Non-human primate spinal cord half-cut injury model manufacturing method

Abstract

The invention discloses a manufacturing method of a non-human primate spinal cord half-cut injury model. The spinal cord and the dura mater are exposed in sequence, the dura mater is cut off with the assistance of a dura mater drag hook frame, a positioning guide needle is arranged in the middle axis of the spinal cord through a stereotaxic instrument and a positioning guide needle installed on the stereotaxic instrument, a dura mater protection sheet is arranged, and spinal cord tissue is cut off downwards along a sliding groove of the positioning guide needle through a microscalpel. And a drainage tube is used for indwelling an epidural subfascia for drainage, and a nursing garment is worn, so that manufacturing of a non-human primate spinal cord semi-cutting injury model is realized. According to the method, the modeling consistency and the modeling success rate are ensured.

Description

Technical Field

[0001] The invention relates to the technical field of non-human primate spinal cord injury models, and in particular to a method for preparing a non-human primate spinal cord hemisection injury model. Background Art

[0002] In medical experiments targeting a specific disease, collecting research data through experimental animals is an extremely important part, such as research on spinal cord injury. For spinal cord injury, the most suitable experimental animal is the monkey. Among them, in order to study the mechanism of spinal cord injury and its treatment methods, creating an animal spinal cord injury model through monkeys is a crucial step.

[0003] At present, there are mainly the following animal spinal cord injury models: including the hemostatic clamp method spinal cord compression injury model, the heavy object falling method spinal cord impact injury model, the spinal cord transection injury model, the spinal cord hemisection injury model, the balloon compression injury model, etc.

[0004] Among them, the existing hemisection injury model of the spinal cord is usually made manually. The first step is to cut the corresponding positions of the back in sequence until the dura mater is cut; the second step is to directly cut the spinal cord in half with a scalpel; the third step is to suture the cut dura mater and the back. However, it is difficult to ensure the consistency and success rate of modeling by only using the above three steps to make a hemisection injury model of the spinal cord. There is a lack of positioning before cutting, it is difficult to ensure the accuracy of the hemisection, there is a lack of safety during the cutting process, it is easy to damage the dura mater, and there is a lack of post-suturing care and cerebrospinal fluid leakage prevention and treatment methods, which affects the healing of the wound. Summary of the invention

[0005] The purpose of the present invention is to provide a method for preparing a non-human primate spinal cord hemisection injury model.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A method for preparing a non-human primate spinal cord hemisection injury model comprises the following steps:

[0008] The first step is to expose the spinal cord and dura mater. Specifically, the spinous processes of T9 to T11 are located on the surface of the body, a 5 cm incision is made in the posterior midline, and the layers are separated downwards to expose the T10 lamina. The T10 spinous process and ligament are removed in sequence, the T10 lamina is removed by drilling, and then the yellow ligament is removed using microsurgical instruments to expose the spinal cord.

[0009] The second step is to cut the dura mater with the help of a dura mater retractor. Specifically, a vertical incision is selected when opening the dura mater, so that the dura mater can be sutured after the modeling is completed, thereby minimizing the degree of cerebrospinal fluid leakage. In order to reduce the damage to the spinal cord tissue when cutting the dura mater, a dura mater retractor is used to assist in this step. The dura mater retractor pulls the dura mater to create a gap between the dura mater and the spinal cord tissue, thereby avoiding damage to the spinal cord during the process of cutting the dura mater.

[0010] The third step is to use a stereotaxic instrument and a positioning guide needle installed on the stereotaxic instrument to insert the positioning guide needle into the central axis of the spinal cord. The positioning guide needle has a slide groove to limit the movement trajectory of the microsurgical knife. The insertion position of the guide needle is accurately located by the stereotaxic instrument to ensure the accuracy of the spinal cord hemisection range. The positioning guide needle is then driven down by the stereotaxic instrument to pierce the spinal cord.

[0011] The fourth step is to insert a dura mater protective sheet. Before the hemisection operation, a dura mater protective sheet is placed on the side. The dura mater protective sheet is inserted between the dura mater and the spinal cord tissue, and the insertion depth is such that the tip of the dura mater protective sheet touches the positioning guide needle.

[0012] Step 5: Use a microsurgical knife to cut the spinal cord tissue downward along the guide pin's slide until the tip of the blade cuts the dura mater protective sheet below. At this time, move the microsurgical knife outward to ensure that the spinal cord tissue on one side is completely cut off.

[0013] Step 6: After the spinal cord hemisection modeling is completed, the dura mater is sutured watertightly and a drainage tube is placed under the epidural fascia to reduce the risk of complications caused by postoperative cerebrospinal fluid leakage.

[0014] The seventh step is to complete the modeling until the drainage tube is removed. Considering that the experimental animals may pull out the drainage tube spontaneously, they need to wear nursing clothes and fix the drainage bag with the nursing clothes. Then, by observing the incision and drainage volume, the drainage tube should be removed in time.

[0015] As a further technical solution of the present invention: the positioning guide needle is a straight needle, and the cross-sections of the needle body and the needle tip of the positioning guide needle are both crescent-shaped, and the inner sides of the needle body and the needle tip are connected to form the sliding groove.

[0016] As a further technical solution of the present invention: the dura mater retractor frame comprises two retractor frames and two fixing rods, the two retractor frames are arranged opposite to each other, each retractor frame is formed with a support frame column and a support head integrally connected to the top of the support frame column, the support head is detachably installed with a hook module for lifting the dura mater in the middle thereof and has mounting arms extending outwardly on both sides thereof, the mounting arms are provided with through openings passing through the front and back sides thereof and first threaded locking holes connected to the through openings; each fixing rod is correspondingly installed between the front and rear corresponding mounting arms of the two retractor frames, and its two ends pass through the through openings; each first threaded locking hole is provided with a first locking screw for the first locking screw to pass into the through opening to press against and lock the fixing rod, and the two retractor frames are connected and fixed by the two fixing rods.

[0017] As a further technical solution of the present invention: the bracket head is provided with a groove in the middle thereof for installing the hook module; the hook module comprises a hook seat detachably installed in the groove and a hook body installed on the hook seat.

[0018] As a further technical solution of the present invention: the hook seat is provided with a hook-mounting groove penetrating through the front and back sides thereof in the middle section of its top surface, and the hook-mounting groove is provided with a second threaded locking hole in the middle section of its bottom surface; the hook body is formed with a main arm end inserted into the hook-mounting groove and a hook arm section integrally connected to the main arm end for lifting the dura mater; the main arm end is provided with an adjusting strip opening penetrating through the top and bottom surfaces thereof, and the adjusting strip opening is provided with a second locking screw corresponding to the second threaded locking hole, so that the second locking screw is inserted into the second threaded locking hole to press and lock the main arm end to fix the hook body on the hook seat, and the adjusting strip opening can move forward and backward along the second locking screw by loosening the second locking screw to adjust the relative position of the hook arm section and the hook seat.

[0019] As a further technical solution of the present invention: the dura mater protective sheet is in the shape of a hook, forming an inner hook section and an outer arm section integrally connected to the inner hook section, the recessed portion of the inner hook section is a medullary groove for dura mater incision, and the dura mater protective sheet extends the inner hook section into the incision.

[0020] As a further technical solution of the present invention: the nursing clothing is formed with a bra and an abdominal belt connected to the bottom of the bra, the bra is worn on the chest and back, and the abdomen and back are covered with the abdominal belt; the abdominal belt is formed with an abdominal section for fitting the abdomen, a strap section connected to one side of the abdominal section for fitting one side of the waist, the back and the other side of the waist in sequence, and a tie strap section connected to the other side of the abdominal section that can be detachably overlapped with the tie strap section, and a first overlapping structure is provided between the tie strap section and the tie strap section; the drainage bag is arranged on the inner layer of the tie strap section.

[0021] As a further technical solution of the present invention: the bra is provided with a front chest section in the middle part, two back sections are provided opposite to each other on both sides thereof, and an arm section is provided between each back section and the front chest section; the arm section is provided with a cuff opening, and a second overlapping structure is provided between the two back sections, so that when the bra is worn on the chest and back, the front chest section fits the front chest, the arms are respectively passed through the cuff openings, and the two back sections are overlapped and connected at the back through the second overlapping structure.

[0022] As a further technical solution of the present invention: the outer fabrics of the bra and the belly band are both cut-resistant fabrics, and the belly band is provided with a gauze pad on its inner layer.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention proposes a method for preparing a non-human primate spinal cord hemisection injury model, which sequentially exposes the spinal cord and dura mater, cuts the dura mater with the assistance of a dura mater retractor, inserts a positioning guide needle into the central axis of the spinal cord using a stereotaxic instrument and a positioning guide needle installed on the stereotaxic instrument, inserts a dura mater protective sheet, uses a microsurgical knife to cut the spinal cord tissue downward along the slide groove of the positioning guide needle, sutures the dura mater watertightly, and uses a drainage tube to retain drainage under the epidural fascia and wears a nursing garment, thereby achieving the preparation of a non-human primate spinal cord hemisection injury model, ensuring the consistency of modeling and the success rate of modeling, improving the accuracy of hemisection, avoiding damage to the dura mater during hemisection, and performing post-suturing care with a nursing garment, thereby promoting wound healing. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is an overall picture of the dura mater retractor.

[0025] Figure 2 Schematic diagram of connecting and fixing two hook brackets to fix the rod.

[0026] Figure 3 A schematic diagram of the bracket head.

[0027] Figure 4 Schematic diagram of the stereotaxic instrument and the positioning guide needle.

[0028] Figure 5 This is a diagram of the placement of the dura mater protective sheet.

[0029] Figure 6 A cross-sectional view of the positioning guide needle.

[0030] Figure 7 This is a schematic diagram of a nursing garment.

[0031] Figure 8 This is a structural diagram of a nursing garment.

[0032] Fig. 9 This is a diagram of how to wear nursing clothes. DETAILED DESCRIPTION

[0033] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the protection scope of the present invention.

[0034] A method for preparing a non-human primate spinal cord hemisection injury model comprises the following steps:

[0035] The first step is to expose the spinal cord and dura mater. Specifically, the spinous processes of T9 to T11 are located on the body surface, a 5 cm incision is made in the middle of the back, and the layers are separated downwards to expose the T10 lamina. The T10 spinous process and ligament are removed in sequence, and the T10 lamina is removed by a drill. Then, the yellow ligament is removed with microsurgical instruments to expose the spinal cord. During this process, the range of lamina removal must be expanded as much as possible to provide surgical space for the subsequent opening of the dura mater and hemisection of the spinal cord.

[0036] In the second step, the SCI contusion model does not require the dura mater to be cut. The modeling process can be achieved by adjusting the impact parameters outside the dura mater through the LISA impactor to perform spinal cord impact to achieve the purpose of SCI contusion. However, the SCI hemisection model is different. This modeling method requires an incision on one side of the spinal cord. For the mouse SCI hemisection model, because mice have less cerebrospinal fluid, the spinal cord is cut together with the dura mater, and cerebrospinal fluid leakage will not occur. However, non-human primates are the same as humans. When the dura mater is opened, cerebrospinal fluid continues to flow out of the dura mater. If it is not treated specifically, it will cause cerebrospinal fluid leakage, which will cause subsequent complications such as wound infection and wound non-healing, and ultimately lead to modeling failure. Please refer to Figure 1 , Figure 2 and Figure 3 In order to reduce the risk of the above problems, the dura mater is incised with the assistance of the dura mater retractor 10. Specifically, a vertical incision is selected when opening the dura mater to facilitate suturing the dura mater after modeling, thereby minimizing the degree of cerebrospinal fluid leakage. In order to reduce the damage to the spinal cord tissue when incising the dura mater, the dura mater retractor 10 is assisted in this step. The dura mater retractor 10 pulls the dura mater to create a gap between the dura mater and the spinal cord tissue, thereby avoiding damage to the spinal cord during the process of incising the dura mater.

[0037] The third step is to consider the stability and accuracy of the SCI model in addition to the relevance of clinical diseases. The SCI contusion model relies on the LISA impact instrument, and the degree of impact can be adjusted by the instrument to maintain the stability and accuracy of the model. However, the factor that has the greatest impact on the stability and accuracy of the SCI hemisection model is the damage range of the spinal cord hemisection. Accurately controlling the range of spinal cord transection during each modeling process through the instrument is of great significance for stabilizing this modeling method. Please refer to Figure 4 , Figure 5 and Figure 6 In order to solve this problem, a stereotaxic instrument 21 and a positioning guide needle 22 installed on the stereotaxic instrument 21 are used to insert the positioning guide needle 22 into the central axis of the spinal cord, and a slide groove 221 is provided in the positioning guide needle 22 to limit the moving trajectory of the microsurgical knife. The insertion position of the guide needle 22 is accurately located by the stereotaxic instrument 21, thereby ensuring the accuracy of the spinal cord hemisection range, and then the stereotaxic instrument 21 drives the positioning guide needle 22 to descend to pierce the spinal cord.

[0038] The fourth step is to insert the dura mater protective sheet 30. Although the positioning guide needle 22 can be used to accurately adjust the range of the spinal cord tissue incision, the dura mater on one side or even the ventral side will inevitably be damaged during the lateral incision. In order to avoid this problem, the dura mater protective sheet 30 needs to be placed laterally before the half-incision operation. The insertion position of the dura mater protective sheet 30 is between the dura mater and the spinal cord tissue, and the insertion depth is when the tip of the dura mater protective sheet 30 touches the positioning guide needle 22.

[0039] The fifth step is to use a microsurgical knife to cut the spinal cord tissue downward along the slide groove 221 of the positioning guide needle 22 until the tip of the blade cuts the dura mater protective sheet 30 below. At this time, the microsurgical knife is cut outward to ensure that the spinal cord tissue on one side is completely cut off.

[0040] Step 6. Clinically, the treatment strategies for cerebrospinal fluid leakage include suture or material closure of the fistula, drug inhibition of cerebrospinal fluid production, subarachnoid shunt, pressure dressing of the surgical area, and adjustment of the patient's position. Although there are many different treatment methods, watertight suture during surgery is still the key to dealing with this problem. At the same time, with the continuous advancement of medical science and technology, many biomaterials such as fibrin glue, hydrogel and U-shaped clips are constantly coming out, and more and more repair methods are being used clinically. But no matter what method is adopted, effectively closing the dura mater rupture and preventing cerebrospinal fluid leakage are of great significance for preventing postoperative complications and improving clinical prognosis. Therefore, after the spinal cord hemisection modeling is completed, this embodiment selects watertight suture of the dura mater and drainage tube 41 for drainage under the epidural fascia to reduce the risk of complications caused by postoperative cerebrospinal fluid leakage.

[0041] Step 7. Dura mater injury often leads to postoperative cerebrospinal fluid leakage, making the treatment of this problem complicated and difficult. If not handled properly, it may lead to many complications such as non-healing of the incision, dura mater pseudocysts, and intracranial hypotension. In severe cases, it may cause intraspinal infection and meningitis, which are life-threatening. In the past, it was believed that in patients with cerebrospinal fluid leakage, indwelling drainage in the surgical area may lead to cerebrospinal fluid skin fistula, pseudocyst formation, and even intracranial hemorrhage due to excessive drainage. Wolff et al. recommend that patients with good suture or watertight repair should be drained for 48 hours after surgery. If the repair is not done or the watertight repair cannot be achieved, drainage is not recommended to avoid excessive drainage. Clinical experience also suggests that long-term indwelling drainage tubes41 increase the risk of retrograde infection, and may also delay the time of leaving bed and increase the risk of bed-related complications such as aspiration pneumonia and deep vein thrombosis of the lower extremities.

[0042] Recently, more and more studies have shown that after watertight suture repair of the dura mater, the retention of extradural subfascial drainage is not correlated with the occurrence of cerebrospinal fluid skin fistula, pseudocyst formation, subdural hemorrhage and other problems. There is a risk of hematoma compression in the early stage after lumbar surgery. Conventional retention drainage is beneficial to clear hematoma, eliminate dead space and promote healing. In addition, some scholars at home and abroad believe that extending the retention time of drainage tube 41 is also a safe and feasible method for treating postoperative cerebrospinal fluid leakage. The pressure formed when the fascia layer accumulates reduces the tension of soft tissue and keeps the surgical incision dry. At the same time, with the continuous scar healing of muscles and fascia, the low-pressure area outside the dural sac gradually closes and shrinks, which is also conducive to the repair of the dural sac.

[0043] See also Figure 7 , Figure 8 and Fig. 9 In this regard, in this embodiment, from the completion of modeling until the drainage tube 41 is pulled out, considering that the experimental animal may pull out the drainage tube 41 spontaneously, the nursing clothes 50 are always worn and the drainage bag 42 is fixed with the nursing clothes 50, and it can be observed that there is no bloody exudate around the incision when the drainage tube 41 is connected. Moreover, as time goes by, the color of the drainage gradually becomes clear. Therefore, by observing the incision and drainage volume, the drainage tube 41 was pulled out on the 5th day of modeling. After the drainage tube 41 was pulled out, the incisions and drainage tubes 41 of all experimental animals healed well.

[0044] Thus, through the above seven steps, the non-human primate spinal cord hemisection injury model is completed.

[0045] Furthermore, in this embodiment, the positioning guide needle 22 is a straight needle, and the cross-sections of the needle body and the needle tip of the positioning guide needle 22 are both crescent-shaped, and the inner sides of the needle body and the needle tip are connected to form the sliding groove 221.

[0046] Furthermore, in this embodiment, the dura mater retractor 10 comprises two retractor brackets 11 and two fixing rods 12. The two retractor brackets 11 are arranged opposite to each other. Each retractor bracket 11 is formed with a support frame column 111 and a bracket head 112 integrally connected to the top of the support frame column 111. The bracket head 112 is detachably installed with a hook module 13 for lifting the dura mater in the middle thereof and has a rod arm 101 extending outwardly on both sides thereof. The rod arm 101 is opened on the upper surface thereof. A through hole 102 passing through the front and back sides thereof and a first threaded locking hole 103 communicating with the through hole 102 are provided; each fixing rod 12 is correspondingly installed between the front and rear corresponding rod mounting arms 101 of the two hook brackets 11, and both ends thereof pass through the through hole 102; a first locking screw 104 is installed on each first threaded locking hole 103, so that the first locking screw 104 can pass through the through hole 102, press against the fixing rod 12 and lock it, so that the two fixing rods 12 connect and fix the two hook brackets 11.

[0047] Furthermore, in this embodiment, the bracket head 112 is provided with a groove 105 in the middle thereof for installing the hook module 13 ; the hook module 13 includes a hook seat 131 detachably installed in the groove 105 and a hook body 132 installed on the hook seat 131 .

[0048] Furthermore, in this embodiment, the hook seat 131 is provided with a hook groove 1311 penetrating through the front and back sides thereof in the middle section of its top surface, and the hook groove 1311 is provided with a second threaded lock hole in the middle section of its bottom surface; the hook body 132 is formed with a main arm end 1321 inserted into the hook groove 1311 and a hook arm section 1322 integrally connected to the main arm end 1321 for lifting the dura mater; the main arm end 1321 is provided with an adjustment hole penetrating through its top and bottom surfaces. The strip opening 106 is provided with a second locking screw 107 corresponding to the second threaded locking hole, so that the second locking screw 107 can be inserted into the second threaded locking hole to press and lock the main arm end 1321 to fix the hook body 132 on the hook seat 131, and the second locking screw 107 can be loosened to allow the adjusting strip opening 106 to move forward and backward along the second locking screw 107 to adjust the relative position of the hook arm section 1322 and the hook seat 131.

[0049] Furthermore, in this embodiment, the dura mater protective sheet 30 is in the shape of a hook, and is formed with an inner hook section 31 and an outer arm section 32 integrally connected to the inner hook section 31. The recessed portion of the inner hook section 31 is a medullary groove for dura mater incision, and the dura mater protective sheet 30 extends the inner hook section 31 into the incision.

[0050] Furthermore, in the present embodiment, the nursing garment 50 is formed with a bra 51 and an abdominal belt 52 connected to the bottom of the bra 51, so that the bra 51 is worn on the chest and back and the abdominal belt 52 is used to cover the abdomen and back; the abdominal belt 52 is formed with an abdominal section 521 for fitting the abdomen, a strap section 522 connected to one side of the abdominal section 521 for fitting one side of the waist, the back and the other side of the waist in sequence, and a tie strap section 523 connected to the other side of the abdominal section 521 for being detachably folded and connected to the tie strap section 522, and a first stacking structure 501 is provided between the tie strap section 522 and the tie strap section 523; the drainage bag 42 is arranged on the inner layer of the tie strap section 523.

[0051] Furthermore, in the present embodiment, the bra 51 is provided with a front chest section 511 in the middle thereof, two back sections 512 are provided opposite to each other on both sides thereof, and an arm section 513 is provided between each back section 512 and the front chest section 511; a sleeve opening 514 is provided on the arm section 513, and a second overlapping structure 502 is provided between the two back sections 512, so that when the bra 51 is worn on the chest and back, the front chest section 511 fits against the front chest, the arms are respectively passed through the sleeve openings 514, and the two back sections 512 are overlapped and connected at the back through the second overlapping structure 502.

[0052] Furthermore, the method of disposing the drainage bag 42 in the inner layer of the strap section 523 can be flexibly designed, including but not limited to bagging or suturing. In this embodiment, the strap section 523 is provided with an inner bag 503 capable of accommodating the drainage bag 42 in the inner layer at one end thereof adjacent to the abdominal section 521.

[0053] Furthermore, in this embodiment, the inner layer of the abdominal belt 52 is provided with a gauze pad 504 to reduce discomfort after the abdominal belt 52 covers the abdomen and back, and can be used in conjunction with iodine to achieve the effects of disinfection and anti-pollution.

[0054] Furthermore, in this embodiment, the outer fabrics of the bra 51 and the abdominal belt 52 are both cut-resistant fabrics, including but not limited to ultra-high molecular weight polyethylene carbon fiber or Kevlar woven fabrics, which are tough, prevent monkeys from biting them, and are not easily pulled upwards by monkeys.

[0055] In summary, the method for making a non-human primate spinal cord hemisection injury model of the present invention sequentially exposes the spinal cord and dura mater, cuts the dura mater with the assistance of a dura mater retractor 10, inserts the positioning guide needle 22 into the central axis of the spinal cord using a stereotaxic instrument 21 and a positioning guide needle 22 installed on the stereotaxic instrument 21, inserts a dura mater protection sheet 30, uses a microsurgical knife to cut the spinal cord tissue downward along the slide groove 221 of the positioning guide needle 22, sutures the dura mater watertightly, and uses a drainage tube 41 to retain drainage under the epidural fascia and wears a nursing garment 50, thereby achieving the making of a non-human primate spinal cord hemisection injury model, ensuring the consistency of modeling and the success rate of modeling, improving the accuracy of hemisection, avoiding damage to the dura mater during hemisection, and performing post-suturing care with the nursing garment 50, thereby promoting wound healing.

[0056] As long as it does not violate the creative ideas of the present invention, any combination of the various embodiments of the present invention shall be regarded as the contents disclosed by the present invention; within the technical concept of the present invention, any combination of various simple modifications of the technical scheme and different embodiments that does not violate the creative ideas of the present invention shall be within the protection scope of the present invention.

Claims

1. A method for preparing a non-human primate spinal cord hemisection injury model, characterized in that: The following steps are involved: The first step is to expose the spinal cord and dura mater. Specifically, the spinous processes of T9 to T11 are located on the surface of the body, a 5 cm incision is made in the posterior midline, and the layers are separated downwards to expose the T10 lamina. The T10 spinous process and ligament are removed in sequence, the T10 lamina is removed by drilling, and then the yellow ligament is removed using microsurgical instruments to expose the spinal cord. The second step is to cut the dura mater with the assistance of the dura mater retractor (10). Specifically, a vertical incision is selected when opening the dura mater so as to facilitate suturing the dura mater after modeling is completed, thereby minimizing the degree of cerebrospinal fluid leakage. In order to reduce the damage to the spinal cord tissue when cutting the dura mater, the dura mater retractor (10) is used to assist in this step. The dura mater retractor (10) pulls the dura mater to create a gap between the dura mater and the spinal cord tissue, thereby avoiding damage to the spinal cord during the process of cutting the dura mater. The third step is to insert the positioning guide needle (22) into the central axis of the spinal cord using a stereotaxic instrument (21) and a positioning guide needle (22) mounted on the stereotaxic instrument (21), wherein the positioning guide needle (22) has a slide groove (221) therein to limit the moving track of the microsurgical knife, and the insertion position of the guide needle (22) is accurately positioned by the stereotaxic instrument (21), thereby ensuring the accuracy of the spinal cord hemisection range, and then the positioning guide needle (22) is driven to descend by the stereotaxic instrument (21) to pierce the spinal cord; The fourth step is to insert a dura mater protective sheet (30). Before the hemisection operation, the dura mater protective sheet (30) is placed on the side. The dura mater protective sheet (30) is inserted between the dura mater and the spinal cord tissue. The insertion depth is such that the tip of the dura mater protective sheet (30) touches the positioning guide needle (22). Step 5: Use a microsurgical knife to cut the spinal cord tissue downward along the slide groove (221) of the positioning guide needle (22) until the tip of the blade cuts the dura mater protective sheet (30) below. At this time, the microsurgical knife is cut outward to ensure that the spinal cord tissue on one side is completely cut off; Step 6: After the spinal cord hemisection model is completed, the dura mater is sutured watertightly and a drainage tube (41) is used to place an epidural subfascial drainage to reduce the risk of complications caused by postoperative cerebrospinal fluid leakage; The seventh step is to complete the modeling until the drainage tube (41) is pulled out. Considering that the experimental animal may pull out the drainage tube (41) spontaneously, it is necessary to wear a nursing garment (50) and fix the drainage bag (42) through the nursing garment (50). Then, by observing the incision and drainage volume, the drainage tube (41) is pulled out in time.

2. The method for preparing a non-human primate spinal cord hemisection injury model according to claim 1, characterized in that: The positioning guide needle (22) is a straight needle, and the cross-sections of the needle body and the needle tip of the positioning guide needle (22) are both crescent-shaped, and the inner sides of the needle body and the needle tip are connected to form the sliding groove (221).

3. The method for preparing a non-human primate spinal cord hemisection injury model according to claim 1, characterized in that: The dura mater retractor (10) comprises two retractor brackets (11) and two fixing rods (12). The two retractor brackets (11) are arranged opposite to each other. Each retractor bracket (11) is formed with a support frame column (111) and a bracket head (112) integrally connected to the top of the support frame column (111). The bracket head (112) is detachably installed with a hook module (13) for lifting the dura mater in the middle thereof and has rod mounting arms (101) extending outwardly on both sides thereof. The rod mounting arms (101) are provided with a plurality of holes extending through the front and back sides thereof. A through opening (102) and a first threaded locking hole (103) connected to the through opening (102); each fixing rod (12) is correspondingly installed between the front and rear corresponding rod mounting arms (101) of the two hook brackets (11), and its two ends pass through the through opening (102); each first threaded locking hole (103) is equipped with a first locking screw (104), which is used for the first locking screw (104) to pass through the through opening (102) to press against the fixing rod (12) and lock it, so that the two hook brackets (11) are connected and fixed by the two fixing rods (12).

4. The method for preparing a non-human primate spinal cord hemisection injury model according to claim 3, characterized in that: The bracket head (112) is provided with a groove (105) in the middle thereof for installing the hook module (13); the hook module (13) comprises a hook seat (131) detachably installed in the groove (105) and a hook body (132) installed on the hook seat (131).

5. The method for preparing a non-human primate spinal cord hemisection injury model according to claim 4, characterized in that: The hook seat (131) is provided with a hook groove (1311) penetrating through the front and back sides thereof in the middle of its top surface, and the hook groove (1311) is provided with a second threaded lock hole in the middle of its bottom surface; the hook body (132) is formed with a main arm end (1321) inserted into the hook groove (1311) and a hook arm section (1322) integrally connected to the main arm end (1321) for lifting the dura mater; the main arm end (1321) is provided with a positioning strip opening (106) penetrating through its top and bottom surfaces, The adjusting strip (106) is provided with a second locking screw (107) corresponding to the second threaded locking hole, so that the second locking screw (107) can be inserted into the second threaded locking hole to press and lock the main arm end (1321) to fix the hook body (132) on the hook seat (131). The adjusting strip (106) can be moved forward and backward along the second locking screw (107) by loosening the second locking screw (107) to adjust the relative position of the hook arm section (1322) and the hook seat (131).

6. The method for preparing a non-human primate spinal cord hemisection injury model according to claim 1, characterized in that: The dura mater protective sheet (30) is in the shape of a hook, and is formed with an inner hook section (31) and an outer arm section (32) integrally connected to the inner hook section (31). The recessed portion of the inner hook section (31) is a medullary groove for dura mater incision, and the dura mater protective sheet (30) extends the inner hook section (31) into the incision.

7. The method for preparing a non-human primate spinal cord hemisection injury model according to claim 1, characterized in that: The nursing garment (50) is formed with a bra (51) and an abdominal belt (52) connected to the bottom of the bra (51), the bra (51) is worn on the chest and back, and the abdominal belt (52) covers the abdomen and back; the abdominal belt (52) is formed with an abdominal section (521) for fitting the abdomen, a belt section (522) connected to one side of the abdominal section (521) for fitting one side of the waist, the back and the other side of the waist in sequence, and a tie section (523) connected to the other side of the abdominal section (521) and capable of being detachably folded and connected to the belt section (522), and a first folding structure (501) is provided between the belt section (522) and the tie section (523); the drainage bag (42) is arranged on the inner layer of the tie section (523).

8. The method for preparing a non-human primate spinal cord hemisection injury model according to claim 7, characterized in that: The bra (51) is provided with a front chest section (511) in the middle thereof, two back sections (512) are provided opposite to each other on both sides thereof, and an arm section (513) is provided between each back section (512) and the front chest section (511); a sleeve opening (514) is provided on the arm section (513), and a second overlapping structure (502) is provided between the two back sections (512), so that when the bra (51) is worn on the chest and back, the front chest section (511) fits the front chest, the arms are respectively passed through the sleeve openings (514), and the two back sections (512) are overlapped and connected at the back through the second overlapping structure (502).

9. The method for preparing a non-human primate spinal cord hemisection injury model according to claim 7, characterized in that: The outer fabrics of the bra (51) and the belly band (52) are both cut-resistant fabrics, and the belly band (52) is provided with a gauze pad (504) on its inner layer.

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