Microgel bead coated polypeptide anti-aging composition and preparation method thereof

Abstract

The invention discloses a microgel bead coated polypeptide anti-aging composition and a preparation method thereof. The microgel bead coated polypeptide anti-aging composition comprises a phase A, a phase B and a phase C, the phase A also comprises purified water, a film-forming agent, a thickening agent, glycerol, butanediol, a humectant and sodium hyaluronate; the phase B also comprises beta-glucan, glycosylglycerol, a bletilla striata extract, ergothioneine, a rosa multiflora flower extract, a mussel extract, an antiseptic synergist, hydroxypropyl tetrahydropyrantriol and an emulsifier; the phase C further comprises jojoba oil, sodium alginate, gellan gum, chondrus crispus powder, acetyl hexapeptide-8 and acetyl octapeptide-3; according to the microgel bead coated polypeptide anti-aging composition and the preparation method thereof, acetyl hexapeptide-8 and acetyl octapeptide-3 are coated with microgel beads, so that the effects of acetyl hexapeptide-8 and acetyl octapeptide-3 can be better exerted, a series of unstable problems of active peptides are solved, and the anti-aging effect of the composition is improved. And the two active peptides are complementary to anti-aging and anti-wrinkle products in mechanism, so that the effect that 1 + 1 is greater than 2 is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of beauty and skin care, and in particular to a micro-gel beads encapsulated polypeptide anti-aging combination and a preparation method thereof. Background Art

[0002] Since aging has a complex psychological and physiological impact on people's lives and work, it can cause a series of psychological problems such as anxiety, depression, and inferiority. Therefore, anti-aging care, especially anti-aging care for the skin, has always been one of the focuses of people's demands for skin care products. Among the various manifestations of skin aging, the increase of wrinkles is the most important and obvious change. The formation of wrinkles is the result of changes in both the dermis and epidermis of the skin. During the aging process of the dermis, the number of dermal fibroblasts increases, the proportion of collagen types is unbalanced and the content decreases, the elastic fibers in the papillary layer and reticular layer of the dermis decrease, and the disorder of the arrangement of collagen fibers and elastic fibers can all lead to the formation of wrinkles. In addition, with the increase of age, the number of keratinocytes in the epidermis decreases, and the content of natural moisturizing factors in the stratum corneum decreases, resulting in a decrease in the skin's hydration ability, causing the skin tissue cells to shrink and age, and tissue morphological changes occur, which can eventually manifest as the formation of wrinkles on the skin surface.

[0003] Based on the above reasons, a good anti-aging skin care product should meet three functions at the same time: 1) It can remove excessive reactive oxygen free radicals in the dermis, promote cell metabolism, and stimulate the production of collagen in the skin. 2) It can inhibit the release of the neurotransmitter acetylcholine in the basal layer of the epidermis, thereby reducing muscle contraction. 3) It can repair the skin barrier function in the stratum corneum of the epidermis, promote the integrity of the skin structure, and maintain the skin's natural water retention capacity. Since skin aging is caused by both the dermis and the epidermis, although anti-aging products developed based on a single mechanism can play a certain anti-aging effect, the overall effect is poor. Therefore, in order to develop anti-aging products with more outstanding effects, it is necessary to delay skin aging at multiple levels and angles, targeting both the dermis and the epidermis at the same time.

[0004] Affected by the epidermal barrier and the physical and chemical properties of raw materials, most active ingredients in conventional products stay in the stratum corneum or epidermis, and it is difficult for them to penetrate into the dermis to repair aging cells. Therefore, under the premise of ensuring the activity of raw materials, improving the permeability and absorption coefficient of the formula and accelerating the transdermal rate are the keys to improving the anti-aging effect of skin care products.

[0005] The prior art has the following defects or problems:

[0006] 1. Microemulsion, which is currently commonly used in topical drug preparations, is an excellent transdermal carrier. It is a transparent or translucent, low-viscosity, thermodynamically stable oil-water mixed system with a particle size usually between 10-100nm, which is spontaneously formed by water phase, oil phase, surfactant and co-surfactant in appropriate proportions. Because microemulsion droplets are both lipophilic and hydrophilic, they have good compatibility with the intercellular lipid bilayer of the stratum corneum. By increasing the fluidity of the stratum corneum lipid bilayer, the active ingredients in the essence can easily penetrate the stratum corneum to reach the epidermis. Although microemulsion has good transdermal properties, due to the large amount of surfactants and co-surfactants used in the microemulsion formula, it has a certain damage to the stratum corneum of the skin. Therefore, skin care products prepared using the microemulsion process alone not only cannot achieve the anti-aging effect of the epidermis, but long-term use will make the skin sensitive due to excessive stimulation;

[0007] 2. At present, the research and development of anti-wrinkle and anti-aging products is a hot topic in cosmetic skin care. The purpose of anti-wrinkle and anti-aging is usually achieved by adding active ingredients. The commonly used active ingredients are peptides, but most of the peptides on the market are water-soluble liquids with relatively low content and poor stability. The most common problems are:

[0008] a. Easy to oxidize and deteriorate;

[0009] b. Easily hydrolyzed, resulting in peptide chain breakage and loss of activity;

[0010] c. Short peptide chains are very susceptible to polymerization, which results in the recombination of peptide chains into another substance;

[0011] d. The solvent preservatives in the polypeptide solution may cause skin allergies, redness and other symptoms, posing safety issues.

[0012] It should be noted that the above contents belong to the technical knowledge of the inventor and do not necessarily constitute prior art. Summary of the invention

[0013] In view of the deficiencies in the prior art, the present invention provides a microgel beads-encapsulated polypeptide anti-aging combination and a preparation method thereof, which solves the existing problems.

[0014] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a microgel beads encapsulated polypeptide anti-aging combination, comprising phase A, phase B and phase C;

[0015] The phase A also includes the following raw materials in parts by weight:

[0016] Purified water 50-90%, film former 0.1-0.2%, xanthan gum 0.01-0.05%, thickener 0.01-0.05%, glycerin 0.5-1.5%, butylene glycol 1-3%, humectant 0.1-0.6% and sodium hyaluronate 0.01-0.05%;

[0017] The phase B also includes the following raw materials in parts by weight:

[0018] β-glucan 1-3%, glyceryl glucoside 3-5%, bletilla striata extract 1-3%, ergothioneine 0.01-0.05%, rosa centifolia flower extract 0.1-0.05%, mussel extract 0.01-0.05%, preservative synergist 0.8-1%, hydroxypropyl tetrahydropyrantriol 0.1-1% and emulsifier 0.15-0.2%;

[0019] The phase C also includes the following raw materials in parts by weight:

[0020] Jojoba oil 5-10%, sodium alginate 0.1-1%, gellan gum 0.1-1%, crispy chondrus crispus powder 0.1-1%, acetyl hexapeptide-8 0.005-0.1% and acetyl octapeptide-3 0.005-0.1%.

[0021] As a preferred technical solution of the present invention, the film-forming agent is specifically selected from acrylic acid (ester) heat / C10-30 alkane acrylate cross-linked polymer, the thickener is specifically selected from acrylate cross-linked polymer-6, and the humectant is specifically selected from 1,2-ethylene glycol;

[0022] The antiseptic synergist is specifically selected from caprylhydroxamic acid, and the emulsifier is specifically selected from triethanolamine.

[0023] Another technical problem to be solved by the present invention is to provide a method for preparing a microgel bead-encapsulated polypeptide anti-aging combination, comprising the following steps:

[0024] Step 1: Weigh the raw materials of phase A, phase B and phase C, place the raw materials of phase A into a stirring pot, and disperse them evenly through an external stirring device;

[0025] Step 2: Heat in a water bath to a specific temperature, then stir and dissolve until clear;

[0026] Step 3: After stirring and cooling to a specific temperature, add the raw materials of phase B and further stir and mix. The material body is vacuumed until it is clear and transparent without bubbles. After the physical and chemical tests are qualified, the material is discharged into a sterile container;

[0027] Step 4: Slowly add the Phase C raw materials into the sterile container, use a stirring rod to slowly stir until the material and micro-gel beads are fully mixed without bubbles, and let it stand for 24 hours;

[0028] Step 5: Conduct physical, chemical and microbiological tests on the material, and fill it after passing the test.

[0029] Furthermore, the water bath heating temperature needs to be controlled at 80-85 degrees Celsius, and the temperature needs to be kept stable. During the water bath, the water level needs to be maintained between 2 / 3 and 3 / 4 of the container. A cover needs to be added during the water bath to prevent the water level from dropping due to evaporation.

[0030] Furthermore, the stirring temperature needs to be lowered to 40-45 degrees Celsius;

[0031] The vacuum treatment is achieved by using a vacuum pump. During the vacuum treatment, it is necessary to observe whether bubbles appear on the surface of the liquid. If bubbles appear, the container is slightly shaken to help the bubbles rise and release. The pressure needs to be controlled between 1-50 Torr.

[0032] Furthermore, the stirring rod is made of polytetrafluoroethylene.

[0033] Compared with the prior art, the present invention provides a microgel beads encapsulated polypeptide anti-aging combination and a preparation method thereof, which has the following beneficial effects:

[0034] The invention discloses a micro-bead-encapsulated polypeptide anti-aging combination and a preparation method thereof. In the invention, acetyl hexapeptide-8 and acetyl octapeptide-3 are encapsulated in micro-beads. In order to better exert the effects of acetyl hexapeptide-8 and acetyl octapeptide-3, a series of instability problems of active peptides are solved by using micro-bead encapsulation technology, and the two active peptides complement each other in anti-aging and anti-wrinkle products in terms of mechanism, thereby achieving the effect of 1+1>2.

[0035] Richer functional ingredients and better effects: The present invention is compounded by the above anti-wrinkle and firming ingredients in proportion. Compared with the existing anti-aging ingredients, its advantages are that these ingredients cover the main pathways of skin aging, remove free radicals, promote cell metabolism, stimulate skin collagen regeneration, repair skin barrier, replenish water and lock moisture, and can better achieve anti-wrinkle and firming. The raw materials used are safe and non-irritating and will not cause damage to human skin.

[0036] The formula system is matched, and the entire water-oil essence system is a transparent essence without surfactants and co-surfactants. Micro-gel beads are suspended in the essence, which makes it more refreshing and non-greasy to use. In addition, the ultra-high value and transparent texture of micro-gel beads are enough to attract those who are obsessed with appearance. Different from the traditional water-oil mixed opaque appearance, it allows consumers to get a visual product experience, which is safer and more reliable to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is a schematic diagram of the anti-aging combination state of the microgel beads encapsulating the polypeptide of the present invention;

[0038] Figure 2 This is a schematic diagram of another state of the microgel beads encapsulating the polypeptide anti-aging combination of the present invention;

[0039] Figure 3 This is a schematic diagram of the Antera3D pore density test results of the present invention;

[0040] Figure 4 This is a bar graph of the pore density test of the present invention;

[0041] Figure 5 A schematic diagram of a pore density image obtained by Antera3D in the present invention;

[0042] Figure 6 It is a schematic diagram of the elasticity test results of the Ballistometer of the present invention;

[0043] Figure 7 This is a bar graph of the skin relaxation test of the present invention;

[0044] Figure 8 This is a schematic diagram of the Antera3D pore depth test results of the present invention;

[0045] Fig. 9 This is a bar graph of the pore depth test of the present invention;

[0046] Fig.10 A schematic diagram of a pore depth image obtained by Antera3D in the present invention;

[0047] Fig.11 Schematic diagram of the test results of pore volume and depth of Antera3D of the present invention;

[0048] Fig.12 This is a bar graph of the total pore volume test of the present invention;

[0049] Fig.13 This is a histogram of the deepest part of the pores tested in the present invention;

[0050] Fig.14 Schematic diagram of pore volume and depth images obtained by Antera3D of the present invention;

[0051] Fig.15 This is a schematic diagram of the efficacy feedback after the present invention is used once;

[0052] Fig.16 This is a schematic diagram of the efficacy feedback after the present invention is used ten times;

[0053] Fig.17 This is a schematic diagram of the feedback results of skin abnormalities of the experimental subjects of the present invention. DETAILED DESCRIPTION

[0054] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0055] See also Figure 1-17 , in embodiment 1:

[0056] A micro-gel beads encapsulated polypeptide anti-aging combination, including phase A, phase B and phase C;

[0057] Phase A also includes the following raw materials in parts by weight: 50-90% purified water, 0.1-0.2% acrylates / C10-30 alkane acrylate cross-linked polymer, 0.01-0.05% acrylate cross-linked polymer-6, 0.5-1.5% glycerol, 1-3% butylene glycol, 0.1-0.6% 1,2-ethylene glycol and 0.01% sodium hyaluronate;

[0058] Phase B also includes the following raw materials in parts by weight:

[0059] β-Glucan 1%, Glyceryl Glucoside 3%, Rosa Centifolia Flower Extract 0.1-0.05%, Mussel Extract 0.01%, Caprylhydroxamic Acid 0.8-1% and Triethanolamine 0.15-0.2%;

[0060] Phase C also includes the following raw materials in parts by weight:

[0061] Jojoba oil 5-10%, sodium alginate 0.1-1%, gellan gum 0.1-1%, crispy chondrus crispus powder 0.1-1%, acetyl hexapeptide-8 0.005% and acetyl octapeptide-3 0.0055%.

[0062] Based on the above-mentioned micro-gel beads encapsulated polypeptide anti-aging combination, a preparation method of the micro-gel beads encapsulated polypeptide anti-aging combination is proposed, comprising the following steps:

[0063] Step 1: Weigh the raw materials of phase A, phase B and phase C, place the raw materials of phase A into a stirring pot, and disperse them evenly through an external stirring device;

[0064] The water bath heating temperature must be controlled at 80 degrees Celsius and the temperature needs to be kept stable. The water level must be kept between 2 / 3 of the container during the water bath. A cover must be added during the water bath to prevent the water level from dropping due to evaporation.

[0065] Step 2: Heat in a water bath to a specific temperature, then stir and dissolve until clear;

[0066] Step 3: After stirring and cooling to a specific temperature, add the raw materials of phase B and further stir and mix. The material body is vacuumed until it is clear and transparent without bubbles. After the physical and chemical tests are qualified, the material is discharged into a sterile container;

[0067] The stirring temperature needs to be lowered to 40 degrees Celsius; the vacuum treatment is achieved using a vacuum pump. During the vacuum process, it is necessary to observe whether bubbles appear on the surface of the liquid. If bubbles appear, shake the container slightly to help the bubbles rise and release. The pressure needs to be controlled at 35 Torr.

[0068] Step 4: Slowly add the Phase C raw materials into the sterile container, use a stirring rod to slowly stir until the material and micro-gel beads are fully mixed without bubbles, and let it stand for 24 hours;

[0069] The stirring rod is made of polytetrafluoroethylene.

[0070] Step 5: Conduct physical, chemical and microbiological tests on the material, and fill it after passing the test

[0071] The size of the micro-gel beads prepared in this embodiment is 0.01-0.03 mm in diameter. The core component micro-gel beads encapsulate acetyl hexapeptide-8 and acetyl octapeptide-3, and are compounded with sodium hyaluronate, glyceryl glucoside, β-glucan, and Rosa centifolia flower extract to achieve an anti-aging, moisturizing and water-locking effect.

[0072] See also Figure 1-17 , in the second embodiment:

[0073] A micro-gel beads encapsulated polypeptide anti-aging combination, including phase A, phase B and phase C;

[0074] Phase A also includes the following raw materials in parts by weight: 50-90% purified water, 0.1-0.2% acrylates / C10-30 alkane acrylate cross-linked polymer, 0.01-0.05% acrylate cross-linked polymer-6, 0.5-1.5% glycerol, 1-3% butylene glycol, 0.1-0.6% 1,2-ethylene glycol and 0.03% sodium hyaluronate;

[0075] Phase B also includes the following raw materials in parts by weight:

[0076] Bletilla striata extract 2%, Rosa centifolia flower extract 0.1-0.05%, caprylhydroxamic acid 0.8-1%, hydroxypropyl tetrahydropyrantriol 0.1-1% and triethanolamine 0.15-0.2%;

[0077] Phase C also includes the following raw materials in parts by weight:

[0078] Jojoba oil 5-10%, sodium alginate 0.1-1%, gellan gum 0.1-1%, crispy chondrus crispus powder 0.1-1%, acetyl hexapeptide-8 0.008% and acetyl octapeptide-3 0.008%.

[0079] Based on the above-mentioned micro-gel beads encapsulated polypeptide anti-aging combination, a preparation method of the micro-gel beads encapsulated polypeptide anti-aging combination is proposed, comprising the following steps:

[0080] Step 1: Weigh the raw materials of phase A, phase B and phase C, place the raw materials of phase A into a stirring pot, and disperse them evenly through an external stirring device;

[0081] The water bath heating temperature must be controlled at 85 degrees Celsius and the temperature needs to be kept stable. The water level must be kept between 2 / 3 of the container during the water bath. A cover must be added during the water bath to prevent the water level from dropping due to evaporation.

[0082] Step 2: Heat in a water bath to a specific temperature, then stir and dissolve until clear;

[0083] Step 3: After stirring and cooling to a specific temperature, add the raw materials of phase B and further stir and mix. The material body is vacuumed until it is clear and transparent without bubbles. After the physical and chemical tests are qualified, the material is discharged into a sterile container;

[0084] The stirring temperature needs to be lowered to 42 degrees Celsius; the vacuum treatment is achieved using a vacuum pump. During the vacuum process, it is necessary to observe whether bubbles appear on the surface of the liquid. If bubbles appear, shake the container slightly to help the bubbles rise and release. The pressure needs to be controlled at 40Torr.

[0085] Step 4: Slowly add the Phase C raw materials into the sterile container, use a stirring rod to slowly stir until the material and micro-gel beads are fully mixed without bubbles, and let it stand for 24 hours;

[0086] The stirring rod is made of polytetrafluoroethylene.

[0087] Step 5: Conduct physical, chemical and microbiological tests on the material, and fill it after passing the test.

[0088] The diameter of the micro-gel beads prepared in this embodiment is 0.05-0.1mm. The core functional ingredient micro-gel beads are wrapped with acetyl hexapeptide-8 and acetyl octapeptide-3, and are compounded in proportion with other functional raw materials such as Bletilla striata extract and hydroxypropyl tetrahydropyrantriol, which can better achieve anti-aging and firming effects, are safe and non-irritating, and are more suitable for human skin.

[0089] See also Figure 1-17 , in Example 3:

[0090] A micro-gel beads encapsulated polypeptide anti-aging combination, including phase A, phase B and phase C;

[0091] Phase A also includes the following raw materials in parts by weight: 50-90% purified water, 0.1-0.2% acrylate (ester) heat / C10-30 alkane acrylate cross-linked polymer, 0.01-0.05% xanthan gum, 0.5-1.5% glycerol, 1-3% butylene glycol, 0.1-0.6% 1,2-ethylene glycol and 0.05% sodium hyaluronate;

[0092] Phase B also includes the following raw materials in parts by weight:

[0093] Ergothioneine 0.01-0.05%, Rosa centifolia flower extract 0.1-0.05%, caprylhydroxamic acid 0.8-1% and triethanolamine 0.15-0.2%;

[0094] Phase C also includes the following raw materials in parts by weight:

[0095] Jojoba oil 5-10%, sodium alginate 0.1-1%, gellan gum 0.1-1%, crispy chondrus crispus powder 0.1-1%, acetyl hexapeptide-8 0.01% and acetyl octapeptide-3 0.01%.

[0096] Based on the above-mentioned micro-gel beads encapsulated polypeptide anti-aging combination, a preparation method of the micro-gel beads encapsulated polypeptide anti-aging combination is proposed, comprising the following steps:

[0097] Step 1: Weigh the raw materials of phase A, phase B and phase C, place the raw materials of phase A into a stirring pot, and disperse them evenly through an external stirring device;

[0098] The water bath heating temperature must be controlled at 85 degrees Celsius and the temperature needs to be kept stable. The water level must be kept between 2 / 3 of the container during the water bath. A cover must be added during the water bath to prevent the water level from dropping due to evaporation.

[0099] Step 2: Heat in a water bath to a specific temperature, then stir and dissolve until clear;

[0100] Step 3: After stirring and cooling to a specific temperature, add the raw materials of phase B and further stir and mix. The material body is vacuumed until it is clear and transparent without bubbles. After the physical and chemical tests are qualified, the material is discharged into a sterile container;

[0101] The stirring temperature needs to be lowered to 42 degrees Celsius; the vacuum treatment is achieved using a vacuum pump. During the vacuum process, it is necessary to observe whether bubbles appear on the surface of the liquid. If bubbles appear, shake the container slightly to help the bubbles rise and release. The pressure needs to be controlled at 45 Torr.

[0102] Step 4: Slowly add the Phase C raw materials into the sterile container, use a stirring rod to slowly stir until the material and micro-gel beads are fully mixed without bubbles, and let it stand for 24 hours;

[0103] The stirring rod is made of polytetrafluoroethylene.

[0104] Step 5: Conduct physical, chemical and microbiological tests on the material, and fill it after passing the test.

[0105] The diameter of the micro-gel beads prepared in this embodiment is 0.05-0.1 mm. The core functional ingredient micro-gel beads are wrapped with acetyl hexapeptide-8 and acetyl octapeptide-3, and are combined with ergothioneine, so that the product can achieve the effects of firming and anti-aging, as well as anti-oxidation and skin brightening, and is safe and non-irritating, and is more suitable for human skin.

[0106] Each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiment.

[0107] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A micro-gel beads encapsulated polypeptide anti-aging combination, characterized in that: Including phase A, phase B and phase C; The phase A also includes the following raw materials in parts by weight: Purified water 50-90%, film former 0.1-0.2%, xanthan gum 0.01-0.05%, thickener 0.01-0.05%, glycerin 0.5-1.5%, butylene glycol 1-3%, humectant 0.1-0.6% and sodium hyaluronate 0.01-0.05%; The phase B also includes the following raw materials in parts by weight: β-glucan 1-3%, glyceryl glucoside 3-5%, bletilla striata extract 1-3%, ergothioneine 0.01-0.05%, rosa centifolia flower extract 0.1-0.05%, mussel extract 0.01-0.05%, preservative synergist 0.8-1%, hydroxypropyl tetrahydropyrantriol 0.1-1% and emulsifier 0.15-0.2%; The phase C also includes the following raw materials in parts by weight: Jojoba oil 5-10%, sodium alginate 0.1-1%, gellan gum 0.1-1%, crispy chondrus crispus powder 0.1-1%, acetyl hexapeptide-8 0.005-0.1% and acetyl octapeptide-3 0.005-0.1%.

2. The anti-aging combination of microgel beads encapsulated with polypeptides according to claim 1, characterized in that: The film-forming agent is specifically selected from acrylic acid (ester) heat / C10-30 alkane acrylate cross-linked polymer, the thickener is specifically selected from acrylate cross-linked polymer-6, and the moisturizing agent is specifically selected from 1,2-ethylene glycol; The antiseptic synergist is specifically selected from caprylhydroxamic acid, and the emulsifier is specifically selected from triethanolamine.

3. A method for preparing a micro-gel beads-encapsulated polypeptide anti-aging combination, characterized in that: The steps include: Step 1: Weigh the raw materials of phase A, phase B and phase C, place the raw materials of phase A into a stirring pot, and disperse them evenly through an external stirring device; Step 2: Heat in a water bath to a specific temperature, then stir and dissolve until clear; Step 3: After stirring and cooling to a specific temperature, add the raw materials of phase B and further stir and mix. The material body is vacuumed until it is clear and transparent without bubbles. After the physical and chemical tests are qualified, the material is discharged into a sterile container; Step 4: Slowly add the Phase C raw materials into the sterile container, use a stirring rod to slowly stir until the material and micro-gel beads are fully mixed without bubbles, and let it stand for 24 hours; Step 5: Conduct physical, chemical and microbiological tests on the material, and fill it after passing the test.

4. The method for preparing a microgel beads-encapsulated polypeptide anti-aging combination according to claim 3, characterized in that: The water bath heating temperature in step 2 needs to be controlled at 80-85 degrees Celsius, and the temperature needs to be kept stable. The water level needs to be maintained between 2 / 3 and 3 / 4 of the container during the water bath. A cover needs to be added during the water bath to prevent the water level from dropping due to evaporation.

5. The method for preparing a microgel beads-encapsulated polypeptide anti-aging combination according to claim 3, characterized in that: The stirring temperature in step 3 needs to be reduced to 40-45 degrees Celsius; The vacuum treatment is achieved by using a vacuum pump. During the vacuum treatment, it is necessary to observe whether bubbles appear on the surface of the liquid. If bubbles appear, the container is slightly shaken to help the bubbles rise and release. The pressure needs to be controlled between 1-50 Torr.

6. The method for preparing a microgel beads-encapsulated polypeptide anti-aging combination according to claim 3, characterized in that: The stirring rod described in step 4 is made of polytetrafluoroethylene.

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