Sample analyzer and electrolyte determination calibration method thereof

Abstract

The invention is applicable to the field of in vitro diagnostic equipment, and discloses a sample analyzer and a calibration method for electrolyte determination in the sample analyzer, the sample analyzer comprises a detection container, a first sample distribution assembly, an electrode assembly, a liquid discharge assembly and a controller, the controller is configured to execute the following calibration actions: controlling the liquid discharge assembly to drive at least three calibration liquids with different target ion concentrations to respectively flow through the electrode assembly and measure corresponding first measurement parameters, and performing calibration according to at least three first measurement parameters measured by the electrode assembly on the at least three calibration liquids, obtaining a calibration result for representing the relationship between the target ion concentration and the measured parameters; the controller is further configured to execute the following electrolyte determination actions: obtaining a target ion determination result of the sample according to the calibration result and a second determination parameter measured by the electrode assembly to the liquid to be measured. By adopting the calibration scheme for electrolyte determination, the accuracy of the electrolyte determination result of the sample analyzer on the sample can be improved.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnostic equipment, and in particular to a sample analyzer and a calibration method for electrolyte determination in the sample analyzer. Background Art

[0002] A sample analyzer provided in the related art is provided with an electrolyte measurement module, and the electrolyte measurement module includes a detection container and an electrode assembly, and the electrode assembly is used to perform electrolyte measurement on the test liquid flowing out of the detection container. Before the electrolyte measurement, the electrolyte measurement module is generally calibrated. In the related art, the calibration method of the electrolyte measurement module is: a calibration solution made of two standard solutions with known target ion concentrations is passed through the electrode assembly, and the measurement parameters of the electrode assembly are measured when the two calibration solutions flow through the electrode assembly. According to the measurement parameters measured by the two standard solutions, a calibration result for characterizing the linear relationship between the target ion concentration and the measurement parameter is obtained. When performing electrolyte measurement on the sample, the target ion measurement result of the sample can be obtained according to the measurement parameters measured by the electrode assembly and the calibration result.

[0003] For some samples, the target ion determination results calculated using the calibration scheme of the above-mentioned related technology will be more accurate. However, for those samples whose target ion concentrations are lower or higher than the concentration range of the two standard solutions, the target ion determination results calculated based on the calibration results will have a large deviation from the actual target ion concentration of the sample, that is, the actual target ion concentration of the sample and the determination parameters no longer satisfy the linear relationship between the target ion concentration and the determination parameters in the calibration results, which seriously affects the accuracy of the target ion determination results of the sample, especially in urine samples. Because the concentration distribution range of the target ion in urine samples is wider than that of serum, the deviation of the target ion determination results will also be greater. Summary of the invention

[0004] The first object of the present invention is to provide a sample analyzer, which aims to solve the technical problem that the calibration results obtained by using two standard solutions to perform electrolyte measurement calibration in the related art have large deviations in the target ion measurement results of some samples.

[0005] To achieve the above object, the present invention provides a solution: a sample analyzer, comprising:

[0006] A detection container, wherein the detection container has an inner cavity and a liquid discharge port communicated with the inner cavity;

[0007] a first sample dispensing component, the first sample dispensing component being used to dispense a sample into the inner cavity;

[0008] An electrode assembly, the electrode assembly being used to measure target ions of a test liquid discharged from the liquid discharge port and containing at least a sample;

[0009] A liquid discharge assembly, the liquid discharge assembly is used to drive the test liquid containing at least the sample to flow through the electrode assembly and to drive a calibration liquid containing at least a standard solution to flow through the electrode assembly;

[0010] A controller, wherein the controller is configured to perform the following calibration actions: controlling the liquid discharge component to drive at least three calibration liquids with different target ion concentrations to flow through the electrode component respectively and measure corresponding first measurement parameters, and obtaining a calibration result for characterizing the relationship between the target ion concentration and the measurement parameter according to at least three first measurement parameters measured by the electrode component for the at least three calibration liquids, wherein the calibration result includes at least one of a calibration parameter, a calibration curve, and a calibration relationship;

[0011] The controller is also configured to perform the following electrolyte measurement action: control the drainage component to drive the test liquid to flow from the detection container through the electrode component and obtain a corresponding second measurement parameter, and obtain the target ion measurement result of the sample based on the calibration result and the second measurement parameter measured by the electrode assembly for the test liquid.

[0012] As an embodiment, the sample analyzer further comprises an internal standard solution dispensing component, and the internal standard solution dispensing component is used to dispense the internal standard solution into the detection container;

[0013] The liquid discharge assembly is also used to drive a reference solution containing at least the internal standard solution to flow through the electrode assembly;

[0014] The controller controls the liquid discharge component to drive the calibration liquid to flow through the electrode component and measure the corresponding first measurement parameter, including: controlling the liquid discharge component to drive the calibration liquid and the reference liquid to flow through the electrode component respectively and measure the corresponding first measurement parameter;

[0015] The controller controls the liquid discharge component to drive the liquid to be tested to flow through the electrode component and obtain the corresponding second measurement parameter, including: controlling the liquid discharge component to drive the liquid to be tested and the reference liquid to flow through the electrode component respectively and measuring the corresponding second measurement parameter.

[0016] As an embodiment, the first measurement parameter is a potential difference between the calibration solution flowing through the electrode assembly and the reference solution flowing through the electrode assembly;

[0017] The second measurement parameter is the potential difference between the test solution flowing through the electrode assembly and the reference solution flowing through the electrode assembly.

[0018] As an embodiment, the at least three calibration solutions include a first calibration solution including at least a first standard solution, a second calibration solution including at least a second standard solution, and a third calibration solution including at least a third standard solution, the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution, the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution and greater than the target ion concentration of the third standard solution.

[0019] As an embodiment, the third standard solution and the calibration solution at least comprising the third standard solution are both diluents; and / or,

[0020] The target ion concentration difference between the first standard solution and the second standard solution is greater than the target ion concentration difference between the second standard solution and the third standard solution.

[0021] As an embodiment, the control of the liquid discharge component drives at least three calibration liquids with different target ion concentrations to flow through the electrode component respectively and measure corresponding first measurement parameters, and obtaining a calibration result for characterizing the relationship between the target ion concentration and the measurement parameter according to at least three first measurement parameters measured by the electrode component for the at least three calibration liquids, including:

[0022] The drainage component is controlled to drive the first calibration solution and the reference solution, which at least include the first standard solution, to flow through the electrode component respectively and measure a first potential difference; the drainage component is controlled to drive the second calibration solution and the reference solution, which at least include the second standard solution, to flow through the electrode component respectively and measure a second potential difference; the drainage component is controlled to drive the third calibration solution and the reference solution, which at least include the third standard solution, to flow through the electrode component respectively and measure a third potential difference; and the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter is obtained at least according to the first potential difference, the second potential difference and the third potential difference.

[0023] As an embodiment, the at least three calibration solutions include a first calibration solution including at least a first standard solution, a second calibration solution including at least a second standard solution, a third calibration solution including at least a third standard solution, and a fourth calibration solution including at least a fourth standard solution, the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution and less than the target ion concentration of the fourth standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution and greater than the target ion concentration of the third standard solution; or,

[0024] The at least three calibration solutions include a first calibration solution including at least a first standard solution, a second calibration solution including at least a second standard solution, and a fourth calibration solution including at least a fourth standard solution, the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution and less than the target ion concentration of the fourth standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution.

[0025] As an embodiment, the third standard solution and the third calibration solution prepared by including the third standard solution are both diluents; and / or,

[0026] The target ion concentration difference between the first standard solution and the second standard solution is greater than the target ion concentration difference between the second standard solution and the third standard solution, and the target ion concentration difference between the first standard solution and the second standard solution is greater than the target ion concentration difference between the fourth standard solution and the first standard solution.

[0027] As an embodiment, the sample analyzer also includes a diluent dispensing component, which is used to dispense the diluent into the detection container, the calibration liquid is made of the standard solution and the diluent, the test liquid is made of the sample and the diluent, and the reference liquid is made of the internal standard solution and the diluent.

[0028] As an embodiment, the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter is obtained based on at least three of the first measurement parameters measured by the electrode assembly on the at least three calibration liquids, including: at least one of the following calibration results is obtained based on two of the first measurement parameters measured by the electrode assembly on any two of the calibration liquids having adjacent target ion concentrations: a set of calibration parameters, a calibration straight line, and a calibration relationship.

[0029] As an embodiment, the electrode assembly is used to measure at least two target ions in the test liquid or the calibration liquid, and the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter is obtained according to the at least three first measurement parameters measured by the electrode assembly for the at least three calibration liquids, including: according to the at least three first measurement parameters measured by the electrode assembly for the at least three calibration liquids, corresponding to each target ion, at least two groups of calibration parameters of a linear function for characterizing the relationship between the target ion concentration and the measurement parameter are obtained, each group of the calibration parameters includes a calibration slope and a calibration intercept of a linear function for characterizing the relationship between the target ion concentration and the measurement parameter, the calibration slope and the calibration intercept in each group of the calibration parameters are obtained from the two first measurement parameters measured by two calibration liquids with adjacent target ion concentrations, and the two calibration slopes of each group of the calibration parameters are different; and / or,

[0030] The electrode assembly is used to measure at least two target ions in the test liquid or the calibration liquid, and the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter is obtained based on the at least three first measurement parameters measured by the electrode assembly for the at least three calibration liquids, including: based on the at least three first measurement parameters measured by the electrode assembly for the at least three calibration liquids, corresponding to each target ion, at least two calibration lines for characterizing the relationship between the target ion concentration and the measurement parameter are obtained, the at least two calibration lines obtained for each target ion are connected in sequence and have different slopes, and each calibration line is obtained from two first measurement parameters measured by two calibration liquids with adjacent target ion concentrations.

[0031] As an embodiment, the sample analyzer pre-stores at least one of the following preset conditions: reaching a first preset time after the sample analyzer is turned on; reaching a second preset time since the calibration working mode was last executed; the time for the sample analyzer to continuously execute the electrolyte determination working mode reaches a third preset time; the cumulative time for the sample analyzer to execute the electrolyte determination working mode reaches a fourth preset time since the calibration working mode was last executed; the number of times the sample analyzer continuously executes the electrolyte determination working mode reaches a first preset number; the cumulative number of times the sample analyzer executes the electrolyte determination working mode reaches a second preset number since the calibration working mode was last executed; reaching a preset time point of a preset period, the preset period is every day, every two days, every three days, every four days, every five days, or every week;

[0032] The controller is further configured to: execute the calibration action when any one of the preset conditions is met.

[0033] As an embodiment, the sample analyzer further comprises a standard solution dispensing component, the standard solution dispensing component is used to dispense the standard solution into the detection container, the standard solution dispensing component and the first sample dispensing component are the same component or two independent components; and / or,

[0034] The electrode assembly includes at least one ion selective electrode and a reference electrode. The drainage assembly drives the liquid to be tested in the detection container to flow through the electrode assembly, including driving the liquid to be tested in the detection container to flow through the ion selective electrode and the reference electrode in sequence. The drainage assembly drives the calibration liquid to flow through the electrode assembly, including driving the calibration liquid in the detection container to flow through the ion selective electrode and the reference electrode in sequence. The at least one ion selective electrode includes at least one of a sodium ion selective electrode, a chloride ion selective electrode, and a potassium ion selective electrode.

[0035] As an embodiment, the sample analyzer further comprises a reagent dispensing component, a second sample dispensing component and at least one optical measurement component, wherein the second sample dispensing component is used to draw a sample from a sample container and distribute it to a reaction container, the reagent dispensing component is used to draw a reagent from a reagent container and distribute it to the reaction container, and the optical measurement component is used to perform optical measurement on a reaction solution made of at least the sample dispensed by the second sample dispensing component and the reagent dispensed by the reagent dispensing component;

[0036] The at least one optical assay component includes at least one of a biochemical assay component, an immunoassay component, and a coagulation assay component;

[0037] The second sample distribution component and the first sample distribution component are the same component or two different components.

[0038] A second object of the present invention is to provide a calibration method for electrolyte determination in a sample analyzer, the calibration method comprising the following steps:

[0039] Controlling the liquid discharge component to drive at least three calibration solutions with different target ion concentrations to flow through the electrode component respectively and measure corresponding first measurement parameters;

[0040] According to the at least three first measurement parameters measured by the at least three calibration solutions, a calibration result for characterizing the relationship between the target ion concentration and the measurement parameters is obtained, and the calibration result includes at least one of a calibration parameter, a calibration curve, and a calibration relationship.

[0041] As an embodiment, the control liquid discharge component drives at least three calibration solutions with different target ion concentrations to flow through the electrode components respectively and measure the corresponding first measurement parameters, including:

[0042] The drainage component is controlled to drive the first calibration solution containing at least the first standard solution and the reference solution containing at least the internal standard solution to flow through the electrode component respectively and measure the first potential difference, the drainage component is controlled to drive the second calibration solution containing at least the second standard solution and the reference solution containing at least the internal standard solution to flow through the electrode component respectively and measure the second potential difference, and the drainage component is controlled to drive the third calibration solution containing at least the third standard solution and the reference solution containing at least the internal standard solution to flow through the electrode component respectively and measure the third potential difference.

[0043] As an embodiment, the third standard solution and the calibration solution at least comprising the third standard solution are both diluents; and / or,

[0044] The target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution and greater than the target ion concentration of the third standard solution.

[0045] The sample analyzer and the calibration method for electrolyte determination in the sample analyzer provided by the present invention use at least three calibration solutions with different target ion concentrations to calibrate the electrolyte determination, which is equivalent to adding at least one calibration solution to calibrate the electrolyte determination on the basis of the calibration scheme of the related art. This is conducive to obtaining calibration results with a wider target ion concentration range, so that the calibration results can be used to accurately determine the target ion concentrations of more samples, thereby ensuring the accuracy of the electrolyte determination results of different samples by the sample analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0047] Figure 1 It is a schematic diagram of the liquid path of the electrolyte determination part of the sample analyzer provided in an embodiment of the present invention.

[0048] Explanation of the accompanying drawings: 10, sample analyzer; 100, electrode assembly; 110, ion selective electrode; 120, reference electrode; 200, detection container; 300, drainage assembly; 310, drainage power component; 320, waste liquid channel; 400, first sample distribution assembly; 410, first sample needle; 500, diluent distribution assembly; 600, internal standard liquid distribution assembly. DETAILED DESCRIPTION

[0049] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0050] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0051] It should also be noted that when an element is referred to as being "fixed on" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element at the same time. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or may be indirectly connected to the other element through an intermediate element.

[0052] In addition, the descriptions of "first", "second", etc. in the present invention are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0053] Embodiment 1:

[0054] like Figure 1 As shown, the sample analyzer 10 provided in the first embodiment of the present invention includes an electrode assembly 100 and a detection container 200. The electrode assembly 100 is used to measure target ions (i.e., electrolyte measurement, which mainly measures the concentration of target ions in the test liquid) in the test liquid flowing out of the detection container 200. The test liquid at least contains a sample.

[0055] As an embodiment, the detection container 200 has an inner cavity and a liquid discharge port connected to the inner cavity, and the liquid discharge port is used to allow the liquid to be tested to flow from the inner cavity to the electrode assembly 100. The inner cavity is used to provide a place for accommodating the sample and the liquid to be tested. The electrode assembly 100 is used to measure the target ions of the liquid to be tested that is discharged from the liquid discharge port and at least contains the sample.

[0056] As an embodiment, the sample analyzer 10 further includes a liquid discharge assembly 300, which is used to drive the liquid to flow through the electrode assembly 100, that is, the liquid discharge assembly 300 is used to provide a driving force for the liquid to flow through the electrode assembly 100. In this embodiment, the liquid discharge assembly 300 is used to drive the test liquid containing at least the sample to flow through the electrode assembly 100 and to drive the calibration liquid containing at least the standard solution to flow through the electrode assembly 100.

[0057] As an embodiment, the sample analyzer 10 further includes a first sample dispensing component 400, which is used to distribute the sample into the inner cavity of the detection container 200. The first sample dispensing component 400 is mainly used to achieve quantitative distribution of the sample. The first sample dispensing component 400 injects the sample into the detection container 200 by dispensing.

[0058] As an embodiment, the sample analyzer 10 further includes a controller, which is electrically connected to at least one of the electrode assembly 100 , the drainage assembly 300 , and the first sample dispensing assembly 400 .

[0059] As an embodiment, the controller is electrically connected to the electrode assembly 100, the liquid discharge assembly 300, and the first sample dispensing assembly 400. The controller is used to control the operation of the liquid discharge assembly 300 and the first sample dispensing assembly 400, and is used to obtain the target ion determination result of the sample according to the electrical parameters of the electrode assembly 100 when the test liquid flows through the electrode assembly 100.

[0060] As an embodiment, the controller is configured to perform the following calibration action: control the drainage component 300 to drive at least three calibration liquids with different target ion concentrations to flow through the electrode component 100 respectively and measure the corresponding first measurement parameters, and obtain a calibration result for characterizing the relationship between the target ion concentration and the measurement parameter according to the at least three first measurement parameters measured by the electrode component 100 for the at least three calibration liquids, and the calibration result includes at least one of the calibration parameter, the calibration curve, and the calibration relationship. The controller is also configured to perform the following electrolyte measurement action: control the drainage component 300 to drive the test liquid to flow from the detection container 200 through the electrode component 100 and obtain the corresponding second measurement parameter, and obtain the target ion measurement result of the sample according to the calibration result and the second measurement parameter measured by the electrode component 100 for the test liquid. The calibration operation of electrolyte measurement, also known as the calibration operation of electrolyte measurement, is mainly used to determine the coefficient in the relationship between the target ion concentration and the measurement parameter. In this embodiment, at least three calibration solutions with different target ion concentrations are used to calibrate the electrolyte measurement, which is beneficial to obtaining calibration results with a wider target ion concentration range, so that the calibration results can be used to accurately measure the target ion concentrations of more samples, thereby ensuring the accuracy of the electrolyte measurement results of different samples by the sample analyzer 10.

[0061] As an embodiment, the electrode assembly 100 includes a reference electrode 120 and at least one ion selective electrode 110, the discharge assembly 300 drives the test liquid in the detection container 200 to flow through the electrode assembly 100, including driving the test liquid in the detection container 200 to flow through the ion selective electrode 110 and the reference electrode 120 in sequence, the discharge assembly 300 drives the calibration liquid to flow through the electrode assembly 100, including driving the calibration liquid in the detection container 200 to flow through the ion selective electrode 110 and the reference electrode 120 in sequence, and the at least one ion selective electrode 110 includes at least one of a sodium ion selective electrode 110, a chloride ion selective electrode 110, and a potassium ion selective electrode 110. In this embodiment, the ion selective electrode 110 and the reference electrode 120 are arranged side by side, and the measurement parameters are obtained by the test liquid flowing through the ion selective electrode 110 and the reference electrode 120 in sequence. Of course, in specific applications, the arrangement of the electrode assembly 100 is not limited to this.

[0062] As an embodiment, the electrode assembly 100 includes a reference electrode 120, a sodium ion selective electrode 110, a chloride ion selective electrode 110, and a potassium ion selective electrode 110. In this embodiment, the sodium ion concentration, the chloride ion concentration, and the potassium ion concentration of the sample can be measured.

[0063] As an embodiment, the electrode assembly 100 further includes a measurement channel, and the liquid discharge port is connected to the liquid discharge assembly 300 through the measurement channel. The reference electrode 120 and the ion selective electrode 110 are at least partially disposed in the measurement channel, so that the liquid flowing through the measurement channel can contact the reference electrode 120 and the ion selective electrode 110.

[0064] As an embodiment, the liquid discharge assembly 300 includes a liquid discharge power component 310 and a waste liquid channel 320. The liquid discharge power component 310 is used to drive the liquid in the inner cavity of the detection container 200 to flow through the liquid discharge port and the electrode assembly 100 in sequence and then be discharged to the waste liquid channel 320. The liquid discharge power component 310 is mainly used to provide a driving force for the liquid in the detection container 200 to flow out of the liquid discharge port and flow through the electrode assembly 100. The waste liquid channel 320 is connected to the waste liquid collection container to discharge the liquid discharged by the liquid discharge power component 310 to the waste liquid collection container.

[0065] As an embodiment, the sample is serum or plasma or urine or other body fluids. After the sample is collected from the patient, it is stored in a sample container, and the first sample distribution component 400 is used to absorb the sample from the sample container and distribute at least part of the absorbed sample into the detection container 200. The sample container is provided with an identification code, which includes at least one of a bar code, a QR code, and a radio frequency code.

[0066] As an embodiment, the first sample dispensing component 400 includes a first sample needle 410, a first motion driving component and a first suction and discharge driving component, wherein the first motion driving component is used to drive the first sample needle 410 to move in two-dimensional or three-dimensional space, so as to move the first sample needle 410 to different positions, such as a standby position, a sample suction position, a sample addition position, a cleaning position, etc. The first suction and discharge driving component is used to drive the first sample needle 410 to perform suction and discharge actions.

[0067] As an implementation manner, the sample analyzer 10 further includes a standard solution dispensing component, and the standard solution dispensing component is used to dispense the standard solution into the detection container 200 .

[0068] As an implementation method, the standard solution dispensing component and the first sample dispensing component 400 are the same component, that is, the first sample dispensing component 400 is used not only to dispense samples but also to dispense standard solutions, which helps to reduce the cost of the sample analyzer 10. Of course, in a specific application, as an alternative implementation method, the standard solution dispensing component and the first sample dispensing component 400 can also be two independent components.

[0069] As an embodiment, the sample is loaded in a sample container, and the sample container is transported to the first sample suction position through a sample rack or a sample seat. The standard solution is loaded in a standard solution container, and the standard solution container is transported to the second sample suction position through a standard solution rack. The first sample suction position and the second sample suction position can be the same position or two different positions. The sample rack and the standard solution rack can be two different racks or the same rack. The first sample distribution component 400 is used to draw the sample from the sample container located at the first sample suction position and distribute it to the detection container 200, and is used to draw the standard solution from the standard solution container located at the second sample suction position and distribute it to the detection container 200.

[0070] As an embodiment, the sample analyzer 10 further includes an internal standard liquid distribution component 600, which is used to distribute the internal standard liquid into the detection container 200. The drainage component 300 is also used to drive a reference solution containing at least the internal standard liquid to flow through the electrode assembly 100. The controller controls the drainage component 300 to drive a calibration liquid to flow through the electrode assembly 100 and measure the corresponding first measurement parameter, including: controlling the drainage component 300 to drive a calibration liquid and a reference liquid to flow through the electrode assembly 100 respectively and measure the corresponding first measurement parameter. The controller controls the drainage component 300 to drive the liquid to be tested to flow through the electrode assembly 100 and obtain the corresponding second measurement parameter, including: controlling the drainage component 300 to drive the liquid to be tested and the reference liquid to flow through the electrode assembly 100 respectively and measure the corresponding second measurement parameter. The internal standard liquid is a liquid with a known target ion concentration and is configured inside the sample analyzer 10. Compared with the standard solution, the concentration of the target ion in the internal standard liquid will be closer to the target ion concentration in normal human serum. In the present embodiment, the second measurement parameter obtained by carrying out the electrolyte determination is obtained by the measurement liquid and the reference solution flowing through the electrode assembly 100 respectively, according to the electrical parameter calculation of the electrode assembly 100, so that the electrolyte determination result of the sample can be made more accurate, and the determination speed is faster. Since the reference solution containing the internal standard solution needs to flow through the electrode assembly 100 to obtain the second measurement parameter in the electrolyte determination of the sample, in order to make the calibration obtain the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter, and the correspondence used in the electrolyte determination for characterizing the relationship between the target ion concentration and the measurement parameter, so, the first measurement parameter obtained by the calibration process is obtained by the calibration solution and the reference solution flowing through the electrode assembly 100 respectively, according to the electrical parameter calculation of the electrode assembly 100. Of course, in specific applications, as an alternative embodiment, the reference solution containing the internal standard solution may not need to be used to flow through the electrode assembly 100 in the execution of the electrolyte determination and calibration.

[0071] As an embodiment, the internal standard liquid dispensing assembly 600 includes an internal standard liquid needle and a second suction and discharge driving component. The second suction and discharge driving component is used to drive the internal standard liquid needle to perform suction and discharge actions. The internal standard liquid needle is connected to the frame of the detection container 200 through a connecting seat. The internal standard liquid needle is immovable.

[0072] As an embodiment, the sample analyzer 10 also includes a diluent dispensing component 500, which is used to distribute the diluent into the detection container 200, the calibration solution is made of a standard solution and a diluent, the test solution is made of a sample and a diluent, and the reference solution is made of an internal standard solution and a diluent. In this embodiment, when performing electrolyte determination, the sample is first diluted with a diluent to obtain a test solution, and the target ion concentration in the sample is measured by an indirect method, which has the beneficial effects of consuming less sample volume and reducing the influence of substances such as proteins and lipids in the sample on the measurement results. Of course, in specific applications, as an alternative embodiment, the test solution may also only contain a sample without a diluent.

[0073] As an embodiment, the diluent dispensing assembly 500 includes a diluent needle, which is connected to the detection container 200 via a connecting seat. The diluent needle is immovable.

[0074] As an implementation mode, the first measurement parameter is the potential difference between the calibration solution flowing through the electrode assembly 100 and the reference solution flowing through the electrode assembly 100; the second measurement parameter is the potential difference between the test solution flowing through the electrode assembly 100 and the reference solution flowing through the electrode assembly 100. The first measurement parameter and the second measurement parameter use the potential difference, which is easy to implement.

[0075] As an embodiment, at least three calibration solutions include a first calibration solution containing at least a first standard solution, a second calibration solution containing at least a second standard solution, and a third calibration solution containing at least a third standard solution, wherein the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution and greater than the target ion concentration of the third standard solution. The first standard solution is a standard solution with a larger target ion concentration, the second standard solution is a standard solution with a smaller target ion concentration, the third standard solution and the second standard solution are standard solutions with a smaller target ion concentration than the internal standard solution, and the third standard solution is a standard solution with a smaller target ion concentration than the second standard solution. In this embodiment, a third standard solution with a lower target ion concentration is further added on the basis of the first standard solution and the second standard solution to calibrate the electrolyte determination, so that the target ion determination results of samples with a target ion concentration less than the concentration range of the first standard solution and the second standard solution can be reduced, and the accuracy of the electrolyte determination results of samples with small target ion concentrations can be improved.

[0076] As an embodiment, the third standard solution is a diluent, and the calibration solution containing at least the third standard solution is also a diluent. The target ion concentration of the diluent is very low and known. Using the diluent as a standard solution with a low target ion concentration, on the one hand, there is no need to add an additional third standard solution distribution component. On the other hand, since the diluent is used for calibration, it is close to zero point calibration, which effectively expands the target ion concentration range of the calibration, so that samples with a small target ion concentration also have an applicable calibration coefficient, which fully guarantees the accuracy and linearity of the electrolyte determination results of samples with a small target ion concentration.

[0077] As an implementation mode, the target ion concentration difference between the first standard solution and the second standard solution is greater than the target ion concentration difference between the second standard solution and the third standard solution, and the determined target ion concentration range of the first standard solution and the second standard solution is larger, which is conducive to fully ensuring that samples in a larger target ion concentration range can obtain accurate electrolyte measurement results through calibration results.

[0078] As an embodiment, the control liquid discharge component 300 drives at least three calibration liquids with different target ion concentrations to flow through the electrode assembly 100 and measure the corresponding first measurement parameters, and obtains a calibration result for characterizing the relationship between the target ion concentration and the measurement parameter according to the at least three first measurement parameters measured by the electrode assembly 100 for at least three calibration liquids, including: the control liquid discharge component 300 drives the first calibration liquid and the reference liquid containing at least the first standard solution to flow through the electrode assembly 100 and measure the first potential difference, the control liquid discharge component 300 drives the second calibration liquid and the reference liquid containing at least the second standard solution to flow through the electrode assembly 100 and measure the second potential difference, and the control liquid discharge component 300 drives the third calibration liquid and the reference liquid containing at least the third standard solution to flow through the electrode assembly 100 and measure the third potential difference, and obtains the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter according to at least the first potential difference, the second potential difference, and the third potential difference. In this embodiment, each standard solution is used for electrolyte measurement calibration, and the potential difference needs to be obtained by coordinating the measurement of the internal standard solution, which is conducive to ensuring the accuracy of the calibration result.

[0079] As an embodiment, according to at least three first measurement parameters measured by the electrode assembly 100 for at least three calibration solutions, a calibration result for characterizing the relationship between the target ion concentration and the measurement parameters is obtained, including: according to the two first measurement parameters measured by the electrode assembly 100 for any two calibration solutions with adjacent target ion concentrations, at least one of the following calibration results is obtained: a set of calibration parameters, a calibration straight line, and a calibration relationship. In this embodiment, any two calibration solutions with similar target ion concentrations are used as two points to obtain a calibration result, so that different calibration results can be used to calculate the target ion measurement results for samples in different concentration ranges, thereby ensuring that samples in different target ion concentration ranges can obtain relatively accurate measurement results.

[0080] As an embodiment, the electrode assembly 100 is used to measure at least two target ions in a test liquid or a calibration liquid, and obtains a calibration result for characterizing the relationship between the target ion concentration and the measurement parameter according to at least three first measurement parameters measured by the electrode assembly 100 for at least three calibration liquids, including: according to at least three first measurement parameters measured by the electrode assembly 100 for at least three calibration liquids, corresponding to each target ion, at least two groups of calibration parameters for a linear function characterizing the relationship between the target ion concentration and the measurement parameter are obtained, each group of calibration parameters includes a calibration slope and a calibration intercept of a linear function for characterizing the relationship between the target ion concentration and the measurement parameter, and the calibration slope and calibration intercept in each group of calibration parameters are obtained by two first measurement parameters measured by two calibration liquids with adjacent target ion concentrations, and the two calibration slopes of each group of calibration parameters are different. The target ion concentration and the measurement parameter satisfy the following linear function y=kx+b, wherein y is the measurement parameter, x is the target ion concentration, k is the slope, and b is the intercept. The calibration action is mainly to calibrate the slope k and intercept b in the linear function. In this embodiment, corresponding to each target ion concentration, at least two calibration slopes and calibration intercepts of linear functions can be obtained, and each linear function represents the relationship between the target ion concentration and the measurement parameter within the interval.

[0081] In the above scheme, the calibration result of each target ion measurement is the calibration slope and calibration intercept of at least two linear functions. Of course, in specific applications, the calibration result is not limited to this. For example, as an alternative embodiment, the calibration result of each target ion measurement can also be a calibration curve formed by splicing at least two calibration lines. Specifically, in this alternative embodiment, the electrode assembly 100 is used to measure at least two target ions in the test liquid or calibration liquid, and the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter is obtained according to at least three first measurement parameters measured by the electrode assembly 100 for at least three calibration liquids, including: according to the at least three first measurement parameters measured by the electrode assembly 100 for at least three calibration liquids, corresponding to each target ion, at least two calibration lines for characterizing the relationship between the target ion concentration and the measurement parameter are obtained, the at least two calibration lines obtained for each target ion are connected in sequence and have different slopes, and each calibration line is obtained by two first measurement parameters measured by two calibration liquids with adjacent target ion concentrations.

[0082] As an embodiment, the sample analyzer 10 pre-stores at least one of the following preset conditions: reaching the first preset time after the sample analyzer 10 is turned on; reaching the second preset time since the last calibration working mode; the time for the sample analyzer 10 to continuously perform the electrolyte determination working mode reaches the third preset time; the cumulative time for the sample analyzer 10 to perform the electrolyte determination working mode since the last calibration working mode is reached to the fourth preset time; the number of times the sample analyzer 10 continuously performs the electrolyte determination working mode reaches the first preset number of times; the number of times the sample analyzer 10 cumulatively performs the electrolyte determination working mode since the last calibration working mode is reached to the second preset number of times; reaching the preset time point of the preset cycle. The controller is also configured to: perform the calibration action when any preset condition is met. In this embodiment, the calibration action of the electrolyte determination will not take up the time of the electrolyte determination, and when the preset conditions are met, it is automatically executed according to the set program, which is conducive to making the operator basically unaware of the calibration action process of the electrolyte determination, and greatly reducing the interference of the calibration action process of the electrolyte determination on the operator.

[0083] As an implementation manner, the preset period is every day, every two days, every three days, every four days, every five days, or every week.

[0084] As an embodiment, the sample analyzer 10 further includes a reagent dispensing component, a second sample dispensing component and at least one optical measurement component, wherein the second sample dispensing component is used to draw a sample from a sample container and distribute it to a reaction container, the reagent dispensing component is used to draw a reagent from a reagent container and distribute it to a reaction container, and the optical measurement component is used to optically measure a reaction solution made of at least a sample distributed by the second sample dispensing component and a reagent distributed by the reagent dispensing component; at least one optical measurement component includes at least one of a biochemical measurement component, an immunoassay component, and a coagulation measurement component; wherein the second sample dispensing component and the first sample dispensing component 400 are the same component or two different components. In this embodiment, in addition to the integrated electrolyte measurement function, the sample analyzer 10 also integrates at least one of a biochemical measurement function, an immunoassay function, and a coagulation measurement function, that is, the electrolyte measurement module can be integrated in a biochemical analyzer or an immunoassay analyzer or a coagulation analyzer, which is conducive to diversifying the functions of the sample analyzer 10. Of course, in a specific application, as an alternative embodiment, the sample analyzer 10 can also have only an electrolyte measurement function, that is, the sample analyzer 10 can be a single electrolyte measurement machine.

[0085] As an implementation mode, the sample analyzer 10 is a biochemical analyzer integrated with electrolyte measurement.

[0086] This embodiment also provides a calibration method for electrolyte determination in a sample analyzer 10, the calibration method comprising the following steps: controlling the liquid discharge component 300 to drive at least three calibration solutions with different target ion concentrations to flow through the electrode component 100 respectively and measure the corresponding first measurement parameters; obtaining a calibration result for characterizing the relationship between the target ion concentration and the measurement parameter based on at least three first measurement parameters measured by at least three calibration solutions, the calibration result comprising at least one of a calibration parameter, a calibration curve, and a calibration relationship. This embodiment expands the calibration concentration range, allows low-concentration samples to have applicable calibration results, and improves the accuracy and linearity of the electrolyte determination results of low-concentration samples.

[0087] As an embodiment, the drainage component 300 is controlled to drive at least three calibration solutions with different target ion concentrations to flow through the electrode component 100 respectively and measure the corresponding first measurement parameters, including: controlling the drainage component 300 to drive a first calibration solution containing at least a first standard solution and a reference solution containing at least an internal standard solution to flow through the electrode component 100 respectively and measure a first potential difference, controlling the drainage component 300 to drive a second calibration solution containing at least a second standard solution and a reference solution containing at least an internal standard solution to flow through the electrode component 100 respectively and measure a second potential difference, and controlling the drainage component 300 to drive a third calibration solution containing at least a third standard solution and a reference solution containing at least an internal standard solution to flow through the electrode component 100 respectively and measure a third potential difference.

[0088] As an embodiment, the third standard solution and the calibration solution at least containing the third standard solution are both diluents.

[0089] As an embodiment, the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution and greater than the target ion concentration of the third standard solution.

[0090] The specific principles and implementations of the calibration method for electrolyte determination in the sample analyzer 10 provided in this embodiment are similar to the control principles and implementations described in the above sample analyzer 10 and will not be described in detail here.

[0091] This embodiment also provides a computer-readable storage medium, which stores a computer program. When the computer program is executed by a processor (such as the above-mentioned controller), the processor implements the steps of the calibration method for electrolyte determination in the above-mentioned sample analyzer 10. The computer-readable storage medium may be an internal storage unit of the above-mentioned sample analyzer 10, such as a hard disk or memory of the sample analyzer 10; or, the computer-readable storage medium may also be an external storage device of the sample analyzer 10, such as a plug-in hard disk, a smart memory card (SmartMedia Card, SMC), a secure digital (Secure Digital, SD) card, a flash card (Flash Card), etc. equipped on the sample analyzer 10.

[0092] As an implementation, the principle of the sample analyzer 10 includes:

[0093] (1) Use the ion selective electrode method (indirect method) to measure the target ion concentration in serum samples, plasma samples or urine samples. The measurement process is as follows: For example, measuring Na + / K + / Cl - During the test, the sample and the diluent are mixed at a certain dilution ratio. When the mixed test solution flows through the ion selective electrode 110 and the reference electrode 120, due to the characteristics of the electrode membrane, the target ions will undergo ion exchange and diffusion on the electrode membrane material. + / K + / Cl - The membrane potential of the ion selective electrode 110 forms a potential difference with the reference electrode 120. The response intensity of the membrane potential is related to the concentration of the target ion. The higher the concentration of the target ion, the greater the membrane potential. + / K + / Cl - The potential of the ion selective electrode 110 is related to the corresponding Na + / K + / Cl -The relationship between the ion concentration follows the Nernst equation. The potential of the test solution and the reference solution are measured to obtain the potential difference between the two. The concentration of the target ion in the sample can be calculated through the potential difference.

[0094] (2) In order to make the electrolyte determination results of samples with concentrations lower than the first standard solution and the second standard solution more accurate, this embodiment proposes a calibration method that adds zero point calibration, that is, a very low concentration calibration point (concentration lower than the linear range of the target ion project) is added on the basis of the first standard solution and the second standard solution to expand the target ion concentration range applicable to the calibration result, so that low target ion concentration samples have applicable calibration curves, and the accuracy and linearity of the low concentration sample determination results are improved. Because the target ion concentration of the diluent is very low and known, the diluent is used as the zero point calibration. The specific implementation method is: in the calibration process of the first standard solution and the second standard solution, an additional measurement of the diluent is added. According to the target ion concentration and measurement parameters measured by the diluent, combined with the measurement parameters measured by the second standard solution, a calibration curve suitable for low concentration samples is obtained, so that low concentration samples can also obtain more accurate results. During each calibration, the electrolyte module measures the first standard solution, the second standard solution and the diluent to obtain a standard first calibration straight line and a low value segment second calibration straight line (or obtains the slope and intercept of the first calibration straight line and the second calibration straight line). When measuring the sample, the corresponding calibration line (or the slope and intercept of the corresponding calibration line) is selected according to the potential difference obtained by the measurement, and the measurement result of the target ion is calculated. The present invention adds a zero point calibration on the basis of the existing calibration process, expands the calibration concentration range, allows low-concentration samples to have a suitable calibration curve, and improves the accuracy and linearity of the low-concentration sample results.

[0095] Embodiment 2:

[0096] The sample analyzer 10 and the calibration method for electrolyte determination in the sample analyzer 10 provided in this embodiment are different from those in the first embodiment mainly in that different standard solutions are used in the calibration process, which is specifically reflected in that: in the first embodiment, a third standard solution with a lower target ion concentration is further provided on the basis of the first standard solution and the second standard solution to calibrate the electrolyte determination; while in the present embodiment, a third standard solution with a lower target ion concentration and a fourth standard solution with a higher target ion concentration are further provided on the basis of the first standard solution and the second standard solution to calibrate the electrolyte determination.

[0097] Specifically, in this embodiment, at least three calibration solutions include a first calibration solution including at least a first standard solution, a second calibration solution including at least a second standard solution, a third calibration solution including at least a third standard solution, and a fourth calibration solution including at least a fourth standard solution, the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution and less than the target ion concentration of the fourth standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution and greater than the target ion concentration of the third standard solution. This embodiment allows samples with a smaller target ion concentration and a larger target ion concentration to have applicable calibration coefficients, fully ensuring the accuracy and linearity of the electrolyte determination results of samples with a smaller target ion concentration and samples with a larger target ion concentration.

[0098] As an embodiment, the third standard solution and the third calibration solution comprising the third standard solution are both diluents.

[0099] As an embodiment, the target ion concentration difference between the first standard solution and the second standard solution is greater than the target ion concentration difference between the second standard solution and the third standard solution, and the target ion concentration difference between the first standard solution and the second standard solution is greater than the target ion concentration difference between the fourth standard solution and the first standard solution.

[0100] Except for the above differences, other parts of the sample analyzer 10 and the calibration method for electrolyte determination in the sample analyzer 10 provided in this embodiment can refer to the first embodiment and will not be described in detail here.

[0101] Embodiment three:

[0102] The sample analyzer 10 and the calibration method for electrolyte determination in the sample analyzer 10 provided in this embodiment are different from those in the first embodiment mainly in that different standard solutions are used in the calibration process, which is specifically reflected in that: in the first embodiment, a third standard solution with a lower target ion concentration is further provided on the basis of the first standard solution and the second standard solution to calibrate the electrolyte determination; while in the present embodiment, a fourth standard solution with a higher target ion concentration is further provided on the basis of the first standard solution and the second standard solution to calibrate the electrolyte determination.

[0103] Specifically, in this embodiment, the at least three calibration solutions include a first calibration solution containing at least a first standard solution, a second calibration solution containing at least a second standard solution, and a fourth calibration solution containing at least a fourth standard solution, the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution and less than the target ion concentration of the fourth standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution. This embodiment can provide a sample with a larger target ion concentration with an applicable calibration coefficient, fully ensuring the accuracy and linearity of the electrolyte determination results of the sample with a larger target ion concentration.

[0104] Except for the above differences, other parts of the sample analyzer 10 and the calibration method for electrolyte determination in the sample analyzer 10 provided in this embodiment can refer to the first embodiment and will not be described in detail here.

[0105] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A sample analyzer, characterized in that: include: A detection container, wherein the detection container has an inner cavity and a liquid discharge port communicated with the inner cavity; a first sample dispensing component, the first sample dispensing component being used to dispense a sample into the inner cavity; An electrode assembly, the electrode assembly being used to measure target ions of a test liquid discharged from the liquid discharge port and containing at least a sample; A liquid discharge assembly, the liquid discharge assembly is used to drive the test liquid containing at least the sample to flow through the electrode assembly and to drive a calibration liquid containing at least a standard solution to flow through the electrode assembly; A controller, wherein the controller is configured to perform the following calibration actions: controlling the liquid discharge component to drive at least three calibration liquids with different target ion concentrations to flow through the electrode component respectively and measure corresponding first measurement parameters, and obtaining a calibration result for characterizing the relationship between the target ion concentration and the measurement parameter according to at least three first measurement parameters measured by the electrode component for the at least three calibration liquids, wherein the calibration result includes at least one of a calibration parameter, a calibration curve, and a calibration relationship; The controller is also configured to perform the following electrolyte measurement action: control the drainage component to drive the test liquid to flow from the detection container through the electrode component and obtain a corresponding second measurement parameter, and obtain the target ion measurement result of the sample based on the calibration result and the second measurement parameter measured by the electrode assembly for the test liquid.

2. The sample analyzer according to claim 1, wherein: The sample analyzer also includes an internal standard liquid dispensing component, which is used to dispense the internal standard liquid into the detection container; The liquid discharge assembly is also used to drive a reference solution containing at least the internal standard solution to flow through the electrode assembly; The controller controls the liquid discharge component to drive the calibration liquid to flow through the electrode component and measure the corresponding first measurement parameter, including: controlling the liquid discharge component to drive the calibration liquid and the reference liquid to flow through the electrode component respectively and measure the corresponding first measurement parameter; The controller controls the liquid discharge component to drive the liquid to be tested to flow through the electrode component and obtain the corresponding second measurement parameter, including: controlling the liquid discharge component to drive the liquid to be tested and the reference liquid to flow through the electrode component respectively and measuring the corresponding second measurement parameter.

3. The sample analyzer according to claim 2, wherein: The first measurement parameter is the potential difference between the calibration solution flowing through the electrode assembly and the reference solution flowing through the electrode assembly; The second measurement parameter is the potential difference between the test solution flowing through the electrode assembly and the reference solution flowing through the electrode assembly.

4. The sample analyzer according to claim 2 or 3, characterized in that: The at least three calibration solutions include a first calibration solution including at least a first standard solution, a second calibration solution including at least a second standard solution, and a third calibration solution including at least a third standard solution, wherein the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution and greater than the target ion concentration of the third standard solution.

5. The sample analyzer according to claim 4, characterized in that: The third standard solution and the calibration solution at least comprising the third standard solution are both diluents; and / or, The difference in target ion concentration between the first standard solution and the second standard solution is greater than the difference in target ion concentration between the second standard solution and the third standard solution.

6. The sample analyzer according to claim 4, characterized in that: The controlling the liquid discharge component to drive at least three calibration liquids with different target ion concentrations to flow through the electrode component respectively and measure corresponding first measurement parameters, and obtaining a calibration result for characterizing the relationship between the target ion concentration and the measurement parameter according to at least three first measurement parameters measured by the electrode component for the at least three calibration liquids, comprises: The drainage component is controlled to drive the first calibration solution and the reference solution, which at least include the first standard solution, to flow through the electrode component respectively and measure a first potential difference; the drainage component is controlled to drive the second calibration solution and the reference solution, which at least include the second standard solution, to flow through the electrode component respectively and measure a second potential difference; the drainage component is controlled to drive the third calibration solution and the reference solution, which at least include the third standard solution, to flow through the electrode component respectively and measure a third potential difference; and the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter is obtained at least according to the first potential difference, the second potential difference and the third potential difference.

7. The sample analyzer according to claim 2 or 3, characterized in that: The at least three calibration solutions include a first calibration solution including at least a first standard solution, a second calibration solution including at least a second standard solution, a third calibration solution including at least a third standard solution, and a fourth calibration solution including at least a fourth standard solution, the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution and less than the target ion concentration of the fourth standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution and greater than the target ion concentration of the third standard solution; or The at least three calibration solutions include a first calibration solution including at least a first standard solution, a second calibration solution including at least a second standard solution, and a fourth calibration solution including at least a fourth standard solution, the target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution and less than the target ion concentration of the fourth standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution.

8. The sample analyzer according to claim 7, wherein: The third standard solution and the third calibration solution prepared by including the third standard solution are both diluents; and / or, The target ion concentration difference between the first standard solution and the second standard solution is greater than the target ion concentration difference between the second standard solution and the third standard solution, and the target ion concentration difference between the first standard solution and the second standard solution is greater than the target ion concentration difference between the fourth standard solution and the first standard solution.

9. The sample analyzer according to claim 2 or 3, characterized in that: The sample analyzer also includes a diluent dispensing component, which is used to dispense diluent into the detection container, the calibration solution is made of the standard solution and the diluent, the test solution is made of the sample and the diluent, and the reference solution is made of the internal standard solution and the diluent.

10. The sample analyzer according to claim 1, wherein: The calibration result for characterizing the relationship between the target ion concentration and the measurement parameter is obtained based on at least three of the first measurement parameters measured by the electrode assembly on the at least three calibration solutions, including: obtaining at least one of the following calibration results based on two of the first measurement parameters measured by the electrode assembly on any two of the calibration solutions having adjacent target ion concentrations: a set of calibration parameters, a calibration straight line, and a calibration relationship.

11. The sample analyzer according to any one of claims 1 to 3 or 10, characterized in that: The electrode assembly is used to measure at least two target ions in the test liquid or the calibration liquid, and the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter is obtained according to the at least three first measurement parameters measured by the electrode assembly for the at least three calibration liquids, including: according to the at least three first measurement parameters measured by the electrode assembly for the at least three calibration liquids, corresponding to each target ion, at least two groups of calibration parameters of a linear function for characterizing the relationship between the target ion concentration and the measurement parameter are obtained, each group of the calibration parameters includes a calibration slope and a calibration intercept of a linear function for characterizing the relationship between the target ion concentration and the measurement parameter, the calibration slope and the calibration intercept in each group of the calibration parameters are obtained from two first measurement parameters measured by two calibration liquids with adjacent target ion concentrations, and the two calibration slopes of each group of the calibration parameters are different; and / or, The electrode assembly is used to measure at least two target ions in the test liquid or the calibration liquid, and the calibration result for characterizing the relationship between the target ion concentration and the measurement parameter is obtained based on the at least three first measurement parameters measured by the electrode assembly for the at least three calibration liquids, including: based on the at least three first measurement parameters measured by the electrode assembly for the at least three calibration liquids, corresponding to each target ion, at least two calibration lines for characterizing the relationship between the target ion concentration and the measurement parameter are obtained, the at least two calibration lines obtained for each target ion are connected in sequence and have different slopes, and each calibration line is obtained from two first measurement parameters measured by two calibration liquids with adjacent target ion concentrations.

12. The sample analyzer according to any one of claims 1 to 3 or 10, characterized in that: The sample analyzer pre-stores at least one of the following preset conditions: reaching a first preset time after the sample analyzer is turned on; reaching a second preset time since the calibration working mode was last executed; and reaching a third preset time after the sample analyzer continuously executes the electrolyte determination working mode. The cumulative time for the sample analyzer to execute the electrolyte determination working mode since the last execution of the calibration working mode reaches a fourth preset time; the number of times the sample analyzer continuously executes the electrolyte determination working mode reaches a first preset number; The number of times the sample analyzer has cumulatively executed the electrolyte determination working mode since the last execution of the calibration working mode reaches a second preset number; a preset time point of a preset period is reached, and the preset period is every day, every two days, every three days, every four days, every five days, or every week; The controller is further configured to: execute the calibration action when any one of the preset conditions is met.

13. The sample analyzer according to any one of claims 1 to 3 or 10, characterized in that: The sample analyzer further comprises a standard solution dispensing component, the standard solution dispensing component is used to dispense the standard solution into the detection container, the standard solution dispensing component and the first sample dispensing component are the same component or two independent components; and / or, The electrode assembly includes at least one ion selective electrode and a reference electrode. The drainage assembly drives the liquid to be tested in the detection container to flow through the electrode assembly, including driving the liquid to be tested in the detection container to flow through the ion selective electrode and the reference electrode in sequence. The drainage assembly drives the calibration liquid to flow through the electrode assembly, including driving the calibration liquid in the detection container to flow through the ion selective electrode and the reference electrode in sequence. The at least one ion selective electrode includes at least one of a sodium ion selective electrode, a chloride ion selective electrode, and a potassium ion selective electrode.

14. The sample analyzer according to any one of claims 1 to 3 or 10, characterized in that: The sample analyzer further comprises a reagent dispensing component, a second sample dispensing component and at least one optical measurement component, wherein the second sample dispensing component is used to draw a sample from a sample container and distribute it to a reaction container, the reagent dispensing component is used to draw a reagent from a reagent container and distribute it to the reaction container, and the optical measurement component is used to perform optical measurement on a reaction solution made of at least the sample dispensed by the second sample dispensing component and the reagent dispensed by the reagent dispensing component; The at least one optical assay component includes at least one of a biochemical assay component, an immunoassay component, and a coagulation assay component; The second sample distribution component and the first sample distribution component are the same component or two different components.

15. A calibration method for electrolyte determination in a sample analyzer, characterized in that: The steps include: Controlling the liquid discharge component to drive at least three calibration solutions with different target ion concentrations to flow through the electrode component respectively and measure corresponding first measurement parameters; According to the at least three first measurement parameters measured by the at least three calibration solutions, a calibration result for characterizing the relationship between the target ion concentration and the measurement parameters is obtained, and the calibration result includes at least one of a calibration parameter, a calibration curve, and a calibration relationship.

16. The calibration method for electrolyte determination in a sample analyzer according to claim 15, characterized in that: The control liquid discharge component drives at least three calibration solutions with different target ion concentrations to flow through the electrode component respectively and measure the corresponding first measurement parameters, including: The drainage component is controlled to drive the first calibration solution containing at least the first standard solution and the reference solution containing at least the internal standard solution to flow through the electrode component respectively and measure the first potential difference, the drainage component is controlled to drive the second calibration solution containing at least the second standard solution and the reference solution containing at least the internal standard solution to flow through the electrode component respectively and measure the second potential difference, and the drainage component is controlled to drive the third calibration solution containing at least the third standard solution and the reference solution containing at least the internal standard solution to flow through the electrode component respectively and measure the third potential difference.

17. The calibration method for electrolyte determination in a sample analyzer according to claim 16, characterized in that: The third standard solution and the calibration solution at least comprising the third standard solution are both diluents; and / or, The target ion concentration of the first standard solution is greater than the target ion concentration of the internal standard solution, and the target ion concentration of the second standard solution is less than the target ion concentration of the internal standard solution and greater than the target ion concentration of the third standard solution.

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