Detector and detection method for Hensi small body

Abstract

The detector comprises an incubation system and a detection system, a test tube moving mechanism is arranged at the rear end of a test tube rack storage area in a sliding mode, a sample transferring mechanism is arranged at the front end of the test tube rack storage area, and a sample incubation mechanism and a sample adding mechanism are arranged on the right side of an incubation box. The sample adding mechanism is arranged on the sample incubation mechanism; the AI intelligent analysis system is electrically connected to the image acquisition system, a lens of the image acquisition system faces the objective table, the objective table is arranged in the detection box in a front-back and left-right movable mode through the motion system control unit, the light source control unit is arranged below the objective table, and a light source can penetrate through the objective table to irradiate a slide. And a slide transfer head of the slide moving mechanism acts on the slide on the slide storage box. By means of the mechanism, the problems that in the manual experiment process, in the operation process, steps are tedious, consumed time is long, labor cost is high, experiment operation is difficult to unify, and experiment quality is difficult to guarantee are solved.

Description

Technical Field

[0001] The present invention relates to the field of medical experimental technology, and in particular to a Heinz body detector and a detection method. Background Art

[0002] Heinz bodies are denatured globin bodies attached to red blood cells. Unstable hemoglobin is easily oxidized and denatured to precipitate to form insoluble Heinz bodies. Especially when the patient's red blood cells are incubated in vitro with acetophenylhydrazine peroxidase reagent, a large number of Heinz bodies are generated in the red blood cells. When stained with dyes, Heinz bodies appear in the shape of fine particles, coarse particles or small beads, often located at the edge of red blood cells, of varying sizes, and stained bright purple. They can be used as one of the indicators for identifying unstable hemoglobin.

[0003] At present, the operation steps of Heinz bodies are as follows: take a glass test tube, add 180μl or 3-4 drops of reaction solution, add 25μl or 1 drop of blood, mix, place in a 37.0℃ water bath for 3-5 hours, add a small drop of 2% crystal violet, mix, take a drop of specimen on a slide after 10 minutes, add a cover glass, and observe with an oil immersion lens. There is no automated processing method and equipment for this experiment. The entire experimental process is manually processed, with complicated steps, long time consumption, high labor cost, difficult to unify experimental operations, and difficult to ensure the quality of the experiment. Summary of the invention

[0004] The present invention provides a Heinz body detector and a detection method, which solve the problems of complicated operation steps, long time consumption, high labor cost, difficulty in unifying experimental operations, and difficulty in ensuring the quality of experiments in manual experiments.

[0005] The technical solution of the present invention to solve the above technical problems is as follows:

[0006] A Heinz body detector comprises an incubation system and a detection system, wherein the incubation system comprises a test tube rack storage area, a test tube moving mechanism, a sample transfer mechanism, a sample incubation mechanism and a sample adding mechanism, wherein the incubation system is arranged in an incubation box, the test tube rack storage area is arranged on the left side of the incubation box, the test tube rack storage area is provided with a plurality of test tube rack placement positions, the test tube moving mechanism is slidably arranged at the rear end of the test tube rack storage area, the sample transfer mechanism is arranged at the front end of the test tube rack storage area, the sample incubation mechanism and the sample adding mechanism are arranged on the right side of the incubation box, and the sample adding mechanism is arranged on the sample incubation mechanism;

[0007] The detection system includes an image acquisition system, a stage, a light source control unit, a glass slide moving mechanism, a glass slide storage box, a motion system control unit and an AI intelligent analysis system. The detection system is arranged in the detection box, and the AI ​​intelligent analysis system is electrically connected to the image acquisition system. The lens of the image acquisition system faces the stage, and the stage is movable back and forth and left and right in the detection box through the motion system control unit. The light source control unit is arranged under the stage, and the light source can pass through the stage to illuminate the glass slide. The glass slide storage box is arranged on one side of the glass slide moving mechanism, and the glass slide moving mechanism is slidably arranged on one side of the detection box. The glass slide transfer head of the glass slide moving mechanism acts on the glass slide on the glass slide storage box.

[0008] The beneficial effect of the present invention is that the test tube moving mechanism in the incubation system moves the test tube in the test tube rack storage area to the sample incubation mechanism, the sample adding mechanism adds the sample in the sample transfer mechanism to the test tube, and at the same time, the sample adding mechanism adds other reagents to the test tube with the sample, and incubates it through the sample incubation mechanism. The detection system controls the motion system control unit to move the stage forward and backward, left and right, so that the slide moving mechanism can transfer the slide and cover glass on the slide storage box to the stage, so that the sample to be tested that has been incubated in the sample incubation mechanism can be transferred to the slide on the stage, and the motion system control unit moves the stage forward and backward, left and right, so that the stage moves to the bottom of the image acquisition system, and the light source passes through the stage to irradiate the slide, so that the sample to be tested is collected by the image acquisition system, and the Heinz body is analyzed and detected by the AI ​​intelligent analysis system, so as to realize the automated detection of the experiment, improve the work efficiency of personnel, and standardize the experimental operation, and the quality of the experimental results is guaranteed.

[0009] The present invention can realize accurate sample addition by automated instruments through fully automated accurate sample addition and incubation and accurate detection by the incubation system and the detection system. The sample is mixed with a sample reaction solution with an oxidizing effect in the sample incubation mechanism and an incubation reaction is carried out to oxidize and denature unstable hemoglobin to form insoluble Heinz bodies, which are then dyed purple with a dye, and a high-resolution electron microscope imaging system is used for image acquisition. The Heinz bodies are analyzed and detected by AI analysis technology, thereby realizing automated detection of the experiment, improving work efficiency of personnel, and standardizing experimental operations, thereby ensuring the quality of experimental results.

[0010] On the basis of the above technical solution, the present invention also makes the following improvements.

[0011] Further, the test tube moving mechanism includes a first sliding mechanism, a first telescopic rod, a second telescopic rod and a test tube sleeve, the lower end of the first telescopic rod is fixedly arranged on the first sliding mechanism, the upper end of the first telescopic rod is fixedly connected to one end of the second telescopic rod, and the other end of the second telescopic rod is fixedly connected to the test tube sleeve;

[0012] The sample transfer mechanism includes a sample injection area, a sample buffer area, a sample adding area, a sample rack limiting moving unit, a sample rack, a first conveying structure, a first sensing mechanism, and a first driving mechanism. The sample injection area is arranged on the right side of the sample transfer mechanism, and the sample buffer area is arranged on the left side of the sample transfer mechanism. The sample buffer area includes a first sample buffer area and a second sample buffer area. The sample injection area is connected with the sample adding area through the sample adding area entrance, and the first sample buffer area is connected with the second sample buffer area through the sample buffer area entrance. The first conveying structure is arranged at the bottom of the sample injection area, the sample buffer area, and the sample adding area. A first sensing mechanism and a first driving mechanism are arranged at the bottom of the first conveying structure, a first sensing mechanism is arranged at the entrance of the sample adding area and the entrance of the sample buffer area, the sample rack is slidably connected with the sample injection area, the sample buffer area and the sample rack limiting moving unit; the sample rack limiting moving unit is composed of a PLC controller, a first sample adding hole and a first test tube hole are opened on the top of the sample injection area, a barcode scanner is arranged on the side of the sample injection area and at a position corresponding to the first sample adding hole, a plurality of sample tube sleeves are opened on the top of the sample rack, and barcode scanning ports are opened on the sides of the plurality of sample tube sleeves.

[0013] The beneficial effect of adopting the above further scheme is that the test tube moving mechanism drives the second telescopic rod to slide left and right on the first sliding mechanism through the first telescopic rod. At the same time, the first telescopic rod and the second telescopic rod move up and down, forward and backward, move the test tube sleeve to the test tube rack storage area, and align the test tube. Then, the test tube moving mechanism moves the test tube to the specified position of the sample incubation mechanism; the test tube moving mechanism can reciprocate on the first sliding mechanism to realize the work of automatically and accurately taking the test tube. The sample rack is placed in the sample feeding area, and the sample rack is transferred to the sample adding area through the first sensing mechanism and the first driving mechanism, and the sample information to be tested is transmitted to the incubation system through the barcode scanner. After the sample to be tested in the sample rack is added to the test tube, the sample rack is transferred to the sample buffer area through the first sensing mechanism and the first driving mechanism. The sample transfer mechanism can transfer and place the sample to be tested and the completed test sample separately, which is convenient for the staff to distinguish.

[0014] Furthermore, the sample incubation mechanism includes an incubation chamber, an incubation tray, a reagent storage chamber, a sample mixing mechanism, a second sensing mechanism, a second driving mechanism, an incubation chamber cover, a third driving mechanism and a third sensing mechanism. The incubation tray is arranged in the incubation chamber. A through hole is opened in the center of the incubation tray. The reagent storage chamber passes through the through hole and is placed in the through hole. A reagent position is arranged in the reagent storage chamber. The incubation chamber is provided with a second sensing mechanism and a second driving mechanism. The incubation chamber cover is arranged above the incubation chamber. A second test tube hole, a second sample loading hole and a third sample loading hole are opened on the incubation chamber cover. A reagent storage chamber cover is arranged above the reagent storage chamber. A fourth sample loading hole is opened on the reagent storage chamber cover. The sample mixing mechanism is correspondingly arranged below the third sample loading hole. A third driving mechanism and a third sensing mechanism are arranged below the sample mixing mechanism.

[0015] The beneficial effect of adopting the above further scheme is that a constant temperature is provided for the incubation tray and the reaction sample by the second sensing mechanism provided in the incubation chamber. The test tube of the sample to be tested in the incubation tray is incubated at a constant temperature. After the sample, reaction solution and dye in the test tube are mixed evenly by the third driving mechanism, the test tube of the sample to be tested is placed in the incubation tray for constant temperature incubation, so that the sample, reaction solution and dye react more fully. The second driving mechanism drives the incubation tray to rotate in the incubation chamber, so that the incubated sample rotates to the specified position. The precise addition of samples by the automated instrument is realized, and the sample is mixed with the sample reaction solution with oxidation in the sample incubation mechanism and incubated to react, and the unstable hemoglobin is oxidized and denatured to precipitate to form insoluble Heinz bodies, and then the Heinz bodies are dyed purple by dyeing with dye. The work efficiency of personnel is improved, and the experimental operation is standardized, and the quality of the experimental results is guaranteed.

[0016] Furthermore, the sample loading mechanism has a first sample loading mechanism and a second sample loading mechanism, and the sample loading mechanism includes a lifting rod, a rotating arm, a motor and a sample loading needle. A gear is provided at the lower end of the lifting rod, and the gear at the lower end of the lifting rod is connected to the gear of the motor. The upper end of the lifting rod is fixedly connected to one end of the rotating arm, and the other end of the rotating arm is fixedly connected to the sample loading needle. The upper end of the sample loading needle is connected to a washing liquid tube and a pressure tube, and the ends of the washing liquid tubes are connected to sensing devices.

[0017] The beneficial effect of adopting the above further scheme is that the first sample adding mechanism adds the sucked sample into the test tube to be tested, and the second sample adding mechanism adds the reaction liquid reagent and the dye into the test tube to be tested. After the first sample adding mechanism and the second sample adding mechanism complete the sample adding, the sample adding needles in the first sample adding mechanism and the second sample adding mechanism are cleaned by the washing liquid in the washing liquid bucket, and the waste liquid is discharged into the waste liquid bucket. The sample adding needle is in a clean state, and then the next step is completed. It is prevented that the residual reagent in the sample adding needle is brought into other processes and affects the reaction effect of other reagents.

[0018] Furthermore, an incubation box is arranged on one side of the detection box, an incubation box cover is arranged on the top of the incubation box, the incubation box cover is hinged to one side of the incubation box, a detection box cover is arranged on the top of the detection box, the detection box cover is hinged to one side of the detection box, and one end of the image acquisition system of the detection system is fixedly arranged on the detection box cover.

[0019] The beneficial effect of adopting the above further solution is that by setting an incubation box cover on the top of the incubation box and a detection box cover on the top of the detection box, dust is prevented from entering the incubation system and the detection system, thereby ensuring the cleanliness of the incubation system and the detection system and the accuracy of the detection data.

[0020] Furthermore, the incubation system further includes a second waste disposal device and a washing liquid bucket, the incubation box is divided into two layers, the test tube rack storage area, the test tube moving mechanism, the sample transfer mechanism, the sample incubation mechanism and the sample adding mechanism are arranged on the upper layer of the incubation box, and the second waste disposal device and the washing liquid bucket are arranged on the lower layer of the incubation box;

[0021] The second waste disposal device comprises a second waste storage box and a waste liquid bucket, the lower part of the first test tube hole is connected to the second waste storage box, and the bottom of the sample adding mechanism is connected to the waste liquid bucket and the washing liquid bucket;

[0022] The detection system also includes a first waste disposal device. The detection box is divided into two layers, the image acquisition system, the stage, the slide moving mechanism and the slide storage box are arranged on the upper layer of the detection box, the light source control unit, the first waste disposal device and the motion system control unit are arranged on the lower layer of the detection box, the slide storage box is arranged on the first waste disposal device, and a waste slide placement port is opened at the top of one end of the first waste disposal device, and the waste slide placement port is located behind the slide storage box.

[0023] The beneficial effect of adopting the above further solution is that the sample adding mechanism is cleaned by the washing liquid in the washing liquid bucket, and the waste liquid and the sample test tube after the test are transferred to the second waste disposal device. The sample adding mechanism is kept clean, ensuring that the sample adding mechanism will not carry the sample reagent from the previous time when it works next time, thereby ensuring the accuracy of the test data.

[0024] Furthermore, the image acquisition system includes a lens, an image collector, a focusing ring, a fixed plate, a fifth telescopic rod, and a second transmission structure. The top of the fifth telescopic rod is fixedly arranged on the top of the detection box, the bottom end of the fifth telescopic rod is fixedly connected to the fixed plate, a second transmission structure is arranged at the bottom of the fixed plate, the second transmission structure acts on the focusing ring, the lens is rotatably connected to the image collector through the focusing ring, the upper end of the image collector is fixedly connected to the fixed plate, and the lower end of the image collector is threadedly connected to the focusing ring.

[0025] The beneficial effect of adopting the above further scheme is that the image acquisition system is a high-resolution electron microscope imaging system, which is composed of a high-power lens and an image collector. The high-power lens is rotatably connected to the image collector through a focusing ring, so that red blood cells and Heinz bodies can be magnified; and the focusing ring is acted on by the second transmission structure for fine focusing, and finally the collected high-definition images of red blood cells and Heinz bodies are transmitted to the system.

[0026] Further, the stage includes a first support plate, a second support plate, and a third support plate, the first support plate is arranged above the light source control unit and the first waste disposal device, the second support plate is arranged on the first support plate for sliding left and right, the third support plate is arranged on the second support plate for sliding front and back, the bottom plate of the first support plate is provided with a light source entrance, the bottom plate of the second support plate is provided with a light transmission area, and the third support plate is provided with a plurality of glass slide placement areas, and the light source of the light source control unit can be projected onto the glass slide placement area through the light source entrance and the light transmission area;

[0027] The motion system control unit includes a first motor, a first screw, a first screw fixing plate, a second motor, a second screw and a second screw fixing plate. The first screw fixing plates are respectively arranged at both ends of the first support plate, and the two ends of the first screw are respectively connected to the two first screw fixing plates. One end of the first screw passes through a screw connecting hole provided on the second support plate, and one end of the first screw is connected to the first motor. The second support plate is arranged on the first support plate for sliding left and right through the first screw. The second screw fixing plate and the second motor are arranged at a position of the second support plate away from the light-transmitting area. A nut is also arranged on the second screw fixing plate, and the output end of the second motor is connected to the nut. The second screw is arranged behind the third support plate, and one end of the second screw is screwed to the nut.

[0028] The beneficial effect of adopting the above further scheme is that the detection system controls the motion system control unit to move the stage forward, backward, left and right, so that the slide moving mechanism can transfer the slides and cover slides on the slide storage box to the stage, so that the samples to be tested that have been incubated in the sample incubation mechanism can be transferred to the slide on the stage, and the motion system control unit moves the stage forward, backward, left and right, so that the stage moves to the bottom of the image acquisition system, and the light source passes through the stage to illuminate the slide, so that the sample to be tested is collected data by the image acquisition system, and analyzed and detected by the AI ​​intelligent analysis system to detect Heinz bodies, thereby realizing automated detection of the experiment, improving personnel work efficiency, and standardizing experimental operations, and ensuring the quality of experimental results.

[0029] Furthermore, the slide storage box is provided with a plurality of slide storage chambers and a plurality of cover glass storage chambers, the slide storage chambers are larger than the cover glass storage chambers, the slide storage chambers and the cover glass storage chambers are arranged in a convex hollow shape, and a waste slide placement opening is opened at the top of one end of the first waste disposal device, and the waste slide placement opening is located at the back of the slide storage box;

[0030] The slide moving mechanism includes a third telescopic rod, a fourth telescopic rod, a second sliding mechanism and a slide transfer head. One end of the third telescopic rod is fixedly connected to the top of the fourth telescopic rod, and the other end is connected to the slide transfer head. The fourth telescopic rod is a telescopic structure, and the bottom end of the fourth telescopic rod is slidably arranged on the second sliding mechanism.

[0031] The beneficial effect of adopting the above further scheme is that slides are stored in the slide storage chamber, and cover slips are stored in the cover slip storage chamber. The slides and cover slips are respectively transferred to multiple slide placement areas set on the third support plate by the slide moving mechanism, so that multiple slides can carry samples to be tested and are used for detection and collection one by one. The samples to be tested are collected by the image acquisition system, and the Heinz bodies are analyzed and detected by the AI ​​intelligent analysis system, so as to realize the automated detection of the experiment, improve the work efficiency of personnel, standardize the experimental operation, and ensure the quality of the experimental results.

[0032] On the other hand, a Heinz body detection method is based on a Heinz body detector, and the detection method is:

[0033] S1: The Heinz body detector is automatically reset, and all system components complete various preparations before the test;

[0034] S2: placing the sample to be tested into the sample tube sleeve of the sample rack, and engaging and slidingly connecting the sample rack with the sample transfer mechanism, the first driving mechanism drives the first conveying structure to move the sample rack, and the sample rack limit moving unit controls the sample rack to move between the sample injection area and the sample addition area;

[0035] S3: Scan the barcode of the sample to be tested by a barcode scanner, and transmit the information of the sample to be tested to the incubation system. The incubation system controls the test tube moving mechanism to work, and the test tube moving mechanism transfers the test tube from the test tube rack storage area to the sample incubation mechanism;

[0036] S4: the second sensing mechanism of the sample incubation mechanism senses that the test tube enters the incubation chamber, and the second driving mechanism drives the incubation plate to rotate;

[0037] S5: the first sample adding mechanism adds the sample into the test tube, and then the sample adding needle of the first sample adding mechanism is cleaned with a washing solution;

[0038] S6: the second sample adding mechanism adds the reaction liquid reagent into the test tube, mixes the sample in the test tube with the reaction liquid reagent evenly and incubates them, and then uses the washing solution to clean the sample adding needle of the second sample adding mechanism;

[0039] S7: After all samples to be tested are added to the sample rack, the sample rack moves to a designated position in the sample buffer area;

[0040] S8: After the sample in the test tube and the reaction liquid reagent are mixed evenly and incubated, the dye of the second sample adding mechanism is added into the test tube, the sample to be tested, the reaction liquid reagent and the sample of the dye in the test tube are mixed evenly and incubated, and then the sample adding needle of the second sample adding mechanism is cleaned with a washing solution;

[0041] S9: the motion system control unit of the detection system controls the movement of the stage and starts the slide moving mechanism, and the slide moving mechanism places the slide on the slide storage box at a designated position of the slide placement area on the third support plate;

[0042] S10: After the sample incubation is completed, the second driving mechanism drives the incubation plate to rotate, and the second loading mechanism samples the sample to be tested and adds the sample to the slide; then, the loading needle of the second loading mechanism is cleaned with a washing solution;

[0043] S11: After the reagent incubated in the sample test tube to be tested is sampled and tested, the test tube moving mechanism transfers the test tube to the second waste storage box;

[0044] S12: The motion system control unit of the detection system controls the movement of the stage and starts the slide moving mechanism, and the slide moving mechanism transfers the cover glass on the slide storage box to the sample on the slide and covers the sample;

[0045] S13: The motion system control unit of the detection system controls the stage to move to the bottom of the image acquisition system, aligns the sample slide to be detected with the image acquisition system, and the image acquisition system acquires an image of the sample slide and uploads the acquired image to the AI ​​intelligent analysis system;

[0046] S14: Repeat steps S1-S13 multiple times according to the requirements of the test, perform multiple image acquisitions, and analyze the results;

[0047] S15: After the sample analysis is completed, the motion system control unit of the detection system controls the movement of the stage, and the slide moving mechanism transfers the tested slide and cover glass on the stage to the waste slide placement port, and transfers the slide and cover glass to the first waste disposal device through the waste slide placement port;

[0048] S16: The second driving mechanism drives the incubation plate to rotate, and the test tube moving mechanism moves the tested test tube out of the incubation chamber, and transfers the tested sample test tube to the second waste storage box.

[0049] The beneficial effect of adopting the above further scheme is that the present invention can realize accurate sample addition by automated instruments through fully automated accurate sample addition and incubation and accurate detection of the incubation system and the detection system. The sample is mixed with a sample reaction solution with an oxidizing effect in the sample incubation mechanism and an incubation reaction is carried out to oxidize and denature the unstable hemoglobin to form insoluble Heinz bodies, which are then stained with a dye to be dyed purple, and a high-resolution electron microscope imaging system is further used for image acquisition. The Heinz bodies are analyzed and detected by AI analysis technology, thereby realizing automated detection of the experiment, improving the work efficiency of personnel, and standardizing the experimental operations, and ensuring the quality of the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] Figure 1 It is a structural schematic diagram of a Heinz body detector of the present invention;

[0051] Figure 2 It is a structural schematic diagram of an incubation system of a Heinz body detector of the present invention;

[0052] Figure 3 This is a schematic diagram of the structure of an incubation structure of a Heinz body detector of the present invention;

[0053] Figure 4 This is a schematic diagram of the structure of an incubation plate of a Heinz body detector of the present invention;

[0054] Figure 5 This is a schematic diagram of the structure of an incubation chamber of a Heinz body detector of the present invention;

[0055] Figure 6 This is a schematic diagram of the structure of an incubation chamber cover of a Heinz body detector of the present invention;

[0056] Figure 7 It is a structural schematic diagram of a reagent storage chamber cover of a Heinz body detector of the present invention;

[0057] Figure 8 It is a structural schematic diagram of a sample transfer mechanism of a Heinz body detector of the present invention;

[0058] Fig. 9 It is a structural schematic diagram of a sample rack of a Heinz body detector of the present invention;

[0059] Fig.10 It is a structural schematic diagram of a sample adding mechanism of a Heinz body detector of the present invention;

[0060] Fig.11 This is a schematic structural diagram of a test tube sleeve of a Heinz body detector of the present invention;

[0061] Fig.12It is a structural schematic diagram of a test tube moving mechanism and a glass slide moving mechanism of a Heinz body detector of the present invention;

[0062] Fig.13 It is a structural schematic diagram of a Heinz body detector detection system of the present invention;

[0063] Fig.14 It is a structural schematic diagram of an image acquisition system of a Heinz body detector of the present invention;

[0064] Fig.15 It is a structural schematic diagram of a stage of a Heinz body detector of the present invention;

[0065] Fig.16 This is a schematic structural diagram of a first support plate of a Heinz body detector according to the present invention;

[0066] Fig.17 This is a schematic structural diagram of a second support plate of a Heinz body detector according to the present invention;

[0067] Fig.18 This is a schematic structural diagram of a third support plate of a Heinz body detector of the present invention;

[0068] Fig.19 This is a structural schematic diagram of a slide storage box for a Heinz body detector of the present invention;

[0069] Fig. 20 It is a structural schematic diagram of a slide transfer head of a Heinz body detector of the present invention;

[0070] Fig.21 The present invention is a flow chart of a method for detecting Heinz bodies.

[0071] In the accompanying drawings, the components represented by the reference numerals are as follows: 1. incubator; 10. test tube rack storage area; 101. test tube rack placement position; 11. incubator cover; 12. test tube moving mechanism; 121. first sliding mechanism; 124. first telescopic rod; 125. second telescopic rod; 126. test tube sleeve; 1261. test tube sleeve mouth; 1262. test tube clamp; 1263. test tube clamp telescopic rod; 1264. clamping member; 1265. clamping member support rod; 1266. spring; 1267. induction detection device; 13. sample transfer mechanism; 131. sample adding area; 1311. first sample adding hole; 1312. first test tube hole; 1313. barcode scanner; 132. sample buffer area; 13 21. First sample buffer area; 13211. Sample loading area entrance; 13213. Sample buffer area entrance; 1322. Second sample buffer area; 133. Sample loading area; 134. Sample rack limit moving unit; 135. Sample rack; 1351. Sample tube sleeve; 1352. Barcode scanning port; 136. First transmission structure; 137. First sensing mechanism; 14. Sample incubation mechanism; 141. Incubation chamber; 142. Incubation tray; 143. Reagent storage chamber; 1431. Reagent position; 1432. Reagent storage chamber cover; 1433. Fourth sample loading hole; 144. Sample mixing mechanism; 147. Incubation chamber cover; 1471. Second test tube hole; 1472. Second sample loading hole; 1473 , the third sample loading hole; 15, the sample loading mechanism; 151, the first sample loading mechanism; 152, the second sample loading mechanism; 153, the lifting rod; 154, the rotating arm; 155, the sample loading needle; 16, the second waste disposal device; 161, the second waste storage box; 162, the waste liquid bucket; 17, the washing liquid bucket; 2, the detection box; 21, the image acquisition system; 211, the lens; 212, the image acquisition device; 213, the focusing ring; 214, the fixing plate; 215, the fifth telescopic rod; 216, the second transmission structure; 22, the stage; 221, the first support plate; 2211, the light source entrance; 222, the second support plate; 2221, the light transmission area; 223, the third support plate; 2231, the slide placement area; 23. Light source control unit; 24. Glass slide moving mechanism; 241. Third telescopic rod; 242. Fourth telescopic rod; 243. Second sliding mechanism; 244. Glass slide transfer head; 2441. Transfer head inner cavity; 2442. Suction cup support rod; 2443. Fixed plate support rod; 2444. Fixed plate; 2445. Suction cup; 25. Glass slide storage box; 251. Glass slide storage chamber; 252. Cover glass storage chamber; 26. First waste disposal device; 261. Waste glass slide placement port; 27. Motion system control unit; 271. First motor; 272. First screw; 273. First screw fixing plate; 274. Second motor; 275. Second screw; 276. Second screw fixing plate. DETAILED DESCRIPTION

[0072] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0073] Example 1

[0074] like Figure 1-20 As shown, a Heinz body detector includes an incubation system and a detection system, the incubation system includes a test tube rack storage area 10, a test tube moving mechanism 12, a sample transfer mechanism 13, a sample incubation mechanism 14 and a sample adding mechanism 15, the incubation system is arranged in an incubation box 1, the test tube rack storage area 10 is arranged on the left side of the incubation box 1, the test tube rack storage area 10 is provided with a plurality of test tube rack placement positions 101, the test tube moving mechanism 12 is slidably arranged at the rear end of the test tube rack storage area 10, the sample transfer mechanism 13 is arranged at the front end of the test tube rack storage area 10, the sample incubation mechanism 14 and the sample adding mechanism 15 are arranged on the right side of the incubation box 1, and the sample adding mechanism 15 is arranged on the sample incubation mechanism 14;

[0075] The detection system includes an image acquisition system 21, a stage 22, a light source control unit 23, a glass slide moving mechanism 24, a glass slide storage box 25, a motion system control unit 27 and an AI intelligent analysis system. The detection system is arranged in the detection box 2, and the AI ​​intelligent analysis system is electrically connected to the image acquisition system 21. The lens of the image acquisition system 21 faces the stage 22. The stage 22 is movably arranged in the detection box 2 front and back and left and right through the motion system control unit 27. The light source control unit 23 is arranged under the stage 22, and the light source can pass through the stage 22 to illuminate the glass slide. The glass slide storage box 25 is arranged on one side of the glass slide moving mechanism 24. The glass slide moving mechanism 24 is slidably arranged on one side of the detection box 2, and the glass slide transfer head 244 of the glass slide moving mechanism 24 acts on the glass slide on the glass slide storage box 25.

[0076] Specifically, in the incubation system, the test tube moving mechanism moves the test tube in the test tube rack storage area to the sample incubation mechanism, and the sample adding mechanism adds the sample in the sample transfer mechanism into the test tube. At the same time, the sample adding mechanism adds other reagents into the test tube with the sample, and incubates it through the sample incubation mechanism.

[0077] The detection system controls the motion system control unit 27 to move the stage 22 forward and backward and left and right. The stage 22 moves to one side of the slide storage box 25. The slide moving mechanism 24 can transfer the slide on the slide storage box 25 to the stage 22. Then, the motion system control unit 27 controls the stage 22 to move toward the sample incubation mechanism 14. The second loading mechanism 152 mixes the sample, reaction solution and dye in the test tube from the third loading hole 1473 and places the sample to be tested after incubation on the slide of the stage 22. The motion system control unit 27 controls the stage 22 to move toward the slide storage box 25. Then, the slide moving mechanism 24 can transfer the cover glass on the slide storage box 25 to the stage 22, and cover the cover glass on the slide with the sample to be tested. Then, the motion system control unit 27 controls the stage 22 to move to the bottom of the image acquisition system 21, and the light source passes through the stage 22 to illuminate the glass slide. The sample to be tested is collected data through the image acquisition system 21, and the Heinz body is analyzed and detected by the AI ​​intelligent analysis system.

[0078] The present invention can realize accurate sample addition by automated instruments through fully automated accurate sample addition and incubation and accurate detection of the incubation system and the detection system. The sample is mixed with a sample reaction solution with an oxidizing effect in the sample incubation mechanism 14 and an incubation reaction is carried out to oxidize and denature unstable hemoglobin to form insoluble Heinz bodies, which are then stained with a dye to be dyed purple. A high-resolution electron microscope imaging system is further used for image acquisition, and the Heinz bodies are analyzed and detected by AI analysis technology, thereby realizing automated detection of the experiment, improving the work efficiency of personnel, and standardizing the experimental operation, and ensuring the quality of the experimental results.

[0079] like Figure 1-20 As shown, the test tube moving mechanism 12 includes a first sliding mechanism 121, a first telescopic rod 124, a second telescopic rod 125 and a test tube sleeve 126, the lower end of the first telescopic rod 124 is fixedly arranged on the first sliding mechanism 121, the upper end of the first telescopic rod 124 is fixedly connected to one end of the second telescopic rod 125, and the other end of the second telescopic rod 125 is fixedly connected to the test tube sleeve 126;

[0080] The sample transfer mechanism 13 includes a sample injection area 133, a sample buffer area 132, a sample adding area 131, a sample rack limiting moving unit 134, a sample rack 135, a first conveying structure 136, a first sensing mechanism 137, and a first driving mechanism. The sample injection area 133 is arranged on the right side of the sample transfer mechanism 13, and the sample buffer area 132 is arranged on the left side of the sample transfer mechanism 13. The sample buffer area 132 includes a first sample buffer area 1321 and a second sample buffer area 1322. The sample injection area 133 is connected to the sample adding area 131 through a sample adding area inlet 13211, and the first sample buffer area 1321 is connected to the second sample buffer area 1322 through a sample buffer area inlet 13213. The bottom of the sample injection area 133, the sample buffer area 132, and the sample adding area 131 are all provided with a first conveying mechanism. Structure 136, a first sensing mechanism 137 and a first driving mechanism are arranged at the bottom of the first conveying structure 136, a first sensing mechanism 137 is arranged at the entrance 13211 of the sample adding area and the entrance 13213 of the sample buffer area, a sample rack 135 is slidably connected with the sample injection area 133, the sample buffer area 132 and the sample rack limiting moving unit 134; the sample rack limiting moving unit 134 is composed of a PLC controller, a first sample adding hole 1311 and a first test tube hole 1312 are opened at the top of the sample injection area 133, a barcode scanner 1313 is arranged on the side of the sample injection area 133 and at a position corresponding to the first sample adding hole 1311, a plurality of sample tube sleeves 1351 are opened at the top of the sample rack 135, and a barcode scanning port 1352 is opened on the side of the plurality of sample tube sleeves 1351.

[0081] Specifically, the sample to be tested is placed in the sample tube sleeve 1351 of the sample rack 135, the barcode of the sample to be tested is facing the barcode scanning port 1352, the sample rack 135 is placed in the sample injection area 133, the card slot on the sample rack 135 is engaged and slidably connected with the card strip set in the sample transfer mechanism 13, and the sample rack 135 is transferred to the sample adding area 131 through the first sensing mechanism 137 and the first driving mechanism, and the barcode scanner 1313 transmits the sample information to be tested into the incubation system. After the sample to be tested in the sample rack 135 is added to the test tube, the sample rack 135 is transferred from the sample adding area entrance 13211 to the first sample buffer area 1321 through the first sensing mechanism 137 and the first driving mechanism. When the first sample buffer area 1321 is full of the sample rack 135, the sample rack 135 is transferred from the sample buffer area entrance 13213 to the second sample buffer area 1322. Finally, the staff moves the test tube rack that has completed the test out of the sample buffer area 132. In this embodiment, the first sensing mechanism 137 is provided at the sample injection area 133, the sample buffer area 132, the sample adding area 131, the sample rack position limiting moving unit 134, the sample adding area entrance 13211 and the sample buffer area entrance 13213. The first conveying structure 136 is provided at the bottom of the sample injection area 133 and the sample buffer area 132. The first conveying structure 136 and the bottom of the sample rack position limiting moving unit 134 are both provided with a first driving mechanism. The sample adding area entrance 13211 and the sample buffer area entrance 13213 are both provided with a sample rack position limiting moving unit 134.

[0082] The test tube moving mechanism 12 drives the second telescopic rod 125 to slide left and right on the first sliding mechanism 121 through the first telescopic rod 124. At the same time, the first telescopic rod 124 and the second telescopic rod 125 move up and down and forward and backward to move the test tube sleeve 126 to the test tube rack storage area 10 and align the test tube. Then, the test tube moving mechanism 12 moves the test tube to the specified position of the sample incubation mechanism 14; the test tube moving mechanism 12 can reciprocate on the first sliding mechanism 121 to realize the work of automatically and accurately taking the test tube.

[0083] In this embodiment, the first sliding mechanism 121 includes a slide rail, a threaded slider, a screw, a motor, and a mechanism gear. One end of the screw is connected to the motor and the mechanism gear, and both ends of the screw are respectively arranged on the slide rail. The threaded slider is fixedly arranged on the slide rail, and the lower end of the first telescopic rod 124 is fixedly arranged on the threaded slider; the screw rotates forward or reversely under the drive of the motor, and the screw rotates while driving the threaded slider to slide in the slide rail, and drives the first telescopic rod 124, the second telescopic rod 125 and the test tube sleeve 126 to move forward or reversely.

[0084] In this embodiment, the test tube sleeve 126 includes a test tube sleeve opening 1261, a test tube clamp 1262, a test tube clamp telescopic rod 1263, a clamping piece 1264, a clamping piece support rod 1265, a spring 1266 and an induction detection device 1267. The interior of the test tube sleeve 126 is a hollow trapezoid with a smaller upper end and a larger lower end. The lower end is the test tube sleeve opening 1261. The test tube clamp telescopic rod 1263 is arranged on the top of the test tube clamp 1262, and two clamping piece support rods 1265 are arranged at the bottom of the test tube clamp 1262. A clamping piece 1264 is respectively arranged at the bottom of the two clamping piece support rods 1265. The two ends of the spring 1266 are respectively connected to the clamping piece support rods 1265 on both sides, and the side wall of the test tube sleeve opening 1261 is also provided with an induction detection device 1267.

[0085] There is a sensing detection device 1267 inside the test tube sleeve 126. When the first telescopic rod 124 and the test tube sleeve 126 move downward, the test tube enters the test tube sleeve 126 through the test tube sleeve opening 1261. The sensing detection device 1267 senses the test tube and transmits a signal to the system, thereby controlling the first telescopic rod 124 to stop moving downward and controlling the test tube clamp telescopic rod 1263 to move upward.

[0086] like Figure 1-20 As shown, the sample incubation mechanism 14 includes an incubation chamber 141, an incubation tray 142, a reagent storage chamber 143, a sample mixing mechanism 144, a second sensing mechanism, a second driving mechanism, an incubation chamber cover 147, a third driving mechanism and a third sensing mechanism. The incubation tray 142 is arranged in the incubation chamber 141. A through hole is opened in the center of the incubation tray 142. The reagent storage chamber 143 passes through the through hole and is placed in the through hole. A reagent position 1431 is arranged in the reagent storage chamber 143. The second sensing mechanism is arranged in the incubation chamber 141. and a second driving mechanism, an incubation chamber cover 147 is arranged above the incubation chamber 141, and a second test tube hole 1471, a second sample loading hole 1472 and a third sample loading hole 1473 are provided on the incubation chamber cover 147; a reagent storage chamber cover 1432 is arranged above the reagent storage chamber 143, and a fourth sample loading hole 1433 is provided on the reagent storage chamber cover 1432; the sample mixing mechanism 144 is correspondingly arranged below the third sample loading hole 1473, and a third driving mechanism and a third sensing mechanism are provided below the sample mixing mechanism 144.

[0087] Specifically, a constant temperature is provided for the incubation tray 142 and the reaction sample by a second sensing mechanism provided in the incubation chamber 141. In the present embodiment, the second sensing mechanism is a temperature control device and a temperature sensing device. After the test tube moving mechanism 12 transfers the test tube to the second test tube hole 1471 of the incubation tray 142, the second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the second sample addition hole 1472. The sample addition mechanism 15 draws the sample to be tested from the first sample addition hole 1311, and adds it to the test tube to be tested through the second sample addition hole 1472. The second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the third sample addition hole 1473. The sample addition mechanism 15 adds the reaction liquid reagent in the fourth sample addition hole 1433 to the test tube to be tested below the third sample addition hole 1473. Then, the test tube of the sample to be tested is mixed evenly with the sample and the reaction liquid reagent through the third driving mechanism, and then placed in the incubation tray 142 for constant temperature incubation at 37.0°C for 3.5 hours. After the incubation is completed, the sample adding mechanism 15 adds the dye in the fourth sample adding hole 1433 to the test tube to be tested under the third sample adding hole 1473. The test tube of the sample to be tested is mixed evenly with the sample, the reaction liquid and the dye in the test tube through the third driving mechanism, and then continues to incubate for 10 minutes to complete the incubation.

[0088] like Figure 1-20 As shown, the sample loading mechanism 15 has a first sample loading mechanism 151 and a second sample loading mechanism 152. The sample loading mechanism 15 includes a lifting rod 153, a rotating arm 154, a motor and a sample loading needle 155. The lower end of the lifting rod 153 is provided with a gear, and the gear at the lower end of the lifting rod 153 is connected to the gear of the motor. The upper end of the lifting rod 153 is fixedly connected to one end of the rotating arm 154, and the other end of the rotating arm 154 is fixedly connected to the sample loading needle 155. The upper end of the sample loading needle 155 is connected to a washing liquid tube and a pressure tube, and the ends of the washing liquid tubes are connected to sensing devices.

[0089] Specifically, the second driving mechanism drives the incubation disk 142 to rotate, and transfers the test tube to be tested to the bottom of the second sample loading hole 1472. The first sample loading mechanism 151 absorbs the sample to be tested from the first sample loading hole 1311 and adds it to the test tube to be tested through the second sample loading hole 1472. The second driving mechanism drives the incubation disk 142 to rotate, and transfers the test tube to be tested to the bottom of the third sample loading hole 1473. The second sample loading mechanism 152 adds the reaction liquid reagent in the fourth sample loading hole 1433 to the test tube to be tested below the third sample loading hole 1473. Then, the test tube of the sample to be tested is mixed evenly with the sample and the reaction liquid reagent through the third driving mechanism, and then placed in the incubation tray 142 for constant temperature incubation at 37.0°C for 3.5 hours. After the incubation is completed, the second loading mechanism 152 adds the dye in the fourth loading hole 1433 to the test tube to be tested under the third loading hole 1473. The test tube of the sample to be tested is mixed evenly with the sample, the reaction liquid and the dye through the third driving mechanism, and then continues to incubate for 10 minutes to complete the incubation.

[0090] After the first sample loading mechanism 151 and the second sample loading mechanism 152 have completed loading, the loading needles 155 in the first sample loading mechanism 151 and the second sample loading mechanism 152 are cleaned by the washing liquid in the washing liquid barrel 17 , and the waste liquid is discharged into the waste liquid barrel 162 .

[0091] like Figure 1-20 As shown, the incubation box 1 is arranged on one side of the detection box 2, an incubation box cover 11 is arranged on the top of the incubation box 1, and the incubation box cover 11 is hinged to one side of the incubation box 1, a detection box cover is arranged on the top of the detection box 2, and the detection box cover is hinged to one side of the detection box 2, and one end of the image acquisition system 21 of the detection system is fixedly arranged on the detection box cover.

[0092] Specifically, by setting an incubation box cover 11 on the top of the incubation box 1 and a detection box cover on the top of the detection box 2, dust is prevented from entering the incubation system and the detection system, thereby ensuring the cleanliness of the incubation system and the detection system and the accuracy of the detection data.

[0093] like Figure 1-20 As shown, the incubation system further includes a second waste disposal device 16 and a washing liquid bucket 17, and the incubation box 1 is divided into an upper and lower layer, the test tube rack storage area 10, the test tube moving mechanism 12, the sample transfer mechanism 13, the sample incubation mechanism 14 and the sample adding mechanism 15 are arranged on the upper layer of the incubation box 1, and the second waste disposal device 16 and the washing liquid bucket 17 are arranged on the lower layer of the incubation box 1;

[0094] The second waste disposal device 16 includes a second waste storage box 161 and a waste liquid barrel 162. The lower part of the first test tube hole 1312 is connected to the second waste storage box 161. The bottom of the sample adding mechanism 15 is connected to the waste liquid barrel 162 and the washing liquid barrel 17.

[0095] The detection system also includes a first waste disposal device 26. The detection box 2 is divided into two layers, the upper and lower layers. The image acquisition system 21, the stage 22, the glass slide moving mechanism 24 and the glass slide storage box 25 are arranged on the upper layer of the detection box 2. The light source control unit 23, the first waste disposal device 26 and the motion system control unit 27 are arranged on the lower layer of the detection box 2. The glass slide storage box 25 is arranged on the first waste disposal device 26. A waste glass slide placement port 261 is opened at the top of one end of the first waste disposal device 26, and the waste glass slide placement port 261 is located behind the glass slide storage box 25.

[0096] Specifically, the sample adding mechanism is cleaned with the washing liquid in the washing liquid bucket, and the waste liquid and the sample test tube after the test are transferred to the second waste disposal device. The sample adding mechanism is kept clean to ensure that the sample adding mechanism will not carry the sample reagent from the previous time when it works next time, thereby ensuring the accuracy of the test data.

[0097] like Figure 1-20 As shown, the image acquisition system 21 includes a lens 211, an image collector 212, a focusing ring 213, a fixing plate 214, a fifth telescopic rod 215, and a second conveying structure 216. The top of the fifth telescopic rod 215 is fixedly arranged on the top of the detection box 2, the bottom end of the fifth telescopic rod 215 is fixedly connected to the fixing plate 214, the bottom of the fixing plate 214 is provided with a second conveying structure 216, the second conveying structure 216 acts on the focusing ring 213, the lens 211 is rotatably connected to the image collector 212 through the focusing ring 213, the upper end of the image collector 212 is fixedly connected to the fixing plate 214, and the lower end of the image collector 212 is threadedly connected to the focusing ring 213.

[0098] Specifically, in the present embodiment, the image acquisition system 21 is a high-resolution electron microscope imaging system, and the lens 211 adopts a high-power lens 211, and the high-power lens 211 is rotatably connected to the image acquisition device 212 via a focusing ring 213. By rotating and focusing the focusing ring 213, the red blood cells and Heinz bodies can be magnified; and the second transmission structure 216 acts on the focusing ring 213 for fine focusing, and finally the collected high-definition images of the red blood cells and Heinz bodies are transmitted to the system.

[0099] like Figure 1-20As shown, the stage 22 includes a first support plate 221, a second support plate 222, and a third support plate 223. The first support plate 221 is arranged above the light source control unit 23 and the first waste disposal device 26. The second support plate 222 is arranged on the first support plate 221 for sliding left and right. The third support plate 223 is arranged on the second support plate 222 for sliding front and back. A light source entrance 2211 is provided on the bottom plate of the first support plate 221. A light transmission area 2221 is provided on the bottom plate of the second support plate 222. A plurality of glass slide placement areas 2231 are provided on the third support plate 223. The light source of the light source control unit 23 can be projected onto the glass slide placement area 2231 through the light source entrance 2211 and the light transmission area 2221.

[0100] The motion system control unit 27 includes a first motor 271, a first screw 272, a first screw fixing plate 273, a second motor 274, a second screw 275 and a second screw fixing plate 276. The first support plate 221 is provided with a first screw fixing plate 273 at both ends, and the first screw 272 is connected to the two first screw fixing plates 273 at both ends. One end of the first screw 272 passes through the screw connecting hole provided on the second support plate 222, and the first screw 272 is provided with a second screw fixing plate 276. One end is connected to the first motor 271, the second support plate 222 is slidably arranged on the first support plate 221 through the first screw 272, the second support plate 222 is provided with a second screw fixing plate 276 and a second motor 274 at a position away from the light-transmitting area 2221, a nut is also provided on the second screw fixing plate 276, the output end of the second motor 274 is connected to the nut, the second screw 275 is provided behind the third support plate 223, and one end of the second screw 275 is screwed to the nut.

[0101] Specifically, the detection system controls the motion system control unit 27 to move the stage 22 forward and backward and left and right. In this embodiment, the motion system control unit 27 controls the first screw 272 to rotate and drive through the first motor 271, controls the second support plate 222 to slide left and right on the first support plate 221, and the motion system control unit 27 controls the second screw 275 to rotate and drive through the second motor 274, controls the third support plate 223 to slide forward and backward on the second support plate 222.

[0102] The stage 22 moves to one side of the slide storage box 25, and the slide moving mechanism 24 can transfer the slide on the slide storage box 25 to the stage 22. Then, the motion system control unit 27 controls the stage 22 to move toward the sample incubation mechanism 14, and the second loading mechanism 152 mixes the sample, reaction solution, and stain in the test tube from the third loading hole 1473 and places the sample to be tested after incubation on the slide on the stage 22. The motion system control unit 27 controls the stage 22 to move toward the slide storage box 25, and then, the slide moving mechanism 24 can transfer the cover glass on the slide storage box 25 to the stage 22, and cover the cover glass on the slide with the sample to be tested. Then, the motion system control unit 27 controls the stage 22 to move to the bottom of the image acquisition system 21, and the light source passes through the stage 22 to illuminate the glass slide. The sample to be tested is collected data through the image acquisition system 21, and the Heinz body is analyzed and detected by the AI ​​intelligent analysis system.

[0103] like Figure 1-20 As shown, the slide storage box 25 is provided with a plurality of slide storage chambers 251 and a plurality of cover glass storage chambers 252, the slide storage chambers 251 are larger than the cover glass storage chambers 252, the slide storage chambers 251 and the cover glass storage chambers 252 are arranged in a convex hollow shape, and a waste slide placement opening 261 is opened at the top of one end of the first waste disposal device 26, and the waste slide placement opening 261 is located at the back of the slide storage box 25;

[0104] The slide moving mechanism 24 includes a third telescopic rod 241, a fourth telescopic rod 242, a second sliding mechanism 243 and a slide transfer head 244. One end of the third telescopic rod 241 is fixedly connected to the top of the fourth telescopic rod 242, and the other end is connected to the slide transfer head 244. The fourth telescopic rod 242 is a telescopic structure, and the bottom end of the fourth telescopic rod 242 is slidably set on the second sliding mechanism 243.

[0105] Specifically, slides are stored in the slide storage chamber 251, and cover slides are stored in the cover slide storage chamber 252. The slides and cover slides are respectively transferred to the multiple slide placement areas 2231 set on the third support plate 223 by the slide moving mechanism 24, so that multiple slides can carry samples to be tested and are used for detection and collection one by one. The samples to be tested are collected data through the image acquisition system 21, and the Heinz bodies are analyzed and detected by the AI ​​intelligent analysis system, so as to realize the automated detection of the experiment, improve the work efficiency of the personnel, standardize the experimental operation, and ensure the quality of the experimental results.

[0106] In this embodiment, the slide moving mechanism 24 includes a third telescopic rod 241, a fourth telescopic rod 242, a second sliding mechanism 243 and a slide transfer head 244. The lower end of the third telescopic rod 241 is fixedly disposed on the second sliding mechanism 243, the upper end of the third telescopic rod 241 is fixedly connected to one end of the fourth telescopic rod 242, and the other end of the fourth telescopic rod 242 is fixedly connected to the slide transfer head 244.

[0107] In this embodiment, the second sliding mechanism 243 includes a slide rail, a threaded slider, a screw, a motor, and a mechanism gear. One end of the screw is connected to the motor and the mechanism gear, and both ends of the screw are respectively arranged on the slide rail. The threaded slider is fixedly arranged on the slide rail, and the lower end of the third telescopic rod 241 is fixedly arranged on the threaded slider; the screw rotates forward or reversely under the drive of the motor, and while the screw rotates, it drives the threaded slider to slide in the slide rail, and drives the third telescopic rod 241, the fourth telescopic rod 242 and the slide transfer head 244 to move forward or reversely.

[0108] In this embodiment, the glass slide transfer head 244 includes a transfer head inner cavity 2441, a suction cup support rod 2442, a motor, a fixed plate support rod 2443, a fixed plate 2444, and a suction cup 2445. The top end of the suction cup support rod 2442 is connected to the top of the transfer head inner cavity 2441, and the bottom end of the suction cup support rod 2442 is provided with a suction cup 2445. Multiple motors are evenly distributed in the transfer head inner cavity 2441, and the output ends of the multiple motors are all connected to the fixed plate support rod 2443. The bottoms of the multiple fixed plate support rods 2443 are all provided with fixed plates 2444. One end of the fixed plate support rod 2443 provided with the fixed plate 2444 extends out of the transfer head inner cavity 2441.

[0109] In this embodiment, a pressure sensor is also provided on the fixing plate 2444. When the motor drives the fixing plate support rod 2443 and the fixing plate 2444 to rotate inward to fix the glass slide, when the fixing plate 2444 contacts the glass slide, the pressure sensor senses a specified pressure, and the pressure sensor transmits a signal to the system, and the system controls the motor to stop working, and at the same time starts the third telescopic rod 241 and the fourth telescopic rod 242 to start working;

[0110] The glass slide transfer head 244 is provided with a suction cup 2445, which is fixedly connected to the lower end of the suction cup support rod 2442, and is connected to the pressure pump through a pipeline. When the suction cup 2445 contacts the glass slide, the pressure pump works to generate negative pressure in the suction cup 2445, thereby firmly sucking the glass slide. When the glass slide is moved to a designated position, the pressure pump works to generate positive pressure in the suction cup 2445, thereby placing the glass slide in the designated position.

[0111] A pressure sensor is provided on the suction cup 2445. When the negative pressure generated in the suction cup 2445 reaches a specified pressure, or the glass slide moving mechanism 24 moves downward and contacts the glass slide to generate a large pressure, the pressure sensor transmits a signal to the system, and the system controls the pressure pump to stop working, or controls the glass slide moving mechanism 24 to stop moving downward to prevent the glass slide from being crushed.

[0112] The suction cup 2445 works in conjunction with the fixing sheet 2444 to make the glass slide more stable during transfer and less likely to fall off.

[0113] The beneficial effects of this embodiment are as follows: through the fully automated precise sample addition and incubation and precise detection of the incubation system and the detection system, precise sample addition of automated instruments can be achieved, the sample is mixed with a sample reaction solution with an oxidizing effect in the sample incubation mechanism 14 and an incubation reaction is carried out, the unstable hemoglobin is oxidized and denatured to precipitate into insoluble Heinz bodies, which are then stained with a dye to be dyed purple, and a high-resolution electron microscope imaging system is used for image acquisition, and the Heinz bodies are analyzed and detected by AI analysis technology, thereby achieving automated detection of the experiment, improving the work efficiency of personnel, and standardizing the experimental operations, and ensuring the quality of the experimental results.

[0114] The working process of this embodiment is as follows: put the sample to be tested into the sample tube sleeve 1351 of the sample rack 135, the barcode of the sample to be tested is facing the barcode scanning port 1352, the sample rack 135 is placed in the sample injection area 133, the card slot on the sample rack 135 is engaged and slidably connected with the card strip set in the sample transfer mechanism 13, and the sample rack 135 is transferred to the sample adding area 131 through the first sensing mechanism 137 and the first driving mechanism, and the barcode scanner 1313 transmits the sample information to be tested into the incubation system. After the sample to be tested in the sample rack 135 is added to the test tube, the sample rack 135 is transferred from the sample adding area entrance 13211 to the first sample buffer area 1321 through the first sensing mechanism 137 and the first driving mechanism. When the first sample buffer area 1321 is full of the sample rack 135, the sample rack 135 is transferred from the sample buffer area entrance 13213 to the second sample buffer area 1322. Finally, the staff moves the test tube rack that has completed the test out of the sample buffer area 132.

[0115] The test tube moving mechanism 12 drives the second telescopic rod 125 to slide left and right on the first sliding mechanism 121 through the first telescopic rod 124. At the same time, the first telescopic rod 124 and the second telescopic rod 125 move up and down and forward and backward to move the test tube sleeve 126 to the test tube rack storage area 10 and align the test tube. Then, the test tube moving mechanism 12 moves the test tube to the specified position of the sample incubation mechanism 14; the test tube moving mechanism 12 can reciprocate on the first sliding mechanism 121 to realize the work of automatically and accurately taking the test tube.

[0116] After the test tube moving mechanism 12 transfers the test tube to the second test tube hole 1471 of the incubation tray 142, the second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the second sample adding hole 1472. The first sample adding mechanism 151 absorbs the sample to be tested from the first sample adding hole 1311 and adds it to the test tube to be tested through the second sample adding hole 1472. The second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the third sample adding hole 1473. The second sample adding mechanism 152 adds the reaction liquid reagent in the fourth sample adding hole 1433 to the test tube to be tested below the third sample adding hole 1473. Then, the test tube of the sample to be tested is mixed evenly with the sample and the reaction liquid reagent through the third driving mechanism, and then placed in the incubation tray 142 for constant temperature incubation at 37.0°C for 3.5 hours. After the incubation is completed, the second loading mechanism 152 adds the dye in the fourth loading hole 1433 to the test tube to be tested under the third loading hole 1473. The test tube of the sample to be tested is mixed evenly with the sample, the reaction liquid and the dye through the third driving mechanism, and then continues to incubate for 10 minutes to complete the incubation.

[0117] The detection system controls the motion system control unit 27 to move the stage 22 forward and backward and left and right. The stage 22 moves to one side of the slide storage box 25. The slide moving mechanism 24 can transfer the slide on the slide storage box 25 to the stage 22. Then, the motion system control unit 27 controls the stage 22 to move toward the sample incubation mechanism 14. The second loading mechanism 152 mixes the sample, reaction solution and dye in the test tube from the third loading hole 1473 and places the sample to be tested after incubation on the slide of the stage 22. The motion system control unit 27 controls the stage 22 to move toward the slide storage box 25. Then, the slide moving mechanism 24 can transfer the cover glass on the slide storage box 25 to the stage 22, and cover the cover glass on the slide with the sample to be tested. Then, the motion system control unit 27 controls the stage 22 to move to the bottom of the image acquisition system 21, and the light source passes through the stage 22 to irradiate the glass slide. The sample to be tested is collected by the image acquisition system 21, and the Heinz body is analyzed and detected by the AI ​​intelligent analysis system. After the first sample loading mechanism 151 and the second sample loading mechanism 152 are loaded, the first sample loading mechanism 151 and the second sample loading mechanism 152 are cleaned with the washing liquid in the washing liquid barrel 17, and the waste liquid is discharged into the waste liquid barrel 162.

[0118] Example 2

[0119] like Fig.21 As shown, based on Example 1, a detection method of a Heinz body detector is:

[0120] S1: The Heinz body detector is automatically reset, and all system components complete various preparations before the test;

[0121] S2: Put the sample to be tested into the sample tube sleeve 1351 of the sample rack 135, and engage and slide the sample rack 135 with the sample transfer mechanism 13, the first driving mechanism drives the first conveying structure 136 to move the sample rack 135, and the sample rack limit moving unit 134 controls the sample rack 135 to move between the sample injection area 133 and the sample addition area 131;

[0122] S3: Scan the barcode of the sample to be tested by the barcode scanner 1313, and transmit the information of the sample to be tested to the incubation system. The incubation system controls the test tube moving mechanism 12 to work, and the test tube moving mechanism 12 transfers the test tube from the test tube rack storage area 10 to the sample incubation mechanism 14;

[0123] S4: the second sensing mechanism of the sample incubation mechanism 14 senses that the test tube enters the incubation chamber 141, and the second driving mechanism drives the incubation disk 142 to rotate;

[0124] S5: the first sample adding mechanism 151 adds the sample into the test tube, and then the sample adding needle 155 of the first sample adding mechanism 151 is cleaned with a washing solution;

[0125] S6: the second sample adding mechanism 152 adds the reaction liquid reagent into the test tube, mixes the sample in the test tube with the reaction liquid reagent evenly and incubates them, and then uses the washing liquid to clean the sample adding needle 155 of the second sample adding mechanism 152;

[0126] S7: After all samples to be tested are added to the sample rack 135, the sample rack 135 moves to a designated position of the sample buffer area 132;

[0127] S8: After the sample in the test tube and the reaction liquid reagent are mixed evenly and incubated, the dye of the second sample adding mechanism 152 is added into the test tube, the sample to be tested, the reaction liquid reagent and the sample of the dye in the test tube are mixed evenly and incubated, and then the sample adding needle 155 of the second sample adding mechanism 152 is cleaned with a washing solution;

[0128] S9: The motion system control unit 27 of the detection system controls the stage 22 to move, and starts the slide moving mechanism 24, which places the slide on the slide storage box 25 at a designated position of the slide placement area 2231 on the third support plate 223;

[0129] S10: After the sample incubation is completed, the second driving mechanism drives the incubation plate 142 to rotate, and the second loading mechanism 152 samples the sample to be detected and adds the sample to the slide; then, the loading needle 155 of the second loading mechanism 152 is cleaned with a washing solution;

[0130] S11: After the reagent incubated in the sample test tube to be tested is sampled and tested, the test tube moving mechanism 12 transfers the test tube to the second waste storage box 161;

[0131] S12: The motion system control unit 27 of the detection system controls the stage 22 to move, and starts the slide moving mechanism 24, and the slide moving mechanism 24 transfers the cover glass on the slide storage box 25 to the sample on the slide and covers the sample;

[0132] S13: The motion system control unit 27 of the detection system controls the stage 22 to move to the bottom of the image acquisition system 21, aligns the sample slide to be detected with the image acquisition system 21, and the image acquisition system 21 acquires an image of the sample slide and uploads the acquired image to the AI ​​intelligent analysis system;

[0133] S14: Repeat steps S1-S13 multiple times according to the requirements of the test, perform multiple image acquisitions, and analyze the results;

[0134] S15: After the sample analysis is completed, the motion system control unit 27 of the detection system controls the stage 22 to move, and the slide moving mechanism 24 transfers the tested slide and cover glass on the stage 22 to the waste slide placement port 261, and transfers the slide and cover glass to the first waste disposal device 26 through the waste slide placement port 261;

[0135] S16 : the second driving mechanism drives the incubation tray 142 to rotate, and the test tube moving mechanism 12 moves the tested test tube out of the incubation chamber 141 , and transfers the tested sample test tube to the second waste storage box 161 .

[0136] The beneficial effect of adopting the above scheme is that through the fully automated precise sample addition and incubation and precise detection of the incubation system and the detection system, precise sample addition of automated instruments can be achieved. The sample is mixed with the sample reaction solution with an oxidizing effect in the sample incubation mechanism 14 and incubated to react, and the unstable hemoglobin is oxidized and denatured to precipitate into insoluble Heinz bodies, which are then stained with a dye to be dyed purple, and a high-resolution electron microscope imaging system is used for image acquisition. The Heinz bodies are analyzed and detected by AI analysis technology, thereby realizing automated detection of the experiment, improving the work efficiency of personnel, and standardizing the experimental operations, and ensuring the quality of the experimental results.

[0137] The working process of this embodiment is: turn on the power switch, the Heinz body detector automatically resets, and the temperature control system of the sample incubation mechanism 14 controls the temperature to 37°C, the temperature required for the sample reaction; the detection personnel empties the first waste disposal device 26, the second waste storage box 161 and the waste liquid barrel 162, puts a test tube rack with clean test tubes in the test tube rack storage area 10, fills the washing liquid barrel 17 with washing liquid, and installs a slide glass and a cover glass in the slide storage chamber 251 and the cover glass storage chamber 252 respectively.

[0138] Put the sample to be tested into the sample tube sleeve 1351 of the sample rack 135, with the barcode of the sample to be tested facing the barcode scanning port 1352, and place the sample rack 135 in the sample injection area 133. The card slot on the sample rack 135 is slidably connected with the card strip set in the sample transfer mechanism 13. When the sample rack 135 is put into the sample injection area 133, it touches the conveyor belt in the first conveying structure 136. When the first sensing mechanism 137 senses pressure, it transmits a signal to the incubation system, and the incubation system controls the conveyor belt to move to the left. When the sample rack 135 touches the first sensing mechanism 137, the first sensing mechanism 137 senses the pressure, transmits the signal to the system, and controls the conveyor belt to stop working. At the same time, the sample rack limit moving unit 134 is controlled to move to the right to the specified position, and the sample rack limit moving unit 134 moves upward by 0.5 mm to reduce the friction between the sample rack 135 and the conveyor belt. The sample rack limit moving unit 134 moves to the left and moves the sample rack 135 to the entrance of the sample adding area 131, and then moves backward and enters the sample adding area 131 through the entrance of the sample adding area 131.

[0139] The barcode scanner 1313 scans the barcode of the sample to be tested and transmits the information of the sample to be tested to the system. The system controls the test tube moving mechanism 12 to start working, and the first sliding mechanism 121 of the test tube moving mechanism 12 works. The first telescopic rod 124, the second telescopic rod 125 and the test tube sleeve 126 move left and right in the first sliding mechanism 121; the first telescopic rod 124, the second telescopic rod 125 and the test tube sleeve 126 move left to the test tube rack storage area 10, lock the test tube position, the first telescopic rod 124 moves upward, and the second telescopic rod 125 moves forward to move the test tube The sleeve 126 moves to above the test tube, the first telescopic rod 124 moves downward, the test tube enters the entrance and enters the test tube sleeve 126, when the sensing detection device 1267 in the test tube sleeve 126 senses the test tube, a signal is transmitted to the system, the first telescopic rod 124 is controlled to stop moving downward, and the test tube sleeve 126 is controlled to clamp the test tube, and a signal is transmitted to the system, thereby controlling the first sliding mechanism 121 of the test tube moving mechanism 12 to work in the reverse direction, driving the first telescopic rod 124, the second telescopic rod 125, the test tube sleeve 126 and the test tube to move right to the sample incubation mechanism 14.

[0140] After the samples to be tested in the sample rack 135 are added to the test tubes, the sample rack 135 is transferred from the sample adding area entrance 13211 to the first sample buffer area 1321 through the first sensing mechanism 137 and the first driving mechanism. When the first sample buffer area 1321 is full of sample racks 135, the sample rack 135 is transferred from the sample buffer area entrance 13213 to the second sample buffer area 1322. Finally, the staff moves the test tube rack that has completed the test out of the sample buffer area 132.

[0141] After the test tube moving mechanism 12 transfers the test tube to the second test tube hole 1471 of the incubation tray 142, the second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the second sample adding hole 1472. The first sample adding mechanism 151 absorbs the sample to be tested from the first sample adding hole 1311 and adds it to the test tube to be tested through the second sample adding hole 1472. The second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the third sample adding hole 1473. The second sample adding mechanism 152 adds the reaction liquid reagent in the fourth sample adding hole 1433 to the test tube to be tested below the third sample adding hole 1473. Then, the test tube of the sample to be tested is mixed evenly with the sample and the reaction liquid reagent through the third driving mechanism, and then placed in the incubation tray 142 for constant temperature incubation at 37.0°C for 3.5 hours. After the incubation is completed, the second loading mechanism 152 adds the dye in the fourth loading hole 1433 to the test tube to be tested under the third loading hole 1473. The test tube of the sample to be tested is mixed evenly with the sample, the reaction liquid and the dye through the third driving mechanism, and then continues to incubate for 10 minutes to complete the incubation.

[0142] The detection system controls the motion system control unit 27 to move the stage 22 forward, backward, left and right. The stage 22 moves to one side of the glass slide storage box 25. The third telescopic rod 241 and the fourth telescopic rod 242 work together to drive the glass slide transfer head 244 to move up and down, forward and backward, left and right. When the glass slide moving mechanism 24 controls the glass slide transfer head 244 to the top of the glass slide storage chamber 251, the third telescopic rod 241 moves downward. When the suction cup 2445 on the glass slide transfer head 244 contacts the glass slide, the first pressure sensor on the suction cup 2445 senses the pressure, and the pressure sensor transmits the signal to the system. The system controls the glass slide moving mechanism 24 to stop moving downward, and at the same time, the pressure pump connected to the suction cup 2445 is started, and the pressure pump generates The negative pressure generates negative pressure in the suction cup 2445, thereby firmly sucking the glass slide, and the third telescopic rod 241 moves upward, moving the glass slide upward. At the same time, the motor connected to the upper end of the fixed sheet support rod 2443 rotates to work, driving the fixed sheet support rod 2443 to rotate inward, thereby driving the fixed sheet 2444 to rotate inward, and further stabilizing the glass slide on the glass slide transfer head 244; the glass slide moving mechanism 24 moves the glass slide to the designated position of the glass slide placement area 2231 of the stage 22, and the third telescopic rod 241 moves downward at the same time to move the glass slide to the designated position, and the pressure pump works to generate positive pressure in the suction cup 2445, and the fixed sheet support rod 2443 drives the fixed sheet 2444 to rotate outward, thereby placing the glass slide into the designated glass slide placement area 2231. The slide moving mechanism 24 can transfer the slide on the slide storage box 25 to the stage 22, and then the control system control unit 27 controls the stage 22 to move toward the sample incubation mechanism 14, and the second loading mechanism 152 mixes the sample, reaction liquid and dye in the test tube evenly from the third loading hole 1473 and places the sample to be tested on the slide on the stage 22 after incubation. After the first loading mechanism 151 and the second loading mechanism 152 complete the loading, the loading needles 155 in the first loading mechanism 151 and the second loading mechanism 152 are cleaned by the washing liquid in the washing liquid barrel 17, and the waste liquid is discharged into the waste liquid barrel 162. The control system control unit 27 controls the stage 22 to move toward the slide storage box 25, and then the slide moving mechanism 24 can transfer the cover glass on the slide storage box 25 to the stage 22, and cover the cover glass on the slide with the sample to be tested. Then, the motion system control unit 27 controls the stage 22 to move to the bottom of the image acquisition system 21, and the light source passes through the stage 22 to illuminate the glass slide. The sample to be tested is collected data through the image acquisition system 21, and the Heinz body is analyzed and detected by the AI ​​intelligent analysis system.

[0143] For each "negative" sample, 300 pictures are collected and uploaded to the system for AI analysis, and the results are interpreted and uploaded; for each sample with "occasional" Heinz bodies, 30 pictures are collected and uploaded to the system for AI analysis, and the results are interpreted and uploaded; for each sample with a test result of "visible" or above, 10 pictures are collected and uploaded to the system for AI analysis, and the results are interpreted and uploaded;

[0144] The interpretation modes are:

[0145] The test result was "occasionally" Heinz bodies: only 1 was visible on multiple high-power visual field examinations;

[0146] The test result is "visible": 2-3 can be seen in each high-power field of view;

[0147] The test result is "+": 1 / 4 of the red blood cells in each high-power field have Heinz bodies;

[0148] The test result is "++": 1 / 3 of the red blood cells in each high-power field have Heinz bodies;

[0149] The test result is "+++": more than 1 / 2 of the red blood cells in each high-power field have Heinz bodies.

[0150] After the sample analysis is completed, the detection system motion system control unit 27 controls the stage 22 to move to the right, and the slide moving mechanism 24 works to transfer the tested slide and cover glass to the top of the waste slide placement port 261 through the fixed plate 2444 on the slide transfer head 244, and then move downward to transfer the tested slide and cover glass to the first waste disposal device 26; at the same time, the motor drives the incubation tray 142 test tube sleeve 126 and the tested sample test tube in the incubation chamber 141 to rotate and position them below the second test tube hole 1471 of the incubation chamber cover 147, and the test tube moving mechanism 12 works to move the test tube sleeve 126 into the second test tube hole 1471, and fix the tested sample test tube, further drive the tested sample test tube to move out of the incubation chamber 141 of the sample incubation mechanism 14, and move to the first test tube hole 1312, and transfer the tested sample test tube to the second waste storage box 161.

[0151] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0152] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0153] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0154] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0155] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, mechanisms, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, mechanisms, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0156] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A Heinz body detector, characterized in that: Including incubation system and detection system, The incubation system comprises a test tube rack storage area (10), a test tube moving mechanism (12), a sample transfer mechanism (13), a sample incubation mechanism (14) and a sample loading mechanism (15); the incubation system is arranged in an incubation box (1); the test tube rack storage area (10) is arranged on the left side of the incubation box (1); the test tube rack storage area (10) is provided with a plurality of test tube rack placement positions (101); the test tube moving mechanism (12) is slidably arranged at the rear end of the test tube rack storage area (10); the sample transfer mechanism (13) is arranged at the front end of the test tube rack storage area (10); the sample incubation mechanism (14) and the sample loading mechanism (15) are arranged on the right side of the incubation box (1); and the sample loading mechanism (15) is arranged on the sample incubation mechanism (14); The detection system comprises an image acquisition system (21), a stage (22), a light source control unit (23), a slide moving mechanism (24), a slide storage box (25), a motion system control unit (27) and an AI intelligent analysis system. The detection system is arranged in the detection box (2), the AI ​​intelligent analysis system is electrically connected to the image acquisition system (21), the lens of the image acquisition system (21) faces the stage (22), and the stage (22) is moved by the motion system control unit (27). 7) is movably arranged in the detection box (2) frontward and rearward and leftward and rightward, the light source control unit (23) is arranged below the loading platform (22), and the light source can pass through the loading platform (22) to illuminate the glass slide, the glass slide storage box (25) is arranged on one side of the glass slide moving mechanism (24), the glass slide moving mechanism (24) is slidably arranged on one side of the detection box (2), and the glass slide transfer head (244) of the glass slide moving mechanism (24) acts on the glass slide on the glass slide storage box (25).

2. A Heinz body detector according to claim 1, characterized in that: The test tube moving mechanism (12) comprises a first sliding mechanism (121), a first telescopic rod (124), a second telescopic rod (125) and a test tube sleeve (126); the lower end of the first telescopic rod (124) is fixedly arranged on the first sliding mechanism (121); the upper end of the first telescopic rod (124) is fixedly connected to one end of the second telescopic rod (125); and the other end of the second telescopic rod (125) is fixedly connected to the test tube sleeve (126); The sample transfer mechanism (13) comprises a sample injection area (133), a sample buffer area (132), a sample adding area (131), a sample rack limit moving unit (134), a sample rack (135), a first conveying structure (136), a first sensing mechanism (137) and a first driving mechanism. The sample injection area (133) is arranged on the right side of the sample transfer mechanism (13), the sample buffer area (132) is arranged on the left side of the sample transfer mechanism (13), and the sample buffer area (131) is arranged on the left side of the sample transfer mechanism (13). The sample buffer (132) comprises a first sample buffer area (1321) and a second sample buffer area (1322), the sample feed area (133) is connected to the sample adding area (131) through a sample adding area inlet (13211), the first sample buffer area (1321) is connected to the second sample buffer area (1322) through a sample buffer area inlet (13213), and the bottoms of the sample feed area (133), the sample buffer area (132) and the sample adding area (131) are all provided with the first transmission The first transmission structure (136) is provided with the first sensing mechanism (137) and the first driving mechanism at the bottom thereof; the first sensing mechanism (137) is provided at the entrance (13211) of the sample adding area and the entrance (13213) of the sample buffer area; the sample rack (135) is slidably connected with the sample adding area (133), the sample buffer area (132) and the sample rack limit moving unit (134); the sample rack limit moving unit (135) is connected with the sample adding area (133), the sample buffer area (132) and the sample rack limit moving unit (134); The element (134) is composed of a PLC controller, the top of the sample injection area (133) is provided with a first sample injection hole (1311) and a first test tube hole (1312), a barcode scanner (1313) is arranged on the side of the sample injection area (133) and at a position corresponding to the first sample injection hole (1311), a plurality of sample tube sleeves (1351) are arranged on the top of the sample rack (135), and a barcode scanning port (1352) is arranged on the side of the plurality of sample tube sleeves (1351).

3. A Heinz body detector according to claim 1, characterized in that: The sample incubation mechanism (14) comprises an incubation chamber (141), an incubation tray (142), a reagent storage chamber (143), a sample mixing mechanism (144), a second sensing mechanism, a second driving mechanism, an incubation chamber cover (147), a third driving mechanism and a third sensing mechanism. The incubation tray (142) is arranged in the incubation chamber (141). A through hole is opened in the center of the incubation tray (142). The reagent storage chamber (143) passes through the through hole and is placed in the through hole. A reagent position (1431) is arranged in the reagent storage chamber (143). The incubation chamber (141) is provided with a second sensing mechanism and a second driving mechanism. The incubation chamber cover (147) is provided above the incubation chamber (141), and a second test tube hole (1471), a second sample loading hole (1472) and a third sample loading hole (1473) are provided on the incubation chamber cover (147). A reagent storage chamber cover (1432) is provided above the reagent storage chamber (143), and a fourth sample loading hole (1433) is provided on the reagent storage chamber cover (1432). The sample mixing mechanism (144) is correspondingly provided below the third sample loading hole (1473), and the third driving mechanism and the third sensing mechanism are provided below the sample mixing mechanism (144).

4. A Heinz body detector according to claim 3, characterized in that: The sample loading mechanism (15) comprises a first sample loading mechanism (151) and a second sample loading mechanism (152). The sample loading mechanism (15) comprises a lifting rod (153), a rotating arm (154), a motor and a sample loading needle (155). A gear is arranged at the lower end of the lifting rod (153). The gear at the lower end of the lifting rod (153) is connected to the gear of the motor. The upper end of the lifting rod (153) is fixedly connected to one end of the rotating arm (154). The other end of the rotating arm (154) is fixedly connected to the sample loading needle (155). The upper end of the sample loading needle (155) is connected to a washing liquid pipe and a pressure pipe. The ends of the washing liquid pipes are connected to sensing devices.

5. The Heinz body detector according to claim 1, characterized in that: The incubation box (1) is arranged on one side of the detection box (2); an incubation box cover (11) is arranged on the top of the incubation box (1); the incubation box cover (11) is hinged to one side of the incubation box (1); a detection box cover is arranged on the top of the detection box (2); the detection box cover is hinged to one side of the detection box (2); and one end of the image acquisition system (21) of the detection system is fixedly arranged on the detection box cover.

6. A Heinz body detector according to claim 2, characterized in that: The incubation system further comprises a second waste disposal device (16) and a washing liquid bucket (17); the incubation box (1) is divided into an upper and lower layer; the test tube rack storage area (10), the test tube moving mechanism (12), the sample transfer mechanism (13), the sample incubation mechanism (14) and the sample loading mechanism (15) are arranged on the upper layer of the incubation box (1); and the second waste disposal device (16) and the washing liquid bucket (17) are arranged on the lower layer of the incubation box (1); The second waste disposal device (16) comprises a second waste storage box (161) and a waste liquid bucket (162), the lower part of the first test tube hole (1312) is connected to the second waste storage box (161), and the bottom of the sample adding mechanism (15) is connected to the waste liquid bucket (162) and the washing liquid bucket (17); The detection system further comprises a first waste disposal device (26); the detection box (2) is divided into an upper and lower layer; the image acquisition system (21), the stage (22), the glass slide moving mechanism (24) and the glass slide storage box (25) are arranged on the upper layer of the detection box (2); the light source control unit (23), the first waste disposal device (26) and the motion system control unit (27) are arranged on the lower layer of the detection box (2); the glass slide storage box (25) is arranged on the first waste disposal device (26); a waste glass slide placement opening (261) is provided at the top of one end of the first waste disposal device (26); and the waste glass slide placement opening (261) is located behind the glass slide storage box (25).

7. The Heinz body detector according to claim 1, characterized in that: The image acquisition system (21) comprises a lens (211), an image acquisition device (212), a focusing ring (213), a fixing plate (214), a fifth telescopic rod (215), and a second transmission structure (216); the top of the fifth telescopic rod (215) is fixedly arranged on the top of the detection box (2); the bottom end of the fifth telescopic rod (215) is fixedly connected to the fixing plate (214); the bottom of the fixing plate (214) is provided with the second transmission structure (216); the second transmission structure (216) acts on the focusing ring (213); the lens (211) is rotatably connected to the image acquisition device (212) through the focusing ring (213); the upper end of the image acquisition device (212) is fixedly connected to the fixing plate (214); and the lower end of the image acquisition device (212) is threadedly connected to the focusing ring (213).

8. The Heinz body detector according to claim 1, characterized in that: The stage (22) comprises a first support plate (221), a second support plate (222), and a third support plate (223); the first support plate (221) is arranged above the light source control unit (23) and the first waste disposal device (26); the second support plate (222) is arranged on the first support plate (221) for sliding left and right; the third support plate (223) is arranged on the second support plate (222) for sliding front and back; the bottom plate of the first support plate (221) is provided with a light source entrance (2211); the bottom plate of the second support plate (222) is provided with a light transmission area (2221); the third support plate (223) is provided with a plurality of glass slide placement areas (2231); the light source of the light source control unit (23) can be projected onto the glass slide placement area (2231) through the light source entrance (2211) and the light transmission area (2221); The motion system control unit (27) comprises a first motor (271), a first screw (272), a first screw fixing plate (273), a second motor (274), a second screw (275) and a second screw fixing plate (276), the first support plate (221) is provided with a first screw fixing plate (273) at both ends, the first screw (272) is connected to the two first screw fixing plates (273) at both ends, one end of the first screw (272) passes through a screw connecting hole provided on the second support plate (222), and one end of the first screw (272) is connected to the second support plate (221). The first motor (271) is provided, and the second support plate (222) is slidably arranged on the first support plate (221) by the first screw rod (272) to the left and right. The second support plate (222) is provided with the second screw rod fixing plate (276) and the second motor (274) at a position away from the light-transmitting area (2221). The second screw rod fixing plate (276) is also provided with a nut. The output end of the second motor (274) is connected to the nut. The second screw rod (275) is provided at the rear of the third support plate (223), and one end of the second screw rod (275) is screwed to the nut.

9. The Heinz body detector according to claim 1, characterized in that: The glass slide storage box (25) is provided with a plurality of glass slide storage chambers (251) and a plurality of cover glass storage chambers (252); the glass slide storage chambers (251) are larger than the cover glass storage chambers (252); the glass slide storage chambers (251) and the cover glass storage chambers (252) are arranged in a convex hollow shape; The glass slide moving mechanism (24) comprises a third telescopic rod (241), a fourth telescopic rod (242), a second sliding mechanism (243) and a glass slide transfer head (244); one end of the third telescopic rod (241) is fixedly connected to the top of the fourth telescopic rod (242), and the other end is connected to the glass slide transfer head (244); the fourth telescopic rod (242) is a telescopic structure, and the bottom end of the fourth telescopic rod (242) is slidably arranged on the second sliding mechanism (243).

10. A method for detecting Heinz bodies, characterized in that: A Heinz body detector according to any one of claims 1 to 9, wherein the detection method is: S1: The Heinz body detector is automatically reset, and all system components complete various preparations before the test; S2: placing the sample to be tested into the sample tube sleeve (1351) of the sample rack (135), and engaging and slidingly connecting the sample rack (135) with the sample transfer mechanism (13), the first driving mechanism drives the first conveying structure (136) to move the sample rack (135), and the sample rack limit moving unit (134) controls the sample rack (135) to move between the sample injection area (133) and the sample addition area (131); S3: Scanning the barcode of the sample to be tested by the barcode scanner (1313), transmitting the information of the sample to be tested to the incubation system, and the incubation system controls the test tube moving mechanism (12) to work, and the test tube moving mechanism (12) transfers the test tube from the test tube rack storage area (10) to the sample incubation mechanism (14); S4: the second sensing mechanism of the sample incubation mechanism (14) senses that the test tube has entered the incubation chamber (141), and the second driving mechanism drives the incubation plate (142) to rotate; S5: the first sample adding mechanism (151) adds the sample into the test tube, and then uses a washing liquid to clean the sample adding needle (155) of the first sample adding mechanism (151); S6: The second sample adding mechanism (152) adds the reaction liquid reagent into the test tube, mixes the sample in the test tube with the reaction liquid reagent evenly and incubates, and then uses a washing solution to clean the sample adding needle (155) of the second sample adding mechanism (152); S7: After all samples to be tested on the sample rack (135) are added, the sample rack (135) moves to a designated position of the sample buffer area (132); S8: After the sample in the test tube and the reaction liquid reagent are mixed evenly and incubated, the second sample adding mechanism (152) adds the dye into the test tube, the sample to be tested, the reaction liquid reagent and the sample of the dye in the test tube are mixed evenly and incubated, and then the sample adding needle (155) of the second sample adding mechanism (152) is cleaned with a washing solution; S9: The motion system control unit (27) of the detection system controls the movement of the stage (22) and starts the glass slide moving mechanism (24), and the glass slide moving mechanism (24) places the glass slide on the glass slide storage box (25) at a designated position of the glass slide placement area (2231) on the third support plate (223); S10: After the sample incubation is completed, the second driving mechanism drives the incubation plate (142) to rotate, and the second loading mechanism (152) samples the sample to be tested and adds the sample to the slide; then, the loading needle (155) of the second loading mechanism (152) is cleaned with a washing solution; S11: After the reagent incubated in the sample test tube to be tested is sampled and tested, the test tube moving mechanism (12) transfers the test tube to the second waste storage box (161); S12: The motion system control unit (27) of the detection system controls the stage (22) to move, and starts the slide moving mechanism (24), and the slide moving mechanism (24) transfers the cover glass on the slide storage box (25) to the sample on the slide, and covers the sample; S13: The motion system control unit (27) of the detection system controls the stage (22) to move to the bottom of the image acquisition system (21), aligns the sample slide to be detected with the image acquisition system (21), and the image acquisition system (21) acquires an image of the sample slide and uploads the acquired image to the AI ​​intelligent analysis system; S14: Repeat steps S1-S13 multiple times according to the requirements of the test, perform multiple image acquisitions, and analyze the results; S15: After the sample analysis is completed, the motion system control unit (27) of the detection system controls the stage (22) to move, and the glass slide moving mechanism (24) transfers the tested glass slide and cover glass on the stage (22) to the waste glass slide placement port (261), and transfers the glass slide and cover glass to the first waste disposal device (26) through the waste glass slide placement port (261); S16: The second driving mechanism drives the incubation plate (142) to rotate, and the test tube moving mechanism (12) moves the tested test tube out of the incubation chamber (141), and transfers the tested sample test tube to the second waste storage box (161).

Images