Repair composition for full-effect protection and prevention of acne skin and application thereof

Abstract

The invention provides a repairing composition for full-effect protection and prevention of acne skin and application of the repairing composition, and belongs to the technical field of cosmetics. According to the repairing composition, the sulforaphane, the lactoferrin, the bromelain, the chalonic acid lactone and the guaiacol extract are compounded to serve as active ingredients, the five substances can achieve a synergistic effect and act together from multiple aspects, the skin protection force is enhanced, the skin light damage is improved, the oxidative stress level is reduced, the influence of pressure hormone cortisol is reduced, and the repairing composition has a good repairing effect on the skin. The mood enthusiasm is improved, so that the effects of preventing acnes, smoothing the skin and enhancing the barrier function are achieved.

Description

Technical Field

[0001] The present disclosure relates to the technical field of cosmetics, and in particular to a repairing composition for all-round protection and prevention of acne skin and its application. Background Art

[0002] Acne vulgaris is a skin disease that often occurs during adolescence and youth. The main mechanisms of occurrence are hyperkeratosis, obstruction of sebaceous glands, androgen stimulation of sebaceous gland secretion, and colonization of Propionibacterium acnes in the sebaceous glands, causing inflammation. Acne can be divided into whiteheads, blackheads, papules, pustules, and nodules and cysts based on phenotype and severity. However, a person's environment, emotions, living habits, and physiological state are all important factors affecting the occurrence and evolution of acne.

[0003] Exposure of the skin to environmental pollution or UV rays can induce acne. Pollutants exert harmful effects by inducing oxidative stress in the skin through ROS and inflammation. ROS cause a lipid peroxidation cascade, stimulating keratinocytes and melanocytes to release other proinflammatory mediators, thus establishing a vicious inflammatory cycle. UV rays damage skin cells, and on the other hand, primary skin damage caused by acne can cause pain and discomfort, which may have psychological consequences and social stress, which can induce or aggravate acne conditions.

[0004] Acne often attracts people's attention only during the outbreak period. Skin lesions, pigmentation and negative emotions caused by acne are important reasons for the recurrence of acne, so daily protection and conditioning are essential. At present, there is a lack of a repair composition that can protect against environmental pollution, resist UV radiation, improve negative emotions, repair the barrier and prevent acne. Summary of the invention

[0005] The purpose of the present disclosure is to overcome the deficiencies of the prior art and to provide a repair composition and application thereof for all-round protection and prevention of acne skin, wherein the repair composition can protect against environmental pollution, resist UV radiation, improve negative emotions, repair the skin barrier and prevent acne.

[0006] To achieve the above objectives, the technical solution adopted by the present disclosure is as follows: In the first aspect, a repair composition is provided, comprising the following components in parts by weight: 1-10 parts of sulforaphane, 1-10 parts of lactoferrin, 0.1-1 parts of bromelain, 0.1-5 parts of mevalonolactone, and 5-15 parts of guaiac extract.

[0007] In some embodiments, the preparation method of the guaiac extract is: adding guaiac powder to a low eutectic solvent, extracting at a temperature of 50-70°C for 1-2h, separating the solid and the liquid, and the obtained liquid is the guaiac extract, wherein the low eutectic solvent is composed of dipalmitoyl hydroxyproline and dipropylene glycol.

[0008] In some embodiments, the molar ratio of dipalmitoyl hydroxyproline to dipropylene glycol is 1:1-1:3;

[0009] And / or, the volume ratio of the guaiac wood powder to the low eutectic solvent is 1 g:10 mL to 1 g:30 mL.

[0010] In some embodiments, the preparation method of bromelain is: pineapple, cellulase, hemicellulase and PBS buffer solution are mixed evenly, enzymolysis is performed at a temperature of 25-35° C. for 0.5 h, and solid-liquid separation is performed, and the obtained liquid is bromelain.

[0011] In some embodiments, the mass of the cellulase is 0.4-0.8% of the mass of the pineapple, and the mass of the hemicellulase is 0.3-0.5% of the mass of the pineapple;

[0012] And / or, the solid-liquid ratio of the pineapple to the PBS buffer solution is 1 g:1 mL-1 g:1.5 mL.

[0013] In a second aspect, a use of the repair composition in the preparation of cosmetics is provided.

[0014] In a third aspect, a cosmetic is provided, comprising the repairing composition, wherein the content of the repairing composition in the cosmetic is 0.5-3 wt %.

[0015] In some embodiments, the cosmetic is at least one of lotion, emulsion, cream, mask, essence, and spray.

[0016] In some embodiments, the cosmetic is an essence, and the essence includes the following components in percentage by weight: 0.5-3% of a repair composition, 1-20% of a moisturizer, 0.05-0.5% of a thickener, 0.05-0.1% of a pH adjuster, 0.5-10% of an emulsifier, 0.2-3% of a preservative, 0.05-0.5% of a fragrance, and the remainder being water.

[0017] In some embodiments, the moisturizer includes at least one of glycerin, butylene glycol, and low molecular weight sodium hyaluronate;

[0018] And / or, the thickener includes at least one of xanthan gum, Carbopol 980, and high molecular weight cellulose;

[0019] And / or, the emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethyl silsesquioxane;

[0020] And / or, the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone;

[0021] And / or, the pH adjuster includes at least one of arginine and disodium EDTA.

[0022] Compared with the prior art, the beneficial effects of the present disclosure are as follows: the repair composition of the present invention uses sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract as active ingredients, and the five substances can synergistically enhance the effect, work together from multiple aspects, enhance the skin's protective ability, improve skin photodamage, reduce the level of oxidative stress, reduce the influence of the stress hormone cortisol, and enhance emotional positivity, thereby achieving the effects of preventing acne, smoothing the skin, and enhancing barrier function. Among them, sulforaphane, lactoferrin, and guaiac extract cooperate with each other to improve photodamage and reduce stress emotional factors, thereby improving the repair ability of acne-prone skin and enhancing pleasant emotions; mevalonolactone, bromelain, and guaiac extract cooperate with each other to effectively protect against environmental pollution, enhance skin antioxidant capacity, smooth the skin, prevent acne, and strengthen the barrier. The combination of the five active substances can further enhance the above effects and make the skin healthier. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 is the death judgment standard of zebrafish embryos in effect example 1;

[0024] Figure 2 This is a diagram showing the protective effects of the repair compositions of Example 1 and Comparative Example 1 on zebrafish embryos;

[0025] Figure 3 This is a diagram showing the protective effect of the repair compositions of Example 1 and Comparative Example 1 on the tail fin of zebrafish in Effect Example 2;

[0026] Figure 4 This is a diagram showing the improvement effect of the essence in Application Example 1 on facial acne;

[0027] Figure 5 This is a graph showing the improvement effect of the essence in Comparative Application Example 1 on facial acne. DETAILED DESCRIPTION

[0028] In order to facilitate understanding of the present disclosure, the present disclosure will be described more fully below. However, the present disclosure can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present disclosure more thorough and comprehensive.

[0029] As used herein:

[0030] "Prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0031] The conjunction "consisting of excludes any unspecified element, step, or component. If used in a claim, this phrase renders the claim closed-ended so that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the body of a claim rather than immediately following the subject matter, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0032] When amount, concentration or other value or parameter is represented by range, preferred range or a series of upper preferred value and lower preferred value, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1-5", described range should be interpreted as including range "1-4", "1-3", "1-2", "1-2 and 4-5", "1-3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0033] In these examples, parts and percentages are by mass unless otherwise indicated.

[0034] "Parts by mass" refers to the basic unit of measurement for expressing the mass ratio of multiple components. 1 part can represent any unit mass, such as 1g or 2.689g. If we say that the mass of component A is a parts and the mass of component B is b parts, it means the ratio of the mass of component A to the mass of component B is a:b. Alternatively, it means that the mass of component A is aK and the mass of component B is bK (K is an arbitrary number, indicating a multiple factor). It should not be misunderstood that, unlike the mass parts, the sum of the mass of all components is not limited to 100 parts.

[0035] "And / or" is used to indicate that one or both of the stated situations may occur, for example, A and / or B includes (A and B) and (A or B).

[0036] In a first aspect, a repair composition is provided, comprising the following components in parts by weight: 1-10 parts of sulforaphane, 1-10 parts of lactoferrin, 0.1-1 parts of bromelain, 0.1-5 parts of mevalonolactone, and 5-15 parts of guaiac extract.

[0037] For example, in different embodiments, the weight of the sulforaphane can be, but is not limited to, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts; preferably 1.2-6 parts;

[0038] For example, in different embodiments, the weight portion of lactoferrin can be, but is not limited to, 1 part, 1.5 parts, 2.5 parts, 3.5 parts, 4.5 parts, 5.5 parts, 6.5 parts, 7.5 parts, 8.5 parts, 9.5 parts, 10 parts; preferably 1.1-5 parts;

[0039] For example, in different embodiments, the weight portion of the bromelain can be, but is not limited to, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, or 1; preferably 0.3-0.8;

[0040] For example, in different embodiments, the weight portion of the mevalonolactone may be, but is not limited to, 0.1, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5; preferably 0.5-2.5;

[0041] For example, in different embodiments, the weight proportion of the guaiac extract may be, but is not limited to, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 parts; preferably, 6-10 parts.

[0042] The repair composition of the present invention uses sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract as active ingredients. The five substances can synergistically enhance the effect and work together from multiple aspects to enhance the skin's protective ability, improve skin photodamage, reduce the level of oxidative stress, reduce the influence of stress hormone cortisol, and improve emotional positivity, thereby achieving the effects of preventing acne, smoothing the skin, and enhancing barrier function. Among them, sulforaphane, lactoferrin, and guaiac extract cooperate with each other to improve photodamage and reduce stress emotional factors, thereby improving the repair ability of acne-prone skin and enhancing pleasant emotions; mevalonolactone, bromelain, and guaiac extract cooperate with each other to effectively protect against environmental pollution, enhance skin's antioxidant capacity, smooth the skin, prevent acne, and strengthen the barrier. The combination of the five active substances can further enhance the above effects and make the skin healthier.

[0043] In some embodiments, the preparation method of the guaiac extract is: adding guaiac wood powder to a low eutectic solvent, extracting at a temperature of 50-70°C for 1-2h, separating the solid and the liquid, and the obtained liquid is the guaiac extract, wherein the low eutectic solvent is composed of dipalmitoyl hydroxyproline and dipropylene glycol.

[0044] The present invention uses dipalmitoyl hydroxyproline as a hydrogen bond acceptor and dipropylene glycol as a hydrogen bond donor to form a low eutectic solvent for extracting the guaiac extract. The low eutectic solvent can effectively increase the content of active ingredients in the guaiac extract, thereby improving the repair composition's effects of improving photodamage, reducing stress and emotional factors, protecting against environmental pollution, and resisting oxidation and removing acne.

[0045] For example, in different embodiments, the extraction temperature may be, but is not limited to, 50°C, 52°C, 54°C, 56°C, 58°C, 60°C, 62°C, 64°C, 66°C, 68°C, 70°C;

[0046] For example, in different embodiments, the extraction time can be, but is not limited to, 1 h, 1.2 h, 1.5 h, 1.8 h, or 2 h.

[0047] In some embodiments, the molar ratio of dipalmitoylhydroxyproline to dipropylene glycol is 1:1-1:3, for example, but not limited to 1:1, 1:1.2, 1:1.5, 1:1.7, 1:2, 1:2.3, 1:2.5, 1:2.8, 1:3;

[0048] In some embodiments, the volume ratio of the guaiac wood powder to the low eutectic solvent is 1g:10mL-1g:30mL, for example, it can be but not limited to 1g:10mL, 1g:12mL, 1g:15mL, 1g:17mL, 1g:20mL, 1g:25mL, 1g:28mL, 1g:30mL.

[0049] The present disclosure has found that within the above-mentioned parameter range, the content of effective active ingredients in the guaiac extract can be further increased, thereby improving the overall effect of the repair composition.

[0050] Specifically, in order to further improve the effective active ingredient content and extraction efficiency of the guaiac extract, the average particle size of the guaiac wood powder is less than 20 meshes.

[0051] Specifically, there is no limitation on the method of solid-liquid separation, as long as the solid and liquid can be separated, such as centrifugation, filtration, suction filtration, etc. The present invention preferably uses centrifugation as the method of solid-liquid separation, the centrifugal speed is 3000-6000r / min, and the centrifugal time is 10-20min.

[0052] Specifically, the preparation method of the low eutectic solvent is not specifically limited in the present invention. The low eutectic solvent can be prepared by known methods in the art, for example, the hydrogen bond donor and the hydrogen bond acceptor are mixed evenly under heating conditions to obtain a low eutectic solvent, and the heating temperature is 40-60°C.

[0053] In some embodiments, the preparation method of bromelain is: pineapple, cellulase, hemicellulase and buffer solution are evenly mixed, enzymolysis is performed at a temperature of 25-35° C. for 30-60 minutes, and solid-liquid separation is performed, and the obtained liquid is bromelain.

[0054] The present invention discloses extracting bromelain from pineapple peel residue using cellulase and hemicellulase. The obtained bromelain has high activity and can effectively improve the effects of the repair composition in improving photodamage, reducing stress and emotional factors, protecting against environmental pollution, anti-oxidation and removing acne.

[0055] For example, in different embodiments, the extraction temperature may be, but is not limited to, 25°C, 26°C, 27°C, 28°C, 29°C, 30°C, 31°C, 32°C, 33°C, 34°C, 35°C;

[0056] For example, in different embodiments, the extraction time can be, but is not limited to, 30 min, 35 min, 40 min, 45 min, 50 min, 55 min, or 60 min.

[0057] In some embodiments, the mass of the cellulase is 0.4-0.8% of the mass of the pineapple, for example, it can be but not limited to 0.4%, 0.5%, 0.6%, 0.7%, 0.8%;

[0058] The mass of the hemicellulase is 0.3-0.5% of the mass of the pineapple, for example, it can be but not limited to 0.3%, 0.35%, 0.4%, 0.45%, 0.5%;

[0059] In some embodiments, the solid-liquid ratio of the pineapple and PBS buffer solution is 1g:1mL-1g:1.5mL, for example, it can be but not limited to 1g:1mL, 1g:1.1mL, 1g:1.2mL, 1g:1.3mL, 1g:1.4mL, 1g:1.5mL.

[0060] The present disclosure has found that within the above parameter range, the activity of bromelain can be further increased, thereby improving the overall effect of the repair composition.

[0061] In a second aspect, a use of the repair composition in the preparation of cosmetics is provided.

[0062] In a third aspect, a cosmetic is provided, comprising the repairing composition, wherein the content of the repairing composition in the cosmetic is 0.5-3wt%, for example, but not limited to 0.5%, 0.7%, 1%, 1.2%, 1.5%, 1.7%, 2%, 2.3%, 2.5%, 2.8%, 3%.

[0063] In addition to the above-mentioned extracts, the composition used according to the invention generally comprises a physiologically acceptable and preferably cosmetically acceptable medium, meaning a medium suitable for contact with human skin without toxicity, incompatibility, instability or allergic reaction, in particular without causing discomfort (redness, tightness, stinging). Advantageously, the cosmetic can be in the form of a powder, emulsion, microemulsion, nanoemulsion, suspension, solution, lotion, cream, aqueous or hydroalcoholic gel, foam, solution or dispersion for aerosol or lipid vesicle dispersion. In the case of an emulsion, it can be a water-in-oil or oil-in-water emulsion.

[0064] In some embodiments, the cosmetic is at least one of lotion, emulsion, cream, mask, essence, and spray.

[0065] In some embodiments, the cosmetic is an essence, and the essence includes the following components in percentage by weight: a repair composition 0.5-3%, a moisturizer 1-20%, a thickener 0.05-0.5%, a pH adjuster 0.05-0.1%, an emulsifier 0.5-10%, a preservative 0.2-3%, a fragrance 0.05-0.5%, and the remainder water.

[0066] For example, in different embodiments, the mass percentage of the repair composition may be, but is not limited to, 0.5%, 0.7%, 1%, 1.2%, 1.5%, 1.7%, 2%, 2.3%, 2.5%, 2.8%, 3%;

[0067] For example, in different embodiments, the mass percentage of the moisturizing agent may be, but is not limited to, 1%, 3%, 5%, 7%, 9%, 11%, 13%, 15%, 17%, 19%, 20%;

[0068] For example, in different embodiments, the mass percentage of the thickener may be, but is not limited to, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%;

[0069] For example, in different embodiments, the mass percentage of the pH adjuster may be, but is not limited to, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, or 0.1%;

[0070] For example, in different embodiments, the mass percentage of the emulsifier may be, but is not limited to, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%;

[0071] For example, in different embodiments, the mass percentage of the preservative may be, but is not limited to, 0.2%, 0.4%, 0.6%, 0.8%, 1%, 1.5%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%;

[0072] For example, in different embodiments, the mass percentage of the flavor may be, but is not limited to, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, or 0.5%.

[0073] In some embodiments, the moisturizer includes at least one of glycerin, butylene glycol, and low molecular weight sodium hyaluronate;

[0074] In some embodiments, the thickener includes at least one of xanthan gum, Carbopol 980, and high molecular weight cellulose;

[0075] In some embodiments, the emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane;

[0076] In some embodiments, the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone;

[0077] In some embodiments, the pH adjuster includes at least one of arginine and disodium EDTA.

[0078] The cosmetics of the present invention can be prepared by any suitable method known in the art. For example, the cosmetics can be prepared using equipment such as a dissolving tank, an emulsifying pot, a disperser, and a delivery pump commonly used in the cosmetics field according to processes known in the art. For example, a water-soluble substance can be first put into an aqueous phase dissolving pot, and an oil-soluble substance (such as a sunscreen, grease) can be put into an oil phase dissolving pot, and the temperature of the two pots can be heated to about 80°C, respectively. For raw materials that are easy to agglomerate, they can be pre-dispersed with a disperser; such as powder-containing substances (such as zinc oxide and titanium dioxide), they can be dispersed with a suitable solvent and a dispersant and homogenized for 5-10 minutes, and the oil phase is added and homogenized again for 5-10 minutes; after homogenization is completed, the oil phase and the water phase are delivered to the emulsifying pot for homogenization and emulsification for about 5-15 minutes; after emulsification is completed, the temperature of the material body is reduced to room temperature, and optionally, flavors, preservatives, etc. are added, and the pH of the product is adjusted as needed; after the relevant test indicators are qualified, the material can be filled and discharged.

[0079] The above preparation process is merely exemplary, and those skilled in the art can increase, decrease or adjust it according to the dosage form requirements to prepare various dosage forms such as spray, emulsion, gel / jelly, cream, cushion / foundation, etc.

[0080] In order to further illustrate the present invention, the repair composition and application thereof provided by the present invention are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0081] The raw materials used in the embodiments and comparative examples are described below, but are not limited to these materials:

[0082] The guaiac extract 1 was homemade, and its preparation method was as follows: adding guaiac wood powder with an average particle size of less than 20 mesh into a low eutectic solvent, extracting at a temperature of 60°C for 1.5 hours, centrifuging the obtained product at a speed of 6000r / min for 15 minutes, and the obtained supernatant was the guaiac extract, which was stored at a temperature of 4°C for later use; wherein, the low eutectic solvent was composed of dipalmitoylhydroxyproline and dipropylene glycol in a molar ratio of 1:2, and the mass ratio of the guaiac wood powder to the volume ratio of the low eutectic solvent was 1g:20mL.

[0083] Guaiac extract 2 was homemade, and its preparation method was different from that of guaiac extract 1 only in that the extraction temperature was 50 °C, the extraction time was 2 h, the mass of guaiac wood powder and the volume ratio of the low eutectic solvent was 1 g:10 mL, and the remaining steps and parameters were exactly the same.

[0084] Guaiac extract 3 was homemade, and its preparation method was different from that of guaiac extract 1 only in that the extraction temperature was 70°C, the extraction time was 1 h, the mass of guaiac wood powder and the volume ratio of the low eutectic solvent was 1 g:30 mL, and the remaining steps and parameters were exactly the same.

[0085] Guaiac extract 4 was prepared in-house, and its preparation method was different from that of guaiac extract 1 only in that the low eutectic solvent consisted of dipalmitoyl hydroxyproline and glycerol in a molar ratio of 1:2.

[0086] Guaiac extract 5 was homemade, and its preparation method was different from that of guaiac extract 1 only in that the low eutectic solvent consisted of betaine and dipropylene glycol in a molar ratio of 1:2.

[0087] Guaiac Extract 6 was prepared in-house, and its preparation method was different from that of Guaiac Extract 1 only in that 60% by volume ethanol was used to replace the low eutectic solvent.

[0088] Guaiac extract 7 was prepared in-house, and its preparation method was different from that of Guaiac extract 1 only in that water was used to replace the low eutectic solvent.

[0089] Bromelain 1 is homemade, and its preparation method is as follows: 100 parts by weight of pineapple peel residue, 0.6 part by weight of cellulase, 0.4 part by weight of hemicellulase and PBS buffer solution of pH=7 are mixed evenly, stirred for enzymolysis at a temperature of 30°C for 30min, filtered, the obtained filtrate is centrifuged at a rotating speed of 6000r / min for 15min, 1 volume of 90% ethanol is added to the obtained supernatant, and after continuing to centrifuge under the same conditions, the obtained supernatant is bromelain, which is stored at a temperature of-20°C for standby use, and the solid-liquid ratio of pineapple peel residue to PBS buffer solution is 1g:1.2mL.

[0090] Bromelain 2 was homemade, and its preparation method was different from that of bromelain 1 only in that the solid-liquid ratio of pineapple peel residue to PBS buffer solution was 1 g:1 mL, and the remaining steps and parameters were exactly the same.

[0091] Bromelain 3 was homemade, and its preparation method was different from that of bromelain 1 only in that the solid-liquid ratio of pineapple peel residue to PBS buffer solution was 1 g:1.5 mL, and the remaining steps and parameters were exactly the same.

[0092] Bromelain 4 is homemade, and its preparation method is as follows: 20 parts by weight of pineapple peel residue and 60 parts by weight of PBS buffer solution with pH=7 are mixed evenly, filtered with 4 layers of gauze, and the filtrate is placed in a refrigerated centrifuge, centrifuged at 3000rpm / min at 4°C for 6min, and the obtained supernatant is bromelain, which is stored at a temperature of -20°C for use.

[0093] Sulforaphane: product number S111997, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.;

[0094] Glucoraphanin: Product number B20804, purchased from Shanghai Yuanye Biotechnology Co., Ltd.;

[0095] Lactoferrin: Product number B1576395, purchased from Bachem;

[0096] Lactoferrin: Product number L890179, purchased from Shanghai MacLean Biochemical Technology Co., Ltd.;

[0097] Mevalonolactone: Product number Y29876, purchased from Shanghai Yuanye Biotechnology Co., Ltd.;

[0098] Gluconolactone: Product No. A22918 purchased from Shandong West Asia Chemical Co., Ltd.;

[0099] Licorice extract: Product number S69134, purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0100] Examples and Comparative Examples

[0101] The present invention provides a repair composition according to the embodiment and comparative example. The components of the repair composition are shown in Table 1 and Table 2.

[0102] The preparation method of the repair composition of the embodiment and comparative example is:

[0103] Weigh each component according to the weight parts in Table 1 and Table 2;

[0104] adding sulforaphane and mevalonolactone to glycerol, wherein the mass ratio of the total mass of sulforaphane and mevalonolactone to the mass of glycerol is 1:2, and uniformly stirring by ultrasonication to obtain a first mixture;

[0105] adding lactoferrin, bromelain and guaiac extract into deionized water, wherein the mass ratio of the total mass of lactoferrin, bromelain and guaiac extract to the deionized water is 1:1, and uniformly stirring with ultrasonic stirring to obtain a second mixture;

[0106] The first mixture and the second mixture are mixed and ultrasonically stirred to obtain a repair composition.

[0107] Table 1

[0108]

[0109]

[0110] Table 2

[0111]

[0112] Application examples 1-15, comparative application examples 1-10 and blank application examples

[0113] The application examples, comparative application examples and blank application examples of the present invention provide an essence, the components (mass percentage) of the essence are shown in Table 3; wherein the repair compositions used in application examples 1-14 are respectively the repair compositions prepared in embodiments 1-14, the repair compositions used in comparative application examples 1-10 are respectively the repair compositions prepared in comparative examples 1-10; the repair composition used in application example 15 is the repair composition prepared in embodiment 1;

[0114] The preparation methods of the essences of the application examples, comparative application examples and blank application examples are as follows:

[0115] (1) Component A and water are mixed and stirred, heated to 80° C., homogenized at a speed of 1200 rpm for 5 minutes, and kept warm for later use to obtain prefabricated component A;

[0116] (2) Mixing component B, heating to 80° C., homogenizing at 1200 rpm for 5 min, and keeping warm for later use to obtain prefabricated component B;

[0117] (3) mixing the C components and heating them to 60° C. to melt them to obtain a prefabricated C component;

[0118] (4) Heat the preformed component A to 80°C, add the preformed component B at a speed of 300 rpm, stir and mix, then cool to 60°C, add the preformed component C at a speed of 300 rpm, stir and mix, then cool to below 45°C, add the repair composition and continue stirring for 5 minutes, finally add arginine to adjust the pH to 5.0-6.5, then stop stirring, discharge, and obtain the essence.

[0119] Table 3

[0120]

[0121] Effect Example 1

[0122] This effect example tests the protective effect of the repair composition of the embodiment and the comparative example on environmental pollutants.

[0123] Environmental pollution has a variety of toxic effects on human skin, damaging the skin barrier and inducing acne. This effect example verifies the protective effect of the repair composition proposed in the present invention on environmental pollutants based on the model organism zebrafish. Zebrafish belongs to the genus Brachydanio, the subclass of Actinopterygii, the family of Cyprinidae, and has strong reproductive capacity, a short reproductive cycle, and is very sensitive to the stimulation of toxic substances. It has a highly conserved genome and signal transduction pathway, which is highly homologous to human genes. It has been widely used in skin-related research fields. The specific test steps are:

[0124] Zebrafish species: Use wild-type AB strain zebrafish (Danio rerio) from a reliable source (such as the China National Zebrafish Resource Center) for spawning tests.

[0125] Zebrafish embryo preparation: Under 26°C, fertilized fish embryos complete the first division in about 45 minutes, and then divide into 4, 8, 16 and 32 cells. At this time, the fertilized fish embryos are clearly identifiable. Select healthy fish embryos in the 4-32 cell stage for use, and the exposure to the test substance should start in the 4-128 cell stage.

[0126] Preparation of test substances: dimethyl sulfoxide was used to adjust the concentration of each repair composition to 0.1%, which was recorded as the sample solution. 0.4 mg / L tetrabromobisphenol A was selected as the positive control group.

[0127] Group setting: Sample group, set up 10 parallel wells, add 1 zebrafish embryo and 0.2mL sample solution to each well; Model group, set up 10 parallel wells, add 1 zebrafish embryo and 0.2mL tetrabromobisphenol A + sample solution (1:1) to each well; Blank control group, set up 10 parallel wells, add 1 zebrafish embryo and 0.2mL fish embryo culture medium to each well, the embryo culture medium is prepared as follows: weigh 2940mg anhydrous calcium chloride, 1233mg magnesium sulfate heptahydrate, 630mg sodium bicarbonate, 55mg potassium chloride and dissolve in 10L water, pH 7.5. Fish embryo culture medium, at least 90% of the tested fish embryos survived at the end of the test, otherwise it needs to be re-prepared. Each group was placed in a 26℃ incubator for 72 hours. After the exposure, each zebrafish embryo was observed under a microscope and the number of dead embryos in each group was recorded. The survival rate was calculated as 1-(number of surviving embryos before the test-number of surviving embryos after the test) / number of surviving embryos before the test × 100%. The criteria for determining the death of fish embryos were: coagulation, absence of heartbeat, or detachment of the tail, as shown in the following table. Figure 1 The test results are shown in Table 4 and Figure 2 shown.

[0128] Table 4

[0129]

[0130]

[0131] Note: When the t-test method is used for statistical analysis, the significance of the model group compared with the blank control group is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; the significance of the sample group compared with the blank control group is indicated by *, P-value < 0.05 is indicated by *, P-value < 0.01 is indicated by **, and P-value < 0.001 is indicated by ***.

[0133] It can be seen from the experimental data in Table 4 that the survival rate of zebrafish embryos is over 40% when the repair composition of the present invention is used for protection, indicating that the repair composition of the present invention has a good protective effect on environmental pollutants.

[0134] From Examples 1-5, it can be seen that when the repair composition includes the following components in parts by weight: 1.2-6 parts of sulforaphane, 1.1-5 parts of lactoferrin, 0.3-0.8 parts of bromelain, 0.5-2.5 parts of mevalonolactone, and 6-10 parts of guaiac extract, the survival rate of zebrafish embryos is more than 50% when the repair composition of the present invention is used for protection, indicating that the repair composition within the above range has a better protective effect against environmental pollutants.

[0135] By comparing Example 1 and Examples 6-11, it can be seen that the guaiac extract is extracted using a low eutectic solvent composed of dipalmitoyl hydroxyproline and dipropylene glycol to prepare a repair composition, and the repair composition is used to protect against environmental pollutants. The survival rate of zebrafish embryos reaches more than 55%, indicating that the obtained guaiac extract can effectively improve the protective effect of the repair composition against environmental pollutants.

[0136] By comparing Example 1 with Examples 12-14, it can be seen that the use of cellulase, hemicellulase and bromelain extracted with PBS buffer can greatly improve the protective effect of the repair composition against environmental pollutants.

[0137] By comparing Example 1 with Comparative Examples 1-5, it can be seen that without any one of sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract, the protective effect of the obtained repair composition against environmental pollutants is significantly reduced.

[0138] By comparing Example 1 with Comparative Examples 6-10, it can be seen that when any one of sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract is replaced with other substances with similar effects, the protective effect of the obtained repair composition against environmental pollutants is significantly reduced.

[0139] Effect Example 2

[0140] This effect example tests the skin protection effect of the repair composition of the embodiment and the comparative example against the damage caused by the stress hormone cortisol.

[0141] When stress increases, the sebaceous glands secrete excessive oil, inducing inflammation and worsening acne. Cortisol is a known stress hormone. When cortisol levels rise, it damages skin health. This effect effectively verifies the skin protective effect of the repair composition provided by the present invention against the stress hormone cortisol. In the process of wound repair, zebrafish do not clot blood, and the rest of the steps are the same as humans. It can be used as a test model for the efficacy of human skin repair. This effect example uses the stress hormone cortisol to delay the healing process of zebrafish skin, establishes a cortisol skin damage model, and verifies the skin protective effect of the repair composition provided by the present invention against the stress hormone cortisol. The specific experimental steps are:

[0142] (1) The zebrafish species and embryo preparation were consistent with those in Example 1;

[0143] (2) Preparation of test substances: dimethyl sulfoxide was used to adjust the concentration of each repair composition to 5 mg / L, which was recorded as the sample solution; cortisol solution was prepared by weighing cortisol powder and dissolving it in water to prepare a 5 mg / L storage solution, and the working solution was diluted 100 times with fish embryo culture medium; tricaine solution was prepared by weighing 200 mg of tricaine and dissolving it in 48.95 mL of water and refrigerating;

[0144] (3) Fin removal step: anesthetize the zebrafish embryo with tricaine solution and remove the caudal fin of the zebrafish embryo with a laboratory scalpel under a microscope;

[0145] (4) Group setting: The blank control group was set as 24 fish embryos randomly selected, the fins were removed and transferred to a 96-well plate, each well containing one fish embryo and 0.2 mL of fish embryo culture medium; the model control group was set as 24 fish embryos randomly selected, the fins were removed and transferred to a 96-well plate, each well containing one fish embryo and 0.2 mL of cortisol solution; the sample group was set as 24 fish embryos randomly selected, the fins were removed and transferred to a 96-well plate, each well containing one fish embryo and 0.2 mL of the test substance solution + cortisol solution; and the cells were placed in a constant temperature of 28°C and cultured for 3 h;

[0146] (5) Sample microscopic analysis: The zebrafish was anesthetized with tricaine and then placed under a stereomicroscope to take side-on photos of the tail of the fish embryo, and the length changes were analyzed pixel by pixel.

[0147] (6) Data and result calculation: Count the length of the caudal fin of each fish embryo on the 7th day (pixels). Calculate the promotion rate of caudal fin repair: Promotion rate = (BA) / (CA)×100%,

[0148] Among them, A: the average tail fin length of fish embryos in the blank control group; B: the average tail fin length of fish embryos in the model control group; C: the average tail fin length of fish embryos in the sample group.

[0149] The test results are shown in Table 5 and Figure 3 shown.

[0150] Table 5

[0151] Group Promotion rate (%) Example 1 139.31 Example 2 134.64 Example 3 127.18 Example 4 125.85 Example 5 125.52 Example 6 138.90 Example 7 137.71 Example 8 124.55 Example 9 124.87 Example 10 123.59 Embodiment 11 123.96 Example 12 138.51 Embodiment 13 138.11 Embodiment 14 135.01 Comparative Example 1 109.09 Comparative Example 2 105.26 Comparative Example 3 104.71 Comparative Example 4 103.63 Comparative Example 5 111.73 Comparative Example 6 110.70 Comparative Example 7 111.32 Comparative Example 8 110.91 Comparative Example 9 108.70 Comparative Example 10 107.91

[0152] It can be seen from the experimental data in Table 5 that the zebrafish tail fin repair promotion rate reaches more than 123% when the repair composition of the present invention is used, indicating that the repair composition of the present invention has a good skin protection effect against the stress hormone cortisol.

[0153] From Examples 1-5, it can be seen that when the repair composition includes the following components in parts by weight: 1.2-6 parts of sulforaphane, 1.1-5 parts of lactoferrin, 0.3-0.8 parts of bromelain, 0.5-2.5 parts of mevalonolactone, and 6-10 parts of guaiac extract, the zebrafish tail fin repair promotion rate reaches more than 130% when the repair composition of the present invention is used, indicating that the repair composition within the above range has a better skin protection effect against the stress hormone cortisol.

[0154] By comparing Example 1 and Examples 6-11, it can be seen that the guaiac extract is extracted using a low eutectic solvent composed of dipalmitoyl hydroxyproline and dipropylene glycol to prepare a repair composition. Using the repair composition, the zebrafish tail fin repair promotion rate reaches more than 135%, indicating that the obtained guaiac extract can effectively improve the skin protective effect of the repair composition against the stress hormone cortisol.

[0155] By comparing Example 1 with Examples 12-14, it can be seen that the repair composition is prepared using bromelain extracted with cellulase, hemicellulase and PBS buffer. Using the repair composition, the zebrafish tail fin repair promotion rate reaches more than 138%, indicating that the bromelain can greatly improve the skin protective effect of the repair composition against the stress hormone cortisol.

[0156] Comparative Example 1 and Comparative Examples 1-5 show that the lack of any one of sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract significantly reduces the skin protective effect of the obtained repair composition against the stress hormone cortisol.

[0157] By comparing Example 1 with Comparative Examples 6-10, it can be seen that when any one of sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract is replaced with other substances with similar efficacy, the skin protective effect of the obtained repair composition against the stress hormone cortisol is significantly reduced.

[0158] Effect Example 3

[0159] This effect example tests the skin protection effect of the repairing composition of the embodiment and the comparative example against UVB radiation.

[0160] When the skin is exposed to ultraviolet rays, it will damage skin cells, cause skin damage, create an inflammatory environment, and promote the occurrence of acne. Therefore, this effect example establishes a UVB damaged skin cell model to explore the repair effect of the repair composition provided by the present invention on light-damaged skin cells. The specific steps are as follows:

[0161] Take a bottle of human skin fibroblasts (purchased from Kunming Cell Bank, Chinese Academy of Sciences) in good exponential growth phase, add 0.25% trypsin digestion solution, digest to make the adherent cells fall off, and count 1-4×10 5 / mL, make a cell suspension, and inoculate it in a 96-well plate. Aspirate the complete medium in the well, add PBS, and expose it to UVB light for 15 minutes (irradiation intensity: 80μW / cm 2); the blank control group was added with complete culture medium without irradiation. After irradiation, the PBS solution was aspirated, the experimental group was added with complete culture medium, and the sample was added to a final concentration of 3.12%; the model group was added with PBS. Each group was cultured for 24 hours. MTT reagent was added to a 96-well plate and reacted in an incubator for 4 hours. The supernatant was aspirated, DMSO was added, and the plate was shaken on a plate shaker for 5 minutes. Subsequently, the reference OD values ​​at wavelengths of 490nm and 630nm were read using an ELISA reader, and the cell viability was calculated. Cell viability = (OD of the dosing well - OD of the zeroing well) / (OD of the control well - OD of the zeroing well) × 100%. The test results are shown in Table 6.

[0162] Table 6

[0163] Group Cell viability (%) Blank control group 100 Model Group 58.23# Example 1 72.34* Example 2 71.18* Example 3 71.35* Example 4 70.26* Example 5 70.34* Example 6 72.10* Example 7 72.32* Example 8 70.24* Example 9 70.13* Example 10 70.09* Embodiment 11 70.03* Example 12 72.13* Embodiment 13 72.22* Embodiment 14 71.06* Comparative Example 1 59.20 Comparative Example 2 56.24 Comparative Example 3 45.18 Comparative Example 4 38.23 Comparative Example 5 46.17 Comparative Example 6 40.22 Comparative Example 7 49.18 Comparative Example 8 35.20 Comparative Example 9 54.15 Comparative Example 10 50.21

[0164] Note: When the t-test method is used for statistical analysis, the significance of the model group compared with the blank control group is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; the significance of the sample group compared with the blank control group is indicated by *, P-value < 0.05 is indicated by *, P-value < 0.01 is indicated by **, and P-value < 0.001 is indicated by ***.

[0166] It can be seen from the experimental data in Table 6 that, using the repair composition of the present invention, the cell viability of human skin fibroblasts reaches more than 70%, indicating that the repair composition of the present invention has a good skin protection effect against UVB radiation.

[0167] From Examples 1-5, it can be seen that when the repair composition includes the following components in parts by weight: 1.2-6 parts of sulforaphane, 1.1-5 parts of lactoferrin, 0.3-0.8 parts of bromelain, 0.5-2.5 parts of mevalonolactone, and 6-10 parts of guaiac extract, the cell viability of human skin fibroblasts reaches more than 71% when the repair composition of the present invention is used, indicating that the repair composition within the above range has a better skin protection effect against UVB radiation.

[0168] By comparing Example 1 and Examples 6-11, it can be seen that the guaiac extract is extracted using a low eutectic solvent composed of dipalmitoyl hydroxyproline and dipropylene glycol to prepare a repair composition. Using the repair composition, the cell viability of human skin fibroblasts reaches more than 72%, indicating that the obtained guaiac extract can effectively improve the skin protection effect of the repair composition against UVB radiation.

[0169] By comparing Example 1 with Examples 12-14, it can be seen that the repair composition is prepared using bromelain extracted with cellulase, hemicellulase and PBS buffer. Using the repair composition, the cell viability of human skin fibroblasts reaches more than 72%, indicating that the bromelain can greatly improve the skin protection effect of the repair composition against UVB radiation.

[0170] By comparing Example 1 with Comparative Examples 1-5, it can be seen that in the absence of any one of sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract, the skin protective effect of the obtained repair composition against the stress hormone cortisol is significantly reduced, indicating that sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract work synergistically to improve the skin protective effect of the repair composition against UVB radiation.

[0171] By comparing Example 1 with Comparative Examples 6-10, it can be seen that when any one of sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract is replaced with other substances with similar efficacy, the skin protection effect of the obtained repair composition against UVB radiation is significantly reduced.

[0172] Effect Example 4

[0173] This effect example tests the human efficacy of the application example, the comparative application example and the blank application example.

[0174] The specific test method is as follows:

[0175] According to the Technical Specifications for Safety of Cosmetics (2015), 200 subjects with sensitive skin aged 18-60 years (lactic acid stinging score > 3) were selected for the test. Under normal circumstances, the subjects underwent a random, half-face test, with 8 people in each group, and used the product continuously for 28 days. Instrumental testing and clinical evaluation of skin indicators were performed on the 0th, 7th, 14th, 28th days of the test and on the 7th day after discontinuation of the product.

[0176] 1. The specific steps of instrument measurement are as follows:

[0177] Every morning and evening, after cleansing and moisturizing, the subjects applied 1 mL of the product lotion to one side of the face and massaged for absorption. The other side of the face used a blank application example lotion (without a repair composition), and used it once in the morning and once in the evening. On the day of the visit, the subjects washed their faces with clean water without applying any products. They sat quietly in an air-conditioned room with a temperature of 21±1°C and a humidity of 50±10% for 20 minutes. Instruments were used to quantify and photograph the skin condition, including Tewameter TM Hex to measure TEWL; acne counts (papules), which were evaluated by doctors; VC 20Plus to measure the degree of roughness; data were recorded and the index improvement rate was calculated, index improvement rate = (value before use - value after use) / value before use × 100%. The results are shown in Tables 7 and Figure 4-5As shown, T0 represents the period before using the product, T1 represents the 28th day of the test during the use of the product, and T2 represents the 7th day of the test during the suspension of the product.

[0178] Table 7

[0179]

[0180]

[0181] It can be seen from the experimental data in Table 7 that on the 28th day of using the cream of the present invention, the improvement rate of TEWL was 9.25-11.29%, the improvement rate of acne count was 36.51-41.25%, and the improvement rate of roughness was 9.34-16.27%; on the 7th day of stopping the use of the cream of the present invention, the improvement rate of TEWL was 1.18-3.34%, the improvement rate of acne count was 20.23-28.46%, and the improvement rate of roughness was 5.34-11.44%; it can be seen that the cream containing the repairing composition of the present invention has good moisturizing, skin smoothing and acne prevention effects.

[0182] From Examples 1-5, it can be seen that when the repair composition includes the following components in parts by weight: 1.2-6 parts of sulforaphane, 1.1-5 parts of lactoferrin, 0.3-0.8 parts of bromelain, 0.5-2.5 parts of mevalonolactone, and 6-10 parts of guaiac extract, the improvement rate of TEWL is 10.18-11.29%, the improvement rate of acne count is 38.44-41.25%, and the improvement rate of roughness is 12.44-16.27% on the 28th day of using the cream containing the repair composition; on the 7th day of stopping the use of the cream containing the repair composition, the improvement rate of TEWL is 2.4-3.31%, the improvement rate of acne count is 26.76-28.46%, and the improvement rate of roughness is 9.88-11.44%; it shows that the cream containing the repair composition has better moisturizing, skin smoothing and acne prevention effects.

[0183] By comparing Example 1 with Examples 6-11, it can be seen that the repair composition is prepared by extracting the guaiac extract using a low eutectic solvent composed of dipalmitoyl hydroxyproline and dipropylene glycol. On the 28th day of using the cream containing the repair composition, the improvement rate of TEWL is 11.25-11.29%, the improvement rate of acne count is 41.14-41.25%, and the improvement rate of roughness is 15.68-16.27%; on the 7th day after stopping the use of the cream containing the repair composition, the improvement rate of TEWL is 3.3-3.34%, the improvement rate of acne count is 28.3-28.46%, and the improvement rate of roughness is 11.21-11.44%; it can be seen that the cream containing the improved repair composition has better moisturizing, skin smoothing and acne prevention effects.

[0184] By comparing Example 1 with Examples 12-14, it can be seen that the repair composition is prepared using cellulase, hemicellulase and bromelain extracted with PBS buffer. On the 28th day of using the cream containing the repair composition, the improvement rate of TEWL is 11.25-11.29%, the improvement rate of acne count is 41.18-41.25%, and the improvement rate of roughness is 15.9-16.27%; on the 7th day of stopping the use of the cream containing the repair composition, the improvement rate of TEWL is 3.31-3.33%, the improvement rate of acne count is 28.34-28.46%, and the improvement rate of roughness is 11.13-11.44%; it shows that the cream containing the improved repair composition has better moisturizing, skin smoothing and acne prevention effects.

[0185] By comparing Example 1 with Comparative Examples 1-5, it can be seen that in the absence of any one of sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract, the improvement rate of TEWL was less than 6%, the improvement rate of acne count was less than 18%, and the improvement rate of roughness was less than 5% on the 28th day of using the cream containing the repair composition; on the 7th day of stopping the use of the cream containing the repair composition, the improvement rate of TEWL was less than 0%, the improvement rate of acne count was less than 9%, and the improvement rate of roughness was less than 3%; it indicates that sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract work synergistically to improve the moisturizing, skin smoothing and acne prevention effects of the cream.

[0186] By comparing Example 1 with Comparative Examples 6-10, it can be seen that when any one of sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract is replaced by other substances with similar efficacy, the improvement rate of TEWL is less than 4.5%, the improvement rate of acne count is less than 17%, and the improvement rate of roughness is less than 5.5% on the 28th day of using the cream containing the repair composition; on the 7th day of stopping the use of the cream containing the repair composition, the improvement rate of TEWL is less than 0%, the improvement rate of acne count is less than 6%, and the improvement rate of roughness is less than 2%; it shows that only when sulforaphane, lactoferrin, bromelain, mevalonolactone and guaiac extract work together, can they have excellent moisturizing, skin smoothing and acne prevention effects.

[0187] Finally, it should be noted that the above embodiments are used to illustrate the technical solution of the present disclosure rather than to limit the protection scope of the present disclosure. Although the present disclosure has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present disclosure can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present disclosure.

Claims

1. A repair composition, characterized in that: The invention comprises the following components in parts by weight: 1-10 parts of sulforaphane, 1-10 parts of lactoferrin, 0.1-1 parts of bromelain, 0.1-5 parts of mevalonolactone and 5-15 parts of guaiac wood extract.

2. The repair composition according to claim 1, characterized in that The preparation method of the guaiac wood extract comprises: adding guaiac wood powder into a low eutectic solvent, extracting at a temperature of 50-70° C. for 1-2 hours, and separating the solid from the liquid, and obtaining the liquid, which is the guaiac wood extract, wherein the low eutectic solvent is composed of dipalmitoyl hydroxyproline and dipropylene glycol.

3. The repair composition according to claim 2, characterized in that The molar ratio of dipalmitoyl hydroxyproline to dipropylene glycol is 1:1-1:3; And / or, the volume ratio of the guaiac wood powder to the low eutectic solvent is 1 g:10 mL-1 g:30 mL.

4. The repair composition according to claim 1, characterized in that The preparation method of bromelain comprises the following steps: evenly mixing pineapple, cellulase, hemicellulase and PBS buffer, performing enzymolysis at a temperature of 25-35° C. for 0.5 h, and performing solid-liquid separation to obtain bromelain in the form of liquid.

5. The repair composition according to claim 4, characterized in that The mass of the cellulase is 0.4-0.8% of the mass of the pineapple, and the mass of the hemicellulase is 0.3-0.5% of the mass of the pineapple; And / or, the solid-liquid ratio of the pineapple to the PBS buffer solution is 1 g:1 mL-1 g:1.5 mL.

6. Use of the repair composition according to any one of claims 1 to 5 in the preparation of cosmetics.

7. A cosmetic, characterized in that: The cosmetic comprises the repairing composition according to any one of claims 1 to 5, and the content of the repairing composition in the cosmetic is 0.5 to 3 wt%.

8. The cosmetic according to claim 8, characterized in that The cosmetic is at least one of lotion, emulsion, cream, mask, essence and spray.

9. The cosmetic according to claim 8, characterized in that The cosmetic is an essence, and the essence comprises the following components in percentage by mass: 0.5-3% of a repairing composition, 1-20% of a moisturizing agent, 0.05-0.5% of a thickener, 0.05-0.1% of a pH regulator, 0.5-10% of an emulsifier, 0.2-3% of a preservative, 0.05-0.5% of a fragrance, and the remainder being water.

10. The cosmetic according to claim 9, characterized in that The moisturizing agent includes at least one of glycerin, butylene glycol, and low-molecular sodium hyaluronate; And / or, the thickener includes at least one of xanthan gum, Carbopol 980, and high molecular weight cellulose; And / or, the emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethyl silsesquioxane; And / or, the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone; And / or, the pH adjuster includes at least one of arginine and disodium EDTA.

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