Primer group, detection method, kit and application for identifying coilia ectenes, coilia brachygnathus and hybrids of coilia ectenes and coilia brachygnathus
By designing primer sets for identification of snails, short-jawed snails and hybrids, and combining with agarose gel electrophoresis detection methods, the problems of difficulty and high cost in the prior art are solved, and a fast, accurate and low-cost identification effect is achieved.
CN119639917AInactive Publication Date: 2025-03-18九江市农业科学院 +1
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Patent Information
- Application Number
- CN202411889132.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-03-18
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Technical Problem
It is difficult to effectively identify slashes, short-jawed slashes and their hybrids in the prior art. Traditional morphological methods are affected by environmental factors and measurement errors. The molecular marking technology is costly and requires many experimental instruments.
Method used
A primer set was designed to identify snails, short-jawed snails and their hybrids. By agarose gel electrophoresis detection method, A or primer B was PCR amplified by primer, and the band size difference of the amplified product was identified.
Benefits of technology
This method is simple to operate, intuitive results, low cost, no advanced experimental equipment is required, and can quickly identify snails, short-jawed snails and hybrids, with high accuracy.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention provides a primer group for identifying coilia ectenes, coilia brachygnathus and hybrids thereof, a detection method, a kit and application, and belongs to the technical field of molecular marker identification. In order to overcome the defects that morphological differences of coilia ectenes, coilia brachygnathus and hybrid adults are not obvious and the like, a primer pair A or a primer pair B is designed for gene amplification, and the coilia ectenes, the coilia brachygnathus and the hybrid adults are identified by comparing sequence differences after amplification. The sequences of amplification products of the coilia ectenes, the coilia brachygnathus and the hybrids have obvious differences, the types of the amplification products can be classified through molecular markers, and the reaction result is directly visualized in the form of a gel electrophoretogram without a sequencing means. The method is simple to operate, intuitive in result, low in requirement on experimental equipment and low in cost, and all work can be completed within several hours without statistics. According to the invention, the hybrid can be identified through a sequencing peak map, and compared with an SNP site identification type, the difference is more obvious, the statistical frequency is not needed, and the accuracy is high.
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