Multi-component aggregation breeding method for peony seeds

Abstract

The invention discloses a multi-element aggregation breeding method for Chinese herbaceous peony seeds. The method comprises the following steps: establishing a multi-element Chinese herbaceous peony seed resource library; carrying out regional classification on the seed resources; carrying out germplasm integration and improvement on Chinese herbaceous peony from different geographical sources, and constructing a Chinese herbaceous peony germplasm bank with excellent root characters; determining the content of characteristic components of the bred paeonia lactiflora roots with excellent root characters, and screening paeonia lactiflora plants with high content of characteristic components; and planting and identifying in multiple places and multiple points, and selecting a peony variety which is not easy to degrade. According to the multi-element aggregation breeding method for the paeonia lactiflora seeds, paeonia lactiflora from different geographical sources is hybridized, paeonia lactiflora varieties with excellent offspring root characters and high characteristic component content are bred, varieties with stable characters are selected through multi-generation breeding, pilot tests are conducted in different regions, and the breeding quality of the paeonia lactiflora seeds is improved. Chinese herbaceous peony plants which are not easy to degrade are selected in a division propagation mode; a peony plant which is excellent in root character and not prone to degeneration is found through a multi-component optimization breeding mode, and therefore the economic value of the radix paeoniae alba is increased.

Description

Technical Field

[0001] The invention relates to the field of breeding, and in particular to a multi-element polymorphism breeding method for peony seeds. Background Art

[0002] Peony (scientific name: Paeonia lactiflora Pall.) is a perennial herbaceous plant of the genus Paeonia in the family Paeoniaceae, also known as wild peony, white peony, and peony flower. The roots are thick and the branches are dark brown; the stems are hairless, and the leaflets are narrowly ovate, elliptical or lanceolate; the flowers are borne at the top of the stems and in the axils of the leaves; the flowering period is May-June; the fruiting period is August.

[0003] The root of peony can be used as medicine, called "white peony", which can relieve pain, relieve spasms, remove blood stasis, and regulate menstruation; it can also nourish blood and regulate menstruation, restrain yin and stop sweating, soften the liver and relieve pain, and suppress liver yang. It is used to treat blood deficiency and chlorosis, irregular menstruation, spontaneous sweating, night sweats, rib pain, abdominal pain, cramps in the limbs, headaches and dizziness.

[0004] Artificially cultivated peony is mainly produced in Bozhou, Anhui (Bobaishao), Hangzhou, Zhejiang (Hangbaishao), Mianyang, Sichuan (Chuanbaishao), Hunan (Xiangbaishao), Heze, Shandong (Hezebaishao), etc. Each production area has a long history of cultivation, forming corresponding varieties. White peony is best when it is thick, firm, heavy, off-white in cross section, and has no hollows or cracks.

[0005] The root of white peony root is cylindrical, straight, with both ends flat and not concave, the head and tail are similar in size, 7~20cm long, 0.5~3cm in diameter. The outer skin is slightly rough, light brown-red, with longitudinal wrinkles and root marks. It is solid, heavy, and not easy to break. The cross section is off-white, slightly flat and slightly granular, with chrysanthemum heart texture in the middle. It has no odor and tastes slightly bitter and sour.

[0006] The root of Bobaishao is cylindrical, straight or curved, 4~15cm long, 0.8~2cm in diameter. The surface is light red or off-white, and turns brownish red after a long storage time. The skin is fine, and the longitudinal lines and root marks are not obvious. The outer skin is not cleaned and has brown spots. One end is slightly thicker, and the two ends are cut flat. The cut surface is slightly concave, and there are light red spots occasionally. The texture is solid and heavy, slightly lighter than Hangbaishao. The cross section shows a slight chrysanthemum heart texture, with cracks or skin and flesh separation. The smell is slight and the taste is slightly bitter and sour.

[0007] Sichuan white peony root includes Jiangzhong white peony root and Quhe white peony root. The root of Jiangzhong white peony root is cylindrical, slightly curved, 4~15cm long, and 0.5~1.5cm in diameter. It is thick at the head and thin at the tail, with smooth appearance, delicate skin texture, white with red, and brown concave root marks. The cross section is light pink, delicate and moist, with chrysanthemum heart texture. The smell is slight, and the taste is slightly bitter and sour. The root of Quhe white peony root is similar in size to that of Zhongjiang white peony root, but the surface is light brown, slightly rough, slightly wrinkled, and the two ends are flush, with depressions in between, and slightly straight. The cross section is off-white or light brown, with chrysanthemum heart texture. The smell is slight, and the taste is slightly bitter and sour.

[0008] The root of Hunan white peony is not straight, but slightly curved, similar in length and size to Sichuan white peony, tapering at both ends, slightly thicker in the middle, light brown in color, with rough longitudinal wrinkles and traces of fibrous roots. It is hard, off-white in cross section, with obvious chrysanthemum heart texture. It has a faint smell and tastes slightly bitter and sour.

[0009] In addition to the influence of production processes such as sun-drying, the grading of white peony is also due to the differences in the properties of the peony itself in different regions, such as the thickness of the head and tail, the degree of curvature, whether it has a young tail, powderiness, smoothness of the epidermis, and vascular bundle differences.

[0010] In addition, white peony plants only bud and bloom after 2 or 3 years old, and the fruiting rate of 3-year-old plants is low, and the seeds are not full. Traditional farm varieties are mainly propagated by division, but long-term asexual reproduction by division will cause variety degeneration, poor stress resistance, and serious decline in quality and yield.

[0011] Therefore, we seek a diversified breeding method, conduct research on the excellent traits of white peony in various regions, and look for a peony plant with excellent root traits that is not easy to degenerate, so as to increase the economic value of white peony. Summary of the invention

[0012] The purpose of the present invention is to provide a multi-element breeding method for peony seeds to solve the problems in the background technology.

[0013] To achieve the above object, the present invention provides a multivariate polymorphism breeding method for peony seeds, comprising the following steps: (1) Establish a multi-source peony seed resource bank; (2) Classify seed resources by region; (3) Integrate and improve the germplasm of peonies from different geographical origins and build a germplasm bank of peonies with excellent root traits; (4) measuring the content of characteristic components in the medicinal roots of the peony varieties with excellent root traits selected in step (3), and screening peony plants with high content of characteristic components; (5) Plant and identify peony varieties at multiple locations and select those that are less likely to degenerate; (6) Variety approval; (7) Promotion and application.

[0014] Preferably, step (1) comprises: integrating medicinal peony varieties from various regions to establish a multi-variety peony seed resource library, wherein the multi-variety peony seed resource library contains seeds with all excellent traits.

[0015] Preferably, step (2) comprises: classifying the seeds in the established multivariate peony seed resource library according to their geographical origins and excellent traits, including white peony from Bozhou, Anhui, white peony from Hangzhou, Zhejiang, white peony from Zhongjiang, Sichuan, white peony from Quhe, Sichuan, and white peony from Hunan.

[0016] Preferably, step (3) comprises: cultivating peony seeds from different geographical origins, hybridizing with peony seeds from different geographical origins as male or female parents, cultivating the first generation, and selecting varieties with excellent root traits; Hybridize the excellent varieties of the first generation with each other, or with the male or female parents with excellent traits, and continue to select varieties with excellent root traits until the traits of the offspring are stable.

[0017] Preferably, the good root traits refer to: the root is uniform in thickness, straight, with a diameter greater than or equal to 1.8 mm, without a small tail, and smooth.

[0018] Preferably, in step (4), the characteristic components include: Monoterpene glycosides: PF, AF, oxidized paeoniflorin, benzoylpeoniflorin, galloylpeoniflorin; Gallotannins: pentagalloylglucose, gallic acid; Flavonoids: catechins.

[0019] Preferably, in step (4), the characteristic components are high in: water-soluble extract ≥ 24%, PF ≥ 2.0%, and PF / AF ≥ 6.0.

[0020] Preferably, step (5) comprises: planting the peony plants selected through steps (3) and (4) at multiple test sites in several locations, using division propagation, identifying the root traits and characteristic component contents of the division plants, and selecting peony varieties that are not prone to degeneration.

[0021] An application of the above-mentioned multi-element breeding method for peony seeds in peony seed selection and breeding.

[0022] Therefore, the multivariate polymorphism breeding method of peony seeds of the present invention has the following beneficial effects: (1) Hybridize peonies from different geographical sources to select peony varieties with excellent root traits and characteristic component content in the offspring. After multiple generations of breeding, select varieties with stable traits for pilot tests in different regions, and use division propagation to further select peony plants that are less likely to degenerate; (2) Through a multi-dimensional breeding approach, we can find a peony plant with excellent root traits that is not easily degraded, thereby increasing the economic value of white peony.

[0023] The technical solution of the present invention is further described in detail below through embodiments. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical scheme and advantages disclosed in the embodiments of the present invention clearer, the embodiments of the present invention are further described in detail in conjunction with the embodiments below. It should be understood that the specific embodiments described herein are only used to explain the embodiments of the present invention and are not intended to limit the embodiments of the present invention. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the scope of protection of this application.

[0025] It should be noted that the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or server that includes a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units that are not explicitly listed or inherent to these processes, methods, products or devices.

[0026] Example A multi-element breeding method for peony seeds comprises the following steps: (1) Establish a diversified seed resource library; Comprehensively integrate the medicinal peony varieties in various regions to establish a multi-dimensional peony seed resource bank, which contains seeds with all excellent traits; (2) Classify seed resources by region; The seeds in the established multi-dimensional peony seed resource bank are classified according to their geographical origins and excellent traits, including: Bo white peony from Bozhou, Anhui, Hang white peony from Hangzhou, Zhejiang, Jiangzhong white peony from Zhongjiang, Sichuan, Quhe white peony from Quhe, Sichuan, and Xiang white peony from Hunan.

[0027] The root of Paeonia lactiflora is cylindrical, straight or curved, 4 to 15 cm long, and 0.8 to 2 cm in diameter. The root of white peony root is cylindrical and straight, with both ends flush and not concave. The head and tail are similar in size, 7~20cm long and 0.5~3cm in diameter.

[0028] The root of Jiangzhong White Peony Root is cylindrical, slightly curved, 4-15cm long, 0.5-1.5cm in diameter, thick at the head and thin at the tail, and smooth on the surface.

[0029] The roots of Quhe white peony are similar in size to those of Jiangzhong white peony, but the roots are slightly rougher.

[0030] The roots of Hunan white peony are not straight, but slightly curved. Their length and size are similar to those of Jiangzhong white peony. They gradually become thinner at both ends and slightly thicker in the middle. The surface is light brown with rough longitudinal wrinkles and remnants of root fibers.

[0031] (3) Integrate and improve the germplasm of peonies from different geographical origins and build a germplasm bank of peonies with excellent root traits; Peony seeds from different geographical origins are cultivated, hybridized with peonies from different geographical origins as male or female parents, the first generation is cultivated, and varieties with excellent root traits are selected (roots are uniform in thickness, straight, with a diameter greater than ≥1.8mm, without young tails, and smooth).

[0032] The excellent varieties of the first generation are hybridized with each other, or with the male or female parents with excellent traits, and varieties with excellent traits are continuously selected until the traits of the offspring are stable.

[0033] (4) The characteristic component contents of the medicinal roots of the peony varieties with excellent root traits selected in step (3) are measured, including the contents of eight indicator components: monoterpene glycosides [PF, AF, oxidized paeoniflorin (OPF), benzoylpeonidin (BPF), galloylpeonidin (GPF)], gallotannins [pentagalloylglucose (PGG), gallic acid (GA)] and flavonoids [catechins (CA)].

[0034] The HPLC method was used to determine the content of characteristic components, and peony plants with water-soluble extract ≥24%, PF ≥2.0%, and PF / AF ≥6.0 were screened.

[0035] (5) Identification of planting at multiple locations and points: Planting the peony plants selected through step (3) and step (4) at multiple test sites in several locations, using division propagation, identifying the root traits and characteristic component contents of the division plants, and selecting peony varieties that are not prone to degeneration; (6) Variety evaluation: Peony seeds with excellent root traits and consistent content of characteristic components will be evaluated for variety.

[0036] (7) Promotion and application: Promote and apply peony seeds with excellent root traits and satisfactory content of characteristic components.

[0037] Therefore, the present invention provides a multi-element and optimal breeding method for peony seeds, which hybridizes peonies from different geographical origins, selects peony varieties with excellent root traits and characteristic component content in offspring, and after multiple generations of breeding, selects varieties with stable traits for pilot tests in different regions, and further selects peony plants that are not easy to degenerate by using the ramet propagation method; through the multi-element and optimal breeding method, a peony plant with excellent root traits that is not easy to degenerate is found, thereby increasing the economic value of white peony.

[0038] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A multi-element polymorphism breeding method for peony seeds, characterized in that: The steps include: (1) Establish a multi-source peony seed resource bank; (2) Classify seed resources by region; (3) Integrate and improve the germplasm of peonies from different geographical origins and build a germplasm bank of peonies with excellent root traits; (4) measuring the content of characteristic components in the medicinal roots of the peony varieties with excellent root traits selected in step (3), and screening peony plants with high content of characteristic components; (5) Plant and identify peony varieties at multiple locations and select those that are less likely to degenerate; (6) Variety approval; (7) Promotion and application.

2. The multivariate polymorphism breeding method of peony seeds according to claim 1, characterized in that: Step (1) includes: integrating medicinal peony varieties from various regions to establish a multi-dimensional peony seed resource library, which contains seeds with all excellent traits.

3. The multivariate polymorphism breeding method of peony seeds according to claim 1, characterized in that: Step (2) includes: classifying the seeds in the established multi-element peony seed resource library according to their geographical origins and excellent traits, including white peony from Bozhou, Anhui, white peony from Hangzhou, Zhejiang, white peony from Zhongjiang, Sichuan, white peony from Quhe, Sichuan, and white peony from Hunan.

4. The multivariate polymorphism breeding method of peony seeds according to claim 1, characterized in that: Step (3) comprises: cultivating peony seeds from different geographical sources, hybridizing the peony seeds from different geographical sources as male or female parents, cultivating the first generation, and selecting varieties with excellent root traits; Hybridize the excellent varieties of the first generation with each other, or with the male or female parents with excellent traits, and continue to select varieties with excellent root traits until the traits of the offspring are stable.

5. A multi-element breeding method for peony seeds according to claim 1 or 4, characterized in that: Good root traits refer to: the roots are uniform in thickness, straight, ≥1.8mm in diameter, without tails, and smooth.

6. The multivariate polymorphism breeding method of peony seeds according to claim 1, characterized in that: In step (4), the characteristic components include: Monoterpene glycosides: PF, AF, oxidized paeoniflorin, benzoylpeoniflorin, galloylpeoniflorin; Gallotannins: pentagalloylglucose, gallic acid; Flavonoids: catechins.

7. The multivariate polymorphism breeding method of peony seeds according to claim 1, characterized in that: In step (4), the characteristic component high refers to: water-soluble extract ≥ 24%, PF ≥ 2.0%, PF / AF ≥ 6.

0.

8. The multivariate polymorphism breeding method of peony seeds according to claim 1, characterized in that: Step (5) includes: planting the peony plants selected through steps (3) and (4) at multiple test sites in several locations, using division propagation, identifying the root traits and characteristic component contents of the division plants, and selecting peony varieties that are not prone to degeneration.

9. Use of the multivariate polymorphism breeding method of peony seeds as described in any one of claims 1 to 8 in peony seed breeding.