Method for retarding lymphoma cell proliferation cycle based on specific magnetic field parameters

Abstract

A method for slowing down a lymphoma cell proliferation cycle based on specific magnetic field parameters utilizes a low-frequency pulsed magnetic field to open a cell calcium ion pathway so as to promote intracellular calcium homeostasis to achieve the purpose of promoting various physiological processes of cells, and different types of cells have different requirements on calcium ion content in calcium ion channels in the cells and in and out of the cells. Under the same magnetic field parameters, for cells, the promotion of calcium ion internal flow has two effects, namely, the partial physiological process is improved, and some physiological activities can be inhibited if the internal flow is too serious; a special low-frequency pulsed magnetic field is applied to a diffuse large B-cell lymphoma cell line, blank control and drug combined inhibition control are adopted, and the inhibition effect of low-frequency pulsed magnetic field intervention on the cell proliferation cycle of the diffuse large B-cell lymphoma cell line is judged by comparing the cell cycle of the diffuse large B-cell lymphoma cell line.

Description

Technical Field

[0001] The invention belongs to the technical field of cell proliferation, and in particular relates to a method for slowing down the proliferation cycle of lymphoma cells based on specific magnetic field parameters. Background Art

[0002] The existing technology proposes that low-frequency pulsed magnetic fields with special waveforms, frequencies, pulse times, etc. can be used to regulate the cell proliferation rate and proliferation cycle, such as the invention patent applied for by this team in the early stage: the patent is a method based on low-frequency pulsed magnetic fields to induce TRPC1 promotion and enhancement. This patent applies a weak pulsed magnetic field with a special frequency and waiting time to skeletal muscle tissue, activates the TRPC1 transient receptor potential ion channel, promotes the influx of calcium ions in muscle cells and mitochondrial respiration, and promotes the growth and differentiation of muscle cells.

[0003] In the above patent, skeletal muscle cells are placed in a magnetic field with fixed parameters for intervention. CCK8 reagent is used to quantify the cell proliferation rate under magnetic field intervention 24 hours after the intervention. The result is then compared with the blank control group. It is found that magnetic field intervention can accelerate the proliferation rate of skeletal muscle cells. Summary of the invention

[0004] The technical solution adopted by the present invention is: the method for slowing down the proliferation cycle of lymphoma cells based on specific magnetic field parameters uses a low-frequency pulse magnetic field to open the cell calcium ion channel, thereby promoting intracellular calcium homeostasis to achieve the purpose of promoting various physiological processes of the cell. Different types of cells have different requirements for internal calcium ion channels and intracellular and extracellular calcium ion content. That is, under the same magnetic field parameters, for cells, the promotion of calcium ion influx has a two-sided effect, namely, improving some physiological processes, while excessive influx will inhibit certain physiological activities.

[0005] By applying a special low-frequency pulsed magnetic field to the diffuse large B-cell lymphoma cell line, and using a blank control and a drug combined inhibition control, the inhibitory effect of low-frequency pulsed magnetic field intervention on its cell proliferation cycle was determined by comparing the cell cycle of the diffuse large B-cell lymphoma cell line.

[0006] Beneficial effects of the present invention:

[0007] 1. The present invention uses a low-frequency pulsed magnetic field to intervene in diffuse large B-cell lymphoma cell lines to explore the inhibitory effect of the magnetic field on the proliferation cycle of tumor cells, which can be used clinically to treat related diseases.

[0008] 2. The tumor cell cycle is inhibited, and combined with relevant inhibitory drugs, it can greatly slow down the downstream of tumor cells, reducing the pressure on clinical treatment.

[0009] 3. Limiting magnetic field safety: The article Magnetostimulation Limits in Magnetic Particle Imaging proposes that PNS (peripheral nerve stimulation) and SAR (tissue heating) can be effectively avoided under magnetic fields with an intensity below 6mT and a frequency below 25KHz. As a result, the human body will not experience any irritation under magnetic fields within this intensity and frequency range, and related diseases can be treated with low-frequency pulsed magnetic fields in an absolutely safe manner. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 It is a diagram of the cell state after the intervention of the present invention. DETAILED DESCRIPTION

[0011] This experiment uses breast cancer cell lines MCF-7, MDA-MB-231; diffuse large B-cell lymphoma cell line OCI-LY3. This patent verifies the inhibitory effect of low-frequency magnetic field on the proliferation cycle of diffuse large B-cell lymphoma cell lines through two experiments, namely 1) flow cytometry detection of cell cycle 2) cell cloning experiment

[0012] 1. Flow cytometry cell cycle detection experiment: This experiment sets the following groups:

[0013] (1) Blank control group

[0014] (2) Magnetic field intervention group

[0015] (3) CDK4 / 6i positive control group (10nM)

[0016] (4) Magnetic field / inhibitor combined experimental group

[0017] Since the preparation of CDK4 / 6i requires DMSO reagent, in order to eliminate the interference of DMSO on the experimental results, equal amounts of DMSO reagent (10 nM) were added to the blank control group and the magnetic field intervention group.

[0018] The irradiation group was irradiated once every 24 hours for 3 days. After irradiation, the proliferation cycle was analyzed by detecting the chromosome numbers in the G1 phase, S phase and G2 phase of each group of cells to obtain the following data.

[0019] Group Inhibition rate Blank control group 0％ Magnetic field intervention group 25.2％ CDK4 / 6i positive control group (10nM) 43.7％ Magnetic field / inhibitor combined experimental group 51.3％

[0020] 2. Cell cloning experiment: The experimental grouping is the same as the above flow cytometry cell cycle experiment, namely

[0021] (1) Blank control group

[0022] (2) Magnetic field intervention group

[0023] (3) CDK4 / 6i positive control group (10nM)

[0024] (4) Magnetic field / inhibitor combined experimental group

[0025] Before the experiment, the cells in each group were photographed and counted. The reagents and magnetic field intervention scheme used in the experiment were consistent with the above experiment. After the magnetic field intervention lasted for 3 days, crystal violet staining was used to take photos and count the cells after the intervention. Figure 1 .

[0026]

[0027]

[0028] According to the above experimental results, magnetic intervention has a certain inhibitory effect on the proliferation cycle and cloning cycle of the diffuse large B-cell lymphoma cell line OCI-LY3, and whether from the perspective of cloning or proliferation cycle, the best effect is achieved by combining inhibitors and low-frequency pulsed magnetic fields.

[0029] The method proposed in this patent has a positive promoting effect on the treatment of related tumor diseases. This technology can be applied to clinical treatment for the inhibition of the cycle of diffuse large B-cell lymphoma cell line OCI-LY3. However, the shortcoming is that the inhibition experiment is only carried out through a single magnetic field parameter. In the later stage, the proliferation inhibition effect on the diffuse large B-cell lymphoma cell line under different parameters can be explored by changing the relevant magnetic field parameters.

Claims

1. A method for slowing down the proliferation cycle of lymphoma cells based on specific magnetic field parameters, characterized in that: Low-frequency pulsed magnetic fields are used to open cellular calcium ion pathways, thereby promoting intracellular calcium homeostasis to achieve the purpose of promoting various cellular physiological processes. Different types of cells have different requirements for internal calcium ion channels and calcium ion content inside and outside the cells. That is, under the same magnetic field parameters, for cells, the promotion of calcium ion influx has a two-sided effect, namely, improving some physiological processes, while excessive influx will inhibit certain physiological activities.

2. The method for slowing down the proliferation cycle of lymphoma cells based on specific magnetic field parameters according to claim 1, characterized in that: By applying a special low-frequency pulsed magnetic field to the diffuse large B-cell lymphoma cell line, and using a blank control and a drug combined inhibition control, the inhibitory effect of low-frequency pulsed magnetic field intervention on its cell proliferation cycle was determined by comparing the cell cycle of the diffuse large B-cell lymphoma cell line.

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