Plant extract composition for synergistically repairing skin barrier

Abstract

The invention provides a Chinese herbal medicine composition. The composition comprises burdock, gentian, radix stephaniae tetrandrae and centella asiatica as raw materials or extracts of burdock, gentian, radix stephaniae tetrandrae and centella asiatica. The invention also relates to a preparation method of the Chinese herbal medicine composition, an application of the Chinese herbal medicine composition in repairing a skin barrier and a medicine or a cosmetic containing the Chinese herbal medicine composition.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and relates to a Chinese herbal medicine composition, wherein the composition is made of or contains extracts of burdock fruit, gentian, stephania tetrandra and centella asiatica. The present invention also relates to a preparation method and use of the Chinese herbal medicine composition. The present invention further relates to a medicine or cosmetic containing the Chinese herbal medicine composition. Background Art

[0002] As the largest organ of the human body, the skin bears the important task of resisting harmful damage from the external environment and maintaining the health of the body. When the homeostasis of the skin barrier is damaged, the skin will appear in a sensitive metastable state, and in severe cases it may even lead to various skin diseases such as atopic dermatitis and psoriasis. As the number of people with sensitive skin continues to increase worldwide, people are paying more and more attention to the repair of sensitive skin. Therefore, repairing the skin barrier is currently a key development direction in the field of skin science and cosmetic application research.

[0003] The skin is mainly composed of four major barriers: physical barrier, chemical barrier, microbial barrier and immune barrier. Among them, the integrity of the physical barrier is jointly determined by the stratum corneum and the tight junction barrier, which is the basis for the other three barriers to play a normal physiological role. Therefore, repairing the physical barrier in the skin is crucial to improve sensitive skin and maintain skin health.

[0004] Keratinocytes are the main component of the skin barrier. Filaggrin encoded by FLG is an important marker protein at the end of keratinocyte differentiation, and its decomposition product, natural moisturizing factor (NMF), is a water-soluble hygroscopic component of the skin. Therefore, filaggrin plays an important role in maintaining normal pH, hydration capacity and mechanical integrity of the stratum corneum. Tight junction proteins are a type of structural protein that connects keratinocytes and prevents solute penetration in the epidermis. They have material and ion size selectivity, so they are also called tight junction barriers. The tight junction barrier mainly includes Occludins, Claudins and ZO molecular families, among which Claudin-1 and Zonula occludens-1 (ZO-1) are the main transmembrane and cytoplasmic proteins in tight junction proteins, respectively. ZO-1 can connect transmembrane proteins to the cytoskeleton to transmit various signals to cells. When the skin barrier is damaged, the stratum corneum and tight junctions are destroyed, resulting in reduced levels of Filaggrin, Claudin-1 and ZO-1, impaired skin mechanical integrity, hydration function, and epidermal permeability, making it easier for external stimuli to penetrate the skin, thereby triggering an inflammatory response, further promoting barrier damage, and ultimately leading to skin sensitivity. Therefore, for sensitive skin damaged barrier repair, it is crucial to improve the physical barrier (especially the tight junction barrier).

[0005] At present, there are various technologies for skin barrier repair. For example, CN202211017250 discloses a composition and its application with synergistic effect of repairing skin barrier, the composition includes inca inchi seed oil and ceramide, and the composition involved achieves synergistic improvement of gene expression level and transcutaneous resistance of skin barrier-related proteins by supplementing structural lipids, showing the effect of repairing skin barrier; CN202311161297 discloses a grease composition that can promote skin barrier function, the composition involved includes sea buckthorn seed oil and sunflower seed oil as active ingredients, which can increase the aggregation force of corneocytes and improve skin barrier function by enhancing one of the main proteins of tight junctions of keratinocytes-demosome protein. However, the above inventions all achieve the effect of repairing barrier function by compounding fat-soluble components, and there are problems such as poor stability of fat-soluble component formulas and inability to meet the application of some dosage forms.

[0006] Based on this, it is very important to develop a stable water-soluble plant extract composition that can repair the skin barrier by improving the damaged skin physical barrier. Summary of the invention

[0007] In view of the deficiencies in the prior art, the present invention aims to provide a Chinese herbal medicine composition having the function of repairing the skin barrier and a preparation method thereof. The Chinese herbal medicine composition of the present invention has good stability and water solubility, and each component has the function of synergistically repairing the skin barrier function.

[0008] The objective of the present invention is achieved through the following technical solutions:

[0009] The first aspect of the present invention relates to a Chinese herbal medicine composition, wherein the composition is made from the following raw materials or contains extracts thereof: arctium lappa, gentiana scabra, stephania tetrandra and centella asiatica.

[0010] The second aspect of the present invention relates to a method for preparing the Chinese herbal medicine composition according to the first aspect of the present invention, comprising the following steps:

[0011] (1) weighing and crushing a burdock fruit raw material, and extracting with a solvent to obtain a burdock fruit extract;

[0012] (2) weighing and crushing gentian, fangji and centella asiatica, extracting them separately or together with a solvent and then combining them to obtain a combined extract of gentian, fangji and centella asiatica;

[0013] (3) Mixing the extracts obtained in steps (1) and (2) to obtain the composition.

[0014] The third aspect of the present invention relates to use of the Chinese herbal composition according to the first aspect of the present invention or the Chinese herbal composition prepared according to the method of the second aspect of the present invention in the preparation of medicines or cosmetics for repairing the skin barrier.

[0015] The fourth aspect of the present invention relates to the use of a combined extract of Gentiana scabra, Stephania tetrandra and Centella asiatica in the preparation of a medicine or cosmetic for repairing the skin barrier.

[0016] The fifth aspect of the present invention relates to a medicine or cosmetic, wherein the medicine or cosmetic comprises the Chinese herbal composition described in the first aspect of the present invention or the Chinese herbal composition prepared according to the method of the second aspect of the present invention or the combined extract of Gentiana, Stephania tetrandra and Centella asiatica as an active ingredient, and one or more pharmaceutically or cosmetically acceptable carriers, diluents or excipients.

[0017] The present invention has the following beneficial effects:

[0018] 1. The Chinese herbal medicine composition of the present invention can enhance the expression of filaggrin gene (FLG) and tight junction protein gene (ZO-1, CLDN1) in the barrier damaged cell model, thereby repairing the physical barrier damage of the skin barrier caused by ultraviolet rays and repairing the skin barrier function;

[0019] 2. When the weight of arctium fruit or its extract in the Chinese herbal medicine composition of the present invention is combined with gentiana, stephania tetrandra and centella asiatica or their extracts in a specific weight ratio, a synergistic barrier repair effect can be achieved, effectively improving the problems of obvious ineffectiveness of the barrier repair effect of using a single component (such as using arctium fruit or its extract alone or using gentiana, stephania tetrandra and centella asiatica or their extracts alone) and high addition concentration, and effectively reducing the cost.

[0020] 3. The applicant also unexpectedly found that the combined extract of gentiana, stephania tetrandra and centella asiatica can effectively repair the damage of filaggrin gene (FLG) expression caused by ultraviolet rays and play a role in skin barrier repair.

[0021] 4. The Chinese herbal medicine composition involved in the present invention has good water solubility and stability, strong formula adaptability, and can be applied to various cosmetic dosage forms. Brief Description of the Drawings

[0022] Figure 1 Shows the effect of arctium fruit extract (component a) on the viability of HaCaT cells.

[0023] Figure 2 Shows the effect of the combined extract of gentiana, stephania tetrandra and centella asiatica (component b) on the viability of HaCaT cells.

[0024] Figure 3 Shows the effect of different compositions on the expression level of FLG gene (compared with the blank group, #: p < 0.05; compared with Comparative Example 2, *: p < 0.05, **: 0.01 < p < 0.05).

[0025] Figure 4 Shows the effect of different compositions on the expression level of ZO-1 gene (compared with the blank group, : p < 0.001; compared with Comparative Example 1, *: p < 0.05, **: 0.01 < p < 0.05).

[0026] Figure 5 Shows the effect of different compositions on the expression level of CLDN1 gene (compared with the blank group, ##: 0.01 < p < 0.05; compared with Comparative Example 1, **: 0.01 < p < 0.05, ***: p < 0.001). Detailed Description of the Invention

[0027] Definition

[0028] The terms "Chinese herbal medicine composition" and "composition" used herein have similar meanings and can be used interchangeably without special instructions.

[0029] In this document, the terms "weight %" and "weight percentage" are used interchangeably. If not otherwise specified, percentage (%) refers to weight percentage. The terms "parts by weight" or "parts by weight" refer to the weight ratio between components. For example, 1 part of component A and 2 parts of component B means that the ratio of component A to component B is 1:2.

[0030] The term "room temperature" used herein refers to 25°C ± 1°C. Meanwhile, if no experimental temperature is specifically specified, it is room temperature.

[0031] As used herein, the terms "s", "min" and "h" stand for "seconds", "minutes" and "hours", respectively.

[0032] The term "about" as used herein refers to ±10%, more preferably ±5%, and most preferably ±2% of the value modified by the term, so that a person skilled in the art can clearly determine the scope of the term "about" based on the modified value.

[0033] As used herein, the term "active ingredient" refers to a compound that provides a benefit or improvement to the skin when applied to the skin. It should be understood that active ingredients are not only for application to the skin, but also for application to hair, nails and other mammalian keratinous tissues.

[0034] As used herein, the term "cosmetically acceptable excipients" refers to excipients that are not necessary active ingredients in cosmetics and are suitable for use in humans and / or animals without excessive adverse side effects (such as toxicity, irritation, and allergic reactions), and have a reasonable benefit / risk ratio. Typically, cosmetically acceptable excipients may include moisturizers, emulsifiers, thickeners, chelating agents, emollients, and the like.

[0035] As used herein, the term "pharmaceutically acceptable excipient" refers to an excipient that is not a necessary active ingredient in a drug itself and is suitable for administration to humans and / or animals without excessive adverse side effects (such as toxicity, irritation, and allergic reactions), and has a reasonable benefit / risk ratio. Typically, pharmaceutically acceptable excipients may include fillers, stabilizers, diluents, adhesives, surfactants, solvents, etc.

[0036] The term "crushing" used herein means crushing raw materials together or separately.

[0037] The term "extraction" as used herein means extracting the raw materials together or separately.

[0038] Burdock seeds are the dried mature fruits of Arctium lappa L., a plant of the Asteraceae family.

[0039] Gentiana is the dried root or rhizome of Gentiana scabra Bunge, a plant of the Gentianaceae family.

[0040] Stephania tetrandra is the dried root of Stephania tetrandra S.Moore, a plant of the Menispermaceae family.

[0041] Centella asiatica is the dried whole herb of Centella asiatica (L.) Urban of the Umbelliferae family.

[0042] Composition

[0043] The first aspect of the present invention relates to a Chinese herbal medicine composition, wherein the composition is made from the following raw materials or contains extracts thereof: arctium lappa, gentiana scabra, stephania tetrandra and centella asiatica.

[0044] In some embodiments, the composition comprises a burdock fruit extract and a combined extract of gentian, stephens and centella asiatica. In a specific embodiment, the burdock fruit extract is referred to as component a and the combined extract of gentian, stephens and centella asiatica is referred to as component b.

[0045] In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephen cao and centella asiatica or their extracts is in the range of 6:1 to 1:12. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephen cao and centella asiatica or their extracts is in the range of 4:1 to 1:10.

[0046] In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania and centella asiatica or their extracts is 6:1. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania and centella asiatica or their extracts is 5:1. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania and centella asiatica or their extracts is 4:1. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania and centella asiatica or their extracts is 3:1. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania and centella asiatica or their extracts is 2:1. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania and centella asiatica or their extracts is 1:1. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephen cao and centella asiatica or their extracts is 1:2. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephen cao and centella asiatica or their extracts is 1:3. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephen cao and centella asiatica or their extracts is 1:4. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephen cao and centella asiatica or their extracts is 1:5. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephen cao and centella asiatica or their extracts is 1:6. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephen cao and centella asiatica or their extracts is 1:7. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania tetrandra and centella asiatica or their extracts is 1:8. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania tetrandra and centella asiatica or their extracts is 1:9. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania tetrandra and centella asiatica or their extracts is 1:10. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania tetrandra and centella asiatica or their extracts is 1:11. In some embodiments, the weight ratio of burdock seeds or its extract to the weight of gentian, stephania tetrandra and centella asiatica or their extracts is 1:12.

[0047] In some embodiments, the burdock seed raw material in the Chinese herbal composition of the present invention is 1, 2, 3, 4, 5, 6 parts by weight and any range between the above parts by weight; the combination of gentian, stephania tetrandra and centella asiatica raw materials is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 parts by weight and any range between the above parts by weight. For example, the Chinese herbal composition of the present invention is made of the following raw materials by weight: 1-6 parts of burdock seeds, 1-12 parts of the combination of gentian, stephania tetrandra and centella asiatica; or 1-5 parts of burdock seeds, 1-11 parts of the combination of gentian, stephania tetrandra and centella asiatica; or 1-4 parts of burdock seeds, 1-10 parts of the combination of gentian, stephania tetrandra and centella asiatica.

[0048] In some embodiments, the burdock extract in the Chinese herbal composition of the present invention is 1, 2, 3, 4, 5, 6 parts by weight and any range between the above parts by weight; the combined extract of gentian, stephania tetrandra and centella asiatica is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 parts by weight and any range between the above parts by weight. For example, the Chinese herbal composition of the present invention comprises the following extracts by weight: 1-6 parts of burdock extract, 1-12 parts of the combined extract of gentian, stephania tetrandra and centella asiatica; or 1-5 parts of burdock extract, 1-11 parts of the combined extract of gentian, stephania tetrandra and centella asiatica; or 1-4 parts of burdock extract, 1-10 parts of the combined extract of gentian, stephania tetrandra and centella asiatica.

[0049] In some embodiments, the combination of gentian, Stephania tetrandra and Centella asiatica comprises gentian, Stephania tetrandra and Centella asiatica in the following weight ratio: gentian 3-7: Stephania tetrandra 1-4: Centella asiatica 1-4. In a preferred embodiment, the combination of gentian, Stephania tetrandra and Centella asiatica comprises gentian, Stephania tetrandra and Centella asiatica in the following weight ratio: gentian 4-6.5: Stephania tetrandra 1.5-3: Centella asiatica 1.5-3. In some embodiments, the combination of gentian, Stephania tetrandra and Centella asiatica comprises gentian, Stephania tetrandra and Centella asiatica in the following weight ratio: gentian 6: Stephania tetrandra 2.5: Centella asiatica 1.5. In some embodiments, the combination of gentian, Stephania tetrandra and Centella asiatica comprises gentian, Stephania tetrandra and Centella asiatica in the following weight ratio: gentian 5: Stephania tetrandra 2.5: Centella asiatica 2.5. In some embodiments, the combination of Gentiana, Stephania Tetrandra and Centella asiatica comprises Gentiana, Stephania Tetrandra and Centella asiatica in the following weight ratio: Gentiana 4.5: Stephania Tetrandra 3: Centella asiatica 1.5.

[0050] In some embodiments, in the combination of Gentiana, Stephania Tetrandra and Centella asiatica, the amount of Gentiana by weight is 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7 parts; the amount of Stephania Tetrandra by weight is 1, 1.5, 2, 2.5, 3, 3.5, 4 parts; and the amount of Centella asiatica by weight is 1, 1.5, 2, 2.5, 3, 3.5, 4 parts.

[0051] In some embodiments, the combined extract of Gentiana, Stephania Tetrandra and Centella asiatica comprises 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, and 7 parts by weight of Gentiana extract; 1, 1.5, 2, 2.5, 3, 3.5, and 4 parts by weight of Stephania Tetrandra extract; and 1, 1.5, 2, 2.5, 3, 3.5, and 4 parts by weight of Centella asiatica extract.

[0052] In some embodiments, the combined extract of gentian, tetrandrin and Centella asiatica contains gentiopicroside, tetrandrin A and total glycosides of Centella asiatica. In some embodiments, the weight ratio of gentiopicroside, tetrandrin A and total glycosides of Centella asiatica is about: gentiopicroside 3-7: tetrandrin A 1-4: total glycosides of Centella asiatica 1-4. In some embodiments, the weight ratio of gentiopicroside, tetrandrin A and total glycosides of Centella asiatica is about: gentiopicroside 4-6: tetrandrin A 1-3: total glycosides of Centella asiatica 1-3. In some embodiments, the weight ratio of gentiopicroside, tetrandrin A and total glycosides of Centella asiatica is about: gentiopicroside 4-6.5: tetrandrin A 1.5-3: total glycosides of Centella asiatica 1.5-3.

[0053] Preparation method

[0054] The second aspect of the present invention relates to a method for preparing the Chinese herbal medicine composition according to the first aspect of the present invention, which comprises weighing and crushing the raw materials of burdock seeds, gentian, stephania tetrandra and Centella asiatica, extracting with a solvent, and obtaining the extract of the present invention.

[0055] In some embodiments, the methods of the present invention include the following steps:

[0056] (1) weighing and crushing a burdock fruit raw material, and extracting with a solvent to obtain a burdock fruit extract;

[0057] (2) weighing and crushing gentian, Stephania tetrandra and Centella asiatica, and extracting with a solvent to obtain a combined extract of gentian, Stephania tetrandra and Centella asiatica;

[0058] (3) Mixing the extracts obtained in steps (1) and (2) to obtain the composition.

[0059] In some embodiments, the methods of the present invention include the following steps:

[0060] (1) weighing and crushing the burdock fruit raw material, and extracting with a solvent to obtain the extract of the present invention;

[0061] (2) weighing gentian, Stephania tetrandra and Centella asiatica and crushing them separately, extracting them with solvents respectively and then combining them to obtain a combined extract of gentian, Stephania tetrandra and Centella asiatica;

[0062] (3) Mixing the extracts obtained in steps (1) and (2) to obtain the composition.

[0063] In some embodiments, the weight ratio of burdock seeds or its extract to the weight ratio of gentian, stephen cao and centella asiatica or its extract is in the range of 6: 1 to 1: 12. In some embodiments, the weight ratio of burdock seeds or its extract to the weight ratio of gentian, stephen cao and centella asiatica or its extract is in the range of 4: 1 to 1: 10.

[0064] In some embodiments, the combination of gentian, Stephania tetrandra and Centella asiatica comprises gentian, Stephania tetrandra and Centella asiatica in the following weight ratio: gentian 3-7: Stephania tetrandra 1-4: Centella asiatica 1-4. In a preferred embodiment, the combination of gentian, Stephania tetrandra and Centella asiatica comprises gentian, Stephania tetrandra and Centella asiatica in the following weight ratio: gentian 4-6.5: Stephania tetrandra 1.5-3: Centella asiatica 1.5-3.

[0065] In some embodiments, the solvent in step (1) is an aqueous solution of 50-90% by volume of alcohol. In some embodiments, the solvent in step (1) is an aqueous solution of 60-80% by volume of alcohol. In some embodiments, the temperature of extraction in step (1) is about 60-90°C. In some embodiments, the temperature of extraction in step (1) is 70-80°C. In some embodiments, the number of extractions in step (1) is 1-3 times. In some embodiments, the number of extractions in step (1) is 2 times. In some embodiments, the duration of each extraction in step (1) is 30-90min. In some embodiments, the duration of each extraction in step (1) is preferably 60-80min. In some embodiments, the weight ratio of the burdock seed raw material to the solvent in step (1) is 1:6-1:15. In some embodiments, the weight ratio of the burdock seed raw material to the solvent in step (1) is 1:8-1:12.

[0066] In some embodiments, step (1) further comprises a step of sieving after pulverizing. In some embodiments, the sieve is a 20-80 mesh sieve. In some embodiments, the sieve is a 20-40 mesh sieve.

[0067] In some embodiments, step (1) further comprises the step of refining the burdock fruit extract after extraction. In some embodiments, the step of refining the burdock fruit extract comprises concentrating the burdock fruit extract to remove the solvent, diluting it with water and filtering it, adsorbing it on a non-polar macroporous resin, and washing and eluting it with a solvent, respectively, to obtain a refined burdock fruit extract.

[0068] In some embodiments, in the step of refining burdock extract, water is added to dilute to 3-10 times the equivalent volume of burdock extract. In some embodiments, in the step of refining burdock extract, water is added to dilute to 5-7 times the equivalent volume of burdock extract. In some embodiments, in the step of refining burdock extract, rinsing is carried out with an aqueous solution of 0-20% by volume of alcohol. In some embodiments, in the step of refining burdock extract, rinsing is carried out with an aqueous solution of 0-10% by volume of alcohol. In some embodiments, in the step of refining burdock extract, elution is carried out with an aqueous solution of 50-90% by volume of alcohol. In some embodiments, in the step of refining burdock extract, elution is carried out with an aqueous solution of 70-80% by volume of alcohol. In some embodiments, the non-polar resin macroporous resin is selected from D101, AB-8, HPD-100 and XDA-8. In some embodiments, the non-polar macroporous resin is AB-8.

[0069] In some embodiments, the aqueous solution of alcohol is C 2-6 In some embodiments, the C 2-6 The alcohol is selected from ethanol, propanol, isopropanol, propylene glycol, n-butanol or a combination thereof. 2-6 The alcohol is ethanol.

[0070] In some embodiments, in step (2), the extraction is performed three times, and the extracts after the three extractions are mixed to obtain a combined extract of Gentiana, Stephania tetrandra and Centella asiatica. In some embodiments, the extraction includes water extraction, a first alcohol aqueous solution extraction and a second alcohol aqueous solution extraction.

[0071] In some embodiments, the first alcohol aqueous solution extraction uses an alcohol aqueous solution of 20-70% by volume. In some embodiments, the second alcohol aqueous solution extraction uses an alcohol aqueous solution of 50-70% by volume.

[0072] In some embodiments, the water extraction temperature is 60-80°C. In some embodiments, the alcohol aqueous solution extraction temperature is 40-85°C. In some embodiments, the alcohol aqueous solution is C 2-6 In some embodiments, the C 2-6 The alcohol is selected from ethanol, propanol, propylene glycol, n-butanol or a combination thereof. 2-6 The alcohol is selected from propylene glycol and ethanol.

[0073] In some embodiments, the method for preparing the plant extract further comprises filtering the obtained combined extract of Gentiana, Stephania tetrandra and Centella asiatica for purification, and obtaining a concentrated solution. In some embodiments, the filtration is membrane filtration. In some embodiments, first filter with a 5000-10000 Dalton ultrafiltration membrane, collect the filtrate, then filter with a 100-1000 Dalton nanofiltration membrane, recover the solvent, and obtain a concentrated solution. In some embodiments, first filter with a 5000 Dalton ultrafiltration membrane, collect the filtrate, then filter with a 1000 Dalton nanofiltration membrane, recover the solvent, and obtain a concentrated solution.

[0074] In some embodiments, the method for preparing a plant extract further comprises drying the resulting concentrate, and optionally, before drying, adding water to the concentrate. In some embodiments, the drying is selected from freeze drying, vacuum drying, spray drying, or a combination thereof.

[0075] In some embodiments, in the method for preparing a plant extract, an external circulation extraction method is used, and an online spectrophotometer is used for monitoring. The end point is determined based on the absorbance, and the residue is removed at that time to obtain an extract.

[0076] use

[0077] The third aspect of the present invention relates to use of the Chinese herbal composition according to the first aspect of the present invention or the Chinese herbal composition prepared according to the method of the second aspect of the present invention in the preparation of medicines or cosmetics for repairing the skin barrier.

[0078] The fourth aspect of the present invention relates to the use of a combined extract of gentian, stephania and centella in the preparation of a medicament or cosmetic for repairing the skin barrier. In some embodiments, the combined extract is made of or contains the following weight ratios of gentian, stephania and centella: gentian 3-7: stephania 1-4: centella 1-4. In some embodiments, the combined extract is made of or contains the following weight ratios of gentian, stephania and centella: gentian 4-6.5: stephania 1.5-3: centella 1.5-3. In some embodiments, the combined extract is made of or contains the following weight ratios of gentian, stephania and centella: gentian 6: stephania 2.5: centella 1.5. In some embodiments, the combined extract is made of or comprises extracts of Gentiana, Stephania Tetrandra and Centella Asiatica in the following weight ratio: Gentiana 5: Stephania Tetrandra 2.5: Centella Asiatica 2.5. In some embodiments, the combined extract is made of or comprises extracts of Gentiana, Stephania Tetrandra and Centella Asiatica in the following weight ratio: Gentiana 4.5: Stephania Tetrandra 3: Centella Asiatica 1.5.

[0079] Medicines or cosmetics

[0080] The fifth aspect of the present invention relates to a medicine or cosmetic, wherein the medicine or cosmetic comprises the Chinese herbal composition described in the first aspect of the present invention or the Chinese herbal composition prepared according to the method of the second aspect of the present invention or the combined extract of Gentiana, Stephania tetrandra and Centella asiatica as an active ingredient, and one or more pharmaceutically or cosmetically acceptable carriers, diluents or excipients.

[0081] In some embodiments, the herbal composition accounts for 0.1-10% by weight of the drug or cosmetic. In some embodiments, the herbal composition accounts for 0.1-2% by weight of the drug or cosmetic. In some embodiments, the herbal composition accounts for 0.1-0.5% by weight of the drug or cosmetic.

[0082] In some embodiments, the composition comprises about 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% or 10% by weight of the pharmaceutical or cosmetic product, or any range therebetween.

[0083] In some embodiments, the medicine or cosmetic is an emulsion, lotion, cream, ointment, gel, lotion or essence. In some embodiments, the medicine or cosmetic of the present invention may also contain one or more other ingredients, such as plant extracts, nutritional additives, surfactants, flavors and fragrances, pigments, preservatives, antioxidants, moisturizers, ultraviolet absorbers, astringents, penetration enhancers, pH regulators, etc. Those skilled in the art can make selections based on their common sense and specific needs.

[0084] In some embodiments, the one or more cosmetically acceptable carriers, diluents, or excipients include a thickener, a humectant, an emollient, an emulsifier, a pH adjuster, a chelating agent, or a combination of one or more thereof.

[0085] Example

[0086] The present invention can be implemented through the following embodiments, but the present invention is not limited thereto.

[0087] The instruments and equipment used in the embodiments of the present invention are all conventional instruments and equipment in the field and can be replaced by instruments and equipment that meet the corresponding standards.

[0088] Unless otherwise specified, the reagents used in the embodiments of the present invention are all commercially available analytically pure reagents.

[0089] The raw materials and instruments and equipment mentioned in the present invention are all commonly used raw materials and instruments and equipment in the field, which are only examples and are not intended to limit the scope of protection of the present invention. Those skilled in the art can select equivalent raw materials and related instruments and equipment based on the disclosure of the present invention.

[0090] Component Preparation Example 1: Preparation of Burdock Fruit Extract (Component a)

[0091] Take 500g of burdock fruit medicinal material, crush it into 20 mesh coarse powder, use 90% ethanol as extraction solvent, heat and extract the burdock fruit medicinal material, the extraction temperature is about 70°C, the extraction time is about 90min, the extraction is repeated twice, the weight ratio of burdock fruit medicinal material to solvent is 1:6, collect the extract, filter, concentrate, and dry to obtain the burdock fruit extract.

[0092] Component Preparation Example 2: Preparation of Burdock Fruit Extract (Component a)

[0093] Take 500g of burdock fruit medicinal material, crush it into 20 mesh coarse powder, use 50% ethanol as extraction solvent, reflux extract the burdock fruit medicinal material, the extraction temperature is about 90°C, the extraction time is about 90min, the extraction is repeated twice, the weight ratio of burdock fruit medicinal material to solvent is 1:15, collect the extract, filter, concentrate, and dry to obtain the burdock fruit extract.

[0094] Component Preparation Example 3: Preparation of Burdock Fruit Extract (Component a)

[0095] Take 500g of burdock fruit medicinal material, crush it into 40 mesh coarse powder, use 60% ethanol as extraction solvent, reflux extract the burdock fruit medicinal material, the extraction temperature is about 80°C, the extraction time is about 80min, the extraction is repeated twice, the weight ratio of burdock fruit medicinal material to solvent is 1:12, collect the extract, filter, concentrate, and dry to obtain the burdock fruit extract.

[0096] Component Preparation Example 4: Preparation of Burdock Fruit Extract (Component a)

[0097] Take 500g of burdock fruit medicinal material, crush it into 20 mesh coarse powder, use 80% ethanol as extraction solvent, reflux extract the burdock fruit medicinal material, the extraction temperature is about 80°C, the extraction time is about 60min, the extraction is repeated twice, the weight ratio of burdock fruit medicinal material to solvent is 1:10, collect the extract, filter, concentrate, and dry to obtain the burdock fruit extract.

[0098] Component Preparation Example 5: Preparation of Burdock Fruit Extract (Component a)

[0099] Take 500g of burdock fruit medicinal material, crush it into 20 mesh coarse powder, use 90% ethanol as extraction solvent, reflux extract the burdock fruit medicinal material, the extraction temperature is about 90°C, the extraction time is about 30min, the extraction is repeated twice, the weight ratio of burdock fruit medicinal material to solvent is 1:10, collect the extract, filter, concentrate, and dry to obtain the burdock fruit extract.

[0100] Component Preparation Example 6: Preparation of Burdock Fruit Extract (Component a)

[0101] Take 500g of burdock fruit medicinal material, crush it into 20 mesh coarse powder, use 70% ethanol as extraction solvent, reflux extract the burdock fruit medicinal material, the extraction temperature is about 80°C, the extraction time is about 60min, the extraction is repeated twice, the weight ratio of burdock fruit medicinal material to solvent is 1:8, collect the extract, filter and concentrate, and dry to obtain the burdock fruit extract.

[0102] Component Preparation Example 7: Purification of Burdock Fruit Extract (Component a)

[0103] After extracting the burdock fruit medicinal material according to component preparation example 6, the extracts are combined and concentrated until there is no alcohol taste, diluted with water to 3 times the equivalent volume of the concentrate, filtered, loaded with AB-8 non-polar macroporous resin, rinsed with water, and then eluted with about 50% ethanol aqueous solution, the eluate is collected, concentrated and filtered, and dried to obtain a refined burdock fruit extract.

[0104] Component Preparation Example 8: Purification of Burdock Fruit Extract (Component a)

[0105] After extracting the burdock fruit medicinal material according to component preparation example 6, the extracts are combined and concentrated until there is no alcohol taste, diluted with water to 10 times the equivalent volume of the concentrate, filtered, loaded with AB-8 non-polar macroporous resin, rinsed with 20% ethanol, and then eluted with about 90% ethanol aqueous solution, the eluate is collected, concentrated and filtered, and dried to obtain a refined burdock fruit extract.

[0106] Component Preparation Example 9: Purification of Burdock Fruit Extract (Component a)

[0107] After extracting the burdock fruit medicinal material according to component preparation example 6, the extracts are combined and concentrated until there is no alcohol taste, diluted with water to 5 times the equivalent volume of the concentrate, filtered, loaded with AB-8 non-polar macroporous resin, rinsed with 10% ethanol, and then eluted with about 80% ethanol aqueous solution, the eluate is collected, concentrated and filtered, and dried to obtain a refined burdock fruit extract.

[0108] Component Preparation Example 10: Purification of Burdock Fruit Extract (Component a)

[0109] After extracting the burdock fruit medicinal material according to component preparation example 6, the extracts are combined and concentrated until there is no alcohol taste, diluted with water to 7 times the equivalent volume of the concentrate, filtered, loaded with AB-8 non-polar macroporous resin, rinsed with water, and then eluted with about 70% ethanol aqueous solution, the eluate is collected, concentrated and filtered, and dried to obtain a refined burdock fruit extract.

[0110] Component Preparation Example 11: Preparation of a combined extract of Gentiana, Stephania tetrandra and Centella asiatica (Component b)

[0111] Take 1000g of plant material, including 50% gentian, 25% stephania tetrandra, and 25% centella asiatica, grind into 10 mesh coarse powder, add about 6 times of water for external circulation extraction, the extraction temperature is about 60°C, and obtain extract A1; add about 3 times of about 40% propylene glycol to the residue for external circulation extraction, the extraction temperature is about 80°C, and obtain extract A2; add about 2 times of about 70% propylene glycol to the residue for external circulation extraction, the extraction temperature is about 80°C, and obtain extract A3. Combine A1, A2, and A3, mix and pass through a 10,000 Dalton ultrafiltration membrane, collect the filtrate; filter with a nanofiltration membrane of about 100 Daltons, concentrate, and dry to obtain component b extract.

[0112] Component Preparation Example 12: Preparation of a combined extract of Gentiana, Stephania tetrandra and Centella asiatica (Component b)

[0113] Take 1000g of plant material, including 45% of Gentiana, 30% of Stephania tetrandra, and 25% of Centella asiatica, grind into 20 mesh coarse powder, add about 5 times of water for external circulation extraction, the extraction temperature is about 80°C, and obtain extract A1; add about 3 times of about 40% ethanol to the residue for external circulation extraction, the extraction temperature is about 60°C, and obtain extract A2; add about 2 times of about 70% ethanol to the residue for external circulation extraction, the extraction temperature is about 60°C, and obtain extract A3. Combine A1, A2, and A3, mix and pass through a 5000 Dalton ultrafiltration membrane, collect the filtrate; filter with a 1000 Dalton nanofiltration membrane, concentrate, and dry to obtain component b extract.

[0114] Component Preparation Example 13: Preparation of a combined extract of Gentiana scabra, Stephania tetrandra and Centella asiatica (Component b)

[0115] Take 1000g of plant material, including 60% of Gentiana, 25% of Stephania tetrandra, and 15% of Centella asiatica, grind into 20 mesh coarse powder, add about 5 times of water for external circulation extraction, the extraction temperature is about 70°C, and obtain extract A1; add about 4 times of 25% ethanol + 25% propylene glycol 1:1 mixed solvent for external circulation extraction, the extraction temperature is 70°C, and obtain extract A2; add about 3 times of 70% ethanol + 70% propylene glycol 1:1 mixed solvent for external circulation extraction, the extraction temperature is 50°C, and obtain extract A3. Combine A1, A2, and A3, mix and pass through an 8000 Dalton ultrafiltration membrane, collect the filtrate; filter with a 500 Dalton nanofiltration membrane, concentrate, and dry to obtain component b extract.

[0116] Component Preparation Example 14: Preparation of a combined extract of Gentiana scabra, Stephania tetrandra and Centella asiatica (Component b)

[0117] Take 1000g of plant material, including 40% of Gentiana, 30% of Stephania tetrandra, and 30% of Centella asiatica, grind into 20 mesh coarse powder, add about 5 times of water for external circulation extraction, the extraction temperature is about 70°C, and obtain extract A1; add about 4 times of 25% ethanol + 25% propylene glycol 1:1 mixed solvent for external circulation extraction, the extraction temperature is 70°C, and obtain extract A2; add about 3 times of 70% ethanol + 70% propylene glycol 1:1 mixed solvent for external circulation extraction, the extraction temperature is 50°C, and obtain extract A3. Combine A1, A2, and A3, mix and pass through an 8000 Dalton ultrafiltration membrane, collect the filtrate; filter with a 500 Dalton nanofiltration membrane, concentrate, and dry to obtain component b extract.

[0118] Component Preparation Example 15: Preparation of a combined extract of Gentiana, Stephania tetrandra and Centella asiatica (Component b)

[0119] Take 1000g of plant material, including 65% of Gentiana, 15% of Stephania tetrandra, and 20% of Centella asiatica, grind into 20 mesh coarse powder, add about 5 times of water for external circulation extraction, the extraction temperature is about 70°C, and obtain extract A1; add about 4 times of 25% ethanol + 25% propylene glycol 1:1 mixed solvent for external circulation extraction, the extraction temperature is 70°C, and obtain extract A2; add about 3 times of 70% ethanol + 70% propylene glycol 1:1 mixed solvent for external circulation extraction, the extraction temperature is 50°C, and obtain extract A3. Combine A1, A2, and A3, mix and pass through an 8000 Dalton ultrafiltration membrane, collect the filtrate; filter with a 500 Dalton nanofiltration membrane, concentrate, and dry to obtain component b extract.

[0120] Composition Examples

[0121] Component a prepared in Component Preparation Examples 1-10 and component b prepared in Component Preparation Examples 11-15 were mixed in different ratios to prepare the composition of the present application. Table 1 lists some of the examples and their compositions.

[0122] Table 1

[0123]

[0124]

[0125] Effect Example

[0126] Preparation of samples: Component a prepared in Preparation Example 10 and component b prepared in Preparation Example 13 were dissolved in DMSO to prepare 50 mg / mL stock solutions, and then diluted to 50 μg / mL with DMEM complete medium. Component a and component b were mixed in different ratios listed in Table 2. When components a and b were used alone at the same concentration, comparative examples 1 and 2 were obtained; when the ratio of components a and b was 30:1 and 10:1, comparative examples 3 and 4 were obtained. For ease of comparison, the compositions prepared in Examples 1-4 were selected for comparison, dissolved in DMSO to prepare 50 mg / mL stock solutions, and then diluted to 50 μg / mL with DMEM complete medium.

[0127] Table 2: Composition, proportion and concentration of the prepared samples

[0128]

[0129] 1. Cytotoxicity evaluation of burdock extract and combined extracts of gentian, stephania tetrandra and centella asiatica

[0130] 1.1 Cell culture

[0131] Human immortalized keratinocytes (HaCaT) were cultured in 90% DMEM + 10% fetal bovine serum (FBS) + 1% penicillin-streptomycin double-antibody culture medium (hereinafter referred to as DMEM complete culture medium) in a 37°C incubator containing 95% air and 5% carbon dioxide. The culture medium was replaced every two days. After the cell confluence reached 70-80%, the cells were digested with trypsin-EDTA digestion solution (0.25%) for the next experiment.

[0132] 1.2 Cytotoxicity test

[0133] HaCaT cells were cultured at 1.5 × 10 5Cells / mL were inoculated in a 96-well plate, 100 μL of cell suspension was added to each well, and the cells were cultured in a 37°C incubator for 24 hours before attachment. Components a and b were dissolved in DMSO in the sample group and diluted to the concentration to be tested with DMEM complete medium before adding to the well plate. The control group was replaced with an equal amount of complete medium and the cells were incubated for 24 hours. 10 μL of CCK-8 solution was added to each 100 μL of culture medium and placed in a cell culture incubator for 2 hours. The cytotoxicity of each sample can be determined by comparing the absorbance of the sample group and the control group at a wavelength of 450 nm.

[0134] The calculation formula is as follows: cell viability (%) = sample group OD value / control group OD value*100%. It is generally believed that when the cell viability is greater than 80%, the sample has no obvious toxicity to the cells.

[0135] Experimental results: The effect of component a (burdock extract) on HaCaT cell viability is shown in Figure 1 The results of the effect of component b (combined extracts of Gentiana, Stephania tetrandra and Centella asiatica) on the viability of HaCaT cells are shown in Figure 2 .

[0136] The experimental results show that when the mass concentration of component a (burdock extract) is 500 μg / mL or less, the survival rate of HaCaT cells is 80% or more, and when the mass concentration of component b (combined extract of gentian, stephania tetrandra and Centella asiatica) is 250 μg / mL or less, the survival rate of HaCaT cells is close to 100%, indicating that components a and b have no toxic effects on HaCaT cells within the above mass concentration range and do not affect cell growth.

[0137] 2. Evaluation of repair efficacy

[0138] 2.1 Establishment of UVB-induced barrier damage cell model

[0139] HaCaT cells were cultured at 1.5 × 10 5 Cells were inoculated at a density of 100 cells / mL in a 12-well plate, and 1 mL of cell suspension was added to each well. After culturing in a 37°C incubator for 24 hours, the cells adhered to the wall, and the culture medium in the well was discarded and replaced with 200 μL PBS. A blank group, UVB model group, and sample group were set up, with 2 replicate wells in each group. After covering the blank group with aluminum foil, the well plate was placed in a UV irradiator (VILBER LOURMAT) and irradiated with UVB at a wavelength of 312 nm at 0.15 J / cm 2 After irradiation, each well was washed twice with PBS, 1 mL of DMEM complete medium was added to each well of the blank group and UVB model group, and the corresponding samples of the embodiment and the comparative example were added to the sample group, and the culture was continued in a 37°C incubator for 3 h.

[0140] 2.2 Quantitative analysis of gene expression levels

[0141] (1) An in vitro UVB-induced barrier damage cell model was constructed according to the method in 1.2, and samples were added and cultured for 3 h according to the two group ratios in the examples and comparative examples in Table 2;

[0142] (2) RNA was extracted using the SteadyPure RNA extraction kit (ACCURATE BIOLOGY), and RNA was quantified using a NanoDrope2000 micro-volume UV spectrophotometer (Thermo Fisher).

[0143] (3) After adjusting the RNA concentration, the RNA was reverse transcribed into cDNA using the Evo M-MLV reverse transcription kit (ACCURATE BIOLOGY) for fluorescence quantitative qPCR testing.

[0144] (4) RT-qPCR test was performed using SYBR Green Pro Taq HS premixed qPCR kit (ACCURATE BIOLOGY). GAPDH was used as the internal reference gene to calculate the relative expression levels of filaggrin (FLG), zonula occludens-1 (ZO-1), and tight junction protein-1 (CLDN1) mRNA. The expression levels of the target genes in Examples 1-4 were compared with those in the UVB model group and Comparative Examples 1-4. The target gene primer sequences are shown in Table 3.

[0145] Table 3: Target gene sequences

[0146]

[0147] 2.3 Experimental results:

[0148] Depend on Figure 3-Figure 5It can be seen that after UVB irradiated HaCaT cells, the expression of cell FLG, ZO-1 and CLDN1 mRNA decreased significantly, indicating that the UVB-induced barrier damage cell model was successfully constructed. Component a (Comparative Example 1) alone only has an improvement effect on ZO-1 mRNA expression; component b (Comparative Example 2) alone has an improvement effect on FLG mRNA, but no improvement effect on ZO-1 and CLDN1 mRNA expression, indicating that component b can be used alone to regulate epidermal filament protein, thereby repairing the skin physical barrier. Component a and component b are combined in different proportions in Table 2. When the ratio range is 30:1-10:1, the expression of the target gene mRNA in Comparative Examples 3 and 4 is not significantly improved compared with the use of two components (Comparative Examples 1 and 2) alone; and when the ratio range of the two components is 4:1, 1:1, 1:4 and 1:10, Examples 1-4 have significant improvements in the expression of FLG, ZO-1 and CLDN1 mRNA (p<0.05). Other embodiments also have similar effects. Therefore, this study demonstrated that the composition of the present invention can achieve a synergistic effect on enhancing the mRNA expression levels of FLG, ZO-1 and CLDN-1 proteins, and further demonstrated that when component a (burdock extract) and component b (combined extracts of gentian, stephania tetrandra and Centella asiatica) are in the range of a compound ratio of 6:1 to 1:12, especially 4:1-1:10, they have a synergistic effect on repairing the skin's physical barrier.

[0149] It should be understood that this specification is not limited to the technical solutions shown above, and various modifications and changes can be made to various technical solutions without departing from its scope. This specification is intended to cover any modification, use or adaptive change of the present invention, which follows the general principles of this specification and includes common knowledge or common technical means in the technical field. This specification and embodiments are only regarded as exemplary, and the scope of protection of the present invention is defined by the appended claims.

Claims

1. A Chinese herbal medicine composition, wherein the composition is made from the following raw materials or contains extracts thereof: burdock fruit, gentian, stephania tetrandra and centella asiatica; preferably, the composition contains burdock fruit extract and a combined extract of gentian, stephania tetrandra and centella asiatica.

2. The Chinese herbal composition according to claim 1, wherein the weight ratio of burdock fruit or its extract to the weight ratio of the combination of gentian, stephania tetrandra and Centella asiatica or their extracts is in the range of 6:1 to 1:12, preferably in the range of 4:1 to 1:10; preferably, the combination of gentian, stephania tetrandra and Centella asiatica comprises gentian, stephania tetrandra and Centella asiatica in the following weight ratio: gentian 3-7: stephania tetrandra 1-4: Centella asiatica 1-4; more preferably, the combination of gentian, stephania tetrandra and Centella asiatica comprises gentian, stephania tetrandra and Centella asiatica in the following weight ratio: gentian 4-6.5: stephania tetrandra 1.5-3: Centella asiatica 1.5-3.

3. A method for preparing the Chinese herbal medicine composition according to claim 1 or 2, comprising the following steps: (1) weighing and crushing a burdock fruit raw material, and extracting with a solvent to obtain a burdock fruit extract; (2) weighing and crushing gentian, fangji and centella asiatica, extracting them separately or together with a solvent and then combining them to obtain a combined extract of gentian, fangji and centella asiatica; (3) mixing the extracts obtained in steps (1) and (2) to obtain the composition; Preferably, the weight ratio of burdock seeds or its extract to the weight ratio of gentian, stephania tetrandra and centella asiatica or their extracts is in the range of 6:1 to 1:12, preferably in the range of 4:1 to 1:10; more preferably, the combination of gentian, stephania tetrandra and centella asiatica comprises gentian, stephania tetrandra and centella asiatica in the following weight ratio: gentian 3-7: stephania tetrandra 1-4: centella asiatica 1-4; further preferably, the combination of gentian, stephania tetrandra and centella asiatica comprises gentian, stephania tetrandra and centella asiatica in the following weight ratio: gentian 4-6.5: stephania tetrandra 1.5-3: centella asiatica 1.5-3.

4. The method according to claim 3, wherein in step (1): the solvent is an aqueous solution of 50-90 volume % alcohol, preferably an aqueous solution of 60-80 volume % alcohol; the extraction temperature is about 60-90° C., preferably 70-80° C.; the number of extractions is 1-3 times, preferably 2 times; the duration of each extraction is 30-90 minutes, preferably 60-80 minutes; the weight ratio of the burdock seed raw material to the solvent is 1:6-1:15, preferably 1:8-1:

12.

5. The method according to claim 3 or 4, wherein step (1) further comprises a step of sieving after the pulverization; more preferably, the sieve is a 20-80 mesh sieve, more preferably a 20-40 mesh sieve.

6. The method according to any one of claims 3 to 5, wherein step (1) further comprises the step of refining the burdock fruit extract after extraction; Preferably, the step of refining the burdock fruit extract comprises concentrating the burdock fruit extract to remove the solvent, diluting it with water and filtering it, adsorbing it on a non-polar macroporous resin, and washing and eluting it with a solvent respectively to obtain a refined burdock fruit extract; Preferably, in the step of refining the burdock fruit extract, washing is performed with an aqueous solution of 0-20% by volume, preferably 0-10% by volume of alcohol; elution is performed with an aqueous solution of 50-90% by volume, preferably 70-80% by volume of alcohol; the non-polar macroporous resin is selected from D101, AB-8, HPD-100 and XDA-8, preferably AB-8; Preferably, the aqueous solution of alcohol is C 2-6 An aqueous solution of alcohol; preferably, the C 2-6 The alcohol is selected from ethanol, propanol, isopropanol, propylene glycol, n-butanol or a combination thereof, more preferably ethanol.

7. The method according to claim 3, wherein in step (2), the extraction is performed three times, and the extracts after the three extractions are mixed to obtain a combined extract of Gentiana scabra, Stephania tetrandra and Centella asiatica; the extraction comprises water extraction, a first alcohol aqueous solution extraction and a second alcohol aqueous solution extraction; Preferably, the first alcohol aqueous solution extraction uses an alcohol aqueous solution of 20-70 volume %; the second alcohol aqueous solution extraction uses an alcohol aqueous solution of 50-70 volume %; Preferably, the water extraction temperature is 60-80°C; the alcohol aqueous solution extraction temperature is 40-85°C; preferably, the alcohol aqueous solution is C 2-6 An aqueous solution of alcohol; preferably, the C 2-6 The alcohol is selected from ethanol, propanol, propylene glycol, n-butanol or a combination thereof, more preferably propylene glycol and ethanol.

8. Use of the Chinese herbal composition according to claim 1 or 2 or the Chinese herbal composition prepared according to the method according to any one of claims 3 to 7 in the preparation of a medicine or cosmetic for repairing the skin barrier.

9. Use of a combined extract of gentian, fangji and centella asiatica in the preparation of a medicine or cosmetic for repairing the skin barrier; Preferably, the combined extract is made from or comprises extracts of Gentiana, Stephania Tetrandra and Centella asiatica in the following weight ratio: Gentiana 3-7: Stephania Tetrandra 1-4: Centella asiatica 1-4, more preferably Gentiana 4-6.5: Stephania Tetrandra 1.5-3: Centella asiatica 1.5-3.

10. A medicine or cosmetic, wherein the medicine or cosmetic comprises the Chinese herbal composition according to claim 1 or 2, or the Chinese herbal composition prepared according to the method of any one of claims 3 to 7, or the combined extract of Gentiana scabra, Stephania tetrandra and Centella asiatica as an active ingredient, and one or more pharmaceutically or cosmetically acceptable carriers, diluents or excipients; Preferably, the Chinese herbal composition accounts for 0.1-10% by weight of the medicine or cosmetic, preferably 0.1-2%, more preferably 0.1-0.5% by weight.

11. The drug or cosmetic according to claim 10, wherein the drug or cosmetic is a cream, an emulsion, a cream, an ointment, a gel, a lotion or an essence.

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