Bacteria immobilization carrier preparation device and use method thereof

Abstract

The invention discloses a bacterial immobilized carrier preparation device and a use method thereof, and relates to the technical field of immobilized bacteria, the technical scheme is that the bacterial immobilized carrier preparation device comprises a bacterial fermentation tank, a mixing tank and a carrier cross-linking bottle, and the upper parts of the bacterial fermentation tank and the mixing tank are fixedly connected with a mixing mechanism; the mixing mechanism comprises a stirring shell fixedly connected to the tops of the bacterial fermentation tank and the mixing tank, a first motor is arranged in the stirring shell, and a first gear is arranged at the output end of the first motor. The fourth gear rotates to drive the third gears connected with the two sides in a meshed mode to rotate and do circular motion along the fourth gear at the same time, then the blades arranged at the bottoms of the third gears are driven to rotate and stir, the rotating speed of the stirring shell is proper, and cell rupture caused by too high speed is avoided; and the shearing damage larger than bacterial cells in the rotating process can be effectively reduced.

Description

Technical Field

[0001] The invention relates to the technical field of immobilized bacteria, and in particular to a device for preparing a bacteria immobilized carrier and a method for using the same. Background Art

[0002] The background of the bacterial immobilized carrier preparation device stems from the development of microbial immobilization technology. As early as the beginning of the 19th century, people discovered that microbial cells have a natural tendency to adsorb on the surface of solid substances. Since the 1960s, the research on immobilized enzymes has developed rapidly. In the 1970s, the immobilization of microbial cells themselves also attracted widespread attention. Immobilization technology fixes enzymes or cells on insoluble carriers by physical or chemical methods, which can significantly improve the thermal and pH stability of enzymes, while making production continuous, saving energy and reducing costs. The bacterial immobilized carrier preparation device came into being in this context. It provides an optimized working environment for microorganisms through microbial immobilization technology, reduces strain loss, and improves strain resistance to adverse environmental factors, thereby extending the working efficiency and duration of denitrification microecological preparation products.

[0003] After searching, the invention patent of Chinese patent number CN116694456A discloses a device for preparing a bacterial immobilized carrier and a method for using the same, including a first motor, a bacterial fermentation tank, a bacterial culture reserve solution, a mixing tank, and a carrier cross-linking bottle. The bottom of the first motor is rotatably connected to a first stainless steel connecting rod, and the side of the first stainless steel connecting rod is integrally connected to a first plastic blade, and a bacterial culture medium is arranged on the outside of the first plastic blade. The bacterial fermentation tank is tightly fitted to the outside of the bacterial culture medium, and a first heating rod is installed on one side of the bacterial fermentation tank, and a first thermometer probe is installed on the other side of the bacterial fermentation tank, and a first sealing cover is tightly fitted on the top of the bacterial fermentation tank. The present invention facilitates the use of microbial immobilization technology to provide an optimized environment for efficient work of microorganisms, reduce strain loss, and improve strain resistance to adverse environmental factors, thereby extending the working efficiency and duration of the denitrification microecological preparation product. Compared with the existing technology, the invention patent with Chinese patent number CN116694456A solves the problems of insufficient organic carbon nutrition in pond aquaculture wastewater in the existing bacterial immobilization carrier preparation, low denitrification efficiency of the sprayed microorganisms due to the lack of organic carbon source in the aquaculture water, and the loss of high-efficiency strains from the aquaculture system easily caused by rainfall and water exchange processes.

[0004] However, in actual use of the above-mentioned device, the stirring mechanism arranged in the bacterial fermentation tank and the mixing tank only stirs the blades by a motor, the mixing effect is poor and it is difficult to meet the dissolved oxygen demand, resulting in difficulty for the bacteria to fully contact nutrients and oxygen, and the activity is reduced. Therefore, a device for preparing a bacterial immobilization carrier and a method for using the same are proposed. Summary of the invention

[0005] The purpose of the present invention is to solve the shortcomings of the prior art that the stirring mechanism arranged in the bacterial fermentation tank and the mixing tank only stirs the blades by a motor, the mixing effect is poor and it is difficult to meet the dissolved oxygen demand, resulting in difficulty for the bacteria to fully contact nutrients and oxygen and reduced activity, and a bacterial immobilization carrier preparation device and a method of using the same are proposed.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A bacteria immobilization carrier preparation device and a use method thereof, comprising a bacteria fermentation tank, a mixing tank and a carrier cross-linking bottle, wherein the bacteria fermentation tank and the mixing tank are fixedly connected with a mixing mechanism at the top, the mixing mechanism comprises a stirring shell fixedly connected to the top of the bacteria fermentation tank and the mixing tank, a first motor is arranged inside the stirring shell, a first gear is arranged at the output end of the first motor, the first gear is meshedly connected with a second gear, a fourth gear is fixedly connected to the bottom of the second gear, the fourth gear is rotatably connected to the stirring shell, third gears are meshedly connected to the fourth gear on both sides, a rack is meshedly connected to the third gear away from the fourth gear, the rack is fixedly connected to the stirring shell, a rotating shaft is fixedly connected to the bottom of the third gear, a paddle is arranged at the bottom of the rotating shaft, the fourth gear is driven to rotate by the first motor, the rotation of the fourth gear drives the third gear meshedly connected on both sides to rotate while making a circular motion along the fourth gear, thereby driving the paddle arranged at the bottom to stir;

[0008] A telescopic shell is fixedly connected to the bottom of the fourth gear, a second motor is arranged inside the telescopic shell, an eccentric shaft is arranged at the output end of the second motor, the eccentric shaft is rotatably connected to a connecting rod, a rotating shaft is fixedly connected to the bottom of the connecting rod, and the eccentric shaft is driven to rotate by the second motor, so that the rotating shaft reciprocates up and down, driving the paddles to move up and down, the bacterial fermentation tank, the mixing tank and the carrier cross-linking bottle are connected by a peristaltic pump, and a magnetic stirrer is arranged at the bottom of the carrier cross-linking bottle.

[0009] The above technical solution further includes:

[0010] A long rubber tube is fixedly connected to the left side of the peristaltic pump, and a short rubber tube is fixedly connected to the right side of the peristaltic pump. The long rubber tube can be inserted into the bottom of the bacterial fermentation tank and the mixing tank, and the short rubber tube is located at the upper part of the bacterial fermentation tank and the mixing tank away from the telescopic shell, and will not contact the solution in the bacterial fermentation tank and the mixing tank;

[0011] The bacterial fermentation tank and the mixing tank are both provided with exhaust pipes at the top, and a sterile filter membrane is provided inside the exhaust pipes.

[0012] A feed pipe is fixedly connected to the upper part of the mixing tank, and a sealing cover is threadedly connected to the top of the feed pipe.

[0013] The bacterial fermentation tank and the mixing tank are both provided with temperature control mechanisms, and the temperature control mechanisms display and control the internal temperatures of the bacterial fermentation tank and the mixing tank.

[0014] The temperature control mechanism includes a temperature controller fixedly connected to the front end of the bacterial fermentation tank and the mixing tank, a heating rod and a temperature sensor are arranged at the rear end of the temperature controller, the heating rod and the temperature sensor are located inside the bacterial fermentation tank and the mixing tank and are in direct contact with the solution, a display screen is arranged on the upper part of the temperature controller, and the temperature inside the bacterial fermentation tank and the mixing tank can be displayed through the display screen, and a control button is arranged at the lower part of the temperature controller, and the temperature inside the bacterial fermentation tank and the mixing tank can be adjusted through the control button.

[0015] An ultraviolet lamp is arranged in the middle of the bacterial fermentation tank and the mixing tank, and the ultraviolet lamp irradiates the inside of the bacterial fermentation tank and the mixing tank.

[0016] An exhaust pipe is fixedly connected to the upper part of the carrier cross-linking bottle, an addition pipe is arranged on one side of the exhaust pipe, one end of the addition pipe is located inside the carrier cross-linking bottle and is inserted into the bottom of the carrier cross-linking bottle, and an electric valve is arranged on the upper part of the addition pipe.

[0017] A heating plate is arranged on the top of the magnetic stirrer, a carrier cross-linking bottle is placed on the upper part of the heating plate, and an adjusting button is arranged at the front end of the magnetic stirrer.

[0018] A method for using a bacteria immobilization carrier preparation device comprises the following steps:

[0019] Step 1: Screening out strains suitable for immobilization from the strain library, culturing the screened strains on a culture medium containing appropriate nutrients, and obtaining the growth state and growth characteristics of the strains;

[0020] Step 2: inoculating the cultured strain into a bacterial fermentation tank for fermentation. During the fermentation process, the mixing mechanism is started to drive the paddles to rotate, stirring the bacterial fermentation tank, and waiting for the bacteria in the bacterial fermentation tank to grow;

[0021] Step 3: Add an appropriate amount of embedding agent and nutrient solution into the mixing tank, transport the fermented bacterial solution into the mixing tank through a peristaltic pump, start the stirring mechanism provided on the mixing tank, and drive the paddles to rotate through the stirring mechanism to mix the embedding agent and nutrient solution evenly;

[0022] Step 4: The mixed liquid is transported to the carrier cross-linking bottle through a peristaltic pump, and an appropriate amount of cross-linking agent is added to the carrier cross-linking bottle to mix with the mixed liquid. The solution in the carrier cross-linking bottle is stirred by a magnetic stirrer to ensure that the carrier beads are evenly generated. Under appropriate conditions, the carrier beads are gradually solidified under the action of the cross-linking agent.

[0023] The present invention has the following beneficial effects:

[0024] 1. In the present invention, during the fermentation of bacteria in the bacterial fermentation tank and the mixing process in the mixing tank, the first motor inside the stirring shell can be started to drive the fourth gear to rotate. The rotation of the fourth gear drives the third gear meshing on both sides to rotate while making a circular motion along the fourth gear, thereby driving the paddles arranged at the bottom of the third gear to rotate and stir. The rotation speed of the stirring shell is appropriate and will not cause cell rupture due to excessive speed. The paddles are paddle-type structures, which can effectively reduce shear damage greater than bacterial cells during the rotation process. By driving the paddles to rotate and revolve at the same time, the mixing effect of the equipment can be effectively improved, and the preparation efficiency can be improved. The second motor can also drive the paddles to move back and forth up and down. In this way, the dissolved oxygen demand of the bacteria is effectively met and the bacterial activity is improved.

[0025] 2. In the present invention, an ultraviolet lamp is arranged in the middle position of the bacterial fermentation tank and the mixing tank, and the prepared liquid can be sterilized by the ultraviolet lamp to reduce the risk of contamination. An exhaust pipe is arranged on the upper part of the bacterial fermentation tank and the mixing tank, and the gas inside the bacterial fermentation tank and the mixing tank can be discharged through the exhaust pipe. In addition, a sterile filter membrane is arranged inside the exhaust pipe to prevent external bacteria from contaminating the prepared liquid, thereby ensuring a sterile environment for bacterial immobilization. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic structural diagram of a bacteria immobilization carrier preparation device and a method for using the same proposed by the present invention;

[0027] Figure 2 This is a schematic diagram of the internal structure of the bacterial fermentation tank in the present invention;

[0028] Figure 3 It is a schematic diagram of the structure of the temperature control mechanism in the present invention;

[0029] Figure 4 This is a schematic diagram of the internal structure of the mixing tank in the present invention;

[0030] Figure 5 It is a schematic diagram of the stirring mechanism structure in the present invention;

[0031] Figure 6 It is a schematic diagram of the internal structure of the stirring shell in the present invention;

[0032] Figure 7It is a schematic diagram of the internal structure of the telescopic shell in the present invention.

[0033] In the figure: 1. bacterial fermentation tank; 2. mixing tank; 3. carrier cross-linking bottle; 4. magnetic stirrer; 5. heating plate; 6. adjusting knob; 7. ultraviolet lamp; 8. temperature controller; 9. peristaltic pump; 10. stirring shell; 11. exhaust pipe; 12. long rubber tube; 13. short rubber tube; 14. heating rod; 15. temperature sensor; 16. electric valve; 17. rotating shaft; 18. paddle; 19. telescopic shell; 20. first motor; 21. first gear; 22. second gear; 23. third gear; 24. fourth gear; 25. rack; 26. second motor; 27. eccentric shaft; 28. connecting rod; 29. ​​adding tube; 30. feeding tube. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Embodiment 1

[0036] like Figure 1-Figure 7 As shown, a bacterial immobilization carrier preparation device and a method of using the same proposed by the present invention include a bacterial fermentation tank 1, a mixing tank 2 and a carrier cross-linking bottle 3, the bacterial fermentation tank 1 and the mixing tank 2 are fixedly connected with a mixing mechanism on the top, the mixing mechanism includes a stirring shell 10 fixedly connected to the top of the bacterial fermentation tank 1 and the mixing tank 2, a first motor 20 is arranged inside the stirring shell 10, a first gear 21 is arranged at the output end of the first motor 20, the first gear 21 is meshedly connected with a second gear 22, the bottom of the second gear 22 is fixedly connected with a fourth gear 24, and the fourth gear 24 is connected to the The stirring shell 10 is rotatably connected, the third gear 23 is meshedly connected on both sides of the fourth gear 24, the third gear 23 is meshedly connected with a rack 25 on the side away from the fourth gear 24, the rack 25 is fixedly connected to the stirring shell 10, the bottom of the third gear 23 is fixedly connected to a rotating shaft 17, and a paddle 18 is arranged at the lower part of the rotating shaft 17, and the fourth gear 24 is driven to rotate by the first motor 20. The rotation of the fourth gear 24 drives the third gear 23 meshed on both sides to rotate while making a circular motion along the fourth gear 24, thereby driving the paddle 18 arranged at the bottom to stir.

[0037] A telescopic shell 19 is fixedly connected to the bottom of the fourth gear 24, and a second motor 26 is arranged inside the telescopic shell 19. An eccentric shaft 27 is arranged at the output end of the second motor 26. The eccentric shaft 27 is rotatably connected to a connecting rod 28. The bottom of the connecting rod 28 is fixedly connected to a rotating shaft 17. The eccentric shaft 27 is driven to rotate by the second motor 26, so that the rotating shaft 17 reciprocates up and down, driving the paddle 18 to move up and down. The bacterial fermentation tank 1, the mixing tank 2 and the carrier cross-linking bottle 3 are connected by a peristaltic pump 9, and a magnetic stirrer 4 is arranged at the bottom of the carrier cross-linking bottle 3.

[0038] A long rubber tube 12 is fixedly connected to the left side of the peristaltic pump 9, and a short rubber tube 13 is fixedly connected to the right side of the peristaltic pump 9. The long rubber tube 12 can be inserted into the bottom of the bacterial fermentation tank 1 and the mixing tank 2, and the short rubber tube 13 is located at the upper part of the bacterial fermentation tank 1 and the mixing tank 2 at one end away from the telescopic shell 19, and will not contact the solution in the bacterial fermentation tank 1 and the mixing tank 2.

[0039] In the present embodiment, when in use, firstly, strains suitable for immobilization are screened out from the strain library, and the screened strains are cultured on a culture medium containing appropriate nutrients, and then the cultured strains are inoculated into the bacterial fermentation tank 1 for fermentation and culture. During the fermentation process, the first motor 20 inside the stirring shell 10 is started, and the first motor 20 drives the first gear 21 to rotate, and the rotation of the first gear 21 drives the meshing second gear 22 to rotate, and the rotation of the second gear 22 can drive the fixedly connected fourth gear 24 to rotate, and the third gear 23 meshing on both sides can be driven to rotate by the fourth gear 24 while performing a circular motion along the fourth gear 24, and then the paddle 18 arranged at the bottom of the third gear 23 can be driven to rotate and stir, and the rotation speed of the stirring shell 10 during stirring is appropriate and will not cause cell rupture due to excessive speed, and the paddle 18 is a paddle-type structure, which can effectively reduce the shear damage greater than the bacterial cells during the rotation process, and by driving the paddle 18 to rotate while revolving, the mixing effect of the equipment can be effectively improved, and the preparation efficiency can be improved.

[0040] A telescopic shell 19 is fixedly connected to the bottom of the fourth gear 24. By starting the second motor 26 inside the telescopic shell 19, the eccentric shaft 27 can be driven to rotate. The rotation of the eccentric shaft 27 drives the connecting rod 28 to rotate. The rotation of the connecting rod 28 can drive the rotating shaft 17 to move up and down, thereby driving the paddle 18 to move back and forth up and down. This stirring method can effectively meet the dissolved oxygen demand of bacteria and improve bacterial activity. After the bacteria are fermented, the fermented bacterial liquid is transported to the mixing tank 2 through the peristaltic pump 9, and the stirring mechanism provided on the mixing tank 2 is started to mix the embedding agent and the nutrient solution evenly. Finally, the mixed liquid is transported to the carrier cross-linking bottle 3 through the peristaltic pump 9, and an appropriate amount of cross-linking agent is added to the carrier cross-linking bottle 3 to mix with the mixed liquid, so that the carrier balls are gradually solidified under the action of the cross-linking agent.

[0041] Embodiment 2

[0042] like Figure 1-Figure 7 As shown, based on the first embodiment, an exhaust pipe 11 is provided on the top of the bacterial fermentation tank 1 and the mixing tank 2, a sterile filter membrane is provided inside the exhaust pipe 11, a feed pipe 30 is fixedly connected to the top of the mixing tank 2, a sealing cover is threadedly connected to the top of the feed pipe 30, and a temperature control mechanism is provided inside the bacterial fermentation tank 1 and the mixing tank 2, which displays and controls the internal temperature of the bacterial fermentation tank 1 and the mixing tank 2.

[0043] The temperature control mechanism includes a temperature controller 8 fixedly connected to the front end of the bacterial fermentation tank 1 and the mixing tank 2, a heating rod 14 and a temperature sensor 15 are arranged at the rear end of the temperature controller 8, the heating rod 14 and the temperature sensor 15 are located inside the bacterial fermentation tank 1 and the mixing tank 2 and are in direct contact with the solution, a display screen is arranged on the upper part of the temperature controller 8, and the temperature inside the bacterial fermentation tank 1 and the mixing tank 2 can be displayed through the display screen, a control button is arranged at the lower part of the temperature controller 8, and the temperature inside the bacterial fermentation tank 1 and the mixing tank 2 can be adjusted through the control button, and an ultraviolet lamp 7 is arranged in the middle position of the bacterial fermentation tank 1 and the mixing tank 2, and the ultraviolet lamp 7 irradiates the inside of the bacterial fermentation tank 1 and the mixing tank 2.

[0044] An exhaust pipe 11 is fixedly connected to the top of the carrier cross-linking bottle 3, and an addition pipe 29 is arranged on one side of the exhaust pipe 11. The addition pipe 29 is located inside the carrier cross-linking bottle 3 and one end is inserted into the bottom of the carrier cross-linking bottle 3. An electric valve 16 is arranged on the top of the addition pipe 29, and a heating plate 5 is arranged on the top of the magnetic stirrer 4. The carrier cross-linking bottle 3 is placed on the top of the heating plate 5. An adjusting button 6 is arranged at the front end of the magnetic stirrer 4.

[0045] In this embodiment, during the fermentation and mixing process of bacteria in the bacterial fermentation tank 1 and the mixing tank 2, the temperature inside the bacterial fermentation tank 1 and the mixing tank 2 can be detected by the temperature sensor 15 arranged on the temperature controller 8, and the detected data can be displayed on the display screen on the temperature controller 8, and the heating rod 14 can also be controlled by the operating button on the temperature controller 8 to heat the inside of the bacterial fermentation tank 1 and the mixing tank 2, so as to ensure that the bacteria grow and immobilize at a suitable temperature. An ultraviolet lamp 7 is arranged in the middle position of the bacterial fermentation tank 1 and the mixing tank 2, and the prepared liquid can be sterilized by the ultraviolet lamp 7 to reduce the risk of contamination. An exhaust pipe 11 is arranged on the upper part of the bacterial fermentation tank 1 and the mixing tank 2, and the gas inside the bacterial fermentation tank 1 and the mixing tank 2 can be discharged through the exhaust pipe 11. In addition, a sterile filter membrane is arranged inside the exhaust pipe 11 to prevent external bacteria from contaminating the prepared liquid and ensure a sterile environment for bacterial immobilization. Finally, when the bacteria are solidified in the carrier cross-linking bottle 3, the reaction temperature can be adjusted by adjusting the heating plate 5 by adjusting the adjustment button 6, thereby improving the use effect of the device.

[0046] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A bacterial immobilization carrier preparation device, comprising a bacterial fermentation tank (1), a mixing tank (2) and a carrier cross-linking bottle (3), characterized in that: A mixing mechanism is fixedly connected to the upper part of the bacterial fermentation tank (1) and the mixing tank (2), and the mixing mechanism comprises a stirring shell (10) fixedly connected to the top of the bacterial fermentation tank (1) and the mixing tank (2), a first motor (20) is arranged inside the stirring shell (10), a first gear (21) is arranged at the output end of the first motor (20), the first gear (21) is meshedly connected to a second gear (22), a fourth gear (24) is fixedly connected to the bottom of the second gear (22), the fourth gear (24) is rotatably connected to the stirring shell (10), and the fourth gear (24) is rotated at both ends. A third gear (23) is meshedly connected to the side of the third gear (23), and a rack (25) is meshedly connected to the side of the third gear (23) away from the fourth gear (24). The rack (25) is fixedly connected to the stirring shell (10). A rotating shaft (17) is fixedly connected to the bottom of the third gear (23). A paddle (18) is arranged at the lower part of the rotating shaft (17). The fourth gear (24) is driven to rotate by the first motor (20). The rotation of the fourth gear (24) drives the third gears (23) meshedly connected on both sides to rotate while making a circular motion along the fourth gear (24), thereby driving the paddle (18) arranged at the bottom to stir; The bottom of the fourth gear (24) is fixedly connected to a telescopic housing (19), a second motor (26) is arranged inside the telescopic housing (19), an eccentric shaft (27) is arranged at the output end of the second motor (26), the eccentric shaft (27) is rotatably connected to a connecting rod (28), the bottom of the connecting rod (28) is fixedly connected to a rotating shaft (17), the eccentric shaft (27) is driven to rotate by the second motor (26), so that the rotating shaft (17) reciprocates up and down, driving the paddle (18) to move up and down, the bacterial fermentation tank (1), the mixing tank (2) and the carrier cross-linking bottle (3) are connected by a peristaltic pump (9), and a magnetic stirrer (4) is arranged at the bottom of the carrier cross-linking bottle (3).

2. The device for preparing a bacteria immobilized carrier according to claim 1, characterized in that: A long rubber tube (12) is fixedly connected to the left side of the peristaltic pump (9), and a short rubber tube (13) is fixedly connected to the right side of the peristaltic pump (9).

3. The device for preparing a bacteria immobilized carrier according to claim 1, characterized in that: An exhaust pipe (11) is arranged on the top of the bacterial fermentation tank (1) and the mixing tank (2), and a sterile filter membrane is arranged inside the exhaust pipe (11).

4. The device for preparing a bacteria immobilized carrier according to claim 1, characterized in that: A feed pipe (30) is fixedly connected to the upper portion of the mixing tank (2), and a sealing cover is threadedly connected to the top of the feed pipe (30).

5. The device for preparing a bacteria immobilized carrier according to claim 1, characterized in that: The bacterial fermentation tank (1) and the mixing tank (2) are both provided with temperature control mechanisms, and the temperature control mechanisms display and control the internal temperatures of the bacterial fermentation tank (1) and the mixing tank (2).

6. The device for preparing a bacteria immobilized carrier according to claim 5, characterized in that: The temperature control mechanism comprises a temperature controller (8) fixedly connected to the front end of the bacterial fermentation tank (1) and the mixing tank (2); a heating rod (14) and a temperature sensor (15) are arranged at the rear end of the temperature controller (8); the heating rod (14) and the temperature sensor (15) are located inside the bacterial fermentation tank (1) and the mixing tank (2) and are in direct contact with the solution.

7. The device for preparing a bacteria immobilized carrier according to claim 1, characterized in that: An ultraviolet lamp (7) is arranged in the middle of the bacterial fermentation tank (1) and the mixing tank (2), and the ultraviolet lamp (7) irradiates the inside of the bacterial fermentation tank (1) and the mixing tank (2).

8. The device for preparing a bacteria immobilized carrier according to claim 1, characterized in that: The upper part of the carrier cross-linking bottle (3) is fixedly connected to an exhaust pipe (11), one side of the exhaust pipe (11) is provided with an addition pipe (29), one end of the addition pipe (29) is located inside the carrier cross-linking bottle (3) and is inserted into the bottom of the carrier cross-linking bottle (3), and the upper part of the addition pipe (29) is provided with an electric valve (16).

9. The device for preparing a bacteria immobilized carrier according to claim 1, characterized in that: A heating plate (5) is arranged on the top of the magnetic stirrer (4), a carrier cross-linking bottle (3) is placed on the top of the heating plate (5), and an adjusting button (6) is arranged at the front end of the magnetic stirrer (4).

10. The method for using the device for preparing a bacteria immobilized carrier according to claim 1, characterized in that: The following steps are involved: Step 1: Screening out strains suitable for immobilization from the strain library, culturing the screened strains on a culture medium containing appropriate nutrients, and obtaining the growth state and growth characteristics of the strains; Step 2: inoculating the cultured strain into a bacterial fermentation tank (1) for fermentation. During the fermentation process, the mixing mechanism is started to drive the paddle (18) to rotate, thereby stirring the bacterial fermentation tank (1), and waiting for the bacteria in the bacterial fermentation tank (1) to grow; Step 3: Add appropriate amounts of embedding agent and nutrient solution to the mixing tank (2), transport the fermented bacterial solution to the mixing tank (2) through a peristaltic pump (9), start a stirring mechanism provided on the mixing tank (2), and drive the paddle (18) to rotate through the stirring mechanism to mix the embedding agent and nutrient solution evenly; Step 4: The mixed liquid is transported to the carrier cross-linking bottle (3) through a peristaltic pump (9), an appropriate amount of a cross-linking agent is added to the carrier cross-linking bottle (3) and mixed with the mixed liquid, the solution in the carrier cross-linking bottle (3) is stirred by a magnetic stirrer (4) to ensure that the carrier beads are uniformly generated, and under appropriate conditions, the carrier beads are gradually solidified under the action of the cross-linking agent.

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