Efficient and accurate cucumber fruit length identification method
By measuring the ovary length on the day of cucumber blossom and analyzing the correlation with the fruit length of the commercial melon period, the problem of traditional cucumber fruit length identification methods being affected by environmental factors during the commercial melon period was solved, and efficient and accurate fruit length identification was achieved, improving the efficiency of trait investigation.
Patent Information
- Application Number
- CN202411800289.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-09
- Publication Date
- 2025-03-18
AI Technical Summary
The traditional cucumber fruit length identification method was carried out during the commercial melon period. Due to factors such as weather and fertilizer and water, the fruit development rate is inconsistent, making it difficult to accurately define the commercial melon period, affecting the trait survey results.
By measuring the ovary length on the day of cucumber blossom and conducting correlation analysis with the fruit length during the commercial melon period, the measurement was performed using 6:00-8:00 in the morning during the low temperature period to reduce interference from environmental factors and improve measurement accuracy.
It realizes efficient and accurate identification of cucumber fruit length, reduces the time for trait investigation, eliminates the impact of environmental factors on fruit length, and improves the efficiency of trait investigation.
Smart Images
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Abstract
Description
Technical Field
[0001] The present application relates to the field of agricultural planting, and in particular, to a method for identifying the length of cucumber fruits. Background Art
[0002] Cucumber fruit length is an important economic trait related to yield. Under normal cultivation conditions, the female flowers of cucumbers generally open at 6 a.m. and reach the commercial fruit stage 8-10 days after flowering. In scientific research practice, the traditional phenotypic identification of cucumber fruit length is generally carried out during the commercial fruit stage (Zhao et al., 2019; Che et al., 2023). However, due to the influence of factors such as weather and fertilizer and water, the fruit development rate is not uniform, and the commercial fruit stage is difficult to define. It is easy to have inconsistent fruit development or even bent fruits, which affects the results of trait surveys. Therefore, the accuracy of traditional cucumber fruit length identification methods needs to be improved, and a more accurate and efficient fruit length measurement technology is urgently needed in molecular breeding and production practice.
[0003] Combined with the results of previous studies, the ovary length of cucumber on the day of flowering generally does not exceed 10 cm, while the length of cucumber fruit of different germplasms during the commercial melon period varies from 10 to 50 cm (Flora of China, 2019), which makes it more convenient to measure the ovary length on the day of flowering than to measure the commercial melon period. At the same time, the ovary length on the day of flowering is strongly correlated with the fruit length, and the key genetic factors that regulate fruit shape play a role in the early development of the ovary (Perin et al. 2002; Johnson and Malladi 2011; Rosati et al. 2011; Ramamurthy and Waters 2015; Colle et al., 2017; Xie et al., 2023). Therefore, measuring the ovary length on the day of flowering can not only intuitively show the important quality trait of fruit length, but also avoid more environmental factors and experimental errors caused by inaccurate measurement of commercial melon fruits, thereby improving the efficiency of the trait investigation of cucumber fruit length and the mining of candidate regulatory genes. At the same time, cucumber fruit development is a dynamic process. Measuring the ovary length on the day of flowering between 6:00-8:00 in the morning during the low temperature period can more intuitively and efficiently eliminate interference from other factors, thereby reducing measurement errors.
[0004] Application Contents
[0005] At present, the traditional phenotypic identification of cucumber fruit length is generally carried out during the commercial melon period; however, due to the influence of factors such as weather and fertilizer and water, the fruit development rate is not uniform, and the commercial melon period is difficult to define. It is easy to have inconsistent fruit development or even bent melons, which affects the results of trait investigation. In order to solve the above problems, this application adopts a method for measuring the length of cucumber fruits efficiently and accurately, which can identify the fruit length phenotype during the ovary period of the cucumber flowering day.
[0006] The efficient and accurate cucumber fruit length identification method of the present application comprises the following steps:
[0007] The ovary length was measured on the day when the cucumber female flower bloomed;
[0008] When the cucumbers reach the marketable stage, measure the length of the marketable fruit;
[0009] A correlation analysis was conducted between the ovary length and the commercial melon length on the day of flowering;
[0010]
[0011] In certain preferred embodiments, the time for measuring the ovary length of cucumber on the flowering day is the low temperature period in the morning.
[0012] In certain preferred embodiments, the time for measuring the ovary length of the cucumber on the flowering day is between 6:00 and 8:00 in the morning.
[0013] In certain preferred embodiments, the ovary length is the length from the base to the apex of the ovary on the day of flowering.
[0014] In certain preferred embodiments, the ovary length of the cucumber on the flowering day and the commercial melon length are measured using a vernier caliper and a ruler, respectively.
[0015] In certain preferred embodiments, the vernier caliper is an electronic vernier caliper.
[0016] In certain preferred embodiments, the ovary length of the cucumber on the flowering day and the length of the commercial melon are measured 4-7 times, and the average value is calculated.
[0017] In certain preferred embodiments, the number of times the ovary length of cucumbers is measured on the flowering day and the length of commercial melons is measured is 5 times.
[0018] In certain preferred embodiments, the number of times the ovary length of cucumbers is measured on the flowering day and the length of commercial melons is measured is 5 times.
[0019] In certain preferred embodiments, the invention also includes a correlation parameter between the ovary length on the day of flowering and the length of commercial melons, wherein the ovary length of cucumber on the day of flowering is significantly positively correlated with the length of commercial melons.
[0020] The correlation coefficient calculated based on the ovary length on the day of flowering and the length of commercial melons was 0.5291, which was greater than 0.4, indicating that the ovary length on the day of flowering and the length of commercial melons were significantly correlated.
[0021] Measuring the ovary length on the day of flowering can not only intuitively show the important quality trait of fruit length, but also avoid more environmental factors and experimental errors caused by inaccurate measurement of commercial melons and fruits, thereby improving the efficiency of trait investigation of cucumber fruit length and candidate regulatory gene mining. At the same time, cucumber fruit development is a dynamic process. Choosing 6:00-8:00 in the morning during the low temperature period to measure the ovary length on the day of flowering can more intuitively and efficiently eliminate interference from other factors, thereby reducing measurement errors.
[0022] Multiple measurements are taken to obtain an average value, which can reduce errors. In the present application, 4 to 7 measurements are performed, preferably 5 times.
[0023] Beneficial Effects
[0024] The present invention can realize efficient and accurate identification of cucumber fruit length. The identification method is simple, convenient and efficient, greatly reduces the trait investigation time and eliminates the influence of environmental factors on cucumber fruit length. DETAILED DESCRIPTION
[0025] The embodiments of the present application will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present application and should not be considered as limiting the scope of the present application. In the examples, if specific conditions are not specified, they are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.
[0026] The features and performance of the present application are further described in detail below in conjunction with the embodiments.
[0027] Example 1
[0028] Ten plants of the dry cucumber variety 'WLJL' with consistent growth were selected. The length of the cucumber ovaries on the day of flowering was measured using a digital 20-division vernier caliper at 6:00 in the morning. The length of the cucumber ovaries from the base to the top was recorded. Five biological replicates were recorded for each plant, and the average value was taken. The length of the cucumber ovaries on the day of flowering was then measured with a ruler.
[0029] When the cucumbers grow into commercial melons, the length of the commercial melons is measured again with a ruler. The measurement time is also recorded. The specific data is shown in Table 1.
[0030] Table 1 Comparison of different measurement methods and time for cucumber fruit length
[0031] Table 1 Comparison of different measurement methods and time for cucumber fruit length
[0032]
[0033]
[0034] The length of the cucumber ovary on the day of flowering was 2.887±0.1355cm when measured with an electronic vernier caliper; the length of the cucumber ovary on the day of flowering was 2.86±0.1284cm when measured with a ruler; and the length of the cucumber fruit during the commercial melon period was 22.13±0.8305cm when measured with a ruler. Compared with the ruler measurement method, the electronic vernier caliper can measure data accurately to two decimal places, while the ruler can only measure the last digit, so the vernier caliper has higher accuracy. At the same time, compared with the measurement method of the fruit length during the commercial melon period, the two methods of measuring the ovary length on the day of flowering have higher accuracy and smaller standard error than the fruit length during the commercial melon period, which reflects the accuracy of the electronic vernier caliper in measuring the length of cucumber fruit.
[0035] The specific time data of the two measurement methods are shown in Table 2.
[0036] Table 2 Comparison of time used by two measurement methods
[0037]
[0038] By comparing the measurement time of the three methods, it was found that the average time for the electronic vernier caliper to measure the ovary length on the day of flowering was 2.80 seconds, with a standard error of 0.1441, the average time for the ruler to measure the ovary length on the day of flowering was 3.94 seconds, with a standard error of 0.2662, and the time for the ruler to measure the length of cucumber fruit in the commercial melon period was 4.38 seconds, with a standard error of 0.1803. Compared with the method of using a ruler to measure the fruit and the ovary on the day of flowering in the commercial melon period, the time for the electronic vernier caliper to measure the ovary length on the day of flowering was 1.14 seconds and 1.58 seconds shorter than the two methods, respectively. Therefore, the measurement time of the fruit length in the commercial melon period> the measurement time of the ovary length on the day of flowering by the ruler> the measurement time of the ovary length on the day of flowering by the electronic vernier caliper. As shown in Table 2, using a vernier caliper to measure the ovary length on the day of flowering can save more than 25 minutes of measurement time, ensure that the measurement is completed quickly in a short time, avoid experimental errors caused by factors such as the growth of the plant itself, and reflect the efficiency of this method.
[0039] 20 'WLJL' cucumbers were selected, and the ovary length of each cucumber at similar nodes on the day of flowering and the length of the ovary when it grew to the commercial cucumber period were measured. Correlation analysis was performed to obtain correlation analysis data with a correlation coefficient of r=0.5291.
[0040]
[0041] Cucumber fruit or ovary length is an important yield trait with dynamic development. By measuring the ovary length of cucumber on the day of flowering at 6 am when the female flowers open and at a specific low temperature period of 6:00-8:00 am, the measurement error caused by environmental changes and fruit development is greatly reduced; by using a digital 20-division vernier caliper for length measurement, the accuracy and efficiency of the measurement results are ensured. This study provides an efficient and accurate method for cucumber fruit length identification, providing important technical support for cucumber planting and research.
[0042] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present application.
Claims
1. A method for identifying cucumber fruit length, characterized in that: The following steps are involved: The ovary length was measured on the day when the cucumber female flower bloomed; When the cucumber fruit reaches the commercial stage, measure the length of the commercial fruit; The ovary length on the flowering day and the commercial melon length were subjected to correlation analysis, and the correlation coefficient was as follows 2. The method for identifying the length of cucumber fruit according to claim 1, characterized in that: The time for measuring the ovary length of cucumber on the day of flowering is the low temperature period in the morning.
3. The method for identifying the length of cucumber fruit according to claim 2, characterized in that: The ovary length of the cucumber was measured between 6 and 8 in the morning on the day of flowering.
4. The method for identifying the length of cucumber fruit according to claim 1, characterized in that: The ovary length is the length from the base to the top of the ovary on the day of flowering.
5. The method for identifying the length of cucumber fruit according to claim 1, characterized in that: The ovary length of the cucumber on the flowering day and the length of the commercial melon were measured using a vernier caliper and a ruler, respectively.
6. The method for identifying the length of cucumber fruit according to claim 5, characterized in that: The vernier caliper is an electronic vernier caliper.
7. The method for identifying the length of cucumber fruit according to claim 5, characterized in that: The ovary length of a single cucumber plant on the day of flowering and the length of the commercial cucumber were measured 4-7 times, and the average value was calculated.
8. The method for identifying cucumber fruit length according to claim 7, characterized in that: The ovary length of a single cucumber plant on the day of flowering and the length of the commercial cucumber were measured 5 times.
9. The method for identifying the length of cucumber fruit according to claim 1, characterized in that: It also includes correlation parameters based on the ovary length on the flowering day and the commercial melon length. The cucumber fruit length can be efficiently and accurately identified based on the newly measured ovary data of the cucumber on the flowering day.