Tibetan veterinary medicine composition for treating weaned yak spleen deficiency type diarrhea

Abstract

The invention discloses a Tibetan veterinary drug composition for treating weaned yak spleen deficiency type diarrhea, which takes delphinium monoflower, caraway and rhizoma nardostachyos as monarch drugs, allium chrysanthum and radix aconiti brachypodi (processed) as ministerial drugs, polygonum capitatum and daphne ovata as adjuvant drugs, and sludge element as a conductant drug. The delphinium uniflower can warm intestines and stomach and stop diarrhea and dysentery; the caraway can warm spleen and stomach for dispelling cold and strengthen spleen and stomach; the rhizoma nardostachyos can warm stomach and invigorate spleen; radix aconiti brachypodi (processed) has the effects of dispelling cold, removing dampness, dispersing blood stasis and relieving pain; the allium chrysanthum can tonify spleen, promote appetite, promote digestion Polygonum capitatum is used for cold accumulation, long dysentery, regurgitation and vomiting; the daphne odera has the effects of calming endogenous wind, clearing heat, inducing diuresis and diminishing The sludge element astringes to stop diarrhea and is used for enteritis, dysentery and The safe and efficient Tibetan veterinary drug composition is formed on the basis of the synergistic effect characteristics of multiple components, multiple ways and multiple targets, and can effectively prevent and treat diarrhea of weaned yaks caused by cold evil such as deficiency-cold in spleen and stomach. The immunity and the production performance are improved; meanwhile, the morbidity and the mortality of weaned yaks caused by diarrhea are reduced.

Description

Technical Field

[0001] The invention relates to the technical field of veterinary medicines, and in particular to a Tibetan veterinary medicine composition for treating spleen deficiency type diarrhea in weaned yaks. Background Art

[0002] Yaks are the main source of income in high-altitude and cold regions, and yak breeding is extremely important for agricultural and pastoral areas. Diarrhea is one of the main diseases that plague the normal growth of yaks. Breeders should attach great importance to it, do a good job in the prevention and treatment of diarrhea, and ensure the healthy growth of yaks. After birth, yaks are prone to low immunity and digestive system disorders, which makes yaks vulnerable to external factors and cause diseases. Young yaks need to obtain the nutrients needed for growth from breast milk to ensure the growth of the body and the improvement of immunity. If the number of immunoglobulins in milk is low, the yak's own immunity will not be improved. Once infected, diarrhea will occur. After weaning, due to the immature development of various digestive organs and the lack of a complete digestive system, its digestive ability is weak. Even if excessive milk is consumed, it will increase the burden on the intestines. The milk that cannot be absorbed in time in the intestines will continue to ferment and cause diarrhea.

[0003] Diarrhea due to deficiency of spleen and stomach in weaned yaks is a common and frequently occurring disease, which is mainly manifested by persistent diarrhea, dehydration, slow production and high mortality in young weaned yaks, seriously affecting the healthy and sustainable development of yak breeding. At present, the prevention and treatment of diarrhea due to deficiency of spleen and stomach in weaned yaks mainly rely on the intervention of drugs, especially chemical drugs and herbal drugs, but both have certain shortcomings and deficiencies. For example, although the single use of herbal drugs such as traditional Chinese medicine has good safety and effectiveness, the preparation process is complicated, the ingredients are unstable, the administration method is inconvenient, the acceptance is low, the efficacy is slow, and it is difficult to meet emergency needs; although chemical drugs have faster efficacy and higher acceptance, they have large side effects and are prone to drug resistance, affecting spleen and stomach function and increasing drug residues, endangering the health and safety of weaned yaks while affecting food safety.

[0004] Based on the above analysis, the art urgently needs a new method for preventing and treating yak spleen and stomach deficiency and coldness, and provides a safe, effective, convenient and economical prevention and treatment preparation to solve a series of problems caused by spleen and stomach deficiency and coldness diarrhea in weaned yaks. Summary of the invention

[0005] In view of the above-mentioned deficiencies, the present invention provides a Tibetan veterinary medicine composition for preventing and treating diarrhea caused by deficiency-coldness of the spleen and stomach in yaks, which is clinically verified to promote its application and provide data support.

[0006] The present invention is achieved by the following technical means:

[0007] The present invention first discloses a Tibetan veterinary medicine composition for treating spleen deficiency diarrhea in weaned yaks, comprising:

[0008] 25-35 parts of single-flowered delphinium flowers, 10-30 parts of caraway, 10-15 parts of spoon-leaved spikenard, 15-25 parts of wild onion, 10-15 parts of polygonum capitata, 10-20 parts of small drooping chrysanthemum, 10-15 parts of snow wormwood (processed), and 25-40 parts of sludge element.

[0009] Furthermore, the Tibetan veterinary medicine composition comprises:

[0010] 25 parts of single flower delphinium flowers, 20 parts of caraway, 15 parts of spoon-leaved spikenard, 25 parts of wild onions, 10 parts of polygonum capitata, 15 parts of small drooping chrysanthemums, 15 parts of artemisia annua (processed), 40 parts of sludge element.

[0011] Furthermore, the Artemisia sibiricum (processed) is prepared by the following processing method:

[0012] First, fry the coarse sand in an iron pan, then put the collected fresh roots of Artemisia snowdrops into the hot sand. After the roots turn slightly yellow, crush them in a grinder and pass through an 80-mesh pharmacopoeia sieve to obtain Artemisia snowdrops (processed).

[0013] Furthermore, the sludge is prepared by the following method:

[0014] The soil collected from the wetland in Hongyuan County was naturally air-dried, sieved to remove impurities, and 4 times the weight of sterile water was added to dissolve the soil. After sufficient homogenization, the soil was allowed to stand for 2-4 hours, and the upper liquid was removed. Then 4 times the weight of sterile water was added to dissolve the soil. After sufficient homogenization, the soil was allowed to stand for 2-4 hours, and the upper liquid was removed. Finally, the lower layer of soil was dried, crushed, and sieved through a 200-mesh pharmacopoeia sieve to obtain sludge.

[0015] The present invention also discloses a method for preparing a Tibetan veterinary medicine composition for treating spleen deficiency type diarrhea in weaned yaks, comprising:

[0016] (1) Weigh Artemisia sibiricum (processed), add 150 mL of deionized water in a ratio of 1:10, boil in boiling water for 40 minutes, and filter to obtain the first filtrate for later use;

[0017] (2) Weigh delphinium uniflorum, caraway, spoon-leaved nard, wild onion, polygonum capitatum, and chrysanthemum officinale respectively, add 10 times the weight of deionized water of the total weight of the drugs and soak for 1 hour, boil over high heat in a ceramic Chinese medicine pot until boiling, then continue to boil over low heat for 30 minutes, filter the liquid, add 5 times the weight of deionized water to the filtered liquid and boil again once, and obtain the second filtrate for use;

[0018] (3) The first filtrate and the second filtrate were combined, and the sludge was added. After mixing, the mixture was boiled until boiling, and then the mixture was boiled over low heat for 20 minutes. After filtering, the filtrate was concentrated to a certain volume on a rotary evaporator. Anhydrous ethanol was added to the concentrated solution by volume until the final alcohol content was 75%. After standing overnight, the supernatant was filtered out, and the ethanol was recovered by a rotary evaporator until there was no alcohol taste. The solution was concentrated to a final concentration of 1g / mL. 1mol / L sodium hydroxide and 1mol / L hydrochloric acid solution were used to adjust the pH value to 7.4. Cosolvents, flavoring agents and preservatives were added according to the volume ratio to a final concentration of 0.3%, 0.4% and 0.5%, respectively. Finally, the solution was divided into 100mL polypropylene plastic bottles and sterilized at 100℃ for 15 minutes for use.

[0019] Furthermore, the cosolvent is Tween-80, the flavoring agent is steviol glycoside, and the preservative is sodium benzoate.

[0020] The invention also discloses a medicine, comprising the Tibetan veterinary medicine composition.

[0021] Furthermore, the dosage form of the drug is an oral preparation.

[0022] Furthermore, the oral preparation is an oral solution.

[0023] The present invention also discloses an application of any of the above Tibetan veterinary medicine compositions or any of the above medicines in preparing a method for treating spleen deficiency type diarrhea in weaned yaks.

[0024] Furthermore, the use of the drug in the preparation of a medicine for treating spleen deficiency-type diarrhea in weaned yaks includes:

[0025] According to the dosage of crude drug for oral solution preparation, the dosage for weaned yaks is 120 mg kg. -1 , 2 times / d, for 5 consecutive days.

[0026] The beneficial effects of the present invention are:

[0027] The implementation of this case can not only effectively prevent and treat diarrhea caused by spleen deficiency in weaned yaks, especially diarrhea caused by cold deficiency, reduce the morbidity and mortality of weaned yaks, but also play a certain role in enhancing the disease resistance and production performance of young weaned yaks, and can guide farmers and herdsmen to rationally use the medicinal plant resources in the Tibetan area of ​​the northwest Sichuan plateau to prevent and treat common diseases represented by diarrhea. In addition, it also has a certain role in promoting the inheritance, protection and innovative development of Tibetan veterinary medicine culture. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail with reference to the following embodiments.

[0029] 1. Materials

[0030] 1.1 Collection and processing of medicinal materials

[0031] Delphinium candelabrum var.monanthum(Hand.-Mazz.)WTWang), Carum carvi L., Nardostachys chinensis Batal., Alliumchrysanthum Regel, Polygonum capitatum Buch.-Ham.ex D.Don, Cremanthodium nanum(Decnc.)WWSmith and other medicinal materials were collected from Ganzi Tibetan Autonomous Prefecture and Aba Tibetan and Qiang Autonomous Prefecture from July 2023 to March 2024. After collection, the soil on the roots was washed, and the dead branches and rotten leaves were removed. After natural air-drying in a ventilated place, they were crushed and passed through an 80-mesh pharmacopoeia sieve for later use.

[0032] Preparation method of Aconitum szechenyianum Gay: First, fry the coarse sand in an iron pan, then put the collected fresh roots into the hot sand. After the roots turn slightly yellow, grind them in a grinder and pass through an 80-mesh pharmacopoeia sieve for later use.

[0033] Preparation of sludge: The soil collected from the wetland in Hongyuan County was naturally air-dried, sieved to remove impurities, weighed, and 4 times the weight of sterile water was added to dissolve the soil. After sufficient homogenization, it was allowed to stand for 2-4 hours, and the upper liquid was removed; then 4 times the weight of sterile water was added to repeat the above steps, and finally the lower layer of soil was dried, crushed and passed through a 200-mesh pharmacopoeia sieve for later use.

[0034] 2. Methods

[0035] 2.1 Prescription ratio and its screening

[0036] Weigh 10-15 portions of Artemisia sibiricum (processed), add deionized water in a ratio of 1:10, boil in boiling water for 40 minutes and filter;

[0037] Weigh 25-35 parts of Delphinium uniflorum, 10-30 parts of Caraway, 10-15 parts of Nardostachys spathulata, 15-25 parts of Allium campestris, 10-15 parts of Polygonum capitatum, and 10-20 parts of Chrysanthemum sphaerocephalum respectively, add deionized water in a ratio of 1:10, soak for 1 hour, boil over high heat in a ceramic Chinese medicine pot until boiling, then change to low heat and continue boiling for 30 minutes. After filtering the medicinal liquid, add deionized water in a ratio of 1:5 and repeat the above steps for 1 time.

[0038] After combining the two filtrates, combine the filtrates of Artemisia sibiricum, add 25-40 parts of sludge, mix and boil until boiling, then change to slow fire and continue to boil for 20 minutes. After filtering, add the filtrate to a certain volume in a rotary evaporator, add anhydrous ethanol to the concentrated liquid by volume until the final alcohol content is 75%, stand overnight and filter out the supernatant, recycle the ethanol on the rotary evaporator until there is no alcohol taste and concentrate the liquid to a final concentration of 1g / mL, adjust the pH value to about 7.2-7.4 with 1mol / L sodium hydroxide and 1mol / L hydrochloric acid solution, add Tween-80 (solvent), stevioside (flavor corrective) and sodium benzoate (preservative) according to the volume ratio to the final concentration of 0.3%, 0.4% and 0.5%, respectively. Finally, the liquid is divided into 100mL polypropylene plastic bottles and sterilized at 100℃ for 15min for standby use.

[0039] During the experiment, the medicinal materials of each prescription were used to prepare the combination according to different proportions, and the best compatibility ratio was screened to prepare the combined oral liquid.

[0040] Take implementation case 1 as an example to conduct the following test:

[0041] Example 1

[0042] The best proportion of the composition and the principle of compatibility of monarch, minister, assistant and envoy:

[0043] According to the results of the implementation case, the optimal formula ratio was determined to be: 25:20:15:25:10:15:15:40, namely: 25 parts of single-flowered delphinium, 20 parts of fennel, 15 parts of spatholobus, 25 parts of wild onion, 10 parts of Polygonum capitatum, 15 parts of small drooping chrysanthemum, 15 parts of Artemisia suspensa (processed), and 40 parts of sludge. In the formula, single-flowered delphinium, fennel, and spatholobus are the main drugs, wild onion and Artemisia suspensa (processed) are the ministerial drugs, Polygonum capitatum and small drooping chrysanthemum are the adjuvant drugs, and sludge is the guiding drug. The single-flowered Delphinium flower in the Tibetan veterinary medicine composition and oral liquid warms the stomach and intestines and stops diarrhea; the Tibetan fennel warms the middle and dispels cold, strengthens the spleen and stomach; the spoon-leaved Nardostachys chinensis warms the stomach and strengthens the spleen; the Artemisia selengensis (processed) dispels cold and dampness, dissipates blood stasis and relieves pain; the wild onion strengthens the spleen and stimulates appetite, aids digestion, etc.; the Polygonum capitatum is used for cold accumulation and chronic diarrhea, nausea and vomiting; the small drooping chrysanthemum calms the wind and relieves heat, promotes diuresis and reduces swelling; the sludge element is astringent and stops diarrhea, and is used for enteritis and dysentery, etc.

[0044] Preparation process of oral liquid composition:

[0045] Weigh 15 portions of Artemisia sibiricum (processed), add 150 mL of deionized water at a ratio of 1:10, boil in boiling water for 40 minutes, filter and set aside;

[0046] Weigh 40g of naturally air-dried wetland soil, sieve it to remove impurities, weigh it, add 160mL of sterile water to dissolve the soil, fully homogenize it and let it stand for 2-4h, remove the upper liquid; then add 160mL of sterile water and repeat the above steps. Finally, dry the lower soil, crush it and pass it through a 200-mesh pharmacopoeia sieve to prepare sludge for use.

[0047] Weigh 25 parts of Delphinium uniflorum, 20 parts of Caraway, 15 parts of Nardostachys chinensis, 25 parts of Allium wilfordii, 10 parts of Polygonum capitatum, and 15 parts of Cymbidium sp. respectively, add deionized water in a ratio of 1:10, soak for 1 hour, boil over high heat in a ceramic Chinese medicine pot until boiling, then change to low heat and continue boiling for 30 minutes. After filtering the medicinal liquid, add deionized water in a ratio of 1:5 and repeat the above steps for 1 time.

[0048] After combining the two filtrates, combine the filtrates of Artemisia sibiricum, add 25 parts of sludge, mix and boil until boiling, then change to slow fire and continue to boil for 20 minutes. After filtering, add the filtrate to a certain volume in a rotary evaporator, add anhydrous ethanol to the concentrated liquid by volume until the final alcohol content is 75%, let it stand overnight, filter out the supernatant, recycle the ethanol on the rotary evaporator until there is no alcohol taste, and concentrate the liquid to a final concentration of 1g / mL, adjust the pH value to 7.4 with 1mol / L sodium hydroxide and 1mol / L hydrochloric acid solution, add Tween-80 (solvent), stevioside (flavor corrective) and sodium benzoate (preservative) according to the volume ratio to the final concentration of 0.3%, 0.4% and 0.5%, respectively. Finally, the liquid is divided into 100mL polypropylene plastic bottles and sterilized at 100℃ for 15min for standby use.

[0049] 2.2 Acute toxicity test of oral solution of combined composition—modified Koch method

[0050] First, a preliminary test was conducted on the experimental animals to explore the experimental dose range and find out the estimated lethal dose that causes 0-100%. Then, a formal test was conducted: 50 mice weighing 20±2g, half male and half female, were randomly divided into 10 groups, 5 mice / group. Kunming mice were randomly divided into groups, 5 mice in each group, and after fasting for 16 hours, they were gavaged with 500-5000mg·Kg-1 of the test drug in the dose gradient of 500mg·Kg-1. The control group was given an equal amount of physiological saline, and the reaction of the mice within 24 hours was observed and recorded to make a preliminary evaluation of acute toxicity. Statistical calculations were performed according to the modified Coulthard method based on the survival of the animals.

[0051] 2.3 Evaluation of the taste of oral liquid composition - Determination of preference index

[0052] The preference index, also known as the preference degree (TGI, Targeted Group Index), is mainly used to measure the difference between the attention degree of the target group under a specific feature and the overall level. This study used a two-bottle preference test method to evaluate the mice's preference for experimental oral solution and water: before the experiment, the mice were trained to drink water to adapt to the environment and become familiar with drinking water. In the formal experimental stage, the mice were placed in the experimental device and given oral solution of experimental drugs and pure water at the same time. During the experiment, the drinking behavior data of the mice were recorded in real time and the oral solution preference index (oral solution consumption / total water intake × 100%, calculated as a percentage) was calculated. In the experiment, there were 20 Kunming mice in each oral solution group and pure water group, each group was divided into 5 cages, and each had 4 replicates.

[0053] 2.4 Verification of the Rhubarb + Senna Spleen Deficiency Diarrhea Model in Mice - Spleen Strengthening Effect

[0054] 42 BAB / c mice (18±2g) were randomly divided into 6 groups. After acclimation for 3 days, except for the negative control group which was given normal saline, the other groups were gavaged with rhubarb + senna leaf decoction (0.5g+0.5g / 10g) for 8 consecutive days to replicate the spleen deficiency diarrhea model. The weight of mice in each group was recorded after the last gavage. The mice were fasted for 16 hours before the experiment. The high, medium and low drug dose groups were given 720, 600 and 500 mg·Kg respectively. -1 (Calculated as crude drug), the model group and negative control group were given normal saline, and the positive control group was given 60 mg·Kg -1 Guipi Pills were administered once a day for 7 consecutive days in each group. The weight of mice was recorded again before the last administration. 1 hour after the last administration, each mouse was given 0.2 mL of ink, and 30 minutes later, the mouse was killed by dislocation. The abdominal cavity was quickly opened and the small intestine, thymus and spleen were taken to calculate the intestinal propulsion rate, thymus index and spleen index. During the experiment, the diarrhea of ​​mice in each group was recorded at the same time, including the loose stool rate, loose stool grade and diarrhea index.

[0055] 2.5 Evaluation of clinical effects of anti-diarrhea

[0056] 2.5.1 Clinical treatment trial of weaned yaks

[0057] 49 weaned yaks with diarrhea were selected and randomly divided into a test group (25 yaks) and a control group (24 yaks). The combination oral solution and the control drug were used for treatment respectively. Dosage regimen: combination oral solution (120 mg·Kg -1 , calculated according to the dosage of crude drug for oral solution preparation), 2 times / d, for 5 consecutive days; Guipi Pills (60mg·Kg -1 ), 2 times / d, for 5 consecutive days.

[0058] 2.5.2 Clinical prevention trial in weaned yaks

[0059] 47 weaned yaks with diarrhea were selected and randomly divided into a test group (24 yaks) and a control group (23 yaks). The oral solution of the composition and normal saline were used respectively. Dosage regimen: oral solution of the composition (120 mg·Kg -1 ,, calculated according to the dosage of crude drug for oral solution preparation), 2 times / d, for 5 consecutive days; the control group was given normal saline. Observe whether diarrhea occurs during medication and within 3 days after stopping medication, and calculate the protection rate.

[0060] 2.5.3 Growth promotion test

[0061] 54 healthy weaned yaks were selected and randomly divided into an experimental group (27 yaks) and a blank control group (27 yaks). The dosing regimen of the experimental group was the same as 2.5.1, and it was used for 21 days; no drug was added to the blank control group. The incidence of diarrhea in weaned yaks during the medication period was observed, and the feed intake and body weight on the weaning day, 7th day, 14th day and 21st day after weaning were recorded.

[0062] Experimental weaning day, weaning

[0063] 3. Test results

[0064] 3.1 Optimal ratio of the composition and the principle of compatibility of monarch, minister, assistant and envoy

[0065] According to the results of the implementation case, the optimal formula ratio was determined to be: 25:20:15:25:10:15:15:40, namely: 25 parts of single-flowered delphinium, 20 parts of fennel, 15 parts of spatholobus, 25 parts of wild onion, 10 parts of Polygonum capitatum, 15 parts of small drooping chrysanthemum, 15 parts of Artemisia suspensa (processed), and 40 parts of sludge. In the formula, single-flowered delphinium, fennel, and spatholobus are the main drugs, wild onion and Artemisia suspensa (processed) are the ministerial drugs, Polygonum capitatum and small drooping chrysanthemum are the adjuvant drugs, and sludge is the guiding drug. The single-flowered Delphinium flower in the Tibetan veterinary medicine composition and oral liquid warms the stomach and intestines and stops diarrhea; the Tibetan fennel warms the middle and dispels cold, strengthens the spleen and stomach; the spoon-leaved Nardostachys chinensis warms the stomach and strengthens the spleen; the Artemisia selengensis (processed) dispels cold and dampness, dissipates blood stasis and relieves pain; the wild onion strengthens the spleen and stimulates appetite, aids digestion, etc.; the Polygonum capitatum is used for cold accumulation and chronic diarrhea, nausea and vomiting; the small drooping chrysanthemum calms the wind and relieves heat, promotes diuresis and reduces swelling; the sludge element is astringent and stops diarrhea, and is used for enteritis and dysentery, etc.

[0066] 3.2 Preparation of oral solution

[0067] According to the implementation case 1 in the preparation process of oral solution 2.1, the composition is added to the deionized water in a ratio of 1:10, soaked for 1 hour, and then decocted in a ceramic Chinese medicine pot with high heat until boiling, and then decocted for 30 minutes with low heat. After filtering the liquid, deionized water is added in a ratio of 1:5 and the above steps are repeated for 1 time. After filtering, the filtrate is concentrated to a certain volume in a rotary evaporator, and anhydrous ethanol is added to the concentrated liquid by volume until the final alcohol content is 75%. After standing overnight, the supernatant is filtered out, and the ethanol is recovered by a rotary evaporator until there is no alcohol taste and the liquid is concentrated to a final concentration of 1g / mL. 1mol / L sodium hydroxide and 1mol / L hydrochloric acid solution are used to adjust the pH value to 7.4, and Tween-80 (cosolvent), stevioside (flavor corrector) and sodium benzoate (preservative) are added according to the volume ratio to a final concentration of 0.3%, 0.4% and 0.5%, respectively. Finally, the drug solution was divided into 100 mL polypropylene plastic bottles and sterilized under high pressure at 100°C for 15 min for later use.

[0068] 3.2 Evaluation of acute toxicity of oral solution

[0069] Acute toxicity tests showed that mice in each experimental group did not show normal behavioral changes or death, and the LD50 of the oral solution could not be measured, which preliminarily confirmed that the screened composition and oral solution were safe and non-toxic.

[0070] 3.3 Oral liquid taste evaluation - TGI index

[0071] The two-bottle preference test method was used to evaluate the preference of mice for the experimental oral solution and water. The experimental results are shown in Table 1. The oral solution preference index was 1.08 (31.87% / 29.39%), indicating that the oral solution had good palatability, no peculiar smell, and was highly accepted by mice.

[0072] Table 1 Determination of TGI index in mice with spleen deficiency diarrhea caused by rhubarb + senna

[0073]

[0074] 3.4 Verification of the Rhubarb + Senna Spleen Deficiency Diarrhea Model in Mice

[0075] 3.4.1 Effect of oral solution on body weight of mice with spleen deficiency diarrhea caused by rhubarb + senna

[0076] Compared with the blank group, after the successful modeling of spleen deficiency diarrhea with rhubarb + senna, the body weight of mice in the model group and the experimental group was lower than that in the blank group. The mice in the experimental group had fluffy and dull fur, reduced food intake, dry and hard stools, reduced activity, fatigue, limb weakness, weight loss and other symptoms. After administration, there was no significant difference in the positive group compared with the blank group, indicating that the control drug Guipi Pills can effectively improve the body weight of mice with spleen deficiency, while the body weight of mice in the drug administration group was lower than that in the blank group, and there was a significant difference. Compared with the model group, there was no significant difference in the oral liquid dosage groups, indicating that the oral liquid had no significant effect on the weight recovery of mice with spleen deficiency diarrhea with rhubarb + senna.

[0077] Table 2 Effect of oral liquid on body weight of mice with spleen deficiency diarrhea caused by rhubarb + senna

[0078] Group <![CDATA[Dose (mg·Kg -1 )]]> Before modeling(g) After modeling (g) After administration (g) Blank Group - 18.71±0.89 20.08±0.74## 22.30±0.47# Model Group - 19.05±0.44 20.75±0.72** 22.91±1.06* Guipi Pills Group 60 19.16±0.31 21.92±0.91* 22.45±1.00 Low dose group 500 19.47±0.42 20.04±0.89** 21.31±0.97* Medium dose group 600 20.05±0.36 21.08±0.87** 21.70±1.03** High dose group 720 19.17±1.01 21.14±1.27 22.15±0.63*

[0079] Note: Compared with the blank group, * indicates P < 0.05, ** indicates P < 0.01; compared with the model group, # indicates P < 0.05, ## indicates P < 0.01; the same below.

[0080] 3.4.3 Effect of oral solution on diarrhea in mice with spleen deficiency diarrhea caused by rhubarb + senna

[0081] During the experiment, the diarrhea conditions of mice in each group were recorded, including loose stool rate, loose stool grade and diarrhea index. The results are shown in Table 3.

[0082] Table 3 Effect of oral liquid of the combination on diarrhea in mice with spleen deficiency diarrhea caused by rhubarb + senna

[0083] Group quantity <![CDATA[Dose (mg·Kg -1 )]]> Loose stool rate Loose stool Diarrhea Index Blank Group 7 - 0.23±0.10 2.01±0.52 0.46±0.20 Model Group 7 - 0.27±0.13 3.20±0.39 0.86±0.34 Guipi Pills Group 7 60 0.21±0.16 2.71±0.64 0.57±0.31 Low dose group 7 500 0.24±0.11 2.34±0.43 0.56±0.26 Medium dose group 7 600 0.21±0.14 1.94±0.61 0.41±0.17 High dose group 7 720 0.27±0.11 1.77±0.53 0.48±0.20

[0084] 3.4.4 Effect of combined oral liquid on intestinal propulsion rate in mice with spleen deficiency diarrhea caused by rhubarb + senna

[0085] The test results are shown in Table 4: The intestinal propulsion rate of mice in the model group was lower than that in the blank group, indicating that mice with spleen deficiency can slow down defecation. Compared with the model group, the intestinal propulsion rate of mice in the positive group and the low-dose oral liquid group of the combination increased, among which the positive group had a significant difference, while the intestinal propulsion rate of the medium-dose and high-dose oral liquid groups of the combination was slightly lower than that of the model group, and there was no statistical significance, indicating that the oral liquid of the combination had little effect on the intestinal propulsion rate of mice with spleen deficiency.

[0086] Table 4 Effect of oral liquid of the combination on intestinal propulsion rate of spleen deficiency model in mice induced by rhubarb + senna leaves

[0087] Group quantity <![CDATA[Dose (mg·Kg -1 )]]> Advancement rate (%) Blank Group 7 - 71.23±3.10 Model Group 7 - 62.27±6.13 Guipi Pills Group 7 60 73.21±5.16# Low dose group 7 500 70.24±6.11 Medium dose group 7 600 61.21±6.14 High dose group 7 720 62.27±5.13

[0088] 3.4.5 Effect of oral liquid of combination on thymus index and spleen index of mice with spleen deficiency diarrhea caused by rhubarb + senna

[0089] After administration, the thymus index and spleen index of the model group mice were lower than those of the blank group and the difference was significant. Compared with the model group, the thymus index and spleen index of the low-dose oral liquid group of the combination increased to varying degrees, among which the thymus index had a significant difference, but did not return to the normal level of the blank group, while the thymus index and spleen index of the medium and high-dose groups were lower than those of the model group, indicating that the oral liquid of the combination had a certain effect on the thymus index and spleen index of spleen-deficient mice, and there was no obvious dose-dependent effect (Table 5).

[0090] Table 5 Effect of oral liquid of the combination on thymus index and spleen index of spleen deficiency model of mice induced by rhubarb + senna

[0091] Group <![CDATA[Dose (mg·Kg -1 )]]> Thymus index (mg / g) Spleen index (mg / g) Blank Group - 2.53±0.41## 4.31±0.65# Model Group - 1.61±0.43** 3.17±1.01* Guipi Pills Group 60 2.54±0.18## 4.37±0.91## Low dose group 500 2.37±0.63# 3.48±0.92 Medium dose group 600 1.65±0.67** 2.75±0.71** High dose group 720 1.84±0.65** 2.79±0.98**

[0092] 3.5 Clinical verification and effect evaluation of the effect of spleen deficiency and cold diarrhea in weaned yaks

[0093] 3.5.1 Clinical treatment trial of weaned yaks

[0094] The clinical treatment results of the oral liquid of the combination on weaned yaks are shown in Table 6. The effects on the loose stool rate, loose stool grade and diarrhea index are shown in Table 7, from which it can be seen that although the loose stool grade of the oral liquid group of the combination is higher than that of the Guipi Pills group, the loose stool rate and diarrhea index are lower than those of the control group, and the overall treatment effect is not significantly different from that of the Guipi Pills group.

[0095] Table 6 Clinical treatment results of oral liquid of the composition on weaned yaks

[0096]

[0097] Table 7 Effects of oral liquid and Guipi pills on diarrhea in weaned yaks

[0098] Group quantity Loose stool rate Loose stool Diarrhea Index Combination group 25 0.17±0.04 3.07±0.43 0.52±0.11 Guipi Pills Group 24 0.21±0.06 3.41±0.26 0.72±0.23

[0099] 3.5.2 Clinical prevention trial in weaned yaks

[0100] As can be seen from Table 8, the oral liquid of the combination has a preventive and therapeutic effect of 78.26%. The effects on the loose stool rate, loose stool grade and diarrhea index are shown in Table 9, from which it can be seen that the loose stool rate, loose stool grade and diarrhea index of the oral liquid of the combination are lower than those of the control group.

[0101] Table 8 Clinical prevention results of oral liquid of the composition on weaned yaks

[0102] Group quantity Number of cases (head) Prevention and protection rate (%) Combination group 23 5 78.26 Normal saline group 24 17 29.17

[0103] Table 9 Effects of oral liquid and Guipi pills on diarrhea in weaned yaks

[0104] Group quantity Loose stool rate Loose stool Diarrhea Index Combination group 23 0.31±0.21 2.21±0.37 0.69±0.43 Normal saline group 24 0.33±0.13 2.24±0.43 0.74±0.35

[0105] 3.5.3 Growth promotion test

[0106] During the medication period, the feed intake and body weight of weaned yaks on the experimental weaning day, 7th day, 14th day and 21st day after weaning are shown in Table 10, from which it can be seen that the average daily weight gain of the oral liquid of the composition is higher than that of the control group.

[0107] Table 10 Growth-promoting effect of oral liquid of the composition on weaned yaks (unit: kg)

[0108]

[0109]

[0110] Note: “-” means no drug is added.

[0111] The above results show that the oral liquid preparation of the composition of the present invention can effectively wean yaks from diarrheal diseases and improve their feed intake and growth performance, and has significant superiority and creativity.

[0112] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Tibetan veterinary medicine composition for treating spleen deficiency diarrhea in weaned yaks, comprising: 25-35 parts of single-flowered delphinium flowers, 10-30 parts of caraway, 10-15 parts of spoon-leaved spikenard, 15-25 parts of wild onion, 10-15 parts of polygonum capitata, 10-20 parts of small drooping chrysanthemum, 10-15 parts of snow wormwood (processed), and 25-40 parts of sludge element.

2. The Tibetan veterinary medicine composition according to claim 1, wherein: 25 parts of single flower delphinium flowers, 20 parts of caraway, 15 parts of spoon-leaved spikenard, 25 parts of wild onions, 10 parts of polygonum capitata, 15 parts of small drooping chrysanthemums, 15 parts of artemisia annua (processed), 40 parts of sludge element.

3. The Tibetan veterinary medicine composition according to claim 1 or 2, wherein: The sludge element is prepared by the following method: The soil collected from the wetland in Hongyuan County was naturally air-dried, sieved to remove impurities, and 4 times the weight of sterile water was added to dissolve the soil. After sufficient homogenization, the soil was allowed to stand for 2-4 hours, and the upper liquid was removed. Then 4 times the weight of sterile water was added to dissolve the soil. After sufficient homogenization, the soil was allowed to stand for 2-4 hours, and the upper liquid was removed. Finally, the lower layer of soil was dried, crushed, and sieved through a 200-mesh pharmacopoeia sieve to obtain sludge.

4. A method for preparing a Tibetan veterinary medicine composition for treating spleen deficiency diarrhea in weaned yaks according to any one of claims 1 to 3, comprising: (1) Accurately weigh Artemisia sibiricum (processed), add 10 times the amount of deionized water and boil for 40 minutes, and filter to obtain the first filtrate for later use; (2) Weigh delphinium uniflorum, caraway, spoon-leaved nard, wild onion, polygonum capitatum, and chrysanthemum officinale respectively, add 10 times the weight of deionized water to soak for 1 hour, decoct for 30 minutes, filter the drug solution, add 5 times the weight of deionized water to the filtered drug solution, and decoct again once to obtain a second filtrate for later use; (3) The first filtrate and the second filtrate are combined, sludge is added, and the mixture is boiled until boiling, then the mixture is boiled over low heat for 20 minutes, and the third filtrate is obtained after filtering; (4) The third filtrate is concentrated, and anhydrous ethanol is added to a final alcohol content of 75%. After standing, the supernatant is collected overnight, and the supernatant is concentrated to a final concentration of 1 g / mL by a rotary evaporator. The pH value is adjusted to 7.4, and then a cosolvent, a flavoring agent, and a preservative are added to a final concentration of 0.3%, 0.4%, and 0.5%, respectively. The product is packaged and sterilized.

5. The preparation method according to claim 4, wherein: The cosolvent in step (4) is Tween-80, the flavoring agent is steviol glycoside, and the preservative is sodium benzoate.

6. A Tibetan veterinary medicine composition prepared according to the preparation method according to claim 4 or 5.

7. A medicine comprising the Tibetan veterinary medicine composition according to any one of claims 1 to 3 or 6.

8. The drug according to claim 7, wherein: The dosage form of the drug is an oral solution.

9. Use of the Tibetan veterinary medicine composition according to any one of claims 1 to 3 and 6 or the medicine according to claim 7 or 8 in the preparation of a medicine for treating spleen deficiency diarrhea in weaned yaks.

10. The use according to claim 9, wherein: The use of the medicine in preparing the treatment of spleen deficiency diarrhea in weaned yaks includes: According to the dosage of crude drug for preparing oral solution, the dosage for weaned yaks is 120 mg kg. -1 , 2 times / d, for 5 consecutive days.

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