Oyster peptide composition capable of improving sleep and preparation method thereof

Abstract

The invention relates to the technical field of health food preparation, and particularly discloses an oyster peptide composition capable of improving sleep and a preparation method thereof, the oyster peptide composition is prepared from 50-60 parts of oyster peptide powder, 10-15 parts of gamma-aminobutyric acid GABA, 15-20 parts of spina date seed extract, 0.3-0.5 part of melatonin, 5-8 parts of vitamin B6, 10-15 parts of lycium barbarum polysaccharide and 8-10 parts of functional probiotic powder; the finished product oyster peptide composition prepared by the preparation method disclosed by the invention is high in safety of natural components, and the oyster peptide is rich in micromolecule active peptide, taurine, zinc, selenium and other microelements and has multiple sleep aiding mechanisms; neurotransmitters can be adjusted, and active peptides can promote GABA secretion, relieve anxiety and enhance the sedation effect; the traditional Chinese medicine composition can improve the biological clock, improve the serum melatonin level, help to adjust the circadian rhythm, promote deep sleep, adjust the function of a central nervous system and increase the deep sleep proportion, and has the functions of resisting oxidation and relieving fatigue.

Description

Technical Field

[0001] The invention belongs to the technical field of health food preparation, and particularly relates to an oyster peptide composition capable of improving sleep and a preparation method thereof. Background Art

[0002] In the fast-paced modern life, sleep disorders have become a common problem that plagues a large number of people. At present, although there are many products on the market to improve sleep, such as melatonin and sleeping pills, these products often have disadvantages such as large side effects, strong dependence or single effect. For example, sleeping pills may cause mental fatigue and memory loss the next day; and although melatonin can help regulate sleep to a certain extent, its mechanism of action is relatively simple and has limited effect on sleep disorders caused by anxiety, stress and other factors.

[0003] Most of the sleep-improving products in the existing technology focus on a single ingredient or mechanism, lacking a comprehensive consideration of sleep problems. At the same time, the chemical ingredients in some products may have adverse effects on users, and long-term use may bring health risks. Therefore, it is particularly important to develop a safe, effective and comprehensive sleep-improving product.

[0004] In view of this, the inventors proposed an oyster peptide composition having the effect of improving sleep and a preparation method thereof to solve the above problems. Summary of the invention

[0005] The object of the present invention is to provide an oyster peptide composition having the function of improving sleep and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] An oyster peptide composition having the function of improving sleep, comprising:

[0008] 50-60 parts of oyster peptide powder, 10-15 parts of gamma-aminobutyric acid GABA, 15-20 parts of spiny jujube kernel extract, 0.3-0.5 parts of melatonin, 5-8 parts of vitamin B6, 10-15 parts of wolfberry polysaccharides and 8-10 parts of functional probiotic powder.

[0009] Preferably, the oyster peptide composition consists of the following ingredients:

[0010] 50 parts of oyster peptide powder, 10 parts of gamma-aminobutyric acid GABA, 15 parts of spiny jujube kernel extract, 0.3 parts of melatonin, 5 parts of vitamin B6, 10 parts of wolfberry polysaccharides and 8 parts of functional probiotic powder.

[0011] A method for preparing an oyster peptide composition having the function of improving sleep comprises the following steps:

[0012] S1. Raw material processing: select high-quality oysters, clean and shell them, and take the meat part;

[0013] The oyster meat is enzymolyzed with a composite protease, 0.2% calcium ions are added during the reaction, and an enzymolysis solution is obtained after the enzymolysis is completed;

[0014] The enzymatic hydrolysate is heated to 90° C. and maintained for 15 minutes to inactivate the enzyme;

[0015] After the enzyme is inactivated, the residue is separated from the enzymatic hydrolyzate to obtain a clear solution;

[0016] S2, concentration and drying: concentrating the clear liquid under vacuum conditions to a solid content of 20%, and drying to obtain oyster peptide powder;

[0017] S3, composition mixing: oyster peptide powder, γ-aminobutyric acid, jujube seed extract, and wolfberry polysaccharide are added into a mixer according to the formula ratio and mixed evenly, melatonin and vitamin B6 are added, and mixed at a low speed for 10 minutes to avoid inactivation of functional ingredients, and finally functional probiotic powder is added and mixed evenly under low temperature conditions;

[0018] S4. Particle size screening: remove uneven particles through an 80-mesh sieve to obtain a finished oyster peptide composition.

[0019] Preferably, the enzymatic hydrolysis is carried out at a temperature of 50-55° C., a pH of 7.0, and a time of 6 hours.

[0020] Preferably, the drying equipment is a spray dryer, the air inlet temperature of the spray dryer is 150°C, and the air outlet temperature is 80°C.

[0021] Preferably, the vacuum degree of the vacuum condition is 10 -3 Pa~10 -2 Pa.

[0022] Preferably, the mixing condition parameters of the mixer are: temperature 10-20° C., stirring speed 150-220 rpm, and time 15-20 min.

[0023] Preferably, the low temperature condition is 15-25° C., and the mixing time is 15-20 min.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] (1) The finished oyster peptide composition prepared by the present invention has high natural ingredient safety. Oyster peptides are rich in small molecule active peptides, taurine, zinc, selenium and other trace elements, and have multiple sleep-aiding mechanisms. They can regulate neurotransmitters, and active peptides can promote GABA secretion, relieve anxiety, and enhance sedative effects. They can improve the biological clock, increase serum melatonin levels, and help regulate circadian rhythms.

[0026] (2) The oyster peptide composition of the present invention can promote deep sleep, regulate the function of the central nervous system, increase the proportion of deep sleep, and has antioxidant and fatigue-relieving functions. Elements such as zinc and selenium participate in the antioxidant reaction, which helps to alleviate sleep problems caused by fatigue. Experimental results show that the oyster peptide composition can not only shorten the time to fall asleep, but also increase the proportion of deep sleep, reduce the number of awakenings at night, and improve the mental state after waking up, thereby optimizing the entire sleep cycle. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The present invention is a flow chart of a method for preparing an oyster peptide composition having the function of improving sleep. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Embodiment 1:

[0030] See also Figure 1 As shown, an oyster peptide composition for improving sleep is composed of the following ingredients: 50 parts of oyster peptide powder, 10 parts of γ-aminobutyric acid GABA, 15 parts of spiny jujube kernel extract, 0.3 parts of melatonin, 5 parts of vitamin B6, 10 parts of wolfberry polysaccharides and 8 parts of functional probiotic powder.

[0031] A method for preparing an oyster peptide composition having the function of improving sleep comprises the following steps:

[0032] S1. Raw material processing: select high-quality oysters, clean and shell them, and take the meat part;

[0033] The oyster meat was enzymolyzed with a composite protease at 50°C and pH 7.0 for 6 hours, 0.2% calcium ions were added during the enzymolysis reaction to improve the enzymolysis efficiency, and an enzymolysis solution was obtained after the enzymolysis was completed;

[0034] The enzymatic hydrolysate is heated to 90° C. and maintained for 15 minutes to inactivate the enzyme;

[0035] After the enzyme is inactivated, the residue is separated from the enzymatic hydrolyzate to obtain a clear solution;

[0036] S2, concentration and drying: the clear liquid is concentrated using a vacuum concentrator at a vacuum degree of 10 -3The mixture was concentrated to a solid content of 20% under the conditions of Pa, and dried using a spray dryer with the inlet air temperature set at 150°C and the outlet air temperature set at 80°C to obtain oyster peptide powder;

[0037] S3, composition mixing: oyster peptide powder, γ-aminobutyric acid, jujube seed extract, and wolfberry polysaccharide are added into a V-type mixer according to the formula ratio, and mixed evenly at a temperature of 15°C, a stirring speed of 150 rpm, and a time of 20 min. Melatonin and vitamin B6 are added, and mixed at a low speed for 15 minutes under the same conditions to avoid inactivation of functional ingredients. Finally, functional probiotic powder is added and mixed evenly;

[0038] S4. Particle size screening: Use an 80-mesh sieve to remove uneven particles to ensure a delicate taste and obtain a finished oyster peptide composition.

[0039] Finally, the finished oyster peptide composition is packaged in an aluminum foil bag lined with a food-grade polyethylene bag;

[0040] Environmental requirements for packaging: nitrogen protection (oxygen content ≤ 1%), humidity ≤ 40%, temperature ≤ 20°C.

[0041] Sealing method: Sealed with a heat sealer to ensure no air leakage.

[0042] Directions: Take 3 grams daily with warm water 30 minutes before bedtime.

[0043] As can be seen from the above, the finished oyster peptide composition prepared by the present invention has high natural ingredient safety. Oyster peptides are rich in small molecule active peptides, taurine, zinc, selenium and other trace elements, and have multiple sleep-aiding mechanisms:

[0044] It can regulate neurotransmitters, and active peptides can promote GABA secretion, relieve anxiety and enhance sedative effects.

[0045] It can improve the biological clock, increase serum melatonin levels, and help regulate circadian rhythms.

[0046] It can promote deep sleep, regulate the function of the central nervous system, and increase the proportion of deep sleep.

[0047] It has antioxidant and fatigue-relieving functions. Elements such as zinc and selenium participate in the antioxidant reaction, which helps to relieve sleep problems caused by fatigue.

[0048] Experimental results show that the oyster peptide composition can not only shorten the time to fall asleep, but also increase the proportion of deep sleep, reduce the number of awakenings at night, and improve the mental state after waking up, thereby optimizing the entire sleep cycle.

[0049] Embodiment 2:

[0050] Application Example: Study on the Effect of Improving Sleep

[0051] Experimental background: To verify the effect of oyster peptide composition on improving sleep quality.

[0052] Participants: 40 voluntary adults aged 30-60 years who reported difficulty falling asleep, waking up easily during the night, or poor sleep quality.

[0053] Experimental design

[0054] Grouping

[0055] Experimental group (20 people): Take 3 grams of oyster peptide composition 30 minutes before going to bed every night, washed down with warm water.

[0056] Control group (20 people): took the same dose of placebo (corn starch).

[0057] Observation period

[0058] The study lasted for 8 weeks, with assessments every 2 weeks.

[0059] Evaluation Metrics

[0060] Subjective sleep quality (Pittsburgh Sleep Quality Index, PSQI): 7 items are scored, and the lower the total score, the better the sleep quality.

[0061] Polysomnography (PSG): including sleep onset latency (SOL), deep sleep ratio (DS), and number of awakenings at night.

[0062] Biochemical indicators: Determine the changes in serum GABA and melatonin levels before and after the experiment.

[0063] Detailed data

[0064] 1. Subjective sleep quality (PSQI score), as shown in Table 1 below:

[0065]

[0066]

[0067] Table 1

[0068] The PSQ I score of the experimental group was significantly reduced, indicating that the subjective sleep quality was significantly improved.

[0069] 2. Polysomnography (PSG data), as shown in Table 2 below:

[0070]

[0071]

[0072] Table 2

[0073] The experimental group performed significantly better than the control group in terms of shortened sleep onset time, increased deep sleep ratio and reduced number of nighttime awakenings.

[0074] 3. Changes in biochemical indicators, as shown in Table 3 below:

[0075]

[0076] Table 3

[0077] The levels of GABA and melatonin in the serum of the experimental group were significantly increased, indicating that the composition effectively regulates the central nervous system and the biological clock.

[0078] Summary of the results

[0079] Subjective feelings:

[0080] 80% of the subjects in the experimental group reported significantly improved sleep quality, falling asleep faster and fewer awakenings during the night.

[0081] Objective monitoring:

[0082] The experimental group's sleep onset time was shortened by 43.3%, the deep sleep ratio was increased by 55.9%, and the number of nighttime awakenings was reduced by 50%.

[0083] Safety and Tolerability:

[0084] No serious adverse reactions were observed, and some subjects reported occasional mild stomach bloating, which usually disappeared after 2-3 days of taking the drug.

[0085] Implementation recommendations

[0086] Target population: Suitable for people with sleep disorders caused by stress or biological clock disorders, especially white-collar workers and the elderly who are in sub-healthy conditions.

[0087] Dosage: 30 minutes before bedtime, take 3 grams of powder and mix it with 150 mL of warm water, stir well and take.

[0088] Supporting measures: It is recommended to combine healthy work and rest habits and relaxation training to enhance the overall effect.

[0089] From the above, we can see that the oyster peptide composition not only has a significant effect in improving sleep quality, but also can achieve a multi-dimensional sleep-aiding effect by increasing serum GABA and melatonin levels, while having good safety and tolerability.

[0090] Embodiment three:

[0091] This design is specifically applied in the medical field. The oyster peptide composition can be used as an auxiliary treatment to improve the sleep quality of patients with sleep disorders. In particular, for sleep disorders caused by anxiety, stress, biological clock disorders and other factors, the oyster peptide composition can play a significant regulatory role. In addition, its antioxidant and fatigue-relieving functions can also help patients restore their physical strength and improve their quality of life.

[0092] Furthermore, compared with traditional sleep aid products (melatonin single preparation)

[0093] Experimental background

[0094] Objective: To evaluate the effects of a combination of oyster peptides and melatonin alone on improving sleep quality. Subjects: 40 participants, aged 30–60 years, reporting sleep disturbances.

[0095] Experimental design

[0096] Grouping

[0097] Experimental group (20 people): took oyster peptide composition, 3 grams 30 minutes before bedtime every day.

[0098] Control group (20 people): took a single preparation containing 3 mg of melatonin (common market dose).

[0099] Observation period

[0100] This lasted for 8 weeks, with assessments every 2 weeks.

[0101] Evaluation Metrics

[0102] Subjective sleep quality (PSQ I score).

[0103] Polysomnography (PSG): time to fall asleep, proportion of deep sleep, number of awakenings at night.

[0104] Changes in serum melatonin levels.

[0105] Detailed data comparison is shown in Table 4

[0106]

[0107] Table 4

[0108] From the above, it can be seen that the effect of the oyster peptide combination is significantly better than that of the single melatonin preparation, especially in terms of increasing the proportion of deep sleep and reducing nighttime awakenings.

[0109] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine different embodiments or examples described in this specification and the features of different embodiments or examples, unless they are contradictory.

[0110] In the drawings of the embodiments disclosed in the present invention, only the structures related to the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0111] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An oyster peptide composition having the function of improving sleep, characterized in that: The proportion by weight includes: 50-60 parts of oyster peptide powder, 10-15 parts of gamma-aminobutyric acid GABA, 15-20 parts of spiny jujube kernel extract, 0.3-0.5 parts of melatonin, 5-8 parts of vitamin B6, 10-15 parts of wolfberry polysaccharides and 8-10 parts of functional probiotic powder.

2. The oyster peptide composition for improving sleep according to claim 1, characterized in that: The oyster peptide composition consists of the following ingredients: 50 parts of oyster peptide powder, 10 parts of gamma-aminobutyric acid GABA, 15 parts of spiny jujube kernel extract, 0.3 parts of melatonin, 5 parts of vitamin B6, 10 parts of wolfberry polysaccharides and 8 parts of functional probiotic powder.

3. A method for preparing an oyster peptide composition having the function of improving sleep according to any one of claims 1 to 2, characterized in that: The following steps are involved: S1. Raw material processing: select high-quality oysters, clean and shell them, and take the meat part; The oyster meat is enzymolyzed with a composite protease, 0.2% calcium ions are added during the reaction, and an enzymolysis solution is obtained after the enzymolysis is completed; The enzymatic hydrolysate is heated to 90° C. and maintained for 15 minutes to inactivate the enzyme; After the enzyme is inactivated, the residue is separated from the enzymatic hydrolyzate to obtain a clear solution; S2, concentration and drying: concentrating the clear liquid under vacuum conditions to a solid content of 20%, and drying to obtain oyster peptide powder; S3, composition mixing: oyster peptide powder, γ-aminobutyric acid, jujube seed extract, and wolfberry polysaccharide are added into a mixer according to the formula ratio and mixed evenly, melatonin and vitamin B6 are added, and mixed at a low speed for 10 minutes to avoid inactivation of functional ingredients, and finally functional probiotic powder is added and mixed evenly under low temperature conditions; S4. Particle size screening: remove uneven particles through an 80-mesh sieve to obtain a finished oyster peptide composition.

4. The method for preparing an oyster peptide composition having the function of improving sleep according to claim 3, characterized in that: The enzymatic hydrolysis temperature is 50-55°C, pH is 7.0, and the time is 6 hours.

5. The method for preparing an oyster peptide composition having the function of improving sleep according to claim 3, characterized in that: The drying equipment is a spray dryer, the air inlet temperature of the spray dryer is 150°C, and the air outlet temperature is 80°C.

6. The method for preparing the oyster peptide composition for improving sleep according to claim 3, characterized in that: The vacuum degree of the vacuum condition is 10 -3 Pa~10 -2 Pa.

7. The method for preparing the oyster peptide composition for improving sleep according to claim 3, characterized in that: The mixing condition parameters of the mixer are: temperature 10-20° C., stirring speed 150-220 rpm, and time 15-20 min.

8. The method for preparing the oyster peptide composition for improving sleep according to claim 3, characterized in that: The low temperature condition is 15-25° C., and the mixing time is 15-20 minutes.

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