Production method of BVDV and IBRV genetic engineering subunit fusion protein

CN119613564APending Publication Date: 2025-03-14SHANGHAI SHEN LIAN BIOMEDICAL CORP
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Patent Information

Application Number
CN202411592985.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-08
Publication Date
2025-03-14

AI Technical Summary

Technical Problem

Current methods for producing BVDV and IBRV subunit vaccines are complex and costly due to the need for separate expression and purification of individual proteins, and existing CHO cell cultivation methods result in low cell density, low expression levels, and high production costs when attempting to produce BVDV and IBRV gene engineering subunit fusion proteins.

Method used

A CHO cell cultivation method involving specific seeding density, temperature adjustments, and supplementation schedules using defined media components to maintain high cell viability and density, optimizing the expression of BVDV and IBRV gene engineering subunit fusion proteins, thereby enhancing production efficiency and reducing costs.

Benefits of technology

The method achieves high cell density and viability, resulting in increased protein expression levels up to 2.9 g/L, simplifying industrial-scale production and reducing production costs while maintaining high cell activity.

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Abstract

The invention discloses a production method of BVDV and IBRV genetic engineering subunit fusion protein, which comprises the following steps: inoculating CHO seed cells of fusion expression BVDV-IBRV protein to a basic culture medium, carrying out feed supplement culture, inoculating on the 0th day, supplementing 3% of feed every day from the 1st day to the 6th day, then supplementing 3% of feed every other day, and finishing the feed supplement on the 12th day. Due to the fact that CHO cell genes are prone to denaturation, in order to express different foreign proteins, different culture conditions, different culture media, different material supplementing modes and the like can influence the density, activity and metabolism of CHO cells, the final target yield and the like.
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Citation Information

Patent Citations

  • Preparation of CHO cell expressed infectious bovine rhinotracheitis virus protein gD and subunit vaccine thereof and application

    CN107973840A

  • Culture method and application of CHO cell line for efficient secretion of classical swine fever E2 protein

    CN107974432B