Glucose clamp test device based on negative feedback model

Abstract

The invention relates to the technical field of glucose clamp tests, in particular to a glucose clamp test device based on a negative feedback model, which comprises a table body, a test box is fixedly mounted on the front side of the upper surface of the table body, box doors are rotatably mounted on two sides of the front end of the test box respectively, and a fixed clamping plate is connected to the inner side of the test box through an XY-axis moving mechanism; an upper connecting plate and a lower connecting plate are fixedly connected to the upper end and the lower end of the fixed clamping plate respectively, a movable clamping plate is connected to one side of the fixed clamping plate through an inserting mechanism, a sliding opening is formed in the upper surface of the upper connecting plate in a penetrating mode, and two sliding blocks are slidably inserted into the inner side of the sliding opening through elastic meshing mechanisms respectively; the device has the function of positioning the white mouse, the white mouse does not need to be subjected to anesthesia treatment or binding treatment in a test, and operation is convenient; a punctured needle head can be firmly positioned, so that the problems of separation and looseness are prevented; and the problem that the rear legs of the white mouse touch the connecting pipe can be effectively prevented, so that the test is blocked.

Description

Technical Field

[0001] The invention relates to the technical field of glucose clamp test, and in particular to a device for performing glucose clamp test based on a negative feedback model. Background Art

[0002] Glucose clamp test is an important method in the development of insulin drugs. According to different research purposes, glucose clamp technology is divided into high glucose clamp, positive glucose clamp and low glucose clamp. Among them, glucose clamp test is currently recognized as the best method to evaluate the PK / PD characteristics of insulin and is widely used in the clinical development of insulin drugs.

[0003] In the positive glucose clamp test, the hypoglycemic effect caused by the increase in blood insulin concentration (through exogenous administration of test or non-test insulin preparations) is antagonized by the continuously adjusted intravenous glucose infusion, so that endogenous insulin secretion is effectively inhibited, and the blood glucose level is "clamped" within a predetermined target range. When the "clamp" test system is stable, after the test insulin drug is administered, its PK characteristics can be described by detecting the changes in the test drug concentration in plasma over time; on the other hand, the time-action curve of the test drug can be drawn by the changes in the glucose infusion rate required to keep the blood glucose concentration stable within the target range in the clamp test, and its PD characteristics can be described.

[0004] Traditional test operation management is complex and requires high skills of medical staff, especially in the process of adjusting glucose injection. The current method is to adjust the next glucose injection amount based on the doctor's experience and judgment. How to accurately control the glucose injection rate to achieve the best measurement effect is a technical problem. Therefore, it is more common to use a negative feedback model to adjust the glucose injection amount in the glucose clamp test. This method simplifies the test process and constructs a mathematical model to optimize the glucose injection amount to adjust the accuracy of the test. After the test is completed, the model can be further optimized based on the measured data to make the next test closer to the real efficacy data. However, in the white mouse test, it is still necessary to intermittently perform needle injections and needle blood draws, which is quite inconvenient. At the same time, in order to facilitate the operation, the white mouse needs to be paralyzed or tied before the experiment, which is also cumbersome and inconvenient. Summary of the invention

[0005] The purpose of the present invention is to solve the shortcomings existing in the background technology and to propose a glucose clamp test device based on a negative feedback model.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is: a glucose clamp test device based on a negative feedback model, including a platform, a test box is fixedly installed at the front edge of the upper surface of the platform, box doors are rotatably installed on both sides of the front end of the test box, the inner side of the test box is connected with a fixed clamp plate through an XY axis moving mechanism, the upper and lower ends of the fixed clamp plate are respectively fixedly connected with an upper connecting plate and a lower connecting plate, one side of the fixed clamp plate is connected with a movable clamp plate through a plug-in mechanism, a sliding opening is penetrated through the upper surface of the upper connecting plate, two sliders are slidably inserted on the inner side of the sliding opening through an elastic meshing mechanism, the lower end of the slider is fixedly connected with a pressure plate, and presser feet are respectively fixedly connected on both sides of the lower surface of the pressure plate, needles are respectively arranged under each of the two presser feet, and needle handles are fixedly arranged on the outer surface of the needles.

[0007] Preferably, the XY-axis moving mechanism includes guide rails fixedly connected to the front and rear edges of the upper surface of the test box, guide blocks are slidably mounted on the inner sides of the guide rails, two guide rods are fixedly connected between the front and rear guide blocks, the outer surface of the fixed clamp is fixedly connected to a connecting rod, two sleeves are fixedly embedded on the upper ends of the connecting rods, and the sleeves are slidably mounted on the outer surfaces of the connecting rods.

[0008] Preferably, the upper end of the needle is fixedly connected with a connecting tube, the upper surface of the connecting rod is fixedly connected with a wire-passing plate extending toward the rear, and the connecting tube passes through the outer surface of the wire-passing plate.

[0009] Preferably, one end of the connecting tubes is connected to an injection mechanism, and the other end of the connecting tube is connected to a detection mechanism, the injection mechanism and the detection mechanism are respectively fixedly mounted on the upper surface of the platform, and a host module is fixedly mounted on the upper surface of the platform.

[0010] Preferably, the elastic engagement mechanism includes a lower guide plate fixedly connected to the front and rear surfaces of the slider, respectively, the upper surface of the lower guide plate slides against the lower surface of the upper connecting plate, the outer surface of the slider is provided with a lifting plate through an elastic mechanism sliding sleeve, the lower surface of the lifting plate is fixedly connected with a plurality of limiting teeth, the upper surface of the upper connecting plate is provided with a limiting tooth groove, and the limiting teeth are inserted into the inner side of the limiting tooth groove.

[0011] Preferably, the elastic mechanism includes a top connecting plate fixedly connected to the upper end of the slider, a spring is fixedly connected to the lower surface of the top connecting plate, the lower end of the spring is fixedly connected to the upper surface of the lifting plate, a plurality of lifting columns are fixedly connected to the upper surface of the lifting plate, the lifting columns slide through the lower surface of the top connecting plate, and the upper ends of the plurality of lifting columns are commonly fixedly connected to a pulling plate.

[0012] Preferably, a splicing plate is fixedly connected at the lower end of the movable clamping plate, a socket is penetrated through the edge of the lower surface of the splicing plate, a threaded column is fixedly connected to the lower surface of the lower connecting plate, the threaded column slides through the inner side of the socket, a positioning sleeve is provided on the threaded sleeve at the lower end of the threaded column, and the upper surface of the positioning sleeve contacts the lower surface of the splicing plate.

[0013] Preferably, the inner wall of the fixed clamping plate and the upper surface of the lower connecting plate are fixedly connected with a first protective pad, and the inner wall of the movable clamping plate is fixedly connected with a second protective pad.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. The present invention has the function of locating mice, and there is no need to anesthetize or tie up the mice during the experiment, which is convenient to operate;

[0016] 2. The present invention can firmly position the inserted needle to prevent the problem of detachment and loosening;

[0017] 3. The present invention can effectively prevent the problem of the hind legs of the white mouse touching the connecting tube, thereby blocking the test. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of a glucose clamp test device based on a negative feedback model of the present invention;

[0019] Figure 2 It is a schematic diagram of a test box of a glucose clamp test device based on a negative feedback model of the present invention;

[0020] Figure 3 It is a schematic diagram of a movable clamp plate and a fixed clamp plate of a glucose clamp test device based on a negative feedback model of the present invention;

[0021] Figure 4 The present invention is a glucose clamp test device based on a negative feedback model Figure 3 Enlarged view of point A in the middle;

[0022] Figure 5 It is a schematic diagram of a slider of a glucose clamp test device based on a negative feedback model of the present invention;

[0023] Figure 6 It is a schematic diagram of the positioning sleeve of a glucose clamp test device based on a negative feedback model of the present invention when it is rotated down;

[0024] Figure 7 This is a schematic diagram of the connection tube distribution of a glucose clamp test device based on a negative feedback model of the present invention.

[0025] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0026] 1. Platform; 2. Test chamber; 3. Chamber door; 4. Fixed splint; 5. Moving splint; 6. First protective pad; 7. Second protective pad; 8. Lower connecting plate; 9. Upper connecting plate; 10. Splicing plate; 11. Threaded column; 12. Positioning sleeve; 13. Socket; 14. Slide; 15. Slider; 16. Press plate; 17. Presser foot; 18. Lower guide plate; 19. Lifting plate; 20. Limiting teeth; 21. Top connecting plate; 22. Spring; 23. Lifting column; 24. Pulling plate; 25. Limiting tooth groove; 26. Connecting rod; 27. Wire passing plate; 28. Needle handle; 29. ​​Needle; 30. Connecting tube; 31. Sleeve; 32. Guide rail; 33. Guide block; 34. Guide rod; 35. Injection mechanism; 36. Detection mechanism; 37. Host module. DETAILED DESCRIPTION

[0027] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are only examples, and those skilled in the art may think of other obvious variations.

[0028] like Figure 1-Figure 7 The device for conducting a glucose clamp test based on a negative feedback model shown in the figure comprises a platform 1, a test box 2 is fixedly installed at the front edge of the upper surface of the platform 1, box doors 3 are rotatably installed on both sides of the front end of the test box 2, a fixed clamp plate 4 is connected to the inner side of the test box 2 through an XY axis moving mechanism, an upper connecting plate 9 and a lower connecting plate 8 are fixedly connected to the upper and lower ends of the fixed clamp plate 4, a movable clamp plate 5 is connected to one side of the fixed clamp plate 4 through a plug-in mechanism, a sliding opening 14 is penetrated through the upper surface of the upper connecting plate 9, two sliders 15 are slidably inserted on the inner side of the sliding opening 14 through an elastic meshing mechanism, a pressing plate 16 is fixedly connected to the lower end of the slider 15, and presser feet 17 are fixedly connected to the two sides of the lower surface of the pressing plate 16, a needle 29 is respectively arranged below each of the two presser feet 17, and a needle handle 28 is fixedly arranged on the outer surface of the needle 29.

[0029] like Figure 2 , Figure 3 As shown, the XY axis moving mechanism includes a guide rail 32 fixedly connected to the front and rear edges of the upper surface of the test box 2, and a guide block 33 is slidably mounted on the inner side of the guide rail 32. Two guide rods 34 are fixedly connected between the front and rear guide blocks 33. The outer surface of the fixed clamping plate 4 is fixedly connected to the connecting rod 26. Two sleeves 31 are fixedly embedded on the upper end of the connecting rod 26, and the sleeves 31 are slidably sleeved on the outer surface of the connecting rod 26. When the white mouse positioned between the moving clamping plate 5 and the fixed clamping plate 4 moves, the sleeve 31 will slide forward and backward along the surface of the guide rod 34, and the guide block 33 will slide laterally along the guide rail 32. Therefore, even after positioning, the movement of the white mouse will not be completely restricted, and the force of the white mouse when moving is prevented from directly acting on the moving clamping plate 5 and the fixed clamping plate 4, thereby causing positioning looseness or structural damage.

[0030] like Figure 7 As shown, the upper end of the needle 29 is fixedly connected with a connecting tube 30, and the upper surface of the connecting rod 26 is fixedly connected with a wire plate 27 extending toward the rear, and the connecting tube 30 passes through the outer surface of the wire plate 27. The wire plate 27 is made of hard rubber material to ensure that after the connecting tube 30 passes through, the friction force at the contact position with the wire plate 27 can keep the connecting tube 30 stable and will not slide relative to the wire plate 27 due to gravity.

[0031] like Figure 1 As shown, an injection mechanism 35 is connected to the end of one of the connecting tubes 30, and a detection mechanism 36 is connected to the end of the other connecting tube 30. The injection mechanism 35 and the detection mechanism 36 are respectively fixedly mounted on the upper surface of the platform 1, and a host module 37 is fixedly mounted on the upper surface of the platform 1. The host module 37 is used to control the injection mechanism 35 and the detection mechanism 36. The injection mechanism 35 is used to inject insulin, and the detection mechanism 36 is used to suck the connecting tube 30, obtain blood samples for blood sugar detection, and transmit data to the host module 37.

[0032] like Figure 3 , Figure 4 , Figure 5 As shown, the elastic engagement mechanism includes a lower guide plate 18 fixedly connected to the front and rear surfaces of the slider 15 respectively, the upper surface of the lower guide plate 18 slides against the lower surface of the upper connecting plate 9, the outer surface of the slider 15 is provided with a lifting plate 19 slidingly sleeved by an elastic mechanism, the lower surface of the lifting plate 19 is fixedly connected with a plurality of limiting teeth 20, the upper surface of the upper connecting plate 9 is provided with a limiting tooth groove 25, the limiting teeth 20 are snapped into the inner side of the limiting tooth groove 25 to achieve engagement to position the slider 15.

[0033] The elastic mechanism includes a top connecting plate 21 fixedly connected to the upper end of the slider 15, a spring 22 is fixedly connected to the lower surface of the top connecting plate 21, the lower end of the spring 22 is fixedly connected to the upper surface of the lifting plate 19, a plurality of lifting columns 23 are fixedly connected to the upper surface of the lifting plate 19, the lifting columns 23 slide through the lower surface of the top connecting plate 21, and the upper ends of the plurality of lifting columns 23 are commonly fixedly connected with a pulling plate 24, which facilitates the user to lift the lowering plate 19.

[0034] like Figure 6 As shown, the lower end of the movable clamping plate 5 is fixedly connected with a splicing plate 10, and a socket 13 is provided at the edge of the lower surface of the splicing plate 10. The lower surface of the lower connecting plate 8 is fixedly connected with a threaded column 11, which slides through the inner side of the socket 13. The lower end of the threaded column 11 is threadedly sleeved with a positioning sleeve 12, and the upper surface of the positioning sleeve 12 contacts the lower surface of the splicing plate 10. By rotating, the positioning sleeve 12 can be removed, and the movable clamping plate 5 can be separated from the fixed clamping plate 4.

[0035] like Figure 6 As shown, the inner wall of the fixed clamping plate 4 and the upper surface of the lower connecting plate 8 are fixedly connected with a first pad 6, and the inner wall of the movable clamping plate 5 is fixedly connected with a second pad 7. The first pad 6 and the second pad 7 are both made of memory foam material to make the mouse more stable when positioned.

[0036] After the user lowers the back of the mouse, inserts two needles 29 into the carotid artery of the mouse, puts them into the inner side of the fixed clamping plate 4, and closes the movable clamping plate 5. During this process, the threaded column 11 slides in relatively along the inner side of the socket 13. After sliding in, the positioning sleeve 12 is screwed on so that the upper end of the positioning sleeve 12 is tightly against the lower surface of the splicing plate 10 to complete the limit. At this time, the mouse is positioned between the movable clamping plate 5 and the fixed clamping plate 4, and then the pulling plate 24 is lifted up to make the limiting tooth 20 disengage from the inner side of the limiting tooth groove 25, and the slider 15 is moved in the horizontal direction until the two pressure feet 17 on each side are respectively pressed against the surface of the needle handle 28. After releasing the hand, under the elastic force of the spring leaf 22, the pressure foot 17 stably presses against the needle handle 28 to prevent the needle 29 from loosening. It has the function of positioning the mouse. There is no need to anesthetize or tie up the mouse in the experiment. The operation is convenient, and the inserted needle 29 can be firmly positioned to prevent the problem of separation and loosening.

[0037] When arranging the white mouse, the connecting tube 30 is pulled upward relative to the wire plate 27 so that the connecting tube 30 does not protrude too much near the bottom of the test box 2. At the same time, due to the clamping effect of the fixed clamp 4 and the movable clamp 5, the movement range of the front and rear legs of the white mouse can be effectively limited. In general, the problem of the hind legs of the white mouse touching the connecting tube 30 can be effectively prevented.

[0038] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions only describe the principles of the present invention. The present invention may be subject to various changes and improvements without departing from the spirit and scope of the present invention. These changes and improvements fall within the scope of the present invention. The scope of protection claimed by the present invention is defined by the attached claims and their equivalents.

Claims

1. A glucose clamp test device based on a negative feedback model, comprising a platform (1), characterized in that: A test box (2) is fixedly installed at the front edge of the upper surface of the platform body (1), and box doors (3) are rotatably installed on both sides of the front end of the test box (2). The inner side of the test box (2) is connected to a fixed clamping plate (4) through an XY axis moving mechanism, and the upper and lower ends of the fixed clamping plate (4) are respectively fixedly connected to an upper connecting plate (9) and a lower connecting plate (8), and one side of the fixed clamping plate (4) is connected to a movable clamping plate (5) through a plug-in mechanism. A sliding opening (14) is penetrated through the upper surface of the upper connecting plate (9), and two sliders (15) are slidably inserted into the inner side of the sliding opening (14) through an elastic meshing mechanism. The lower end of the slider (15) is fixedly connected to a pressing plate (16), and the two sides of the lower surface of the pressing plate (16) are respectively fixedly connected to presser feet (17), and needles (29) are respectively arranged below each of the two presser feet (17), and needle handles (28) are fixedly arranged on the outer surface of the needles (29).

2. The glucose clamp test device based on the negative feedback model according to claim 1, characterized in that: The XY axis moving mechanism comprises guide rails (32) fixedly connected to the front and rear edges of the upper surface of the test box (2), respectively; a guide block (33) is slidably mounted on the inner side of the guide rails (32); two guide rods (34) are fixedly connected between the front and rear guide blocks (33); the outer surface of the fixed clamping plate (4) is fixedly connected to a connecting rod (26); two sleeves (31) are fixedly embedded at the upper end of the connecting rod (26); the sleeves (31) are slidably mounted on the outer surface of the connecting rod (26).

3. A glucose clamp test device based on a negative feedback model according to claim 2, characterized in that: The upper end of the needle (29) is fixedly connected to a connecting tube (30), the upper surface of the connecting rod (26) is fixedly connected to a wire-passing plate (27) extending toward the rear, and the connecting tube (30) passes through the outer surface of the wire-passing plate (27).

4. The glucose clamp test device based on the negative feedback model according to claim 3, characterized in that: The end of one of the connecting tubes (30) is connected to an injection mechanism (35), and the end of the other connecting tube (30) is connected to a detection mechanism (36); the injection mechanism (35) and the detection mechanism (36) are respectively fixedly mounted on the upper surface of the platform (1); and a host module (37) is fixedly mounted on the upper surface of the platform (1).

5. The glucose clamp test device based on the negative feedback model according to claim 1, characterized in that: The elastic engagement mechanism comprises a lower guide plate (18) respectively fixedly connected to the front and rear surfaces of the slider (15); the upper surface of the lower guide plate (18) is slidably attached to the lower surface of the upper connecting plate (9); the outer surface of the slider (15) is provided with a lifting plate (19) via an elastic mechanism sliding sleeve; the lower surface of the lifting plate (19) is fixedly connected to a plurality of limiting teeth (20); the upper surface of the upper connecting plate (9) is provided with a limiting tooth groove (25); the limiting teeth (20) are snapped into the inner side of the limiting tooth groove (25).

6. The device for performing a glucose clamp test based on a negative feedback model according to claim 5, characterized in that: The elastic mechanism comprises a top-connecting plate (21) fixedly connected to the upper end of the slider (15); a spring sheet (22) is fixedly connected to the lower surface of the top-connecting plate (21); the lower end of the spring sheet (22) is fixedly connected to the upper surface of the lifting plate (19); a plurality of lifting columns (23) are fixedly connected to the upper surface of the lifting plate (19); the lifting columns (23) slide through the lower surface of the top-connecting plate (21); and the upper ends of the plurality of lifting columns (23) are commonly fixedly connected to a pulling plate (24).

7. The device for performing a glucose clamp test based on a negative feedback model according to claim 1, characterized in that: The lower end of the movable clamping plate (5) is fixedly connected to a splicing plate (10), and a socket (13) is formed through the edge of the lower surface of the splicing plate (10). The lower surface of the lower connecting plate (8) is fixedly connected to a threaded column (11), and the threaded column (11) slides through the inner side of the socket (13). The lower end of the threaded column (11) is threadedly sleeved with a positioning sleeve (12), and the upper surface of the positioning sleeve (12) contacts the lower surface of the splicing plate (10).

8. The device for performing a glucose clamp test based on a negative feedback model according to claim 1, characterized in that: The inner wall of the fixed clamping plate (4) and the upper surface of the lower connecting plate (8) are jointly fixedly connected with a first protective pad (6), and the inner wall of the movable clamping plate (5) is fixedly connected with a second protective pad (7).

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