Controllable thermal pain stimulation device applied to rats

Abstract

The invention belongs to the technical field of biological experiment devices, and particularly relates to a controllable thermal pain stimulation device applied to rats, which comprises a control unit used for performing a thermal pain stimulation experiment according to preset threshold control; the thermal stimulation module is used for automatically realizing a thermal pain stimulation experiment according to an instruction of the control unit and feeding back experimental data; and the power module is used for supplying power to the control unit. The thermal pain stimulation can be automatically carried out after parameters are preset, the operation is simple and convenient, human intervention is not needed, the preparation process, the working process and the ending process of the device are convenient and simple, and the device is clear in overall structure and has a flexible change space. On the premise that a user can set the temperature, the duration, the period, the number of times and the like within a flexible range, the device can be prevented from blocking the space of other collecting equipment and fixing equipment, effective thermal pain stimulation on the skin of the rat is achieved, and the device is suitable for thermal pain stimulation under many experimental operation conditions.

Description

Technical Field

[0001] The invention belongs to the technical field of biological experimental devices, and in particular relates to a controllable thermal pain stimulation device applied to rats. Background Art

[0002] The pain assessment method of rats is particularly important in basic research on pain. In the exploration of the physiological basis of pain and the evaluation of the analgesia level of clinical patients, researchers usually first use rats as experimental subjects, apply corresponding pain stimulation to evaluate pain perception or induce certain physiological reactions, such as autonomic nerve activity response, to explore the mechanism of pain level generation and the intensity of perception.

[0003] Pain is the sensation produced when an organism is subjected to harmful stimulation. Thermal pain stimulation can induce the body's pain perception, fluctuations in autonomic nervous activity, sudden somatic reactions, negative emotional reactions, and other phenomena. When the pain reaches a certain intensity, corresponding defensive behaviors may also occur. Due to the unique subjective emotional response characteristics of pain, in clinical practice, the assessment and quantification of pain is usually based on the patient's subjective score. However, rodents cannot express pain in words, so some specific assessment methods are often used, such as electrical stimulation, thermal radiation stimulation, and hot plate stimulation to analyze tail-wagging reactions and gait analysis. The current intensity parameters of electrical stimulation, the temperature parameters of thermal stimulation, the duration of stimulation, etc. are adjusted, and the rats' stress behaviors such as leg retraction and tail-wagging are observed to determine whether the rats feel pain or not.

[0004] There are corresponding detection instruments on the market for detecting thermal pain behavior in rodents. They are highly integrated and most of them have metal cages or transparent boxes. They are not suitable for fixing sensors for collecting signals such as the ECG, pulse wave, and respiration of rats in an open environment, nor for fixing gas anesthesia devices. The stimulation position of specific detection instruments is fixed and the stimulation site cannot be adjusted, which is also an obstacle for researchers to flexibly apply stimulation. At the same time, the changes in the indicators of thermal pain stimulation are not flexible and stable enough. The temperature accuracy is relatively large, the temperature change is not rapid during short-term thermal stimulation, the contact surface pressure is unknown, the stimulation duration and stimulation cycle are not automatically controlled, and the thermal pain stimulation caused is not completely automatic, but requires human intervention control and operation to ensure the orderly progress of the experiment. This leads to cumbersome and imprecise operations in physiological exploration experiments or application experiments on thermal pain stimulation in rats, and cannot guarantee effective and stable thermal pain stimulation on rat skin. Summary of the invention

[0005] The purpose of the present invention is to provide a controllable thermal pain stimulation device for rats, so as to solve the technical problems in the prior art that physiological exploration experiments or application experiments on thermal pain stimulation in rats are cumbersome and not rigorous in operation, and cannot ensure effective and stable thermal pain stimulation to rat skin.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: A controllable thermal pain stimulation device for rats, comprising: A control unit, used for controlling a thermal pain stimulation experiment according to a preset threshold value; The thermal stimulation module is used to automatically implement the thermal pain stimulation experiment according to the instructions of the control unit and feedback the experimental data; The power module is used to supply power to the control unit.

[0007] Preferably, the thermal stimulation module comprises: a shell, the shell is detachably arranged on a workbench, a fixed elastic band is arranged in the bottom of the shell, a stepper motor is arranged on the top of the shell, the stepper motor is connected to a control unit, the output end of the stepper motor passes through the shell and is connected to a ceramic heating plate, a temperature sensor and a pressure sensor are arranged on the ceramic heating plate, and the temperature sensor, the ceramic heating plate and the pressure sensor are all electrically connected to the control unit.

[0008] Preferably, a side hole is opened on the side wall of the shell, and the temperature sensor, the ceramic heating plate and the pressure sensor are all electrically connected to the control unit through the side hole.

[0009] Preferably, the shell is a frame structure that penetrates from front to back, and a padding cloth is provided on the inner bottom thereof, and the padding cloth is located below the fixed elastic band.

[0010] Preferably, a suction cup is arranged outside the bottom of the shell for fixing the shell on a workbench.

[0011] Preferably, the temperature sensor is arranged on the upper surface of the ceramic heating plate, and the pressure sensor is arranged on the lower surface of the ceramic heating plate.

[0012] Preferably, the control unit comprises: Arduino control module, used to receive pressure feedback signals and output control signals to the drive module; A driving module, used for adjusting the thermal stimulation module according to the control signal; a temperature control module, for receiving temperature feedback and outputting temperature control to the thermal stimulation module; The pressure control module is used to receive the pressure feedback from the thermal stimulation module and transmit the pressure feedback to the Arduino control module.

[0013] Preferably, the preset threshold value includes the thermal pain stimulation duration, cycle parameters, preset number of cycles and contact pressure threshold parameters set on the Arduino control module.

[0014] Preferably, the thermal stimulation module comprises: a stepper motor, a temperature sensor, a ceramic heating plate and a pressure sensor, the stepper motor is connected to the ceramic heating plate, and the temperature sensor and the pressure sensor are arranged on the ceramic heating plate; The driving module is connected to the stepping motor and is used to control the stepping direction and stepping speed of the stepping motor; The temperature control module is connected to the temperature sensor and the ceramic heating plate, and is used to output temperature control to the ceramic heating plate according to the temperature feedback received from the temperature sensor; The pressure control module is connected to the pressure sensor to receive pressure feedback transmitted by the pressure sensor and transmit the pressure feedback to the Arduino control module.

[0015] Preferably, the driving module is connected to the stepper motor via a stepper motor control connection line.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1) This application uses a control unit in conjunction with a thermal stimulation module to automatically implement thermal pain stimulation experiments, avoiding human intervention in control and operation, and improving the rigor and stability of the experiment; this application can automatically perform thermal pain stimulation after pre-setting parameters, and is easy to operate and does not require human intervention. The preparation process, working process, and ending process of the device are relatively convenient and simple. The overall structure of the device is clear and has more flexible room for change. The device can avoid spatial obstruction of other collection equipment and fixed equipment while satisfying the user's flexible setting of temperature, duration, cycle, number of times, etc., and can achieve effective thermal pain stimulation on rat skin, which is suitable for thermal pain stimulation in many experimental operation situations.

[0017] 2) The thermal stimulation module provided in this application is a stimulation device for an open environment. The shell is a hollow structure at the front and back. The shell can be detachably set on the workbench. When the rat needs to wear sensors for physiological signal collection, fix the gas anesthesia device or other contact operations, this thermal stimulation device can fix the shell at any position beside the rat to avoid complex fixing methods that make other equipment unable to be fixed on the rat. The fixed elastic band fixes the rat's foot or tail to the stable stimulation site of the ceramic heating plate, and aligns the pre-stimulated skin area of ​​the rat according to the mark on the device.

[0018] 3) The suction cup at the bottom of the shell realizes a detachable connection between the shell and the workbench. The device is stabilized on the desktop by squeezing downwards. It is convenient and quick to fix and easy to disassemble.

[0019] 4) The temperature of rat stimulation needs to be set in advance with an accuracy of 0.1°C. The temperature control module uses a temperature sensor placed above the ceramic heating plate to collect the temperature of the ceramic heating plate in real time. By comparing the difference with the ideal temperature, the power supply voltage of the heating plate is controlled to stabilize the heating plate within the expected range, avoiding differences in rat thermal pain stimulation responses caused by temperature disturbances.

[0020] 5) The thermal stimulation device uses the principle of stepper motor expansion and contraction to perform thermal pain stimulation. Generally, the thermal pain stimulation device uses the power supply of the thermal element to achieve temperature rise and fall to control the stimulation duration and determine the stimulation effect. It is easy to cause the heat accumulation of the heat source to affect the next continuous thermal pain stimulation. The temperature rise and fall rate is not constant and not timely, which affects the effect of pain assessment. For this reason, the present application fixes the thermal element at the end of the stepper motor, that is, the ceramic heating plate. The Arduino control module controls the stepper motor to extend and retract to achieve thermal pain stimulation of the thermal element on the rat skin, avoiding invalid pain stimulation caused by the thermal element not being able to rise and fall in time. At the same time, it can also flexibly adjust and accurately control the stimulation duration, cycle period and number of cycles of thermal pain stimulation. When the ceramic heating plate thermally stimulates the rat skin, it will be affected by the contact surface pressure. The pressure difference will lead to different thermal stimulation effects. Therefore, the pressure control module sets a small area pressure sensor under the heating plate. The Arduino control module uses the pressure value feedback from the module to control the stepper motor to move. When the thermal stimulation starts, the contact surface pressure is controlled to remain within a reasonable range to ensure that the effect of each thermal stimulation is constant. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0022] Figure 1 It is a schematic diagram of the structure of the device of the present invention; Figure 2 It is a schematic diagram of the structure of the thermal stimulation module of the present invention; Figure 3 The three views of the thermal stimulation module of the present invention, wherein (a) is the front view; (b) is the left view; (c) is the top view; Figure 4 It is a principle block diagram of the device of the present invention; Figure 5 The following is a flowchart of a stepper motor control implementation based on an Arduino control module according to an embodiment of the present invention.

[0023] In the figure: 1. Thermal stimulation module; 2. Arduino control module; 3. Stepper motor drive module; 4. Temperature control module; 5. Pressure control module; 6. Power module; 7. Precision stepper motor; 8. Temperature sensor; 9. Round ceramic heating plate; 10. Pressure sensor; 11. Fixed elastic band; 12. Suction cup; 13. Shell; 14. Stepper motor control connection line; 15. Shell side hole; 16. Rectangular mat; 17. Two-hole plug; 18. Type-C interface; 19. Temperature display digital tube. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0025] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0027] In the description of the embodiments of the present invention, it should be noted that if the terms "upper", "lower", "horizontal", "inner", etc. indicate an orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the invention is usually placed when in use, it is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0028] In addition, if the term "horizontal" appears, it does not mean that the component must be absolutely horizontal, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", which does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0029] In the description of the embodiments of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] The present invention is further described in detail below in conjunction with the accompanying drawings: See also Figure 1 and Figure 4 The present application discloses a controllable thermal pain stimulation device for rats, characterized in that: A control unit, used for controlling a thermal pain stimulation experiment according to a preset threshold value; The thermal stimulation module 1 is used to automatically implement the thermal pain stimulation experiment according to the instructions of the control unit and feedback the experimental data; The power module 6 is used to supply power to the control unit.

[0031] The present application adopts a control unit in conjunction with a thermal stimulation module 1 to realize an automated thermal pain stimulation experiment, avoids human intervention in control and operation, and improves the rigor and stability of the experiment.

[0032] In some embodiments, the thermal stimulation module 1 includes: a shell 13, the shell 13 is detachably arranged on a workbench, a fixed elastic band 11 is arranged at the bottom of the shell 13, a stepper motor 7 is arranged on the top of the shell 13, the stepper motor 7 is connected to a control unit, the output end of the stepper motor 7 passes through the shell 13 and is connected to a ceramic heating plate 9, a temperature sensor 8 and a pressure sensor 10 are arranged on the ceramic heating plate 9, and the temperature sensor 8, the ceramic heating plate 9 and the pressure sensor 10 are all electrically connected to the control unit.

[0033] In some embodiments, a side hole 15 is opened on the side wall of the shell 13, and the temperature sensor 8, the ceramic heating plate 9 and the pressure sensor 10 are all electrically connected to the control unit through the side hole 15.

[0034] In some embodiments, the shell 13 is a frame structure that penetrates from front to back, and a padding cloth 16 is provided on the inner bottom thereof. The padding cloth 16 is located below the fixed elastic band 11 .

[0035] In some embodiments, a suction cup 12 is disposed outside the bottom of the shell 13 for fixing the shell 13 on a workbench.

[0036] In some embodiments, the temperature sensor 8 is disposed on the upper surface of the ceramic heating plate 9 , and the pressure sensor 10 is disposed on the lower surface of the ceramic heating plate 9 .

[0037] In some embodiments, the control unit comprises: Arduino control module 2, used for receiving pressure feedback signal and outputting control signal to driving module 3; A driving module 3, used for adjusting the thermal stimulation module 1 according to a control signal; The temperature control module 4 is used to receive temperature feedback and output temperature control to the thermal stimulation module 1; The pressure control module 5 is used to receive the pressure feedback from the thermal stimulation module 1 and transmit the pressure feedback to the Arduino control module 2 .

[0038] In some embodiments, the preset threshold value includes the thermal pain stimulation duration, cycle parameters, preset number of cycles and contact pressure threshold parameters set on the Arduino control module 2.

[0039] In some embodiments, the thermal stimulation module 1 includes: a stepper motor 7, a temperature sensor 8, a ceramic heating plate 9 and a pressure sensor 10, the stepper motor 7 is connected to the ceramic heating plate 9, and the temperature sensor 8 and the pressure sensor 10 are arranged on the ceramic heating plate 9; The driving module 3 is connected to the stepping motor 7 and is used to control the stepping direction and stepping speed of the stepping motor 7; The temperature control module 4 is connected to the temperature sensor 8 and the ceramic heating plate 9, and is used to output temperature control to the ceramic heating plate 9 according to the temperature feedback received from the temperature sensor 8; The pressure control module 5 is connected to the pressure sensor 10 and is used for receiving pressure feedback transmitted by the pressure sensor 10 and transmitting the pressure feedback to the Arduino control module 2 .

[0040] In some embodiments, the driving module 3 is connected to the stepper motor 7 via a stepper motor control connection line 14 .

[0041] In some embodiments, Figure 1 and Figure 2 As shown, a controllable thermal pain stimulation device for rats includes a thermal stimulation module 1, an Arduino control module 2, a drive module 3, a temperature control module 4, a pressure control module 5, and a power supply module 6.

[0042] like Figure 2As shown, the power module 6 is connected to the 220V socket input with a two-hole plug 17 of the power cord, and the other end of the power cord is connected to the power module 6 with a Type-C interface 18, providing a 5V DC power supply to the temperature control module 4 and the pressure control module 5 in the control module to ensure the normal operation of the module. The Arduino control module 2 receives the pressure feedback signal through program design, outputs a control signal that can control the stepper motor drive, and transmits the control signal to the drive module 3, and the drive module 3 controls the direction and speed of the stepper motor. During the operation of the thermal pain stimulation device, the stepper motor 7 and the ceramic heating plate 9 keep synchronous stepping; the ceramic heating plate 9 feeds back the temperature signal to the temperature control module 4, and the temperature control module 4 uses the on-off power supply voltage to provide voltage to the ceramic heating plate 9 to control the temperature fluctuation of the ceramic heating plate 9 within the expected range; the pressure signal of the contact surface between the bottom of the ceramic heating plate 9 and the thermal stimulation part of the rat skin is fed back to the pressure control module 5, and the pressure control module 5 converts the resistance signal into a voltage signal analog output and inputs it into the Arduino control module 2, and strictly controls the travel distance of the stepper motor through program design, thereby controlling the pressure fluctuation of the thermal stimulation contact surface within the expected range.

[0043] like Figure 3 As shown, a stepper motor 7 is fixed on the top of the shell 13, and the bottom is fixed to the experimental table by a suction cup 12; wherein, the stepper motor 7 is fixed on the top of the shell 13, and its two ends are fixed by screws, and its control signal and power supply voltage are transmitted by the stepper motor control connecting line 14, and the top of the telescopic rod is fixedly connected to the ceramic heating plate 9 by hot melt adhesive, and a temperature sensor 8 is connected above the ceramic heating plate 9 to feed back the temperature of the ceramic heating plate 9 in real time, and its signal line is transmitted from the side hole 15 of the shell, and a pressure sensor 10 is contacted below to feed back the contact surface pressure between the ceramic heating plate 9 and the thermal stimulation part of the rat in real time, and its signal line is transmitted from the side hole 15 of the shell; below the ceramic heating plate 10, a rectangular pad 16 is placed at the bottom of the shell 13, and the stimulation part of the rat can be aligned and placed here, and further fixed by a fixed elastic band 11 to ensure the experimental effect of thermal pain stimulation corresponding to the target skin part of the rat.

[0044] In some embodiments, Figure 5As shown, the flow chart of the stepper motor control based on the Arduino control module 2 is as follows: before starting the stimulation, define the control signal pin, set the thermal pain stimulation duration, cycle parameters, number of stimulations, and appropriate contact pressure threshold parameters, and enter the loop cycle; first, the program obtains the system time, and uses the start time of the last cycle to first determine whether the conditions for starting a cycle are met, that is, whether the distance from the last thermal pain stimulation is enough for one cycle. If so, the stepper motor is controlled to start forward stepping, otherwise continue to judge. When the stepper motor steps forward, the degree determines whether the pressure feedback signal between the heating plate and the rat skin stimulation site reaches the threshold. If the threshold is reached, it means that the ceramic heating plate and the rat skin stimulation site are in good contact, stop stepping and start thermal pain stimulation, and the delay time is the expected stimulation duration. If it is not reached, continue to cycle the above process; after the stimulation is over, the stepper motor steps 30mm in the opposite direction, and records the start time of this stimulation cycle, and updates the number of cycles. The program determines whether the expected number of cycles is reached. If so, the program ends, otherwise the program enters waiting for the end of this cycle and determines whether to enter the next stimulation cycle. The whole process strictly controls one cycle to be the preset period parameter, and strictly controls the preset number of cycles.

[0045] The implementation is as follows: In actual use, before the thermal pain stimulation of the rat begins, the target skin part of the thermal pain stimulation of the rat is fixed to the rectangular pad 16 at the bottom of the housing 13, and the stimulation position is fixed with a fixed elastic band 11. The power module 6 is connected, the temperature control module 4 and the pressure control module 5 start working, the thermal pain stimulation temperature and temperature fluctuation range parameters are preset in the temperature control module 4, the Arduino control module 2 is connected to the computer, the control signal pin, stimulation duration, interval period, number of stimulation cycles, contact pressure threshold and other parameters are preset, and after running correctly, the program is copied to the Arduino, and the thermal pain stimulation process of the rat can be started.

[0046] During the operation of the thermal pain stimulation device, the ceramic heating plate 9 cyclically stimulates the rat with a fixed duration and period, and the stepper motor 7 and the ceramic heating plate 9 perform reciprocating stepping motions to stimulate the target skin part of the rat with a constant contact pressure, a constant temperature, and a fixed duration. The parameters of the device are immutable during operation, and the entire process is automatic and does not require human intervention until the stimulation process ends. When the device stops thermal pain stimulation, the operation ends and the power supply is disconnected.

[0047] The present application is a thermal pain stimulation device for rats in an open environment. After the parameters are pre-set, thermal pain stimulation can be automatically performed. The operation is simple and does not require human intervention. The preparation process, working process, and ending process of the device are relatively convenient and simple. The overall structure of the device is clear and has a flexible change space. The device can avoid spatial obstruction of the device to other collection equipment and fixed equipment while satisfying the user's flexible setting of temperature, duration, cycle, number of times, etc., and achieves effective thermal pain stimulation on the rat skin. It is suitable for thermal pain stimulation in many experimental operation situations.

[0048] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A controllable thermal pain stimulation device for rats, characterized in that: include: A control unit, used for controlling a thermal pain stimulation experiment according to a preset threshold value; The thermal stimulation module (1) is used to automatically implement the thermal pain stimulation experiment according to the instructions of the control unit and to feed back the experimental data; The power module (6) is used to supply power to the control unit.

2. A controllable thermal pain stimulation device for rats according to claim 1, characterized in that: The thermal stimulation module (1) comprises: a housing (13), the housing (13) being detachably arranged on a workbench, a fixed elastic band (11) being arranged in the bottom of the housing (13), a stepping motor (7) being arranged on the top of the housing (13), the stepping motor (7) being connected to a control unit, an output end of the stepping motor (7) passing through the housing (13) and being connected to a ceramic heating plate (9), a temperature sensor (8) and a pressure sensor (10) being arranged on the ceramic heating plate (9), and the temperature sensor (8), the ceramic heating plate (9) and the pressure sensor (10) being all electrically connected to the control unit.

3. A controllable thermal pain stimulation device for rats according to claim 2, characterized in that: A side hole (15) is provided on the side wall of the housing (13), and the temperature sensor (8), the ceramic heating plate (9) and the pressure sensor (10) are all electrically connected to the control unit via the side hole (15).

4. A controllable thermal pain stimulation device for rats according to claim 2, characterized in that: The shell (13) is a frame structure that penetrates from front to back, and a padding cloth (16) is provided on the inner bottom thereof, and the padding cloth (16) is located below the fixed elastic band (11).

5. A controllable thermal pain stimulation device for rats according to claim 2, characterized in that: A suction cup (12) is arranged outside the bottom of the shell (13) and is used to fix the shell (13) on a workbench.

6. A controllable thermal pain stimulation device for rats according to claim 2, characterized in that: The temperature sensor (8) is arranged on the upper surface of the ceramic heating plate (9), and the pressure sensor (10) is arranged on the lower surface of the ceramic heating plate (9).

7. A controllable thermal pain stimulation device for rats according to claim 1, characterized in that: The control unit comprises: An Arduino control module (2) is used to receive a pressure feedback signal and output a control signal to a drive module (3); A driving module (3) for adjusting the thermal stimulation module (1) according to a control signal; A temperature control module (4), used for receiving temperature feedback and outputting temperature control to the thermal stimulation module (1); The pressure control module (5) is used to receive pressure feedback from the thermal stimulation module (1) and transmit the pressure feedback to the Arduino control module (2).

8. A controllable thermal pain stimulation device for rats according to claim 7, characterized in that: The preset threshold value includes a thermal pain stimulation duration, a cycle parameter, a preset number of cycles and a contact pressure threshold parameter set on the Arduino control module (2).

9. A controllable thermal pain stimulation device for rats according to claim 1, characterized in that: The thermal stimulation module (1) comprises: a stepping motor (7), a temperature sensor (8), a ceramic heating plate (9) and a pressure sensor (10); the stepping motor (7) is connected to the ceramic heating plate (9), and the temperature sensor (8) and the pressure sensor (10) are arranged on the ceramic heating plate (9); The driving module (3) is connected to the stepping motor (7) and is used to control the stepping direction and stepping speed of the stepping motor (7); The temperature control module (4) is connected to the temperature sensor (8) and the ceramic heating plate (9) and is used to output temperature control to the ceramic heating plate (9) according to the temperature feedback received from the temperature sensor (8); The pressure control module (5) is connected to the pressure sensor (10) and is used for receiving pressure feedback transmitted by the pressure sensor (10) and transmitting the pressure feedback to the Arduino control module (2).

10. A controllable thermal pain stimulation device for rats according to claim 9, characterized in that: The driving module (3) is connected to the stepping motor (7) via a stepping motor control connecting line (14).

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