Process for producing functional liquid fertilizer from lincomycin waste liquid

Abstract

The invention belongs to the field of liquid fertilizers, and particularly relates to a method for preparing a liquid fertilizer from lincomycin waste liquid to improve the yield of food crops, which comprises the following steps: step 1, fermentation treatment: obtaining a lincomycin dry product; step 2, fermenting: fermenting for 36-110 hours, and purifying lincomycin from fermentation liquor by using an extraction reagent formed by mixing methanol and ethyl acetate after fermentation is completed; 3, synthesizing: weighing the potassium fulvate and the basic fertilizer in proportion, adding the potassium fulvate and the basic fertilizer into part of water, and mixing at a high speed to obtain 60-90wt% of saturated mother liquor; the modified lauric acid, the modified palmitic acid and the lincomycin are weighed according to the proportion and evenly dispersed in the remaining water to form a 10-40 wt% solid-liquid mixture, and then the solid-liquid mixture is evenly ground through an ultramicro grinding machine; uniformly mixing and dispersing the saturated mother liquor and the solid-liquid mixture in the step S2 to obtain the functional liquid fertilizer produced from the lincomycin waste liquid. The liquid fertilizer prepared from the lincomycin waste liquid can effectively improve the yield of crops.

Description

Technical Field

[0001] The invention belongs to the field of functional liquid fertilizers, in particular to a process for producing functional liquid fertilizers from lincomycin waste liquid. Background Art

[0002] Liquid compound fertilizer (Fluid Fertilizer), also known as fluid compound fertilizer, commonly known as liquid fertilizer, is a liquid product containing two or more nutrients required by crops. These nutrients are dissolved in water as solutes to form a solution or suspended in water with the help of a suspending agent to form a suspended paste. Liquid fertilizers have been developed to date and there are many varieties, which can be roughly divided into two categories: liquid nitrogen fertilizer and liquid compound fertilizer. Among them, the effective substances of liquid nitrogen fertilizer include ammonium, nitrate and amide nitrogen, such as liquid ammonia, ammonia water, and nitrogen solution. Liquid compound fertilizer contains all the nutrients required for plant growth, such as nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and trace elements, and can also be added with organic substances soluble in water (such as humic acid, amino acids, plant growth regulators, etc.). Liquid compound fertilizer can be designed according to the nutritional requirements of crop growth. The fertilizer formula can be adjusted at any time according to the different growth of crops. Scientific formulas can significantly improve fertilizer utilization. The fertilizer efficiency of liquid fertilizers is usually more than 30% higher than that of conventional compound fertilizers.

[0003] Lincomycin, also known as super tylosin, has a chemical name of 3-O-acetyl-4”-O-isovaleryl tylosin, and is a special antibiotic for livestock and poultry. Lincomycin is a new type of macrolide antibiotic obtained by biotransformation of tylosin by Streptomyces thermotolerans, acetylated at the 3-position and isovalerylated at the 4” position. Due to the change in its chemical structure, it has obvious advantages over tylosin in terms of antibacterial spectrum, antibacterial activity, drug resistance, chemical stability, oral absorption and blood drug concentration.

[0004] In the prior art, liquid fertilizer has the following problems: Unreasonable formula: When determining the liquid fertilizer formula, many manufacturers do not design it according to the fertilizer requirements of crops at different stages, resulting in the proportion of nutrient elements in the fertilizer not meeting the actual needs of the crops.

[0005] Poor stability: Due to the high technical content of liquid fertilizers, many products will produce crystallization, flocculation, stratification, flatulence and other adverse phenomena after being stored for a long time, which seriously affects the stability and use effect of the fertilizer. Summary of the invention

[0006] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0007] The technical solution adopted by the present invention to solve the technical problem is: a process for producing functional liquid fertilizer from lincomycin waste liquid of the present invention comprises the following steps: Step 1: fermentation treatment: adding 0.1-1% w / v of adsorbent to lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: S1, weigh potassium humate and basic fertilizer in proportion, add them to part of the water, and mix them at high speed to obtain 60-90wt% saturated mother liquor; S2, weigh modified lauric acid, modified palmitic acid, and lincomycin in proportion, evenly disperse them in the remaining water to form a 10-40wt% solid-liquid mixture, and then grind it evenly with an ultrafine grinder; S3, mix and disperse the saturated mother liquor in step S1 and the solid-liquid mixture in step S2 to obtain lincomycin waste liquid for producing functional liquid fertilizer.

[0008] As a further technical solution of the present invention: the acid water is one of hydrochloric acid or sulfuric acid with a content of 5%-10%, and the amount of acid water is calculated based on 10g / L lactic streptococcus; the pore size of the microfiltration membrane is 0.45um.

[0009] As a further technical solution of the present invention: 2.75-3.2 parts of modified lauric acid, 1.85-2.1 parts of modified palmitic acid, 8.6-10.2 parts of potassium humate, 57.5-64 parts of water, 25.6-29.8 parts of basic fertilizer, and 1.36-1.86 parts of lincomycin.

[0010] As a further technical solution of the present invention: the preparation process of modified lauric acid comprises the following steps: Dispersing chitosan in deionized water, adding glacial acetic acid and stirring to obtain a chitosan solution; Dissolve 2,3-epoxypropyltrimethylammonium chloride in deionized water, add the 2,3-epoxypropyltrimethylammonium chloride solution dropwise to the chitosan solution for reaction, put the reaction solution into a dialysis bag, dialyze in deionized water, and freeze-dry to obtain quaternized chitosan; The quaternized chitosan was dissolved in deionized water, carbodiimide hydrochloride, N-hydroxysuccinimide and lauric acid were dissolved in methanol for activation, and the QCS solution was added dropwise to the activation solution for reaction at room temperature; The reaction solution was placed in a dialysis bag, dialyzed in deionized water, and freeze-dried to obtain modified lauric acid.

[0011] As a further technical solution of the present invention: the mass ratio of chitosan: deionized water, glacial acetic acid, and 2,3-epoxypropyltrimethylammonium chloride is 10-20:1000-2000:1.2-1.6:15-25.

[0012] As a further technical solution of the present invention: the mass ratio of quaternized chitosan, carbodiimide hydrochloride, N-hydroxysuccinimide and lauric acid, methanol, and QCS solution is 10-15:10-50:7-25:300-400:1-5.

[0013] As a further technical solution of the present invention: the preparation process of modified palmitic acid comprises the following steps: Weigh 100g of cassava starch, add 10% palmitic acid, 5% vinyl acetate, 1.5% divinylbenzene and 12% composite catalyst based on the mass of starch, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 130-140°C for 3.5 hours to obtain palmitate-acetylated-cross-linked composite modified starch; add 2.5% sodium polyacrylate based on the mass of starch and 2% coupling agent, 3-(2,3-epoxypropoxy)propyltrimethoxysilane, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 100-110°C for 3 hours to obtain palmitate-acetylated-cross-linked-coupled quadruple modified starch, that is, modified palmitic acid.

[0014] As a further technical solution of the present invention: the composite catalyst is a mixture of ferrous sulfate, sodium persulfate and sodium silicate in a mass ratio of 1:3:20, and the amount used is 5-20% of the mass of cassava starch.

[0015] As a further technical solution of the present invention: the coupling agent is one of 3-triethoxysilyl propylamine, 3-(2,3-epoxypropyloxy)propyltrimethoxysilane and 3-(trimethoxymethylsilyl)propyl methacrylate, and the amount used is 0.2-3% of the mass of cassava starch.

[0016] The beneficial effects of the present invention are as follows: The fermentation treatment of the present invention comprises the following steps: adding 0.1-1% w / v of an adsorbent to lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by using a microfiltration water membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; fermentation: firstly performing fed-batch fermentation, and then converting to repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after continuing the fermentation for a period of time, glucose is added to the fermentation liquid; after continuing the fermentation for a period of time, entering repeated fed-batch fermentation. stage, draining a part of the fermentation liquid, adding fermentation medium and adding isobutanol to the fermentation liquid, and after a period of time, adding glucose to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, purifying lincomycin from the fermentation liquid with an extraction reagent mixed with methanol and ethyl acetate; synthesis: S1, weighing potassium humate and basic fertilizer in proportion, adding them to part of the water, and mixing them at high speed to obtain a saturated mother liquor of 60-90wt%; S2, weighing modified lauric acid, modified palmitic acid, and lincomycin in proportion, uniformly dispersing them in the remaining water to form a solid-liquid mixture of 10-40wt%, and then grinding them evenly with an ultrafine grinder; S3, mixing and dispersing the saturated mother liquor in step S1 and the solid-liquid mixture in step S2 to obtain a functional liquid fertilizer produced by lincomycin waste liquid; The functional liquid fertilizer produced by the lincomycin waste liquid of the present invention can effectively improve the growth of crops. DETAILED DESCRIPTION

[0017] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0018] Embodiment 1 The process for producing functional liquid fertilizer from lincomycin waste liquid according to an embodiment of the present invention comprises the following steps: Step 1: fermentation treatment: adding 0.1% w / v of an adsorbent to the lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Wherein, the acid water is one of hydrochloric acid or sulfuric acid with a content of 5%, and the amount of acid water is calculated based on 10g / L lactic acid streptococcus; the pore size of the microfiltration membrane is 0.45um; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: S1, weigh potassium humate and basic fertilizer in proportion, add them to part of the water, and mix them at high speed to obtain a 90wt% saturated mother liquor; S2, weigh modified lauric acid, modified palmitic acid, and lincomycin in proportion, evenly disperse them in the remaining water to form a 40wt% solid-liquid mixture, and then grind them evenly with an ultrafine grinder; S3, mix and disperse the saturated mother liquor in step S1 and the solid-liquid mixture in step S2 to obtain a functional liquid fertilizer for producing lincomycin waste liquid; Among them, 3.2 parts of modified lauric acid, 2.1 parts of modified palmitic acid, 10.2 parts of potassium humate, 64 parts of water, 29.8 parts of basic fertilizer, and 1.86 parts of lincomycin; The preparation process of modified lauric acid comprises the following steps: Dispersing chitosan in deionized water, adding glacial acetic acid and stirring to obtain a chitosan solution; Dissolve 2,3-epoxypropyltrimethylammonium chloride in deionized water, add the 2,3-epoxypropyltrimethylammonium chloride solution dropwise to the chitosan solution for reaction, put the reaction solution into a dialysis bag, dialyze in deionized water, and freeze-dry to obtain quaternized chitosan; The quaternized chitosan was dissolved in deionized water, carbodiimide hydrochloride, N-hydroxysuccinimide and lauric acid were dissolved in methanol for activation, and the QCS solution was added dropwise to the activation solution for reaction at room temperature; The reaction solution was placed in a dialysis bag, dialyzed in deionized water, and freeze-dried to obtain modified lauric acid; The mass ratio of chitosan: deionized water, glacial acetic acid, and 2,3-epoxypropyltrimethylammonium chloride is 20:2000:1.6:25; The mass ratio of quaternized chitosan, carbodiimide hydrochloride, N-hydroxysuccinimide and lauric acid, methanol, and QCS solution was 15:50:25:400:5; The preparation process of modified palmitic acid comprises the following steps: Weigh 100g of cassava starch, add 10% palmitic acid, 5% vinyl acetate, 1.5% divinylbenzene and 12% composite catalyst based on the mass of starch, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 130-140°C for 3.5 hours to obtain palmitate-acetylated-crosslinked composite modified starch; add 2.5% sodium polyacrylate and 2% coupling agent, 3-(2,3-epoxypropoxy)propyltrimethoxysilane based on the mass of starch, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 100-110°C for 3 hours to obtain palmitate-acetylated-crosslinked-coupled quadruple modified starch, that is, modified palmitic acid; The composite catalyst is a mixture of ferrous sulfate, sodium persulfate and sodium silicate in a mass ratio of 1:3:20, and the amount used is 5% of the mass of cassava starch; The coupling agent is one of 3-triethoxysilyl propylamine, 3-(2,3-epoxypropyloxy)propyltrimethoxysilane and 3-(trimethoxymethylsilyl)propyl methacrylate, and the amount used is 0.2% of the mass of cassava starch.

[0019] Embodiment 2 The process for producing functional liquid fertilizer from lincomycin waste liquid according to an embodiment of the present invention comprises the following steps: Step 1: fermentation treatment: adding 0.5% w / v of an adsorbent to the lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Wherein, the acid water is one of hydrochloric acid or sulfuric acid with a content of 7%, and the amount of acid water is calculated based on 10g / L lactic acid streptococcus; the pore size of the microfiltration membrane is 0.45um; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: S1, weigh potassium humate and basic fertilizer in proportion, add them to part of the water, and mix them at high speed to obtain 80wt% saturated mother liquor; S2, weigh modified lauric acid, modified palmitic acid, and lincomycin in proportion, evenly disperse them in the remaining water to form a 30wt% solid-liquid mixture, and then grind them evenly with an ultrafine grinder; S3, mix and disperse the saturated mother liquor in step S1 and the solid-liquid mixture in step S2 to obtain lincomycin waste liquid for producing functional liquid fertilizer; Among them, 2.92 parts of modified lauric acid, 1.99 parts of modified palmitic acid, 9.2 parts of potassium humate, 62 parts of water, 27.8 parts of basic fertilizer, and 1.56 parts of lincomycin; The preparation process of modified lauric acid comprises the following steps: Dispersing chitosan in deionized water, adding glacial acetic acid and stirring to obtain a chitosan solution; Dissolve 2,3-epoxypropyltrimethylammonium chloride in deionized water, add the 2,3-epoxypropyltrimethylammonium chloride solution dropwise to the chitosan solution for reaction, put the reaction solution into a dialysis bag, dialyze in deionized water, and freeze-dry to obtain quaternized chitosan; The quaternized chitosan was dissolved in deionized water, carbodiimide hydrochloride, N-hydroxysuccinimide and lauric acid were dissolved in methanol for activation, and the QCS solution was added dropwise to the activation solution for reaction at room temperature; The reaction solution was placed in a dialysis bag, dialyzed in deionized water, and freeze-dried to obtain modified lauric acid; The mass ratio of chitosan: deionized water, glacial acetic acid, and 2,3-epoxypropyltrimethylammonium chloride is 15:1500:1.4:20; The mass ratio of quaternized chitosan, carbodiimide hydrochloride, N-hydroxysuccinimide and lauric acid, methanol, and QCS solution was 13:30:15:350:3; The preparation process of modified palmitic acid comprises the following steps: Weigh 100g of cassava starch, add 10% palmitic acid, 5% vinyl acetate, 1.5% divinylbenzene and 12% composite catalyst based on the mass of starch, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 130-140°C for 3.5 hours to obtain palmitate-acetylated-crosslinked composite modified starch; add 2.5% sodium polyacrylate and 2% coupling agent, 3-(2,3-epoxypropoxy)propyltrimethoxysilane based on the mass of starch, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 100-110°C for 3 hours to obtain palmitate-acetylated-crosslinked-coupled quadruple modified starch, that is, modified palmitic acid; The composite catalyst is a mixture of ferrous sulfate, sodium persulfate and sodium silicate in a mass ratio of 1:3:20, and the amount used is 10% of the mass of cassava starch; The coupling agent is one of 3-triethoxysilyl propylamine, 3-(2,3-epoxypropyloxy)propyltrimethoxysilane and 3-(trimethoxymethylsilyl)propyl methacrylate, and the amount used is 2% of the mass of cassava starch.

[0020] Embodiment 3 The process for producing functional liquid fertilizer from lincomycin waste liquid according to an embodiment of the present invention comprises the following steps: Step 1: fermentation treatment: adding 1% w / v of adsorbent to lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Wherein, the acid water is one of hydrochloric acid or sulfuric acid with a content of 10%, and the amount of acid water is calculated based on 10g / L lactic acid streptococcus; the pore size of the microfiltration membrane is 0.45um; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: S1, weigh potassium humate and basic fertilizer in proportion, add them to part of the water, and mix them at high speed to obtain a 90wt% saturated mother liquor; S2, weigh modified lauric acid, modified palmitic acid, and lincomycin in proportion, evenly disperse them in the remaining water to form a 40wt% solid-liquid mixture, and then grind them evenly with an ultrafine grinder; S3, mix and disperse the saturated mother liquor in step S1 and the solid-liquid mixture in step S2 to obtain a functional liquid fertilizer for producing lincomycin waste liquid; Among them, 3.2 parts of modified lauric acid, 2.1 parts of modified palmitic acid, 10.2 parts of potassium humate, 64 parts of water, 29.8 parts of basic fertilizer, and 1.86 parts of lincomycin; The preparation process of modified lauric acid comprises the following steps: Dispersing chitosan in deionized water, adding glacial acetic acid and stirring to obtain a chitosan solution; Dissolve 2,3-epoxypropyltrimethylammonium chloride in deionized water, add the 2,3-epoxypropyltrimethylammonium chloride solution dropwise to the chitosan solution for reaction, put the reaction solution into a dialysis bag, dialyze in deionized water, and freeze-dry to obtain quaternized chitosan; The quaternized chitosan was dissolved in deionized water, carbodiimide hydrochloride, N-hydroxysuccinimide and lauric acid were dissolved in methanol for activation, and the QCS solution was added dropwise to the activation solution for reaction at room temperature; The reaction solution was placed in a dialysis bag, dialyzed in deionized water, and freeze-dried to obtain modified lauric acid; The mass ratio of chitosan: deionized water, glacial acetic acid, and 2,3-epoxypropyltrimethylammonium chloride is 20:2000:1.6:25; The mass ratio of quaternized chitosan, carbodiimide hydrochloride, N-hydroxysuccinimide and lauric acid, methanol, and QCS solution was 15:50:25:400:5; The preparation process of modified palmitic acid comprises the following steps: Weigh 100g of cassava starch, add 10% palmitic acid, 5% vinyl acetate, 1.5% divinylbenzene and 12% composite catalyst based on the mass of starch, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 130-140°C for 3.5 hours to obtain palmitate-acetylated-crosslinked composite modified starch; add 2.5% sodium polyacrylate and 2% coupling agent, 3-(2,3-epoxypropoxy)propyltrimethoxysilane based on the mass of starch, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 100-110°C for 3 hours to obtain palmitate-acetylated-crosslinked-coupled quadruple modified starch, that is, modified palmitic acid; The composite catalyst is a mixture of ferrous sulfate, sodium persulfate and sodium silicate in a mass ratio of 1:3:20, and the amount used is 20% of the mass of cassava starch; The coupling agent is one of 3-triethoxysilyl propylamine, 3-(2,3-epoxypropyloxy)propyltrimethoxysilane and 3-(trimethoxymethylsilyl)propyl methacrylate, and the amount used is 3% of the mass of cassava starch.

[0021] Comparative Example 1 Comparative Example 1 adopts the preparation process of a liquid fertilizer, comprising the following steps: Weigh potassium humate and basic fertilizer in proportion, add them to part of the water, and mix them at high speed to obtain a 60wt% saturated mother liquor; S2, weigh lauric acid, palmitic acid, and lincomycin in proportion, evenly disperse them in the remaining water to form a 10wt% solid-liquid mixture, and then grind them evenly with an ultrafine grinder; S3, mix and disperse the saturated mother liquor in step S1 and the solid-liquid mixture in step S2 to obtain a functional liquid fertilizer for producing lincomycin waste liquid; Among them, 2.75 parts of lauric acid, 1.85 parts of palmitic acid, 8.6 parts of potassium humate, 57.5 parts of water, and 25.6 parts of basic fertilizer.

[0022] Performance Testing: For the lincomycin finished liquid composite liquid fertilizer prepared in Examples 1-3 and Comparative Example 1, the field test was conducted with corn cultivation as the experimental object. Based on the plant height and root length of corn, the effects of different lincomycin finished liquid composite fertilizers on corn production were obtained, and Table 1 was obtained:

[0023] It can be seen from the above table that the functional liquid fertilizer produced by the lincomycin waste liquid of the present invention can effectively improve the growth of crops.

[0024] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A process for producing functional liquid fertilizer from lincomycin waste liquid, characterized in that: The following steps are involved: Step 1: fermentation treatment: adding 0.1-1% w / v of adsorbent to lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: S1, weigh potassium humate and basic fertilizer in proportion, add them to part of the water, and mix them at high speed to obtain 60-90wt% saturated mother liquor; S2, weigh modified lauric acid, modified palmitic acid, and lincomycin in proportion, evenly disperse them in the remaining water to form a 10-40wt% solid-liquid mixture, and then grind it evenly with an ultrafine grinder; S3, mix and disperse the saturated mother liquor in step S1 and the solid-liquid mixture in step S2 to obtain lincomycin waste liquid for producing functional liquid fertilizer.

2. A process for producing functional liquid fertilizer from lincomycin waste liquid according to claim 1, characterized in that: The acid water is one of hydrochloric acid or sulfuric acid with a content of 5%-10%, and the amount of acid water is calculated based on 10g / L lactic streptococcus; the pore size of the microfiltration membrane is 0.45um.

3. A process for producing functional liquid fertilizer from lincomycin waste liquid according to claim 2, characterized in that: 2.75-3.2 parts of modified lauric acid, 1.85-2.1 parts of modified palmitic acid, 8.6-10.2 parts of potassium humate, 57.5-64 parts of water, 25.6-29.8 parts of basic fertilizer, and 1.36-1.86 parts of lincomycin.

4. A process for producing functional liquid fertilizer from lincomycin waste liquid according to claim 3, characterized in that: The preparation process of modified lauric acid comprises the following steps: Dispersing chitosan in deionized water, adding glacial acetic acid and stirring to obtain a chitosan solution; Dissolve 2,3-epoxypropyltrimethylammonium chloride in deionized water, add the 2,3-epoxypropyltrimethylammonium chloride solution dropwise to the chitosan solution for reaction, put the reaction solution into a dialysis bag, dialyze in deionized water, and freeze-dry to obtain quaternized chitosan; The quaternized chitosan was dissolved in deionized water, carbodiimide hydrochloride, N-hydroxysuccinimide and lauric acid were dissolved in methanol for activation, and the QCS solution was added dropwise to the activation solution for reaction at room temperature; The reaction solution was placed in a dialysis bag, dialyzed in deionized water, and freeze-dried to obtain modified lauric acid.

5. A process for producing functional liquid fertilizer from lincomycin waste liquid according to claim 4, characterized in that the mass ratio of chitosan: deionized water, glacial acetic acid, and 2,3-epoxypropyltrimethylammonium chloride is 10-20:1000-2000:1.2-1.6:15-25.

6. A process for producing functional liquid fertilizer from lincomycin waste liquid according to claim 5, characterized in that: The mass ratio of quaternized chitosan, carbodiimide hydrochloride, N-hydroxysuccinimide, lauric acid, methanol and QCS solution is 10-15:10-50:7-25:300-400:1-5.

7. A process for producing functional liquid fertilizer from lincomycin waste liquid according to claim 6, characterized in that: The preparation process of modified palmitic acid comprises the following steps: Weigh 100g of cassava starch, add 10% palmitic acid, 5% vinyl acetate, 1.5% divinylbenzene and 12% composite catalyst based on the mass of starch, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 130-140°C for 3.5 hours to obtain palmitate-acetylated-cross-linked composite modified starch; add 2.5% sodium polyacrylate based on the mass of starch and 2% coupling agent, 3-(2,3-epoxypropoxy)propyltrimethoxysilane, mix well, and carry out semi-dry hot extrusion reaction at a temperature of 100-110°C for 3 hours to obtain palmitate-acetylated-cross-linked-coupled quadruple modified starch, that is, modified palmitic acid.

8. A process for producing functional liquid fertilizer from lincomycin waste liquid according to claim 8, characterized in that: The composite catalyst is a mixture of ferrous sulfate, sodium persulfate and sodium silicate in a mass ratio of 1:3:20, and the dosage is 5-20% of the mass of cassava starch.

9. A process for producing functional liquid fertilizer from lincomycin waste liquid according to claim 9, characterized in that: The coupling agent is one of 3-triethoxysilyl propylamine, 3-(2,3-epoxypropyloxy)propyltrimethoxysilane and 3-(trimethoxymethylsilyl)propyl methacrylate, and the dosage is 0.2-3% of the mass of cassava starch.

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