Hair repairing composition as well as preparation method and application thereof

Abstract

The invention discloses a hair repairing composition as well as a preparation method and application thereof, and relates to the technical field of daily chemicals. The hair repairing composition provided by the invention comprises 0.1-0.3 part of a dendrobium officinale extract, 0.15-0.4 part of a tremella extract, 10-60 parts of galactose yeast-like bacterium fermentation product filtrate and 39.3-89.75 parts of bifidobacterium fermentation product filtrate, and a polysaccharide biological film is formed on the surface of hair by utilizing the synergistic effect of the four components, so that the loss of moisture in the epidermal layer of the hair can be prevented, and the hair repairing effect is improved. And moisture in air or a solvent can be absorbed, so that hair cuticles are closed, and meanwhile, active substances rich in the four components can further penetrate into the hair to nourish the hair. Therefore, the hair repairing composition provided by the invention can improve the hair glossiness, improve the hair frizzy condition and reduce the combing power of dry hair and wet hair.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemicals, in particular to a hair repairing composition and a preparation method and application thereof. Background Art

[0002] Hair is composed of two parts: hair root, hair follicle and hair shaft. The hair shaft is the hair strand, which includes three parts: cuticle, cortex and medulla. The cuticle is the outermost layer of the hair fiber. It is composed of overlapping flat cells and presents a unidirectional fish scale structure. These structures are called "hair scales". The hair scales in the cuticle play an important barrier protection role for the hair. Once the hair structure is damaged, the cuticle is the easiest and first part to be damaged. Studies have shown that the hair scales of healthy hair are arranged tightly and neatly, while the hair that has been permed once has some hair scales curled up and is in an "open" shape; the hair that has been permed twice or three times has most of the hair scales curled up and fallen off, indicating that the hair cuticle is severely damaged and the integrity of the hair scale structure has decreased.

[0003] There are many factors that cause hair structural damage. In addition to the perm mentioned above, internal and external factors such as pressure, air pollution, and microorganisms can also have a certain impact on the integrity of the hair structure. Hair structural damage can involve morphological changes on the hair surface (such as curling, split ends, etc.), changes in color and gloss, and even changes in hair combing properties. These changes are also caused by severe damage to the epidermis, and the unidirectional and tightly arranged hair scales "open" or fall off.

[0004] Damage to the hair structure can cause many problems for people, such as difficulty in combing hair, affecting image, etc. Therefore, providing a hair repair composition that can ensure the integrity of the hair scale structure and thereby improve the glossiness and combability of hair has become one of the problems to be solved urgently. Summary of the invention

[0005] In order to address the deficiencies of the prior art, the present invention provides a hair repairing composition, which utilizes the synergistic effect of four components, namely, a Dendrobium officinale extract, a Tremella fuciformis extract, a filtrate of a fermentation product of Galactomyces saccharomyces, and a filtrate of a fermentation product of Bifida Saccharomyces, to repair damaged hair scales and improve the glossiness and combing properties of the hair.

[0006] Another object of the present invention is to provide a method for preparing the hair repair composition.

[0007] Another object of the present invention is to provide a hair care product.

[0008] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0009] A hair repair composition, comprising the following components calculated by weight:

[0010] 0.1-0.3 parts of Dendrobium officinale extract, 0.15-0.4 parts of Tremella fuciformis extract, 10-60 parts of galactosaccharomyces fermentation product filtrate, and 39.3-89.75 parts of bifid yeast fermentation product filtrate.

[0011] In the hair repair composition provided by the present invention, the Dendrobium officinale extract is rich in Dendrobium officinale polysaccharide and flavonoids, wherein the Dendrobium officinale polysaccharide has a good moisturizing effect, and the flavonoids have a good antioxidant effect. When the Dendrobium officinale extract is applied to the surface of hair, the frizzy state of the hair can be improved, the combing property of the hair can be improved, and the hair scales can be repaired. Tremella fuciformis Berk extract is rich in active substances such as Tremella fuciformis polysaccharide, which is rich in a large number of polar groups such as hydroxyl and carboxyl groups, and can bind a large amount of water. The final product shows a good effect of repairing hair. The filtrate of the fermentation product of the galactosyeast-like bacteria is obtained by fermentation of Trichosporon, is rich in vitamins, minerals, amino acids, organic acids, enzymes, and yeast peptides, and has a good moisturizing effect. The bifid yeast fermentation product filtrate is a fermentation product filtrate obtained by post-fermentation of bifidobacteria, which is rich in vitamins, minerals, amino acids, polysaccharides, polypeptides and other active small molecules, and therefore exhibits certain moisturizing, anti-oxidation, and anti-aging effects, and can promote the repair of damaged DNA. These advantages allow the bifid yeast fermentation product filtrate to be applied to skin care products to improve skin quality. Based on the above functions of the bifid yeast fermentation product filtrate, the present invention adds the bifid yeast fermentation product filtrate to the hair repair composition so that the final product exhibits better hair repair effects.

[0012] After the above four components are mixed to form the hair repair composition of the present invention, the Dendrobium officinale polysaccharide rich in the Dendrobium officinale extract and the Tremella fuciformis polysaccharide rich in the Tremella fuciformis extract can interact through hydrogen bonds and electrostatics to form a cross-network structure, forming a biopolysaccharide film on the surface of the hair, which not only plays a sealing role to prevent the loss of moisture in the epidermis of the hair, but also can absorb moisture in the air or solvent through the hydroxyl and carboxyl groups of the polysaccharide, so that the hair scales are closed, the hair frizziness is reduced, and the hair is flexible and smooth. At the same time, the four components in the composition of the present invention are rich in active substances such as amino acids, polypeptides, and vitamins. These active substances can penetrate the epidermis and penetrate into the hair cortex, effectively supplement the nutrients required for damaged hair, and nourish the hair from the inside. In addition, the Dendrobium officinale extract is rich in flavonoids, which can reduce oxidative damage to hair, reduce the breakage of disulfide bonds, the degradation of amino acids and the oxidation of lipids in the epidermis, and reduce the problems of roughness, decreased toughness, and reduced mechanical properties of hair. Overall, the composition formed by the present invention, which is formed by combining Dendrobium officinale extract, Tremella fuciformis extract, galactosylceras fermentation product filtrate and bifid yeast fermentation product filtrate, can synergistically enhance the effects and repair hair from the inside out.

[0013] Preferably, the Dendrobium officinale extract is prepared by enzymatically extracting Dendrobium officinale using an immobilized enzyme;

[0014] The tremella extract is prepared by fermenting and extracting tremella with Candida utilis.

[0015] The tremella extract in the composition provided by the present invention is preferably prepared by fermentation extraction with Candida utilis, and the tremella polysaccharide in the obtained extract has an ultra-low molecular weight; the tremella polysaccharide in the tremella extract prepared by other methods has a higher molecular weight and a reduced hair repairing effect; at the same time, the Dendrobium officinale extract used in the present invention is also preferably obtained by enzymolysis using an immobilized enzyme, so that the Dendrobium officinale polysaccharide and flavonoids in the obtained extract have lower molecular weights.

[0016] Preferably, the hair repair composition comprises the following components calculated by weight:

[0017] 0.13-0.16 parts of Dendrobium officinale extract, 0.25-0.3 parts of Tremella fuciformis extract, 28-43 parts of galactosaccharomyces fermentation product filtrate, and 56-72 parts of bifid yeast fermentation product filtrate.

[0018] The inventors have experimentally confirmed that when the proportions of the components are within the above ranges, the resulting composition has a more excellent hair repairing effect.

[0019] Preferably, the immobilized enzyme comprises an immobilized enzyme carrier and an enzyme loaded on the immobilized enzyme carrier, and the enzyme comprises at least one of Aspergillus niger acid β-mannanase, cellulase, product enzyme of Bacillus nubiquitus and neutral protease.

[0020] Preferably, the method for preparing the Dendrobium officinale extract comprises the following steps:

[0021] The powder of Dendrobium officinale stem is dispersed in water to obtain a solution, and the solution is heated at 60-80° C. to perform water extraction; after the water extraction, an immobilized enzyme is added to perform enzymolysis on the Dendrobium officinale to obtain the Dendrobium officinale extract.

[0022] More preferably, the mass ratio of the Dendrobium officinale stem powder to water is 1:(20-40).

[0023] The inventors have experimentally confirmed that when the mass ratio of Dendrobium officinale stem powder to water is within the above range, the resulting composition has a more excellent hair repairing effect.

[0024] In a specific embodiment of the present invention, the pH of the solution in the water extraction is 6.0-6.8.

[0025] In a specific embodiment of the present invention, the heating time in the water extraction is 20-40 minutes.

[0026] In a specific embodiment of the present invention, the preparation method of the immobilized enzyme is carried out according to Chinese Patent CN117106764A.

[0027] In a specific embodiment of the present invention, the added amount of the immobilized enzyme is 3-8% of the mass of the Dendrobium officinale stem powder.

[0028] In a specific embodiment of the present invention, the temperature of the enzymatic hydrolysis is 60-70°C, and the time of the enzymatic hydrolysis is 80-160 min.

[0029] In a specific embodiment of the present invention, the enzymatic hydrolysis further includes the steps of filtration and sterilization.

[0030] In a specific embodiment of the present invention, the sterilization temperature is 80-95° C., and the sterilization time is 15-30 min.

[0031] Preferably, the Tremella fuciformis extract is prepared by using a bacterial concentration of 10 7 -10 9 The method is prepared by fermenting and extracting Tremella fuciformis seed liquid with CFU / mL of Candida utilis seed liquid, and the mass ratio of the Candida utilis seed liquid to Tremella fuciformis is (2-5): (0.5-1.8).

[0032] In a specific embodiment of the present invention, the method of fermenting and extracting Tremella fuciformis with Candida utilis comprises: inoculating the seed liquid of Candida utilis into Tremella fuciformis fermentation medium for fermentation, thereby obtaining the Tremella fuciformis extract.

[0033] More specifically, the fermentation temperature is 28-33° C., and the fermentation time is 40-60 hours.

[0034] More specifically, the fermentation is carried out under stirring conditions at a rotation speed of 100-200 rpm.

[0035] More specifically, the Tremella culture medium comprises 0.5-1.8% Tremella, 1-4% carbon source, 0.3-1.2% nitrogen source and 0.35-0.8% inorganic salt, and the balance is water.

[0036] More specifically, the Tremella includes at least one of Tongjiang Tremella, Gutian Tremella, Guizhou Tremella, and Yunnan Tremella; the carbon source includes at least one of fructose syrup, dextrin, soybean starch, potato starch, and molasses; the nitrogen source includes at least one of soy peptone, yeast extract, cottonseed powder, and tryptone; and the inorganic salt includes sulfate and / or phosphate.

[0037] More specifically, the fermentation and extraction further include sterilization and filtration steps.

[0038] Preferably, the sterilization temperature is 80-95°C, and the sterilization time is 15-30 minutes.

[0039] The present invention also protects a method for preparing a hair repair composition, comprising the following steps:

[0040] The hair repairing composition can be obtained by mixing the Dendrobium officinale extract, Tremella fuciformis extract, the filtrate of the fermentation product of Galacto-saccharomyces cerevisiae and the filtrate of the fermentation product of Bifidobacterium.

[0041] The present invention also protects a hair care product comprising the above hair repairing composition.

[0042] The hair care product of the present invention is any preparation conventionally prepared in the field of hair products, and can be formulated into at least one of, for example, a solution, a suspension, an emulsion, a paste, a gel, a cream, water, a powder, a soap, a detergent containing a surfactant, an oil, a powder cream, and a spray.

[0043] In a specific embodiment of the present invention, the hair care product of the present invention includes at least one of hair conditioner, hair nourishing cream, hair massage cream, hair essence, hair cleansing cream, hair cleansing foam, hair cleansing water, hair moisturizing cream, shampoo, and conditioner.

[0044] In a specific embodiment of the present invention, the hair repair composition accounts for 0.1-100% by weight in the hair care product.

[0045] Compared with the prior art, the present invention has the following beneficial effects:

[0046] The hair repair composition provided by the present invention can form a biopolysaccharide film on the surface of hair, has good moisture absorption and water retention effects, and can repair the hair scale structure. When the composition provided by the present invention is used to repair hair, the glossiness of the hair can be increased by more than 3%, and the dry and wet hair combing work can be reduced by 3.79% and 29.57% respectively, which can greatly improve the smoothness of the hair. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 This is a scanning electron micrograph of the hair bundles in the blank control group during the hair cuticle repair test.

[0048] Figure 2 This is a scanning electron microscope image of the hair bundle of the test group in Example 1 during the hair cuticle repair test.

[0049] Figure 3 This is a scanning electron microscope image of the hair bundle of the test group in Example 2 during the hair cuticle repair test.

[0050] Figure 4 This is a scanning electron microscope image of the hair bundle of the test group of Example 3 during the hair cuticle repair test.

[0051] Figure 5 This is a scanning electron microscope image of the hair bundle of the test group of Example 4 during the hair cuticle repair test. DETAILED DESCRIPTION

[0052] The present invention is further described below in conjunction with specific embodiments, but the embodiments do not limit the present invention in any form. Unless otherwise specified, the percentages and inoculum amounts mentioned in the present invention are all mass percentages. Unless otherwise specified, the raw material reagents used in the embodiments of the present invention are conventionally purchased raw material reagents.

[0053] The raw material information used in each embodiment and comparative example is as follows:

[0054] Galactomyces galactosidase fermentation product filtrate: purchased from Guangzhou Giant Microbiology Technology Co., Ltd., the product number is GalaFF.

[0055] Bifid yeast fermentation product filtrate: purchased from Perfect (Guangdong) Daily Necessities Co., Ltd., material code is C00817.

[0056] Dendrobium officinale extract: prepared by the following method:

[0057] The powder of Dendrobium officinale stem is dispersed in 30 times the mass of water to obtain a solution, the pH of the solution is adjusted to 6.5 by 1wt% hydrochloric acid solution, and the solution is heated at 70°C for 30 minutes to obtain a suspension; 5% of the mass of the Dendrobium officinale stem powder is added to the suspension, and the suspension is enzymolyzed at 65°C for 120 minutes, filtered through a diatom filter, and the obtained filtrate is sterilized at 90°C for 20 minutes to obtain the Dendrobium officinale extract; the preparation method of the immobilized enzyme is carried out according to Example 1 in Chinese patent CN117106764A.

[0058] Tremella fuciformis extract: prepared by the following method:

[0059] The bacterial concentration was 10 8 The CFU / mL Candida utilis seed liquid is inoculated into a Tremella fermentation medium at an inoculation rate of 3%, and the fermentation is carried out for 50 hours at 30°C and a rotation speed of 150 rpm in a fermentation shake flask to prepare a crude fermentation liquid, which is sterilized by keeping warm at 90°C for 20 minutes, and filtered through a microfiltration membrane after cooling to obtain a Tremella extract; the formula of the Tremella fermentation medium is: 1.2% of Tongjiang Tremella powder, 2% of fructose syrup, 0.8% of soy peptone, 0.3% of magnesium sulfate, 0.15% of potassium dihydrogen phosphate, 0.1% of potassium dihydrogen phosphate and the balance of water.

[0060] Candida utilis: Candida utilis GDMCC2.148 was purchased from Guangdong Provincial Microbiological Culture Collection Center.

[0061] The ganoderma lucidum extract, brown algae extract, yeast fermentation product extract and lactic acid bacteria fermentation extract used in the present invention are all purchased from Dongguan Juwei New Material Technology Co., Ltd.

[0062] Examples 1 to 6 and Comparative Examples 1 to 11

[0063] This embodiment and comparative example provide a series of hair repair compositions with different mass fractions of components, and the specific mass fractions of components are shown in the following Table 1:

[0064] Table 1. Mass fractions of each component in Examples 1 to 6 and Comparative Examples 1 to 11

[0065]

[0066]

[0067] Comparative Example 12

[0068] A hair repair composition, which differs from Example 2 only in that:

[0069] In the preparation method of the Dendrobium officinale extract, 3% of cellulase by weight of the Dendrobium officinale stem powder and 2% of pectinase by weight of the Dendrobium officinale stem powder are used for enzymolysis.

[0070] Comparative Example 13

[0071] A hair repair composition, which differs from Example 2 only in that:

[0072] The Dendrobium officinale extract is prepared by fermenting and extracting Dendrobium officinale with Candida utilis. The specific preparation method comprises the following steps:

[0073] The bacterial concentration was 10 8 CFU / mL of Candida utilis seed solution was inoculated into the Dendrobium officinale fermentation medium at an inoculum rate of 3%, and fermented for 50 hours at 30°C and a fermentation shake flask at a rotation speed of 150 rpm to prepare a crude fermentation liquid, which was kept at 90°C for 20 minutes, cooled, and filtered through a microfiltration membrane to obtain a Dendrobium officinale extract;

[0074] The formula of the Dendrobium officinale fermentation medium is: 1.2% of Dendrobium officinale stem powder, 2% of fructose syrup, 0.8% of soy peptone, 0.3% of magnesium sulfate, 0.15% of potassium dihydrogen phosphate, 0.1% of potassium hydrogen phosphate and the balance of water.

[0075] Comparative Example 14

[0076] A hair repair composition, which differs from Example 2 only in that:

[0077] Tremella extract was prepared by fermenting Tremella with Saccharomyces cerevisiae. The specific fermentation and extraction method was consistent with the method of fermentation and extraction with Candida utilis in Example 2, except that the seed solution of Candida utilis was replaced with the seed solution of Saccharomyces cerevisiae (the concentration of the bacteria was 10 8 CFU / mL).

[0078] The brewer's yeast described in this comparative example is brewer's yeast BNCC336054, which was purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0079] Comparative Example 15

[0080] A hair repair composition, which differs from Example 2 only in that:

[0081] The preparation of Tremella fuciformis extract includes the following steps:

[0082] The Tongjiang Tremella powder is dispersed in 30 times the mass of water to obtain a solution, the pH of the solution is adjusted to 6.5 by a 1wt% hydrochloric acid solution, and the solution is heated at 70°C for 30 minutes to obtain a suspension; an immobilized enzyme of 5% by mass of the Tongjiang Tremella powder is added to the suspension, and after enzymolysis at 65°C for 120 minutes, the suspension is filtered through a diatom filter, and the obtained filtrate is sterilized by keeping warm at 90°C for 20 minutes to obtain a Tremella extract; the preparation method of the immobilized enzyme is carried out according to Example 1 in Chinese patent CN117106764A.

[0083] Comparative Example 16

[0084] A hair repair composition, which differs from Example 2 only in that:

[0085] The Dendrobium officinale extract was replaced with Ganoderma lucidum extract of equal quality.

[0086] Comparative Example 17

[0087] A hair repair composition, which differs from Example 2 only in that:

[0088] Replace Tremella fuciformis extract with an equal mass of brown algae extract.

[0089] Comparative Example 18

[0090] A hair repair composition, which differs from Example 2 only in that:

[0091] The galactosaccharomyces fermentation filtrate was replaced with an equal mass of yeast fermentation extract.

[0092] Comparative Example 19

[0093] A hair repair composition, which differs from Example 2 only in that:

[0094] The filtrate of the bifid yeast fermentation product was replaced with an equal mass of the lactic acid bacteria fermentation extract.

[0095] Performance Testing

[0096] Next, the compositions obtained in Examples 1 to 6 and Comparative Examples 1 to 19 were tested to explore their stability, hair repairing effects and other properties.

[0097] 1. Stability test

[0098] Test samples: hair repair compositions of Examples 1 to 6 and Comparative Examples 1 to 19.

[0099] Test steps: Take 15 mL of the test sample and put it in a colorless and transparent PE sample bottle, seal the sample and place it at 25°C for 3 weeks. Test the conductivity, pH and viscosity of the sample in the 0th week and the 3rd week respectively.

[0100] Test equipment:

[0101] Conductivity: Conductivity meter, Leici DDBJ-350.

[0102] pH: pH meter, SevenCompact S210-S.

[0103] Viscosity: Rotational viscometer, IKA ROTAVISC ME-VI S000.

[0104] The test results are shown in Table 2 below.

[0105] Table 2. Stability data of the compositions obtained in the examples and comparative examples

[0106]

[0107]

[0108]

[0109] The test results show that the hair repair compositions of Examples 1-6 of the present invention have good stability. After being placed at room temperature for 3 weeks, the changes in conductivity, pH and viscosity are not large, so they have application prospects for large-scale commercial production. However, after being placed at room temperature for 3 weeks, the conductivity, pH and viscosity of the hair repair compositions of some comparative examples have large changes, indicating that their stability is poor. Among them, the hair compositions of Comparative Examples 1 and 2 lack one of the Dendrobium officinale extract and Tremella fuciformis extract, respectively, and Comparative Examples 16 and 17 replace the Dendrobium officinale extract and Tremella fuciformis extract with similar plant extracts, respectively. The conductivity, pH and viscosity of the hair compositions finally obtained have large changes, indicating that the Dendrobium officinale extract and Tremella fuciformis extract play an important role in maintaining the stability of the system.

[0110] 2. Hair gloss test

[0111] Test samples: hair repair compositions of Examples 1 to 6 and Comparative Examples 1 to 19.

[0112] Test steps:

[0113] (1) Hair bundle pretreatment: Prepare 75 damaged hair bundles and randomly divide them into 25 groups, with 3 hair bundles in each group. Before testing, clean the hair bundles with a 3% SDS aqueous solution and dry them at room temperature for later use.

[0114] (2) Sample use: Gently and evenly apply 1.5 mL of the test sample to the upper, middle and lower parts of the hair bundle. After leaving it to dry, wash the hair bundle with deionized water and let it dry before testing.

[0115] (3) Glossiness test: After the hair bundle is treated and the sample is used, the hair bundle is fixed with a test clip and the glossiness of the hair bundle surface is tested using a skin glossiness probe. The relevant data is recorded and the glossiness change rate of the hair before and after the use of the test sample is calculated. Each sample is tested three times in parallel and the test results are averaged. The test instrument is a skin glossiness probe Glossymeter GL200.

[0116] The glossiness change rate of the hair (%) = (glossiness of the hair bundle after using the test sample - glossiness of the hair bundle after the pre-treatment of the hair bundle) / glossiness of the hair bundle after the pre-treatment of the hair bundle × 100%.

[0117] When the glossiness change rate of the hair is a positive value, it means that the test sample has the effect of brightening the hair.

[0118] The test results are shown in Table 3 below:

[0119] Table 3. Glossiness test data of examples and comparative examples

[0120]

[0121]

[0122] The test results show that the hair repair composition of the embodiment of the present invention has a good effect of brightening the gloss of hair. After using the hair repair composition of the embodiment of the present invention, the gloss of the hair is brightened by more than 3%, up to 34.59%. Among them, the gloss change rate of Example 2 is much higher than that of Comparative Examples 1 to 19, indicating that the Dendrobium officinale extract and Tremella fuciformis extract obtained by the extraction method of the present invention can have a synergistic effect with the filtrate of the fermentation product of galactosaccharomyces galactosidase and the filtrate of the fermentation product of bifid yeast. No matter which component is missing (Comparative Examples 1 to 5), the performance of the composition in improving the gloss of hair will decrease; using other extraction methods to prepare the two plant extracts of the present invention (Comparative Examples 12 to 15) will also lead to a decrease in synergistic effect; replacing any of the four components with other components with similar functions (Comparative Examples 16 to 19) will also lead to a loss of synergistic effect. At the same time, according to the data comparison between the embodiment and Comparative Examples 6 to 11, it can be seen that the mass proportion of each component in the composition provided by the present invention should be within an appropriate range to ensure the maximum synergistic effect.

[0123] 3. Dry hair combing effect test

[0124] Test samples: hair repair compositions of Examples 1 to 6 and Comparative Examples 1 to 19.

[0125] Test steps:

[0126] (1) Hair bundle pretreatment: 78 damaged hair bundles were prepared and randomly divided into 26 groups, with 3 hair bundles in each group. Before testing, the hair bundles were cleaned with a 3% SDS aqueous solution and dried at room temperature for later use.

[0127] (2) Sample use: Gently and evenly apply 1.5 mL of the test sample to the upper, middle and lower parts of the hair bundle, rinse with tap water while smoothing the hair, rinse thoroughly, and dry at room temperature before testing. The blank control group does not apply the sample, and the hair bundle is washed with tap water together with the sample group, and then dried before testing.

[0128] (3) Dry hair combing test: After the hair bundle is treated and the sample is used, the hair bundle is fixed with a test clip and the combing test is performed. The test is repeated 5 times. Three hair bundles are used for each test sample for parallel testing. The relevant data are recorded and the combing power change rate of the hair bundle before and after the use of the test sample is calculated. The test results are averaged. The test instrument is the hair tester MTT175 (Tress Volume Accessory).

[0129] Change rate of dry hair combing work (%) = (combing work of the hair bundle after using the test sample - combing work of the hair bundle after the pre-treatment of the hair bundle) / combing work of the hair bundle after the pre-treatment of the hair bundle × 100%.

[0130] The smaller the combing work of the hair bundle, the easier it is to comb the hair bundle. That is, when the change rate of the dry hair combing work is a negative value, it means that the test sample has the effect of smoothing the hair.

[0131] 4. Wet hair combing effect test

[0132] Test samples: hair repair compositions of Examples 1 to 6 and Comparative Examples 1 to 19.

[0133] Test steps:

[0134] (1) Hair bundle pretreatment: 78 damaged hair bundles were prepared and randomly divided into 26 groups, with 3 hair bundles in each group. Before testing, the hair bundles were cleaned with a 3% SDS aqueous solution and dried at room temperature for later use.

[0135] (2) Sample application: Apply 1.5 mL of the test sample gently and evenly on the upper, middle and lower sections of the hair bundle, rinse with tap water while smoothing the hair, and rinse thoroughly. The blank control group does not apply the sample, but washes the hair bundle with tap water together with the sample group, and then waits for testing after drying.

[0136] (3) Wet hair combing performance test: After the hair bundle is treated before use, soak the hair bundle in clean water, comb it, gently knead it, and wet it. Then, clamp it on the test clip for wet hair combing performance test. Repeat the test 5 times, and record the wet hair combing performance before use. After using the sample, rinse it with clean water, then clamp it on the test clip for wet hair combing performance test. Repeat the test 5 times, and record the wet hair combing performance after use. Each test sample is tested in parallel with 3 hair bundles. Each group records the relevant data and calculates the combing performance change rate of the hair bundle before and after using the test sample. The test results are averaged. The test instrument is the hair tester MTT175 (Tress Volume Accessory accessory).

[0137] Change rate of wet hair combing work of hair (%) = (combing work of hair bundle after using test sample - combing work of hair bundle after pre-treatment of hair bundle) / combing work of hair bundle after pre-treatment of hair bundle × 100%.

[0138] The smaller the combing work of the hair bundle, the easier it is to comb the hair bundle. That is, when the change rate of the wet combing work of the hair is a negative value, it means that the test sample has the effect of smoothing the hair.

[0139] The results of the above dry and wet hair combing effect tests are shown in Table 4 below:

[0140] Table 4. Test data of combability of dry and wet hair of Examples and Comparative Examples

[0141]

[0142]

[0143] The smoothness of hair is one of the commonly used parameters for evaluating the effects of hair products. When hair is damaged by physical or chemical treatment, the loss of liposomes and the loss or curling of hair scales will cause the hair surface to become rough, thereby increasing the friction between hair strands and reducing smoothness. The smoothness of hair can be quantified by the combing force or combing work recorded when the comb passes through the hair when the hair is wet or dry, which is commonly known as dry combing and wet combing.

[0144] The test results show that, compared with the blank group, after using the hair repair composition of the embodiments of the present invention, the hair is repaired, which is further manifested in that the combing work change rate of the hair is lower than that of the blank control group. The dry hair combing work change rate and the wet hair combing work change rate of all the embodiments are negative values, indicating that after using the samples of Examples 1-6, the hair becomes easier to comb.

[0145] 5. Hair scale repair test

[0146] Test sample: Hair repair composition of Examples 1-4.

[0147] Test steps: Prepare 5 damaged hair bundles. Before testing, clean the hair bundles with 3% SDS aqueous solution, dry them at room temperature and set aside. Soak the hair bundles in the test sample and deionized water (blank control group) for 10 minutes, take them out after soaking, dry them at room temperature, and then take pictures with a scanning electron microscope. The test results are as follows: Figures 1 to 5 As shown. Figure 1 As shown in the figure, when hair is damaged, the hair scales on its surface may become warped, broken, wrinkled, etc. After using the hair repair composition of the embodiment of the present invention, the warping of the hair scales is improved and the hair scales are closed, indicating that the hair repair composition of the present invention has the function of repairing the structural integrity of the hair scales. Figure 2-5 It can be seen that the hair repairing composition of Example 2 has the best effect in repairing hair cuticles.

[0148] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A hair repair composition, characterized in that: It includes the following components calculated by mass: 0.1-0.3 parts of Dendrobium officinale extract, 0.15-0.4 parts of Tremella fuciformis extract, 10-60 parts of galactosaccharomyces fermentation product filtrate, and 39.3-89.75 parts of bifid yeast fermentation product filtrate.

2. The hair repair composition according to claim 1, characterized in that: The Dendrobium officinale extract is prepared by enzymatically extracting Dendrobium officinale with an immobilized enzyme; and the Tremella fuciformis extract is prepared by fermenting Tremella fuciformis with Candida utilis.

3. The hair repair composition according to claim 1, characterized in that: It includes the following components calculated by mass: 0.13-0.16 parts of Dendrobium officinale extract, 0.25-0.3 parts of Tremella fuciformis extract, 28-43 parts of galactosaccharomyces fermentation product filtrate, and 56-72 parts of bifid yeast fermentation product filtrate.

4. The hair repair composition according to claim 2, characterized in that: The immobilized enzyme comprises an immobilized enzyme carrier and an enzyme loaded on the immobilized enzyme carrier, and the enzyme comprises at least one of Aspergillus niger acid β-mannanase, cellulase, product enzyme of anaerobic Bacillus nubifolius and neutral protease.

5. The hair repair composition according to claim 2, characterized in that: The preparation method of the Dendrobium officinale extract comprises the following steps: The Dendrobium officinale stem powder is dispersed in water to obtain a solution, and the solution is heated at 60-80° C. to perform water extraction; after the water extraction, the immobilized enzyme is added to perform enzymolysis on the Dendrobium officinale to obtain the Dendrobium officinale extract.

6. The hair repair composition according to claim 5, characterized in that: The mass ratio of the Dendrobium officinale stem powder to water is 1:(20-40).

7. The hair repair composition according to claim 6, characterized in that: The added amount of the immobilized enzyme is 3-8% of the mass of the Dendrobium officinale stem powder.

8. The hair repair composition according to claim 2, characterized in that: The Tremella fuciformis extract is prepared by using a bacterial concentration of 10 7 -10 9 The method is prepared by fermenting and extracting Tremella fuciformis seed liquid with CFU / mL of Candida utilis seed liquid, and the mass ratio of the Candida utilis seed liquid to Tremella fuciformis is (2-5):(0.5-1.8).

9. A method for preparing the hair repair composition according to any one of claims 1 to 8, comprising the following steps: The hair repairing composition can be obtained by mixing the Dendrobium officinale extract, Tremella fuciformis extract, the filtrate of the fermentation product of galactosaccharomyces cerevisiae and the filtrate of the fermentation product of bifid yeast.

10. A hair care product, characterized in that: The hair repairing composition comprises the hair repairing composition according to any one of claims 1 to 8.

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