DNA fluorescence detection device

Abstract

The DNA fluorescence detection device comprises a machine body, a display screen and a control button which are electrically connected to the front face of the machine body, and a sample box inserted into the bottom of the front face of the machine body, the sample box can carry a sample to move into the machine body and detect the sample through the machine body, and the left side and the right side of the machine body are fixedly connected with connecting blocks; a shaft rod is movably connected into the connecting block through a bearing, supports are fixedly connected to the two sides of the surface of the shaft rod, and a bearing frame is fixedly connected to the sides, away from the shaft rod, of the supports. Through the combination of the machine body, the display screen, the control button, the sample box and other components, the fluorescence detection function of a DNA sample is achieved, meanwhile, the design of the connecting blocks, the shaft rods, the supports, the bearing frames and the like on the two sides of the machine body provides a structural basis for the subsequent cooling function, the phenomenon that the temperature of the sample rises is avoided, and the detection efficiency is improved. And the cooling fan can be utilized in the sample detection process, so that the machine body can be cooled.

Description

Technical Field

[0001] The invention relates to the technical field of DNA fluorescence detection, in particular to a DNA fluorescence detection device. Background Art

[0002] DNA fluorescence detection is a method that uses fluorescence technology to detect and analyze DNA. DNA fluorescence detection is based on the property that fluorescent substances can emit fluorescence after being irradiated with excitation light. In DNA detection, fluorescent dyes or fluorescently labeled probes are usually used to mark DNA molecules, and then the intensity, wavelength and other parameters of the fluorescence signal are measured to analyze the content, structure and sequence of DNA.

[0003] For example, the patent application number published on the China Patent Network is: 202222458212.7, and the patent name is: Fluorescence detection device and fluorescence detection system, including an excitation light source, a detector, an optical waveguide and a blocking structure, wherein the excitation light source is used to generate excitation light, and the excitation light is used to excite the object to be tested to emit fluorescence; the detector is located on one side of the excitation light source, and the detector is used to detect fluorescence; the optical waveguide is respectively connected to the excitation light source, the detector and the object to be tested, and the optical waveguide is used to transmit the excitation light emitted by the excitation light source to the object to be tested, and the optical waveguide is also used to transmit the fluorescence emitted by the object to be tested to the detector; the blocking structure is located between the detector and the excitation light source, and the blocking structure is used to block the detector from receiving the excitation light. The blocking structure can block the excitation light in the space from reaching the detector. By setting the optical waveguide, the volume of the fluorescence detection device is small, which ensures that the volume of the fluorescence detection device is small and the detection accuracy is high.

[0004] However, in the existing DNA fluorescence detection process, the test sample is directly excited by laser. During long-term detection, the sample will accumulate energy due to laser irradiation, causing the temperature to rise. The abnormally high temperature will affect the survival rate of the DNA inside the sample, so the sample needs to be taken out and cooled frequently, affecting the detection efficiency.

[0005] Therefore, it is necessary to design and modify the DNA fluorescence detection device. Summary of the invention

[0006] In order to solve the problems raised in the above-mentioned background technology, the purpose of the present invention is to provide a DNA fluorescence detection device, which has the advantage of rapid cooling of samples, and solves the problem that in the existing DNA fluorescence detection process, the detection sample is directly excited by laser. During long-term detection, the sample will accumulate energy due to laser irradiation, resulting in temperature increase. The abnormally high temperature will affect the survival rate of the DNA inside the sample, so the sample needs to be taken out and cooled frequently, affecting the detection efficiency.

[0007] To achieve the above-mentioned object, the present invention provides the following technical solutions: a DNA fluorescence detection device, comprising a body; A display screen and control buttons electrically connected to the front of the machine body; The sample box is plugged into the bottom of the front of the machine body; The sample box can carry the sample to the interior of the body and detect it through the body. The left and right sides of the body are fixedly connected with connecting blocks, and the interior of the connecting block is movably connected with an axle through a bearing. Both sides of the surface of the axle are fixedly connected with brackets, and the side of the bracket away from the axle is fixedly connected with a carrying frame. A cooling fan is installed inside the carrying frame. The cooling fan is located on both sides of the body and can quickly dissipate the heat of the body. The bracket can cool the sample stored inside the sample box when carrying the carrying frame to swing to the front side of the body.

[0008] As a preferred embodiment of the present invention, a movable block is fixedly connected to the top of the body, and a transmission rod is movably connected to the inside of the movable block through a bearing, both ends of the transmission rod and the top of the shaft rod are fixedly connected to bevel gears, the bevel gears are meshed with each other, and a handle is fixedly connected to the surface of the transmission rod.

[0009] As a preferred embodiment of the present invention, a protective cover is fixedly connected to the outer side of the movable block, and the protective cover is wrapped around the surface of the bevel gear.

[0010] As a preferred embodiment of the present invention, a shielding frame is fixedly connected to the back of the carrying frame, and the shielding frame can symmetrically contact each other after following the swing of the carrying frame. After the swing, the shielding frame can be located in front of the display screen and the control button and shield the two.

[0011] As a preferred embodiment of the present invention, the left and right sides of the body are fixedly connected with elastic buckles, and the elastic buckles extend from the side away from the body to the front of the sample box. The inner side of the supporting frame is fixedly connected with a pressure plate, and the inner side of the pressure plate can contact with the elastic buckles. When the pressure plate follows the supporting frame to swing to both sides of the body, it can squeeze the elastic buckles to deform them.

[0012] As a preferred embodiment of the present invention, a sleeve plate is provided on the front side of the bottom of the body, a pushing rack is fixedly connected to the top of the sleeve plate, the pushing rack extends from the side of the sleeve plate to the inside of the body and is located at the bottom of the sample box, and the pushing rack can carry the sample box and move it inside the body during horizontal movement.

[0013] As a preferred embodiment of the present invention, the bottom end of the shaft is fixedly connected to a wheel disc, and the bottom of the wheel disc is fixedly connected to a slide rod, and the slide rod extends to the inside of the sleeve plate from the side away from the wheel disc, and the sleeve plate is slidably connected to the slide rod. During the rotation of the shaft, the slide rod can be driven to squeeze the sleeve plate so that it carries the pushing frame to move horizontally.

[0014] As a preferred embodiment of the present invention, heat dissipation grooves are provided on both sides of the body, and the heat dissipation fan can cover the heat dissipation grooves and cool the inside of the body through the heat dissipation grooves when the heat dissipation fan follows the bearing frame to swing to the outside of the body.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention realizes the fluorescence detection function of DNA samples through the combination of components such as the body, display screen, control buttons and sample box. At the same time, the connection blocks, shaft rods, brackets and bearing frames on both sides of the body provide a structural basis for the subsequent cooling function to avoid the phenomenon of temperature increase of the sample. In addition, the heat dissipation fan can be used during the sample detection process to cool the body.

[0016] 2. The present invention realizes the transmission control of the shaft and the bracket through the design of the bracket, transmission rod, bevel gear and handle on the top of the body, so that the heat dissipation fan in the supporting frame can swing to the front side of the body to cool the sample, which not only improves the flexibility of the device, but also ensures that the sample can be effectively cooled before being taken out.

[0017] 3. The present invention effectively wraps the helical gear through the protective cover design on the outside of the bracket, preventing accidental damage that may be caused by the gear during rotation, and improving the safety of the device.

[0018] 4. The present invention adopts the shielding frame design on the back of the carrying frame, which can symmetrically contact each other after the carrying frame swings, shielding the display screen and control buttons, preventing misoperation of these components during the cooling process, and improving the stability and reliability of the device.

[0019] 5. The present invention adopts the design of elastic buckles on both sides of the body and pressure plates on the inner side of the carrying frame. When the carrying frame swings to both sides of the body, the pressure plates can squeeze the elastic buckles to deform them, thereby fixing the sample box and preventing the samples from moving or falling during the detection process, thereby ensuring the accuracy of the detection.

[0020] 6. The present invention adopts the design of the sleeve plate and the push rack on the front side of the bottom of the body. During the rotation of the shaft rod, the sleeve plate can be squeezed by the sliding rod to cause the push rack to move horizontally, thereby carrying the sample box to be inserted and moved inside the body, which not only simplifies the process of placing and removing samples, but also improves the degree of automation of the device.

[0021] 7. The present invention provides a power source for the horizontal displacement of the push rack through the design of the wheel disc and the slide rod at the bottom end of the shaft rod. Through the cooperation of the wheel disc and the slide rod, the push rack is precisely controlled to ensure the stable movement of the sample box inside the body.

[0022] 8. The present invention adopts the design of the heat dissipation grooves on both sides of the body and the heat dissipation fan in the bearing frame, so that when the heat dissipation fan swings to the outside of the body, the inside of the body can be cooled through the heat dissipation grooves, which not only improves the heat dissipation efficiency of the device, but also prolongs the service life of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the structure of the present invention; Figure 2 It is a schematic diagram of the main structure of the present invention; Figure 3 It is a bottom view structural schematic diagram of the present invention; Figure 4 It is a schematic diagram of the extended structure of the present invention; Figure 5 This is a schematic diagram of the elastic buckle structure of the present invention; Figure 6 For the present invention Figure 3 Enlarged structural diagram at A in the middle.

[0024] In the figure: 1. body; 2. display screen; 3. control button; 4. sample box; 5. connecting block; 6. shaft rod; 7. bracket; 8. bearing frame; 9. cooling fan; 10. movable block; 11. transmission rod; 12. bevel gear; 13. handle; 14. protective cover; 15. shielding frame; 16. elastic buckle; 17. pressure plate; 18. sleeve plate; 19. pushing rack; 20. wheel; 21. slide rod; 22. heat sink. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] like Figures 1 to 6 As shown, a DNA fluorescence detection device provided by the present invention includes a body 1; A display screen 2 and a control button 3 electrically connected to the front of the body 1; A sample box 4 inserted into the bottom of the front side of the body 1; The sample box 4 can carry the sample to the interior of the body 1 and detect it through the body 1. The left and right sides of the body 1 are fixedly connected with connecting blocks 5. The interior of the connecting block 5 is movably connected with an axle rod 6 through a bearing. Both sides of the surface of the axle rod 6 are fixedly connected with brackets 7. The side of the bracket 7 away from the axle rod 6 is fixedly connected. A cooling fan 9 is installed inside the supporting frame 8. The cooling fan 9 is located on both sides of the body 1 and can quickly dissipate heat for the body 1. The bracket 7 can cool the sample stored in the sample box 4 when carrying the supporting frame 8 and swinging to the front side of the body 1.

[0027] refer to Figure 3 A movable block 10 is fixedly connected to the top of the body 1, and a transmission rod 11 is movably connected to the inside of the movable block 10 through a bearing. Both ends of the transmission rod 11 and the top of the shaft 6 are fixedly connected to bevel gears 12, and the bevel gears 12 are meshed with each other. A handle 13 is fixedly connected to the surface of the transmission rod 11.

[0028] As a technical optimization solution of the present invention, through the design of the bracket 7, transmission rod 11, bevel gear 12 and handle 13 on the top of the body 1, transmission control of the shaft 6 and the bracket 7 is realized, so that the cooling fan 9 in the supporting frame 8 can swing to the front side of the body 1 to cool the sample, which not only improves the flexibility of the device, but also ensures that the sample can be effectively cooled before being taken out.

[0029] refer to Figure 2 A protective cover 14 is fixedly connected to the outer side of the movable block 10 , and the protective cover 14 wraps around the surface of the bevel gear 12 .

[0030] As a technical optimization solution of the present invention, the protective cover 14 is designed on the outside of the bracket 7 to effectively wrap the bevel gear 12, thereby preventing accidental damage that may be caused by the gear during rotation and improving the safety of the device.

[0031] refer to Figure 2 A shielding frame 15 is fixedly connected to the back of the carrying frame 8. The shielding frame 15 can symmetrically contact each other after following the swing of the carrying frame 8. After swinging, the shielding frame 15 can be located in front of the display screen 2 and the control button 3 and shield the two.

[0032] As a technical optimization solution of the present invention, the shielding frame 15 is designed on the back of the supporting frame 8 so that they can contact each other symmetrically after the supporting frame 8 swings, thereby shielding the display screen 2 and the control button 3, preventing misoperation of these components during the cooling process, and improving the stability and reliability of the device.

[0033] refer to Figure 2The left and right sides of the body 1 are fixedly connected with elastic buckles 16, and the elastic buckle 16 extends from the side away from the body 1 to the front of the sample box 4. The inner side of the carrying frame 8 is fixedly connected with a pressure plate 17, and the inner side of the pressure plate 17 can contact with the elastic buckle 16. When the pressure plate 17 follows the carrying frame 8 to swing to the two sides of the body 1, it can squeeze the elastic buckle 16 to deform it.

[0034] As a technical optimization solution of the present invention, through the design of the elastic buckles 16 on both sides of the body 1 and the pressure plates 17 on the inner side of the carrying frame 8, when the carrying frame 8 swings to both sides of the body 1, the elastic buckles 16 can be squeezed by the pressure plates 17 to deform them, thereby fixing the sample box 4, preventing the sample from moving or falling during the detection process, and ensuring the accuracy of the detection.

[0035] refer to Figure 3 A sleeve plate 18 is provided on the front side of the bottom of the body 1, and a push rack 19 is fixedly connected to the top of the sleeve plate 18. The push rack 19 extends to the interior of the body 1 away from the side of the sleeve plate 18 and is located at the bottom of the sample box 4. The push rack 19 can carry the sample box 4 and move it inside the body 1 during horizontal movement.

[0036] As a technical optimization solution of the present invention, through the design of the sleeve plate 18 and the pushing rack 19 on the front bottom side of the body 1, during the rotation of the shaft 6, the sleeve plate 18 can be squeezed by the sliding rod 21 to cause the pushing rack 19 to move horizontally, thereby carrying the sample box 4 to be inserted and moved inside the body 1, which not only simplifies the process of placing and removing samples, but also improves the degree of automation of the device.

[0037] refer to Figure 3 The bottom end of the shaft 6 is fixedly connected with a wheel disc 20, and the bottom of the wheel disc 20 is fixedly connected with a slide rod 21. The slide rod 21 extends to the inside of the sleeve 18 away from the side of the wheel disc 20. The sleeve 18 is slidably connected with the slide rod 21. During the rotation of the shaft 6, the slide rod 21 can be driven to squeeze the sleeve 18 so that it carries the pushing frame 19 to move horizontally.

[0038] As a technical optimization solution of the present invention, the design of the wheel disc 20 and the slide rod 21 at the bottom end of the shaft 6 provides a power source for the horizontal displacement of the pushing rack 19. Through the cooperation of the wheel disc 20 and the slide rod 21, precise control of the pushing rack 19 is achieved, ensuring the stable movement of the sample box 4 inside the body 1.

[0039] refer to Figure 3 Both sides of the body 1 are provided with heat dissipation grooves 22 , and when the heat dissipation fan 9 follows the bearing frame 8 to swing to the outside of the body 1 , it can cover the heat dissipation grooves 22 and cool the inside of the body 1 through the heat dissipation grooves 22 .

[0040] As a technical optimization solution of the present invention, through the design of the heat dissipation grooves 22 on both sides of the body 1 and the heat dissipation fan 9 in the supporting frame 8, when the heat dissipation fan 9 swings to the outside of the body 1, the inside of the body 1 can be cooled through the heat dissipation grooves 22, which not only improves the heat dissipation efficiency of the device, but also extends the service life of the device.

[0041] The working principle and use process of the present invention are as follows: first, the device is in the initial state, the display screen 2 and the control button 3 on the front of the body 1 are clearly visible, the sample box 4 is plugged into the bottom of the front of the body 1, waiting for the sample to be put in, the left and right sides of the body 1 are movably connected to the shaft 6 through the connecting block 5 and the bearing, the shaft 6 is fixed with a bracket 7, and the bracket 7 is installed with a bearing frame 8 and a cooling fan 9. At this time, the cooling fan 9 is located on both sides of the body 1, and the bracket 7 is in the standby position, and the sample in the sample box 4 is not cooled. When the sample needs to be tested, the transmission rod 11 in the top movable block 10 of the body 1 is first operated, and the transmission rod Bevel gears 12 are fixed to both ends of 11 and the top of the shaft 6, and these bevel gears 12 are meshed with each other. By rotating the handle 13 on the transmission rod 11, the shaft 6 and the bracket 7 can be driven to rotate together. At this time, the bracket 7 will carry the carrying frame 8 and the cooling fan 9 to the front side of the body 1. At the same time, the shielding frame 15 on the back of the carrying frame 8 will also contact each other symmetrically to shield the display screen 2 and the control button 3 to prevent accidental touch during operation. During the swinging of the bracket 7, the cooling fan 9 in the carrying frame 8 begins to pre-cool the sample stored in the sample box 4 to reduce the temperature of the sample and prepare for subsequent fluorescence detection. When the sample is placed, the shaft 6 rotates in the opposite direction, and the wheel disc 20 and the slide bar 21 at the bottom thereof begin to function. The wheel disc 20 drives the slide bar 21 to slide inside the sleeve plate 18, and the sleeve plate 18 is slidably connected to the slide bar 21. As the shaft 6 rotates and resets, the slide bar 21 squeezes the sleeve plate 18, causing it to carry the push rack 19 to move horizontally. The side of the push rack 19 away from the sleeve plate 18 extends to the inside of the body 1 and is located at the bottom of the sample box 4. Therefore, during the horizontal movement of the push rack 19, it will carry the sample box 4 to move in the body 1 until the sample box 4 reaches the detection position. At the same time, the pressure plate 17 on the inner side of the bracket 7 will also move horizontally. Swinging, when the pressure plate 17 follows the supporting frame 8 to swing to the two sides of the body 1, it will squeeze the elastic buckles 16 on both sides of the body 1 to deform and fix the sample box 4, ensuring the stability of the sample during the detection process. When the sample box 4 is plugged in, the detection system inside the body 1 starts to work and performs DNA fluorescence detection on the sample. During the detection process, if the body 1 generates heat, the cooling fan 9 will play a role again. When the supporting frame 8 swings to the outside of the body 1, the cooling fan 9 will block the heat dissipation slot 22 of the body 1 and cool the inside of the body 1 through the heat dissipation slot 22 to ensure the stability and accuracy of the detection process.

[0042] In summary, the DNA fluorescence detection device realizes the fluorescence detection function of the DNA sample through the combination of the body 1, the display screen 2, the control button 3 and the sample box 4. At the same time, the connection block 5, the shaft rod 6, the bracket 7 and the supporting frame 8 on both sides of the body 1 provide a structural basis for the subsequent cooling function to avoid the phenomenon of temperature increase of the sample, and the heat dissipation fan 9 can be used during the sample detection process to enable it to cool the body 1.

[0043] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A DNA fluorescence detection device, comprising a body (1); A display screen (2) and a control button (3) electrically connected to the front of the body (1); A sample box (4) plugged into the bottom of the front side of the body (1); Features: The sample box (4) can carry the sample to move to the inside of the body (1) and detect it through the body (1). The left and right sides of the body (1) are fixedly connected to connecting blocks (5). The inside of the connecting block (5) is movably connected to a shaft (6) through a bearing. Both sides of the surface of the shaft (6) are fixedly connected to brackets (7). The side of the bracket (7) away from the shaft (6) is fixedly connected to a bearing frame (8). A heat dissipation fan (9) is installed inside the bearing frame (8). The heat dissipation fan (9) is located on both sides of the body (1) and can quickly dissipate heat from the body (1). The bracket (7) can cool the sample stored inside the sample box (4) when carrying the bearing frame (8) and swinging to the front side of the body (1).

2. A DNA fluorescence detection device according to claim 1, characterized in that: A movable block (10) is fixedly connected to the top of the machine body (1), a transmission rod (11) is movably connected to the inside of the movable block (10) via a bearing, both ends of the transmission rod (11) and the top of the shaft rod (6) are fixedly connected to bevel gears (12), the bevel gears (12) are meshed with each other, and a handle (13) is fixedly connected to the surface of the transmission rod (11).

3. A DNA fluorescence detection device according to claim 2, characterized in that: A protective cover (14) is fixedly connected to the outer side of the movable block (10), and the protective cover (14) wraps around the surface of the bevel gear (12).

4. A DNA fluorescence detection device according to claim 1, characterized in that: A shielding frame (15) is fixedly connected to the back of the carrying frame (8), and the shielding frames (15) can symmetrically contact each other after following the swing of the carrying frame (8). After the swing, the shielding frame (15) can be located in front of the display screen (2) and the control button (3) to shield the two.

5. A DNA fluorescence detection device according to claim 1, characterized in that: The left and right sides of the body (1) are fixedly connected with elastic buckles (16), and the elastic buckle (16) extends from the side away from the body (1) to the front of the sample box (4). The inner side of the supporting frame (8) is fixedly connected with a pressure plate (17), and the inner side of the pressure plate (17) can contact the elastic buckle (16). When the pressure plate (17) follows the supporting frame (8) to swing to the two sides of the body (1), it can squeeze the elastic buckle (16) to deform it.

6. A DNA fluorescence detection device according to claim 1, characterized in that: A sleeve plate (18) is arranged at the front side of the bottom of the machine body (1), and a push rack (19) is fixedly connected to the top of the sleeve plate (18). The push rack (19) extends from a side of the sleeve plate (18) to the inside of the machine body (1) and is located at the bottom of the sample box (4). The push rack (19) can carry the sample box (4) to be inserted and moved inside the machine body (1) during horizontal movement.

7. A DNA fluorescence detection device according to claim 6, characterized in that: The bottom end of the shaft (6) is fixedly connected to a wheel disc (20), and the bottom of the wheel disc (20) is fixedly connected to a slide bar (21). The slide bar (21) extends to the inside of the sleeve plate (18) from a side away from the wheel disc (20). The sleeve plate (18) and the slide bar (21) are slidably connected. During the rotation of the shaft (6), the slide bar (21) can be driven to squeeze the sleeve plate (18) so that the sleeve plate (18) and the push frame (19) can be horizontally displaced.

8. A DNA fluorescence detection device according to claim 1, characterized in that: Both sides of the machine body (1) are provided with heat dissipation grooves (22), and when the heat dissipation fan (9) follows the support frame (8) to swing to the outside of the machine body (1), it can shield the heat dissipation grooves (22) and cool the interior of the machine body (1) through the heat dissipation grooves (22).

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