A method for purifying alkaloids from oats and its application in the preparation of an anti-allergy and soothing composition

Abstract

The present invention belongs to the technical field of daily chemical products, and particularly relates to a method for purifying alkaloids from oats and their application in preparing an anti-allergic and soothing composition. The method for purifying alkaloids from oats comprises the following steps: (1) crushing oats to obtain oat powder; (2) mixing oats and water in a weight ratio of 1:(10-15), putting them into a microwave reactor for microwave-assisted extraction to obtain a mixture; (3) performing enzymatic hydrolysis to obtain an enzymatic hydrolysate; (4) adding a mixed bacterium to the enzymatic hydrolysate for fermentation and sterilization to obtain a fermentation broth; (5) centrifuging the fermentation broth, subjecting the solid obtained by centrifugation to subcritical extraction, and then recovering the solvent to obtain an extract containing oat alkaloids. The method of the present invention has a high extraction rate of oat alkaloids and a good synergistic effect with sophora flavescens extract.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily chemical products, and particularly relates to a method for purifying alkaloids from oats and their application in the preparation of an anti-allergic and soothing composition. Background Art

[0002] Oat alkaloids are a class of natural compounds mainly found in oats. These compounds belong to polyphenols and have antioxidant, anti-inflammatory, and potential cardiovascular protective effects. Due to their significant anti-inflammatory and antioxidant properties, oat alkaloids have shown good effects in anti-allergic and soothing aspects. Because of their excellent anti-allergic and soothing properties, oat alkaloids are widely used in various skin care products, including creams, lotions, facial cleansers, etc., especially products designed for sensitive skin.

[0003] Sophora flavescens, also known as Sophora flavescens root, is a traditional Chinese medicine. Its extract is widely used in skin care products due to its various bioactive components, especially in soothing products. Components such as matrine and oxymatrine have significant anti-inflammatory properties and can inhibit the release of inflammatory reaction mediators, reducing symptoms such as skin redness and itching. Matrine and oat alkaloids are two components often used in combination during the research and development of cosmetics. However, it was found during the research that adding commercially available oat alkaloids and Sophora flavescens extract to oat alkaloids simultaneously would result in poor storage stability of the product. During the process of extracting oat alkaloids, in addition to the target components, other substances may also be extracted simultaneously. These impurities may have adverse reactions with matrine in the Sophora flavescens extract, which may affect the storage effect of the final product.

[0004] Therefore, there is an urgent need for a method for purifying alkaloids from oats so that the extracted oat alkaloids can be safely added and have a better use effect in anti-allergic and soothing products in combination with Sophora flavescens extract. Summary of the Invention

[0005] The purpose of the present invention is to provide a method for purifying alkaloids from oats and their application in the preparation of an anti-allergic and soothing composition.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] A method for purifying alkaloids from oats, comprising the following steps:

[0008] (1) Crushing oats to obtain oat powder;

[0009] (2) Mixing oats and water in a weight ratio of 1:(10 - 15), putting them into a microwave reactor for microwave-assisted extraction to obtain a mixture;

[0010] (3) Add neutral protease to the mixture, enzymatically hydrolyze at 45°C - 48°C for 50 - 60 min; inactivate the enzyme, adjust the pH value to 8 - 8.5, add alkaline protease, enzymatically hydrolyze at 45°C - 48°C for 30 - 40 min; adjust the pH value to 6.2 - 6.5, inactivate the enzyme, continue to add ficin, enzymatically hydrolyze at 45°C - 48°C for 20 - 30 min; inactivate the enzyme to obtain the enzymolysis solution;

[0011] (4) Add mixed bacteria to the enzymolysis solution for fermentation, sterilize to obtain the fermentation broth;

[0012] (5) Centrifuge the fermentation broth, perform subcritical extraction on the solid obtained by centrifugation, and then recover the solvent to obtain an extract containing avenanthramides.

[0013] Further, in the step (3), the dosage of the neutral protease is 700 - 800 u / mL of the mixture; the dosage of the alkaline protease is 900 - 1000 u / mL of the mixture; the dosage of the ficin is 500 - 600 u / mL of the mixture.

[0014] The present invention can improve the synergistic soothing effect of the extract containing avenanthramides and the sophora flavescens extract by performing gradient fermentation on oats using specific enzymes. The analysis shows that the content of avenanthramides in the avenanthramide extract prepared by gradient fermentation is higher, and there are fewer impurities that have side effects on the sophora flavescens extract, which is more conducive to the synergistic effect of the two components.

[0015] Further, the mixed bacteria in the step (4) include saccharomyces cerevisiae, lactobacillus gasseri, and lactobacillus helveticus.

[0016] Further, the dosage of the saccharomyces cerevisiae is 10 8 -10 9 CFU / mL of the enzymolysis solution; the dosage of the lactobacillus gasseri is 10 8 -10 9 CFU / mL of the enzymolysis solution; the dosage of the lactobacillus helveticus is 10 6 -10 7 CFU / mL of the enzymolysis solution.

[0017] The present invention can improve the storage stability of the mixture of the avenanthramide extract and matrine by fermenting oats using specific bacteria. The analysis shows that certain metabolites generated during the fermentation process by specific bacteria may form complexes with avenanthramides and matrine, providing physical barriers or chemical protection, and at the same time can decompose the impurities with side effects generated by enzymatic hydrolysis, reducing the impact on matrine.

[0018] Further, the fermentation conditions in the step (4) are fermentation at 35 - 38°C for 10 - 14 hours.

[0019] Further, in the step (1), the oats are crushed to less than 80 mesh.

[0020] Further, in the step (2), the conditions for microwave-assisted extraction are: microwave power 200 - 250 W, extraction time 1 - 2 hours.

[0021] Further, in the step (5), the conditions for subcritical extraction are: the extractant is propane, the extraction temperature is 50 - 70 °C, the pressure is 3 - 4 MPa, the extractant contains 3 - 4% by volume of entrainer, and the entrainer is ethanol.

[0022] The present invention also provides the application of the method for purifying alkaloids from oats in the preparation of an anti-allergic and soothing composition.

[0023] The oats used in the present invention are Zhangbei naked oats from Zhangbei County, Zhangjiakou, Hebei. The naked oat varieties include but are not limited to: Zhangbei Dayoumai, Fengning Datan, North China No. 2, Baxuan No. 1, Baxuan No. 2, Baxuan No. 3, Baxuan No. 4, Jizhangyou No. 1, Jizhangyou No. 2, Jizhangyou No. 3, Jizhangyou No. 4, Jizhangyou No. 5, Jizhangyou No. 6, Jizhangyou No. 11, Jizhangyou No. 12, Jizhangyou No. 14, Huazao No. 2, Huawan No. 6, Huazhong 21, Yuanza No. 1, Yuanza No. 2, Bayou No. 1, Bayou No. 3, Bayou No. 5, Bayou No. 6, Bayou No. 8, Bayou No. 9, Pin No. 2, North China No. 1, etc.

[0024] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0025] 1. The oat alkaloids extracted by the present invention are a class of bioactive compounds extracted from oats, and have excellent anti-allergic and soothing effects in skin care products. The oat alkaloids of the present invention can help reduce the sensitive reaction of the skin to external stimuli, reduce the release of histamine by stabilizing mast cells in the skin, thereby reducing the incidence and severity of allergic reactions. It is particularly effective in relieving skin inflammation, itching and redness. They help repair the damaged skin barrier, promote the improvement of the skin's self-repair ability, making the skin healthier and smoother. Widely used in skin care products for sensitive skin, post-sun repair products and products for treating mild skin inflammation, oat alkaloids, as a natural and highly effective skin care ingredient, are outstanding in providing anti-allergic and soothing effects and are suitable for maintaining and restoring skin health.

[0026] 2. The present invention can improve the synergistic soothing effect of the extract containing oat alkaloids and the extract of Sophora flavescens by performing gradient fermentation of oats with specific enzymes.

[0027] 3. The present invention can improve the storage stability of the mixture of oat alkaloid extract and matrine by fermenting oats with specific bacteria. Detailed implementation manners

[0028] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Apparently, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without any creative efforts shall fall within the protection scope of the present invention.

[0029] The raw materials used in the following embodiments of the present invention are all commercially available products:

[0030] Candida utilis, strain number: CICC 1769, purchased from China Center for Industrial Culture Collection.

[0031] Lactobacillus plantarum, strain number: CICC 24936, purchased from China Center for Industrial Culture Collection.

[0032] Bifidobacterium adolescentis, strain number: CICC 6070, purchased from China Center for Industrial Culture Collection.

[0033] Saccharomyces cerevisiae, strain number: CICC 1288, purchased from China Center for Industrial Culture Collection.

[0034] Lactobacillus gasseri, strain number: SHBCC D25201, purchased from Shanghai Collection of Microorganisms Co., Ltd.

[0035] Lactobacillus helveticus, strain number: CICC 6273, purchased from China Center for Industrial Culture Collection.

[0036] Pectinase, enzyme activity 50000u / g. Shanghai Yuanye Bio-Technology Co., Ltd.

[0037] Bromelain, enzyme activity 1200u / mg. Shanghai Yuanye Bio-Technology Co., Ltd.

[0038] Neutral protease, enzyme activity 100u / mg. Shanghai Yuanye Bio-Technology Co., Ltd.

[0039] Papain, enzyme activity 800u / mg. Shanghai Yuanye Bio-Technology Co., Ltd.

[0040] Ficin, enzyme activity 100u / mg. Shanghai Yuanye Bio-Technology Co., Ltd.

[0041] Alkaline protease, enzyme activity 200u / mg. Shanghai Yuanye Bio-Technology Co., Ltd.

[0042] Oats, Zhangbei naked oats, Zhangbei County, Zhangjiakou, Hebei, variety Zhangbei Dayoumai.

[0043] Example 1

[0044] This embodiment provides a method for purifying alkaloids from oats, comprising the following steps:

[0045] (1) Grinding oats to less than 80 mesh to obtain oat powder;

[0046] (2) mixing oats and water in a weight ratio of 1:12, and placing the mixture in a microwave reactor for microwave-assisted extraction. The conditions for the microwave-assisted extraction are: microwave power of 220 W, extraction time of 1.5 hours, to obtain a mixture;

[0047] (3) Add neutral protease to the mixture, perform enzymolysis at 46°C for 55 minutes; inactivate the enzyme, adjust the pH value to 8.2, add alkaline protease, perform enzymolysis at 46°C for 35 minutes; adjust the pH value to 6.3, inactivate the enzyme, continue to add fig protease, perform enzymolysis at 46°C for 25 minutes; inactivate the enzyme to obtain enzymolysis solution; the amount of the neutral protease is 750u / mL of the mixture; the amount of the alkaline protease is 950u / mL of the mixture; the amount of the fig protease is 550u / mL of the mixture.

[0048] (4) adding mixed bacteria to the enzymatic hydrolysate for fermentation at 36° C. for 12 hours, sterilizing, and obtaining a fermentation solution; the mixed bacteria include saccharomyces cerevisiae, Lactobacillus gasseri, and Lactobacillus helveticus; the amount of saccharomyces cerevisiae is 10 8 CFU / mL enzymatic solution; the dosage of Lactobacillus gasseri is 10 9 CFU / mL enzymatic solution; the dosage of Lactobacillus helveticus is 10 7 CFU / mL enzymatic solution.

[0049] (5) The fermentation liquid is centrifuged, and the solid obtained by centrifugation is subjected to subcritical extraction. The conditions of the subcritical extraction are: the extractant is propane, the extraction temperature is 60° C., the pressure is 3.5 MPa, the extractant contains an entrainer with a volume fraction of 3.5%, and the entrainer is ethanol. Then, the solvent is recovered by vacuum rotary evaporation to obtain an extract containing oat alkaloids.

[0050] Example 2

[0051] This embodiment provides a method for purifying alkaloids from oats, comprising the following steps:

[0052] (1) Grinding oats to less than 80 mesh to obtain oat powder;

[0053] (2) mixing oats and water in a weight ratio of 1:10, placing the mixture in a microwave reactor for microwave-assisted extraction, wherein the microwave-assisted extraction conditions are: microwave power of 200 W, extraction time of 2 hours, to obtain a mixture;

[0054] (3) Add neutral protease to the mixture, enzymatically hydrolyze at 45 °C for 60 min; inactivate the enzyme, adjust the pH value to 8, add alkaline protease, enzymatically hydrolyze at 48 °C for 30 min; adjust the pH value to 6.5, inactivate the enzyme, continue to add ficin, enzymatically hydrolyze at 45 °C for 30 min; inactivate the enzyme to obtain an enzymatic hydrolysate; the dosage of the neutral protease is 800 u / mL of the mixture; the dosage of the alkaline protease is 900 u / mL of the mixture; the dosage of the ficin is 600 u / mL of the mixture.

[0055] (4) Add mixed bacteria to the enzymatic hydrolysate for fermentation, and the fermentation conditions are 35 °C for 14 hours, sterilize to obtain a fermentation broth; the mixed bacteria include Saccharomyces cerevisiae, Lactobacillus gasseri and Lactobacillus helveticus; the dosage of the Saccharomyces cerevisiae is 10 9 CFU / mL of the enzymatic hydrolysate; the dosage of the Lactobacillus gasseri is 10 9 CFU / mL of the enzymatic hydrolysate; the dosage of the Lactobacillus helveticus is 10 6 CFU / mL of the enzymatic hydrolysate.

[0056] (5) Centrifuge the fermentation broth, and subject the solid obtained by centrifugation to subcritical extraction. The conditions of the subcritical extraction are as follows: the extractant is propane, the extraction temperature is 70 °C, the pressure is 3 MPa, the extractant contains 4% (volume fraction) of an entrainer, and the entrainer is ethanol. Then, recover the solvent by rotary evaporation under reduced pressure to obtain an extract containing avenanthramides.

[0057] Comparative Example 1

[0058] The difference between this comparative example and Example 1 is: avenanthramides prepared in Example 1 of Chinese Patent 201611168303.X.

[0059] Comparative Example 2

[0060] The difference between this comparative example and Example 1 is: without microwave-assisted extraction.

[0061] A method for purifying avenanthramides from oats, comprising the following steps:

[0062] (1) Grind oats to less than 80 mesh to obtain oat powder;

[0063] (2) Mix oats and water at a weight ratio of 1:10 to obtain a mixture;

[0064] (3) Add neutral protease to the mixture, enzymatically hydrolyze at 45 °C for 60 min; inactivate the enzyme, adjust the pH value to 8, add alkaline protease, and enzymatically hydrolyze at 48 °C for 30 min; adjust the pH value to 6.5, inactivate the enzyme, and continue to add ficin, enzymatically hydrolyze at 45 °C for 30 min; inactivate the enzyme to obtain an enzymatic hydrolysate; the dosage of the neutral protease is 800 u / mL of the mixture; the dosage of the alkaline protease is 900 u / mL of the mixture; the dosage of the ficin is 600 u / mL of the mixture.

[0065] (4) Add mixed bacteria to the enzymatic hydrolysate for fermentation. The fermentation conditions are: ferment at 35 °C for 14 hours, and sterilize to obtain a fermentation broth; the mixed bacteria include Saccharomyces cerevisiae, Lactobacillus gasseri, and Lactobacillus helveticus; the dosage of Saccharomyces cerevisiae is 10 9 CFU / mL of the enzymatic hydrolysate; the dosage of Lactobacillus gasseri is 10 9 CFU / mL of the enzymatic hydrolysate; the dosage of Lactobacillus helveticus is 10 6 CFU / mL of the enzymatic hydrolysate.

[0066] (5) Centrifuge the fermentation broth, and perform subcritical extraction on the solid obtained by centrifugation. The conditions for the subcritical extraction are: the extractant is propane, the extraction temperature is 70 °C, the pressure is 3 MPa, the extractant contains 4% (by volume) of an entrainer, and the entrainer is ethanol. Then, recover the solvent to obtain an extract containing avenanthramides.

[0067] Comparative Example 3

[0068] The difference between this comparative example and Example 1 is that enzymatic hydrolysis is not performed.

[0069] A method for purifying avenanthramides from oats, comprising the following steps:

[0070] (1) Grind oats to less than 80 mesh to obtain oat powder;

[0071] (2) Mix oats and water at a weight ratio of 1:12, place them in a microwave reactor for microwave-assisted extraction. The conditions for microwave-assisted extraction are: microwave power 220 W, extraction time 1.5 hours, to obtain a mixture;

[0072] (3) Add mixed bacteria to the mixture for fermentation. The fermentation conditions are: ferment at 36 °C for 12 hours, and sterilize to obtain a fermentation broth; the mixed bacteria include Saccharomyces cerevisiae, Lactobacillus gasseri, and Lactobacillus helveticus; the dosage of Saccharomyces cerevisiae is 10 8 CFU / mL of the enzymatic hydrolysate; the dosage of Lactobacillus gasseri is 10 9 CFU / mL of the enzymatic hydrolysate; the dosage of Lactobacillus helveticus is 10 7 CFU / mL of the enzymatic hydrolysate.

[0073] (4) Centrifuge the fermentation broth, and subject the solid obtained from the centrifugation to subcritical extraction. The conditions for the subcritical extraction are as follows: the extraction agent is propane, the extraction temperature is 60 °C, the pressure is 3.5 MPa, the extraction agent contains an entrainer with a volume fraction of 3.5%, the entrainer is ethanol, and then the solvent is recovered to obtain an extract containing avenanthramides.

[0074] Comparative Example 4

[0075] The difference between this comparative example and Example 1 is that different enzymes are used for enzymatic hydrolysis.

[0076] A method for purifying avenanthramides from oats, comprising the following steps:

[0077] Specifically: Add bromelain to the mixture, and carry out enzymatic hydrolysis at 45 °C for 60 min; inactivate the enzyme, add papain, and carry out enzymatic hydrolysis at 48 °C for 30 min; adjust the pH value to 6.5, inactivate the enzyme, and continue to add pectinase, and carry out enzymatic hydrolysis at 45 °C for 30 min; inactivate the enzyme to obtain an enzymatic hydrolysate; the dosage of bromelain is 800 u / mL of the mixture; the dosage of papain is 900 u / mL of the mixture; the dosage of pectinase is 600 u / mL of the mixture.

[0078] Comparative Example 5

[0079] The difference between this comparative example and Example 1 is that fermentation is not carried out.

[0080] A method for purifying avenanthramides from oats, comprising the following steps:

[0081] (1) Crush oats to less than 80 mesh to obtain oat powder;

[0082] (2) Mix oats and water at a weight ratio of 1:10, put them into a microwave reactor for microwave-assisted extraction. The conditions for the microwave-assisted extraction are: microwave power 200 W, extraction time 2 hours, to obtain a mixture;

[0083] (3) Add neutral protease to the mixture, and carry out enzymatic hydrolysis at 45 °C for 60 min; inactivate the enzyme, adjust the pH value to 8, add alkaline protease, and carry out enzymatic hydrolysis at 48 °C for 30 min; adjust the pH value to 6.5, inactivate the enzyme, and continue to add ficin, and carry out enzymatic hydrolysis at 45 °C for 30 min; inactivate the enzyme to obtain an enzymatic hydrolysate; the dosage of neutral protease is 800 u / mL of the mixture; the dosage of alkaline protease is 900 u / mL of the mixture; the dosage of ficin is 600 u / mL of the mixture.

[0084] (4) Centrifuge the enzymolysis solution, and subject the solid obtained by centrifugation to subcritical extraction. The conditions for the subcritical extraction are as follows: the extraction solvent is propane, the extraction temperature is 70 °C, the pressure is 3 MPa, the extraction solvent contains an entrainer with a volume fraction of 4%, and the entrainer is ethanol. Then, recover the solvent to obtain an extract containing avenanthramides.

[0085] Comparative Example 6

[0086] The difference between this comparative example and Example 1 is that different bacteria are used for fermentation.

[0087] Specifically: the mixed bacteria include Candida utilis, Lactobacillus plantarum, and Bifidobacterium adolescentis; the dosage of Candida utilis is 10 9 CFU / mL of the enzymolysis solution; the dosage of Lactobacillus plantarum is 10 9 CFU / mL of the enzymolysis solution; the dosage of Bifidobacterium adolescentis is 10 6 CFU / mL of the enzymolysis solution.

[0088] Performance Test

[0089] 1. Extraction rate of avenanthramides: The extraction rate of avenanthramides in the avenanthramide extract prepared in Examples 1-2 and Comparative Examples 1-6 = weight of avenanthramides in the extract containing avenanthramides / weight of oats * 100%. The results are shown in Table 1.

[0090] The test method refers to the literature: Ren Yi, Ren Guixing. Study on the extraction of avenanthramides and their antioxidant activities [J]. Transactions of the Chinese Society of Agricultural Engineering, 2008, 24(5): 265-269.

[0091] Table 1 Test results of extraction rate

[0092] Test Extraction rate of avenanthramides % Example 1 5.31 Example 2 5.26 Comparative Example 1 4.63 Comparative Example 2 5.04 Comparative Example 3 4.82 Comparative Example 4 4.96 Comparative Example 5 4.75 Comparative Example 6 4.91

[0093] 2. Prepare an anti-allergic and soothing composition using the extracts containing avenanthramides prepared in Examples 1-2 and Comparative Examples 1-6, including the following components in mass percentages: 2% Sophora flavescens extract (high-purity matrine 98%, Shaanxi Pinhong Biotechnology Co., Ltd.), 3% extract containing avenanthramides, 50% butanediol, and the balance is water.

[0094] (1). Soothing test:

[0095] Hyaluronic acid is widely present in the skin and other tissues. The relationship between hyaluronic acid and the skin is very close. Macromolecular hyaluronic acid can inhibit the inflammatory response, thus alleviating the discomfort caused by skin inflammation. Macromolecular hyaluronic acid will be degraded under the action of hyaluronidase, resulting in a decrease in the content of macromolecular hyaluronic acid. The degradation product N-acetylglucosamine is produced by decomposition. Measuring N-acetylglucosamine in the reaction system can indirectly reflect the activity of hyaluronidase. The anti-allergic activity of the sample is evaluated by an in vitro experiment to inhibit the activity of hyaluronidase. Inhibiting the activity of hyaluronidase can reduce the degradation of hyaluronic acid and alleviate skin sensitivity.

[0096] Detection method: The anti-allergic and soothing composition was mixed with the hyaluronidase solution respectively. The concentration of the composition in the solution was 7w%. After shaking and mixing, it was incubated at 37°C for 10 min. The hyaluronate solution was added and incubated at 37°C for 45 min. The BSA solution was added and incubated for another 10 min. The OD600nm value was measured, and the hyaluronidase inhibition rate was calculated. The results are shown in Table 2.

[0097] Table 2 Soothing test results

[0098] Test Inhibition rate Example 1 99.2 Example 2 98.4 Comparative Example 1 85.8 Comparative Example 2 94.0 Comparative Example 3 92.4 Comparative Example 4 93.5 Comparative Example 5 90.6 Comparative Example 6 95.1

[0099] (2) The anti-allergic and soothing composition was placed in a constant temperature environment of -10°C for 24 h respectively, then restored to 25°C and placed for 24 h, and then placed in a constant temperature environment of 50°C for 24 h. The cycle test was continuously carried out for 3 months, and finally the stability was observed. The results are shown in Table 3.

[0100] Table 3 Stability test results

[0101] Test Stability Example 1 No abnormality Example 2 No abnormality Comparative Example 1 Turbidity appears Comparative Example 2 No abnormality Comparative Example 3 Turbidity appears Comparative Example 4 Turbidity appears Comparative Example 5 Turbidity appears Comparative Example 6 Turbidity appears

[0102] From the above performance test results, it can be seen that the extraction rate of avenanthramides by the methods of Examples 1-2 is high, and at the same time, the synergistic soothing effect with the sophora flavescens extract is good, and the storage stability is high.

[0103] In the comparative examples, since the necessary technical solutions were not adopted, their corresponding performance tests were significantly worse than those of the examples. In Comparative Example 1, the soothing effect of the composition obtained by compounding the product prepared with sophora flavescens extract decreased, and the stability was poor. In Comparative Example 2, microwave extraction was not carried out, resulting in a decrease in the extraction rate of avenanthramides. In Comparative Example 3, enzymatic hydrolysis was not carried out, leading to a decrease in the extraction rate, and the synergistic effect with sophora flavescens extract was poor, with poor stability. In Comparative Example 4, different enzymes were used for enzymatic hydrolysis, resulting in a decrease in the extraction rate, and the synergistic effect with sophora flavescens extract was poor, with poor stability. In Comparative Example 5, fermentation was not carried out, resulting in a decrease in the extraction rate, and the synergistic effect with sophora flavescens extract was poor, with poor stability. In Comparative Example 6, different bacteria were used for fermentation, resulting in a decrease in the extraction rate, and the synergistic effect with sophora flavescens extract was poor, with poor stability. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.

[0104] The above are the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. Use of oat-purified alkaloids in the preparation of an anti-allergy and soothing composition, wherein the anti-allergy and soothing composition includes oat-purified alkaloids and sophora flavescens extract, and the purification method of the oat-purified alkaloids includes the following steps: (1) Crush oats to obtain oat powder; (2) Mix the oat powder and water, and place them in a microwave reactor for microwave-assisted extraction to obtain a mixture; (3) Add neutral protease to the mixture, enzymatically hydrolyze at 40°C - 58°C for 40 - 70 min; inactivate the enzyme, adjust the pH value to 7 - 9, add alkaline protease, enzymatically hydrolyze at 40°C - 55°C for 20 - 50 min; adjust the pH value to 5.2 - 7.5, inactivate the enzyme, continue to add ficin, enzymatically hydrolyze at 40°C - 58°C for 10 - 40 min; inactivate the enzyme to obtain an enzymolysis solution; (4) Add a mixed bacterium to the enzymolysis solution for fermentation, sterilize to obtain a fermentation broth; (5) Centrifuge the fermentation broth, subject the solid obtained by centrifugation to subcritical extraction, and then recover the solvent to obtain an extract containing oat alkaloids; The mixed bacterium in step (4) is composed of saccharomyces cerevisiae, lactobacillus gasseri, and lactobacillus helveticus.

2. The use according to claim 1, wherein the dosage of the neutral protease in step (3) is 700 - 800 u / mL of the mixture; the dosage of the alkaline protease is 900 - 1000 u / mL of the mixture; the dosage of the ficin is 500 - 600 u / mL of the mixture.

3. The use according to claim 1, wherein The dosage of the Saccharomyces cerevisiae is 10 8 -10 9 CFU / mL of the enzymolysis solution; the dosage of the Lactobacillus gasseri is 10 8 -10 9 CFU / mL of the enzymolysis solution; the dosage of the Lactobacillus helveticus is 10 6 -10 7 CFU / mL of the enzymolysis solution.

4. The use according to claim 1, wherein the fermentation conditions in step (4) are fermentation at 35 - 38°C for 10 - 14 hours.

5. The use according to claim 1, wherein in step (2), the oat powder and water are mixed at a weight ratio of 1:(10 - 15).

6. The use according to claim 1, wherein in step (1), the oats are crushed to less than 80 mesh.

7. The use according to claim 1, wherein the conditions for microwave-assisted extraction in step (2) are: microwave power 200 - 250 W, extraction time 1 - 2 hours.

8. The use according to claim 1, wherein the conditions for the subcritical extraction in step (5) are: the extractant is propane, the extraction temperature is 50 - 70°C, the pressure is 3 - 4 MPa, and the extractant contains 3 - 4% (by volume) of an entrainer, and the entrainer is ethanol.