Extraction and separation process of elephantopus scaber crude product

Abstract

The invention provides an extraction and separation process of an elephantopus scaber crude product, and relates to the technical field of extraction of effective components of traditional Chinese medicines. The method comprises the following steps: 1) mixing elephantopus scaber with high-concentration ethanol, and carrying out reflux extraction to obtain an extracting solution; 2) standing the extracting solution overnight, and filtering to obtain filtrate; (3) decolorizing by using macroporous resin adsorption to obtain a decolorized extracting solution; and 4) concentrating and drying the decolorized extracting solution to obtain an elephantopus scaber crude product. According to the method, the pigment can be completely removed, and the preparation process is simple and has a relatively strong industrial application prospect.

Description

Technical Field

[0001] The invention relates to an extraction and separation process of a crude product of Eleutherodactylum japonicum and application thereof, belonging to the field of medicine. Background Art

[0002] The chemical composition of the herb is rich, mainly including sesquiterpenoid lactones, triterpenoids, flavonoids, sterols and other ingredients. It is often used to treat various inflammatory diseases such as acute pharyngitis, acute gastroenteritis, acute otitis media, icteric hepatitis, nephritic edema, lung heat cough, urinary tract infection, bacillary dysentery, etc. It also has good effects in treating stomach problems, toothache, dysentery, hepatitis and other diseases. At the same time, the herb is an important component of clinically used drugs such as the compound preparation "Fuyanjing Tablets" and the in-hospital preparation "Ganmao Tea Granules". These properties make it an ideal source for seeking plant extracts or effective active ingredients.

[0003] And in the technical field, although for the extracting method of Herba Lycopodii or the chemical extraction method that is applicable are numerous, the extract that different methods obtain is all to have difference in the content of effective ingredient or effective ingredient, causes the technology that is not suitable for industrialized production at present.And, extract has passed through leaching process, also can cause water content to be higher, thus need to carry out decolouring, drying and other operations just can ensure product quality.Therefore, for the extraction of Herba Lycopodii, be in the urgent need of a kind of extraction process of unified standard, can extract the extract with pharmacological activity, can not only remove pigment to ensure product quality, be more suitable for later stage industrialized production. Summary of the invention

[0004] In view of this, the object of the present invention is to provide an extraction and separation process for a crude product of Eleutherodactyl oxyphylla to improve the safety of the target components and make it more suitable for later industrial production.

[0005] In order to achieve the above-mentioned purpose of the invention, the present invention provides an extraction and separation process of a crude product of Eleutherodactylus aviculare, wherein the crude product of Eleutherodactylus aviculare is an ethanol extract of Eleutherodactylus aviculare, and the process includes a decolorization process.

[0006] At present, relevant purification techniques have been developed for Eleutherodactylus aviculare, but they are all in the laboratory stage and cannot be applied to industrial production. In the course of research, it was found that if the decolorization process is not adopted, the efficacy of the crude extract of Eleutherodactylus aviculare would be greatly affected.

[0007] Preferably, in the above extraction and separation process, the Herba Lycopodii includes the whole Herba Lycopodii, the Herba Lycopodii rhizomes, the Herba Lycopodii leaves or the Herba Lycopodii flowers.

[0008] Preferably, in the above-mentioned extraction and separation process, the extraction process includes the following steps: 1) ethanol extraction; 2) standing and filtering to obtain a filtrate; 3) decolorizing and adsorbing the filtrate to obtain a decolorized extract; 4) concentrating and drying the decolorized extract obtained in step 3) to obtain the crude product of the herb.

[0009] Preferably, in the above extraction and separation process, the ethanol is a 50%-95% ethanol aqueous solution.

[0010] Preferably, in the above extraction and separation process, the decolorization adsorption process is macroporous resin adsorption.

[0011] Preferably, in the above extraction and separation process, the filler of the macroporous resin in the decolorization and adsorption process is a macroporous resin of the D series, HPD series, HP series, XAD series or AB series, preferably the AB series.

[0012] Preferably, the above-mentioned extraction and separation process includes the following steps: 1) mixing the herb with high-concentration ethanol, performing reflux extraction, and obtaining an extract; the high-concentration ethanol is 50%-95% ethanol; 2) allowing the extract obtained in step 1) to stand overnight, and filtering to obtain a filtrate; 3) using a macroporous resin to adsorb the filtrate obtained in step 2) to decolorize, and obtain a decolorized extract; 4) concentrating and drying the decolorized extract obtained in step 3) to obtain a crude herb product.

[0013] The present invention further provides a crude product of Eleutherodactyla scabra prepared by the extraction and separation process.

[0014] The present invention further provides an application of the above-mentioned Eleutherodactyla product in the preparation of atopic dermatitis medicine.

[0015] Preferably, in step 1), the volume ratio of the mixture of the Eleutherodactyl and high-concentration ethanol is 1:5 to 1:20.

[0016] Preferably, the extract obtained in step 2) is allowed to stand for 12 to 48 hours.

[0017] Preferably, the filler model of the macroporous resin in step 3) is AB series.

[0018] Preferably, the concentration in step 4) is to concentrate to a relative density of 1.00 to 1.20.

[0019] The invention provides an extraction and separation process of a crude product of Eleutherodactylus scabra. During the extraction process, non-target components such as plant protein, gum (pectin), starch, plant fiber and wood, pesticide residues and heavy metals, pigments (chlorophyll, lutein, melanin, etc.) and other trace elements are removed in sequence. The prepared crude product of Eleutherodactylus scabra containing medicinal ingredients for treating atopic dermatitis can not only remove pigments to ensure product quality, but also is more suitable for later industrial production.

[0020] The invention provides an extraction and separation process of a crude product of Eleutherodactylus spp., comprising the following steps: 1) mixing the Eleutherodactylus spp. with high-concentration ethanol, performing reflux extraction, and obtaining an extract; the high-concentration ethanol is 50% to 95% ethanol; 2) allowing the extract obtained in step 1) to stand overnight, and filtering to obtain a filtrate; 3) decolorizing the filtrate obtained in step 2) by adsorbing a macroporous resin, and obtaining a decolorized extract; 4) concentrating and drying the decolorized extract obtained in step 3) to obtain a crude product of Eleutherodactylus spp.

[0021] In the extraction method of the crude product of the present invention, the scutellaria baicalensis is mixed with high-concentration ethanol, and reflux extraction is performed to obtain an extract. The scutellaria baicalensis of the present invention preferably includes the whole plant of the scutellaria baicalensis, the rhizome of the scutellaria baicalensis, the leaves of the scutellaria baicalensis or the flowers of the scutellaria baicalensis. The source of the scutellaria baicalensis is not particularly limited in the present invention, and the whole plant of the scutellaria baicalensis is preferably used. The volume ratio of the scutellaria baicalensis and the high-concentration ethanol mixed in the present invention is 1:5 to 1:20, more preferably 1:10 to 1:15, and most preferably 1:15. The number of reflux extractions of the present invention is 1 to 5 times, more preferably 1 to 3 times, and most preferably 2 times. The time of the reflux extraction of the present invention is preferably 1 to 4h, more preferably 1 to 2h, and most preferably 1h. The high-concentration ethanol used in the reflux extraction of the present invention is 50% to 95% ethanol, more preferably 50% to 95% ethanol, and most preferably 85% ethanol.

[0022] The extract obtained by reflux extraction is allowed to stand overnight, and then filtered to obtain a filtrate. In the present invention, the extract is allowed to stand for 12 to 48 hours, more preferably 12 to 24 hours, and most preferably 24 hours. The mesh size of the filter screen for filtration in the present invention is preferably 80 to 200 meshes, more preferably 100 to 150 meshes, and most preferably 120 meshes.

[0023] The filtrate is adsorbed by a macroporous resin for decolorization to obtain a decolorized extract. The resin of the present invention preferably includes a polar resin, and more preferably includes an AB-8 macroporous resin. The present invention does not specifically limit the source of the resin, and conventional commercial products in the field can be used. The resin of the present invention can adsorb the pigment in the non-flocculated extract, and allow pesticide residues, heavy metals, sugar compounds, etc. to flow out naturally through the aqueous phase to achieve a removal effect. In order to increase the product yield, a small amount of eluent is required to elute the resin that adsorbs the extract. The eluent of the present invention includes an ethanol aqueous solution, and the volume fraction of the ethanol aqueous solution is preferably 50-95%, more preferably 75-95%, and most preferably 85%. The decolorization of the present invention is preferably to decolorize the generated stable solution through a resin to remove pigments such as chlorophyll, lutein and melanin.

[0024] The obtained decolorized extract is concentrated and dried to obtain a crude product of the herb. The removal of the present invention is preferably to recycle high-concentration ethanol water, and the recycling of ethanol water and the concentration are preferably carried out in a vacuum concentration tank, and the vacuum degree during the recycling and concentration is preferably 0.02-0.09M pa, more preferably 0.0 8-0.09M pa. The recycling and concentration of the present invention are preferably concentrated to a relative density of 1.10-1.30 (relative density is reasonably controlled according to the selected drying method). The present invention is not particularly limited to the drying method, and the conventional method in the art can be used.

[0025] Another object of the present invention is to provide an anti-inflammatory composition, which is composed of an effective dose of the Herba Lysimachiae extract of the present invention and a pharmaceutically acceptable excipient. The pharmaceutically acceptable excipient refers to an excipient required for making any pharmaceutical dosage form suitable for human or animal use. For example, when making an oral solid preparation, the pharmaceutically acceptable excipient refers to a diluent, a binder, a wetting agent, a disintegrant, a lubricant, and a glidant; when making an injection, the pharmaceutically acceptable excipient refers to a pH regulator, a cosolvent, an antioxidant, an isotonic agent, etc. The drug refers to a tablet, a pill, a capsule, a suspension, an ointment or an emulsion, etc.

[0026] The results of in vivo pharmacodynamic tests show that the crude product of Eleutherodactyla scabra of the present invention has anti-atopic dermatitis activity, and different concentrations of the crude product of Eleutherodactyla scabra can show excellent improvement effects on MC903-induced atopic dermatitis mice.

[0027] Therefore, the present invention further provides the use of the above-mentioned crude product of Eleutherodactyla scabra in the preparation of anti-atopic dermatitis drugs.

[0028] Once again, it is reiterated that the following experiments are only exemplary experiments among the many experiments conducted during the research and development of the present invention, and do not cover and exhaust all the experiments conducted by the inventor for the present invention. The purpose is only to use those data to illustrate the anti-atopic dermatitis activity of the crude product of Eleutherococcus scoparia of the present invention. DETAILED DESCRIPTION

[0029] Example 1

[0030] 20kg of the whole grass of the Herba Epimedii is put into the extraction tank, 300L of 85% ethanol is added, and it is heated to boiling. Keep boiling extraction for 1h, extract twice, combine the extract while hot, put it to room temperature and let it stand for 24h. After standing, filter and collect the filtrate, adsorb the filtrate on the AB-8 macroporous resin that meets the polarity range of the Herba Epimedii active ingredients, and desorb the target component containing pigment on the macroporous resin with 85% edible ethanol to obtain a decolorized eluent. The decolorized eluent is placed in a vacuum concentration tank to recycle the edible ethanol, concentrated to a relative density of 1.15, collect the concentrate, dry, remove the aqueous phase, and obtain the crude product of the Herba Epimedii. 1.6kg of the crude product extract of the Herba Epimedii prepared by this method has an extraction rate of 8%.

[0031] Example 2

[0032] 20kg of raw materials of stems and leaves of the Herba Lycopodii were put into an extraction tank, 240L of 95% ethanol was added, and the mixture was heated to boiling. The boiling extraction was maintained for 1h, and the extraction was performed twice. The extracts were combined while hot, and the mixture was placed at room temperature for 24h. After standing, the mixture was filtered and the filtrate was collected. The filtrate was adsorbed on an AB-8 macroporous resin within the polarity range of the Herba Lycopodii active ingredients, and the target component containing pigment on the macroporous resin was desorbed with 95% edible ethanol to obtain a decolorized eluent. The decolorized eluent was placed in a vacuum concentration tank to recover the edible ethanol, and the edible ethanol was concentrated to a relative density of 1.10, and the concentrated solution was collected, dried, and the aqueous phase was removed to obtain the crude product of the Herba Lycopodii. 1.4kg of the crude product extract of the Herba Lycopodii prepared by the present method was extracted with an extraction rate of 7%.

[0033] Example 3

[0034] 20kg of the whole grass of the Herba Epimedii is put into an extraction tank, 300L of 75% ethanol is added, and it is heated to boiling. The boiling extraction is maintained for 1h, and the extraction is performed twice. The extract is combined while hot, and the extract is placed at room temperature for 24h. After standing, the extract is filtered and the filtrate is collected. The filtrate is adsorbed on an AB-8 macroporous resin within the polarity range of the Herba Epimedii active ingredients, and the target component containing the pigment on the macroporous resin is desorbed with 75% edible ethanol to obtain a decolorized eluent. The decolorized eluent is placed in a vacuum concentration tank to recycle the edible ethanol, and is concentrated to a relative density of 1.20, and the concentrated solution is collected, dried, and the aqueous phase is removed to obtain the crude product of the Herba Epimedii. 1.8kg of the crude product extract of the Herba Epimedii prepared by this method has an extraction rate of 9%.

[0035] Example 4

[0036] 20kg of the raw material of the stem and leaf of the Herba Lycopodiellae was put into an extraction tank, 300L of 85% ethanol was added, and the mixture was heated to boiling. The boiling extraction was maintained for 2h, and the extraction was performed twice. The extract was combined while hot, and the mixture was placed at room temperature for 24h. After standing, the mixture was filtered and the filtrate was collected. The filtrate was adsorbed on a HP-20 macroporous resin within the polarity range of the Herba Lycopodiellae active ingredients, and the target component containing the pigment on the macroporous resin was desorbed with 85% edible ethanol to obtain a decolorized eluent. The decolorized eluent was placed in a vacuum concentration tank and the edible ethanol was recovered, and the mixture was concentrated to a relative density of 1.25, and the concentrated solution was collected, dried, and the aqueous phase was removed to obtain the crude product of the Herba Lycopodiellae. 1.7kg of the crude product extract of the Herba Lycopodiellae prepared by this method had an extraction rate of 8.5%.

[0037] Example 5

[0038] 20kg of the raw material of the stem and leaf of the Herba Lycopodiellae was put into an extraction tank, 300L of 95% ethanol was added, and the mixture was heated to boiling. The boiling extraction was maintained for 2h, and the extraction was performed twice. The extract was combined while hot, and the mixture was placed at room temperature for 24h. After standing, the mixture was filtered and the filtrate was collected. The filtrate was adsorbed on a HP-20 macroporous resin within the polarity range of the Herba Lycopodiellae active ingredients, and the target component containing the pigment on the macroporous resin was desorbed with 95% edible ethanol to obtain a decolorized eluent. The decolorized eluent was placed in a vacuum concentration tank and the edible ethanol was recovered, and the mixture was concentrated to a relative density of 1.25, and the concentrated solution was collected, dried, and the aqueous phase was removed to obtain a crude Herba Lycopodiellae product. 1.5kg of the crude Herba Lycopodiellae product extract prepared by this method had an extraction rate of 7.5%.

[0039] Example 6

[0040] 20kg of the raw material of the stem and leaf of the Herba Lycopodiellae was put into an extraction tank, 300L of 75% ethanol was added, and the mixture was heated to boiling. The boiling extraction was maintained for 2h, and the extraction was performed twice. The extract was combined while hot, and the mixture was placed at room temperature for 24h. After standing, the mixture was filtered and the filtrate was collected. The filtrate was adsorbed on a HP-20 macroporous resin within the polarity range of the Herba Lycopodiellae active ingredients, and the target component containing the pigment on the macroporous resin was desorbed with 75% edible ethanol to obtain a decolorized eluent. The decolorized eluent was placed in a vacuum concentration tank and the edible ethanol was recovered, and the mixture was concentrated to a relative density of 1.25, and the concentrated solution was collected, dried, and the aqueous phase was removed to obtain a crude Herba Lycopodiellae product. 2kg of the crude Herba Lycopodiellae product extract prepared by this method had an extraction rate of 10%.

[0041] Example 7

[0042] 20kg of the raw material of the stem and leaf of the Herba Lycopodiellae was put into an extraction tank, 400L of 85% ethanol was added, and the mixture was heated to boiling. The boiling extraction was maintained for 2h, and the extraction was performed 3 times. The extract was combined while hot, and the mixture was placed at room temperature for 48h. After standing, the mixture was filtered and the filtrate was collected. The filtrate was adsorbed on a D101 macroporous resin within the polarity range of the Herba Lycopodiellae active ingredients, and the target component containing the pigment on the macroporous resin was desorbed with 85% edible ethanol to obtain a decolorized eluent. The decolorized eluent was placed in a vacuum concentration tank and the edible ethanol was recovered, and the mixture was concentrated to a relative density of 1.20, and the concentrated solution was collected, dried, and the aqueous phase was removed to obtain the crude product of the Herba Lycopodiellae. 1.85kg of the crude product extract of the Herba Lycopodiellae prepared by the present method had an extraction rate of 9.25%.

[0043] Example 8

[0044] 20kg of the raw material of the stem and leaf of the Herba Lycopodiellae was put into an extraction tank, 400L of 95% ethanol was added and heated to boiling. The boiling extraction was maintained for 2h, and the extraction was performed 3 times. The extract was combined while hot and placed at room temperature for 48h. After standing, the extract was filtered and the filtrate was collected. The filtrate was adsorbed on a D101 macroporous resin within the polarity range of the Herba Lycopodiellae active ingredients, and the target component containing the pigment on the macroporous resin was desorbed with 85% edible ethanol to obtain a decolorized eluent. The decolorized eluent was placed in a vacuum concentration tank and the edible ethanol was recovered, concentrated to a relative density of 1.30, and the concentrated solution was collected, dried, and the aqueous phase was removed to obtain the crude product of the Herba Lycopodiellae. 1.75kg of the crude product extract of the Herba Lycopodiellae prepared by this method was 8.75% in extraction yield.

[0045] Example 9

[0046] 20kg of raw materials of stem and leaf of Herba Lycopodiellae were put into the extraction tank, 400L volume of 75% ethanol was added, and the mixture was heated to boiling. The boiling extraction was maintained for 2h, and the extraction was performed 3 times. The extract was combined while hot, and the mixture was placed at room temperature for 48h. After standing, the mixture was filtered and the filtrate was collected. The filtrate was adsorbed on a D101 macroporous resin within the polarity range of the effective components of Herba Lycopodiellae, and the target component containing pigment on the macroporous resin was desorbed with 85% edible ethanol to obtain a decolorized eluent. The decolorized eluent was placed in a vacuum concentration tank and the edible ethanol was recovered completely, and the mixture was concentrated to a relative density of 1.30, and the concentrated solution was collected, dried, and the aqueous phase was removed to obtain the crude product of Herba Lycopodiellae. 2.2kg of the crude product extract of Herba Lycopodiellae prepared by the present method was 11% in extraction yield.

[0047] Example 10

[0048] 20kg of the whole grass of the Herba Epimedii is put into an extraction tank, 300L of 50% ethanol is added, and it is heated to boiling. Keep boiling extraction for 1h, extract twice, combine the extract while hot, put it to room temperature and let it stand for 24h. After standing, filter and collect the filtrate, adsorb the filtrate on the AB-8 macroporous resin that meets the polarity range of the Herba Epimedii active ingredients, and desorb the target component containing pigment on the macroporous resin with 75% edible ethanol to obtain a decolorized eluent. The decolorized eluent is placed in a vacuum concentration tank to recycle the edible ethanol, concentrated to a relative density of 1.15, collect the concentrate, dry, remove the aqueous phase, and obtain the Herba Epimedii crude product. 2kg of the Herba Epimedii crude product extract prepared by this method has an extraction rate of 10%.

[0049] Embodiment 11

[0050] 20kg of the whole grass of the Herba Epimedii is put into an extraction tank, 400L of 50% ethanol is added, and it is heated to boiling. The boiling extraction is maintained for 2h, and the extraction is performed twice. The extract is combined while hot, and the extract is placed at room temperature for 48h. After standing, the extract is filtered and the filtrate is collected. The filtrate is adsorbed on a HP-20 macroporous resin within the polarity range of the Herba Epimedii active ingredients, and the target component containing the pigment on the macroporous resin is desorbed with 75% edible ethanol to obtain a decolorized eluent. The decolorized eluent is placed in a vacuum concentration tank to recycle the edible ethanol, and is concentrated to a relative density of 1.20, and the concentrated solution is collected, dried, and the aqueous phase is removed to obtain a crude Herba Epimedii product. 2.3kg of the crude Herba Epimedii product extract prepared by this method has an extraction rate of 11.5%.

[0051] Example 12

[0052] 20kg of the whole grass of the Herba Epimedii is put into an extraction tank, 400L of 50% ethanol is added, and it is heated to boiling. The boiling extraction is maintained for 2h, and the extraction is performed 3 times. The extract is combined while hot, and the extract is placed at room temperature for 24h. After standing, the extract is filtered and the filtrate is collected. The filtrate is adsorbed on a HP-20 macroporous resin within the polarity range of the Herba Epimedii active ingredients, and the target component containing the pigment on the macroporous resin is desorbed with 85% edible ethanol to obtain a decolorized eluent. The decolorized eluent is placed in a vacuum concentration tank to recycle the edible ethanol, and is concentrated to a relative density of 1.30. The concentrated solution is collected, dried, and the aqueous phase is removed to obtain the crude product of the Herba Epimedii. 2.4kg of the crude product extract of the Herba Epimedii prepared by this method has an extraction rate of 12%.

[0053] Example 13 Preparation of undecolorized sample

[0054] 20 kg of the whole grass of the scutellaria baicalensis raw material was put into an extraction tank, 300 L of 85% ethanol was added, and the mixture was heated to boiling. The boiling extraction was maintained for 1 h, and the extraction was repeated twice. The hot combined extract was placed in a vacuum concentration tank to recover the edible ethanol, and the extract was concentrated to a relative density of 1.15. The concentrate was collected, dried, and the aqueous phase was removed to obtain the crude product of the scutellaria baicalensis. 1.7 kg of the crude product extract of the scutellaria baicalensis prepared by the method had an extraction rate of 8.5%.

[0055] Example 14 Undecolorized sample using activated carbon decolorization

[0056] The method is the same as that of Example 13. The obtained Eleutherodactylus scabra concentrated solution is mixed with activated carbon at a mass ratio of 10:1. After adsorption, the mixture is dried to obtain 1.3 kg of Eleutherodactylus scabra crude product extract with an extraction rate of 6.5%.

[0057] Embodiment 15

[0058] Experimental study on the anti-atopic dermatitis activity of crude product of Herba Lysimachiae

[0059] 1. Materials and Animal Grouping

[0060] 1.1 Experimental animals

[0061] BALB / c mice, SPF grade, weighing 18-22 g, were purchased from Beijing Weitonglihua Experimental Animal Technology Co., Ltd., license number: SCXK (Beijing) 2016-0009. Mice were group-housed with 6 mice per cage, free access to food and water, room temperature of 23-25℃, humidity of 50±10%, and light from 7:00 to 19:00. The experiment began after the animals were adapted to the new environment for 3 days.

[0062] 1.2 Experimental instruments and reagents

[0063] AE100 electronic balance (Mettler-Toledo Instruments Shanghai Co., Ltd.); T520 electronic balance (Xiangyi, Changsha, Hunan); C-YLS-Q4 ear puncher (Beijing Zhecheng Technology Co., Ltd.); anhydrous ethanol (Tianjin Beilian Fine Chemicals Development Co., Ltd.)

[0064] 1.3 Experimental samples

[0065] See Examples 1 to 14, wherein Example 13 is an undecolorized sample, and Example 14 is an activated carbon decolorized sample.

[0066] 1.4 Sample concentration setting

[0067] The concentration of the crude extract of Eleutherodactyla scabra was 5% (5 g / 100 ml).

[0068] 1.5 Data processing and statistical analysis

[0069] The experimental data were expressed as (mean ± standard deviation). SPSS17.0 data processing software was used for statistical analysis, and t-test and q value were used for inter-group comparison. When p < 0.05, there was a significant difference between the groups.

[0070] 2 Experimental methods

[0071] On the morning of the formal experiment, except for the mice in the normal control group, 0.1mM MC903 (dissolved in anhydrous ethanol) was applied to the left and right ears of each mouse, 10μl / ear; 10 minutes later, 10μL of anhydrous ethanol was applied to the left and right ears of the mice in the normal control group and the model group; 10μL of anhydrous ethanol was applied to the left ear of the mice in the drug administration group, and 10μL of 5% crude product of local gall grass (dissolved in anhydrous ethanol) was applied to the right ear. This operation was performed once a day, and the growth status of the mice was monitored during the period. After 14 days, the severity of the skin lesions of the mice was observed, and the mice were euthanized. The ear pieces with a diameter of 8mm were taken from the same position of the left and right ears and weighed.

[0072] Effects of 3-Dancao crude product on BALB / c mice with atopic dermatitis

[0073] After giving modeling agent MC903, it can be seen that the skin lesions of mouse ear are serious, and typical atopic dermatitis symptoms such as obvious edema, erythema, epidermal exfoliation, incrustation, lichenification and dryness occur. And after giving 12 thick products of Herba Lysimachiae, dermatitis conditions are all improved to varying degrees (Table 1), wherein Example 1 has the best effect, and the ear conditions of this group of mice are almost not much different from the normal control group. The most surprising thing is that the drug effect of the sample after decolorization (the thick product of embodiment 1-12) is obviously better than that of the undecolorized sample (embodiment 13), and the drug effect of the sample (embodiment 14) decolorized with activated carbon is very poor instead.

[0074] Table 1 Effect of crude product of Eleutherococcus serrata on ear weight of mice with atopic dermatitis induced by MC903 (n=6)

[0075]

[0076] The present invention is not limited to the above-mentioned embodiments, and any other products identical or similar to the present invention obtained by anyone under the inspiration of the present invention are not excluded from the protection scope of the present invention.

Claims

1. A process for extracting and separating a crude product of ophiopogon japonicus, characterized in that: The crude product of Eleutherodactylus scabra is an ethanol extract of Eleutherodactylus scabra, which includes a decolorization process.

2. The extraction and separation process according to claim 1, characterized in that: The described scutellaria baicalensis includes the whole plant of scutellaria baicalensis, the rhizome of scutellaria baicalensis, the leaf of scutellaria baicalensis or the flower of scutellaria baicalensis.

3. The extraction and separation process according to claim 1, characterized in that: The extraction process comprises the following steps: 1) ethanol extraction; 2) standing and filtering to obtain a filtrate; 3) decolorizing and adsorbing the filtrate to obtain a decolorized extract; 4) concentrating and drying the decolorized extract obtained in step 3) to obtain the crude product of the herb.

4. The extraction and separation process according to claim 3, characterized in that: The ethanol is a 50%-95% ethanol aqueous solution.

5. The extraction and separation process according to claim 3, characterized in that: The decolorization adsorption process is macroporous resin adsorption.

6. The extraction and separation process according to claim 5, characterized in that: The filler of the macroporous resin in the decolorization adsorption process is a macroporous resin of the D series, HPD series, HP series, XAD series or AB series, preferably the AB series.

7. The extraction and separation process according to claim 1, characterized in that: The following steps are involved: 1) mixing the herb with high-concentration ethanol, and performing reflux extraction to obtain an extract; the high-concentration ethanol is 50%-95% ethanol; 2) leaving the extract obtained in step 1) to stand overnight and filtering to obtain a filtrate; 3) decolorizing the filtrate obtained in step 2) by adsorbing it with a macroporous resin to obtain a decolorized extract; 4) The decolorized extract obtained in step 3) is concentrated and dried to obtain a crude product of Eleutherodactyla scabra.

8. A crude product of Eleutherodactyla scabra prepared by the extraction and separation process according to any one of claims 1 to 7.

9. Use of the Eleutherodactyla product according to claim 8 in the preparation of atopic dermatitis drugs.

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