Pesticide composition containing Lubao No.1 and used for preventing and treating semen cuscutae

Abstract

The invention belongs to the technical field of agricultural weed control, and provides a pesticide composition containing 'Lubao No.1' for controlling semen cuscutae, and the composition also contains an effective component clomazone. The ratio of clomazone to 'Lubao No.1' is (2-8) g: 10 < 9 > cfu. When the composition is used for preventing and treating semen cuscutae in soybeans, the use dosage of clomazone is 144-360g a.i. / hm < 2 >. The screened agent composition is good in safety to soybeans and excellent in semen cuscutae prevention and removal effect, the short-term prevention effect can be improved, and the use amount of chemical agents can be reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural weed control and prevention, and relates to a biological agent combination for controlling dodder. Background Art

[0002] The disclosure of this background information is intended to enhance understanding of the general background of the invention and should not necessarily be taken as an acknowledgment or any form of suggestion that this information constitutes the prior art already known to a person skilled in the art.

[0003] Cuscuta is a plant of the genus Cuscuta in the family Convolvulaceae. Cuscuta sp. ) is a common name for plants that are a type of malignant parasitic weed that causes serious damage. Cuscuta absorbs nutrients from the host through a special organ called a "haustorium". Cuscuta cannot survive without the host plant. Therefore, after a long period of evolution and rapid spread, Cuscuta can now parasitize more than a thousand plants. Cuscuta is widely distributed in Heilongjiang, Gansu, Shandong, Jiangsu, Hunan, Hubei, Jiangxi, Guangdong and other provinces in my country, posing a serious threat to the growth of many crops.

[0004] Chemical herbicides have made important contributions to preventing and controlling weeds in farmland, increasing crop yields, and ensuring food security. However, dodder has strong vitality and is prone to form a close parasitic relationship with the host. The incorrect application of chemical herbicides can easily cause phytotoxicity to the host plant. Colletotrichum gloeosporioide Penz. Sacc. f. sp.cuscuta) is a microbial agent with special control effect on dodder, but the application of this microbial agent is affected by the use time and application environment, and the control effect is prone to fluctuation. The combination of the above factors has resulted in the lack of a technical model that can effectively control dodder. Summary of the invention

[0005] In view of the problem of lack of dodder control agents and technologies, the present invention provides a pesticide composition for controlling dodder containing "Lubao No. 1", which adopts a combination of biological pesticides and chemical pesticides to effectively control dodder damage in soybean fields, greatly improve the control effect and extend the application time.

[0006] To achieve the above purpose, the present invention adopts the following technical solution.

[0007] A pesticide composition for controlling dodder containing "Lubao No. 1" comprises clomazone and "Lubao No. 1".

[0008] The ratio of clomazone to "Lubao No. 1" is (2-8) g:10 9 cfu.

[0009] The crops controlled by the pesticide composition are Cuscuta spp. ( CuscutaLinn.) plants; preferably Cuscuta chinensis ( Cuscuta chinensis Lam.), Cuscuta australis ( Cuscuta australis R. Br.).

[0010] The application object of the pesticide composition is a soybean field.

[0011] The above composition may also contain other inert ingredients for stabilizing the active ingredients and facilitating their application to crops or attachment to targets, such as fillers, wetting agents, dispersants, defoamers, antifreeze agents, solvents, emulsifiers, preservatives, disintegrants, etc.

[0012] The above composition can be processed by the common methods in the prior art, and the preferred dosage forms include suspensions, oil suspensions, microcapsule suspensions, wettable powders, water-dispersible granules, emulsifiable concentrates, and microemulsions.

[0013] A method for controlling dodder in soybean fields comprises the following steps: mixing clomazone and "Lubao No. 1" to spray the stems and leaves of dodder; The ratio of clomazone to "Lubao No. 1" is (2-8) g:10 9 cfu; the dosage of clomazone is 144g / hm 2 -360g / hm 2 .

[0014] Preferably, the dosage of "Lubao No. 1" is (4.5-6.75)×10 10 cfu / hm 2 .

[0015] A method for controlling dodder in soybean fields comprises the following steps: (1) In the early stage of dodder parasitism, use "Lubao No. 1" at 6.75×10 10 cfu / hm 2 Stem and leaf spray; (2) During the secondary parasitic period of dodder, use clomazone at an effective ingredient of 144-360 g / hm2. 2 Spray the stems and leaves, and after 3-5 days, spray "Lubao No. 1" at 6.75×10 10 cfu / hm 2 Spray the stems and leaves.

[0016] (3) During the period of widespread dodder damage, the “Lubao No. 1” microbial agent was used at 6.75×10 10 cfu / hm 2 and clomazone 144-360 g ai / hm 2 Mix and spray, and use "Lubao No. 1" fungicide at 4.5×1010 cfu / hm 2 Apply stem and leaf spray.

[0017] The initial stage of parasitism is when the dodder only parasitizes the original plant; the secondary parasitic stage is when the dodder climbs from the original parasitic plant to the adjacent plant; and the large-area damage stage is when the dodder has completed multiple levels of secondary parasitism.

[0018] The endpoints and any values ​​of the ranges disclosed in this application are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be regarded as specifically disclosed in this application.

[0019] The present invention has the following advantages: Currently, the market lacks highly effective and safe agents for preventing and controlling dodder. The stability of the efficacy of the "Lubao No. 1" bacterial agent is restricted by environmental conditions. The present invention aims at the shortcomings of the use of the "Lubao No. 1" bacterial agent and specifically screens highly effective agents for complementary application, thereby achieving the purpose of ensuring the use period and use environment of biological agents, reducing the potential phytotoxicity of chemical agents, reducing the application of chemical agents, and effectively reducing the harm of dodder. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a picture of dodder damaging soybeans; Figure 2 This is a picture of the control of Chinese dodder in soybean fields using "Lubao No. 1" and isothiocarp. DETAILED DESCRIPTION

[0021] The present invention will be further described below in conjunction with examples, but the present invention is not limited by the following examples.

[0022] Example 1 Screening of highly effective dodder control agents 1. Single-dose screening Test plots: Soybean fields with serious dodder infestation were selected.

[0023] There were 15 herbicides in total, including quizalofop-p-ethyl, sethoxydim, 2,4-D isooctyl, promethazine, triclopyr, fomesafen, fluazifop-butyl, bentazon, trifluorfen, butalin, S-isopropylamine, fluazifop-butyl, imazethapyr, isothiocarb, and quinclorac; all the herbicides were commercially purchased preparations.

[0024] Dosage and method of use: The lowest recommended field dose as indicated on the pesticide formulation label; Use ASS-4 automatic control spraying system, fan-shaped nozzle, spray pressure 0.4MPa, nozzle flow 800mL / min, adjust the operating speed according to the actual spraying area, and spray evenly.

[0025] Results survey: On the 15th day after the application of the drug, three random points were surveyed in each plot, and the status of 20 dodder vines were randomly surveyed in the selected area, the survival rate was recorded, and the control effect was calculated. The calculation formula is as follows: Control effect (%) = (number of surviving dodder vines in the control area - number of surviving dodder vines in the treatment area) / number of surviving dodder vines in the control area × 100.

[0026] Randomly investigate the growth status of 100 crops and record the pesticide damage to soybean according to the following standards: Level 0: Plants grow normally, no pesticide damage; Level I: The crops are slightly damaged by pesticides, with the degree of damage being less than 10%; Level II: moderate damage to crops, which can be recovered later; Level III: Crops are severely damaged by pesticides and are difficult to recover or even die.

[0027] Table 1 The control effect of different pesticides on dodder and their safety on soybean Note: “-” is invalid and “ / ” is not counted.

[0028] As can be seen from Table 1, the 15 selected herbicides had quite different effects on dodder control, but 100% effective herbicides all had devastating effects on the host soybean. In subsequent experiments, reducing the herbicide concentration (diluting it 5 times) still failed to effectively reduce the occurrence of phytotoxicity.

[0029] 2. Screening of special control agents for Cuscuta The formula was screened by adding 480 g / L of clomazone EC purchased from the market to the spore solution of "Lubao No. 1". The use concentration of "Lubao No. 1" was fixed at 6.75×10 10 cfu / hm 2 The main dodder species that cause harm is Dodder sinensis ( Cuscuta chinensis Lam.).

[0030] Three soybean fields were selected for experiments according to the degree and period of dodder damage: (a) Initial stage of parasitism: dodder only parasitizes the original plant; (b) Secondary parasitic stage: Cuscuta climbs from the original parasitic plant to the adjacent plant, showing a trend of rapid spread; (c) Large-scale damage period: Cuscuta has completed multiple levels of secondary parasitism.

[0031] Use ASS-4 automatic control spraying system, fan-shaped nozzle, spray pressure 0.4MPa, nozzle flow 800mL / min, adjust the operating speed according to the actual spraying area, and spray evenly.

[0032] Results survey: On the 15th day after the application of the drug, three random points were surveyed in each plot, and the status of 20 dodder vines were randomly surveyed in the selected area, the survival rate was recorded, and the control effect was calculated. The calculation formula is as follows: Control effect (%) = (number of surviving dodder vines in the control area - number of surviving dodder vines in the treatment area) / number of surviving dodder vines in the control area × 100.

[0033] Table 2 The control effect of “Lubao No. 1” and different doses of clomazone on dodder It can be seen from Table 2 that the mixture of "Lubao No. 1" and isothiocarp has a good effect on Cuscuta chinensis, and the combined effect is an obvious synergistic effect; and the combined use reduces the instability of "Lubao No. 1" when used alone, reduces the impact of environmental factors on the efficacy of "Lubao No. 1", ensures the use period and expands the use environment, and achieves a good complementary effect.

[0034] Example 2: Mixing of "Lubao No. 1" and clomazone to control dodder in soybean fields According to the method in Example 1, in different Chinese dodder seeds ( Cuscuta chinensis Field tests were conducted on soybean fields with the same damage level as Lam., and the results were checked 15 and 30 days after the last application: (1) In the early stage of dodder infection, dodder only parasitizes the original plant, and the dodder coverage area is less than 20%; a) Using clomazone 144 g ai / hm 2 Spraying, evenly spray the stems and leaves between 9-10 am, with a liquid volume of 30 L per mu; on the 6th day after spraying, use Lubao No. 1 fungicide for secondary prevention and control at around 4 pm, with a spore liquid concentration of 1.0×10 8 / L, the amount of liquid medicine per mu is 30 L; b) The “Lubao No. 1” fungicide was used for prevention and control. The primary conidia liquid was evenly sprayed at around 4 pm. The concentration of the conidia liquid used was 1.5×10 8 / L, the amount of liquid medicine per mu is 30 L; (2) After the dodder has completed secondary parasitism, it climbs from the original parasitic plant to the adjacent plant, showing a spreading trend, and the dodder covers about 50% of the area; a) Using clomazone 252 g ai / hm 2Spraying, evenly spray the stems and leaves between 9-10 am, with a liquid volume of 30 L per mu; on the 6th day after spraying, use Lubao No. 1 fungicide for secondary prevention and control at around 4 pm, with a spore liquid concentration of 1.0×10 8 / L, the amount of liquid medicine per mu is 30 L; b) Using Lubao No. 1 bacterial agent + isothiocarb 252 g ai / hm 2 The mixed agent was evenly sprayed on the stems and leaves at around 4 pm. The spore solution concentration was 1.5×10 8 / L, the amount of liquid medicine per mu is 30 L; c) Using clomazone 252 g ai / hm 2 Spraying: spray evenly on stems and leaves between 9-10 am, with 30 L of liquid per mu; c) Use Lubao No. 1 fungicide to spray stems and leaves at around 4 pm. The spore concentration used is 1.5×10 8 / L, the liquid volume per mu was 30 L; on the 6th day after spraying, Lubao No. 1 fungicide was used for secondary prevention and control at around 4 pm, and the spore liquid concentration used was 1.0×10 8 / L, the amount of liquid medicine per mu is 30 L; (3) During the period of large-scale damage caused by dodder, dodder has completed multiple secondary parasitic processes and has caused large-scale damage, with dodder covering about 80% of the area; a) Using Lubao No. 1 bacterial agent + isothiocarb 252 g ai / hm 2 The mixed agent was evenly sprayed on the stems and leaves at around 4 pm. The spore solution concentration was 1.5×10 8 / L, the liquid volume per mu was 30 L; on the fourth day after spraying, Lubao No. 1 fungicide was used for secondary prevention and control at around 4 pm, and the spore liquid concentration used was 1.0×10 8 / L, the amount of liquid medicine per mu is 30 L; b) Using clomazone 252 g ai / hm 2 Spray evenly on stems and leaves between 9-10 am, with 30 L of liquid per mu.

[0035] Table 3 The control effect of mixed use of "Lubao No. 1" and isothiocarp on dodder with different damage degrees From the data in Table 3, we can see that for fields with less damage from dodder, "Lubao No. 1" can be used alone for control. Although the control effect is poor 15 days after application, the long-term control effect is not significantly different from that of the single agent control. When dodder is in a secondary parasitic state or a large-scale damage state, the effect of combined control of "Lubao No. 1" and clomazone is significantly better than that of clomazone alone in both the short and long term. When dodder is in the secondary parasitism stage, the long-term effect of using "Lubao No. 1" and isothiocarb separately is better than using the two in a tank mixture.

[0036] The above is only an embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims

1. A pesticide composition for controlling dodder containing "Lubao No. 1", characterized in that: Including clomazone and Lubao One; The ratio of clomazone to "Lubao No. 1" is (2-8) g:10 9 cfu; The application object of the pesticide composition is a soybean field.

2. The pesticide composition according to claim 1, characterized in that The control target of the pesticide composition is Cuscuta spp. ( Cuscuta Linn.) plants.

3. The pesticide composition according to claim 1, characterized in that The control target of the pesticide composition is Chinese dodder seed ( Cuscuta chinensis Lam.) and Cuscuta australis ( Cuscuta australis R. Br.).

4. The pesticide composition according to claim 1, characterized in that The pesticide composition further comprises other inert ingredients, wherein the inert ingredients are selected from at least one of fillers, wetting agents, dispersants, defoamers, antifreeze agents, solvents, emulsifiers, preservatives and disintegrants.

5. The pesticide composition according to claim 1, characterized in that: The dosage form is selected from suspension, oil suspension, microcapsule suspension, wettable powder, water dispersible granules, emulsifiable concentrate or microemulsion.

6. A method for controlling dodder in soybean fields, characterized in that: The following steps are involved: Mix clomazone and "Lubao No. 1" and spray the stems and leaves of Cuscuta australis; the ratio of clomazone to "Lubao No. 1" is (3.2-8) g:10 8 cfu; the dosage of clomazone is 144g / hm 2 -360g / hm 2 .

7. The method according to claim 6, characterized in that the dosage of "Lubao No. 1" is (4.5-6.75)×10 10 cfu / hm 2 .

8. A method for controlling dodder in soybean fields, characterized in that: The following steps are involved: (1) In the early stage of dodder parasitism, use "Lubao No. 1" at 6.75×10 10 cfu / hm 2 Stem and leaf spray; (2) During the secondary parasitic period of dodder, use clomazone at an effective ingredient of 144-360 g / hm2. 2 Spray the stems and leaves, and after 3-5 days, spray "Lubao No. 1" at 4.5×10 10 cfu / hm 2 Spray the stems and leaves. (3) During the period of widespread dodder damage, the use of "Lubao No. 1" microbial agent 6.75×10 10 cfu / hm 2 and clomazone 144-360 g / hm 2 Mix and spray, and use "Lubao No. 1" fungicide at 4.5×10 10 cfu / hm 2 Apply stem and leaf spray; The initial stage of parasitism is when the dodder only parasitizes the original plant; the secondary parasitic stage is when the dodder climbs from the original parasitic plant to the adjacent plant; and the large-area damage stage is when the dodder has completed multiple levels of secondary parasitism.

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