Skin-friendly fabric capable of continuously releasing vitamin B5, vitamin C and collagen peptide simultaneously, sanitary towel, method and application

Abstract

The invention relates to the technical field of sanitary napkins, in particular to a skin-friendly fabric capable of continuously releasing vitamin B5, vitamin C and collagen peptide at the same time, a sanitary napkin, a method and application. A skin-friendly fabric is obtained by treating a nano slow-release composition loaded with vitamin B5, vitamin C and collagen peptide into a pure cotton fabric, and the skin-friendly fabric is used as an inner layer material to prepare the sanitary towel. The skin-friendly fabric and the sanitary towel have the function of slowly and continuously releasing the vitamin B5, the vitamin C and the collagen peptide at the same time, the protection effect of the vitamin B5, the vitamin C and the collagen peptide on vagina health can be continuously kept, and the vagina health can be maintained for a long time.

Description

Technical Field

[0001] The invention relates to the technical field of sanitary napkins, and in particular to skin-friendly fabrics, sanitary napkins, methods and applications for simultaneously and continuously releasing vitamin B5, vitamin C and collagen peptides. Background Art

[0002] Sanitary napkins are a kind of female hygiene products with good absorption. The main materials are cotton, non-woven fabrics, pulp or polymers and polymer composite paper formed by the above materials. The side design is mainly used to prevent side leakage. The adhesive backing of the sanitary napkin is a waterproof material, which can retain the menstrual blood in the sanitary napkin and absorb the menstrual blood flowing out of the vagina during menstruation. The surface layer of existing sanitary napkins is generally non-woven fabric or cotton-rubber mixed layer, the overall structure cost is low, and the assembly process is also relatively simple. Summary of the invention

[0003] In view of this, the present invention provides a sanitary napkin that simultaneously and continuously releases vitamin B5, vitamin C and collagen peptides and a preparation method thereof. The present invention obtains a skin-friendly fabric by treating a nano slow-release composition loaded with vitamin B5, vitamin C and collagen peptides into pure cotton fabric, and prepares a sanitary napkin using the skin-friendly fabric as an inner layer material. The skin-friendly fabric and the sanitary napkin have the function of simultaneously and slowly and continuously releasing vitamin B5, vitamin C and collagen peptides, and can continuously maintain the protective effects of vitamin B5, vitamin C and collagen peptides on vaginal health, and maintain vaginal health for a long time.

[0004] One of the purposes of the present invention is to provide a method for preparing a skin-friendly fabric, comprising: preparing a nano sustained-release composition, wherein the nano sustained-release composition is calcium carbonate particles encapsulating vitamin B5, vitamin C and collagen peptides; treating pure cotton fabric with glutaraldehyde; and laying the nano sustained-release composition between two layers of pure cotton fabric treated with glutaraldehyde, and obtaining the skin-friendly fabric by pressing.

[0005] Specifically, the steps of preparing the nano sustained-release composition include: adding sodium polyaspartate to a calcium chloride solution, and dripping a sodium carbonate solution after the reaction; adding an inclusion compound solution after the dripping is completed, ultrasonically treating, and continuously adsorbing under stirring; after the adsorption is completed, centrifuging the solution, washing with ethanol, centrifuging, and freeze-drying to constant weight to obtain the nanoparticles; preparing an aqueous solution containing the nanoparticles and reacting it with stearic acid by wet method at room temperature for at least 48 hours, washing with ethanol, centrifuging, and freeze-drying to constant weight to obtain the nano sustained-release composition.

[0006] Specifically, the steps of preparing the nano sustained-release composition specifically include: taking 20 mL of 0.1M sodium carbonate solution and 0.1M calcium chloride solution, adding 666 mg of sodium polyaspartate to the calcium chloride solution and stirring for cross-linking for 30 minutes, and then adding the sodium carbonate solution dropwise to the calcium chloride solution to which the sodium polyaspartate was added at a rate of 10 mL / min under stirring; after the addition is completed, adding 50 mL of a 30 mg / mL vitamin B5 aqueous solution, or an aqueous solution containing 10 mg / mL vitamin C and 30 mg / mL vitamin B5, or an aqueous solution containing 5 mg / mL flaxseed collagen protein; The suspension of white peptide and 10 mg / mL vitamin C and 30 mg / mL vitamin B5 was ultrasonicated for 30 minutes; then the adsorption was continued at a speed of 1000 rpm for 24 hours; after the reaction was completed, the suspension was centrifuged at a speed of 12000 rpm for 30 minutes, washed with ethanol, centrifuged, and freeze-dried to constant weight to obtain nanoparticles; an aqueous solution containing 80 wt% of nanoparticles was mixed with stearic acid, and the mixture was placed at room temperature for 72 hours by a wet method, taken out, centrifuged at a speed of 12000 rpm for 30 minutes, washed with ethanol, centrifuged, and freeze-dried to constant weight to obtain a nano sustained-release composition.

[0007] Specifically, the amount of stearic acid added is 1.0-1.25% of the mass of the nanoparticles.

[0008] One of the purposes of the present invention is to provide a method for preparing the sanitary napkin. The method comprises: preparing a nano slow-release composition, wherein the nano slow-release composition is calcium carbonate particles containing vitamin B5, vitamin C and collagen peptides; treating pure cotton fabric with glutaraldehyde; laying the nano slow-release composition between two layers of pure cotton fabric treated with glutaraldehyde, and obtaining a skin-friendly fabric by pressing; and sewing the skin-friendly fabric or a plurality of the skin-friendly fabrics as the inner layer material into the outer layer material to obtain the sanitary napkin.

[0009] Specifically, the sanitary napkin comprises an inner layer facing the body surface and a liquid-proof outer layer facing the clothing surface; wherein the inner layer is sewn from a skin-friendly fabric containing vitamin B5, and the inner layer and the outer layer are sewn into a whole.

[0010] Specifically, the outer layer extends outward to completely cover the inner layer, and extends outward to have two side wings; wherein the dividing portion between the inner layer and the outer layer is sewn by elastic spandex yarn and divides the inner layer and the side wings of the outer layer, and the inner layer is sewn in the middle position of the outer layer.

[0011] Specifically, elastic spandex yarns are embedded in the edges of the inner layer and the outer layer.

[0012] Specifically, the inner layer includes multiple layers of the skin-friendly fabric.

[0013] Specifically, the method of treating the pure cotton fabric with glutaraldehyde comprises: taking 200 mL of a 0.5 wt % glutaraldehyde aqueous solution with a pH of 5, adding 4 g of magnesium chloride crystals, stirring to completely dissolve the magnesium chloride crystals, and then adding 200 cm 2 High-count gauze, finally put the conical flask into a constant temperature oscillator, treat it in a constant temperature water bath oscillator at 60℃ for 1 hour, take out the high-count gauze, wash it with deionized water or soak it for 3 hours, take it out and press it with a rolling car, the rolling rate is 60%; wash it again with deionized water until no glutaraldehyde is detected in the residual liquid.

[0014] Specifically, the pressing comprises: pressing the nano sustained-release composition at 50 mg / cm 2 The mixture is laid between two layers of pure cotton fabrics treated with glutaraldehyde at a ratio of ; taken out and pressed with a rolling mill, with a rolling rate of 60%; soaked in deionized water again for 3 hours, taken out and pressed with a rolling mill, with a rolling rate of 60%, and dried at room temperature to obtain the skin-friendly fabric.

[0015] One of the purposes of the present invention is to provide a use of a skin-friendly fabric in the preparation of a sanitary napkin.

[0016] Beneficial effects of the present invention: The present invention prepares a nano sustained-release composition containing vitamin B5, vitamin C and collagen peptides, and presses the nano sustained-release composition into treated pure cotton fabrics. Compared with the nano sustained-release composition, the present invention further improves the sustained-release effect, can continuously release vitamin B5, vitamin C and collagen peptides, can continuously maintain the protective effects of vitamin B5, vitamin C and collagen peptides on vaginal health, and maintain vaginal health for a long time.

[0017] The present invention prepares a nano sustained-release composition and presses it into a treated high-count gauze to obtain a skin-friendly fabric with close fitting and comfort. The skin-friendly fabric can be used to prepare an inner layer directly facing the body surface and a sanitary napkin composed of the inner layer, which can not only achieve a close fitting and skin-friendly effect, but also avoid the formation of a gap between the inner layer and the human body when the user is in motion, thereby improving the close fitting of the inner layer and ensuring the absorption effect. In addition, since the skin-friendly fabric has the function of continuously releasing vitamin B5, vitamin C and collagen peptides, the inner layer made of the skin-friendly fabric can not only maintain the integrity of the cell membrane of the vaginal mucosal cells, but also effectively resist the invasion of external pathogens and maintain the balance of the vaginal microbial community.

[0018] The present invention has found through cell experiments that the skin-friendly fabric not only has no obvious toxic side effects on vaginal mucosal epithelial cells, but also can maintain the integrity of the cell membranes of vaginal mucosal cells, effectively resist the invasion of external pathogens, and maintain the balance of vaginal microbial communities.

[0019] The present invention has found through cell experiments that the skin-friendly fabric and the sanitary napkin made based on the fabric can not only activate the NLRP3 inflammasome to a certain extent within 12 hours to initiate an immune response to Candida albicans, but also prevent the lysis of vaginal epithelial cells due to inflammatory response after 48 hours, thereby preventing damage to the vaginal mucosa. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The vitamin B5 loss rate in steps 1) to 3) during the preparation of the skin-friendly fabric 1.

[0021] Figure 2 It is the loss rate of vitamin B5 and vitamin C in steps 1) to 3) during the preparation process of skin-friendly fabric 2.

[0022] Figure 3 The loss rates of vitamin B5, vitamin C and collagen peptides in steps 1) to 3) during the preparation of skin-friendly fabric 3.

[0023] Figure 4 It is the vitamin B5 loss rate of skin-friendly fabrics 1 to 5 during the washing resistance test.

[0024] Figure 5 It is the vitamin C loss rate of skin-friendly fabric 2~5 during the washing resistance test.

[0025] Figure 6 It is the collagen peptide loss rate of skin-friendly fabric 3~5 during the washing resistance test.

[0026] Figure 7 It is the cumulative release rate of vitamin B5 of vitamin B5 powder and sanitary napkins 1 to 5 in vaginal simulated fluid.

[0027] Figure 8 It is the cumulative release rate of vitamin C from vitamin C powder and sanitary napkin 2~5 in vaginal simulated fluid.

[0028] Fig. 9 It is the cumulative release rate of collagen peptides from flaxseed collagen peptide products and sanitary napkins 3~5 in vaginal simulated fluid.

[0029] Fig.10 This is a graph showing the effects of groups 1 to 5 on the activity of primary rabbit vaginal mucosal epithelial cells.

[0030] Fig.11 This is the result graph of LDH release rate of primary rabbit vaginal mucosal epithelial cells in groups 1 to 5.

[0031] Fig.12 The graph shows the 12-hour NLRP3 expression results of primary vaginal mucosal epithelial cells of rabbits treated with Candida albicans in groups 1 to 5.

[0032] Fig.13 The graph shows the 48-hour NLRP3 expression results of primary vaginal mucosal epithelial cells of rabbits treated with Candida albicans in groups 1 to 5.

[0033] Fig.14 This is a schematic diagram of the structure of the sanitary napkin provided by the present invention.

[0034] Fig.15 This is a schematic diagram of the side view of the sanitary napkin provided by the present invention. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical scheme and advantages of the present invention more clear, the present invention is further described in detail below in combination with the groups. It should be understood that the specific groups described herein are only used to explain the present invention and are not used to limit the present invention. The reagents not described in detail in the present invention are all conventional reagents and can be obtained from commercial channels; the methods not described in detail are all conventional experimental methods and can be obtained from the prior art.

[0036] Example 1: Preparation of skin-friendly fabric 1) Preparation of nano sustained-release composition Take 20 mL of 0.1M sodium carbonate solution and 0.1M calcium chloride solution, add 666 mg of sodium polyaspartate to the calcium chloride solution and stir for crosslinking for 30 minutes, then add the sodium carbonate solution to the calcium chloride solution with sodium polyaspartate added at a rate of 10 mL / min under stirring. After the addition is completed, add 50 mL of 30 mg / mL vitamin B5 aqueous solution and ultrasonicate for 30 minutes, then continue to adsorb at a speed of 1000 rpm for 24 hours. After the reaction is completed, centrifuge the suspension at a speed of 12000 rpm for 30 minutes, wash with ethanol, centrifuge, and freeze-dry to constant weight to obtain nanoparticles; An aqueous solution containing 80 wt% of nanoparticles was prepared, and then 1.25% stearic acid was added based on the mass of the nanoparticles. The solution was placed at room temperature for 72 h by wet method, then taken out and centrifuged at 12,000 rpm for 30 min. After washing with ethanol and centrifuging, the solution was freeze-dried to constant weight to obtain a nano sustained-release composition.

[0037] The nanometer laser particle size analyzer was used to measure the average particle size of the nanometer sustained-release composition in the suspension after the reaction, which was 369 nm and the polydispersity index was 0.142.

[0038] 2) Treating pure cotton fabric with glutaraldehyde Take 200 mL of 0.5 wt % glutaraldehyde aqueous solution with a pH of 5, add 4 g of magnesium chloride crystals, stir until it is completely dissolved, and then add 200 cm 2High-count gauze (80S, density specification 120×80), after being treated in a constant temperature water bath oscillator at 60℃ for 1 hour, the high-count gauze is taken out, washed with deionized water or soaked for 3 hours, and then taken out and rolled with a rolling car, with a rolling rate of 60%. It is OK to wash it with deionized water again until no glutaraldehyde is detected in the residual liquid.

[0039] 3) Pressing The nano sustained-release composition obtained in step 1) was added at 50 mg / cm 2 The ratio of 20% to 20% was laid between the two layers of pure cotton fabric obtained in step 2). The fabric was taken out and pressed with a rolling mill, with a rolling rate of 60%. The fabric was soaked in deionized water for 3 hours, taken out and pressed with a rolling mill, with a rolling rate of 60%, and dried at room temperature to obtain skin-friendly fabric 1.

[0040] Furthermore, in the above step 1), a nano sustained-release composition is prepared using an aqueous solution containing 10 mg / mL vitamin C and 30 mg / mL vitamin B5, and the other steps are the same as those in the above embodiment to prepare a skin-friendly fabric 2.

[0041] Furthermore, in the above step 1), a suspension containing 5 mg / mL flaxseed collagen peptide (Jinmu Group), 10 mg / mL vitamin C and 30 mg / mL vitamin B5 was prepared, and a nano sustained-release composition was prepared after ultrasonic re-dispersion. The other steps were the same as the above embodiment to prepare skin-friendly fabric 3.

[0042] The loss rates of vitamin B5, vitamin C and collagen peptides in each step are calculated. The loss rate of vitamin B5 in step 1) is the percentage of the weight of vitamin B5 in the nano sustained-release composition suspension to the weight of the total vitamin B5 input. The loss rate of vitamin B5 in step 2) is the percentage of the weight of vitamin B5 in the aqueous solution obtained by washing to the weight of the total vitamin B5 input. The loss rate of vitamin B5 in step 3) is the percentage of the weight of vitamin B5 in the aqueous solution obtained by elution to the weight of the total vitamin B5 input.

[0043] like Figure 1 As shown, the loss rate of vitamin B5 in step 1) to step 3) of the preparation process of the skin-friendly fabric 1 decreases successively, and finally the nano sustained-release composition is stably pressed between the pure cotton fabrics, thereby preparing the skin-friendly fabric 1.

[0044] like Figure 2 As shown, the loss rates of vitamin B5 and vitamin C in step 1) to step 3) of the preparation process of the skin-friendly fabric 2 decrease successively, and finally the nano sustained-release composition is stably pressed between the pure cotton fabrics, thereby obtaining the skin-friendly fabric 2.

[0045] like Figure 3As shown, the loss rates of vitamin B5, vitamin C and collagen peptides in step 1) to step 3) of the preparation process of skin-friendly fabric 3 decrease successively. Although the loss of collagen peptides in step 1) is relatively large, the nano sustained-release composition is finally stably pressed between the pure cotton fabrics, thereby obtaining skin-friendly fabric 3.

[0046] Comparative Example 1: Preparation of skin-friendly fabric Referring to CN112334101A, a mixture of pulp fiber and highly absorbent polymer (weight per unit area: 200 g / m 2 The absorbent body is made of a material composed of a pulp fiber and a super absorbent polymer with a mass ratio of 10:1 and a thickness of 2.0 mm, and a suspension containing 5 mg / mL flaxseed collagen peptide (Jinmu Group), 10 mg / mL vitamin C and 30 mg / mL vitamin B5 at a temperature of 50 mg / cm 2 The absorbent is coated with a coating amount, and the two layers of the absorbent are pressed together with a rolling rate of 60%, and dried at room temperature to prepare a skin-friendly fabric 4.

[0047] Comparative Example 2: Preparation of skin-friendly fabric Referring to CN112334101A, a mixture of pulp fiber and highly absorbent polymer (weight per unit area: 200 g / m 2 The absorbent is formed by a material with a mass ratio of pulp fiber to super absorbent polymer of 10:1 and a thickness of 2.0 mm, and the same mass of flaxseed collagen peptide, vitamin C and vitamin B5 as that contained in skin-friendly fabric 3 are pressed between the two layers of absorbent. The skin-friendly fabric 5 is taken out and pressed with a rolling machine, with a rolling rate of 60%, and dried at room temperature to obtain skin-friendly fabric 5.

[0048] Test Example 1: Washability test of skin-friendly fabrics The skin-friendly fabrics 1 to 5 prepared in the above Example 1, Comparative Example 1 and Comparative Example 2 were subjected to a washing resistance test using a household washing machine, and the washing procedure was 4N, i.e., washing at 40°C for 15 min and hanging to dry. Referring to the above method, the loss rates of vitamin B5, vitamin C and collagen peptides of the skin-friendly fabrics 1 to 5 before and after washing were calculated.

[0049] like Figure 4 As shown in the figure, the loss rate of vitamin B5 in skin-friendly fabrics 1 to 3 is significantly lower than that in skin-friendly fabrics 4 and 5. Figure 5 As shown in the figure, the loss rate of vitamin C in skin-friendly fabrics 2 to 3 is significantly lower than that in skin-friendly fabrics 4 and 5. Figure 6 As shown, the loss rate of collagen peptides of skin-friendly fabric 3 is significantly lower than that of skin-friendly fabrics 4 and 5.

[0050] This shows that the skin-friendly fabrics 1 to 3 prepared by the method provided by the present invention can form a deep binding effect between vitamin B5, vitamin C and collagen peptides and pure cotton fabrics, such as forming a covalent bond binding effect with the cellulose surface, so that vitamin B5, vitamin C and collagen peptides will not be easily released from the surface of pure cotton fabrics. Compared with the ordinary coating method of comparative examples 1 and 2, the binding is tighter and the water washing resistance is stronger, providing a basis for its repeated use. In addition, since it forms a deep binding effect with pure cotton fabrics, it provides a basis for its preparation into sanitary napkins to continuously release and produce biological activity in an environment containing secretions and excrement at the vaginal opening.

[0051] Example 2: Preparation of sanitary napkin See also Fig.14 , Fig.15 A sanitary napkin of a specific embodiment comprises an inner layer 12 facing the body surface and an outer layer 11 facing the clothing surface and preventing liquid from seeping. The inner layer 12 is sewn from the skin-friendly fabric provided in the above embodiment 1. The inner layer 12 and the outer layer 11 are sewn into a whole.

[0052] The outer layer 11 extends outward to completely cover the inner layer 12, and extends outward to have two side wings 120 to prevent side leakage. The inner layer 12 is entirely sewn with skin-friendly fabric. The inner layer 12 and the outer layer are separated by elastic spandex yarn 113 and the side wings 120 of the inner layer 12 and the outer layer 11 are sewn. The inner layer 12 is roughly sewn in the middle of the outer layer 11. Furthermore, the edges of the inner layer and the outer layer are both embedded with elastic spandex yarn.

[0053] In a preferred embodiment, Fig.14 The inner layer 12 includes multiple layers of the skin-friendly fabrics 1 to 5 provided in the above-mentioned embodiment 1, for example, 2 to 5 layers, and the inner layer 12 can be formed by further pressing or sewing.

[0054] The sanitary napkin designed in this way can not only play a close-fitting and skin-friendly role, but also avoid the formation of gaps between the inner layer 12 and the human body when the user is in motion, thereby improving the fit of the inner layer 12 and ensuring the absorption effect.

[0055] During implementation, the outer layer 11 can be made of waterproof and breathable micro-nano fiber non-woven membranes and ordinary PP non-woven composites, polyethylene non-woven fabrics, etc. The lower surface of the outer layer 11 can be provided with a self-adhesive layer to facilitate bonding and fixing with the inner side of women's underwear; the upper surface layer 114 of the outer layer 11 can be made of skin-friendly PP material skin-friendly non-woven fabrics or skin-friendly cotton fabrics made of pure cotton. The lower surface of the outer layer 11 can be made of skin-friendly non-woven fabrics, skin-friendly cotton fabrics made of pure cotton, micro-nano fiber non-woven membranes and ordinary PP non-woven composites, etc. The four edges of the upper and lower surface layers are fixedly connected by high-temperature pressing. Spandex yarn 113 is known as the second skin of the human body. It is safe and comfortable to use and has good elasticity. It can also be replaced by other skin-friendly elastic flexible lines during manufacturing. When the four edges of the upper and lower surface layers are pressed and connected, they are embedded inside the corresponding edges.

[0056] Example 3: Simulation test of sanitary napkin Vaginal simulation liquid was prepared according to the method of "Owen DH, Katz DF. Avaginal fluid stimulant [J]. Contraception, 1999, 59 (2): 91-95", and sanitary napkins 1 to 5 were prepared respectively using the skin-friendly fabrics 1 to 5 prepared in the above Example 1 as raw materials. The sanitary napkins 1 to 5 were completely immersed in the vaginal simulation liquid for 24 hours, and the contents of vitamin B5, vitamin C and collagen peptides in the solution after immersion were detected by conventional methods. The cumulative release rate of vitamin B5, vitamin C and collagen peptides in sanitary napkins 1 to 5 was calculated. The cumulative release rate is the percentage of the mass of vitamin B5, vitamin C and collagen peptides in the solution after immersion to the total weight of sanitary napkins 1 to 5. In addition, the same mass of vitamin B5 powder was directly put into the vaginal simulation liquid to test the cumulative release rate in the solution. In addition, the same mass of vitamin C powder was directly put into the vaginal simulation liquid to test the cumulative release rate in the solution. In addition, the flaxseed collagen peptide product was directly put into the vaginal simulation liquid to test the cumulative release rate in the solution.

[0057] like Figure 7 As shown in the figure, the cumulative release rate of vitamin B5 of sanitary napkins 4 and 5 in the first 14 hours was significantly higher than that of sanitary napkins 1 to 3. Figure 8 As shown, the cumulative release rate of vitamin C from sanitary napkins 4 and 5 was significantly higher than that from sanitary napkins 2 and 3 in the first 18 hours. Fig. 9 As shown, the cumulative release rates of collagen peptides from sanitary napkins 4 and 5 were significantly higher than those from sanitary napkins 2 and 3 in the first 16 hours.

[0058] This shows that the sanitary napkins 1 to 3 prepared by the present invention are respectively prepared by using skin-friendly fabrics 1 to 3, and the skin-friendly fabrics 1 to 3 can make vitamin B5, vitamin C and collagen peptides form a deep binding effect with the pure cotton fabric, so that vitamin B5, vitamin C and collagen peptides will not be easily released from the surface of the pure cotton fabric, and their sustained release effect in the vaginal simulated fluid is obvious. However, the skin-friendly fabrics 4 and 5 only adopt the ordinary coating method, and their sustained release effect is not as good as the sanitary napkins 1 to 3 prepared by the skin-friendly fabrics 1 to 3 provided by the present invention.

[0059] Example 4: Effect of skin-friendly fabrics on vaginal epithelial cells 1. Cell lines and cell culture media Rabbit primary vaginal epithelial cells (Bio-137458, purchased from Beijing Bio-Bowei Biotechnology) were cultured in keratinocyte culture medium (Gibco, USA) at 37°C and 5% CO 2 Under the above conditions, cells grew as monolayer adherently, and cells in logarithmic phase were used for the experiment.

[0060] 2. Effects on cell activity Rabbit primary vaginal mucosal epithelial cells in the logarithmic growth phase were collected and inoculated into 96-well cell culture plates, with about 5000 cells inoculated into each well. After the cells adhered to the wall, the culture medium was aspirated, and the cells were washed once with PBS and divided into groups 1, 2, 3, 4, and 5.

[0061] After adding skin-friendly fabric 3 with a diameter of 3 mm to group 1, 200 μL of culture medium was added and cultured for 24 h. 20 μL of MTT working solution (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide working solution) was added and cultured for 4 h. The mixture was centrifuged and the supernatant was discarded. 150 μL of dimethyl sulfoxide was added to dissolve the purple crystals and the mixture was detected with an enzyme-labeled instrument and the absorbance was measured at 490 nm.

[0062] After adding the same mass of the nano sustained-release composition as that contained in the skin-friendly fabric 3 with a diameter of 3 mm into group 2, the testing steps were the same as those of group 1.

[0063] After adding skin-friendly fabric 4 with a diameter of 3 mm to group 3, the testing steps are the same as group 1.

[0064] The same mass of vitamin C, vitamin B5 and collagen peptide as those contained in the skin-friendly fabric 4 with a diameter of 3 mm were added to group 4, and the testing steps were the same as those of group 1.

[0065] After adding skin-friendly fabric 5 with a diameter of 3 mm to group 5, the testing steps were the same as group 1.

[0066] In addition, a control group was set up without adding skin-friendly fabric or nano sustained-release composition, and the absorbance was measured at 490nm. The percentage of the absorbance difference of each group at 490nm to the control group was calculated, which was the cell survival rate.

[0067] Depend on Fig.10 It can be seen that skin-friendly fabrics 1 to 5 and the nano sustained-release compositions of each group have no obvious toxic side effects on rabbit primary vaginal mucosal epithelial cells.

[0068] 3. Effect on cell LDH (lactate dehydrogenase) release Take 120 μL of the supernatant after 4 hours of treatment of the above-mentioned cells, add it to the corresponding wells of a new 96-well plate, add 60 μL LDH working solution (Lactate dehydrogenase cytotoxicity detection kit, Beyotime Biotechnology Research Institute), protect from light, incubate at room temperature for 0.5 h, detect with an enzyme reader, measure the absorbance at 490 nm, and calculate the percentage of the absorbance difference at 490 nm of each group to that of the control group, which is the cellular LDH release rate.

[0069] Depend on Fig.11 It can be seen that the LDH release rate of each group is relatively low, especially group 1 and group 2. Under normal circumstances, LDH is present in the cytoplasm and cannot penetrate the cell membrane. When the cell is damaged, the cell membrane permeability changes, and LDH can be released from the cell to the extracellular. It is thus shown that the LDH release rate of the skin-friendly fabric, nano slow-release composition, vitamin C, vitamin B5 and collagen peptide provided by the present invention is relatively low, indicating that it acts on the primary vaginal mucosal epithelial cells of rabbits, and does not cause the permeability of its cell membrane to change, that is, there is no obvious toxic and side effect on the primary vaginal mucosal epithelial cells of rabbits, and it is not only possible to maintain the integrity of the cell membrane of the vaginal mucosal cells, but also effectively resist the invasion of external pathogens and maintain the balance of the vaginal microbial community.

[0070] Example 5: NLRP3 inflammasome (NOD-like receptor protein 3 (NLRP3) inflammasome) regulation of skin-friendly fabrics 1. Co-culture of vaginal epithelial cells and Candida albicans Rabbit primary vaginal epithelial cells (Bio-137458, purchased from Beijing Bio-Bowei Biotechnology) were cultured in keratinocyte medium (Gibco, USA) at 37°C and 5% CO 2 Under the conditions, the cells grew in a monolayer adherent to the wall. The logarithmic phase cells were inoculated into a 96-well cell culture plate, with about 5000 cells inoculated into each well. After the cells adhered to the wall, the culture medium was aspirated and the cells were washed once with PBS. There were blank group, model group, group 1, 2, 3, 4, and 5.

[0071] The model group was added with 200 μL of K-SFM culture medium (keratinocyte serum-free culture medium) at 6, 12, 24, and 48 h of culture. 6 CFU / mL of Candida albicans ( Candida albicans , CMCC(F)98001, CMCC), 37℃, 5%CO 2The cells were cultured in an incubator. After 48 hours, the supernatant was collected and stored in a -80°C refrigerator away from light. The cells in the 24-well plate were washed with PBS, fixed with 4% paraformaldehyde, and stored in a 4°C refrigerator.

[0072] After adding skin-friendly fabric 3 with a diameter of 3 mm to group 1, 200 μL of 1×10 6 CFU / mL of Candida albicans, 37°C, 5% CO 2 The cells were cultured in an incubator and other steps were the same as those of the model group.

[0073] After adding the same mass of the nano sustained-release composition contained in the skin-friendly fabric 3 with a diameter of 3 mm to group 2, 200 μL of 1×10 6 CFU / mL of Candida albicans, 37°C, 5% CO 2 The cells were cultured in an incubator and other steps were the same as those of the model group.

[0074] After adding skin-friendly fabric 4 with a diameter of 3 mm to group 3, 200 μL of 1×10 6 CFU / mL of Candida albicans, 37°C, 5% CO 2 The cells were cultured in an incubator and other steps were the same as those of the model group.

[0075] After adding the same mass of vitamin C, vitamin B5 and collagen peptides as those in the skin-friendly fabric 4 with a diameter of 3 mm, 200 μL of 1×10 6 CFU / mL of Candida albicans, 37°C, 5% CO 2 The cells were cultured in an incubator and other steps were the same as those of the model group.

[0076] After adding the skin-friendly fabric 5 with a diameter of 3 mm, 200 μL of 1×10 6 CFU / mL of Candida albicans, 37°C, 5% CO 2 The cells were cultured in an incubator and other steps were the same as those of the model group.

[0077] The blank group was not added with fabric samples and Candida albicans.

[0078] 2. Detection of NLRP3 inflammasome expression Immunofluorescence detection of NLRP3 inflammasome expression in vaginal epithelial cells Cells fixed with 4% paraformaldehyde were fixed with citrate repair solution, permeabilized, washed with PBS, blocked with 5% bovine serum albumin at 37°C, and then NLRP3 inflammasome antibody (1:100 dilution, AG-44B-0008, AdipoGen ® ) 4 ℃ refrigerator overnight, after rewarming, wash with PBS, add diluted fluorescent secondary antibody (1:500 dilution, Invitrogen) according to the instructions, incubate at 37 ℃, add DAPI (4',6-diamidino-2-phenylindole, Invitrogen) for 9 minutes, wash with PBS and observe under fluorescence microscope and take pictures. 7-8 images of each group were selected for fluorescence quantification using ImageProPlus software, and fluorescence was normalized according to the number of cell nuclei before statistical analysis.

[0079] As an important member of the innate immune system, the NLRP3 inflammasome can immediately initiate a protective adaptive immune response to resist pathogens in the early stages of infection. However, if the NLRP3 inflammasome is overactivated, on the one hand, the inflammatory cytokines and chemokines triggered not only fail to fully kill fungi and reduce damage to vaginal epithelial cells, but instead put the vaginal epithelium in a highly inflammatory state, leading to immunopathological inflammation. For example, Candida albicans in the model group causes pathological inflammation to vaginal epithelial cells and accelerates their lysis, which can easily cause damage to the vaginal mucosa and cause pathological damage to the vaginal environment in vivo.

[0080] like Fig.12 and Fig.13 As shown in the figure, after the vaginal epithelial cells in the model group were co-cultured with Candida albicans, the level of NLRP3 inflammasome expressed by the vaginal epithelial cells began to increase from 6 hours, reached a peak at 12 hours, and then gradually decreased to 24 hours later. The vaginal epithelial cells began to lyse, and cell rupture and death could be seen at 48 hours. The fluorescence weakened accordingly, while in the blank group, the cell morphology was intact from beginning to end, and the fluorescence remained relatively stable.

[0081] like Fig.12 As shown in the figure, at 12 h of culture, the normalized fluorescence intensity values ​​of group 15 were significantly lower than those of the model group, and the normalized fluorescence intensity values ​​of group 1 were not significantly different from those of the blank group. Fig.13 As shown, after 48 h of culture, the normalized fluorescence intensity value of the model group was significantly lower than that of the blank group, indicating that the vaginal epithelial cells had been lysed, while the normalized fluorescence intensity value of group 1 was still significantly higher than that of the model group, and the relative expression level of group 1 was correspondingly higher than that of groups 2-5.

[0082] This shows that the skin-friendly fabric 3 provided by the present invention and the sanitary napkin made based on the skin-friendly fabric 3 can not only activate the NLRP3 inflammasome to a certain extent within 12 hours to initiate an immune response to Candida albicans, but also prevent the lysis of vaginal epithelial cells due to inflammatory response after 48 hours, thereby preventing damage to the vaginal mucosa.

[0083] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by any technician familiar with the technical field within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention.

Claims

1. A method for preparing a skin-friendly fabric, characterized in that: include: preparing a nano sustained-release composition, wherein the nano sustained-release composition is calcium carbonate particles encapsulating vitamin B5, vitamin C and collagen peptide; Treating pure cotton fabric with glutaraldehyde; as well as The nano sustained-release composition is laid between two layers of pure cotton fabrics treated with glutaraldehyde, and the skin-friendly fabric is obtained by pressing.

2. The preparation method according to claim 1, characterized in that: The steps of preparing the nano sustained-release composition include: Add sodium polyaspartate to the calcium chloride solution, and after the reaction, dropwise add sodium carbonate solution; After the dropwise addition is completed, the inclusion compound solution is added, ultrasonic treatment is performed, and adsorption treatment is continued under stirring; After the adsorption is completed, the solution is centrifuged, washed with ethanol, centrifuged, and freeze-dried to a constant weight to obtain the nanoparticles; The aqueous solution containing the nanoparticles is prepared and reacted with stearic acid in a wet method at room temperature for at least 48 hours, and then washed with ethanol, centrifuged, and freeze-dried to a constant weight to obtain the nano sustained-release composition.

3. The preparation method according to claim 1, characterized in that: The steps of preparing the nano sustained-release composition specifically include: Take 20 mL of 0.1 M sodium carbonate solution and 20 mL of 0.1 M calcium chloride solution, add 666 mg of sodium polyaspartate to the calcium chloride solution and stir for crosslinking for 30 min, then add the sodium carbonate solution dropwise to the calcium chloride solution with sodium polyaspartate added at a rate of 10 mL / min under stirring; After the dropwise addition, 50 mL of a 30 mg / mL vitamin B5 aqueous solution, or an aqueous solution containing 10 mg / mL vitamin C and 30 mg / mL vitamin B5, or a suspension containing 5 mg / mL flaxseed collagen peptide, 10 mg / mL vitamin C and 30 mg / mL vitamin B5 was added and ultrasonicated for 30 min; Then the adsorption was continued for 24 h at a rotation speed of 1000 rpm; After the reaction, the suspension was centrifuged at 12000 rpm for 30 min, washed with ethanol, centrifuged, and freeze-dried to constant weight to obtain nanoparticles; After mixing an aqueous solution containing 80 wt% of nanoparticles with stearic acid, the mixture was placed at room temperature for 72 hours by a wet method, taken out, centrifuged at 12000 rpm for 30 minutes, washed with ethanol, centrifuged, and freeze-dried to constant weight to obtain a nano sustained-release composition.

4. The preparation method according to claim 3, characterized in that: The amount of stearic acid added is 1.0-1.25% of the mass of the nanoparticles.

5. A method for preparing a sanitary napkin, characterized in that: The sanitary napkin comprises an inner layer facing the body surface and an outer layer facing the clothing surface that is liquid-proof; wherein the inner layer is sewn from a skin-friendly fabric containing vitamin B5, vitamin C and collagen peptides, and the inner layer and the outer layer are sewn into a whole; the outer layer extends outward to completely cover the inner layer, and extends outward to have two side wings; wherein the dividing portion between the inner layer and the outer layer is sewn from elastic spandex yarns that divide the side wings of the inner layer and the outer layer, and the inner layer is sewn in the middle of the outer layer; the edges of the inner layer and the outer layer are both embedded with elastic spandex yarns; the inner layer comprises multiple layers of the skin-friendly fabric; The preparation method comprises: preparing a nano sustained-release composition, wherein the nano sustained-release composition is calcium carbonate particles encapsulating vitamin B5, vitamin C and collagen peptide; Treating pure cotton fabric with glutaraldehyde; Laying the nano sustained-release composition between two layers of pure cotton fabric treated with glutaraldehyde, and pressing to obtain a skin-friendly fabric; The skin-friendly fabric or a plurality of the skin-friendly fabrics are sewn into the outer layer material as the inner layer material to obtain the sanitary napkin.

6. The preparation method according to claim 5, characterized in that: The method of treating the pure cotton fabric with glutaraldehyde specifically comprises: Take 200 mL of 0.5 wt % glutaraldehyde aqueous solution with a pH of 5, add 4 g of magnesium chloride crystals, stir until it is completely dissolved, and then add 200 cm 2 The high-count gauze was treated in a constant temperature water bath oscillator at 60°C for 1 hour, then taken out and washed with deionized water or soaked for 3 hours, then taken out and rolled with a rolling car, with a rolling rate of 60%. No glutaraldehyde was detected in the residual liquid after washing with deionized water again.

7. The preparation method according to claim 5, characterized in that: The rolling specifically comprises: The nano sustained-release composition was added at 50 mg / cm 2 The ratio was laid between two layers of pure cotton fabric treated with glutaraldehyde; The product is taken out and rolled with a rolling machine, and the rolling rate is 60%; The mixture was immersed in deionized water for 3 hours again, taken out and rolled with a rolling machine, with a rolling rate of 60%, and dried at room temperature to obtain the skin-friendly fabric.

8. Use of a skin-friendly fabric in preparing a sanitary napkin, characterized in that: The skin-friendly fabric is prepared by the preparation method according to any one of claims 1 to 4.

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