Biological tissue optical characteristic parameter automatic detection device based on single integrating sphere

Abstract

The invention discloses a biological tissue optical characteristic parameter automatic detection device based on a single integrating sphere, and relates to the technical field of biological tissue optical characteristic parameter measurement, the biological tissue optical characteristic parameter automatic detection device comprises a light source module, a sample conveying module, an integrating sphere module, a front end cover shading module and a rear end cover shading module, a light source module is arranged at a position close to one side of a front end hole of the integrating sphere, a rear end cover shading module is arranged at a corresponding position close to one side of a rear end hole of the integrating sphere, a detection optical fiber is electrically connected to an upper hole of the integrating sphere, one end of the detection optical fiber is electrically connected with a spectrograph, and the other end of the detection optical fiber is electrically connected with the spectrograph. The motion control box and the spectrograph are both in control connection with the upper computer PC. By using the rotating platform and the automatic sample clamping device, under the condition that only one integrating sphere is needed, the reflectivity, transmittance and collimation transmittance spectrums of the sample and the reference spectrum of the sample are collected, the detection precision of sample tissue optical parameters is improved, and the detection efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of measuring optical characteristic parameters of biological tissues, in particular to an automatic detection device for optical characteristic parameters of biological tissues based on a single integrating sphere. Background Art

[0002] The accurate and effective measurement of the optical characteristic parameters of biological tissues is of great significance for clarifying the laws of light transmission in biological tissues and exploring new optical detection methods. The measurement methods of the optical characteristic parameters of biological tissues include direct measurement and indirect measurement. The integrating sphere method is one of the commonly used indirect measurement methods. The single integrating sphere technology measures the total reflectance and total transmittance of tissue samples through a single integrating sphere measurement system, and the absorption coefficient μ can be inverted and calculated through the inverse adding-doubling algorithm (IAD). a and the reduced scattering system μ' s However, during the measurement process, the position of the sample needs to be manually changed multiple times to collect the reference spectrum, dark spectrum, sample total reflectance spectrum and total transmittance spectrum. This is not only complicated and cumbersome, but also inefficient. It is also easy to cause the sample to oxidize and deteriorate in the air, and even trigger the light path light source to bring large errors to the detection parameters.

[0003] The announcement number is: CN104677845B, which discloses an automatic detection device for optical properties of agricultural product tissues based on an integrating sphere, including a light source, a neutral density filter, an integrating sphere, an automatic sample stage, an automatic accessory stage, a motion control box, a spectrometer and a host computer PC. The invention uses a control system based on a single-chip microcomputer and a PLC to control the automatic movement of samples, and can switch freely between a single integrating sphere and a double integrating sphere, and can complete the measurement of optical property parameters at different positions of samples of different thicknesses. However, the light source used in the detection device is a laser light source, which can only measure optical property parameters at one wavelength at a time, and can only place one sample at a time, which is not suitable for measuring multiple samples.

[0004] Based on this, a device for automatically detecting optical characteristic parameters of biological tissue based on a single integrating sphere is now provided, which can eliminate the drawbacks of existing devices. Summary of the invention

[0005] The purpose of the present invention is to provide an automatic detection device for optical characteristic parameters of biological tissue based on a single-machine ball splitter, which can place multiple test samples at one time, and can automatically complete the collection of total reflectance and total transmittance spectra of multiple samples in turn, save data and calculate the absorption coefficient μ a and the reduced scattering system μ' s， Greatly improve the automation and efficiency of detection.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A device for automatically detecting optical characteristic parameters of biological tissues based on a single integrating sphere, comprising a light source module, a sample conveying module, an integrating sphere module, a front end cover shading module and a rear end cover shading module, wherein the integrating sphere module comprises an integrating sphere, wherein the integrating sphere is provided with an integrating sphere front end hole, an integrating sphere rear end hole and an integrating sphere upper hole, wherein the integrating sphere is fixedly arranged at one end of an integrating sphere support rod, wherein the integrating sphere support rod is fixedly arranged on an integrating sphere base, wherein the integrating sphere base is fixedly arranged on an upper end of an optical platform, wherein a light source module is arranged at a position close to one side of the integrating sphere front end hole, wherein a rear end cover shading module is arranged at a position close to one side of the integrating sphere rear end hole, wherein the sample conveying module and the front end cover shading module are symmetrically arranged on both sides of the integrating sphere, wherein the integrating sphere upper end hole A detection optical fiber is electrically connected to the hole, one end of the detection optical fiber is electrically connected to the spectrometer, the light source module, the sample delivery module, the integrating sphere module, the front cover shading module and the rear cover shading module are all connected to the motion control box control, the motion control box and the spectrometer are both connected to the host computer PC control, the light source module includes a light source transmitter, the output end of the light source transmitter is connected to a light source optical fiber, the other end of the light source optical fiber is connected to a focusing lens, the upper end of the optical platform is provided with a first adjustment mechanism for adjusting the working position of the focusing lens, the rear cover shading module includes a rear cover, the rear cover is fixedly arranged at one end of the rear cover support frame, and the upper end of the optical platform is provided with a second adjustment mechanism for adjusting the working position of the rear cover.

[0008] On the basis of the above technical solution, the present invention also provides the following optional technical solution:

[0009] In an optional scheme: the first adjustment mechanism includes a hollow guide rod, which is arranged on the light source optical fiber, and the hollow guide rod is fixedly arranged in the mounting holes of the output ends of the first support frame and the second support frame, the first support frame and the second support frame are respectively fixedly arranged on the upper ends of the first base and the second base, the first base and the second base are both fixedly arranged on the upper ends of the first threaded block, a first screw is cooperated in the threaded hole on one side of the first threaded block, the first screw is rotatably arranged inside the first linear guide rail, the first linear guide rail is fixedly arranged on the upper end of the optical platform, one end of the first threaded rod is fixedly connected to the output end of the first motor, the first motor is fixedly arranged on one side of the first linear guide rail, the light source emitter is a halogen tungsten lamp light source, and the focusing lens is coaxially arranged with the front end hole of the integrating sphere.

[0010] In an optional scheme: the second adjustment mechanism includes a rear end cover base, the rear end cover support frame is fixedly arranged on the upper end of the rear end cover base, the rear end cover base is fixedly arranged on the upper end of the second threaded block, a second threaded rod is cooperated in a threaded hole on one side of the second threaded block, the second threaded rod is rotatably arranged on the inner side of a sixth linear guide rail, the sixth linear guide rail is fixedly arranged on the upper end of the optical platform, one end of the second threaded rod is fixedly connected to the output end of the sixth motor, the sixth motor is fixedly arranged on one side of the sixth linear guide rail, and the rear end cover is coaxially arranged with the rear end hole of the integrating sphere.

[0011] In an optional scheme: the sample conveying module includes a rotating disk, a circular array of light-transmitting holes is provided on the rotating disk, sample clamping parts are provided at positions corresponding to the positions of the light-transmitting holes on one side of the rotating disk, the center of one side of the rotating disk is fixedly connected to the output end of the first servo, the first servo is fixedly arranged on one side of the first fixed bracket, the first fixed bracket is fixedly arranged on the upper end of the first servo base, the first servo base is fixedly arranged at one end of the rotating support frame, the rotating support frame is fixedly arranged on the output end of the second servo, the second servo is provided with a second fixed bracket, the second fixed bracket is fixedly arranged on one side of the second servo base, the second servo base is fixedly arranged on the upper end of the third threaded block, and a third threaded hole on one side of the third threaded block is provided with a third threaded hole The third threaded rod is rotatably arranged on the inner side of the second linear guide, one end of the third threaded rod is fixedly connected to the output end of the second motor, the second motor is fixedly arranged on one side of the second linear guide, a fourth threaded block and a first sliding block are fixedly arranged on the bottom end of the second linear guide, a fourth threaded rod is matched in a threaded hole on one side of the fourth threaded block, the fourth threaded rod is rotatably arranged on the inner side of the third linear guide, one end of the third linear guide is fixedly connected to the output end of the third motor, the third motor is fixedly arranged on one side of the third linear guide, the first sliding block is slidably arranged on the first lower slide rail, the first lower slide rail and the third linear guide are both fixedly arranged on the upper end of the optical platform, the third threaded rod is vertically arranged to the fourth threaded rod, and the fourth threaded rod is parallel to the first threaded rod.

[0012] In an optional scheme: the sample clamping part includes a fixed plate, a first connecting end is provided at the bottom end of the fixed plate, a second connecting end is provided on the rotating disk, the fixed plate is connected to the rotating disk through the first connecting end and the second connecting end, threaded holes are provided at both ends of the fixed plate, a movable plate is provided on the fixed plate, light-transmitting holes are provided on the fixed plate and the movable plate, and a spring is provided at the connection between the bottom end of the fixed plate and the rotating disk.

[0013] In an optional solution: the front end cover shading module includes a front end cover, the front end cover axis is horizontally parallel to the axes of the front end hole and the rear end hole of the integrating sphere, the front end cover is fixedly arranged at one end of the support rod, the support rod is fixedly arranged at one end of the front end cover support frame, the front end cover support frame is fixedly arranged at the upper end of the front end cover base, the front end cover base is fixedly arranged at the upper end of the fifth threaded block, a fifth threaded rod is matched in the threaded hole on one side of the fifth threaded block, the fifth threaded rod is rotatably arranged on the inner side of the fourth linear guide rail, one end of the fifth threaded rod is fixedly connected to the output end of the fourth motor, and the fourth motor is fixedly arranged at On one side of the fourth linear guide, a sixth threaded block and a second sliding block are fixedly provided at the bottom end of the fourth linear guide, a sixth threaded rod is cooperated in the threaded hole on one side of the sixth threaded block, the sixth threaded rod is rotatably arranged on the inner side of the fifth linear guide, one end of the sixth threaded rod is fixedly connected to the output end of the fifth motor, the fifth motor is fixedly arranged on one side of the fifth linear guide, the second sliding block is slidably arranged on the second lower slide rail, the second lower slide rail and the fifth linear guide are both fixedly arranged on the upper end of the optical platform, the fifth threaded rod and the sixth threaded rod are vertically arranged, and the sixth threaded rod is parallel to the first threaded rod.

[0014] In an optional scheme: the motion control box is provided with a first motor driver, a second motor driver, a third motor driver, a fourth motor driver, a fifth motor driver, a sixth motor driver, a first steering gear driver, and a second steering gear driver; the first motor driver, the second motor driver, the third motor driver, the fourth motor driver, the fifth motor driver, the sixth motor driver, the first steering gear driver, and the second steering gear driver are electrically controlled and connected to the first motor, the second motor, the third motor, the fourth motor, the fifth motor, the sixth motor, the first steering gear driver, and the second steering gear driver respectively; the first motor driver, the second motor driver, the third motor driver, the fourth motor driver, the fifth motor driver, the sixth motor driver, the first steering gear driver, and the second steering gear driver are all connected to the PLC and the single-chip microcomputer control; the PLC and the single-chip microcomputer are all connected to the host computer PC control.

[0015] In an optional solution: a light shield is provided on the upper end of the optical platform.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The present invention uses a rotating platform and an automatic sample clamping device to collect the sample reflectance, transmittance and collimated transmittance spectra and their reference spectra when only one integrating sphere is needed, thereby avoiding the problem of decreased detection accuracy caused by a change in the distance between the sample and the integrating sphere window hole due to the movement of the sample, improving the detection accuracy of the sample tissue optical parameters, and improving the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of the present invention.

[0019] Figure 2 It is a schematic diagram of the structure of the light source module of the present invention.

[0020] Figure 3 This is a schematic diagram of the structure of the sample delivery module of the present invention.

[0021] Figure 4 It is a schematic diagram of the structure of the sample clamping part of the present invention.

[0022] Figure 5 This is a schematic diagram of the structure of the integrating sphere module of the present invention.

[0023] Figure 6 It is a structural schematic diagram of the front end cover shading module of the present invention.

[0024] Figure 7 It is a structural schematic diagram of the rear end cover shading module of the present invention.

[0025] Figure 8 This is a schematic diagram of the present invention when detecting the transmitted dark spectrum.

[0026] Fig. 9 This is a schematic diagram of the present invention when detecting the transmission reference spectrum.

[0027] Fig.10 This is a schematic diagram of the present invention when detecting the transmission sample spectrum.

[0028] Fig.11 This is a schematic diagram of the present invention when detecting reflected dark spectrum.

[0029] Fig.12 This is a schematic diagram of the present invention when detecting the reflection reference spectrum.

[0030] Fig.13 This is a schematic diagram of the present invention when detecting the reflected sample spectrum.

[0031] Fig.14 This is a structural block diagram of the motion control box of the present invention.

[0032] Reference numerals: 1 light source module, 101 light source transmitter, 102 light source optical fiber, 103 hollow guide rod, 104 focusing lens, 105 first support frame, 106 second support frame, 107 first base, 108 second base, 109 first motor, 110 first linear guide rail;

[0033] 2 sample conveying module, 201 rotating disc, 202 sample clamping part, 202a spring, 202b fixed plate, 202c movable plate, 203 first steering gear, 204 first fixed bracket, 205 first steering gear base, 206 rotating support frame, 207 second steering gear, 208 second fixed bracket, 209 second steering gear base, 210 second motor, 211 second linear guide rail, 212 first lower slide rail, 213 third motor, 214 third linear guide rail;

[0034] 3 integrating sphere module, 301 integrating sphere, 301a integrating sphere front end hole, 301b integrating sphere rear end hole, 301c integrating sphere upper hole, 302 integrating sphere support rod, 303 integrating sphere base;

[0035] 4 front end cover shading module, 401 front end cover, 402 front end cover support frame, 403 front end cover base, 405 fourth motor, 406 fourth linear guide rail, 407 second lower slide rail, 408 fifth motor, 409 fifth linear guide rail;

[0036] 5 rear end cover shading module, 501 rear end cover, 502 rear end cover support frame, 503 rear end cover base, 504 sixth motor, 505 sixth linear guide rail;

[0037] 6 motion control box, 601 first motor driver, 602 second motor driver, 603 third motor driver, 604 fourth motor driver, 605 fifth motor driver, 606 sixth motor driver, 607 first servo driver, 608 second servo driver, 609 PLC, 610 single chip microcomputer;

[0038] 7. Spectrometer, 8. Host computer PC, 9. Detection optical fiber, 10. Optical platform. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments.

[0040] In one embodiment, Figure 1-Figure 14As shown, an automatic detection device for optical characteristic parameters of biological tissues based on a single integrating sphere comprises a light source module 1, a sample delivery module 2, an integrating sphere module 3, a front end cover shading module 4 and a rear end cover shading module 5, wherein the integrating sphere module 3 comprises an integrating sphere 301, wherein the integrating sphere 301 is provided with an integrating sphere front end hole 301a, an integrating sphere rear end hole 301b and an integrating sphere upper hole 301c, wherein the integrating sphere 301 is fixedly arranged at one end of an integrating sphere support rod 302, wherein the integrating sphere support rod 302 is fixedly arranged on an integrating sphere base 303, wherein the integrating sphere base 303 is fixedly arranged on the upper end of an optical platform 10, wherein the light source module 1 is arranged at a position close to one side of the integrating sphere front end hole 301a, wherein the rear end cover shading module 5 is arranged at a position close to one side of the integrating sphere rear end hole 301b, wherein the sample delivery module 2 and the front end cover shading module 4 are symmetrically arranged on both sides of the integrating sphere 301, wherein a detection optical fiber 9 is electrically connected to the integrating sphere upper hole 301c, wherein one end of the detection optical fiber 9 is electrically connected to a spectrometer 7, wherein the The light source module 1, the sample delivery module 2, the integrating sphere module 3, the front cover shading module 4 and the rear cover shading module 5 are all connected to the motion control box 6, and the motion control box 6 and the spectrometer 7 are all connected to the host computer PC8. The light source module 1 includes a light source transmitter 101, and the output end of the light source transmitter 101 is connected with a light source optical fiber 102, and the other end of the light source optical fiber 102 is connected with a focusing lens 104. The upper end of the optical platform 10 is provided with a first adjustment mechanism for adjusting the working position of the focusing lens 104, and the rear cover shading module 5 includes a rear cover 501, and the rear cover 501 is fixedly arranged at one end of the rear cover support frame 502. The upper end of the optical platform 10 is provided with a second adjustment mechanism for adjusting the working position of the rear cover 501. The first adjustment mechanism, the second adjustment mechanism and the third adjustment mechanism are used to adjust the working positions of the focusing lens 104, the rear cover 501 and the rotating disk 201 respectively, so as to facilitate the improvement of the detection accuracy of the optical parameters of the sample tissue and the improvement of the detection efficiency;

[0041] The first adjustment mechanism includes a hollow guide rod 103, which is arranged on the light source optical fiber 102, and the hollow guide rod 103 is fixed in the mounting holes of the output ends of the first support frame 105 and the second support frame 106. The first support frame 105 and the second support frame 106 are respectively fixed on the upper ends of the first base 107 and the second base 108. The first base 107 and the second base 108 are both fixed on the upper end of the first threaded block. A first screw is matched in a threaded hole on one side of the first threaded block. The first screw is rotatably arranged in the first linear guide rail 110. The first linear guide rail 110 is fixed on the upper end of the optical platform 10. The first screw One end of the threaded rod is fixedly connected to the output end of the first motor 109, the first motor 109 is fixedly arranged on one side of the first linear guide rail 110, the light source emitter 101 is a halogen tungsten lamp light source, the focusing lens 104 is coaxially arranged with the front end hole 301a of the integrating sphere, when in use, when working, start the first motor 109, the first motor 109 drives the first threaded rod to rotate, and drives the first threaded block to move under the action of the thread, and at the same time, the first threaded block drives the hollow guide rod 103 to move through the first support frame 105 and the second support frame 106, the hollow guide rod 103 drives the focusing lens 104 to move, and the light source emitter 101 sends a continuous spectrum, so that the light source can be moved.

[0042] The second adjustment mechanism includes a rear end cover base 503, the rear end cover support frame 502 is fixedly arranged on the upper end of the rear end cover base 503, the rear end cover base 503 is fixedly arranged on the upper end of the second threaded block, a second threaded rod is cooperated in a threaded hole on one side of the second threaded block, the second threaded rod is rotatably arranged on the inner side of the sixth linear guide 505, the sixth linear guide 505 is fixedly arranged on the upper end of the optical platform 10, one end of the second threaded rod is fixedly connected to the output end of the sixth motor 504, the sixth motor 504 is fixedly arranged on one side of the sixth linear guide 505, the rear end cover 501 is coaxially arranged with the rear end hole 301b of the integrating sphere, when in use, the sixth motor 504 drives the second threaded rod to rotate, and the second threaded rod drives the rear end cover 501 to move through the second threaded block and the rear end cover support frame 502, so that the rear end cover 501 is in close contact with one side of the rear end hole 301b of the integrating sphere, thereby facilitating the operation of the integrating sphere 301.

[0043] The sample delivery module 2 includes a rotating disk 201, a plurality of light-transmitting holes are arranged in a circular array on the rotating disk 201, a sample clamping portion 202 is arranged at a position corresponding to the plurality of light-transmitting holes on one side of the rotating disk 201, a center of one side of the rotating disk 201 is fixedly connected to an output end of a first steering gear 203, the first steering gear 203 is fixedly arranged on one side of a first fixed bracket 204, the first fixed bracket 204 is fixedly arranged on an upper end of a first steering gear base 205, and the first steering gear base 205 is fixedly arranged on a rotating support frame 206 The rotating support frame 206 is fixedly arranged on the output end of the second steering gear 207, and the second steering gear 207 is provided with a second fixed support 208, and the second fixed support 208 is fixedly arranged on one side of the second steering gear base 209, and the second steering gear base 209 is fixedly arranged on the upper end of the third threaded block, and a third threaded rod is matched in a threaded hole on one side of the third threaded block, and the third threaded rod is rotatably arranged on the inner side of the second linear guide rail 211, and one end of the third threaded rod is fixedly connected to the output end of the second motor 210, and the second motor 210 is fixed The first sliding block is slidably mounted on the first lower rail 212, and the first sliding block is slidably mounted on the first lower rail 212. The first lower rail 212 and the third linear guide 214 are connected to each other with a plurality of second linear guides. 4 are fixedly arranged on the upper end of the optical platform 10, the third threaded rod and the fourth threaded rod are arranged vertically, and the fourth threaded rod and the first threaded rod are arranged parallel to each other. When in use, a plurality of samples are clamped by a plurality of sample clamping parts 202, and then the second motor 210 and the third motor 213 are driven to adjust the working position of the rotating disk 201, and the first steering gear 203 can switch different samples on the rotating disk 201, and the second steering gear 207 can drive the rotating disk 201 to rotate, so as to realize the detection of reflectivity and transmittance spectra.

[0044] The sample clamping portion 202 includes a fixed plate 202b, a first connecting end is provided at the bottom end of the fixed plate 202b, a second connecting end is provided on the rotating disk 201, the fixed plate 202b is connected to the rotating disk 201 through the first connecting end and the second connecting end, threaded holes are provided at both ends of the fixed plate 202b, a movable plate 202c is provided on the fixed plate 202b, and light-transmitting holes are provided on the fixed plate 202b and the movable plate 202c. A spring 202a is provided at the connection between the bottom end of the fixed plate 202b and the rotating disk 201. When in use, elastic clamping of the sample can be achieved, and the sample clamping portion 202 is distributed on the rotating disk 201 in an array at 60° intervals.

[0045] The front end cover shading module 4 includes a front end cover 401, the axis of the front end cover 401 is horizontally parallel to the axis of the front end hole 301a and the rear end hole 301b of the integrating sphere, the front end cover 401 is fixedly arranged at one end of the support rod, the support rod is fixedly arranged at one end of the front end cover support frame 402, the front end cover support frame 402 is fixedly arranged at the upper end of the front end cover base 403, the front end cover base 403 is fixedly arranged at the upper end of the fifth threaded block, a fifth threaded rod is cooperated in the threaded hole on one side of the fifth threaded block, the fifth threaded rod is rotatably arranged on the inner side of the fourth linear guide 406, one end of the fifth threaded rod is fixedly connected to the output end of the fourth motor 405, the fourth motor 405 is fixedly arranged on one side of the fourth linear guide 406, a sixth threaded block and a second sliding block are fixedly arranged at the bottom end of the fourth linear guide 406, and a sixth threaded rod is cooperated in the threaded hole on one side of the sixth threaded block , the sixth threaded rod is rotatably arranged on the inner side of the fifth linear guide 409, one end of the sixth threaded rod is fixedly connected to the output end of the fifth motor 408, the fifth motor 408 is fixedly arranged on one side of the fifth linear guide 409, the second sliding block is slidably arranged on the second lower slide rail 407, the second lower slide rail 407 and the fifth linear guide 409 are both fixedly arranged on the upper end of the optical platform 10, the fifth threaded rod and the sixth threaded rod are vertically arranged, and the sixth threaded rod is parallel to the first threaded rod. When in use, driving the fourth motor 405 and the fifth motor 408 facilitates the adjustment of the working position of the front end cover 401, so that the front end cover 401 is in close contact with the front end hole 301a of the integrating sphere, so that the spectrometer 7 collects the spectrum in the integrating sphere 301 through the detection optical fiber 9 from the upper hole 301c of the integrating sphere, and the host computer PC8 receives the spectrum information collected by the spectrometer 7 to analyze the spectrum.

[0046] The motion control box 6 is provided with a first motor driver 601, a second motor driver 602, a third motor driver 603, a fourth motor driver 604, a fifth motor driver 605, a sixth motor driver 606, a first steering gear driver 607, and a second steering gear driver 608, wherein the first motor driver 601, the second motor driver 602, the third motor driver 603, the fourth motor driver 604, the fifth motor driver 605, the sixth motor driver 606, the first steering gear driver 607, and the second steering gear driver 608 are respectively connected to the first motor 109, the second motor 210, the third motor 213, the fourth motor 405, the fifth motor 408, the sixth motor 504, the first The servo 203 and the second servo 207 are electrically controlled and connected, and the first motor driver 601, the second motor driver 602, the third motor driver 603, the fourth motor driver 604, the fifth motor driver 605, the sixth motor driver 606, the first servo driver 607, and the second servo driver 608 are all controlled and connected with PLC609 and the single-chip microcomputer 610, and the PLC609 and the single-chip microcomputer 610 are all controlled and connected with the host computer PC8. When in use, it is convenient for the host computer PC8 to control the first motor 109, the second motor 210, the third motor 213, the fourth motor 405, the fifth motor 408, the sixth motor 504, the first servo 203, and the second servo 207 respectively.

[0047] The optical platform 10 is provided with a light shield at the upper end for protection during use.

[0048] The above embodiment discloses an automatic detection device for optical characteristic parameters of biological tissue based on a single integrating sphere, wherein when transmission dark spectrum acquisition is required, the thread block of the first linear guide rail 110 in the light source module 1 and the thread block of the second linear guide rail 211 in the sample delivery module 2 are respectively in Figure 1 The preset initial positions A0 and B0 are shown, the host computer PC 8 sends the preset position information to the single-chip computer 610 through the serial port, the single-chip computer 610 controls the PLC 609 to send a driving pulse, and drives the fourth motor 405, the fifth motor 408, and the sixth motor 504 through the fourth motor driver 604, the fifth motor driver 605, and the sixth motor driver 606, so as to drive the threaded block of the fourth linear guide 406 in the front cover shading module 4 and the threaded block of the sixth linear guide 505 in the rear cover shading module 5 to move to the position C1, that is, the front cover 401 and the front end hole 301a of the integrating sphere are coaxial and fully contacted with the position D1, that is, the rear cover 501 and the rear end hole 301b of the integrating sphere, the spectrometer 7 collects the spectrum in the integrating sphere 301 through the upper hole 301c of the integrating sphere through the detection optical fiber 9, and the host computer PC 8 receives the spectrum information collected by the spectrometer 7, analyzes and saves the transmitted dark spectrum;

[0049] When it is necessary to collect the transmission reference spectrum, the threaded block of the first linear guide 110 in the light source module 1, the threaded block of the second linear guide 211 in the sample delivery module 2, and the threaded block of the fourth linear guide 406 in the front cover shading module 4 are respectively in Figure 1 The preset initial positions A0, B0, and C0 are shown, and the host computer PC 8 sends the preset position information to the single-chip computer 610 through the serial port. The single-chip computer 610 controls the PLC 609 to send a driving pulse, and drives the sixth motor 504 through the sixth motor driver 606, so as to drive the threaded block of the sixth linear guide 505 in the rear end cover shading module 5 to move to the position D1, that is, the rear end cover 501 and the rear end hole 301b of the integrating sphere are fully in contact, and the light source enters the integrating sphere 301 through the front end hole 301a of the integrating sphere, and the host computer PC 8 saves the transmission reference spectrum;

[0050] When it is necessary to collect the sample transmission spectrum, the threaded block of the first linear guide rail 110 in the light source module 1 and the threaded block of the fourth linear guide rail 406 in the front end cover shading module 4 are respectively in Figure 1 The preset initial positions A0 and C0 are shown in the figure. The host computer PC 8 sends the preset position information to the single-chip microcomputer 610 through the serial port. The single-chip microcomputer 610 controls the PLC 609 to send a driving pulse, and drives the second motor 210, the third motor 213, and the sixth motor 504 through the second motor driver 602, the third motor driver 603, and the sixth motor driver 606, so as to drive the threaded block of the second linear guide 211 in the sample conveying module 2 and the threaded block of the sixth linear guide 505 in the rear end cover shading module 5 to move to the position B1, that is, the transmission hole of the sample holder 202 and the front end hole 301a of the integrating sphere are coaxial with the position D1, that is, the rear end cover 501 and the rear end hole 301b of the integrating sphere are fully contacted, and the light source passes through the first sample and enters the integrating sphere 301. The host computer PC 8 saves the transmission sample spectrum of the first sampling point. The host computer PC 8 sends information to the single-chip microcomputer 610 according to the designed sampling interval of 60°. The single-chip microcomputer 610 controls the PLC 609 sends a driving pulse, drives the first servo 203 through the first servo driver 607, and rotates the rotating disk 201 by 60°. The light source irradiates the second sample, and the host computer PC 8 saves the transmission sample spectrum of the second sampling point. The above steps are repeated until the transmission spectra of the six samples are all collected. After the transmission spectrum detection is completed, the host computer PC 8 sends information to the single-chip microcomputer 610 through the serial port. The single-chip microcomputer 610 controls the PLC 609 to send a driving pulse to all motor drivers, so that the thread blocks of all linear guides move to Figure 1 The preset initial position shown;

[0051] When it is necessary to collect the reflected dark spectrum, the threaded block of the second linear guide 211 in the sample delivery module 2, the threaded block of the fourth linear guide 406 in the front cover shading module 4, and the threaded block of the sixth linear guide 505 in the rear cover shading module 5 are respectively in Figure 1 The preset initial positions B0, C0, and D0 are shown, and the host computer PC 8 sends the preset position information to the single-chip microcomputer 610 through the serial port. The single-chip microcomputer 610 controls the PLC 609 to send a driving pulse, and drives the first motor 109 through the first motor driver 601, so as to drive the threaded block of the first linear guide rail 110 in the light source module 1 to move to the position A1, that is, the hollow guide rod 103 in the light source module 1 extends into the integrating sphere 301 through the front end hole 301a of the integrating sphere, and the light source emitter 101 is turned on to emit a spectrum, and the host computer PC 8 saves the reflected dark spectrum;

[0052] When it is necessary to collect the reflection reference spectrum, the threaded block of the second linear guide rail 211 in the sample delivery module 2 and the threaded block of the fourth linear guide rail 406 in the front cover shading module 4 are respectively in Figure 1 The preset initial positions B0 and C0 are shown, the host computer PC 8 sends the preset position information to the single-chip microcomputer 610 through the serial port, and the single-chip microcomputer 610 controls the PLC 609 to send a driving pulse, and drives the first motor 109 and the sixth motor 504 through the first motor driver 601 and the sixth motor driver 606, so as to drive the threaded block of the first linear guide 110 in the light source module 1 and the threaded block of the sixth linear guide 505 in the rear end cover shading module 5 to move to the position A1, that is, the hollow guide rod 103 in the light source module 1 extends from the front end hole 301a of the integrating sphere into the integrating sphere 301 and fully contacts the position D1, that is, the rear end cover 501 and the rear end hole 301b of the integrating sphere, and the host computer PC 8 saves the reflection reference spectrum;

[0053] When it is necessary to collect the reflected sample spectrum, the threaded block of the second linear guide 211 in the sample delivery module 2, the threaded block of the fourth linear guide 406 in the front cover shading module 4, and the threaded block of the sixth linear guide 505 in the rear cover shading module 5 are respectively in Figure 1The preset initial positions B0, C0, and D0 are shown in the figure. The host computer PC 8 sends the preset information to the single-chip microcomputer 610 through the serial port. The single-chip microcomputer 610 controls the PLC 609 to send a driving pulse, and drives the first motor 109 through the first motor driver 601, so as to drive the threaded block of the first linear guide 110 in the light source module 1 to move to the position A1, that is, the hollow guide rod 103 in the light source module 1 extends into the integrating sphere 301 through the front end hole 301a of the integrating sphere, and drives the second servo 207 through the second servo driver 608, so that the rotating disk 201 in the sample conveying module 2 rotates vertically by 180°. The host computer PC 8 sends the preset position information to the single-chip microcomputer 610 through the serial port. The single-chip microcomputer 610 controls the PLC 609 to send a driving pulse, and drives the first motor 109 through the first motor driver 601, so as to drive the threaded block of the first linear guide 110 in the light source module 1 to move to the position A1, that is, the hollow guide rod 103 in the light source module 1 extends into the integrating sphere 301 through the front end hole 301a of the integrating sphere, and drives the second servo 207 through the second servo driver 608, so that the rotating disk 201 in the sample conveying module 2 rotates vertically by 180°. 609 sends a driving pulse, drives the second motor 210 and the third motor 213 through the second motor driver 602 and the third motor driver 603, drives the thread block of the second linear guide 211 in the sample conveying module 2 to move to position B2, that is, the transmission hole of the sample holder 202 is coaxial with the rear end hole 301b of the integrating sphere, and the host computer PC 8 saves the reflection sample spectrum of the first sampling point. The host computer PC 8 sends information to the single-chip microcomputer 610 according to the designed sampling interval of 60°, and the single-chip microcomputer 610 controls the PLC 609 to send a driving pulse, drives the first steering gear 203 through the first steering gear driver 607, drives the rotating disk 201 to rotate 60°, so that the light source irradiates the second sample, and the host computer PC 8 saves the reflection sample spectrum of the second sampling point, repeats the above steps until the reflection spectra of the six samples are collected, and after the reflection spectrum detection is completed, the host computer PC8 sends information to the single-chip microcomputer 610 through the serial port, and the single-chip microcomputer 610 controls the PLC 609 to send driving pulses to all drivers, driving all the thread blocks to move to Figure 1 Initial position of the preset shown.

[0054] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art who is familiar with the present technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. A device for automatically detecting optical characteristic parameters of biological tissues based on a single integrating sphere, comprising a light source module (1), a sample delivery module (2), an integrating sphere module (3), a front cover shading module (4) and a rear cover shading module (5), characterized in that: The integrating sphere module (3) comprises an integrating sphere (301), wherein the integrating sphere (301) is provided with an integrating sphere front end hole (301a), an integrating sphere rear end hole (301b) and an integrating sphere upper hole (301c); the integrating sphere (301) is fixedly arranged at one end of an integrating sphere support rod (302); the integrating sphere support rod (302) is fixedly arranged on an integrating sphere base (303); the integrating sphere base (303) is fixedly arranged on the upper end of an optical platform (10); a light source module (1) is arranged at a position close to one side of the integrating sphere front end hole (301a); a rear end cover shading module (5) is arranged at a position close to one side of the integrating sphere rear end hole (301b); the sample delivery module (2) and the front end cover shading module (4) are symmetrically arranged on both sides of the integrating sphere (301); a detection optical fiber (9) is electrically connected to the integrating sphere upper hole (301c); one end of the detection optical fiber (9) is connected to a spectrometer (7); The optical platform (10) is electrically connected, the light source module (1), the sample delivery module (2), the integrating sphere module (3), the front cover shading module (4) and the rear cover shading module (5) are all connected to the motion control box (6), the motion control box (6) and the spectrometer (7) are all connected to the host computer PC (8), the light source module (1) comprises a light source transmitter (101), the output end of the light source transmitter (101) is connected to a light source optical fiber (102), the other end of the light source optical fiber (102) is connected to a focusing lens (104), the upper end of the optical platform (10) is provided with a first adjustment mechanism for adjusting the working position of the focusing lens (104), the rear cover shading module (5) comprises a rear cover (501), the rear cover (501) is fixedly arranged at one end of the rear cover support frame (502), and the upper end of the optical platform (10) is provided with a second adjustment mechanism for adjusting the working position of the rear cover (501).

2. The automatic detection device for optical characteristic parameters of biological tissue based on a single integrating sphere according to claim 1, characterized in that: The first adjustment mechanism comprises a hollow guide rod (103), the hollow guide rod (103) being arranged on the light source optical fiber (102), the hollow guide rod (103) being fixedly arranged in the mounting holes at the output ends of the first support frame (105) and the second support frame (106), the first support frame (105) and the second support frame (106) being fixedly arranged at the upper ends of the first base (107) and the second base (108), respectively, the first base (107) and the second base (108) being fixedly arranged at the upper ends of the first threaded block, A first screw rod is provided in a threaded hole on one side of the first threaded block. The first screw rod is rotatably arranged inside a first linear guide rail (110). The first linear guide rail (110) is fixedly arranged on the upper end of the optical platform (10). One end of the first screw rod is fixedly connected to an output end of a first motor (109). The first motor (109) is fixedly arranged on one side of the first linear guide rail (110). The light source emitter (101) is a tungsten halogen light source. The focusing lens (104) is coaxially arranged with a front end hole (301a) of an integrating sphere.

3. The automatic detection device for optical characteristic parameters of biological tissue based on a single integrating sphere according to claim 2, characterized in that: The second adjustment mechanism comprises a rear end cover base (503), the rear end cover support frame (502) is fixedly arranged on the upper end of the rear end cover base (503), the rear end cover base (503) is fixedly arranged on the upper end of the second threaded block, a second threaded rod is matched in a threaded hole on one side of the second threaded block, the second threaded rod is rotatably arranged on the inner side of a sixth linear guide rail (505), the sixth linear guide rail (505) is fixedly arranged on the upper end of the optical platform (10), one end of the second threaded rod is fixedly connected to the output end of a sixth motor (504), the sixth motor (504) is fixedly arranged on one side of the sixth linear guide rail (505), and the rear end cover (501) is coaxially arranged with the rear end hole (301b) of the integrating sphere.

4. The automatic detection device for optical characteristic parameters of biological tissue based on a single integrating sphere according to claim 3, characterized in that: The sample conveying module (2) comprises a rotating disk (201), a plurality of light-transmitting holes are arranged in a circular array on the rotating disk (201), a sample clamping portion (202) is arranged at positions corresponding to the positions of the plurality of light-transmitting holes on one side of the rotating disk (201), a center of one side of the rotating disk (201) is fixedly connected to an output end of a first steering gear (203), the first steering gear (203) is fixedly arranged on one side of a first fixed bracket (204), the first fixed bracket (204) is fixedly arranged on the upper end of a first steering gear base (205), the first steering gear base (205) is fixedly arranged on one end of a rotating support frame (206), the rotating support frame (206) is fixedly arranged on the output end of a second steering gear (207), the second steering gear (207) is provided with a second fixed bracket (208), the second fixed bracket (208) is fixedly arranged on one side of a second steering gear base (209), the second steering gear base (209) is fixedly arranged on the upper end of a third threaded block, and a threaded portion on one side of the third threaded block A third threaded rod is provided in the groove hole, the third threaded rod is rotatably arranged inside the second linear guide rail (211), one end of the third threaded rod is fixedly connected to the output end of the second motor (210), the second motor (210) is fixedly arranged on one side of the second linear guide rail (211), a fourth threaded block and a first sliding block are fixedly provided at the bottom end of the second linear guide rail (211), a fourth threaded rod is provided in the threaded hole on one side of the fourth threaded block, the fourth threaded rod is rotatably arranged inside the third linear guide rail (214), one end of the third linear guide rail (214) is fixedly connected to the output end of the third motor (213), the third motor (213) is fixedly arranged on one side of the third linear guide rail (214), the first sliding block is slidably arranged on the first lower slide rail (212), the first lower slide rail (212) and the third linear guide rail (214) are both fixedly arranged on the upper end of the optical platform (10), the third threaded rod is arranged perpendicularly to the fourth threaded rod, and the fourth threaded rod is arranged parallel to the first threaded rod.

5. The automatic detection device for optical characteristic parameters of biological tissue based on a single integrating sphere according to claim 4, characterized in that: The sample clamping portion (202) comprises a fixed plate (202b), a first connecting end is provided at the bottom end of the fixed plate (202b), a second connecting end is provided on the rotating disk (201), the fixed plate (202b) is connected to the rotating disk (201) via the first connecting end and the second connecting end, threaded holes are provided at both ends of the fixed plate (202b), a movable plate (202c) is provided on the fixed plate (202b), light-transmitting holes are provided on the fixed plate (202b) and the movable plate (202c), and a spring (202a) is provided at the connection between the bottom end of the fixed plate (202b) and the rotating disk (201).

6. The automatic detection device for optical characteristic parameters of biological tissue based on a single integrating sphere according to claim 5, characterized in that: The front end cover shading module (4) comprises a front end cover (401), the axis of the front end cover (401) is horizontally parallel to the axis of the front end hole (301a) and the rear end hole (301b) of the integrating sphere, the front end cover (401) is fixedly arranged at one end of a support rod, the support rod is fixedly arranged at one end of a front end cover support frame (402), the front end cover support frame (402) is fixedly arranged at the upper end of a front end cover base (403), the front end cover base (403) is fixedly arranged at the upper end of a fifth threaded block, a fifth threaded rod is matched in a threaded hole on one side of the fifth threaded block, the fifth threaded rod is rotatably arranged on the inner side of a fourth linear guide rail (406), one end of the fifth threaded rod is fixedly connected to the output end of a fourth motor (405), and the fourth motor (405) is fixedly arranged at the upper end of a fifth threaded block, and the fifth threaded rod is fixedly arranged in a threaded hole on one side of the fifth threaded block, and the fifth threaded rod is rotatably arranged on the inner side of a fourth linear guide rail (406), one end of the fifth threaded rod is fixedly connected to the output end of a fourth motor (405), and the fourth motor (405) is fixedly arranged at the upper end of a fifth threaded block. The fourth linear guide (406) is fixedly provided at one side of the fourth linear guide (406), a sixth threaded block and a second sliding block are fixedly provided at the bottom end of the fourth linear guide (406), a sixth threaded rod is matched in a threaded hole on one side of the sixth threaded block, the sixth threaded rod is rotatably provided inside the fifth linear guide (409), one end of the sixth threaded rod is fixedly connected to the output end of the fifth motor (408), the fifth motor (408) is fixedly provided at one side of the fifth linear guide (409), the second sliding block is slidably provided on the second lower slide rail (407), the second lower slide rail (407) and the fifth linear guide (409) are both fixedly provided at the upper end of the optical platform (10), the fifth threaded rod is vertically provided to the sixth threaded rod, and the sixth threaded rod is parallelly provided to the first threaded rod.

7. The automatic detection device for optical characteristic parameters of biological tissue based on a single integrating sphere according to claim 1, characterized in that: A light shield is provided at the upper end of the optical platform (10).

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