Antibacterial cream containing snail protease and herbal extract and preparation method thereof

Abstract

The invention discloses a bacteriostatic cream containing snail protease and a herbal extract and a preparation method thereof, and belongs to the technical field of external medicament processing, and the bacteriostatic cream enhances the bacteriostatic performance through combination of the herbal extract and the snail protease in an accurate ratio and assistance of a specific modified additive. The preparation method comprises the following steps: extracting the herbal extract, preparing a snail protease solution, mixing the herbal extract and the snail protease solution, adding the oil phase and the emulsifier for homogenization, and finally adding chlorhexidine acetate to obtain the bacteriostatic cream. Compared with the prior art, the cream provided by the invention has good storage stability and skin compatibility, shows an excellent antibacterial effect through tests, is non-irritant to skin, and is suitable for wide skin antibacterial requirements.

Description

Technical Field

[0001] The invention relates to the technical field of external medicament processing, in particular to an antibacterial cream containing snail protease and herbal extract and a preparation method thereof. Background Art

[0002] With the acceleration of modern life and the increasing prominence of environmental problems, skin problems have become one of the health issues that people generally pay attention to. Among many skin problems, bacterial infection is particularly common, which not only affects the appearance, but may also cause more serious health problems. Therefore, the development of an effective antibacterial product is of great significance for the prevention and treatment of bacterial skin infections.

[0003] In the field of topical drug processing technology, a variety of antibacterial cream products have been widely used. These products usually contain chemically synthesized antibacterial agents. Although they have certain antibacterial effects, long-term use may lead to drug resistance problems and may cause irritation to the skin. In addition, although traditional Chinese medicine compounds have good antibacterial effects and low side effects, their preparation methods are often more complicated, and their storage stability and ease of use need to be improved.

[0004] Chinese invention patent CN118490777A discloses a Chinese medicine compound moisturizing, repairing and antibacterial cream and its preparation method. The preparation method of the cream comprises the following steps: preparation of a Chinese medicine compound extract; preparation of a pre-cured antibacterial gel; and a thermosensitive gel modified cream. The invention prepares a thermosensitive gel, and then mixes it with the pre-cured antibacterial gel, an oil phase and an aqueous phase to prepare a cream. The Chinese medicine compound extract can be continuously and slowly released, so that the effective ingredients maintain a suitable concentration in the treatment area, and provide continuous and stable treatment while reducing the irritation to the wound. A small amount of cream can be directly added to hot water for stirring. At this time, the thermosensitive gel will shrink rapidly at high temperature, so that the Chinese medicine compound extract is squeezed out and dissolved in hot water, so that the antibacterial components are quickly and evenly distributed in the aqueous solution, so that it can maintain high antibacterial ability after spraying or applying, which is convenient and practical. However, the storage and antibacterial properties of the Chinese medicine compound moisturizing, repairing and antibacterial cream prepared by the invention need to be improved. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention aims to provide an antibacterial cream containing snail protease and herbal extracts and a preparation method thereof, so as to improve the storage stability and skin compatibility of the product while maintaining excellent antibacterial effects.

[0006] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions:

[0007] A method for preparing an antibacterial cream containing snail protease and herbal extract is as follows, in parts by weight:

[0008] 0.5-1 part of honeysuckle, 1-2 parts of cnidium monnieri, 0.4-0.6 part of sophora flavescens, 1-2 parts of radix stemonae, 1.5-2.5 parts of cortex difficile, and 0.8-1.2 parts of fructus kochiae are decocted and extracted with 50-70 parts of water to obtain an extract; the extract is filtered and concentrated to obtain a herbal extract; 0.3-0.5 parts of snail protease are added to 1-3 parts of water to obtain a snail protease solution; 0.8-1.2 parts of borneol and 0.4- 0.6 parts of menthol are added to 8-12 parts of ethanol to obtain a borneol-menthol solution; the herbal extract, the snail protease solution and the borneol-menthol solution are mixed evenly; 10-14 parts of an oil phase, 1-3 parts of an emulsifier and 0.8-1.2 parts of a modifying additive are added, and homogenization is performed at 5000-10000 rpm to obtain a cream base; 0.1-0.3 parts of chlorhexidine acetate are added to the cream base, and the mixture is mixed evenly to obtain an antibacterial cream.

[0009] The extract is filtered through a 200-400 mesh filter cloth.

[0010] The concentration is to concentrate at 70-90° C. to a density of 1.2-1.3 g / mL.

[0011] The oil phase is at least one of beeswax, glyceryl monostearate, liquid paraffin and lanolin.

[0012] The oil phase is composed of beeswax, glyceryl monostearate, liquid paraffin and lanolin in a mass ratio of 1-3:4-6:7-9:4-6.

[0013] The emulsifier is at least one of Span 60 and glyceryl macrogol-75 stearate.

[0014] The emulsifier is composed of Span 60 and glycerol macrogol-75 stearate in a mass ratio of 1 to 3:1.

[0015] The preparation method of the modified additive is as follows, in parts by weight:

[0016] S1. Mix 0.8-1.2 parts of hydroxypropyl deacetylated chitosan with 15-25 parts of water, stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 60-70° C., and gradually add 2-4 parts of locust bean gum at a stirring speed of 300-500 rpm, and continue stirring for 1-3 hours to form a polysaccharide complex;

[0017] S2. Mix 1 to 2 parts of ginseng extract with the polysaccharide complex in step S1, add 1 to 2 parts of acacia aldehyde, adjust the pH value to 5 to 6.5 with a 0.5 to 2 mol / L acetic acid aqueous solution, treat at a temperature of 50 to 70° C. for 2 to 4 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 400 to 800 bar for 3 to 8 minutes to obtain a modified additive.

[0018] The functions of each substance in the present invention are as follows:

[0019] Herbal extracts of honeysuckle, cnidium monnieri, sophora flavescens, radix stemonae, radix difficile, and kochia scoparia provide the cream’s natural antibacterial and anti-inflammatory properties, as well as possible skin repair and soothing effects. They are traditional Chinese medicines used to treat a variety of skin problems.

[0020] Snail protease may help promote skin cell renewal and repair due to its enzymatic activity and may also have some antimicrobial effects.

[0021] Ingredients such as borneol and menthol are often used to provide a cooling sensation and relieve skin discomfort, such as itching and burning.

[0022] Ethanol as a solvent. Ethanol is used to dissolve borneol and menthol, and also helps preserve and stabilize the active ingredients in the cream.

[0023] The oil phase provides essential lubrication and protection to the cream, helps lock in moisture, keeps the skin soft, and acts as a carrier for active ingredients.

[0024] Emulsifiers are used to mix the oil phase and the water phase to form a uniform cream, ensure the stability of the cream and extend the shelf life.

[0025] The modified additives enhanced the stability and homogeneity of the cream, provided additional skin protection and repair, and acaciaaldehyde may also enhance the antibacterial properties of the cream.

[0026] Chlorhexidine Acetate As a broad-spectrum antimicrobial agent, chlorhexidine acetate provides the primary antimicrobial activity of the cream, helping to prevent and treat bacterial skin infections.

[0027] These ingredients work together to ensure that the antibacterial cream of the present invention not only has excellent antibacterial effect, but also has good skin compatibility and storage stability.

[0028] Compared with the prior art, it has the following beneficial effects:

[0029] 1) The present invention enhances the antibacterial properties of the cream by combining snail protease and herbal extracts, as well as introducing specific modified additives. This synergistic effect enables the cream to maintain a high antibacterial activity after storage, effectively combating common bacteria such as Escherichia coli and Staphylococcus aureus.

[0030] 2) Through precise formula design and preparation process, the cream of the present invention exhibits better stability under high-temperature storage conditions, reduces the hardening and demulsification of the cream, thereby extending the shelf life and service life of the product and improving the practicality and reliability of the cream.

[0031] 3) The cream of the present invention has been tested for skin irritation, and the results show that it is non-irritating to the skin and suitable for external use. This is due to the mild ingredient selection and sophisticated preparation process, which ensures the safety and comfort of the cream, is suitable for all skin types, and reduces the risk of adverse skin reactions. DETAILED DESCRIPTION

[0032] Main sources of substances:

[0033] Honeysuckle is produced in Pingyi County, Shandong Province.

[0034] The origin of Cnidium monnieri is Haiyan County, Jiaxing, Zhejiang.

[0035] Sophora flavescens is produced in Shangluo City, Shaanxi Province.

[0036] Stemona bulbus is produced in Ningbo, Zhejiang.

[0037] The origin of Dictamni is Baoqing County, Heilongjiang Province.

[0038] The origin of Kochia scoparia is Wuchang City, Harbin, Heilongjiang Province.

[0039] Snail protease, product model: SDY-WN-98, Shanxi Shengdayue Biotechnology Co., Ltd.

[0040] Borneol is produced in Meizhou City, Guangdong Province.

[0041] Menthol is produced in Nantong, Jiangsu.

[0042] Beeswax, Model: 002, Dongguan County Jinsen Wax Products Co., Ltd.

[0043] Liquid paraffin, product grade: cosmetic grade, model: 10#, Maoming Hongtai Petrochemical Co., Ltd.

[0044] Glyceryl macrogol-75 stearate, French GELOT 64.

[0045] Hydroxypropyl chitosan, AR substitution degree ≥80.0%, product number: PA11877, Guangdong Wengjiang Chemical Reagent Co., Ltd.

[0046] Carboxymethyl chitosan, single product number: 2023052155, Shanxi Tongling Biotechnology Co., Ltd.

[0047] Pullulan, item number: 102, product grade: food grade, Jiangsu Fushengde Bioengineering Co., Ltd.

[0048] Soluble starch, product number: 207, Shandong Pingju Biotechnology Co., Ltd.

[0049] Ginseng extract, single product number: KLTD200581, Xi'an Keli Tengda Biotechnology Co., Ltd.

[0050] Locust bean gum, item number: 257L, product grade: food grade, Suzhou Baiyixin Biotechnology Co., Ltd.

[0051] Xanthan gum, product number: 6250, Hefei Jushengyuan Biotechnology Co., Ltd.

[0052] Carrageenan, product number: 0844, Jiangsu Hanmao Biotechnology Co., Ltd.

[0053] Konjac gum, item number: 003, Anhui Xinqingda Biotechnology Co., Ltd.

[0054] The remaining raw materials in the examples and comparative examples of the present invention are all commercially available products.

[0055] The design idea of ​​the present invention is to provide an antibacterial cream containing snail protease and herbal extract and a preparation method thereof, aiming to improve the deficiencies of the prior art, and enhance the storage performance and antibacterial effect of the cream by combining the accurately proportioned herbal extract with snail protease and introducing specific modified additives. The method ensures the stability and biological activity of the effective ingredients of the cream by optimizing key steps such as extraction, concentration, mixing, and homogenization, and at the same time, by selecting a specific oil phase and emulsifier, and using a high-pressure homogenization method to treat the modified additives, the uniformity and skin compatibility of the cream are improved, thereby achieving better antibacterial and care effects.

[0056] Example 1

[0057] A method for preparing an antibacterial cream containing snail protease and herbal extract is as follows:

[0058] 0.8kg of honeysuckle, 1.5kg of cnidium monnieri, 0.5kg of sophora flavescens, 1.5kg of radix stemonae, 2kg of cortex difficile and 1kg of fructus kochiae are decocted and extracted with 60kg of water to obtain an extract; the extract is filtered through a 300-mesh filter cloth and concentrated at 80°C to a density of 1.28g / mL to obtain a herbal extract; 0.4kg of snail protease is added to 2kg of water to obtain a snail protease solution; 1kg of borneol and 0.5kg of menthol are added to 10kg of ethanol to obtain a borneol-menthol solution; the herbal extract, the snail protease solution and the borneol-menthol solution are mixed evenly; 12kg of an oil phase, 2kg of an emulsifier and 1kg of a modifying additive are added, and the mixture is homogenized at 8000rpm to obtain a cream base; 0.2kg of chlorhexidine acetate is added to the cream base, and the mixture is mixed evenly to obtain an antibacterial cream.

[0059] The oil phase is composed of beeswax, glyceryl monostearate, liquid paraffin and lanolin in a mass ratio of 2:5:8:5.

[0060] The emulsifier is composed of Span 60 and glyceryl polyethylene glycol-75 stearate in a mass ratio of 2:1.

[0061] The preparation method of the modified additive is as follows:

[0062] S1. Mix 1 kg of hydroxypropyl deacetylated chitosan with 20 kg of water and stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 65° C., and gradually add 3 kg of locust bean gum at a stirring speed of 400 rpm, and continue stirring for 2 hours to form a polysaccharide complex;

[0063] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of acacia aldehyde, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0064] Example 2

[0065] The preparation method of an antibacterial cream containing snail protease and herbal extract is basically the same as that of Example 1, the only difference being that the preparation method of the modified additive is different.

[0066] The preparation method of the modified additive is as follows:

[0067] S1. Mix 1 kg of carboxymethyl chitosan with 20 kg of water and stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 65° C., and gradually add 3 kg of locust bean gum at a stirring speed of 400 rpm, and continue stirring for 2 hours to form a polysaccharide complex;

[0068] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of acacia aldehyde, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0069] The oil phase is the same as in Example 1.

[0070] The emulsifier is the same as that in Example 1.

[0071] Example 3

[0072] The preparation method of an antibacterial cream containing snail protease and herbal extract is basically the same as that of Example 1, the only difference being that the preparation method of the modified additive is different.

[0073] The preparation method of the modified additive is as follows:

[0074] S1. Mix 1 kg of α-cyclodextrin with 20 kg of water and stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 65° C., and gradually add 3 kg of locust bean gum at a stirring speed of 400 rpm, and continue stirring for 2 hours to form a polysaccharide complex;

[0075] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of acacia aldehyde, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0076] The oil phase is the same as in Example 1.

[0077] The emulsifier is the same as that in Example 1.

[0078] Example 4

[0079] The preparation method of an antibacterial cream containing snail protease and herbal extract is basically the same as that of Example 1, the only difference being that the preparation method of the modified additive is different.

[0080] The preparation method of the modified additive is as follows:

[0081] S1. Mix 1 kg of soluble starch with 20 kg of water and stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 65° C., and gradually add 3 kg of locust bean gum at a stirring speed of 400 rpm, and continue stirring for 2 hours to form a polysaccharide complex;

[0082] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of acacia aldehyde, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0083] The oil phase is the same as in Example 1.

[0084] The emulsifier is the same as that in Example 1.

[0085] Example 5

[0086] The preparation method of an antibacterial cream containing snail protease and herbal extract is basically the same as that of Example 1, the only difference being that the preparation method of the modified additive is different.

[0087] The preparation method of the modified additive is as follows:

[0088] S1. Mix 1 kg of hydroxypropyl deacetylated chitosan with 20 kg of water and stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 65° C., and gradually add 3 kg of xanthan gum at a stirring speed of 400 rpm, and continue stirring for 2 hours to form a polysaccharide complex;

[0089] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of acacia aldehyde, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0090] The oil phase is the same as in Example 1.

[0091] The emulsifier is the same as that in Example 1.

[0092] Example 6

[0093] The preparation method of an antibacterial cream containing snail protease and herbal extract is basically the same as that of Example 1, the only difference being that the preparation method of the modified additive is different.

[0094] The preparation method of the modified additive is as follows:

[0095] S1. Mix 1 kg of hydroxypropyl deacetylated chitosan with 20 kg of water and stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 65° C., and gradually add 3 kg of carrageenan at a stirring speed of 400 rpm, and continue stirring for 2 hours to form a polysaccharide complex;

[0096] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of acacia aldehyde, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0097] The oil phase is the same as in Example 1.

[0098] The emulsifier is the same as that in Example 1.

[0099] Example 7

[0100] The preparation method of an antibacterial cream containing snail protease and herbal extract is basically the same as that of Example 1, the only difference being that the preparation method of the modified additive is different.

[0101] The preparation method of the modified additive is as follows:

[0102] S1. Mix 1 kg of hydroxypropyl deacetylated chitosan with 20 kg of water and stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 65° C., and gradually add 3 kg of locust bean gum at a stirring speed of 400 rpm, and continue stirring for 2 hours to form a polysaccharide complex;

[0103] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of 3,7,9-trimethyl-2,6-decadienal, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0104] The oil phase is the same as in Example 1.

[0105] The emulsifier is the same as that in Example 1.

[0106] Comparative Example 1

[0107] The preparation method of an antibacterial cream containing snail protease and herbal extract is basically the same as that of Example 1, the only difference being that the preparation method of the modified additive is different.

[0108] The preparation method of the modified additive is as follows:

[0109] S1. Mix 1 kg of pullulan with 20 kg of water and stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 65° C., and gradually add 3 kg of locust bean gum at a stirring speed of 400 rpm, and continue stirring for 2 hours to form a polysaccharide complex;

[0110] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of acacia aldehyde, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0111] The oil phase is the same as in Example 1.

[0112] The emulsifier is the same as that in Example 1.

[0113] Comparative Example 2

[0114] The preparation method of an antibacterial cream containing snail protease and herbal extract is basically the same as that of Example 1, the only difference being that the preparation method of the modified additive is different.

[0115] The preparation method of the modified additive is as follows:

[0116] S1, 1kg of hydroxypropyl deacetylated chitosan was mixed with 20kg of water, and stirred evenly to form a polysaccharide solution; the polysaccharide solution was heated in a water bath at 65°C, and 3kg of konjac gum was gradually added at a stirring speed of 400rpm, and stirring was continued for 2 hours to form a polysaccharide complex;

[0117] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of acacia aldehyde, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0118] The oil phase is the same as in Example 1.

[0119] The emulsifier is the same as that in Example 1.

[0120] Comparative Example 3

[0121] The preparation method of an antibacterial cream containing snail protease and herbal extract is basically the same as that of Example 1, the only difference being that the preparation method of the modified additive is different.

[0122] The preparation method of the modified additive is as follows:

[0123] S1. Mix 1 kg of hydroxypropyl deacetylated chitosan with 20 kg of water and stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 65° C., and gradually add 3 kg of locust bean gum at a stirring speed of 400 rpm, and continue stirring for 2 hours to form a polysaccharide complex;

[0124] S2. Mix 1.5 kg of ginseng extract with the polysaccharide complex in step S1, add 1.5 kg of citral, adjust the pH value to 6 with 1 mol / L acetic acid aqueous solution, treat at 60° C. for 3 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 600 bar for 5 minutes to obtain a modified additive.

[0125] The oil phase is the same as in Example 1.

[0126] The emulsifier is the same as that in Example 1.

[0127] Comparative Example 4

[0128] A method for preparing an antibacterial cream containing snail protease and herbal extract is as follows:

[0129] 0.8kg of honeysuckle, 1.5kg of cnidium monnieri, 0.5kg of sophora flavescens, 1.5kg of radix stemonae, 2kg of cortex difficile and 1kg of fructus kochiae are decocted and extracted with 60kg of water to obtain an extract; the extract is filtered through a 300-mesh filter cloth and concentrated to a density of 1.28g / mL at 80°C to obtain a herbal extract; 0.4kg of snail protease is added to 2kg of water to obtain a snail protease solution; 1kg of borneol and 0.5kg of menthol are added to 10kg of ethanol to obtain a borneol-menthol solution; the herbal extract, the snail protease solution and the borneol-menthol solution are mixed evenly; 12kg of an oil phase and 2kg of an emulsifier are added, and the mixture is homogenized at 8000rpm to obtain a cream base; 0.2kg of chlorhexidine acetate is added to the cream base, and the mixture is mixed evenly to obtain an antibacterial cream.

[0130] The oil phase is composed of beeswax, glyceryl monostearate, liquid paraffin and lanolin in a mass ratio of 2:5:8:5.

[0131] The emulsifier is composed of Span 60 and glyceryl polyethylene glycol-75 stearate in a mass ratio of 2:1.

[0132] Test Example 1

[0133] Skin irritation test

[0134] The antibacterial cream obtained in the embodiment and the comparative example was subjected to a skin irritation test: 0.5 g of the antibacterial cream was applied to the belly of 55 mice until absorbed, with 5 mice in each group, and observed for three days. The mice showed no abnormalities and no rash allergic characteristics, indicating that the antibacterial cream of the present invention is non-irritating to the skin.

[0135] Test Example 2

[0136] Storage performance test

[0137] The antibacterial creams of the above examples and comparative examples were respectively taken, placed in sealed glass vials, placed in a constant temperature box at 65° C. for 5 days, and their properties and uniformity were observed.

[0138] The test results are shown in Table 1.

[0139] Table 1

[0140]

[0141]

[0142] Test Example 3

[0143] Inhibition zone test

[0144] Pour the agar cooled to 40℃ into the plate and let it stand to condense for later use. Prepare 1×10 Escherichia coli (from China Agricultural Microbiological Culture Collection Administration Center, ACCC 11902) and Staphylococcus aureus (from China General Microbiological Culture Collection Administration Center, CGMCC1.282) respectively. 5 100 μL of bacterial suspension of 100 μL / ml was taken, and 200 μL of the prepared bacterial solution was poured into a plate and evenly coated with a coating rod, and then allowed to stand for 5 minutes to dry naturally at room temperature. 10 μL of the antibacterial cream of the embodiment and the comparative example placed in the incubator of test example 2 for 5 days was dropped into each filter paper sheet, and the side with the drop was evenly placed on the bacterial plate with 3 sheets per plate. After inverted culture at 37°C for 18 hours, the results were observed and the diameter of the antibacterial zone was measured. The test results are shown in Table 2.

[0145] Table 2

[0146]

[0147]

[0148] It can be seen from the data in Tables 1 and 2 that the antibacterial cream prepared in Example 1 has the best storage performance and still has a high antibacterial effect after storage.

[0149] Compared with other polysaccharide materials such as carboxymethyl chitosan, α-cyclodextrin, soluble starch and pullulan, hydroxypropyl chitosan has better storage performance and antibacterial effect after storage, which may be related to its unique molecular structure and chemical properties. Hydroxypropyl chitosan contains -NH2 groups in the molecule, which can combine with free H+ to form -NH 3+ Cations, these cations can combine with negatively charged anions on the bacterial cell wall, destroy the cell wall and cell membrane, inhibit the normal physiological metabolism of bacteria, and thus have an antibacterial effect. In addition, hydroxypropyl chitosan can also achieve the antibacterial purpose by inhibiting DNA transcription in bacterial cells, hindering the translation of mRNA codons, and causing the termination of bacterial reproduction. This antibacterial mechanism is effective under a variety of pH conditions, making hydroxypropyl chitosan have a wide range of antibacterial properties. In contrast, other polysaccharide materials such as carboxymethyl chitosan may only show good water solubility and antibacterial properties at specific pH values, limiting their performance in different application environments. Therefore, due to its excellent antibacterial properties and wide pH stability, hydroxypropyl chitosan has better storage performance and antibacterial effect in the preparation of antibacterial creams.

[0150] The locust bean gum used in Example 1 performs better in storage performance and antibacterial effect after storage than the xanthan gum in Example 5, the carrageenan in Example 6, and the konjac gum in Comparative Example 2, which may be related to the unique properties of locust bean gum. Locust bean gum has good gel properties and stability, which enables it to form a stable structure in the cream, thereby maintaining the uniformity and appearance of the cream during storage.

[0151] The acacia aldehyde used in Example 1 performs better in storage performance and antibacterial effect after storage than 3,7,9-trimethyl-2,6-decadienal in Example 7 and citral in Comparative Example 3, which may be related to the unique chemical properties and biological activity of acacia aldehyde. As a flavonoid compound, acacia aldehyde has good antioxidant and antibacterial properties. In addition, acacia aldehyde has a significant inhibitory effect on the proliferation of tumor cells, and can regulate signal pathways and induce apoptosis of cancer cells. These biological activities may make acacia aldehyde have better stability and antibacterial effect in antibacterial creams. In contrast, although 3,7,9-trimethyl-2,6-decadienal and citral also have certain antibacterial activity, they may not be as good as acacia aldehyde in storage performance and antibacterial effect. As a terpenoid compound, the antibacterial mechanism of 3,7,9-trimethyl-2,6-decadienal may be related to the destruction of the integrity of bacterial cell membranes, while citral inhibits microbial growth by changing cell permeability and causing endolyzed substances to seep out. However, as a flavonoid compound, acaciaaldehyde's wide range of biological activities and strong antioxidant properties may be the reason why it performs better in antibacterial creams.

[0152] There may be an interaction between hydroxypropyl chitosan and locust bean gum, which may be related to the change in the chain structure of the polysaccharide. Hydroxypropyl chitosan is a chemically modified polysaccharide with a specific chemical structure and biological activity. Locust bean gum is also a polysaccharide, and when mixed, they may interact through non-covalent bonds, such as hydrogen bonds, van der Waals forces, etc., to form a complex polysaccharide network structure. These interactions may affect the solubility, viscosity and gelation behavior of polysaccharides, thereby providing better stability and uniformity in the cream. As an active ingredient, acacia aldehyde contributes mainly to the antibacterial effect of the cream through its own antibacterial properties.

Claims

1. A method for preparing an antibacterial cream containing snail protease and herbal extract, characterized in that: The method is as follows, in parts by weight: 0.5-1 part of honeysuckle, 1-2 parts of cnidium monnieri, 0.4-0.6 part of sophora flavescens, 1-2 parts of radix stemonae, 1.5-2.5 parts of cortex difficile, and 0.8-1.2 parts of fructus kochiae are decocted and extracted with 50-70 parts of water to obtain an extract; the extract is filtered and concentrated to obtain a herbal extract; 0.3-0.5 parts of snail protease are added to 1-3 parts of water to obtain a snail protease solution; 0.8-1.2 parts of borneol and 0.4- 0.6 parts of menthol are added to 8-12 parts of ethanol to obtain a borneol-menthol solution; the herbal extract, the snail protease solution and the borneol-menthol solution are mixed evenly; 10-14 parts of an oil phase, 1-3 parts of an emulsifier and 0.8-1.2 parts of a modifying additive are added, and homogenization is performed at 5000-10000 rpm to obtain a cream base; 0.1-0.3 parts of chlorhexidine acetate are added to the cream base, and the mixture is mixed evenly to obtain an antibacterial cream.

2. The method for preparing the antibacterial cream containing snail protease and herbal extract according to claim 1, characterized in that: The extract is filtered through a 200-400 mesh filter cloth.

3. The method for preparing the antibacterial cream containing snail protease and herbal extract according to claim 1, characterized in that: The concentration is to concentrate at 70-90° C. to a density of 1.2-1.3 g / mL.

4. The method for preparing the antibacterial cream containing snail protease and herbal extract according to claim 1, characterized in that: The oil phase is at least one of beeswax, glyceryl monostearate, liquid paraffin and lanolin.

5. The method for preparing the antibacterial cream containing snail protease and herbal extract according to claim 1 or 4, characterized in that: The oil phase is composed of beeswax, glyceryl monostearate, liquid paraffin and lanolin in a mass ratio of 1-3:4-6:7-9:4-6.

6. The method for preparing the antibacterial cream containing snail protease and herbal extract according to claim 1, characterized in that: The emulsifier is at least one of Span 60 and glyceryl macrogol-75 stearate.

7. The method for preparing the antibacterial cream containing snail protease and herbal extract according to claim 1 or 6, characterized in that: The emulsifier is composed of Span 60 and glycerol macrogol-75 stearate in a mass ratio of 1 to 3:

1.

8. The method for preparing the antibacterial cream containing snail protease and herbal extract according to claim 1, characterized in that: The preparation method of the modified additive is as follows, in parts by weight: S1. Mix 0.8-1.2 parts of hydroxypropyl deacetylated chitosan with 15-25 parts of water, stir evenly to form a polysaccharide solution; heat the polysaccharide solution in a water bath at 60-70° C., and gradually add 2-4 parts of locust bean gum at a stirring speed of 300-500 rpm, and continue stirring for 1-3 hours to form a polysaccharide complex; S2. Mix 1 to 2 parts of ginseng extract with the polysaccharide complex in step S1, add 1 to 2 parts of acacia aldehyde, adjust the pH value to 5 to 6.5 with a 0.5 to 2 mol / L acetic acid aqueous solution, treat at a temperature of 50 to 70° C. for 2 to 4 hours to form a mixture, and treat the mixture by a high-pressure homogenization method at a pressure of 400 to 800 bar for 3 to 8 minutes to obtain a modified additive.

9. An antibacterial cream containing snail protease and herbal extract, characterized in that: Prepared by the preparation method according to any one of claims 1 to 8.

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