Application and method of intensified sensitizer in algae-polluted water body

Abstract

The invention belongs to the field of polluted water treatment, and particularly relates to application of an enhanced sensitizer in algae polluted water and a specific application method. The invention relates to an application of a fortified substance in an algae-polluted water body, which is characterized in that the fortified substance is at least one of salicylic acid, p-hydroxybenzoic acid, pyrogallic acid, gallic acid, catechol, linoleic acid, L-lysine and malonic acid; the algae refer to microcystis algal blooms. The sensitizing substance adopted in the application is a natural plant extract, and the raw material resources are rich in source, non-toxic and high in safety; the enhanced sensitizer has obvious algae removal and inhibition effects, and has good environmental benefits; for example, for a microcystis aeruginosa polluted water body, the highest inhibition rate can reach 99.52% on the 19th day of a test.

Description

Technical Field

[0001] The invention belongs to the field of polluted water treatment, and specifically relates to the application of an enhanced sensitizing substance in algae polluted water, and a specific application method. Background Art

[0002] Blue algae blooms have become a major hidden danger affecting the safety of drinking water sources, and harmful algae blooms have become a global problem that needs to be solved urgently in the field of water environment. Studies have shown that the frequency of algae blooms is increasing worldwide, the scale of impact is expanding, and the duration is getting longer and longer. Because 25% to 70% of the world's blue algae blooms can produce algae toxins, water bodies polluted by blue algae blooms will release a variety of different types of algae toxins into the water body after the toxic blue algae cells rupture, causing more serious secondary disasters and ecological safety risks. Therefore, it is of great significance to control the occurrence of blue algae blooms, reduce drinking water source pollution and reduce ecological safety risks.

[0003] At present, the commonly used emergency control methods for cyanobacterial blooms include physical algae removal and chemical algae removal. Among them, chemical algae removal is widely used because of its wide application, quick effect and good effect. Chemical algaecides include quicklime, copper sulfate, modified alum, modified clay, 2,2-dibromo-3-nitropropionamide (DBNPA), etc. Although these methods are useful, they have obvious limitations, including difficult operation, high cost, secondary pollution and invasive species. Therefore, it is particularly urgent to find an efficient, economical and environmentally friendly method to control cyanobacterial blooms. The discovery of allelopathic substances provides a new idea for the control of cyanobacterial blooms. Allelopathic algae control is considered to be a very potential method for bloom control due to its eco-friendly characteristics, especially in the prevention and control of cyanobacteria in high-functional water bodies such as drinking water sources. The chemical structure of allelopathic substances can be divided into 14 categories, such as water-soluble organic acids, long-chain fatty acids and polyacetylenes, simple phenols, terpenes and steroids, benzoic acid and its derivatives, cinnamic acid and its derivatives, simple unsaturated lactones, benzoquinones, anthraquinones and complex quinones, coumarins, flavonoids, tannins, amino acids and peptides, alkaloids and cyanohydrins, sulfides and glucosinolates, purines and nucleosides. So far, scientists have discovered allelopathic substances that can effectively inhibit the growth of algae from more than 40 large aquatic plants. There are three main characteristics of the action of allelopathic substances, namely, specific or specific inhibition or promotion, "low promotion and high inhibition", and "combined effects of addition, synergy, antagonism or independent action". Among them, some allelopathic substances have stronger allelopathic effects on algae and have greater application potential. This patent intends to propose a new algae removal method and application that can quickly remove algae and allelopathically overcome algae using enhanced allelopathic substances as materials.

[0004] At present, there are many kinds of allelopathic substances that can be used for algae removal, but some allelopathic substances have the problem of unsatisfactory algae removal effect, or they have a certain inhibitory effect on algae production in a short time after algae removal, but as time goes by, algae begin to grow again, that is, the inhibitory effect of enhanced allelopathic substances on Microcystis is reversible and does not have the ability to continuously control algae. In addition, different algae have different characteristics and will show different algae inhibition effects for different enhanced allelopathic substances. This also makes enhanced allelopathic substances a key factor in whether algae inhibition is successful in algae removal. It has been disclosed in the literature that the use of allelopathic substances such as gallic acid or pyrogallic acid to treat Microcystis has a significant effect. However, for small water bodies or large water bodies in the wild, since the algae are not controlled during the growth process, the algae are affected by photosynthesis, and are affected by the direction of water waves and wind direction, making the treatment of wild water bodies complicated, and the laboratory treatment method is not suitable for algae treatment in the wild.

[0005] Therefore, it is necessary to target the above-mentioned problems, screen for enhancing substances that can effectively remove Microcystis blooms, and invent effective methods for small water bodies or large water bodies in the wild. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides a potentiating substance suitable for treating cyanobacteria blooms, especially Microcystis blooms, and provides a specific method for using the potentiating substance in treating Microcystis blooms. Combined with the specific potentiating substance and the treatment method, the inhibition rate of Microcystis is high and the sustained algae inhibition ability is strong. In particular, the present invention also provides a method for treating algae in outdoor flowing water bodies.

[0007] The present invention focuses on the use of an intensifying substance in algae-polluted water, wherein the intensifying substance refers to at least one of pyrogallol, gallic acid, catechol, linoleic acid, and L-lysine.

[0008] Algae refers to blue algae blooms. The enhanced algae-killing substance in the present invention has no special requirements for the water body with harmful Microcystis blooms, and is particularly suitable for water bodies polluted by Microcystis aeruginosa.

[0009] When the enhanced material is solid, a solid powder with a particle size of 200-400 mesh is used; when it is liquid, the liquid density is maintained at 0.890-0.920×10 3 kg / m 3 The present invention adopts solid powder with a particle size of 200-400 mesh or liquid density of 0.890-0.920×10 3 kg / m 3The allelopathic substances of algae have a large specific surface area, and some allelopathic substances such as phenolic acids, ketones, and terpenes are strong electron acceptors, which block the electron transport chain by seizing electrons in the PSⅡ electron transport chain. Therefore, allelopathic substances can inhibit the electron transfer rate of the algae photosynthetic system by destroying chlorophyll a in algae cells, affecting the synthesis of adenosine triphosphate (ATP), reducing its photosynthetic efficiency, and further affecting the release of energy, thereby inhibiting the respiration of algae cells and even inhibiting the growth and reproduction of algae.

[0010] Specifically, the method of applying the enhanced susceptibility substance to treat algae-polluted water is as follows: the enhanced susceptibility substance is added to the algae-polluted water, and the mass volume ratio of the enhanced susceptibility substance to the algae-polluted water is (5-80) mg:1L.

[0011] When the temperature of algae-polluted water is between 10 and 40 °C, the enhanced allelopathic substances are added to the algae-polluted water for a treatment time of 1 to 19 days. If it is a small water body, after being added to the algae-polluted water body, the surface water is stirred and mixed at 2 to 5 revolutions per second and 3 to 8 seconds per time for a total of 3 to 5 times. For large water bodies, it is necessary to divert and centrally isolate the floating algae blooms on the water surface, and directly add enhanced allelopathic substances in the diversion and isolation area. After being thrown, the surface water is fan-shaped stirred and mixed by pumping natural water bodies. The scouring intensity is based on the maximum flow rate of 7 to 50 m 3 / h, the maximum shooting distance is set between 6 and 17m.

[0012] In the present invention, large water bodies generally refer to lakes and reservoirs with an area of ​​more than 10 mu and still river surfaces in this field;

[0013] Small water bodies refer to small water surfaces, such as ponds, still water ditches or still river bays with a water surface area of ​​less than 10 mu.

[0014] The material used for the diversion device is a gauze of not less than 200 meshes, and the gauze material can be selected from nylon 6, nylon 66, silk cloth, polyester, carbon fiber or stainless steel. The flexible gauze needs to be laid in 2 to 3 layers, and counterweights and floats are set at the same time. The counterweight requirement is not less than 1.5kg / m net, and the specifications of the non-absorbent float are not less than 5cm wide × 3cm thick × 80cm / m net, and are wrapped with gauze. The overall diversion net is 2 to 3m wide, and the length is determined according to the size of the water surface. The diversion net is mostly laid in a trumpet shape and is set according to the direction of the water flow and the wind direction for the convenience of diversion and collection.

[0015] The beneficial effects of the present invention are:

[0016] (1) The enhanced sensitizing substances used in this application are natural plant extracts, which have abundant raw material resources, are non-toxic, and have high safety;

[0017] (2) The enhanced inhibitory effect of the substances on algae is obvious and has good environmental benefits. For example, for water bodies contaminated by Microcystis aeruginosa, the highest inhibition rate can reach 99.52% on the 19th day of the experiment.

[0018] (3) The method of removing algae with enhanced sensitive substances is simple, just add and mix well. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Attached Figure 1 The inhibitory effects and inhibition rates of the eight allelochemicals in Example 1 on Microcystis aeruginosa;

[0020] Attached Figure 2 To determine the minimum effective algae inhibition concentration of pyrogallic acid;

[0021] Attached Figure 3 To determine the minimum effective algae inhibition concentration of catechol;

[0022] Attached Figure 4 To determine the minimum effective algae inhibition concentration of linoleic acid;

[0023] Attached Figure 5 The cell density of Microcystis aeruginosa changes with time under the treatment of different mass concentrations of gallic acid;

[0024] Attached Figure 6 is the inhibition rate of the cell density growth of Microcystis aeruginosa under the treatment of different mass concentrations of gallic acid;

[0025] Attached Figure 7 The chlorophyll a content of Microcystis aeruginosa cells changes with time under the treatment of different mass concentrations of gallic acid;

[0026] Attached Figure 8 The inhibition rate of chlorophyll a content in Microcystis aeruginosa cells under different concentrations of gallic acid treatment;

[0027] Attached Fig. 9 is a schematic diagram of the structure of the diversion device;

[0028] Attached Fig.10 Schematic diagram of the arrangement of diversion devices for water treatment;

[0029] Fig. 9 , 10 In the figure, 1-floating strip, 2-guiding net, 3-counterweight, 201-section A guiding net, 202-section B guiding net, 203-section C guiding net, 204-closing point, 4-shore. DETAILED DESCRIPTION

[0030] In order to enable those skilled in the art to better understand the present invention, the present invention is further described in conjunction with specific implementation methods.

[0031] In this application, the raw materials used are as follows:

[0032] Salicylic acid: purity 98%, AR, produced by Shanghai McLean Biochemical Technology Co., Ltd.;

[0033] Para-hydroxybenzoic acid: purity 98%, AR, produced by Shanghai McLean Biochemical Technology Co., Ltd.;

[0034] Pyrogallol: purity 98%, AR, produced by Shanghai McLean Biochemical Technology Co., Ltd.;

[0035] Gallic acid: purity 98%, AR, produced by Shanghai McLean Biochemical Technology Co., Ltd.;

[0036] Catechol: purity 98%, AR, produced by Shanghai McLean Biochemical Technology Co., Ltd.; Linoleic acid: liquid, purity 95%, AR, produced by Shanghai McLean Biochemical Technology Co., Ltd.;

[0037] L-lysine: purity 98%, AR, produced by Shanghai McLean Biochemical Technology Co., Ltd.; Malonic acid: purity 98%, AR, produced by Shanghai McLean Biochemical Technology Co., Ltd.

[0038] Example 1

[0039] The treatment effects of different enhanced sensitizers on water bodies polluted by Microcystis aeruginosa were investigated. The specific steps are as follows:

[0040] (1) Eight allelochemicals, including salicylic acid, p-hydroxybenzoic acid, pyrogallic acid, gallic acid, catechol, linoleic acid, L-lysine, and malonic acid, were selected as the substances that are considered to have strong inhibitory effects on algal growth;

[0041] (2) Take the liquid of Microcystis aeruginosa grown to the logarithmic phase (initial algae density: 2.12×10 7 cells / mL), and exposed them to the above eight allelopathic substances (the dosage of allelopathic substances was 60mg / L per liter of algae liquid polluted water), and placed them in an intelligent light incubator for culture. The culture conditions were: temperature 25±1℃, light intensity 3000lx. The trend of algae cell density over culture time was measured and compared with the control group to determine the influence of various allelopathic substances on the growth of algae.

[0042] (3) Treatment method for algae-contaminated water: After addition, shake directly, shake 3 times / second, 5 seconds / time, mix 3 times, and let stand for reaction; the inhibitory effects and inhibition rates of the eight allelopathic substances on Microcystis aeruginosa after different addition times are investigated, as shown in Tables 1 and 2 and attached Figure 1 Directly adding enhanced allelopathic substances into the diversion isolation area and the materials used in the diversion measures are as described in the invention content.

[0043] The control group was a group without any enhancement substances added, and only the above-mentioned (3) was performed.

[0044] The algae cell density detection equipment and method are as follows:

[0045] The algae density was measured by hemocytometer (25 × 16) (inverted microscope, Olympus, Japan).

[0046] Table 1 Inhibitory effects of eight allelochemical treatments on the cell density of Microcystis aeruginosa

[0047]

[0048]

[0049] Table 2 Inhibition rate of eight allelochemical treatments on the cell density of Microcystis aeruginosa

[0050]

[0051] Figure 1 The changes over time of the algal cell density and growth inhibition rate of Microcystis aeruginosa exposed to the above eight allelopathic substances.

[0052] Combined with Table 1 and Appendix Figure 1 ,from Figure 1 It can be clearly seen in Figure a that in the nearly 20-day experiment of eight allelopathic substances inhibiting algae, only pyrogallic acid, catechol and linoleic acid effectively controlled the growth of the cell density of Microcystis aeruginosa. At the end of the experiment (the 19th day), the inhibition rates of the three substances on algae reached the highest level, which were 99.52%, 92.77% and 98.19% respectively. Figure 1 b. It is worth noting that gallic acid and L-lysine showed significant algae inhibition effects in the first 5 days and 15 days of the experiment, respectively, with the highest inhibition rates reaching 89.49% and 77.80%, respectively. Figure 1 b), however, the inhibitory effect of the two on algae is reversible, and the algae cell density began to rise after 5 and 15 days respectively, so it is believed that they do not have the ability to continuously control algae. Among them, salicylic acid, p-hydroxybenzoic acid and malonic acid have the weakest inhibitory effect on algae. Although these three have a certain inhibitory effect on algae after 1 day of the test, throughout the entire test period, the growth trend of algae cell density exposed to these two allelopathic substances is basically consistent with that of the control group.

[0053] Based on the results in Example 1, it is preliminarily determined that the three allelopathic substances, pyrogallic acid, catechol and linoleic acid, have a high algae inhibition rate and have the ability to continuously inhibit algae.

[0054] Gallic acid and L-lysine have obvious algae inhibition ability in the early stage, but their sustained algae control ability is not as good as pyrogallic acid, catechol and linoleic acid.

[0055] Example 2

[0056] The minimum effective algae inhibition concentrations of the three enhanced algae substances in Example 1 were determined as follows:

[0057] On the basis of Example 1, three allelopathic substances with relatively excellent effects were screened out. A test was designed to determine the minimum effective algae inhibition concentration of the three allelopathic substances (respectively recorded as pyrogallic acid group, catechol group and linoleic acid group). The allelopathic substance addition concentration was 60 mg / L, and the allelopathic substance addition concentration range was determined to be 0-80 mg / L. Microcystis aeruginosa grown to the logarithmic phase (algae density ≈4.86×10 6 cells / mL) were exposed to the above three allelopathic substances with concentration gradients of 0 (ck), 5, 10, 20, 40, 60 and 80 mg / L respectively and cultured in a light incubator. The algal cell density was measured and the growth curve of the algal cell density over time was drawn.

[0058] Table 3 Changes in algal cell density in the pyrogallic acid treatment group at different culture times

[0059]

[0060] Table 4 Changes in algal cell density in the catechol treatment group at different culture times

[0061]

[0062] Table 5 Changes in algal cell density in the linoleic acid treatment group at different culture times

[0063]

[0064]

[0065] Combined with the above table and attached Figure 2-4 , Figure 2-4 The cell density of Microcystis aeruginosa exposed to three allelopathic substances of different mass concentrations changes over time. As can be seen from the figure, the cell density of the control group increased rapidly over time, from 4.87×10 6 cells / mL increased to 1.054×10 7cells / mL, in contrast, the algae cells in the treatment groups treated with the three allelopathic substances were inhibited to varying degrees and showed a concentration-dependent trend. Among them, in the treatment group with pyrogallic acid, the algae cell density of the 5mg / L treatment group continued to decline for two days and then began to recover rapidly, the algae cell density of the 10mg / L treatment group continued to decline until the fifth day and then roughly stabilized, and the algae cell density of the 20mg / L treatment group began to show a continuous downward trend; in the treatment group with catechol, the algae cell density of the 5mg / L treatment group continued to decline for two days and then began to slowly recover, and the algae cell density of the 10mg / L treatment group began to show a continuous downward trend; and in the treatment group with linoleic acid, only 5mg / L linoleic acid can cause the algae cell density to continue to decline. Since the algae cell density began to maintain a continuous downward trend at the addition dosages of 20 mg / L, 10 mg / L and 5 mg / L of pyrogallic acid, catechol and linoleic acid respectively, through calculation, the algae cell density in the 20 mg / L pyrogallic acid, 10 mg / L catechol and 5 mg / L linoleic acid treatment groups only decreased by 61.3%, 64.5% and 66.5% respectively at the end of the experiment. Therefore, it is believed that the minimum effective inhibitory concentration ranges of the three allelopathic substances of pyrogallic acid, catechol and linoleic acid on algae are between 20-40 mg / L, 10-20 mg / L and 5-10 mg / L respectively.

[0066] Example 3

[0067] The inhibitory effect of different concentrations of enhanced sensitizers on algae was investigated. Although in Example 1, pyrogallic acid was used to treat water bodies polluted by Microcystis aeruginosa, it is not suitable for large-scale application due to its higher cost and unknown ecological risks. Therefore, taking into account the cost, algae inhibition effect, natural and wide sources, gallic acid was taken as an example in Example 3 to investigate its influence on the change trend of the cell density of Microcystis aeruginosa over time and the inhibition rate of the growth of the cell density of Microcystis aeruginosa, as follows:

[0068] Gallic acid was prepared into five concentration gradients (5, 10, 20, 40 and 80 mg / L), and their algal cell concentrations and inhibition rates on Microcystis aeruginosa were investigated on days 0, 1, 2, 5, 7 and 10, respectively.

[0069] Table 6 Dynamic effects of different concentrations of gallic acid on the cell density of Microcystis aeruginosa at different culture times

[0070]

[0071]

[0072] Table 7 Dynamic changes in the inhibition rate of different mass concentrations of gallic acid on the cell density growth of Microcystis aeruginosa at different culture times

[0073]

[0074] Figure 5 and Figure 6 They are the change trend of the cell density of Microcystis aeruginosa exposed to different mass concentrations of gallic acid over time and the inhibition rate of the growth of the cell density of Microcystis aeruginosa at five concentration gradients (5, 10, 20, 40 and 80 mg / L) of gallic acid over time. During the 10-day test, the cell density of the 5 and 10 mg / L gallic acid treatment groups was close to that of the control group (CK), while the cell density of the 20-80 mg / L treatment group was significantly lower than that of the control group (P < 0.05), showing an overall trend of "high concentration significantly inhibited, low concentration had almost no effect" ( Figure 5 ). In the initial stage of the experiment (1st to 3rd day), the algal cell density in the 5 and 10 mg / L treatment groups was slightly higher than that in the control group, showing a certain promotion effect, while the algal cell density in the 20-80 mg / L treatment group was significantly lower than that in the control group as a whole (P < 0.05), especially in the 80 mg / L treatment group on the first day of the experiment, its algal cell density decreased by 18.2% compared with that before treatment. From the 4th day of the experiment, the algal cell density in the 5 and 10 mg / L treatment groups was slightly lower than that in the control group (P > 0.05), showing a certain inhibitory effect. During the experiment, the inhibition rate of algal cell density in the 20-80 mg / L treatment group continued to increase, with the highest inhibition rates reaching 32.53%, 49.39% and 74.69% respectively ( Figure 6 ). After 10 days of treatment, the EC of gallic acid on the inhibition of algal cell density 50 The value is 37.7mg / L.

[0075] Example 4

[0076] The effects of different concentrations of gallic acid on the chlorophyll a content of Microcystis aeruginosa in the exponential growth phase were studied. Gallic acid was prepared into five concentration gradients (5, 10, 20, 40 and 80 mg / L). The algal cell concentrations at 0, 1, 2, 5, 7 and 10 days, the changes in the chlorophyll a content of Microcystis aeruginosa over time, and the inhibition rate of the growth of chlorophyll a content were investigated.

[0077] Table 8 Dynamic effects of different concentrations of gallic acid on the chlorophyll a content of Microcystis aeruginosa at different culture times

[0078]

[0079]

[0080] Table 9 Dynamic changes in the inhibition rate of different mass concentrations of gallic acid on chlorophyll a of Microcystis aeruginosa at different culture times

[0081]

[0082] Figure 7 and Figure 8 They are respectively the temporal change trend of chlorophyll a content in Microcystis aeruginosa exposed to different mass concentrations of gallic acid and the temporal change of the inhibition rate of gallic acid at five concentration gradients (5, 10, 20, 40 and 80 mg / L) on the growth of chlorophyll a content in Microcystis aeruginosa.

[0083] The chlorophyll a content of the control group was basically consistent with the growth trend of algae cell density during the whole experiment, while the growth of chlorophyll a content of all treatment groups was affected to varying degrees and showed a concentration-dependent trend. On the first day of the experiment, the chlorophyll a content of the control group increased normally, the chlorophyll a content of the 5 mg / L treatment group increased slowly but not significantly compared with the control group (P>0.05), the chlorophyll a content of the 10 mg / L treatment group almost did not increase, the chlorophyll a content of the 20-40 mg / L treatment group decreased compared with the control group, but not significantly (P>0.05), and the chlorophyll a content of the 80 mg / L treatment group decreased significantly compared with the control group (P<0.05), and decreased by 6.6%, 22.3% and 64.8% respectively compared with the previous day. After the first day of the experiment, the chlorophyll a content of all treatment groups began to increase continuously. The growth trend of chlorophyll a content in the treatment group with a concentration lower than 40 mg / L was basically consistent with the algal cell density, and gradually became slow with the increase of gallic acid exposure concentration within this concentration range, while the chlorophyll a content in the treatment group with a concentration higher than (including) 40 mg / L showed a linear growth trend during the experiment without any accelerated growth trend. On the 10th day of the experiment, according to the experimental results, it was calculated that the EC value of gallic acid's inhibitory effect on chlorophyll a of Microcystis aeruginosa was 50 The value is 42.6mg / L.

[0084] Example 5

[0085] Although the use of enhanced sensitive substances to treat Microcystis aeruginosa in the laboratory simulation of algae-contaminated water has a significant effect, it will be affected by various factors when treating water in small water bodies or large water surfaces. Unlike the laboratory simulation of algae wastewater, the water waves in the wild water body are flowing, and the algae will float with the water flow, making it difficult to gather and treat them centrally; and, under the photosynthesis of algae, the pH in the water will continue to change, and the dissolved oxygen will also increase, which also increases the difficulty of algae treatment. That is, the method with excellent treatment effect in the laboratory is not necessarily suitable for algae treatment in the wild, and it must be improved in a targeted manner according to the growth of algae in the wild. The inventor discovered this problem when treating algae-contaminated water in the wild.

[0086] Based on the growth characteristics of algae, the inventors found that algae often grow 1-1.5 meters below the water surface and are in a floating state. Based on this characteristic, the inventors specifically used a diversion device to concentrate the algae in one area, and then injected corresponding allelopathic substances into the area, combined with physical methods such as collecting algae, to effectively control the growth of algae in the treatment area, so that the water in the area does not affect the drinking water supply of millions of people.

[0087] Location of treated water body: Typical area of ​​algal bloom in the main stream of Xinjiang River, Yingtan City, Jiangxi Province

[0088] Treatment plan: Investigate the effect of diversion devices on the treatment of cyanobacteria blooms;

[0089] The specific processing methods are as follows:

[0090] (1) Laying out diversion devices

[0091] A diversion device is installed, and its structure is as follows: the diversion device includes a rectangular diversion net made of two layers of flexible gauze or stainless steel mesh, and the height of the diversion net is 1.5 to 3 meters; a floating strip 1 made of foam is arranged at the upper end of the diversion net; the floating strip 1 is wrapped by the upper end of the diversion net 2 (or called "gauze"), and its width is 4 to 6 cm and its thickness is 2 to 4 cm; 80 cm long floating strips 1 are arranged on each meter of the diversion net 2, and the spacing between adjacent floating strips 1 is equal; the function of the floating strip 1 is to make the upper end of the diversion net 2 just in line with the horizontal plane, and collect the floating algae into the area surrounded by the diversion net 2 for centralized treatment; and the floating strip 1 also has a certain blocking effect on the algae, preventing the algae from floating outside the diversion area under the action of water waves or wind;

[0092] The counterweight 3 is arranged at the lower end of the guide net 2; the counterweight 3 is annular and hung at the lower end of the gauze at equal intervals, and the total weight of the counterweight 3 on each meter of the guide net 2 is 1.5-1.6 kg. Of course, the counterweight 3 can also be other structures, such as a metal strip with holes at both ends, hung at the lower end of the guide net 2. All structures that can play a counterweight role are applicable to the present invention.

[0093] The guide net 2 is a flexible gauze, which can be a net made of nylon 6, nylon 66, silk cloth, polyester, carbon fiber and other materials. The nylon 66 net was used in this experiment; the aperture of the net is 200 mesh; of course, the above-mentioned two layers of gauze can also be three layers of gauze, and such a change also falls within the scope of protection of the present invention; in view of cost considerations and effect inspections, two layers of gauze are actually used in this algae treatment.

[0094] When installing the diversion device, the diversion net 2 is divided into a section A diversion net 201, a section B diversion net 202, and a section C diversion net 203, wherein the section A diversion net 201 is laid out facing the wind direction, and the section A diversion net 201 is substantially parallel to the direction of the water flow;

[0095] The A-section guide net 201 and the C-section guide net 203 roughly form a closed trumpet shape (referred to as the guide area), the A-section guide net 201 is close to the bank 4, and the C-section guide net 203 is far away from the bank 4; the starting end of the A-section guide net 201 is fixed on the bank 4, and the length of the A-section guide net 201 is greater than that of the C-section guide net 203; the trumpet-shaped area surrounded by the A-section guide net 201 and the C-section guide net 203 allows the water flow to flow into the closed part formed by the A-section guide net 201 and the C-section guide net 203, and at the closed part is the B-section guide net 202 which diverges outwards in the form of a fan-shaped strong arc surface; the A-section guide net 201 and the B-section guide net 202 Steel bars are provided at the bends formed at the closing 204 and at the bends formed at the closing 204 of the B-section guide net 202 and the C-section guide net 203, respectively. The steel bars are tied to the guide net, the upper part of the steel bars is roughly flush with the upper end of the guide net, and the lower part is inserted into the mud below the water body at a depth of 1.5 to 2 meters, so that the A-section guide net 201, the B-section guide net 202 and the C-section guide net 203 are collectively surrounded by a trumpet shape and a closing cloth bag shape; the B-section guide net 202 is facing the water flow direction, and the algae bloom is just enriched in the addition area formed by the B-section guide net 202 and the closing 204 under the action of the water flow and wind direction;

[0096] (2) After the diversion device is arranged, mechanical salvage is carried out;

[0097] This time, the water body being treated is a large surface area. Therefore, the algae blooms floating on the water surface will be diverted and isolated, concentrated in the diversion area, and directly pumped out in the diversion area or pumped in from natural water bodies to perform fan-shaped scouring on the surface algae slurry to enhance diversion and collection.

[0098] Before the water treatment, the algae in the area were mainly Microcystis, such as Microcystis aeruginosa, Microcystis aquatica, Microcystis whitleyi or Microcystis planifolia, with an algae density of 7.63×10 7 cells / L. Before the treatment, the wild growth of algae and the sudden increase of organic matter in the water seriously affected the water quality. Therefore, this area was selected as the experimental area for diversion and algae control.

[0099] The specific processing effects are shown in the following table.

[0100] Table 10 Growth of algae before and after treatment

[0101]

[0102]

[0103] It can be seen from Table 10 that the algae blooms were treated with the diversion device in the present application. After 32 days of treatment, the algae inhibition rate of the algae blooms can reach about 75.65%, and the algae cell density has increased from the initial 7.63×10 7 cells / L decreased to 1.86×10 7 cells / L. This shows that the application of the diversion device has played a significant role in inhibiting algae.

Claims

1. The application of the enhanced sensitive substance in algae-polluted water bodies is characterized in that: The enhanced sensory substance is selected from at least one of pyrogallic acid, gallic acid, catechol, linoleic acid, and L-lysine; The algae described refers to cyanobacterial blooms.

2. The use according to claim 1, characterized in that The algae is a bloom of Microcystis aeruginosa.

3. The use according to claim 1, characterized in that When the enhanced sensation substance is solid, a solid powder with a particle size of 200-400 mesh is used; when the enhanced sensation substance is liquid, the liquid density is 0.890-0.920×10 3 kg / m 3 .

4. A method for treating algae-polluted water by using an enhanced sensitizing substance, characterized in that: Take the enhancing substance and add it into the algae-contaminated water body. The mass volume ratio of the enhancing substance to the algae-contaminated water body is (5~80) mg:1L.

5. The method according to claim 4, characterized in that When the enhanced sensitizing substance is pyrogallol, the minimum effective inhibitory concentration range for Microcystis aeruginosa is 20-40 mg / L; When the enhanced sensitizing substance is catechol, the minimum effective inhibitory concentration range for Microcystis aeruginosa is 10-20 mg / L; When the enhancing substance is linoleic acid, the minimum effective inhibitory concentration range for Microcystis aeruginosa is 5-10 mg / L; When the enhancing substance is gallic acid, the EC of inhibition of Microcystis aeruginosa 50 The value is 37.7 mg / L.

6. The method according to claim 4, characterized in that When the temperature of the algae-polluted water body is between 10 and 40°C, the enhanced susceptibility substance is added to the algae-polluted water body, and the treatment time for the algae-polluted water body is 1 to 19 days.

7. The method according to claim 4, characterized in that For small water bodies, after the sensitive substance is added to the algae-contaminated water body, the surface water is stirred and mixed 3 to 5 times at a stirring rate and duration of 2 to 5 revolutions per second and 3 to 8 seconds per time; For large water bodies, after the enhanced sensitive substances are directly added, the natural water body is pumped at a maximum flow rate of 7~50 m 3 / h, and the scouring intensity with the maximum distance of 6~17m stirs the surface water in a fan shape.

8. A method for applying a sensitive substance to treat algae-contaminated water, comprising the following steps: (1) Prepare the enhanced sensation substance. When the enhanced sensation substance is fixed, crush it into a solid powder of 200-400 mesh; when it is liquid, keep its density at 0.890-0.920×10 3 kg / m 3 ; (2) Detect the water temperature of the algae-contaminated water body in the water area to be treated. When the temperature of the algae-contaminated water body is between 10 and 40°C, add it to the algae-contaminated water body to be treated.

9. The method for treating algae-polluted water using a sensitive substance as claimed in claim 8, characterized in that: When the allelopathic agent is added to the algae-polluted water body, a diversion device is used in combination. The diversion device has the following structure: the diversion device comprises a rectangular diversion net made of at least two layers of flexible gauze or stainless steel net, and the height of the diversion net is 1.5 to 3 meters; a floating strip made of multiple sections of foam is arranged at the upper end of the diversion net; and a plurality of counterweights are arranged at the lower end of the diversion net; When installing the diversion device, the diversion net is divided into section A diversion net, section B diversion net, and section C diversion net. The section A diversion net is laid out facing the wind direction, and the section A diversion net is roughly parallel to the direction of the water flow. The A-section guide net and the C-section guide net are roughly formed into a closed trumpet shape, the A-section guide net is close to the shore, and the C-section guide net is far away from the shore; the starting end of the A-section guide net is fixed on the shore, and the length of the A-section guide net is greater than that of the C-section guide net; the trumpet-shaped area surrounded by the A-section guide net and the C-section guide net allows the water flow to flow into the closed part formed by the A-section guide net and the C-section guide net, and the closed part is the B-section guide net that diverges outward with a fan-shaped strong arc surface; the bends formed at the closed part by the A-section guide net and the B-section guide net and the bends formed at the closed part by the B-section guide net and the C-section guide net are respectively provided with steel bars, the upper part of the steel bars is roughly flush with the upper end of the guide net, and the lower part is inserted into the mud below the water body at a depth of 1.5 to 2 meters, so as to make the A-section guide net, the B-section guide net and the C-section guide net jointly form a trumpet shape and a closed cloth bag shape; The diversion net of section B faces the direction of water flow. Under the influence of water flow and wind direction, algae blooms are concentrated in the dosing area formed by the diversion net of section B and the closing part. After arranging the diversion device, add the enhancing substance into the dosing area so that its mass volume ratio to the algae-contaminated water is (5~80) mg:1L.

10. The method for treating algae-polluted water using a sensitive substance as claimed in claim 9, wherein: The floating strips are wrapped by the upper end of the sand net, and have a width of 4-6 cm and a thickness of 2-4 cm. 80 cm long floating strips are arranged on each meter of the gauze net, and the spacing between adjacent floating strips is equal. The counterweight is ring-shaped and hung at equal intervals at the bottom of the gauze. The total weight of the counterweights on each meter of gauze is 1.5~1.6kg.

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