Immunochromatography reagent card detection method, storage medium and system

Abstract

The invention provides an immunochromatography reagent card detection method, a storage medium and a system. The method comprises the following steps: a reaction line comprises a quality control line C and a detection line T; scanning the test strip to obtain a one-dimensional fluorescence signal curve, wherein the one-dimensional fluorescence signal curve is at least used for representing a fluorescence signal of the quality control line C and a fluorescence signal of the detection line T; a fluorescence signal of the detection line T is at least used for determining a first detection result of the to-be-detected sample; judging whether the reagent card is abnormal or not at least based on the wave crest number and / or the peak value and / or the area and / or the full width at half maximum of the characteristic peak of the one-dimensional fluorescence signal curve; when it is judged that the reagent card is not abnormal, a first detection result is output; and when it is judged that the reagent card is abnormal, at least outputting first abnormal prompt information and / or executing a detection result confirmation operation. According to the embodiment of the invention, the detection precision of the immunochromatography reagent card is improved.

Description

Technical Field

[0001] The present invention relates to the field of intelligent detection technology, and in particular to an immunochromatography reagent card detection method, a storage medium and a system. Background Art

[0002] Immunochromatography is a technology that uses the principle of chromatography to quickly analyze substances with antigen-antibody specific immune reactions. Immunochromatography is divided into two categories: interpretation under visible light and interpretation under excitation light. The former is represented by colloidal gold immunochromatography, which is widely used in qualitative detection by naked eye interpretation, and sometimes also used for semi-quantitative / quantitative detection by instrument interpretation. The latter is represented by fluorescent immunochromatography, which is mainly used for quantitative detection. The detection results of immunochromatography are presented through reaction lines (detection line T and quality control line C). Before the detection, the reaction area is usually in a blank background state. After the detection, a reaction line different from the background appears in the reaction area. The color / fluorescence signal difference between the reaction line and the background represents the accumulation degree of the reaction substance, which has a corresponding relationship with the content of the substance to be tested. By establishing a "standard content-color / fluorescence signal" curve (called a "standard curve") with a standard sample of known content, the quantitative result of the substance to be tested in the sample can be calculated. Therefore, accurate analysis of the color / fluorescence signal value is a prerequisite for obtaining the accurate content of the substance to be tested.

[0003] To ensure that the instrument accurately analyzes the reaction signal, the reaction line should appear as a flat solid line (or rectangle) with clear edges and uniform interior. The background signal noise should be significantly different from the reaction line and appear as a uniform low signal state. However, the inhomogeneity of the solid phase carrier material, the complexity and diversity of the detection environment and the detection sample, and improper operation may lead to abnormal signals between the detection line and the detection background (such as Figure 4 Among them, common detection line signal abnormalities are local high fluorescence (also known as "reaction line bright spot") and local low signal (also known as "reaction line gap"), and common detection background signal abnormalities are local high signal (also known as "background bright spot").

[0004] In the prior art, the measurement accuracy is usually improved by repeatedly detecting the sample concentration. However, when the detection line and the detection background have signal abnormalities, repeated detection of the sample concentration cannot improve the measurement accuracy. Therefore, the existing sample concentration detection method has the problem of low detection accuracy. Summary of the invention

[0005] The embodiments of the present invention provide an immunochromatographic reagent card detection method, a storage medium and a system, which are intended to solve the problem of low detection accuracy in existing sample concentration detection methods.

[0006] In a first aspect, an embodiment of the present invention provides an immunochromatographic reagent card detection method, the method comprising:

[0007] An immunochromatographic analysis reagent card is provided, the immunochromatographic analysis reagent card comprising a test strip, the test strip comprising at least a sample pad, an NC membrane and a water absorbent pad, the sample pad is located upstream of the NC membrane, a reaction line is provided on the NC membrane, and the reaction line comprises a quality control line C and a detection line T;

[0008] Scanning the test strip to obtain a one-dimensional fluorescence signal curve, wherein the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine a first detection result of the sample to be tested;

[0009] Judging whether the reagent card has an abnormality based at least on the number of peaks of the characteristic peak of the one-dimensional fluorescence signal curve and / or the peak value of the characteristic peak and / or the area of ​​the characteristic peak and / or the half-height width of the characteristic peak;

[0010] When it is determined that there is no abnormality in the reagent card, the first test result is output; when it is determined that there is an abnormality in the reagent card, at least first abnormality prompt information is output and / or a test result confirmation operation is performed.

[0011] In a second aspect, an embodiment of the present invention further provides an immunochromatographic reagent card detection method, the method comprising:

[0012] An immunochromatographic analysis reagent card is provided, the immunochromatographic analysis reagent card comprising a test strip, the test strip comprising at least a sample pad, an NC membrane and a water absorbent pad, the sample pad is located upstream of the NC membrane, a reaction line is provided on the NC membrane, and the reaction line comprises a quality control line C and a detection line T;

[0013] Scanning the test strip to obtain a one-dimensional fluorescence signal curve, wherein the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine a first detection result of the sample to be tested;

[0014] Acquire the two-dimensional fluorescence signal image of the test strip, based on the two-dimensional fluorescence signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line area 2D-T , judge the I 2D-C and / or the I 2D-T Whether the signal uniformity meets the preset uniformity condition;

[0015] If yes, output the first detection result;

[0016] If not, a second abnormal prompt message is output and / or a fluorescence signal correction operation is performed.

[0017] In a third aspect, an embodiment of the present invention further provides an immunochromatographic reagent card detection method, the method comprising:

[0018] An immunochromatographic analysis reagent card is provided, the immunochromatographic analysis reagent card comprising a test strip, the test strip comprising at least a sample pad, an NC membrane and a water absorbent pad, the sample pad is located upstream of the NC membrane, a reaction line is provided on the NC membrane, and the reaction line comprises a quality control line C and a detection line T;

[0019] Acquire the two-dimensional fluorescence signal image of the test strip, based on the two-dimensional fluorescence signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line area 2D-T , judge the I 2D-C and / or the I 2D-T Whether the signal uniformity meets the preset uniformity condition;

[0020] If not, a second abnormal prompt message is output and / or a fluorescence signal correction operation is performed.

[0021] In a fourth aspect, an embodiment of the present invention further provides a computer-readable storage medium, wherein the storage medium includes a program, and the program can be executed by a processor to implement the method described in the first aspect and / or the second aspect and / or the third aspect above.

[0022] In a fifth aspect, an embodiment of the present invention further provides an immunochromatographic analysis system, the immunochromatographic analysis system comprising:

[0023] A signal detection module, used to obtain a one-dimensional fluorescence signal curve and / or a two-dimensional fluorescence signal image;

[0024] A processor is used to execute the program in the storage medium.

[0025] The present invention provides an immunochromatographic reagent card detection method, a storage medium and a system, the method comprising: providing an immunochromatographic analysis reagent card, the immunochromatographic analysis reagent card comprising a test strip, the test strip comprising at least a sample pad, an NC membrane and a water absorbent pad, the sample pad is located upstream of the NC membrane, a reaction line is provided on the NC membrane, the reaction line comprises a quality control line C and a detection line T; scanning the test strip to obtain a one-dimensional fluorescence signal curve, the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine a first detection result of a sample to be tested; judging whether the reagent card has an abnormality based on at least the number of peaks of a characteristic peak of the one-dimensional fluorescence signal curve and / or the peak value of the characteristic peak and / or the area of ​​the characteristic peak and / or the half-height width of the characteristic peak; when judging that the reagent card does not have an abnormality, outputting the first detection result; when judging that the reagent card has an abnormality, at least outputting first abnormality prompt information and / or performing a detection result confirmation operation. The embodiment of the present invention can judge whether there is an abnormality in the reagent card based on at least the number of peaks of the characteristic peak and / or the peak value of the characteristic peak and / or the area of ​​the characteristic peak and / or the half-height width of the characteristic peak of the one-dimensional fluorescence signal curve; when it is judged that there is no abnormality in the reagent card, the first detection result is output; when it is judged that there is an abnormality in the reagent card, at least the first abnormal prompt information is output and / or the detection result confirmation operation is performed; wherein, the relevant personnel are reminded by outputting the first abnormal prompt information so that the relevant personnel can understand the situation in time and take corresponding measures; the abnormal fluorescence signal is repaired by performing the detection result confirmation operation, thereby achieving the effect of correcting the bias of the detection result and improving the detection accuracy of the sample to be tested. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying any creative work.

[0027] Figure 1 A schematic diagram of a first process flow of an immunochromatographic reagent card detection method provided by an embodiment of the present invention;

[0028] Figure 2 A second schematic diagram of the immunochromatographic reagent card detection method provided by an embodiment of the present invention;

[0029] Figure 3 A third flow chart of the immunochromatographic reagent card detection method provided in an embodiment of the present invention;

[0030] Figure 4It is a schematic diagram of the abnormality of the detection line and the detection background signal in the prior art. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.

[0033] It should also be understood that the terms used in this specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. As used in the specification of the present invention and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.

[0034] It should be further understood that the term "and / or" used in the present description and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0035] See also Figure 1 , Figure 1 The present invention provides a flow chart of an immunochromatographic reagent card detection method according to an embodiment of the present invention. The method is applied to an information processing platform of an immunochromatographic analysis system. The reagent card detection and identification system includes a driving module, a mechanical module, a liquid circuit module, a signal detection module, an acquisition module, and a human-computer interaction and result display module; the driving module is used to control the liquid circuit module and the mechanical module according to a timing control instruction; wherein the timing control instruction comes from the information processing platform; the mechanical module is used to cooperate with the liquid circuit module to complete sample preparation, transportation, mixing, card feeding, incubation and other functions; the liquid circuit module is used to complete sample preparation, transportation, mixing, reaction, cleaning and other functions; the signal detection module is used to detect the signal through a fluorescence detection sensor and output the immunochromatographic analysis reagent card; the acquisition module is used to convert the detected signal into a digital signal and record the detection waveform; the information processing platform is responsible for the translation of the timing control instruction, interactive information processing and detection line and control line algorithm processing, etc.; the human-computer interaction and result display module is responsible for the interaction with the operator and the display of parameter results, etc.

[0036] Figure 1 The first flow chart of the immunochromatographic reagent card detection method provided by the embodiment of the present invention. Figure 1 As shown, the method includes the following steps S110-S140.

[0037] S110, providing an immunochromatographic analysis reagent card, wherein the immunochromatographic analysis reagent card comprises a test strip, wherein the test strip comprises at least a sample pad, an NC membrane and a water absorbent pad, wherein the sample pad is located upstream of the NC membrane, and a reaction line is provided on the NC membrane, wherein the reaction line comprises a quality control line C and a detection line T.

[0038] In this embodiment, an immunochromatographic analysis reagent card is provided, which includes a test strip, and the test strip includes at least a sample pad, an NC membrane and a water absorbent pad. The sample pad is located upstream of the NC membrane, and a reaction line is provided on the NC membrane. The reaction line includes a quality control line C and a detection line T. The detection result of the reagent card is presented through the reaction line.

[0039] S120, scanning the test strip to obtain a one-dimensional fluorescence signal curve, wherein the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine a first detection result of the sample to be tested.

[0040] In this embodiment, the signal detection module includes an APD camera, which is used to scan the test strip to obtain the one-dimensional fluorescence signal curve, and the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine the first detection result of the sample to be tested; specifically, the sample concentration of the sample to be tested can be determined based on the full peak area of ​​the detection curve corresponding to the detection line T in the one-dimensional fluorescence signal curve, and the sample concentration is used as the first detection result.

[0041] S130, judging whether the reagent card has an abnormality based at least on the number of peaks of the characteristic peak of the one-dimensional fluorescence signal curve and / or the peak value of the characteristic peak and / or the area of ​​the characteristic peak and / or the half-height width of the characteristic peak.

[0042] In this embodiment, when the peak data volume of the characteristic peak of the one-dimensional fluorescence signal curve is within a preset first range and / or the peak value of the characteristic peak is within a preset second range and / or the area of ​​the characteristic peak is within a preset third range and / or the ratio of the peak value to the area of ​​the characteristic peak is within a preset fourth range and / or the half-height width of the characteristic peak is within a preset fifth range and / or the ratio of the half-height width of the characteristic peak to the characteristic peak width is within a preset sixth range and / or the difference in the half-height width of any two reaction lines in the one-dimensional fluorescence signal curve is not greater than a preset first ratio threshold and / or the difference in the characteristic peak width of any two reaction lines in the one-dimensional fluorescence signal curve is not greater than a preset second ratio threshold, it is determined that there is no abnormality in the reagent card; wherein, the preset first range, the preset second range, the preset third range, the preset fourth range, the preset fifth range, the preset sixth range, the preset first ratio threshold, and the preset second ratio threshold can be set according to actual applications.

[0043] When the peak data volume of the characteristic peak of the one-dimensional fluorescence signal curve is not within the preset first range and / or the peak value of the characteristic peak is not within the preset second range and / or the area of ​​the characteristic peak is not within the preset third range and / or the ratio of the peak value to the area of ​​the characteristic peak is not within the preset fourth range and / or the half-height width of the characteristic peak is not within the preset fifth range and / or the ratio of the half-height width of the characteristic peak to the characteristic peak width is not within the preset sixth range and / or the difference in the half-height width of any two reaction lines in the one-dimensional fluorescence signal curve is greater than the preset first ratio threshold and / or the difference in the characteristic peak width of any two reaction lines in the one-dimensional fluorescence signal curve is greater than the preset second ratio threshold, it is determined that the reagent card is abnormal.

[0044] S140. When it is determined that there is no abnormality in the reagent card, the first test result is output; when it is determined that there is an abnormality in the reagent card, at least first abnormality prompt information is output and / or a test result confirmation operation is performed.

[0045] In this embodiment, when it is determined that there is no abnormality in the reagent card, the first test result is output; when it is determined that there is an abnormality in the reagent card, at least the first abnormality prompt information is output and / or the test result confirmation operation is performed. The present invention outputs the first abnormality prompt information to remind relevant personnel, and performs the test result confirmation operation to correct the bias of the test result, thereby improving the detection accuracy of the sample to be tested.

[0046] In one embodiment, the test result confirmation operation includes: obtaining a two-dimensional fluorescent signal image I of the test strip. 2D , the two-dimensional fluorescence signal image I 2D At least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; based on the two-dimensional fluorescence signal image I2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line T area 2D-T , judge the I 2D-C and / or the I 2D-T whether the signal uniformity meets the preset uniformity condition; if yes, output the first detection result; if not, output the second abnormal prompt information and / or perform the fluorescence signal correction operation.

[0047] In this embodiment, the signal detection module includes a CCD camera and / or a CMOS camera, and the CCD camera and / or the CMOS camera can be used to scan the test strip to obtain the two-dimensional fluorescence signal image I 2D , the two-dimensional fluorescence signal image I 2D At least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; based on the two-dimensional fluorescence signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line T area 2D-T , judge the I 2D-C and / or the I 2D-T whether the signal uniformity meets the preset uniformity condition; if yes, the first detection result is output; if not, the second abnormal prompt information is output and / or the fluorescence signal correction operation is performed; wherein, the second abnormal prompt information can be "reaction line gap abnormality" or "reaction line bright spot abnormality" or "reaction line fence abnormality" or "background bright spot abnormality".

[0048] Further, judging the I 2D-C and / or the I 2D-T whether the signal uniformity satisfies a preset uniformity condition; if yes, outputting the first detection result, and at least outputting the I 2D and / or the I 2D-C and / or the I 2D-T If not, then output the second abnormal prompt information and / or perform the fluorescence signal correction operation, and at least output the I 2D and / or the I 2D-C and / or the I 2D-T .

[0049] In one embodiment, the determining of the I 2D-C and / or the I 2D-T Whether the signal uniformity satisfies the preset uniformity condition includes: obtaining the I 2D-C And the I 2D-TThe number of non-zero pixel points in the non-zero pixel array is obtained, wherein the non-zero pixel array includes a plurality of row data; the non-zero pixel array is traversed to calculate the total number of row data equal to zero in the non-zero pixel array; it is determined whether the total number is less than 1 to obtain a row data total number determination result; if the row data determination result is yes, it is determined that I 2D-C and / or the I 2D-T The signal uniformity satisfies the preset uniformity condition; if the row data judgment result is no, it is determined that I 2D-C and / or the I 2D-T The signal uniformity does not meet the preset uniformity condition.

[0050] In this embodiment, the I is obtained by row. 2D-C And the I 2D-T The number of non-zero pixels in the array A is obtained. j ; Wherein, the non-zero pixel array includes a plurality of row data; Specifically, Among them, wi is the column number of the current reaction line area, is the width of the current reaction line, j is the row number of the current reaction line area, is the height of the current reaction line, p i is the gray value of the pixel, 0 <p i ≤255; for example, is the number of pixels in the first row whose grayscale value is 255 (i.e., the row data of the first row); the non-zero pixel array is traversed to calculate the total number of row data in the non-zero pixel array that is equal to zero, sum, j is the row number of the current reaction line area, is the height of the current reaction line, A j is the number of non-zero pixels in the current row; determining whether the total number is less than 1, and obtaining a row data total number determination result; if the row data determination result is yes, determining the I 2D-C and / or the I 2D-T The signal uniformity satisfies the preset uniformity condition; if the row data judgment result is no, it is determined that I 2D-C and / or the I 2D-T The signal uniformity does not meet the preset uniformity condition.

[0051] In one embodiment, the fluorescence signal correction operation includes: 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 , at least based on the I 2D-C1 and / or the I 2D-T1Determine a second test result of the sample to be tested, and output the second test result.

[0052] In this embodiment, the I 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 , at least based on the I 2D-C1 and / or the I 2D-T1 Determine a second test result of the sample to be tested, where the second test result is the concentration of the repaired sample; and output the second test result.

[0053] Further, the at least based on the I 2D-C1 and / or the I 2D-T1 After determining the second test result of the sample to be tested, the method further includes: outputting the second test result, and at least outputting the I 2D-C1 and / or the I 2D-T1 .

[0054] In one embodiment, the I 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 The method comprises: determining the type of outliers according to the non-zero pixel array; judging whether the image correction condition is met according to the type of outliers and the total number; if the condition is met, correcting the image according to the type of outliers and the total number. 2D-C and / or the I 2D-T Perform the corresponding image correction operation to obtain the corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 ; If not satisfied, execute the corresponding abnormal warning operation.

[0055] In this embodiment, the non-zero pixel array A is traversed. j If A j = 0 and j has a continuous period, then the abnormal point type is defined as "reaction line gap abnormality"; if A j = 0 and j has two consecutive segments, then the abnormal point type is defined as "reaction line bright spot abnormality"; if A j = 0 and j exists in multiple continuous segments, the abnormal point type is defined as "reaction line fence abnormality" or "background bright spot abnormality".

[0056] If the abnormal point type is "reaction line gap abnormality" and the total sum is less than or equal to the preset threshold in the image correction condition, it is determined that the image correction condition is met; wherein the preset threshold is a custom parameter;2D-C and / or the I 2D-T The corresponding image correction operation is performed: take the center point as the symmetry point, find the fluorescence signal intensity of the signal missing area and the signal non-missing area that is symmetrical to the center. If the signal non-missing area that is symmetrical to the center is also a signal missing area, and the signal missing area is close to the center area, then the sampling is diffused to both sides; if the signal missing area is close to the two side areas, then the sampling is diffused to the center.

[0057] Further, after the restoration is completed, at least based on the corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 The sample concentration of the sample to be tested is determined and used as the second detection result.

[0058] If the abnormal point type is "reaction line gap abnormality" and the total sum is greater than the preset threshold in the image correction condition, it is determined that the image correction condition is not met. At this time, an abnormal alarm prompt (i.e., abnormal warning operation) is required to suggest the user to replace the reagent card and retest.

[0059] If the abnormal point type is "reaction line bright spot abnormality" and the total sum is less than or equal to the preset threshold in the image correction condition, it is determined that the image correction condition is met. At this time, the I 2D-C and / or the I 2D-T Perform the corresponding image correction operation to obtain the corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 The image correction operation is: take the center point as the symmetry point, find the fluorescence signal intensity of the signal missing area and the signal non-missing area symmetrical to the center. If the signal non-missing area symmetrical to the center is also a signal missing area, and the signal missing area is close to the center area, then diffuse sampling to both sides; if the signal missing area is close to the two side areas, then diffuse sampling to the center.

[0060] Further, after the restoration is completed, at least based on the corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 The sample concentration of the sample to be tested is determined and used as the second detection result.

[0061] If the abnormal point type is "reaction line bright spot abnormality" and the total number sum is greater than the preset threshold in the image correction condition, it is determined that the image correction condition is not met. At this time, an abnormal alarm prompt (i.e., abnormal warning operation) is required to suggest the user to replace the reagent card and retest.

[0062] If the abnormal point type is "reaction line fence abnormality" or "background bright spot abnormality", it is determined that the image correction condition is not met. At this time, an abnormal alarm prompt (i.e., abnormal warning operation) is required to suggest the user to replace the reagent card and re-test.

[0063] In one embodiment, the test result confirmation operation includes: obtaining a two-dimensional fluorescent signal image I of the test strip 2D , output the two-dimensional fluorescence signal image I 2D and / or based on the two-dimensional fluorescence signal image I 2D The converted three-dimensional fluorescence signal image I 3D , the two-dimensional fluorescence signal image I 2D and / or the three-dimensional fluorescence signal image I 3D Used to confirm whether there is any abnormality in the reagent card.

[0064] In this embodiment, the test result confirmation operation includes: obtaining a two-dimensional fluorescent signal image I of the test strip. 2D , output the two-dimensional fluorescence signal image I 2D and / or based on the two-dimensional fluorescence signal image I 2D The converted three-dimensional fluorescence signal image I 3D In order to improve the detection rate of abnormal points of the reaction line, the two-dimensional fluorescence signal image I 2D and / or the three-dimensional fluorescence signal image I 3D Used to confirm whether there is any abnormality in the reagent card.

[0065] Specifically, based on the three-dimensional fluorescence signal image I 3D Obtain a three-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 3D-C And the three-dimensional fluorescence signal image I of the detection line T area 3D-T , judge the I 3D-C and / or the I 3D-T whether the signal uniformity meets the preset uniformity condition; if yes, output the first detection result; if not, output the second abnormal prompt information and / or perform the fluorescence signal correction operation.

[0066] In one embodiment, the test result confirmation operation includes: 2DPerform non-deformation normalization to obtain a normalized reagent card image; crop the normalized reagent card image according to a preset cropping area parameter to obtain a reagent card cropped image; perform RGB channel separation on the reagent card cropped image to obtain a single-channel reagent card image group; add the R channel reagent card image in the single-channel reagent card image group to the grayscale image of the reagent card cropped image to obtain a reagent card enhanced image; perform reaction line extraction on the reagent card enhanced image to obtain a quality control image corresponding to the quality control line C area and a detection image corresponding to the detection line T area; copy the quality control image and the detection image to the first area and the second area in the preset average grayscale image respectively to obtain an initial preprocessed reagent card image; binarize the initial preprocessed reagent card image to obtain a three-dimensional fluorescence signal image I 3D , the three-dimensional fluorescence signal image I 3D Used to confirm whether there is any abnormality in the reagent card.

[0067] In this embodiment, the two-dimensional fluorescence signal image I of the test strip 2D Perform non-deformation normalization to obtain a normalized reagent card image; specifically, set the image normalization size to (w, h), w is the width value, h is the height value; according to the I 2D The size (iw, ih) of the X direction is calculated by scaling factor X scale and Y direction scaling factor Y scale , where X scale =iw / w,Y scale =ih / h, and the width and height of the reagent card image are multiplied by the corresponding scaling coefficients to obtain a normalized reagent card image.

[0068] According to the preset clipping area parameters [X ROI , Y ROI , W ROI , H ROI ] The normalized reagent card image is cropped to obtain a reagent card cropped image; wherein, X ROI is the horizontal coordinate of the clipping area, Y ROI is the ordinate of the clipping area, W ROI is the width of the clipping area, H ROI The height of the clipping area.

[0069] The RGB channels of the reagent card cropped image are separated to obtain a single-channel reagent card image group; the R channel reagent card image in the single-channel reagent card image group is added to the grayscale image of the reagent card cropped image to obtain a reagent card enhanced image; the embodiment of the present invention enhances the color development degree of the reaction line in the reagent card enhanced image by adding the R channel reagent card image in the single-channel reagent card image group to the grayscale image of the reagent card cropped image.

[0070] Reaction line extraction is performed on the reagent card enhanced image to obtain a quality control image corresponding to the quality control line C area and a detection image corresponding to the detection line T area; the quality control image and the detection image are respectively copied to the first area and the second area in the preset average grayscale image to obtain an initial pre-processed reagent card image; the initial pre-processed reagent card image is binarized to obtain a three-dimensional fluorescence signal image I 3D , the three-dimensional fluorescence signal image I 3D Used to confirm whether there is an abnormality in the reagent card; specifically, the image binarization method such as fixed threshold method, average method, bimodal method, OTSU method, etc. can be used to binarize the initial pre-processed reagent card image.

[0071] In one embodiment, the single-channel reagent card image group includes multiple single-channel reagent card images. After the RGB channel separation of the reagent card cropped image is performed to obtain the single-channel reagent card image group, it also includes: binarizing each of the single-channel reagent card images to obtain a corresponding binarized single-channel reagent card image; performing a maximum contour search on each of the binarized single-channel reagent card images to obtain a corresponding contour area value; obtaining a maximum contour area value from each of the contour area values; determining whether the maximum contour area value is less than a preset fixed threshold to obtain a second determination result; if the second determination result is no, performing the step of adding the R channel reagent card image to the grayscale image of the reagent card cropped image to obtain a reagent card enhanced image.

[0072] In this embodiment, the single-channel reagent card image group includes an R single-channel reagent card image, a G single-channel reagent card image and a B single-channel reagent card image, and each of the single-channel reagent card images is binarized to obtain a corresponding binarized single-channel reagent card image; a maximum contour search is performed on each of the binarized single-channel reagent card images to obtain a corresponding contour area value; the maximum contour area value is obtained from each of the contour area values; it is determined whether the maximum contour area value is less than a preset fixed threshold value to obtain a second judgment result; if the second judgment result is no, the step of adding the R channel reagent card image to the grayscale image of the reagent card cropped image to obtain a reagent card enhanced image is executed; if the second judgment result is yes, the reagent card is determined to be a blank card; when the second judgment result is yes, a prompt message indicating that the reagent card is a blank card is output on the terminal interface.

[0073] In one embodiment, the quality control image and the test image are respectively copied to the first area and the second area in a preset average grayscale image to obtain an initial pre-processed reagent card image, including: obtaining the average grayscale value of the non-reaction line area of ​​the reagent card enhanced image; creating the average grayscale image according to the average grayscale value; and the quality control image and the test image are respectively copied to the first area and the second area in the average grayscale image to obtain an initial pre-processed reagent card image.

[0074] In this embodiment, the average gray value of the non-reaction line area of ​​the reagent card enhanced image is obtained; the average gray image is created according to the average gray value; wherein the length and width of the average gray image are consistent with the length and width of the window of the reagent card; the quality control image and the test image are respectively copied to the first area and the second area in the average gray image to obtain the initial pre-processed reagent card image. The present invention can eliminate the noise interference problem in the non-reaction line area by creating the average gray image, thereby improving the detection rate of abnormal points of the reaction line.

[0075] The embodiment of the present invention can judge whether there is an abnormality in the reagent card based on at least the number of peaks of the characteristic peak and / or the peak value of the characteristic peak and / or the area of ​​the characteristic peak and / or the half-height width of the characteristic peak of the one-dimensional fluorescence signal curve; when it is judged that there is no abnormality in the reagent card, the first detection result is output; when it is judged that there is an abnormality in the reagent card, at least the first abnormal prompt information is output and / or the detection result confirmation operation is performed; wherein, the relevant personnel are reminded by outputting the first abnormal prompt information so that the relevant personnel can understand the situation in time and take corresponding measures; the abnormal fluorescence signal is repaired by performing the detection result confirmation operation, thereby achieving the effect of correcting the bias of the detection result and improving the detection accuracy of the sample to be tested.

[0076] Figure 2 The second flow chart of the immunochromatographic reagent card detection method provided in the embodiment of the present invention. Figure 2 As shown, the method includes the following steps S210-S240.

[0077] S210, providing an immunochromatographic analysis reagent card, wherein the immunochromatographic analysis reagent card comprises a test strip, wherein the test strip comprises at least a sample pad, an NC membrane and a water absorbent pad, wherein the sample pad is located upstream of the NC membrane, and a reaction line is provided on the NC membrane, wherein the reaction line comprises a quality control line C and a detection line T;

[0078] S220, scanning the test strip to obtain a one-dimensional fluorescence signal curve, wherein the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine a first detection result of the sample to be tested;

[0079] S230, obtaining a two-dimensional fluorescence signal image of the test strip, based on the two-dimensional fluorescence signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line area 2D-T , judge the I 2D-C and / or the I 2D-T Whether the signal uniformity meets the preset uniformity condition;

[0080] S240: If yes, output the first detection result; if no, output second abnormality prompt information and / or perform a fluorescence signal correction operation.

[0081] In the present embodiment, an immunochromatographic analysis reagent card is provided, wherein the immunochromatographic analysis reagent card comprises a test strip, wherein the test strip comprises at least a sample pad, an NC membrane and a water absorbent pad, wherein the sample pad is located upstream of the NC membrane, and a reaction line is provided on the NC membrane, wherein the reaction line comprises a quality control line C and a detection line T; the test strip is scanned to obtain a one-dimensional fluorescence signal curve, wherein the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine a first detection result of a sample to be tested; and at the same time, a two-dimensional fluorescence signal image of the test strip is obtained, and based on the two-dimensional fluorescence signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line area 2D-T , judge the I 2D-C and / or the I 2D-T whether the signal uniformity meets the preset uniformity condition; if yes, output the first detection result; if not, output the second abnormal prompt information and / or perform the fluorescence signal correction operation.

[0082] The embodiments of the present invention can combine signal detection technology and image processing technology to improve the detection accuracy of the sample to be tested.

[0083] In one embodiment, the fluorescence signal correction operation includes: 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 , at least based on the I 2D-C1 and / or the I 2D-T1 Determine a second test result of the sample to be tested, and output the second test result.

[0084] In this embodiment, the I 2D-C and / or the I2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 , at least based on the I 2D-C1 and / or the I 2D-T1 Determine a second test result of the sample to be tested, where the second test result is the concentration of the repaired sample; and output the second test result.

[0085] Further, the at least based on the I 2D-C1 and / or the I 2D-T1 After determining the second test result of the sample to be tested, the method further includes: outputting the second test result, and at least outputting the I 2D-C1 and / or the I 2D-T1 .

[0086] Figure 3 The third flow chart of the immunochromatographic reagent card detection method provided in the embodiment of the present invention is as follows. Figure 3 As shown, the method includes the following steps S310-S330.

[0087] S310, providing an immunochromatographic analysis reagent card, wherein the immunochromatographic analysis reagent card comprises a test strip, wherein the test strip comprises at least a sample pad, an NC membrane and a water absorbent pad, wherein the sample pad is located upstream of the NC membrane, and a reaction line is provided on the NC membrane, wherein the reaction line comprises a quality control line C and a detection line T;

[0088] S320, obtaining a two-dimensional fluorescence signal image of the test strip, based on the two-dimensional fluorescence signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line area 2D-T , judge the I 2D-C and / or the I 2D-T Whether the signal uniformity meets the preset uniformity condition;

[0089] S330: If not, output a second abnormal prompt message and / or perform a fluorescence signal correction operation.

[0090] In this embodiment, an immunochromatographic analysis reagent card is provided, wherein the immunochromatographic analysis reagent card comprises a test strip, wherein the test strip comprises at least a sample pad, an NC membrane and a water absorbent pad, wherein the sample pad is located upstream of the NC membrane, and a reaction line is provided on the NC membrane, wherein the reaction line comprises a quality control line C and a detection line T; a two-dimensional fluorescent signal image of the test strip is obtained, and based on the two-dimensional fluorescent signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line area2D-T , judge the I 2D-C and / or the I 2D-T whether the signal uniformity meets the preset uniformity condition; if not, outputting the second abnormal prompt information and / or performing the fluorescence signal correction operation; if yes, scanning the test strip to obtain a one-dimensional fluorescence signal curve, the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine the first detection result of the sample to be tested; and outputting the first detection result.

[0091] In one embodiment, the fluorescence signal correction operation includes: 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 , at least based on the I 2D-C1 and / or the I 2D-T1 Determine a second test result of the sample to be tested, and output the second test result.

[0092] In this embodiment, the I 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 , at least based on the I 2D-C1 and / or the I 2D-T1 Determine a second test result of the sample to be tested, where the second test result is the concentration of the repaired sample; and output the second test result.

[0093] Further, the at least based on the I 2D-C1 and / or the I 2D-T1 After determining the second test result of the sample to be tested, the method further includes: outputting the second test result, and at least outputting the I 2D-C1 and / or the I 2D-T1 .

[0094] The present invention also provides a computer-readable storage medium, which includes a program, and the program can be executed by a processor to implement the above-mentioned immunochromatographic reagent card detection method; wherein the storage medium can be a U disk, a mobile hard disk, a read-only memory (ROM), a magnetic disk or an optical disk, etc., which can store program codes. Computer-readable storage media.

[0095] The present invention also provides an immunochromatographic analysis system, which includes: a signal detection module for acquiring a one-dimensional fluorescence signal curve and / or a two-dimensional fluorescence signal image; and a processor for executing a program in the storage medium.

[0096] Those of ordinary skill in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, computer software, or a combination of the two. In order to clearly illustrate the interchangeability of hardware and software, the composition and steps of each example have been generally described in terms of function in the above description. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of the present invention.

[0097] In the several embodiments provided by the present invention, it should be understood that the disclosed devices and methods can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of each unit is only a logical function division, and there may be other division methods in actual implementation. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed.

[0098] The steps in the method of the embodiment of the present invention can be adjusted in order, combined and deleted according to actual needs. The units in the device of the embodiment of the present invention can be combined, divided and deleted according to actual needs. In addition, the functional units in the various embodiments of the present invention can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit.

[0099] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a storage medium. Based on this understanding, the technical solution of the present invention is essentially or the part that contributes to the prior art, or all or part of the technical solution can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including a number of instructions for an electronic device to perform all or part of the steps of the method described in each embodiment of the present invention.

[0100] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the present invention, and these modifications or replacements should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. An immunochromatographic reagent card detection method, characterized in that: The method comprises: An immunochromatographic analysis reagent card is provided, the immunochromatographic analysis reagent card comprising a test strip, the test strip comprising at least a sample pad, an NC membrane and a water absorbent pad, the sample pad is located upstream of the NC membrane, a reaction line is provided on the NC membrane, and the reaction line comprises a quality control line C and a detection line T; Scanning the test strip to obtain a one-dimensional fluorescence signal curve, wherein the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine a first detection result of the sample to be tested; Judging whether the reagent card has an abnormality based at least on the number of peaks of the characteristic peak of the one-dimensional fluorescence signal curve and / or the peak value of the characteristic peak and / or the area of ​​the characteristic peak and / or the half-height width of the characteristic peak; When it is determined that there is no abnormality in the reagent card, the first test result is output; when it is determined that there is an abnormality in the reagent card, at least first abnormality prompt information is output and / or a test result confirmation operation is performed.

2. The immunochromatographic reagent card detection method according to claim 1, characterized in that: The step of judging whether the reagent card is abnormal based on at least the number of peaks of the characteristic peak of the one-dimensional fluorescence signal curve and / or the peak value of the characteristic peak and / or the area of ​​the characteristic peak and / or the half-height width of the characteristic peak comprises: When the peak data volume of the characteristic peak of the one-dimensional fluorescence signal curve is within a preset first range and / or the peak value of the characteristic peak is within a preset second range and / or the area of ​​the characteristic peak is within a preset third range and / or the ratio of the peak value to the area of ​​the characteristic peak is within a preset fourth range and / or the half-height width of the characteristic peak is within a preset fifth range and / or the ratio of the half-height width of the characteristic peak to the characteristic peak width is within a preset sixth range and / or the difference value of the half-height width of any two reaction lines in the one-dimensional fluorescence signal curve is not greater than a preset first ratio threshold and / or the difference value of the characteristic peak width of any two reaction lines in the one-dimensional fluorescence signal curve is not greater than a preset second ratio threshold, it is determined that the reagent card does not have an abnormality; When the peak data volume of the characteristic peak of the one-dimensional fluorescence signal curve is not within the preset first range and / or the peak value of the characteristic peak is not within the preset second range and / or the area of ​​the characteristic peak is not within the preset third range and / or the ratio of the peak value to the area of ​​the characteristic peak is not within the preset fourth range and / or the half-height width of the characteristic peak is not within the preset fifth range and / or the ratio of the half-height width of the characteristic peak to the characteristic peak width is not within the preset sixth range and / or the difference in the half-height width of any two reaction lines in the one-dimensional fluorescence signal curve is greater than the preset first ratio threshold and / or the difference in the characteristic peak width of any two reaction lines in the one-dimensional fluorescence signal curve is greater than the preset second ratio threshold, it is determined that the reagent card is abnormal.

3. The immunochromatographic reagent card detection method according to claim 1, characterized in that: The test result confirmation operation includes: Obtaining a two-dimensional fluorescence signal image I of the test strip 2D , the two-dimensional fluorescence signal image I 2D At least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; Based on the two-dimensional fluorescence signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line T area 2D-T , judge the I 2D-C and / or the I 2D-T Whether the signal uniformity meets the preset uniformity condition; If yes, output the first detection result; If not, a second abnormal prompt message is output and / or a fluorescence signal correction operation is performed.

4. The immunochromatographic reagent card detection method according to claim 3, characterized in that: The judgment 2D-C and / or the I 2D-T Whether the signal uniformity meets the preset uniformity conditions includes: Get the I by row 2D-C And the I 2D-T The number of non-zero pixel points in the matrix is ​​obtained to obtain a non-zero pixel point array; wherein the non-zero pixel point array includes a plurality of row data; Traversing the non-zero pixel array, and calculating the total number of row data in the non-zero pixel array that are equal to zero; Determine whether the total number is less than 1, and obtain a determination result of the total number of row data; If the row data judgment result is yes, determine the I 2D-C and / or the I 2D-T The signal uniformity meets the preset uniformity condition; If the row data judgment result is no, determine the I 2D-C and / or the I 2D-T The signal uniformity does not meet the preset uniformity condition.

5. The immunochromatographic reagent card detection method according to claim 4, characterized in that: The fluorescence signal correction operation includes: The I 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 , at least based on the I 2D-C1 and / or the I 2D-T1 Determine a second test result of the sample to be tested, and output the second test result.

6. The immunochromatographic reagent card detection method according to claim 5, characterized in that: The I 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 include: Determine the type of abnormal point according to the non-zero pixel array; Determining whether an image correction condition is met according to the type of the abnormal points and the total number; If satisfied, the I 2D-C and / or the I 2D-T Perform the corresponding image correction operation to obtain the corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 ; If not satisfied, execute the corresponding abnormal warning operation.

7. The immunochromatographic reagent card detection method according to claim 5, characterized in that: The at least based on the I 2D-C1 and / or the I 2D-T1 After determining the second test result of the sample to be tested, the method further includes: Output the second detection result, and at least output the I 2D-C1 and / or the I 2D-T1 .

8. The immunochromatographic reagent card detection method according to claim 3, characterized in that: Judging the I 2D-C and / or the I 2D-T Whether the signal uniformity meets the preset uniformity condition; If yes, then the first detection result is output, and at least the I 2D and / or the I 2D-C and / or the I 2D-T ; If not, output the second abnormal prompt information and / or perform the fluorescence signal correction operation, and at least output the I 2D and / or the I 2D-C and / or the I 2D-T .

9. The immunochromatographic reagent card detection method according to claim 1, characterized in that: The test result confirmation operation includes: Obtaining a two-dimensional fluorescence signal image I of the test strip 2D , output the two-dimensional fluorescence signal image I 2D and / or based on the two-dimensional fluorescence signal image I 2D The converted three-dimensional fluorescence signal image I 3D , the two-dimensional fluorescence signal image I 2D and / or the three-dimensional fluorescence signal image I 3D Used to confirm whether there is any abnormality in the reagent card.

10. The immunochromatographic reagent card detection method according to claim 1, characterized in that: The test result confirmation operation includes: The two-dimensional fluorescence signal image I of the test strip 2D Perform non-deformation normalization to obtain a normalized reagent card image; Cropping the normalized reagent card image according to preset cropping area parameters to obtain a reagent card cropped image; Separating the RGB channels of the reagent card cropped image to obtain a single-channel reagent card image group; Adding the R channel reagent card image in the single channel reagent card image group to the grayscale image of the reagent card cropped image to obtain a reagent card enhanced image; Extracting reaction lines from the enhanced image of the reagent card to obtain a quality control image corresponding to the quality control line C area and a detection image corresponding to the detection line T area; Copying the quality control image and the detection image to the first area and the second area in the preset average grayscale image respectively to obtain an initial preprocessed reagent card image; Binarize the initial preprocessed reagent card image to obtain a three-dimensional fluorescence signal image I 3D , the three-dimensional fluorescence signal image I 3D Used to confirm whether there is any abnormality in the reagent card.

11. The immunochromatographic reagent card detection method according to claim 10, characterized in that: The single-channel reagent card image group includes a plurality of single-channel reagent card images, and after the reagent card cropped image is separated into RGB channels to obtain the single-channel reagent card image group, the method further includes: Binarizing each of the single-channel reagent card images to obtain a corresponding binary single-channel reagent card image; Performing maximum contour search on each of the binary single-channel reagent card images to obtain a corresponding contour area value; Obtaining a maximum contour area value from each of the contour area values; Determine whether the maximum contour area value is less than a preset fixed threshold value to obtain a second determination result; If the second judgment result is no, the step of adding the R channel reagent card image to the grayscale image of the reagent card cropped image to obtain a reagent card enhanced image is performed.

12. The immunochromatographic reagent card detection method according to claim 10, characterized in that: The step of copying the quality control image and the detection image to a first area and a second area in a preset average grayscale image to obtain an initial preprocessed reagent card image includes: Obtaining the average gray value of the non-reaction line area of ​​the reagent card enhanced image; Creating the average grayscale image according to the average grayscale value; The quality control image and the detection image are copied to the first area and the second area in the average grayscale image respectively to obtain an initial pre-processed reagent card image.

13. An immunochromatographic reagent card detection method, characterized in that: The method comprises: An immunochromatographic analysis reagent card is provided, the immunochromatographic analysis reagent card comprising a test strip, the test strip comprising at least a sample pad, an NC membrane and a water absorbent pad, the sample pad is located upstream of the NC membrane, a reaction line is provided on the NC membrane, and the reaction line comprises a quality control line C and a detection line T; Scanning the test strip to obtain a one-dimensional fluorescence signal curve, wherein the one-dimensional fluorescence signal curve is at least used to characterize the fluorescence signal of the quality control line C and the fluorescence signal of the detection line T; the fluorescence signal of the detection line T is at least used to determine a first detection result of the sample to be tested; Acquire the two-dimensional fluorescence signal image of the test strip, based on the two-dimensional fluorescence signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line area 2D-T , judge the I 2D-C and / or the I 2D-T Whether the signal uniformity meets the preset uniformity condition; If yes, output the first detection result; If not, a second abnormal prompt message is output and / or a fluorescence signal correction operation is performed.

14. The immunochromatographic reagent card detection method according to claim 13, characterized in that: The fluorescence signal correction operation includes: The I 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 , at least based on the I 2D-C1 and / or the I 2D-T1 Determine a second test result of the sample to be tested, and output the second test result.

15. An immunochromatographic reagent card detection method, characterized in that: The method comprises: An immunochromatographic analysis reagent card is provided, the immunochromatographic analysis reagent card comprising a test strip, the test strip comprising at least a sample pad, an NC membrane and a water absorbent pad, the sample pad is located upstream of the NC membrane, a reaction line is provided on the NC membrane, and the reaction line comprises a quality control line C and a detection line T; Acquire the two-dimensional fluorescence signal image of the test strip, based on the two-dimensional fluorescence signal image I 2D Obtain a two-dimensional fluorescence signal image I of the quality control line C area of ​​the test strip 2D-C And the two-dimensional fluorescence signal image I of the detection line area 2D-T , judge the I 2D-C and / or the I 2D-T Whether the signal uniformity meets the preset uniformity condition; If not, a second abnormal prompt message is output and / or a fluorescence signal correction operation is performed.

16. The immunochromatographic reagent card detection method according to claim 15, characterized in that: The fluorescence signal correction operation includes: The I 2D-C and / or the I 2D-T Perform image correction processing to obtain a corrected two-dimensional fluorescence signal image I 2D-C1 and / or 2D-T1 , at least based on the I 2D-C1 and / or the I 2D-T1 Determine a second test result of the sample to be tested, and output the second test result.

17. A computer-readable storage medium, characterized in that: The storage medium includes a program, and the program can be executed by a processor to implement the immunochromatographic reagent card detection method according to any one of claims 1 to 16.

18. An immunochromatographic analysis system, characterized in that: The immunochromatographic analysis system comprises: A signal detection module, used to obtain a one-dimensional fluorescence signal curve and / or a two-dimensional fluorescence signal image; A processor for executing a program in a computer-readable storage medium as claimed in claim 17.

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