alginic acid and glutamic acid.

4. The method for preparing the Aspergillus niger composition according to claim 1, characterized in that: The steps include: Inoculating the Aspergillus niger suspension into the PVK culture medium to obtain a PVK culture medium inoculated with the Aspergillus niger suspension; A fertilizer synergist is added to the PVK culture medium inoculated with Aspergillus niger, and the culture is shaken to obtain the Aspergillus niger composition.

5. The preparation method according to claim 4, characterized in that: The volume ratio of the Aspergillus niger suspension to the PVK culture medium is 1:100; the pH of the PVK culture medium is 7.0-7.2; the inoculation concentration of the Aspergillus niger suspension is (1-9)×10 7 CFU / mL.

6. The preparation method according to claim 4, characterized in that: The volume ratio of the mass of the fertilizer synergist to the PVK culture medium is 0.1-0.5 g:100 mL.

7. The preparation method according to claim 4, characterized in that: The shaking culture temperature is 28-30° C., and the shaking speed is 160-180 rpm.

8. Use of the Aspergillus niger composition according to any one of claims 1 to 3 or the Aspergillus niger composition prepared by the preparation method according to any one of claims 4 to 7 in any one or more of dissolving phosphorus, promoting straw degradation and fixing lead.

9. The use according to claim 8, characterized in that When the Aspergillus niger composition is used for dissolving phosphorus and / or promoting straw degradation, the ratio of the Aspergillus niger suspension, the fertilizer synergist and the phosphorus-containing substance and / or the straw is 1 mL: 0.1-0.5 g: 0.5 g.

10. The use according to claim 9, characterized in that: The lead fixing method comprises: fixing the lead by combining the Aspergillus niger composition with phosphorus-containing substances and / or straw.

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