Cell culture reactor

Abstract

The invention discloses a cell culture reactor, and relates to the technical field of cell culture.The cell culture reactor comprises a culture reaction mechanism and a second supporting column installed at the top of the culture reaction mechanism, a reaction vessel fixing mechanism is arranged in the culture reaction mechanism, and a reaction vessel body is arranged at the top of the reaction vessel fixing mechanism; the culture reaction mechanism comprises a culture working table, first supporting columns are fixedly installed on the periphery of the bottom of the culture working table, a supporting bottom plate is fixedly installed at the bottoms of the first supporting columns, supporting legs are fixedly installed on the periphery of the bottom of the supporting bottom plate, and a sealing groove is fixedly formed in the inner side of the bottom of the culture working table; due to the fact that the lengths of the vibration connecting rods are different, the movable rolling wheels can drive the placing plate to swing up and down in multiple directions while moving on the vibration wave ring, and multi-direction vibration of the placing plate can be achieved by means of the characteristic that a first spherical rotating support at the top of an inclined rotating rod is in sliding connection with an inclined sliding rod and an inclined sliding sleeve.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a cell culture reactor. Background Art

[0002] Cell culture refers to the process of making cells from tissues, organs or organisms proliferate and propagate continuously under artificial conditions in vitro in order to obtain enough cells of the same type for research. Cell culture plays an important role in life science research, such as cancer research, pharmacology, genetics, physiology and other fields. This technology requires the use of some basic equipment and containers, such as clean benches, biosafety cabinets, cell culture incubators, microscopes, cell culture plates, cell bottles and cell dishes. The selection of appropriate culture media and additives can provide the nutrients and growth factors required for cell proliferation and growth. Maintaining the temperature, humidity and carbon dioxide concentration of the culture environment is also very important for cell growth and expansion.

[0003] Publication No. CN114703058A discloses a cell culture reactor, in which a threaded connection with a locking bolt is symmetrically arranged on the surface of a positioning sleeve, and the locking bolt is manually rotated. One end of the locking bolt passes through the positioning sleeve and is rotatably connected to the surface of one side of the arc plate through a bearing, so that the inner arc surfaces of the two arc plates are abutted against the outer surface of the reaction vessel, and at the same time, the rubber pad bonded to the inner arc surface of the arc plate can improve the stability of the reaction vessel clamped by the two arc plates. At the same time, two guide rods fixedly installed on the surface of the arc plate are slidably connected to the guide holes opened on the surface of the positioning sleeve, so that the arc plate can be moved under the action of the locking bolt. The invention can stably move toward the surface of the reaction dish, so that the reaction dish can be fixed more conveniently; at the same time, a support component is added on this basis, and the motor is controlled to start, and then the cam fixedly installed on the output shaft of the motor cooperates with the centrifugal force generated by the rotation of the cam, so that the fixed component on the surface of the reaction table shakes, so that the culture medium in the reaction dish shakes, so that the culture medium and cells are fully mixed, and the proliferation of cells is accelerated. Through the cooperation of the guide sleeve and the spring, the centrifugal force generated by the rotation of the cam can be absorbed and fed back, thereby improving the stability of the reactor. However, the patent still has the following problems in actual use:

[0004] Although the cell culture reactor is started by controlling the motor, and then with the cooperation of the cam fixedly mounted on the output shaft of the motor, the centrifugal force generated by the rotation of the cam causes the fixed components on the surface of the reaction table to shake, thereby causing the culture fluid in the reaction dish to shake and fully mix the culture fluid and cells, the effect of mixing the culture fluid and cells by shaking is limited. When the rotation speed of the cam cannot be accurately controlled, the reaction dish will vibrate violently, thereby causing damage to the cells, which is not conducive to sufficient mixing of the cells and the culture fluid.

[0005] Therefore, a cell culture reactor is proposed to solve the above problems. Summary of the invention

[0006] The object of the present invention is to provide a cell culture reactor to solve the problem that although the cell culture reactor proposed in the above background technology is started by controlling the motor, and then with the cooperation of the cam fixedly installed on the output shaft of the motor, the centrifugal force generated by the rotation of the cam causes the fixed components on the surface of the reaction table to shake, thereby causing the culture fluid in the reaction dish to shake and fully mix the culture fluid and cells, the effect of mixing the culture fluid and cells by shaking is limited. When the rotation speed of the cam cannot be accurately controlled, the reaction dish will vibrate violently, thereby causing damage to the cells, which is not conducive to the full mixing of the cells and the culture fluid.

[0007] To achieve the above object, the present invention provides the following technical solution: a cell culture reactor, comprising a culture reaction mechanism, and a second support column installed on the top of the culture reaction mechanism;

[0008] A reaction vessel fixing mechanism is arranged inside the culture reaction mechanism, and a reaction vessel body is arranged on the top of the reaction vessel fixing mechanism;

[0009] Also includes:

[0010] The culture reaction mechanism comprises a culture workbench, the bottom of which is fixedly mounted with first support columns around the periphery, and the bottom of which is fixedly mounted with a support base plate;

[0011] Among them, support legs are fixedly installed around the bottom of the supporting bottom plate, and a sealing groove is fixedly installed on the inner side of the bottom of the culture workbench;

[0012] Wherein, a cylinder is fixedly installed at the bottom of the sealing groove, and the cylinder is fixedly installed on the top of the supporting bottom plate.

[0013] Preferably, a first fixed sleeve is fixedly installed at the top center position of the support base plate, a rotating motor is fixedly installed at the bottom center position of the support base plate, a meshing gear ring is fixedly installed inside the first fixed sleeve, the output end of the rotating motor is fixedly connected to a rotating main gear, the outer inner ring of the rotating main gear is connected to a rotating driven gear, and the rotating driven gear is meshingly connected to the meshing gear ring.

[0014] Preferably, the top of the driven gear is rotatably connected to a rotating disk, a vibrating wave ring is fixedly installed on the outer side of the top of the rotating disk, a rotating connecting rod is fixedly installed on the top of the rotating main gear, the top of the rotating connecting rod is fixedly connected to a supporting disk, a plurality of rotating grooves are opened inside the supporting disk, and an inclined sliding rod is fixedly installed inside the rotating groove.

[0015] Preferably, the outer side of the tilted sliding rod is slidingly connected to a tilted sliding sleeve, one side of the tilted sliding sleeve is fixedly connected to a tilted spring, the top of the tilted sliding sleeve is rotatably connected to a tilted rotating rod, the top of the tilted rotating rod is rotatably connected to a first spherical rotating support, a placement plate is fixedly installed on the top of the first spherical rotating support, and second spherical rotating supports are fixedly installed around the bottom of the placement plate.

[0016] Preferably, the bottom of the second spherical rotating support is rotatably connected to a vibration connecting rod, the bottom of the vibration connecting rod is fixedly connected to a vibration spring, a spring telescopic rod is arranged inside the vibration spring, a movable roller is fixedly installed at the bottom of the spring telescopic rod, and the second support column is fixedly installed around the top of the culture workbench.

[0017] Preferably, a supporting top plate is fixedly installed on the top of the second supporting column, a lifting cylinder is fixedly installed on the bottom center position of the supporting top plate, the output end of the lifting cylinder is fixedly connected to a lifting plate, a lifting sliding sleeve is fixedly installed around the lifting plate, the lifting sliding sleeve is slidably connected to the second supporting column, a protective cover is fixedly installed on the bottom of the lifting plate, and a sealing ring is fixedly installed on the outer side of the bottom of the protective cover.

[0018] Preferably, the reaction vessel fixing mechanism includes a fixed base, a second fixed sleeve is fixedly installed at the inner center position of the fixed base, a clamping rotating rod is rotatably connected to the top of the fixed base, a clamping sliding sleeve is rotatably connected to the top of the clamping rotating rod, the inside of the clamping sliding sleeve is slidably connected to the clamping sliding rod, and a clamping spring is fixedly connected to one side of the clamping sliding sleeve.

[0019] Preferably, a clamping limit block is fixedly installed on the top of the clamping sliding sleeve, a deformable arc plate is fixedly installed on the top of the clamping limit block, a clamping bracket is fixedly installed on the outer side of the clamping sliding rod, the reaction vessel body is arranged on the inner side of the deformable arc plate, a vertical slot is opened at the inner center position of the second fixed sleeve, and limiting sliding grooves are symmetrically installed on both sides of the second fixed sleeve close to the vertical slot.

[0020] Preferably, the second fixed sleeve is provided with a fixed rotating groove near the bottom of the vertical slot and the limiting slide groove, a locking spring is symmetrically installed inside the fixed rotating groove, an opening and closing block is fixedly installed on the end of the locking spring, a rotating plate is rotatably connected to the top center position of the clamping spring, a connecting rod is fixedly connected to the bottom center position of the rotating plate, and fixed blocks are symmetrically installed on both sides of the bottom of the connecting rod.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: in this cell culture reactor, since the lengths of the vibration connecting rods are different, the movable roller can move on the vibration wave ring while driving the placement plate to swing up and down in multiple directions. By utilizing the characteristics of the sliding connection between the first spherical rotating support at the top of the inclined rotating rod and the inclined sliding rod and the inclined sliding sleeve, the placement plate can be vibrated in multiple directions. By pressing down the rotating plate, the connecting rod and the fixed block at the bottom of the rotating plate are respectively inserted into the vertical slot and the limiting groove inside the second fixed sleeve. When the clamping bracket descends, the clamping rotating rod can be driven to rotate, so that the clamping sliding sleeve slides on the outer side of the clamping sliding rod and drives the clamping limiting block and the deformable arc plate to move relative to each other. When the connecting rod and the fixed block move to the bottom of the vertical slot and the limiting groove, the deformable arc plate can clamp and limit the reaction vessel body. The specific contents are as follows:

[0022] 1. By setting up the culture reaction mechanism, not only can the rotating motor be used to drive the rotating main gear to rotate, but also the characteristics of the meshing connection between the rotating main gear, the driven gear and the meshing gear ring can be used to realize the rotation of the rotating disk, so that the vibration wave ring on the top of the rotating disk can be rotated. At the same time, the rotating main gear drives the rotating connecting rod and the supporting disk to rotate. Under the action of the inclined rotating rod and the first spherical rotating support, the rotation of the placing plate, the second spherical rotating support and the vibration connecting rod can be realized, so that the vibration connecting rod drives the vibration spring, the spring telescopic rod and the moving roller to rotate on the top of the vibration wave ring. Since the lengths of the vibration connecting rods are different, the moving roller can be The roller drives the placement plate to swing up and down in multiple directions while moving on the vibration wave ring. The placement plate can be vibrated in multiple directions by utilizing the first spherical rotation support at the top of the tilting rotation rod and the sliding connection between the tilting sliding rod and the tilting sliding sleeve. The culture fluid and cells inside the reaction dish body are fully mixed by rotation and up and down multi-directional swinging to accelerate cell proliferation. The lifting plate is driven by the lifting cylinder. Under the action of the sliding connection between the lifting sliding sleeve and the second support column, the protective cover drives the sealing ring to move up and down. When the sealing ring is fitted and connected to the sealing groove, the entire reactor can be closed to prevent the external environment from having a certain influence on the cell culture.

[0023] 2. By setting the reaction dish fixing mechanism, not only can the reaction dish body containing culture fluid and cells be placed on the top of the clamping bracket and the rotating plate, but also by pressing down the rotating plate, the connecting rod and the fixed block at the bottom of the rotating plate are respectively inserted into the vertical slot and the limiting slide groove inside the second fixed sleeve, and when the clamping bracket is lowered, the clamping rotating rod can be driven to rotate, so that the clamping sliding sleeve slides on the outer side of the clamping sliding rod and drives the clamping limiting block and the deformable arc plate to move relatively. When the connecting rod and the fixed block move to the bottom of the vertical slot and the limiting slide groove, the deformable arc plate can clamp and limit the reaction dish body. By rotating the reaction dish body, the rotating plate, the connecting rod and the fixed block are driven to rotate, so that the fixed block passes over the clamping spring and the opening and closing block for limiting and fixing, thereby the reaction dish body can be stably limited, the vibration and rotation stability of the reaction dish body is improved, and it is convenient to fully mix the culture fluid and the cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0025] Figure 2 It is a schematic diagram of the three-dimensional structure of the cross section of the culture reaction mechanism in the present invention;

[0026] Figure 3 It is a schematic diagram of the three-dimensional structure of the vibration wave ring in the present invention;

[0027] Figure 4 It is a schematic diagram of the three-dimensional structure of the rotating disk in the present invention;

[0028] Figure 5 It is a schematic diagram of the three-dimensional structure of the rotating connecting rod in the present invention;

[0029] Figure 6 It is a schematic diagram of the three-dimensional structure of the support plate in the present invention;

[0030] Figure 7 It is a schematic diagram of the three-dimensional structure of the tilting and rotating rod in the present invention;

[0031] Figure 8 It is a schematic diagram of the three-dimensional structure of the mobile roller in the present invention;

[0032] Fig. 9 It is a schematic diagram of the three-dimensional structure of the reaction vessel fixing mechanism in the present invention;

[0033] Fig.10 It is a schematic diagram of the three-dimensional structure of the cross section of the fixed base and the clamping bracket in the present invention;

[0034] Fig.11 It is a schematic diagram of the three-dimensional structure of the cross section of the second fixed sleeve in the present invention.

[0035] In the figure: 1, culture reaction mechanism; 101, culture workbench; 102, first support column; 103, support bottom plate; 104, support leg; 105, sealing groove; 106, cylinder; 107, first fixed sleeve; 108, rotating motor; 109, meshing gear ring; 110, rotating main gear; 111, rotating driven gear; 112, rotating disk; 113, vibrating wave ring; 114, rotating connecting rod; 115, supporting disk; 116, rotating slide; 117, tilting sliding rod; 118, tilting sliding sleeve; 119, tilting spring; 120, tilting rotating rod; 121, first spherical rotating support; 122, placing plate; 123, second spherical rotating support; 124, vibrating connecting rod; 125, vibrating spring; 126, spring Spring telescopic rod; 127, moving roller; 128, second support column; 129, support top plate; 130, lifting cylinder; 131, lifting plate; 132, lifting sliding sleeve; 133, protective cover; 134, sealing ring; 2, reaction vessel fixing mechanism; 201, fixed base; 202, second fixed sleeve; 203, clamping rotating rod; 204, clamping sliding sleeve; 205, clamping sliding rod; 206, clamping spring; 207, clamping limit block; 208, deformable arc plate; 209, clamping bracket; 210, reaction vessel body; 211, vertical slot; 212, limit slide groove; 213, fixed rotating groove; 214, locking spring; 215, opening and closing block; 216, rotating plate; 217, connecting rod; 218, fixed clamping block. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the implementation regulations described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] See also Figure 1-Figure 11The present invention provides a technical solution: a cell culture reactor, comprising a culture reaction mechanism 1, and a second support column 128 installed on the top of the culture reaction mechanism 1, a reaction vessel fixing mechanism 2 is arranged inside the culture reaction mechanism 1, and a reaction vessel body 210 is arranged on the top of the reaction vessel fixing mechanism 2, the culture reaction mechanism 1 comprises a culture workbench 101, a first support column 102 is fixedly installed around the bottom of the culture workbench 101, and a support bottom plate 103 is fixedly installed at the bottom of the first support column 102, wherein the support bottom plate 103 The bottom of the plate 103 is fixedly installed with support legs 104, and the bottom inner side of the culture workbench 101 is fixedly installed with a sealing groove 105, wherein the bottom of the sealing groove 105 is fixedly installed with a cylinder 106, and the cylinder 106 is fixedly installed on the top of the supporting bottom plate 103, and the top center of the supporting bottom plate 103 is fixedly installed with a first fixed sleeve 107, and the bottom center of the supporting bottom plate 103 is fixedly installed with a rotating motor 108, and the inside of the first fixed sleeve 107 is fixedly installed with a meshing gear ring 109, and the rotating motor 108 is fixedly installed with a meshing gear ring 109. The output end of 08 is fixedly connected with a rotating main gear 110, and the outer inner ring of the rotating main gear 110 is connected with a rotating driven gear 111, and the rotating driven gear 111 is meshed with the meshing gear ring 109. The top of the rotating driven gear 111 is rotatably connected with a rotating disk 112, and a vibrating wave ring 113 is fixedly installed on the top outer side of the rotating disk 112. A rotating connecting rod 114 is fixedly installed on the top of the rotating main gear 110, and a supporting disk 115 is fixedly connected to the top of the rotating connecting rod 114. The rotating motor 108 drives the rotating main gear 110 to rotate. The gear 110 rotates, and by utilizing the characteristics of the meshing connection between the rotating main gear 110, the rotating driven gear 111 and the meshing gear ring 109, the rotation of the rotating disk 112 can be realized, thereby realizing the rotation of the vibration wave ring 113 on the top of the rotating disk 112. At the same time, the rotating main gear 110 drives the rotating connecting rod 114 and the supporting disk 115 to rotate. Under the action of the inclined rotating rod 120 and the first spherical rotating support 121, the rotation of the placing plate 122, the second spherical rotating support 123 and the vibration connecting rod 124 can be realized.

[0038] The support plate 115 is provided with a plurality of rotating grooves 116 inside, and a tilting sliding rod 117 is fixedly installed inside the rotating groove 116, and a tilting sliding sleeve 118 is slidably connected to the outer side of the tilting sliding rod 117, and a tilting spring 119 is fixedly connected to one side of the tilting sliding sleeve 118, and a tilting rotating rod 120 is rotatably connected to the top of the tilting sliding sleeve 118, and a first spherical rotating support 121 is rotatably connected to the top of the tilting rotating rod 120, and a placing plate 122 is fixedly installed on the top of the first spherical rotating support 121, and a second spherical rotating support 123 is fixedly installed around the bottom of the placing plate 122, and a vibration connecting rod 124 is rotatably connected to the bottom of the second spherical rotating support 123, and a vibration spring 125 is fixedly connected to the bottom of the vibration connecting rod 124, and a spring telescopic rod 125 is provided inside the vibration spring 125. 26. A moving roller 127 is fixedly installed at the bottom of the spring telescopic rod 126, and the second support column 128 is fixedly installed around the top of the culture workbench 101, so that the vibration connecting rod 124 drives the vibration spring 125, the spring telescopic rod 126 and the moving roller 127 to rotate on the top of the vibration wave ring 113. Since the lengths of the vibration connecting rods 124 are different, the moving roller 127 can move on the vibration wave ring 113 and drive the placement plate 122 to swing up and down in multiple directions. By utilizing the sliding connection between the first spherical rotating support 121 at the top of the inclined rotating rod 120 and the inclined sliding rod 117 and the inclined sliding sleeve 118, the placement plate 122 can be vibrated, and the culture solution and cells in the reaction dish body 210 are fully mixed by rotation and up and down multi-directional swinging, thereby accelerating the proliferation of cells.

[0039] A supporting top plate 129 is fixedly installed on the top of the second supporting column 128, and a lifting cylinder 130 is fixedly installed at the bottom center of the supporting top plate 129. A lifting plate 131 is fixedly connected to the output end of the lifting cylinder 130, and a lifting sliding sleeve 132 is fixedly installed around the lifting plate 131. The lifting sliding sleeve 132 is slidably connected to the second supporting column 128. A protective cover 133 is fixedly installed on the bottom of the lifting plate 131, and a sealing ring 134 is fixedly installed on the bottom outer side of the protective cover 133. The lifting cylinder 130 drives the lifting plate 131 to slide under the action of the lifting sliding sleeve 132 and the second supporting column 128. The protective cover 133 drives the sealing ring 134 to move up and down. When the sealing ring 134 is fitly connected to the sealing groove 105, the entire reactor can be closed to prevent the external environment from having a certain impact on the cell culture.

[0040] The reaction vessel fixing mechanism 2 includes a fixed base 201, a second fixed sleeve 202 is fixedly installed at the inner center position of the fixed base 201, a clamping rotating rod 203 is rotatably connected to the top of the fixed base 201, a clamping sliding sleeve 204 is rotatably connected to the top of the clamping rotating rod 203, a clamping sliding rod 205 is slidably connected to the inside of the clamping sliding sleeve 204, a clamping spring 206 is fixedly connected to one side of the clamping sliding sleeve 204, a clamping limit block 207 is fixedly installed on the top of the clamping limit block 207, a deformable arc plate 208 is fixedly installed on the top of the clamping limit block 207, and a clamping bracket is fixedly installed on the outer side of the clamping sliding rod 205 209, the reaction vessel body 210 is arranged on the inner side of the deformable arc plate 208, and the reaction vessel body 210 containing culture fluid and cells can be placed on the top of the clamping bracket 209 and the rotating plate 216. By pressing down the rotating plate 216, the connecting rod 217 and the fixed block 218 at the bottom of the rotating plate 216 are respectively inserted into the vertical slot 211 and the limiting slide groove 212 inside the second fixed sleeve 202. When the clamping bracket 209 is lowered, the clamping rotating rod 203 can be driven to rotate, so that the clamping sliding sleeve 204 slides on the outer side of the clamping sliding rod 205, while driving the clamping limiting block 207 and the deformable arc plate 208 to move relative to each other.

[0041] A vertical slot 211 is provided at the inner center of the second fixed sleeve 202, and limiting slots 212 are symmetrically installed on both sides of the second fixed sleeve 202 near the vertical slot 211. A fixed rotating slot 213 is provided at the bottom of the second fixed sleeve 202 near the vertical slot 211 and the limiting slot 212, and a clamping spring 214 is symmetrically installed inside the fixed rotating slot 213, and an opening and closing block 215 is fixedly installed at the end of the clamping spring 214. A rotating plate 216 is rotatably connected to the top center of the clamping spring 206, and a connecting rod 217 is fixedly connected to the bottom center of the rotating plate 216. Fixed blocks 218 are symmetrically installed on both sides of the bottom. When the connecting rod 217 and the fixed block 218 move to the bottom of the vertical slot 211 and the limiting slide groove 212, the deformable arc plate 208 can clamp and limit the reaction dish body 210. By rotating the reaction dish body 210, the rotating plate 216, the connecting rod 217 and the fixed block 218 are driven to rotate, so that the fixed block 218 passes over the locking spring 214 and the opening and closing block 215 for limiting and fixing, so that the reaction dish body 210 can be stably limited, the vibration and rotation stability of the reaction dish body 210 is improved, and the culture medium and cells are fully mixed.

[0042] Working principle: Before using this cell culture reactor, you need to check the overall condition of the device to make sure it can work normally. Figure 1 - Fig.11As shown, first, the reaction vessel body 210 containing the culture medium and cells is placed on the top of the clamping bracket 209 and the rotating plate 216, and the rotating plate 216 is pressed down to make the connecting rod 217 and the fixed block 218 at the bottom of the rotating plate 216 respectively inserted into the vertical slot 211 and the limiting slide groove 212 inside the second fixed sleeve 202, and when the clamping bracket 209 is lowered, the clamping rotating rod 203 can be driven to rotate, so that the clamping sliding sleeve 204 slides on the outer side of the clamping sliding rod 205, and at the same time, the clamping limiting block 207 and the deformable arc plate 208 are driven to move relative to each other. When the connecting rod 217 and the fixed block 218 move to the bottom of the vertical slot 211 and the limiting groove 212, the deformable arc plate 208 can clamp and limit the reaction dish body 210. By rotating the reaction dish body 210, the rotating plate 216, the connecting rod 217 and the fixed block 218 are driven to rotate, so that the fixed block 218 passes over the locking spring 214 and the opening and closing block 215 for limiting and fixing, so that the reaction dish body 210 can be stably limited, the vibration and rotation stability of the reaction dish body 210 is improved, and the culture medium and cells are fully mixed.

[0043] Secondly, the rotating motor 108 is used to drive the rotating main gear 110 to rotate. By utilizing the meshing connection between the rotating main gear 110, the rotating driven gear 111 and the meshing gear ring 109, the rotating disk 112 can be rotated, thereby realizing the rotation of the vibration wave ring 113 on the top of the rotating disk 112. At the same time, the rotating main gear 110 drives the rotating connecting rod 114 and the supporting disk 115 to rotate. Under the action of the inclined rotating rod 120 and the first spherical rotating support 121, the rotation of the placing plate 122, the second spherical rotating support 123 and the vibration connecting rod 124 can be realized, so that the vibration connecting rod 124 drives the vibration spring 125, the spring telescopic rod 126 and the moving roller 127 to rotate on the top of the vibration wave ring 113. Since the lengths of the vibration connecting rods 124 are different, the moving roller 127 can move on the vibration wave ring 113 while driving the placing plate 122 to swing up and down in multiple directions.

[0044] Finally, by utilizing the characteristics of the sliding connection between the first spherical rotating support 121 at the top of the inclined rotating rod 120 and the inclined sliding rod 117 and the inclined sliding sleeve 118, the placement plate 122 can be vibrated, and the culture medium and cells inside the reaction vessel body 210 can be fully mixed by rotation and multi-directional swinging up and down, thereby accelerating the proliferation of cells. By setting the lifting cylinder 130 to drive the lifting plate 131, under the action of the sliding connection between the lifting sliding sleeve 132 and the second support column 128, the protective cover 133 drives the sealing ring 134 to move up and down. When the sealing ring 134 is fitted and connected with the sealing groove 105, the entire reactor can be closed to avoid certain influences of the external environment on the cell culture.

[0045] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell culture reactor, comprising a culture reaction mechanism (1), and a second support column (128) installed on the top of the culture reaction mechanism (1); A reaction vessel fixing mechanism (2) is arranged inside the culture reaction mechanism (1), and a reaction vessel body (210) is arranged on the top of the reaction vessel fixing mechanism (2); It is characterized in that Also includes: The culture reaction mechanism (1) comprises a culture workbench (101), wherein first support columns (102) are fixedly installed around the bottom of the culture workbench (101), and a support base plate (103) is fixedly installed at the bottom of the first support column (102); Wherein, support legs (104) are fixedly installed around the bottom of the support base plate (103), and a sealing groove (105) is fixedly installed on the inner side of the bottom of the culture workbench (101); A cylinder (106) is fixedly mounted on the bottom of the sealing groove (105), and the cylinder (106) is fixedly mounted on the top of the supporting bottom plate (103).

2. A cell culture reactor according to claim 1, characterized in that: A first fixed sleeve (107) is fixedly installed at the center position of the top of the support base plate (103), a rotating motor (108) is fixedly installed at the center position of the bottom of the support base plate (103), a meshing gear ring (109) is fixedly installed inside the first fixed sleeve (107), an output end of the rotating motor (108) is fixedly connected to a rotating main gear (110), an outer inner ring of the rotating main gear (110) is connected to a rotating driven gear (111), and the rotating driven gear (111) is meshedly connected to the meshing gear ring (109).

3. A cell culture reactor according to claim 2, characterized in that: The top of the rotating driven gear (111) is rotatably connected to a rotating disk (112), the top outer side of the rotating disk (112) is fixedly mounted with a vibration wave ring (113), the top of the rotating main gear (110) is fixedly mounted with a rotating connecting rod (114), the top of the rotating connecting rod (114) is fixedly connected with a supporting disk (115), a plurality of rotating sliding grooves (116) are provided inside the supporting disk (115), and an inclined sliding rod (117) is fixedly mounted inside the rotating sliding groove (116).

4. A cell culture reactor according to claim 3, characterized in that: The outer side of the tilted sliding rod (117) is slidably connected to a tilted sliding sleeve (118), one side of the tilted sliding sleeve (118) is fixedly connected to a tilted spring (119), the top of the tilted sliding sleeve (118) is rotatably connected to a tilted rotating rod (120), the top of the tilted rotating rod (120) is rotatably connected to a first spherical rotating support (121), a placement plate (122) is fixedly mounted on the top of the first spherical rotating support (121), and second spherical rotating supports (123) are fixedly mounted around the bottom of the placement plate (122).

5. A cell culture reactor according to claim 4, characterized in that: The bottom of the second spherical rotating support (123) is rotatably connected to a vibration connecting rod (124), the bottom of the vibration connecting rod (124) is fixedly connected to a vibration spring (125), a spring telescopic rod (126) is arranged inside the vibration spring (125), a movable roller (127) is fixedly installed at the bottom of the spring telescopic rod (126), and the second support column (128) is fixedly installed around the top of the culture workbench (101).

6. A cell culture reactor according to claim 5, characterized in that: A supporting top plate (129) is fixedly mounted on the top of the second supporting column (128); a lifting cylinder (130) is fixedly mounted at the center position of the bottom of the supporting top plate (129); an output end of the lifting cylinder (130) is fixedly connected to a lifting plate (131); a lifting sliding sleeve (132) is fixedly mounted around the lifting plate (131); the lifting sliding sleeve (132) is slidably connected to the second supporting column (128); a protective cover (133) is fixedly mounted on the bottom of the lifting plate (131); and a sealing ring (134) is fixedly mounted on the outer side of the bottom of the protective cover (133).

7. A cell culture reactor according to claim 1, characterized in that: The reaction vessel fixing mechanism (2) comprises a fixing base (201), a second fixing sleeve (202) is fixedly installed at the inner center position of the fixing base (201), a clamping rotating rod (203) is rotatably connected to the top of the fixing base (201), a clamping sliding sleeve (204) is rotatably connected to the top of the clamping rotating rod (203), a clamping sliding rod (205) is slidably connected to the inside of the clamping sliding sleeve (204), and a clamping spring (206) is fixedly connected to one side of the clamping sliding sleeve (204).

8. A cell culture reactor according to claim 7, characterized in that: A clamping limit block (207) is fixedly installed on the top of the clamping sliding sleeve (204), a deformable arc plate (208) is fixedly installed on the top of the clamping limit block (207), a clamping bracket (209) is fixedly installed on the outer side of the clamping sliding rod (205), the reaction vessel body (210) is arranged on the inner side of the deformable arc plate (208), a vertical slot (211) is opened at the inner center position of the second fixed sleeve (202), and limiting sliding grooves (212) are symmetrically installed on both sides of the second fixed sleeve (202) close to the vertical slot (211).

9. A cell culture reactor according to claim 8, characterized in that: The second fixed sleeve (202) is provided with a fixed rotating groove (213) at the bottom near the vertical slot (211) and the limiting sliding groove (212), and a locking spring (214) is symmetrically installed inside the fixed rotating groove (213), and an opening and closing block (215) is fixedly installed at the end of the locking spring (214), and a rotating plate (216) is rotatably connected to the top center position of the clamping spring (206), and a connecting rod (217) is fixedly connected to the bottom center position of the rotating plate (216), and fixed blocks (218) are symmetrically installed on both sides of the bottom of the connecting rod (217).

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