Triglyceride separation device based on membrane filtration technology

Abstract

The invention relates to a triglyceride separation device based on a membrane filtration technology, in particular to a device applied to separation of triglyceride, protein and the like in serum. The device effectively removes triglyceride particles in serum without influencing the detection of other biochemical components (such as various proteins and the like) by designing a membrane with a proper aperture and combining a centrifugal separation technology. The membrane is made of polyether sulfone (PES) or polytetrafluoroethylene (PTFE) and other materials with good chemical stability and biocompatibility, the pore size of the membrane is designed between 30 nanometers and 80 nanometers, and triglyceride particles can be effectively separated. The device is simple, convenient, low in cost and suitable for rapid treatment of lipid turbidity samples in medical examination, the problem of lipid turbidity interference in the prior art is solved, and the accuracy of biochemical detection such as immunoturbidimetry is improved.

Description

1. Technical field:

[0001] The present invention relates to the field of medical testing, in particular to a triglyceride separation device based on membrane filtration technology, which is suitable for separating triglycerides and other biochemical components (such as various proteins, etc.) by centrifugation to improve the accuracy of biochemical testing of serum samples. 2. Background technology:

[0002] When the triglyceride content in serum samples is too high, it will cause serum lipid turbidity, which usually interferes with biochemical detection methods such as immunoturbidimetry, resulting in inaccurate test results. Currently, existing solutions often rely on the natural stratification of serum after high-speed centrifugation and the removal of the clear liquid for testing, but this method is not effective in practical applications. Either it is difficult to achieve obvious stratification, or the upper layer of fat components are easily mixed in when the lower clear liquid is drawn, and it is difficult to eliminate the interference of fat components with immunoturbidimetry detection. Therefore, how to simply and effectively remove triglycerides from serum without affecting other biochemical components is a technical problem that needs to be solved in the field of medical testing. 3. Summary of the invention:

[0003] 3.1 Technical issues to be solved:

[0004] The purpose of the present invention is to provide a new triglyceride separation device, which can effectively remove triglycerides in serum through membrane filtration technology, avoid the interference of triglycerides on biochemical detection, and do not affect the detection results of other biochemical components (such as various proteins, etc.). The device is easy to operate, low in cost, suitable for large-scale application, and solves the interference of serum lipid turbidity on immunoturbidimetry detection.

[0005] 3.2 Technical solution:

[0006] In order to solve the above technical problems, the present invention provides a triglyceride separation device based on membrane filtration technology. The device mainly comprises:

[0007] 1. Membrane filtration device: used to carry the serum sample to be separated. The bottom of the device is suspended to avoid contact with the bottom of the blood collection vacuum tube.

[0008] 2. Membrane material: The membrane material is fixed at the bottom of the device and has a pore size ranging from 30-80 nanometers. It is used to block triglyceride particles and allow proteins and other small molecules to pass through.

[0009] 3. Centrifugal device: Through high-speed centrifugation, the membrane effectively prevents lipid particles from entering the lower liquid.

[0010] 4. Operation steps: Pour the serum into the membrane filtration device, put it into the blood collection vacuum tube, and centrifuge it through a centrifuge to finally obtain the serum with triglyceride removed.

[0011] This technical solution ensures the separation of triglyceride particles from small molecular components such as proteins by rationally selecting membrane pore size and materials, thus guaranteeing the high efficiency and accuracy of the separation effect. 4. Description of the drawings:

[0012] 1. Figure 1 : Schematic diagram of the membrane filtration device after being loaded into the blood collection tube and subjected to high-speed centrifugation.

[0013] 2. Figure 2 : Schematic diagram of the structure of the membrane filtration device.

[0014] In the figure: 1. Blood collection tube 2. Membrane filtration device 3. Blood collection tube cover 4. Triglyceride molecules simulated by filtration

[0015] 5.Separated serum 6.Filter membrane 5. Specific implementation method:

[0016] 4.1 Membrane design:

[0017] The membrane material is polyethersulfone (PES) or polytetrafluoroethylene (PTFE), which has good chemical stability, anti-lipid pollution ability and biocompatibility. The membrane pore size is designed to be between 30-80 nanometers, which can effectively prevent larger triglyceride particles from passing through while allowing smaller protein molecules to pass through.

[0018] 4.2 Design of membrane filtration device:

[0019] like Figure 1 The volume of the membrane filtration device (2) is designed to be 2-4 ml, the length is about half of the standard blood collection tube (1), and the outer diameter is adapted to be placed inside the standard blood collection tube (1). The bottom of the device is suspended in the air, keeping a distance from the bottom of the blood collection vacuum tube to prevent the membrane from contacting the liquid (5) obtained after filtering at the bottom. The membrane is fixed at the bottom of the device to ensure the stability of the separation effect during centrifugation.

[0020] 4.3 Centrifugation operation:

[0021] ·like Figure 1 , place the membrane filter device into the serum sampling tube, cover it, set the centrifuge speed to 4000rpm, and centrifuge for 5-10 minutes. During the centrifugation process, the fat particles (4) are large in size, and the membrane will effectively prevent these lipid particles from entering the lower liquid (5).

[0022] 4.4 Experimental verification:

[0023] Experimental verification was conducted to determine the concentration of triglycerides in serum samples by colorimetry, and to compare serum samples before and after centrifugation to confirm whether triglycerides were successfully removed. At the same time, other biochemical items such as protein in serum were tested to ensure that the membrane had no adverse effects on other items.

Claims

1. A triglyceride separation device based on membrane filtration technology, comprising a small test tube container, a membrane material and a centrifugal device, wherein the membrane material is used to separate triglyceride particles in serum.

2. The separation device according to claim 1, wherein the membrane pore size is designed to be between 30-80 nanometers, so as to effectively block triglyceride particles and allow protein molecules to pass through.

3. The separation device according to claim 1, wherein the membrane material is polyethersulfone (PES) or polytetrafluoroethylene (PTFE) material, which has excellent anti-lipid pollution performance and biocompatibility.

4. The separation device according to claim 1, wherein the bottom of the small test tube container is suspended in the air and does not contact the bottom of the blood collection vacuum tube to ensure effective separation of the membrane and the bottom liquid.

5. The separation device according to claim 1 further comprises a centrifuge, wherein during the centrifugal operation, the membrane effectively prevents lipid particles from entering the lower liquid.

6. The separation device according to claim 1, wherein after the serum sample is separated by the device, the serum liquid obtained does not contain triglycerides, while other biochemical components (such as various proteins, etc.) are not affected.

7. The separation device according to claim 1, wherein the pore size of the membrane material can simultaneously optimize the separation effect of triglycerides and proteins, etc., to ensure accurate determination of other biochemical items.

8. The separation device according to claim 1, wherein the centrifugal operation is performed at a centrifugal speed of 4000 rpm and a centrifugal time of 5 to 10 minutes.

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