Method and device for efficiently collecting entomopathogenic nematodes

Abstract

The invention relates to the field of biopesticides, in particular to a method and device for efficiently collecting entomopathogenic nematodes, and the method comprises the following steps: putting a nematode carrier of which the density is smaller than that of water into water, and standing for a certain time; the nematode carrier is separated from the nematodes, the nematode carrier floats on the water surface, and the nematodes sink to the lower part; cleaning the nematode carrier floating on the water surface; separating upper-layer water from the sunk nematodes; and collecting the nematodes. The efficient collection method and device for the entomopathogenic nematodes are invented through matched devices, by utilizing the biological characteristics of the entomopathogenic nematodes and by combining equipment manufacturing and design, large-area application of a rice green prevention and control technology system is promoted, and the efficient collection method and device are one of effective ways for achieving zero increase of the pesticide use amount.

Description

Technical Field

[0001] The invention relates to the field of biological pesticides, and in particular to a method and a device for efficiently collecting insect pathogenic nematodes. Background Art

[0002] The farming industry is still an indispensable part of human activities at this stage. Pesticides play an indispensable role in the farming process. The losses of agricultural diseases, insects and weeds avoided and recovered by pesticides account for 1 / 3 of the grain output worldwide. However, the long-term use of pesticides has not only caused serious environmental pollution and destroyed biodiversity, but also caused tens of thousands of poisoning incidents and economic losses. On the other hand, physical and biological means can also play a good role in preventing and controlling diseases, insects and weeds in the agricultural production process. By developing new methods and new devices for artificial reproduction and release of natural enemies to replace chemical pesticides, the use of pesticides in the rice planting process can be greatly reduced.

[0003] Insect pathogenic nematodes can be parasitoids or predators, and can also be called pathogenic microorganisms. As parasitoids or predators, they have active chemical receptors; as pathogens, they have high toxicity, can quickly kill hosts, and have high reproduction potential. They have a wide host range, are safe for vertebrates, plants and other non-target organisms, can be mixed with many chemical pesticides, and can withstand genetic selectivity. The traditional method uses sponges as carriers to breed nematodes, and the newly bred nematodes (sponges) are directly placed in cold storage for storage and taken out when crop pests are needed. At this time, the amount of nematodes per gram of sponge is 150,000-180,000, and the sponge is not fully utilized because the sponge can absorb more nematodes, and the storage and transportation process occupies a large space. Secondly, the newly bred nematodes (sponges) are prone to odor during storage because there is still a certain amount of feed and impurities in them. Thirdly, when releasing nematodes in the field, the nematodes need to be precipitated on the spot at the edge of the field, and the operation process is labor-intensive and time-consuming. Summary of the invention

[0004] The purpose of the present invention is to provide a method and device for efficiently collecting insect pathogenic nematodes to solve the above-mentioned problems. The present invention utilizes the biological characteristics of insect pathogenic nematodes and combines equipment manufacturing and design to invent a method and device for efficiently collecting insect pathogenic nematodes, which is conducive to promoting the large-scale application of rice green control technology system and is one of the effective ways to achieve "zero growth" in pesticide use.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] A method for efficiently collecting insect pathogenic nematodes comprises the following steps:

[0007] Place a nematode carrier with a density lower than that of water into water and let it stand for a certain period of time;

[0008] The nematode carrier is separated from the nematodes, the nematode carrier floats on the water surface, and the nematodes sink to the bottom;

[0009] cleaning the nematode carrier floating on the water surface;

[0010] Separate the upper water layer from the sinking nematodes;

[0011] Collect nematodes.

[0012] Preferably, the nematode carrier having a density less than that of water is placed in water and allowed to stand for 1-2 hours.

[0013] Preferably, the nematode carrier is a sponge.

[0014] An efficient collection device for insect pathogenic nematodes, used to implement the above method, the collection device comprising:

[0015] A water tank for holding clean water;

[0016] A sedimentation tank is arranged below the water storage tank, has an open top and is connected to the bottom of the water storage tank;

[0017] An isolation component, used for separating nematodes from the nematode carrier;

[0018] The drainage component is arranged on the bottom of the sedimentation tank and is used to discharge the water in the nematodes after they sink.

[0019] Preferably, the side wall of the water storage tank is connected to a water inlet valve, which is used to separate the nematode carrier from the nematodes. The side wall of the water storage tank is connected to a drain valve, which is used to drain the upper water of the nematodes.

[0020] Preferably, the isolation component is a dustproof net, and the particle size of the dustproof net allows the nematodes to enter the sedimentation tank.

[0021] Preferably, a driving component for driving the nematodes to swim downward is provided above the water storage tank.

[0022] Preferably, the driving component is an electric lamp.

[0023] Preferably, the height of the drainage component is adjustable, and a drainage outlet is provided at the bottom end of the drainage component.

[0024] Preferably, the drainage component is a telescopic pipe.

[0025] The present invention has the following technical effects:

[0026] Through the nematode collection method and device of the present invention, the amount of nematodes in each gram of nematode carrier is increased, thereby reducing the storage volume and quality, and being more convenient for transportation and field release, greatly reducing the application cost of insect pathogenic nematodes, and being more easily accepted by the market. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0028] Figure 1 It is a schematic diagram of the main structure of the device of the present invention;

[0029] Figure 2 It is a schematic diagram of the top structure of the device of the present invention.

[0030] Among them, 1. water storage tank; 2. sedimentation tank; 3. isolation component; 4. water inlet valve; 5. drain valve; 6. drain component; 7. drain outlet; 8. electric light. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0033] Reference Figure 1 to Figure 2 As shown, this embodiment provides a method for efficiently collecting insect pathogenic nematodes, comprising the following steps:

[0034] Place a nematode carrier with a density lower than that of water into water and let it stand for a certain period of time;

[0035] The nematode carrier is separated from the nematode, the nematode carrier floats on the water surface, and the nematode sinks to the bottom;

[0036] Clean up nematode carriers that float to the surface of the water;

[0037] Separate the upper water layer from the sinking nematodes;

[0038] Collect nematodes.

[0039] Using the principle that nematodes will sink in water due to their own weight, the newly bred nematode carrier containing nematodes is placed in a container filled with clean water. After standing for a period of time, the nematodes in the nematode carrier sink to the bottom of the water, and the nematode carrier floats on the water surface, thereby separating the nematodes from the nematode carrier; the upper nematode carrier is cleaned off, and the upper clean water is poured out, and the clean nematode carrier is used to fully absorb the nematodes that have sunk, so as to maximize the nematode density in the nematode carrier.

[0040] To further optimize the solution, a nematode carrier with a density lower than that of water is placed into the water and allowed to stand for 1-2 hours.

[0041] In a further optimized solution, the nematode carrier is a sponge.

[0042] An efficient collection device for insect pathogenic nematodes, used to implement the above method, the collection device comprising:

[0043] The water holding tank 1 is used for holding clean water; and the nematodes in the sponge with nematodes will settle downwards.

[0044] The sedimentation tank 2 is arranged below the water storage tank 1, has an open top and is connected to the bottom of the water storage tank 1;

[0045] An isolation component 3, used for separating nematodes and nematode carriers;

[0046] The drainage component 6 is arranged at the bottom of the sedimentation tank 2 and is used to discharge the water in the nematodes after they sink.

[0047] By utilizing the weight of the nematodes themselves, the nematodes come out of the sponge and are precipitated into the sedimentation tank 2. The sedimentation tank 2 may have a spherical structure made of a smooth material (such as stainless steel) to facilitate the nematodes in the sedimentation tank 2 to be discharged from the drainage component 6 more quickly.

[0048] According to a further optimization scheme, the side wall of the water storage tank 1 is connected to a water inlet valve 4, which is used for the water used to separate the nematode carrier from the nematodes. The side wall of the water storage tank 1 is connected to a drain valve 5, which is used to discharge the upper layer of water of the nematodes.

[0049] The water inlet valve 4 is used to soak the sponge and the clean water for precipitating the nematodes enters the water storage tank 1 and the sedimentation tank 2 from the water inlet valve 4; the drainage valve 5: after all the nematodes in the sponge are precipitated, open the drainage valve 5 to quickly drain the clean water above the nematodes.

[0050] According to a further optimized solution, the isolation component 3 is a dustproof net, and the particle size of the dustproof net allows the nematodes to enter the sedimentation tank 2.

[0051] Isolation component 3: prevents the sponge from sinking into the sedimentation tank 2, thereby affecting the purity of the nematodes. The anti-sinking net has a diameter of 2-3mm and can be shaken up and down to speed up the sinking speed of the nematodes in the sponge.

[0052] To further optimize the solution, a driving component for driving the nematodes to swim downward is provided above the water storage tank 1.

[0053] According to a further optimized solution, the driving component is an electric lamp 8.

[0054] Light 8: Taking advantage of the light-avoidance of nematodes, a weak light source is turned on by light 8 to accelerate the dissolution and sinking of nematodes in the sponge.

[0055] According to a further optimized solution, the height of the drainage component 6 is adjustable, and a drainage outlet 7 is provided at the bottom end of the drainage component 6.

[0056] Drainage port 7: After the excess water is discharged through the drainage component 6, the settled nematodes are discharged and collected through the drainage port 7;

[0057] According to a further optimized solution, the drainage component 6 is a telescopic tube.

[0058] The drainage component 6 is configured as a telescopic tube: after the upper layer of clean water is discharged through the drainage valve 5, the water level is precisely controlled by the drainage component 6 to discharge as much excess water as possible without discharging nematodes.

[0059] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0060] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for efficiently collecting insect pathogenic nematodes, characterized in that: The steps include: Place a nematode carrier with a density lower than that of water into water and let it stand for a certain period of time; The nematode carrier is separated from the nematodes, the nematode carrier floats on the water surface, and the nematodes sink to the bottom; cleaning the nematode carrier floating on the water surface; Separate the upper water layer from the sinking nematodes; Collect nematodes.

2. The method for efficiently collecting insect pathogenic nematodes according to claim 1, characterized in that: Place a nematode carrier with a density lower than that of water into the water and let it stand for 1-2 hours.

3. The method for efficiently collecting insect pathogenic nematodes according to claim 1, characterized in that: The nematode carrier is a sponge.

4. An efficient collection device for insect pathogenic nematodes, used for implementing the method according to any one of claims 1 to 3, characterized in that: The collecting device comprises: A water storage tank (1) for storing clean water; A sedimentation tank (2) is arranged below the water storage tank (1), has an open top, and is connected to the bottom of the water storage tank (1); An isolation component (3) for separating the nematodes from the nematode carrier; A drainage component (6) is arranged on the bottom surface of the sedimentation tank (2) and is used to drain the water in the nematodes after they sink.

5. The efficient collection device for insect pathogenic nematodes according to claim 4, characterized in that: The side wall of the water storage tank (1) is connected to a water inlet valve (4), and the water inlet valve (4) is used for the water used to separate the nematode carrier from the nematodes. The side wall of the water storage tank (1) is connected to a water discharge valve (5), and the water discharge valve (5) is used to discharge the upper layer of water of the nematodes.

6. The efficient collection device for insect pathogenic nematodes according to claim 4, characterized in that: The isolation component (3) is a dustproof net, and the particle size of the dustproof net allows the nematodes to enter the sedimentation tank (2).

7. The efficient collection device for insect pathogenic nematodes according to claim 4, characterized in that: A driving component for driving the nematodes to swim downwards is provided above the water storage pool (1).

8. The efficient collection device for insect pathogenic nematodes according to claim 7, characterized in that: The driving component is an electric lamp (8).

9. The efficient collection device for insect pathogenic nematodes according to claim 4, characterized in that: The height of the drainage component (6) is adjustable, and a drainage outlet (7) is provided at the bottom end of the drainage component (6).

10. The efficient collection device for insect pathogenic nematodes according to claim 9, characterized in that: The drainage component (6) is a telescopic pipe.

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