A new donkey-hide gelatin and its preparation process

Abstract

The present invention provides a preparation process of a new donkey-hide gelatin, which uses a traditional process combined with an acid method and an enzyme process to extract pig skin raw materials, and uses freeze drying or spray drying to obtain the final product. The method shortens the process steps and time, greatly improves the production efficiency, and at the same time, the yield of preparing the new donkey-hide gelatin is significantly improved compared with the original process, and the anti-aging and anti-oxidation effects of the new donkey-hide gelatin prepared by the method are significantly improved compared with the new donkey-hide gelatin prepared by the original process. The second purpose of the present invention is to provide an application of a new donkey-hide gelatin, which has good applications in anti-aging, anti-oxidation, and blood pressure reduction.

Description

Technical Field

[0001] The invention belongs to the technical field of drug preparation and relates to a new donkey-hide gelatin and a preparation process thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] In recent years, donkey-hide gelatin has been widely used in the field of health, and the downstream market demand for donkey-hide gelatin has gradually increased, resulting in some inferior products being sold as good ones and a mixture of good and bad products in the donkey-hide gelatin industry. Therefore, finding a substitute for donkey-hide gelatin is an important way to resolve the contradictions in this industry.

[0004] The history of pig skin gelatin for medicinal use began before the Ming Dynasty. Zhang Zhongjing of the Later Han Dynasty wrote in Treatise on Febrile Diseases·Shaoyin Chapter: "Shaoyin disease, diarrhea, sore throat, chest fullness and irritability, boil pig skin soup." The prescription in "Yi Zong Jin Jian" says: "Pig skin is the skin of leather." Li Shizhen's "Compendium of Materia Medica" says: "When making glue, from October to February or March, the skin of ox, buffalo and donkey is the best, and the skin of pig, horse, mule and camel is the second best." New donkey-hide gelatin (PHG) is a solid glue made from the skin of animal pigs (Sus scrofa domestica Brisson) through decoction and concentration. It is an exclusive product of Shandong Fujiao Group and is included in the "Standards of the Ministry of Health·Chinese Medicine Formula Preparations". Clinical verification has shown that new donkey-hide gelatin has the effects of nourishing yin deficiency, nourishing blood, and stopping bleeding. It can be used to treat weak body anemia, menstrual disorders, vomiting blood, epistaxis, thrombocytopenia and leukocytopenia, and is beneficial to improving the body's immune level.

[0005] The production method of new donkey-hide gelatin adopts the ancient method passed down from ancient times. Its process flow is: skin material → water soaking → boiling gelatin → filtering → concentration → adding material → forming → slicing → drying → sorting → testing → packaging → storage. At present, the preparation process of new donkey-hide gelatin is complicated and time-consuming, and the finished product needs to be crushed before consumption, which is inconvenient to eat. Summary of the invention

[0006] In view of the above problems, an object of the present invention is to provide a preparation process of a new donkey-hide gelatin, which uses a traditional process combined with an acid method and an enzyme method to extract the pig skin raw material, and adopts freeze-drying or spray-drying to obtain the final product. The use of this method shortens the process steps and time, greatly improves the production efficiency, and at the same time, the yield of the new donkey-hide gelatin is significantly improved compared with the original process, and the anti-aging and antioxidant effects of the new donkey-hide gelatin prepared by this method are significantly improved compared with the new donkey-hide gelatin prepared by the original process.

[0007] The second purpose of the present invention is to provide an application of a new donkey-hide gelatin, which has good application in lowering blood pressure.

[0008] A method for preparing a novel donkey-hide gelatin comprises the following steps:

[0009] Step 1: Select and weigh qualified pigskin, and cut it into cubes after scraping, washing, soaking and other processes;

[0010] Step 2: Add sodium carbonate to the pigskin obtained in step 1 at a mass ratio of 1:0.004-0.006, and then add water at a mass ratio of 1:0.3-0.5. Boil for 20-30 minutes, filter, and wash the pigskin with 1-2 times the amount of water.

[0011] Step 3: adding citric acid to the pig skin obtained in step 2 at a mass ratio of 1:0.03-0.04, and then adding water at a mass ratio of 1:0.8-1.2, boiling for 1-2 hours, filtering, and collecting the filtrate;

[0012] Step 4: adding water to the pigskin obtained in step 3 at a mass ratio of 1:0.8-1.2, adjusting the pH value to 7.0-8.0 with sodium carbonate, adding enzymes at a mass ratio of pigskin to collagenase and trypsin of 1:0.008-0.012:0.004-0.006, keeping warm at 30-40° C. for 2-3 hours, boiling for 2-3 hours, filtering, and collecting the filtrate;

[0013] Step 5: combining the secondary filtrates, performing liposuction treatment and drying treatment to obtain the new donkey-hide gelatin.

[0014] Preferably, the pretreatment in step 1 includes: selecting and weighing qualified pigskin, scraping off the grease and hair from the fresh skin, and putting it into a skin washing pool for washing and standby; directly putting the dried skin into a skin soaking pool, soaking for 4-5 days, changing the water 1-2 times a day, taking it out after soaking thoroughly, and removing the hair. The obtained pigskin is cut into 30-50 cm squares, and washed in a skin washing pool.

[0015] Preferably, in step 2, sodium carbonate is added to the pig skin in a mass ratio of 1:0.005 to sodium carbonate, and then water is added in a mass ratio of 1:0.4 to pig skin.

[0016] Preferably, in step three, citric acid is added in a mass ratio of pig skin to citric acid of 1:0.035, and then water is added in a ratio of 1:1 and boiled for 1.5 hours.

[0017] Preferably, in step 4, water is added to the pigskin in a mass ratio of 1:1, and the pH value is adjusted to 7.5 with sodium carbonate, and then enzymes are added in a mass ratio of pigskin to collagenase and trypsin of 1:0.01:0.005, and the mixture is kept warm at 55°C for 3 hours and then boiled for 3 hours.

[0018] Preferably, the combined filtrate in step five is added with activated carbon at a volume fraction of 1 to 2%, stirred for 10 to 20 minutes, allowed to stand for 2 to 3 hours, filtered, and the filtrate is dried by freeze drying or spray drying to obtain the new donkey-hide gelatin.

[0019] Beneficial Effects

[0020] The invention discloses a method for preparing a novel donkey-hide gelatin. The problem of impurities in donkey-hide gelatin is solved by adding sodium carbonate. The extraction rate of collagen is improved by combining an enzyme method with citric acid extraction, and the proportion of macromolecular collagen in the extract is reduced. Moreover, the extracted donkey-hide gelatin has good application in anti-aging, anti-oxidation and blood pressure reduction. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 SPB trend diagram of the control group, model group and experimental group;

[0022] Figure 2 DBP trend diagram of the control group, model group and experimental group;

[0023] Figure 3 This is the MBP trend chart of the control group, model group and experimental group. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below in conjunction with specific experimental examples.

[0025] Preparation process of original new donkey-hide gelatin:

[0026] S1, picking raw pig skin, selecting the pig skin as dry pig skin;

[0027] S2, immersing the dried pigskin obtained in step S1 in water for 3-5 days;

[0028] S3, scraping off the hair and attached grease from the pigskin obtained in step S2;

[0029] S4, cutting the pigskin obtained in step S3 into small pieces of 30-50 cm;

[0030] S5, washing the pigskin obtained in step S4 until the washing water is clear and free of sediment, and the salt content is reduced to below 20 mg / L;

[0031] S6, put the pigskin obtained in step S5 into a spheronizer, add water according to the mass ratio of pigskin to drinking water of 1:0.3-0.5. Pass clean steam, control the steam pressure at 0.12-0.15Mpa, blanch the skin for 20-30 minutes and then release the blanching water;

[0032] S7, adding water to the pigskin obtained in step S6 into a steam bulb at a mass ratio of 1:0.3-0.5 for pigskin and drinking water, first performing dynamic extraction for 0.5-1.5h, with the steam pressure controlled at 0.12-0.20Mpa, and then performing static extraction for 3-5h, with the steam pressure controlled at 0.12-0.15Mpa, rotating the steam bulb for 2-5min every hour, and filtering the extract into a storage tank after extraction;

[0033] S8, the wet pigskin obtained in step S7, mixed with water, according to the mass ratio of 1: 0.1-0.3, water was added, the amount of water was measured with a water meter, the water inlet valve was closed after the specified water volume was reached, the steam was started, clean steam was introduced, and the pressure indicated by the pressure gauge was controlled to be 0.12-0.15Mpa; the glue was extracted for 0.4-1h, filtered, and the glue filtrate extracted in step S7 was merged, and the extraction was completed and the residue was discharged;

[0034] S9, centrifuging the extract obtained in step S8 into a liquid storage tank through a three-legged centrifuge, and then centrifuging into a primary concentration pot through a disc separator;

[0035] S10, heating the glue juice put into the primary concentration pot in step S9 to boiling, carrying out preliminary concentration of this batch of glue liquid for 12 hours, and transferring it to the foaming pot;

[0036] S11, concentrating the glue obtained in step S10, adding water, and boiling for 6-8 hours to separate the fatty oil from the glue liquid, removing the fatty oil with a ladle and a foaming knife, and then heating and concentrating the glue, maintaining the pressure of the glue in the concentration pot at 0.05-0.10MPa, and removing the floating foam and impurities on the surface of the glue, extracting the oil 1-3 times and the foam 2-4 times according to the above method;

[0037] S12, dissolving rock sugar: add water twice the weight of rock sugar to heat the rock sugar, and set aside after all the rock sugar is dissolved. Dissolving tailings: add water to dissolve the tailings and set aside;

[0038] S13, all the glue liquid and the reserved tailing liquid obtained in steps S11 and S12 are successively transported to the same glue discharging pot through an electric diaphragm pump, and concentrated until the hanging beads can be picked up with a shovel, and soybean oil, dissolved rock sugar, and rice wine are added in sequence, and the concentration is continued until the moisture content of the glue reaches 26.0%-28.5%, and then the glue is discharged, and the glue liquid is transferred to a special stainless steel glue box and sent to the buffer room for buffering for 8 hours;

[0039] S14, transfer the glue box after buffering in step S13 to the primary setting room, control the temperature at -3℃-3℃, freeze for 8-12h to dig out the glue, send the dug out glue lump to the final setting room for freezing, control the temperature at 0℃-3℃, and condense to a suitable hardness. The hardness standard is that when the film is completely dry, two films hit each other, the sound is crisp and loud, and the film is broken into several small pieces when it falls to the ground. Use the broken film to pierce the glue core of another film. It is hard and cannot pierce a soft pit;

[0040] S15, cutting and drying the glue block obtained in step S14.

[0041] Experimental Example 1 Study on Blanching Process

[0042] Blanching can further remove impurities from the pig skin, mainly the grease on the fur, as well as some impurities that cannot be removed by soaking.

[0043]

[0044] The experimental results show that adding sodium carbonate during the blanching process can effectively remove impurities in the pig skin, especially the grease attached to the pig skin. At the same time, after blanching with sodium carbonate, the pig skin becomes soft, which is conducive to the next extraction process. Therefore, it was finally decided to add sodium carbonate during the blanching process.

[0045] Experimental Example 2: Extraction Process Study

[0046] The traditional extraction process of new donkey-hide gelatin uses water extraction and concentration. The extraction rate of collagen in pig skin is low, and the proportion of large-molecule collagen is large, which is not conducive to human absorption. The extraction process is improved to increase the extraction rate of collagen and reduce the proportion of large-molecule collagen in the extract.

[0047]

[0048]

[0049] Molecular weight determination of new donkey-hide gelatin:

[0050] With reference to the detection method for the proportion of collagen peptides with a relative molecular weight less than 10,000 in Appendix A of "GB31645-2018 National Food Safety Standard Collagen Peptide" (high performance size exclusion chromatography), the molecular weight distribution of the new donkey-hide gelatin prepared by process 10 in Example 2 was detected.

[0051]

[0052] Experimental Example 3 Anti-aging Study

[0053] Healthy AB zebrafish fertilized at 8hpf were used as animal models. There were 10 embryos in each group for the experiment, and 2 duplicate wells were set up at the same time. Each example was repeated three times. The above embryos were placed in a constant temperature incubator at 28.5°C for continuous treatment for 3 days (changing 1mL of new solution every other day). After the treatment, the zebrafish were washed with PBS buffer. Subsequently, the β-galactosidase in the above-mentioned groups of zebrafish was stained with a β-galactosidase staining kit, and the staining intensity reflected the β-galactosidase activity in the zebrafish. The coloring intensity was then calculated using Imagepro-plus software, and the data were statistically analyzed using GraphPad software to evaluate the anti-aging activity of the samples.

[0054] Serial number Sample group number Sample batch number Sample concentration (μg / ml) <![CDATA[β-galactosidase activity (×10 6 )]]> 01 Control group —— 0 9.059 02 Experimental Group 1 20220501 10 7.362 03 Experimental Group 2 20220502 10 6.026 04 Experimental Group 3 20220503 10 6.837 05 Experimental Group 4 20220504 10 6.527 06 Experimental Group 5 20220505 10 7.038 07 Experimental Group 6 20220506 10 7.935 08 Experimental Group 7 20220507 10 5.958 09 Experimental Group 8 20220508 10 6.721 10 Experimental Group 9 20220509 10 6.626 11 Experimental Group 10 20220510 10 5.365 12 Experimental Group 11 20220510 50 4.653 13 Experimental Group 12 20220510 100 3.928

[0055] All groups of zebrafish developed normally. The results of zebrafish β-galactosidase activity in experimental groups 1-10 were lower than those in the control group, but the decrease was most obvious in experimental group 10, indicating that the anti-aging effect was best when citric acid was used for primary extraction and then collagenase and trypsin were used for secondary extraction. Experimental groups 10-12 showed that within the concentration range of 10μg / ml to 100μg / ml, as the concentration of new donkey-hide gelatin increased, the β-galactosidase activity decreased more significantly, and the anti-aging effect showed a good increasing relationship.

[0056] Experimental Example 4 Antioxidant Study

[0057] The skin fluorescent zebrafish with 24h development were used as experimental animals, and 1mg / mL of pronase was used to remove the outer layer of the embryonic membrane. The embryos with the egg membrane removed were randomly assigned, and each group of experiments had 10 embryos. At the same time, 2 duplicate wells were set up, and the experimental results were averaged after each group of experiments were repeated three times. The above embryos were placed in a constant temperature incubator at 28.5℃, and after being treated with each experimental solution in Table 1 for 24h, the zebrafish were anesthetized with ethyl m-aminobenzoate methanesulfonate (MS222), and observed and photographed under a fluorescence microscope. The number of zebrafish skin fluorescent spots was counted using Imagepro-plus software, and the data were statistically analyzed using GraphPad software to evaluate the antioxidant activity of the samples.

[0058] Serial number Sample group number Sample batch number Metronidazole (mM) Sample concentration (μg / ml) Number of skin fluorescent spots 01 Control group 1 —— 0 0 140.8 02 Control group 2 —— 5 0 23.8 03 Control group 3 —— 5 100μg / ml Vc solution 106.9 04 Experimental Group 1 20220501 5 10 60.5 05 Experimental Group 2 20220502 5 10 66.4 06 Experimental Group 3 20220503 5 10 67.5 07 Experimental Group 4 20220504 5 10 62.1 08 Experimental Group 5 20220505 5 10 59.7 09 Experimental Group 6 20220506 5 10 53.7 10 Experimental Group 7 20220507 5 10 72.4 11 Experimental Group 8 20220508 5 10 68.9 12 Experimental Group 9 20220509 5 10 67.8 13 Experimental Group 10 20220510 5 10 76.3 14 Experimental Group 11 20220510 5 50 85.7 15 Experimental Group 12 20220510 5 100 90.5

[0059] Control group 1 was the blank control group, control group 2 was the metronidazole modeling group, and control group 3 was the positive control group.

[0060] All groups of zebrafish developed normally, but compared with control group 1, the number of zebrafish skin fluorescent spots in control group 2 decreased significantly, which clearly showed that under the influence of metronidazole, the zebrafish skin cells were oxidized; the number of zebrafish skin fluorescent spots in experimental groups 1-10 increased, but the increase in experimental group 10 was the largest, indicating that the antioxidant effect of the sample was the best when citric acid was used for primary extraction and then collagenase and trypsin were used for secondary extraction. Experimental groups 10-12 showed that within the concentration range of 10-100μg / ml, as the concentration of new donkey-hide gelatin increased, the number of zebrafish skin fluorescent spots also increased, and the antioxidant effect showed a good increasing relationship.

[0061] Experimental Example 5: Study on lowering blood pressure

[0062] 110 5-week-old male SPF Kunming mice (body weight (BW) (35±3) g) were maintained at (25±2)℃, relative humidity at (50±10)%, 12h / 12h light-dark cycle, free access to food and water. After 1 week of adaptive feeding, the mice were randomly divided into 11 groups (10 mice in each group): normal group, model group, and experimental groups. Except for the control group, hypertension was induced in the other groups (L-NAME, 40 mg / (kg·d), gavage for 6 weeks). The experimental groups were gavaged with the samples of each experimental case, and the normal group and model group were gavaged with an equal volume of normal saline for 6 weeks. The BW of each group of mice was recorded weekly during the period, and the systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean blood pressure (MBP) were measured using a non-invasive sphygmomanometer.

[0063] Serial number Sample group number Sample batch number Number of experimental animals Dosage (mg / kg) 01 Control group —— 10 0 02 Model Group —— 10 0 03 Experimental Group 1 20220501 10 25 04 Experimental Group 2 20220502 10 25 05 Experimental Group 3 20220503 10 25 06 Experimental Group 4 20220504 10 25 07 Experimental Group 5 20220505 10 25 08 Experimental Group 6 20220506 10 25 09 Experimental Group 7 20220507 10 25 10 Experimental Group 8 20220508 10 25 11 Experimental Group 9 20220509 10 25 12 Experimental Group 10 20220510 10 25 13 Experimental Group 11 20220510 10 50 14 Experimental Group 12 20220510 10 100

[0064] The above experimental results show that the SBP, DBP and MBP of mice in experimental groups 1-10 were lower than those in the control group, but the decrease was the largest in experimental group 10, indicating that the blood pressure lowering effect of the samples extracted with citric acid once and then extracted with collagenase and trypsin twice was the best. Experimental groups 10-12 showed that within the dosage range of 25-100 mg / kg, as the dosage of new donkey-hide gelatin increased, the blood pressure lowering effect showed a good increasing relationship.

[0065] Example 1

[0066] Step 1: Select and weigh qualified pigskin, and cut it into 30-50cm squares after scraping, washing, soaking and other processes;

[0067] Step 2: Add sodium carbonate to the pigskin obtained in step 1 at a mass ratio of 1:0.005, and then add water at a mass ratio of 1:0.4. Boil for 30 minutes, filter, and wash the pigskin with twice the amount of water.

[0068] Step 3: adding citric acid to the pig skin obtained in step 2 at a mass ratio of 1:0.03, and then adding water at a mass ratio of 1:0.8, boiling for 1 hour, filtering, and collecting the filtrate;

[0069] Step 4: adding water to the pigskin obtained in step 3 at a mass ratio of 1:1.2, adjusting the pH value to 7.0-8.0 with sodium carbonate, adding enzymes at a mass ratio of pigskin to collagenase and trypsin of 1:0.008:0.006, keeping warm at 30-40°C for 3 hours, boiling for 2 hours, filtering, and collecting the filtrate;

[0070] Step 5: Combine the secondary filtrates, add 2% by volume of activated carbon, stir for 20 minutes, let stand for 2 hours, filter, and freeze-dry the filtrate to obtain a new donkey-hide gelatin sample, batch number: 20220701.

[0071] Example 2

[0072] Step 1: Select and weigh qualified pigskin, and cut it into 30-50cm squares after scraping, washing, soaking and other processes;

[0073] Step 2: Add sodium carbonate to the pigskin obtained in step 1 at a mass ratio of 1:0.004, and then add water at a mass ratio of 1:0.3. Boil for 20 minutes, filter, and wash the pigskin with 1 times the amount of water.

[0074] Step 3: adding citric acid to the pig skin obtained in step 2 at a mass ratio of 1:0.03, and then adding water at a mass ratio of 1:1, boiling for 1 hour, filtering, and collecting the filtrate;

[0075] Step 4: adding water to the pigskin obtained in step 3 at a mass ratio of 1:0.8, adjusting the pH value to 7.0-8.0 with sodium carbonate, adding enzymes at a mass ratio of pigskin to collagenase and trypsin of 1:0.01:0.005, keeping warm at 30-40°C for 2 hours, boiling for 3 hours, filtering, and collecting the filtrate;

[0076] Step 5: Combine the secondary filtrates, add 1% by volume of activated carbon, stir for 10 minutes, let stand for 3 hours, filter, and freeze-dry the filtrate to obtain a new donkey-hide gelatin sample, batch number: 20220702.

[0077] Example 3

[0078] Step 1: Select and weigh qualified pigskin, and cut it into 30-50cm squares after scraping, washing, soaking and other processes;

[0079] Step 2: Add sodium carbonate to the pigskin obtained in step 1 at a mass ratio of 1:0.006, and then add water at a mass ratio of 1:0.5. Boil for 20 to 30 minutes, filter, and wash the pigskin with 1 to 2 times the amount of water.

[0080] Step 3: adding citric acid to the pigskin obtained in step 2 at a mass ratio of 1:0.04, and then adding water at a ratio of 1:1.2, boiling for 1 to 2 hours, filtering, and collecting the filtrate;

[0081] Step 4: adding water to the pigskin obtained in step 3 at a mass ratio of 1:1.0, adjusting the pH value to 7.0-8.0 with sodium carbonate, adding enzymes at a mass ratio of pigskin to collagenase and trypsin of 1:0.012:0.006, keeping warm at 30-40°C for 2 hours, boiling for 3 hours, filtering, and collecting the filtrate;

[0082] Step 5: Combine the secondary filtrates, add 1% by volume of activated carbon, stir for 10 minutes, let stand for 3 hours, filter, and spray dry the filtrate to obtain a new donkey-hide gelatin sample, batch number: 20220703.

Claims

1. A method for preparing a novel donkey-hide gelatin, characterized in that: The steps include: Step 1: Select and weigh qualified pig skin, scrape, wash, soak and cut into cubes; Step 2: adding sodium carbonate to the pigskin obtained in step 1 at a mass ratio of 1:0.004-0.006, and then adding water at a mass ratio of 1:0.3-0.5; boiling for 20-30 minutes, filtering, and washing the pigskin with 1-2 times the amount of water; Step 3: adding citric acid to the pigskin obtained in step 2 at a mass ratio of 1:0.03-0.04, and then adding water at a mass ratio of 1:0.8-1.2, boiling for 1-2 hours, filtering, and collecting the filtrate; Step 4: adding water to the pigskin obtained in step 3 at a mass ratio of 1:0.8-1.2, adjusting the pH value to 7.0-8.0 with sodium carbonate, adding enzymes at a mass ratio of pigskin to collagenase and trypsin of 1:0.008-0.012:0.004-0.006, keeping warm at 30-40° C. for 2-3 hours, boiling for 2-3 hours, filtering, and collecting the filtrate; Step 5: combining the secondary filtrates, performing liposuction treatment and drying treatment to obtain the new donkey-hide gelatin.

2. The method for preparing a novel donkey-hide gelatin according to claim 1, characterized in that: The pretreatment in step 1 includes: selecting and weighing qualified pig skin, scraping off the grease and hair from the fresh skin, and putting it into a skin washing pool for washing and standby use; directly putting the dry skin into a skin soaking pool, soaking for 4-5 days, changing the water 1-2 times a day, taking it out after soaking thoroughly, removing the hair, cutting the obtained pig skin into 30-50 cm squares, and washing it in a skin washing pool.

3. The method for preparing a novel donkey-hide gelatin according to claim 1, characterized in that: In step 2, sodium carbonate is added in a mass ratio of pig skin to sodium carbonate of 1:0.005, and then water is added in a mass ratio of pig skin to water of 1:0.

4.

4. The method for preparing a novel donkey-hide gelatin according to claim 1, characterized in that: In step 3, citric acid is added in a mass ratio of pig skin to citric acid of 1:0.035, and then water is added in a ratio of 1:1 and boiled for 1.5 hours.

5. The method for preparing a novel donkey-hide gelatin according to claim 1, characterized in that: In step 4, water is added to the pigskin in a mass ratio of 1:1, and the pH value is adjusted to 7.5 with sodium carbonate, and then enzymes are added in a mass ratio of pigskin, collagenase, and trypsin of 1:0.01:0.005, and the mixture is kept at 55°C for 3 hours and then boiled for 3 hours.

6. The method for preparing a novel donkey-hide gelatin according to claim 1, characterized in that: In step 5, the combined filtrate is added with 1-2% activated carbon by volume, stirred for 10-20 minutes, allowed to stand for 2-3 hours, filtered, and the filtrate is dried by freeze drying or spray drying to obtain the new donkey-hide gelatin.

7. Donkey-hide gelatin prepared by the preparation method according to any one of claims 1 to 6.

8. Use of the donkey-hide gelatin according to claim 7 in the preparation of anti-aging drugs.

9. Use of the donkey-hide gelatin according to claim 7 in the preparation of antioxidant drugs.

10. Use of the donkey-hide gelatin according to claim 7 in the preparation of blood pressure lowering drugs.

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