Composition and application thereof in preparation of cosmetics with whitening effect

Abstract

The invention provides a composition and application thereof in preparation of cosmetics with a whitening effect. The composition is prepared from the following components in percentage by mass: 15 to 30 percent of taurine, 3 to 13 percent of hydroxyethyl piperazine ethanesulfonic acid, 8 to 15 percent of azelaic acid MEA, 3 to 13 percent of inositol, 0 to 5 percent of tranexamic acid, 1 to 5 percent of hydroxypropyl cyclodextrin, 0 to 1 percent of glutathione and the balance of nicotinamide. The composition jointly acts on multiple layers of skin through action mechanisms of different components, generation and transfer of melanin can be reduced, renewal and repair of the skin can be promoted, and therefore the whitening effect is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a composition and application thereof in preparing cosmetics with whitening effect. Background Art

[0002] The color of a person's skin color, hair color, and glasses is mainly related to the type, quantity, and distribution of melanin synthesized by melanocytes. The color of human skin does not depend on the number of melanocytes, but on the number, size, distribution, and degree of melanization of melanosomes. Unhealthy lifestyles can cause endocrine disorders, abnormal pigmentation, and spots on the skin. In modern society, people live a fast-paced life with high pressure, and coupled with environmental influences, the skin often has various problems, and cosmetics are needed to repair the skin. Most people believe that the formation of facial freckles, chloasma, and age spots is one of the main factors that affect appearance and self-confidence.

[0003] At present, there are endless freckle removal products on the cosmetics market, but most of them contain a variety of preservatives, ethanol, synthetic flavors and fragrances, etc. The presence of too many non-functional ingredients will cause additional damage to the skin, such as skin allergies, redness and swelling. In addition, the freckle removal and whitening products currently on the market may have a certain effect in the short term, but long-term use will cause the skin to tolerate and rebound, and the effect will deteriorate. Summary of the invention

[0004] The purpose of the present invention is to provide a composition and application thereof in preparing cosmetics with whitening effect.

[0005] In order to achieve the above object, the present invention provides the following technical solutions: A composition comprises the following components in percentage by mass: 15-30% taurine, 3-13% hydroxyethylpiperazineethanesulfonic acid, 8-15% azelaic acid MEA, 3-13% inositol, 0-5% tranexamic acid, 1-5% hydroxypropyl cyclodextrin, 0-1% glutathione, and the balance being nicotinamide.

[0006] Furthermore, the preparation method of hydroxypropyl cyclodextrin comprises the following steps: (1) 8-12 parts by weight of calcium hydroxide, 500 parts by weight of deionized water, and 52-58 parts by weight of cyclodextrin are mixed and stirred evenly, the temperature is raised to 30-35° C., and 20-25 parts by weight of 1,2-propylene oxide is slowly added dropwise for 10-15 hours. After the addition is completed, stirring is continued for 15-20 hours to obtain a reaction solution; (2) adding carbon dioxide gas dropwise to the reaction solution to adjust the pH value to 7.5-7.8, and allowing the solution to stand for 24 hours to fully precipitate, thereby obtaining a precipitate-containing solution; (3) filtering the precipitate-containing solution to remove insoluble matter to obtain a feed solution; (4) The feed liquid is introduced into a rotary evaporator and evaporated to dryness at 75-78°C to obtain hydroxypropyl cyclodextrin.

[0007] Furthermore, the cyclodextrin is a mixture of α-cyclodextrin and β-cyclodextrin in a mass ratio of 2-3:1.

[0008] Furthermore, the preparation method of glutathione comprises the following steps: (1) The fermentation liquid is inoculated with brewer's yeast at an inoculation amount of 3% to 5%, the fermentation temperature is 25 to 30°C, and the fermentation time is 12 to 15 hours to obtain brewer's yeast fermentation liquid; (2) treating a fermentation broth of Saccharomyces cerevisiae with low-temperature plasma, causing the Saccharomyces cerevisiae to enter a sublethal damage state, thereby obtaining a primary fermentation broth; wherein the number of Saccharomyces cerevisiae in the sublethal damage state accounts for 63% to 65% of the total number of live bacteria in the primary fermentation broth; (3) placing the primary fermentation liquid in a fermentation reactor at 25-30° C. for storage and fermentation for 20-23 hours to obtain a secondary fermentation liquid; in the secondary fermentation liquid, the number of Saccharomyces cerevisiae in a sublethal damage state accounts for 74%-75% of the total number of live Saccharomyces cerevisiae; (4) The secondary fermentation bacterial liquid is crushed and purified to obtain glutathione.

[0009] Furthermore, the working voltage of the low-temperature plasma is 45-50 kV, and the processing time is 5-8 min.

[0010] Furthermore, the gas source of the low-temperature plasma is sterile air.

[0011] Furthermore, the brewer's yeast is a mixture of brewer's yeast SHBCC D53785, brewer's yeast SHBCCD53787, and brewer's yeast SHBCCD53790 in a mass ratio of 1:1.2-1.3:0.3-0.6.

[0012] The brewer's yeast used in the present invention is a commercially available product purchased from Shanghai Microbiological Collection Center.

[0013] Furthermore, the specific method of crushing and purifying is: a. Use acid-heat extraction method to break the cell wall of brewer's yeast in the secondary fermentation liquid; b. Using a filter membrane to remove impurities in the fermentation broth after the crushing treatment in step a to obtain a concentrate; c. Crystallize and dry the concentrated solution to obtain glutathione.

[0014] Furthermore, the filter membrane is a filter membrane with a pore size of 0.22 μm.

[0015] Furthermore, the preparation method of the composition comprises the following steps: (1) Mix nicotinamide, taurine, hydroxyethylpiperazineethane sulfonic acid, inositol, and tranexamic acid, and stir at 30-35° C. for 15-20 minutes to obtain a mixture A; (2) Mixing MEA, hydroxypropyl cyclodextrin and glutathione at 40-45° C. for 20-25 min to obtain a mixture B; (3) Mixing mixture A and mixture B, stirring at 30-35° C. for 15-20 min, and cooling to room temperature to obtain a composition.

[0016] The composition is used in preparing cosmetics with whitening effect.

[0017] The invention discloses an application of a composition in preparing cosmetics with whitening effect.

[0018] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. The composition provided by the present invention comprises a plurality of ingredients, each of which has a certain whitening effect or auxiliary whitening effect, and the synergistic effect between each other makes the composition have a good whitening effect. Taurine can help improve the health of the skin and promote metabolism, thereby indirectly improving the brightness of the skin color. Hydroxyethylpiperazineethanesulfonic acid (HEPES): HEPES is a mild pH regulator that helps maintain the acid-base balance on the skin surface and promotes the effective absorption of other whitening ingredients. Azelaic acid MEA is a derivative of niacinamide that can reduce the transfer of melanin, thereby reducing spots and uneven skin color. Hydroxypropyl cyclodextrin can improve the stability and permeability of other whitening ingredients, thereby enhancing the effect of the entire formula. Glutathione is a powerful antioxidant that can reduce pigmentation caused by oxidative stress and help improve skin color. Niacinamide is a derivative of vitamin B3, which can not only reduce the production of melanin, but also enhance the skin barrier function and reduce inflammation, thereby improving uneven skin color.

[0019] The combination works together on multiple levels of the skin through the mechanisms of action of different ingredients, which can not only reduce the production and transfer of melanin, but also promote skin renewal and repair, thereby achieving a whitening effect.

[0020] 2. Hydroxypropyl cyclodextrin is a cyclic oligosaccharide, and its main function is to increase the solubility and stability of other ingredients, thereby improving their bioavailability. Certain impurities contained in commercially available hydroxypropyl cyclodextrin may interfere with the interaction between hydroxypropyl cyclodextrin and whitening ingredients, reducing the effective release of whitening ingredients. Certain impurities may cause irritation or allergic reactions to the skin, affecting the overall health of the skin. Impurities may change the physicochemical properties of hydroxypropyl cyclodextrin, affect its stability in the formula, and then affect the stability of whitening ingredients. Commercially available glutathione contains impurities, which will weaken the antioxidant properties of glutathione and reduce its inhibitory effect on melanin production. Impurities may affect the stability of glutathione, making it more easily degraded, thereby reducing its whitening effect. The hydroxypropyl cyclodextrin and glutathione prepared by the present invention by a special method have few impurities and high purity, ensuring that the product is safe and effective, and achieving the expected whitening effect. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] Example 1 The present embodiment provides a composition, which comprises the following components in percentage by mass: 20% taurine, 7% hydroxyethylpiperazine ethane sulfonic acid, 11% azelaic acid MEA, 6% inositol, 2.5% tranexamic acid, 3% hydroxypropyl cyclodextrin, 0.5% glutathione, and the balance niacinamide.

[0023] The preparation method of hydroxypropyl cyclodextrin comprises the following steps: (1) 8 parts by weight of calcium hydroxide, 500 parts by weight of deionized water, and 52 parts by weight of cyclodextrin were mixed and stirred evenly, the mixture was heated to 30° C., and 20 parts by weight of 1,2-propylene oxide was slowly added dropwise over a period of 10 hours. After the addition was completed, stirring was continued for 15 hours to obtain a reaction solution; (2) adding carbon dioxide gas dropwise to the reaction solution to adjust the pH value to 7.5, and allowing the solution to stand for 24 hours to fully precipitate, thereby obtaining a precipitate-containing solution; (3) filtering the precipitate-containing solution to remove insoluble matter to obtain a feed solution; (4) The feed liquid is introduced into a rotary evaporator and evaporated to dryness at 75°C to obtain hydroxypropyl cyclodextrin.

[0024] The cyclodextrin is a mixture of α-cyclodextrin and β-cyclodextrin in a mass ratio of 2:1.

[0025] The preparation method of glutathione comprises the following steps: (1) The fermentation liquid is inoculated with brewer's yeast at an inoculation amount of 3%, the fermentation temperature is 25°C, and the fermentation time is 12 hours to obtain brewer's yeast fermentation liquid; (2) treating a fermentation broth of Saccharomyces cerevisiae with low-temperature plasma, causing the Saccharomyces cerevisiae to enter a sublethal damage state, thereby obtaining a primary fermentation broth; wherein the number of Saccharomyces cerevisiae in the sublethal damage state accounts for 63% of the total number of live bacteria in the primary fermentation broth; (3) placing the primary fermentation liquid in a fermentation reactor at 25° C. for storage and fermentation for 20 to 23 hours to obtain a secondary fermentation liquid; in the secondary fermentation liquid, the number of Saccharomyces cerevisiae in a sublethal damage state accounts for 74% of the total number of live Saccharomyces cerevisiae; (4) The secondary fermentation bacterial liquid is crushed and purified to obtain glutathione.

[0026] The working voltage of the low-temperature plasma is 45 kV, and the processing time is 5 min.

[0027] The gas source of the low-temperature plasma is sterile air.

[0028] The brewer's yeast is a mixture of brewer's yeast SHBCC D53785, brewer's yeast SHBCCD53787 and brewer's yeast SHBCCD53790 in a mass ratio of 1:1.2:0.3.

[0029] The brewer's yeast used in the present invention is a commercially available product purchased from Shanghai Microbiological Collection Center.

[0030] The specific method of the crushing and purification is: a. Use acid-heat extraction method to break the cell wall of brewer's yeast in the secondary fermentation liquid; b. Using a filter membrane to remove impurities in the fermentation broth after the crushing treatment in step a to obtain a concentrate; c. Crystallize and dry the concentrated solution to obtain glutathione.

[0031] The filter membrane has a pore size of 0.22 μm.

[0032] The preparation method of the composition comprises the following steps: (1) Mix nicotinamide, taurine, hydroxyethylpiperazineethane sulfonic acid, inositol, and tranexamic acid, and stir at 30° C. for 15 min to obtain a mixture A; (2) Mixing MEA, hydroxypropyl cyclodextrin and glutathione at 40° C. for 20 min to obtain a mixture B; (3) Mixture A and mixture B were mixed, stirred at 30° C. for 15 min, and cooled to room temperature to obtain a composition.

[0033] Example 2 The present embodiment provides a composition, which comprises the following components in percentage by mass: 15% taurine, 13% hydroxyethylpiperazine ethane sulfonic acid, 8% azelaic acid MEA, 13% inositol, 1% tranexamic acid, 2% hydroxypropyl cyclodextrin, 1% glutathione, and niacinamide as the balance.

[0034] The preparation method of hydroxypropyl cyclodextrin comprises the following steps: (1) 12 parts by weight of calcium hydroxide, 500 parts by weight of deionized water, and 58 parts by weight of cyclodextrin were mixed and stirred evenly, the mixture was heated to 30-35° C., and 25 parts by weight of 1,2-propylene oxide was slowly added dropwise over a period of 15 hours. After the addition was completed, stirring was continued for 20 hours to obtain a reaction solution; (2) adding carbon dioxide gas dropwise to the reaction solution to adjust the pH value to 7.8, and allowing the solution to stand for 24 hours to fully precipitate, thereby obtaining a precipitate-containing solution; (3) filtering the precipitate-containing solution to remove insoluble matter to obtain a feed solution; (4) The feed liquid is introduced into a rotary evaporator and evaporated to dryness at 78°C to obtain hydroxypropyl cyclodextrin.

[0035] The cyclodextrin is a mixture of α-cyclodextrin and β-cyclodextrin in a mass ratio of 3:1.

[0036] The preparation method of glutathione comprises the following steps: (1) The fermentation liquid is inoculated with brewer's yeast at an inoculation amount of 5%, the fermentation temperature is 30°C, and the fermentation time is 15 hours to obtain brewer's yeast fermentation liquid; (2) treating a fermentation broth of Saccharomyces cerevisiae with low-temperature plasma, causing the Saccharomyces cerevisiae to enter a sublethal damage state, thereby obtaining a primary fermentation broth; wherein the number of Saccharomyces cerevisiae in the sublethal damage state accounts for 65% of the total number of live bacteria in the primary fermentation broth; (3) placing the primary fermentation liquid in a fermentation reactor at 30° C. for storage and fermentation for 23 hours to obtain a secondary fermentation liquid; in the secondary fermentation liquid, the number of Saccharomyces cerevisiae in a sublethal damage state accounts for 75% of the total number of live Saccharomyces cerevisiae; (4) The secondary fermentation bacterial liquid is crushed and purified to obtain glutathione.

[0037] Furthermore, the working voltage of the low-temperature plasma is 50 kV, and the processing time is 8 min.

[0038] The gas source of the low-temperature plasma is sterile air.

[0039] The brewer's yeast is a mixture of brewer's yeast SHBCC D53785, brewer's yeast SHBCCD53787, and brewer's yeast SHBCCD53790 in a mass ratio of 1: 1.3: 0.6.

[0040] The brewer's yeast used in the present invention is a commercially available product purchased from Shanghai Microbiological Collection Center.

[0041] The specific method of the crushing and purification is: a. Use acid-heat extraction method to break the cell wall of brewer's yeast in the secondary fermentation liquid; b. Using a filter membrane to remove impurities in the fermentation broth after the crushing treatment in step a to obtain a concentrate; c. Crystallize and dry the concentrated solution to obtain glutathione.

[0042] The filter membrane has a pore size of 0.22 μm.

[0043] The preparation method of the composition comprises the following steps: (1) Mix nicotinamide, taurine, hydroxyethylpiperazineethane sulfonic acid, inositol, and tranexamic acid, and stir at 35° C. for 20 min to obtain a mixture A; (2) Mixing MEA, hydroxypropyl cyclodextrin and glutathione at 45° C. for 25 min to obtain a mixture B; (3) Mixture A and mixture B were mixed, stirred at 35° C. for 20 min, and cooled to room temperature to obtain a composition.

[0044] Comparative Example 1 The difference between this comparative example and Example 1 is that the cyclodextrin is a mixture of α-cyclodextrin and β-cyclodextrin in a mass ratio of 1:1.

[0045] A composition comprises the following components in percentage by mass: 20% taurine, 7% hydroxyethylpiperazineethanesulfonic acid, 11% azelaic acid MEA, 6% inositol, 2.5% tranexamic acid, 3% hydroxypropyl cyclodextrin, 0.5% glutathione, and the balance being nicotinamide.

[0046] The preparation method of hydroxypropyl cyclodextrin comprises the following steps: (1) 8 parts by weight of calcium hydroxide, 500 parts by weight of deionized water, and 52 parts by weight of cyclodextrin were mixed and stirred evenly, the mixture was heated to 30° C., and 20 parts by weight of 1,2-propylene oxide was slowly added dropwise over a period of 10 hours. After the addition was completed, stirring was continued for 15 hours to obtain a reaction solution; (2) adding carbon dioxide gas dropwise to the reaction solution to adjust the pH value to 7.5, and allowing the solution to stand for 24 hours to fully precipitate, thereby obtaining a precipitate-containing solution; (3) filtering the precipitate-containing solution to remove insoluble matter to obtain a feed solution; (4) The feed liquid is introduced into a rotary evaporator and evaporated to dryness at 75°C to obtain hydroxypropyl cyclodextrin.

[0047] The cyclodextrin is a mixture of α-cyclodextrin and β-cyclodextrin in a mass ratio of 1:1.

[0048] The preparation method of glutathione comprises the following steps: (1) The fermentation liquid is inoculated with brewer's yeast at an inoculation amount of 3%, the fermentation temperature is 25°C, and the fermentation time is 12 hours to obtain brewer's yeast fermentation liquid; (2) treating a fermentation broth of Saccharomyces cerevisiae with low-temperature plasma, causing the Saccharomyces cerevisiae to enter a sublethal damage state, thereby obtaining a primary fermentation broth; wherein the number of Saccharomyces cerevisiae in the sublethal damage state accounts for 63% of the total number of live bacteria in the primary fermentation broth; (3) placing the primary fermentation liquid in a fermentation reactor at 25° C. for storage and fermentation for 20 to 23 hours to obtain a secondary fermentation liquid; in the secondary fermentation liquid, the number of Saccharomyces cerevisiae in a sublethal damage state accounts for 74% of the total number of live Saccharomyces cerevisiae; (4) The secondary fermentation bacterial liquid is crushed and purified to obtain glutathione.

[0049] The working voltage of the low-temperature plasma is 45 kV, and the processing time is 5 min.

[0050] The gas source of the low-temperature plasma is sterile air.

[0051] The brewer's yeast is a mixture of brewer's yeast SHBCC D53785, brewer's yeast SHBCCD53787 and brewer's yeast SHBCCD53790 in a mass ratio of 1:1.2:0.3.

[0052] The brewer's yeast used in the present invention is a commercially available product purchased from Shanghai Microbiological Collection Center.

[0053] The specific method of the crushing and purification is: a. Use acid-heat extraction method to break the cell wall of brewer's yeast in the secondary fermentation liquid; b. Using a filter membrane to remove impurities in the fermentation broth after the crushing treatment in step a to obtain a concentrate; c. Crystallize and dry the concentrated solution to obtain glutathione.

[0054] The filter membrane has a pore size of 0.22 μm.

[0055] The preparation method of the composition comprises the following steps: (1) Mix nicotinamide, taurine, hydroxyethylpiperazineethane sulfonic acid, inositol, and tranexamic acid, and stir at 30° C. for 15 min to obtain a mixture A; (2) Mixing MEA, hydroxypropyl cyclodextrin and glutathione at 40° C. for 20 min to obtain a mixture B; (3) Mixture A and mixture B were mixed, stirred at 30° C. for 15 min, and cooled to room temperature to obtain a composition.

[0056] Comparative Example 2 The difference between this comparative example and Example 1 is that hydroxypropyl cyclodextrin is a commercially available product purchased from Shandong Tongwang Biotechnology Co., Ltd.

[0057] A composition comprises the following components in percentage by mass: 20% taurine, 7% hydroxyethylpiperazineethanesulfonic acid, 11% azelaic acid MEA, 6% inositol, 2.5% tranexamic acid, 3% hydroxypropyl cyclodextrin, 0.5% glutathione, and the balance being nicotinamide.

[0058] The preparation method of glutathione comprises the following steps: (1) The fermentation liquid is inoculated with brewer's yeast at an inoculation amount of 3%, the fermentation temperature is 25°C, and the fermentation time is 12 hours to obtain brewer's yeast fermentation liquid; (2) treating a fermentation broth of Saccharomyces cerevisiae with low-temperature plasma, causing the Saccharomyces cerevisiae to enter a sublethal damage state, thereby obtaining a primary fermentation broth; wherein the number of Saccharomyces cerevisiae in the sublethal damage state accounts for 63% of the total number of live bacteria in the primary fermentation broth; (3) placing the primary fermentation liquid in a fermentation reactor at 25° C. for storage and fermentation for 20 to 23 hours to obtain a secondary fermentation liquid; in the secondary fermentation liquid, the number of Saccharomyces cerevisiae in a sublethal damage state accounts for 74% of the total number of live Saccharomyces cerevisiae; (4) The secondary fermentation bacterial liquid is crushed and purified to obtain glutathione.

[0059] The working voltage of the low-temperature plasma is 45 kV, and the processing time is 5 min.

[0060] The gas source of the low-temperature plasma is sterile air.

[0061] The brewer's yeast is a mixture of brewer's yeast SHBCC D53785, brewer's yeast SHBCCD53787 and brewer's yeast SHBCCD53790 in a mass ratio of 1:1.2:0.3.

[0062] The brewer's yeast used in the present invention is a commercially available product purchased from Shanghai Microbiological Collection Center.

[0063] The specific method of the crushing and purification is: a. Use acid-heat extraction method to break the cell wall of brewer's yeast in the secondary fermentation liquid; b. Using a filter membrane to remove impurities in the fermentation broth after the crushing treatment in step a to obtain a concentrate; c. Crystallize and dry the concentrated solution to obtain glutathione.

[0064] The filter membrane has a pore size of 0.22 μm.

[0065] The preparation method of the composition comprises the following steps: (1) Mix nicotinamide, taurine, hydroxyethylpiperazineethane sulfonic acid, inositol, and tranexamic acid, and stir at 30° C. for 15 min to obtain a mixture A; (2) Mixing MEA, hydroxypropyl cyclodextrin and glutathione at 40° C. for 20 min to obtain a mixture B; (3) Mixture A and mixture B were mixed, stirred at 30° C. for 15 min, and cooled to room temperature to obtain a composition.

[0066] Comparative Example 3 The difference between this comparative example and Example 1 is that the brewer's yeast is a mixture of brewer's yeast SHBCCD53785, brewer's yeast SHBCCD53787 and brewer's yeast SHBCCD53790 in a mass ratio of 1:1:1.

[0067] A composition comprises the following components in percentage by mass: 20% taurine, 7% hydroxyethylpiperazineethanesulfonic acid, 11% azelaic acid MEA, 6% inositol, 2.5% tranexamic acid, 3% hydroxypropyl cyclodextrin, 0.5% glutathione, and the balance being nicotinamide.

[0068] The preparation method of hydroxypropyl cyclodextrin comprises the following steps: (1) 8 parts by weight of calcium hydroxide, 500 parts by weight of deionized water, and 52 parts by weight of cyclodextrin were mixed and stirred evenly, the mixture was heated to 30° C., and 20 parts by weight of 1,2-propylene oxide was slowly added dropwise over a period of 10 hours. After the addition was completed, stirring was continued for 15 hours to obtain a reaction solution; (2) adding carbon dioxide gas dropwise to the reaction solution to adjust the pH value to 7.5, and allowing the solution to stand for 24 hours to fully precipitate, thereby obtaining a precipitate-containing solution; (3) filtering the precipitate-containing solution to remove insoluble matter to obtain a feed solution; (4) The feed liquid is introduced into a rotary evaporator and evaporated to dryness at 75°C to obtain hydroxypropyl cyclodextrin.

[0069] The cyclodextrin is a mixture of α-cyclodextrin and β-cyclodextrin in a mass ratio of 2:1.

[0070] The preparation method of glutathione comprises the following steps: (1) The fermentation liquid is inoculated with brewer's yeast at an inoculation amount of 3%, the fermentation temperature is 25°C, and the fermentation time is 12 hours to obtain brewer's yeast fermentation liquid; (2) treating a fermentation broth of Saccharomyces cerevisiae with low-temperature plasma, causing the Saccharomyces cerevisiae to enter a sublethal damage state, thereby obtaining a primary fermentation broth; wherein the number of Saccharomyces cerevisiae in the sublethal damage state accounts for 63% of the total number of live bacteria in the primary fermentation broth; (3) placing the primary fermentation liquid in a fermentation reactor at 25° C. for storage and fermentation for 20 to 23 hours to obtain a secondary fermentation liquid; in the secondary fermentation liquid, the number of Saccharomyces cerevisiae in a sublethal damage state accounts for 74% of the total number of live Saccharomyces cerevisiae; (4) The secondary fermentation bacterial liquid is crushed and purified to obtain glutathione.

[0071] The working voltage of the low-temperature plasma is 45 kV, and the processing time is 5 min.

[0072] The gas source of the low-temperature plasma is sterile air.

[0073] The brewer's yeast is a mixture of brewer's yeast SHBCC D53785, brewer's yeast SHBCCD53787 and brewer's yeast SHBCCD53790 in a mass ratio of 1:1:1.

[0074] The brewer's yeast used in the present invention is a commercially available product purchased from Shanghai Microbiological Collection Center.

[0075] The specific method of the crushing and purification is: a. Use acid-heat extraction method to break the cell wall of brewer's yeast in the secondary fermentation liquid; b. Using a filter membrane to remove impurities in the fermentation broth after the crushing treatment in step a to obtain a concentrate; c. Crystallize and dry the concentrated solution to obtain glutathione.

[0076] The filter membrane has a pore size of 0.22 μm.

[0077] The preparation method of the composition comprises the following steps: (1) Mix nicotinamide, taurine, hydroxyethylpiperazineethane sulfonic acid, inositol, and tranexamic acid, and stir at 30° C. for 15 min to obtain a mixture A; (2) Mixing MEA, hydroxypropyl cyclodextrin and glutathione at 40° C. for 20 min to obtain a mixture B; (3) Mixture A and mixture B were mixed, stirred at 30° C. for 15 min, and cooled to room temperature to obtain a composition.

[0078] Comparative Example 4 The difference between this comparative example and Example 1 is that glutathione is a commercially available product purchased from Shandong Baiqianhui Biotechnology Co., Ltd.

[0079] A composition comprises the following components in percentage by mass: 20% taurine, 7% hydroxyethylpiperazineethanesulfonic acid, 11% azelaic acid MEA, 6% inositol, 2.5% tranexamic acid, 3% hydroxypropyl cyclodextrin, 0.5% glutathione, and the balance being nicotinamide.

[0080] The preparation method of hydroxypropyl cyclodextrin comprises the following steps: (1) 8 parts by weight of calcium hydroxide, 500 parts by weight of deionized water, and 52 parts by weight of cyclodextrin were mixed and stirred evenly, the mixture was heated to 30° C., and 20 parts by weight of 1,2-propylene oxide was slowly added dropwise over a period of 10 hours. After the addition was completed, stirring was continued for 15 hours to obtain a reaction solution; (2) adding carbon dioxide gas dropwise to the reaction solution to adjust the pH value to 7.5, and allowing the solution to stand for 24 hours to fully precipitate, thereby obtaining a precipitate-containing solution; (3) filtering the precipitate-containing solution to remove insoluble matter to obtain a feed solution; (4) The feed liquid is introduced into a rotary evaporator and evaporated to dryness at 75°C to obtain hydroxypropyl cyclodextrin.

[0081] The cyclodextrin is a mixture of α-cyclodextrin and β-cyclodextrin in a mass ratio of 2:1.

[0082] The preparation method of the composition comprises the following steps: (1) Mix nicotinamide, taurine, hydroxyethylpiperazineethane sulfonic acid, inositol, and tranexamic acid, and stir at 30° C. for 15 min to obtain a mixture A; (2) Mixing MEA, hydroxypropyl cyclodextrin and glutathione at 40° C. for 20 min to obtain a mixture B; (3) Mixture A and mixture B were mixed, stirred at 30° C. for 15 min, and cooled to room temperature to obtain a composition.

[0083] Comparative Example 5 The difference between this comparative example and Example 1 is that the composition ratio is different.

[0084] A composition comprises the following components in percentage by mass: 10% taurine, 17% hydroxyethylpiperazineethanesulfonic acid, 6% azelaic acid MEA, 6% inositol, 7.5% tranexamic acid, 2% hydroxypropyl cyclodextrin, 1.5% glutathione, and the balance being nicotinamide.

[0085] The preparation method of hydroxypropyl cyclodextrin comprises the following steps: (1) 8 parts by weight of calcium hydroxide, 500 parts by weight of deionized water, and 52 parts by weight of cyclodextrin were mixed and stirred evenly, the mixture was heated to 30° C., and 20 parts by weight of 1,2-propylene oxide was slowly added dropwise over a period of 10 hours. After the addition was completed, stirring was continued for 15 hours to obtain a reaction solution; (2) adding carbon dioxide gas dropwise to the reaction solution to adjust the pH value to 7.5, and allowing the solution to stand for 24 hours to fully precipitate, thereby obtaining a precipitate-containing solution; (3) filtering the precipitate-containing solution to remove insoluble matter to obtain a feed solution; (4) The feed liquid is introduced into a rotary evaporator and evaporated to dryness at 75°C to obtain hydroxypropyl cyclodextrin.

[0086] The cyclodextrin is a mixture of α-cyclodextrin and β-cyclodextrin in a mass ratio of 2:1.

[0087] The preparation method of glutathione comprises the following steps: (1) The fermentation liquid is inoculated with brewer's yeast at an inoculation amount of 3%, the fermentation temperature is 25°C, and the fermentation time is 12 hours to obtain brewer's yeast fermentation liquid; (2) treating a fermentation broth of Saccharomyces cerevisiae with low-temperature plasma, causing the Saccharomyces cerevisiae to enter a sublethal damage state, thereby obtaining a primary fermentation broth; wherein the number of Saccharomyces cerevisiae in the sublethal damage state accounts for 63% of the total number of live bacteria in the primary fermentation broth; (3) placing the primary fermentation liquid in a fermentation reactor at 25° C. for storage and fermentation for 20 to 23 hours to obtain a secondary fermentation liquid; in the secondary fermentation liquid, the number of Saccharomyces cerevisiae in a sublethal damage state accounts for 74% of the total number of live Saccharomyces cerevisiae; (4) The secondary fermentation bacterial liquid is crushed and purified to obtain glutathione.

[0088] The working voltage of the low-temperature plasma is 45 kV, and the processing time is 5 min.

[0089] The gas source of the low-temperature plasma is sterile air.

[0090] The brewer's yeast is a mixture of brewer's yeast SHBCC D53785, brewer's yeast SHBCCD53787 and brewer's yeast SHBCCD53790 in a mass ratio of 1:1.2:0.3.

[0091] The brewer's yeast used in the present invention is a commercially available product purchased from Shanghai Microbiological Collection Center.

[0092] The specific method of the crushing and purification is: a. Use acid-heat extraction method to break the cell wall of brewer's yeast in the secondary fermentation liquid; b. Using a filter membrane to remove impurities in the fermentation broth after the crushing treatment in step a to obtain a concentrate; c. Crystallize and dry the concentrated solution to obtain glutathione.

[0093] The filter membrane has a pore size of 0.22 μm.

[0094] The preparation method of the composition comprises the following steps: (1) Mix nicotinamide, taurine, hydroxyethylpiperazineethane sulfonic acid, inositol, and tranexamic acid, and stir at 30° C. for 15 min to obtain a mixture A; (2) Mixing MEA, hydroxypropyl cyclodextrin and glutathione at 40° C. for 20 min to obtain a mixture B; (3) Mixture A and mixture B were mixed, stirred at 30° C. for 15 min, and cooled to room temperature to obtain a composition.

[0095] Performance Testing 70 male test subjects aged 30-40 with dull skin and acne marks were randomly selected and divided into 7 groups. The same area of ​​the test subject's face was selected as the test area. The mask was applied once every night. No other cosmetics could be used on the test area during the test. The mask was used for 2 months continuously. The average melanin value of the test area was tested using a skin melanin and hemoglobin tester on the 0th, 30th and 60th day of use. The smaller the value, the less melanin content. The results are shown in Table 1.

[0096] Table 1 Performance test results

[0097] It can be seen from the above performance test results that Examples 1-2 have good whitening effects, which is mainly due to the synergistic effect of various components. However, since the comparative example does not adopt the necessary technical solution, it is obviously inferior to the embodiment in the corresponding performance test. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effect.

[0098] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition, characterized in that The composition comprises the following components in percentage by mass: 15-30% taurine, 3-13% hydroxyethylpiperazineethanesulfonic acid, 8-15% azelaic acid MEA, 3-13% inositol, 0-5% tranexamic acid, 1-5% hydroxypropyl cyclodextrin, 0-1% glutathione, and the balance is nicotinamide.

2. The composition according to claim 1, characterized in that The preparation method of hydroxypropyl cyclodextrin comprises the following steps: (1) 8-12 parts by weight of calcium hydroxide, 500 parts by weight of deionized water, and 52-58 parts by weight of cyclodextrin are mixed and stirred evenly, the temperature is raised to 30-35° C., and 20-25 parts by weight of 1,2-propylene oxide is slowly added dropwise for 10-15 hours. After the addition is completed, stirring is continued for 15-20 hours to obtain a reaction solution; (2) adding carbon dioxide gas dropwise to the reaction solution to adjust the pH value to 7.5-7.8, and allowing the solution to stand for 24 hours to fully precipitate, thereby obtaining a precipitate-containing solution; (3) filtering the precipitate-containing solution to remove insoluble matter to obtain a feed solution; (4) The feed liquid is introduced into a rotary evaporator and evaporated to dryness at 75-78°C to obtain hydroxypropyl cyclodextrin.

3. The composition according to claim 2, characterized in that The cyclodextrin is a mixture of α-cyclodextrin and β-cyclodextrin in a mass ratio of 2-3:

1.

4. The composition according to claim 1, characterized in that The preparation method of glutathione comprises the following steps: (1) The fermentation liquid is inoculated with brewer's yeast at an inoculation amount of 3% to 5%, the fermentation temperature is 25 to 30°C, and the fermentation time is 12 to 15 hours to obtain brewer's yeast fermentation liquid; (2) treating a fermentation broth of Saccharomyces cerevisiae with low-temperature plasma, causing the Saccharomyces cerevisiae to enter a sublethal damage state, thereby obtaining a primary fermentation broth; wherein the number of Saccharomyces cerevisiae in the sublethal damage state accounts for 63% to 65% of the total number of live bacteria in the primary fermentation broth; (3) placing the primary fermentation liquid in a fermentation reactor at 25-30° C. for storage and fermentation for 20-23 hours to obtain a secondary fermentation liquid; in the secondary fermentation liquid, the number of Saccharomyces cerevisiae in a sublethal damage state accounts for 74%-75% of the total number of live Saccharomyces cerevisiae; (4) The secondary fermentation bacterial liquid is crushed and purified to obtain glutathione.

5. The composition according to claim 4, characterized in that The working voltage of the low-temperature plasma is 45-50 kV, and the processing time is 5-8 min.

6. The composition according to claim 5, characterized in that The preparation method of the composition comprises the following steps: the gas source of the low-temperature plasma is sterile air.

7. The composition according to claim 4, characterized in that The specific method of the crushing and purification is: a. Use acid-heat extraction method to break the cell wall of brewer's yeast in the secondary fermentation liquid; b. Using a filter membrane to remove impurities in the fermentation broth after the crushing treatment in step a to obtain a concentrate; c. Crystallize and dry the concentrated solution to obtain glutathione.

8. The composition according to claim 7, characterized in that The filter membrane has a pore size of 0.22 μm.

9. The composition according to claim 1, characterized in that The preparation method of the composition comprises the following steps: (1) Mix nicotinamide, taurine, hydroxyethylpiperazineethane sulfonic acid, inositol, and tranexamic acid, and stir at 30-35° C. for 15-20 minutes to obtain a mixture A; (2) Mixing MEA, hydroxypropyl cyclodextrin and glutathione at 40-45° C. for 20-25 min to obtain a mixture B; (3) Mixing mixture A and mixture B, stirring at 30-35° C. for 15-20 min, and cooling to room temperature to obtain a composition.

10. Use of the composition according to any one of claims 1 to 9 in preparing cosmetics with whitening effect.

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