Whitening and freckle-removing essence containing ginkgo biloba extract and preparation method of whitening and freckle-removing essence

Abstract

The invention belongs to the field of essence preparation, and particularly relates to whitening and freckle-removing essence containing ginkgo biloba extract and a preparation method of the whitening and freckle-removing essence. Mild protease liquid, a ginkgo leaf extract prepared after extraction and a melanin inhibiting compound are added into an absorption-promoting transdermal complex prepared through a complexation reaction, and the obtained efficient whitening nanoparticles can efficiently whiten the skin; mussel mucoprotein and glycollic acid are combined through a non-covalent bond, the obtained freckle-removing base solution can be adhered to melanin-deposited skin and promote freckle-growing cuticle to fall off, and the freckle-removing preparation can effectively remove deposited melanin on the skin by mixing with a repairable mixture; the slow-release moisturizing solution can maintain the whitening and freckle-removing effects for a long time, the efficient whitening nanoparticles are uniformly dispersed in the mixed viscous solution, and the prepared whitening and freckle-removing secondary throwing essence containing the ginkgo biloba extract has the advantages of efficient whitening and freckle-removing, stable and lasting effect, long-term protection effect on skin, and no irritation.

Description

Technical Field

[0001] The invention belongs to the field of essence preparation, and specifically refers to a whitening and freckle-removing single-disposable essence containing ginkgo extract and a preparation method thereof. Background Art

[0002] Whitening and anti-freckle essence containing plant extracts is a skin care product that combines plant extracts with whitening ingredients. It aims to effectively improve skin pigmentation problems and brighten skin tone through the power of natural plants and scientifically formulated whitening ingredients. At the same time, it nourishes the skin and restores it to a healthy and bright state. It usually appears in the form of essence, with a light texture and easy to absorb. It can penetrate deep into the bottom layer of the skin to exert its whitening and freckle-removing effect.

[0003] Many plant extracts have strong antioxidant capacity and can effectively resist the damage of free radicals. Antioxidant ingredients can protect the skin from damage by the external environment, slow down skin aging, and maintain the youthful vitality of the skin. Essences usually contain moisturizing ingredients such as sodium hyaluronate and glycerin, which can provide sufficient moisture for the skin and keep the skin moisturized. Plant extracts can also provide long-lasting moisturizing and enhance the skin's barrier function, so that the skin can be whitened while also remaining hydrated and plump. Whitening and anti-freckle essences containing plant extracts have become an indispensable part of modern skin care due to their natural, gentle and efficient characteristics.

[0004] At present, the preparation technology of the existing whitening and freckle-removing single-use essence has the following problems: first, the whitening ingredients added to the existing whitening and freckle-removing single-use essence, such as baicalin, are easily oxidized, and the whitening and freckle-removing effect is poor, and the stability and efficacy are poor; second, the whitening and freckle-removing effect of the existing whitening and freckle-removing essence cannot be maintained for a long time after use; third, the existing whitening and freckle-removing essence is irritating to skin cells, and makes the skin sensitive and damaged after long-term use. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a whitening and freckle-removing single-use essence containing ginkgo extract and a preparation method thereof. In order to solve the problems that the existing whitening and freckle-removing single-use essence has poor whitening and freckle-removing effect, cannot maintain the effect for a long time, and damages the skin after use, the present invention uses high-efficiency whitening nanoparticles, freckle removal preparations, and sustained-release moisturizing solutions for mixing. The prepared whitening and freckle-removing single-use essence containing ginkgo extract has stable whitening and freckle-removing effect, maintains the whitening and freckle-removing effect for a long time, and is non-irritating to the skin when used for a long time.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: the present invention proposes a whitening and anti-freckle essence containing ginkgo extract, and the raw materials for preparing the whitening and anti-freckle essence containing ginkgo extract specifically include the following components in parts by weight: 22-36 parts of high-efficiency whitening nanoparticles, 35-42 parts of anti-freckle preparations, 1.8-3.2 parts of magnesium vitamin C phosphate, 1.1-1.7 parts of phenyl salicylate, and 0.8-1.2 parts of polyacrylic acid.

[0007] Preferably, the raw materials for preparing the high-efficiency whitening nanoparticles include the following components in parts by weight: 7-11 parts of ethylenediaminetetraacetic acid, 4-8 parts of calcium gluconate, 1.8-2.3 parts of mixed bacterial agent, 22-26 parts of ginkgo leaves, 2.8-4.2 parts of cyclohexenone, and 1.7-2.6 parts of undecylenoylphenylalanine.

[0008] Preferably, the raw materials for preparing the freckle removal preparation include the following components in parts by weight: 11-14 parts of mussel mucin, 1.6-3.4 parts of glycolic acid, 1-2 parts of uridine, 0.7-1.2 parts of caffeic acid phenethyl ester, 0.9-1.3 parts of hesperidin, and 0.6-0.9 parts of bitter melon polypeptide.

[0009] Preferably, the method for preparing the highly effective whitening nanoparticles comprises the following steps: S1. Put ethylenediaminetetraacetic acid, calcium gluconate and ultrapure water into a reactor with a power of 2.2-2.6 kW, react at a temperature of 50-60° C., react for 2.3-2.8 h, and react at a speed of 100-120 r / min to obtain a transdermal complex that promotes absorption; S2. Inoculate the mixed bacterial agent into a 5% sterile glucose solution, place it in a constant temperature oscillation box with a power of 1.8-2.4 kW, the constant temperature oscillation culture temperature is 28°C, the constant temperature oscillation time is 24 h, the constant temperature oscillation culture speed is 350 r / min, after the constant temperature oscillation culture, place it in an ultrasonic cell disruptor with a power of 1.2-1.5 kW, the ultrasonic disruption frequency is 20 kHz, the ultrasonic disruption temperature is 0°C, the ultrasonic disruption time is 6-8 min, and after disruption, filter it with a 0.22 mm sterile filter membrane to obtain a mild protease solution; S3, putting the ginkgo leaves into a pulverizer with a power of 1.5-1.8 kW, the pulverizing temperature is 23-28°C, the pulverizing time is 15-20 min, the pulverizing speed is 3500-4500 r / min, and after pulverizing, putting them into an ultrasonic extractor with a power of 1.3-1.6 kW with a 70% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40-45°C, the extraction time is 8-13 min, and extraction is performed to obtain a ginkgo leaf extract; S4, placing cyclohexenone, undecylenoyl phenylalanine and 50% ethanol solution in a constant temperature stirrer with a power of 1.6-1.9 kW, stirring at a constant temperature of 28-35°C, a constant temperature stirring speed of 90-110 r / min, a constant temperature stirring time of 16-28 min, and stirring at a constant temperature to obtain a melanin inhibition complex; S5, adding the mild protease solution prepared in S2 and the ginkgo leaf extract prepared in S3 into the melanin inhibition complex prepared in S4, stirring at a constant temperature of 28-35°C, a constant temperature stirring speed of 90-110 r / min, and a constant temperature stirring time of 20-26 min, stirring and mixing to obtain a highly effective whitening mixture; S6. Add the high-efficiency whitening mixture prepared by S5 to the absorption-promoting transdermal complex prepared by S1, with a stirring temperature of 23-28°C, a stirring time of 15-20 min, and a stirring speed of 300-380 r / min. After mixing, put it into a vacuum freeze-nano spray dryer with a power of 2.3-2.8 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5-3 h. Perform freeze-spray drying to obtain high-efficiency whitening nanoparticles.

[0010] Furthermore, in S1, the mass fraction of EDTA in ultrapure water is 1%.

[0011] Further, in S2, the mixed bacterial agent is composed of Heterobasidiomycetes and Aspergillus oryzae, with a weight ratio of 1.2:1. The Heterobasidiomycetes were purchased from the China General Microbiological Culture Collection Center with a preservation number of CGMCC5.1579. The Aspergillus oryzae was purchased from the China General Microbiological Culture Collection Center with a preservation number of CGMCC3.334. The solid-liquid ratio of the mixed bacterial agent to 5% sterile glucose solution is 1:100 g / mL.

[0012] Furthermore, in S3, the solid-liquid ratio of the ginkgo leaf to the 70% ethanol solution is 1:1.6 g / mL, and in S4, the mass fraction of the cyclohexenone in the 50% ethanol solution is 3-6%.

[0013] Preferably, the preparation method of the freckle removal preparation comprises the following steps: L1. Put mussel mucin, glycolic acid and ultrapure water into a stirring tank with a power of 1.5-1.8 kW, stir at a temperature of 40-50°C, for 30-40 min, at a stirring speed of 200-400 r / min, and stir to mix to obtain a freckle base solution; L2. Place uridine, caffeic acid phenethyl ester, hesperidin, bitter melon polypeptide, dimethylformamide, and ultrapure water into a stirring tank with a power of 1.5-1.8 kW, stir at a temperature of 25-35°C, stir for 15-25 min, and stir at a speed of 300-500 r / min to dissolve and obtain a restorative mixture; L3. Add the freckle removal base liquid prepared in L1 into the restorative mixture prepared in L2, stir at a temperature of 28-36°C, for 20-30 min, at a speed of 300-400 r / min, mix evenly to obtain a freckle removal preparation.

[0014] Furthermore, in L1, the mass fraction of the mussel mucin in ultrapure water is 12-18%, in L2, the mass fraction of uridine in ultrapure water is 0.8-1.4%, and the mass fraction of caffeic acid phenethyl ester in dimethylformamide is 1.1-1.3%.

[0015] The present invention also proposes a method for preparing a whitening and anti-freckle essence containing ginkgo extract, which specifically comprises the following steps: Step 1: Put magnesium vitamin C phosphate, phenyl salicylate, polyacrylic acid, and 50% ethanol solution into a magnetic stirrer with a power of 1.8-2.2 kW, stir at a temperature of 30-40°C, stir for 23-28 min, and stir at a speed of 300-500 r / min until fully dissolved to obtain a sustained-release moisturizing solution; Step 2: Add the freckle removal preparation into the sustained-release moisturizing solution prepared in step 1, stir at a temperature of 25-33°C, for 20-26 min, at a speed of 300-500 r / min, and obtain a mixed viscous solution after stirring; Step 3: Place the high-efficiency whitening nanoparticles into the mixed viscous solution prepared in step 2, use an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min. After uniform dispersion, place the mixture into a 1.4 kW ultraviolet lamp sterilizer, the ultraviolet lamp sterilization wavelength is 253.7 nm, the ultraviolet lamp sterilization temperature is 28°C, and the ultraviolet lamp sterilization time is 40 min. After sterilization by ultraviolet lamp, the mixture is packaged to obtain a whitening and anti-freckle essence containing ginkgo extract.

[0016] Preferably, in step 1, the mass fraction of the magnesium ascorbyl phosphate in the 50% ethanol solution is 3-5%.

[0017] The beneficial effects achieved by the present invention are as follows: The invention adopts ethylenediaminetetraacetic acid and calcium gluconate for mixing. Calcium gluconate can penetrate the surface layer of the skin. The ethylenediaminetetraacetic acid can form a stable complex with calcium ions and has good water solubility. The solubility in water is strong and the bioavailability is improved. The mixed bacterial agent containing island Heterobasidiomycetes and Aspergillus oryzae is subjected to constant temperature oscillation culture and ultrasonic crushing. The obtained mild protease liquid can effectively degrade the protein in the stratum corneum without causing excessive damage to other parts of the skin, reducing the thickness and roughness of the stratum corneum, allowing the effective components to penetrate deeper into the bottom layer of the skin, stimulating the metabolism of the skin, promoting the formation of new cells, and removing keratin to help improve the skin The overall texture of the skin makes it smoother, so that it can better reflect light and make the skin look brighter. The ginkgo leaf extract obtained by crushing and extracting ginkgo leaves contains quercetin, kaempferol, isorhamnetin, ginkgolide A, ginkgolide B, ginkgolide C, bilobalide, and arbutin, which can resist inflammation, antioxidant, inhibit the production of melanin, reduce the appearance of dark spots and pigmentation, interfere with melanocytes, dilute the formed melanin, inhibit allergic reactions, improve blood circulation, and promote the regeneration and repair of skin cells. Cyclohexenone and undecylenoyl phenylalanine are mixed and bound by non-covalent bonds to obtain a stable melanin inhibition complex, which can be combined with tyrosine The imidazole on the histidine residue on the tyrosinase forms a covalent bond as a melanin-stimulating hormone receptor antagonist, effectively intercepting the melanin production signal and inhibiting the activity of tyrosinase, thereby inhibiting the formation of melanin. The high-efficiency whitening mixture containing mild protease solution, ginkgo leaf extract, and melanin inhibition complex is placed in a pro-absorption transdermal complex for freeze spray drying. The obtained high-efficiency whitening nanoparticles can efficiently penetrate into the deep layer of the skin, effectively inhibit the formation and transfer of melanin, stimulate the metabolism of the skin, prevent the deposition of melanin, and effectively and persistently whiten the skin; mussel mucin contains a large amount of 3,4-dihydroxyphenylalanine, which can react with Unoxidized 3,4-dihydroxyphenylalanine generated during the formation of melanin undergoes a cross-linking reaction, thereby increasing the adhesion of the freckle base solution to the surface of melanin. The freckle base solution obtained by combining mussel mucin and glycolic acid through non-covalent bonds can adhere to the skin with melanin deposition, allowing glycolic acid to effectively penetrate into the surface of the skin and promote the shedding of the stratum corneum with spots. The restorative mixture containing uridine, caffeic acid phenethyl ester, hesperidin, and bitter melon polypeptide can repair damaged skin, promote the generation of new cells, metabolize and decompose melanin, and enhance the immunity of the barrier itself. The freckle base solution is mixed with the restorative mixture to obtain a freckle preparation that can effectively remove the melanin that has already been deposited on the skin;The phenyl salicylate solution has a strong absorption effect in the ultraviolet band, the magnesium vitamin C phosphate can reduce dopaquinone and prevent it from further converting into melanin, the flexible film formed by the polyacrylic acid solution after drying has a moisturizing effect, and the obtained sustained-release moisturizing solution can maintain the whitening and freckle removal effect for a long time, and the high-efficiency whitening nanoparticles are evenly dispersed in the mixed viscous solution. The prepared whitening and freckle removal essence containing ginkgo extract can effectively whiten and remove freckles, with stable and long-lasting effects, long-term protective effects on the skin, and no irritation. ; BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and understandable manner in conjunction with the accompanying drawings. Obviously, the drawings described below are only for the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 This is a comparison of the face of subject A before and after use for 14 days; Figure 2 This is a comparison of the face of subject B before and after use for 14 days; Figure 3 This is a result diagram of the skin brightness value described in Experimental Example 1 of the present invention; Figure 4 This is the skin melanin value result diagram described in Experimental Example 1 of the present invention; Figure 5 This is a graph showing the melanin inhibition rate results described in Experimental Example 2 of the present invention; Figure 6 This is a graph showing the tyrosinase inhibition rate results described in Experimental Example 2 of the present invention. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0021] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0022] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0023] Example 1: This example provides a whitening and anti-freckle essence containing ginkgo extract and a preparation method thereof. The whitening and anti-freckle essence containing ginkgo extract comprises the following components in parts by weight: 22 parts of high-efficiency whitening nanoparticles, 35 parts of freckle removal preparations, 1.8 parts of magnesium vitamin C phosphate, 1.1 parts of phenyl salicylate, and 0.8 parts of polyacrylic acid.

[0024] The raw materials for preparing the high-efficiency whitening nanoparticles include the following components in parts by weight: 7 parts of ethylenediaminetetraacetic acid, 4 parts of calcium gluconate, 1.8 parts of mixed bacterial agent, 22 parts of ginkgo leaves, 2.8 parts of cyclohexenone, and 1.7 parts of undecylenoylphenylalanine.

[0025] The raw materials for preparing the freckle removal preparation include the following components in parts by weight: 11 parts of mussel mucin, 1.6 parts of glycolic acid, 1 part of uridine, 0.7 parts of caffeic acid phenethyl ester, 0.9 parts of hesperidin, and 0.6 parts of bitter melon polypeptide.

[0026] The method for preparing the highly efficient whitening nanoparticles specifically comprises the following steps: S1. Put ethylenediaminetetraacetic acid, calcium gluconate and ultrapure water into a reactor with a power of 2.2 kW, react at a temperature of 50°C, a reaction time of 2.3 h, and a reaction speed of 100 r / min to obtain a transdermal complex that promotes absorption; S2. Inoculate the mixed bacterial agent into a 5% sterile glucose solution, place it in a constant temperature oscillation box with a power of 1.8 kW, the constant temperature oscillation culture temperature is 28°C, the constant temperature oscillation time is 24 h, the constant temperature oscillation culture speed is 350 r / min, after constant temperature oscillation culture, place it in an ultrasonic cell disruptor with a power of 1.2 kW, the ultrasonic disruption frequency is 20 kHz, the ultrasonic disruption temperature is 0°C, the ultrasonic disruption time is 6 min, and after disruption, filter it with a 0.22 mm sterile filter membrane to obtain a mild protease solution; S3, putting the ginkgo leaves into a pulverizer with a power of 1.5 kW, the pulverizing temperature is 23°C, the pulverizing time is 15 min, the pulverizing speed is 3500 r / min, and after pulverizing, putting them into an ultrasonic extractor with a power of 1.3 kW with a 70% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40°C, the extraction time is 8 min, and extraction is performed to obtain a ginkgo leaf extract; S4, placing cyclohexenone, undecylenoyl phenylalanine and 50% ethanol solution in a constant temperature stirrer with a power of 1.6 kW, with a constant temperature stirring temperature of 28°C, a constant temperature stirring speed of 90 r / min, a constant temperature stirring time of 16 min, and constant temperature stirring to obtain a melanin inhibition complex; S5, adding the mild protease solution prepared in S2 and the ginkgo leaf extract prepared in S3 into the melanin inhibition complex prepared in S4, stirring at a constant temperature of 28°C, a constant temperature stirring speed of 90 r / min, and a constant temperature stirring time of 20 min, stirring and mixing to obtain a highly effective whitening mixture; S6. Add the high-efficiency whitening mixture prepared by S5 to the absorption-promoting transdermal complex prepared by S1, with a stirring temperature of 23°C, a stirring time of 15 min, and a stirring speed of 300 r / min. After mixing, put it into a vacuum freeze-nano spray dryer with a power of 2.3 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5 h. Freeze-spray drying is performed to obtain high-efficiency whitening nanoparticles.

[0027] In S1, the mass fraction of EDTA in ultrapure water is 1%.

[0028] In S2, the mixed bacterial agent is composed of Heterobasidiomycetes and Aspergillus oryzae, and the weight ratio is 1.2:1. The Heterobasidiomycetes are purchased from the China General Microbiological Culture Collection Center with a preservation number of CGMCC5.1579. The Aspergillus oryzae is purchased from the China General Microbiological Culture Collection Center with a preservation number of CGMCC3.334. The solid-liquid ratio of the mixed bacterial agent to 5% sterile glucose solution is 1:100 g / mL.

[0029] In S3, the solid-liquid ratio of the ginkgo leaf to the 70% ethanol solution is 1:1.6 g / mL, and in S4, the mass fraction of the cyclohexenone in the 50% ethanol solution is 3%.

[0030] The preparation method of the freckle removal preparation specifically comprises the following steps: L1. Put mussel mucin, glycolic acid and ultrapure water into a stirring tank with a power of 1.5 kW, stir at a temperature of 40°C, stir for 30 min, and stir at a speed of 200 r / min to obtain a freckle removal base solution; L2. Place uridine, caffeic acid phenethyl ester, hesperidin, bitter melon polypeptide, dimethylformamide, and ultrapure water into a stirring tank with a power of 1.5 kW, stir at a temperature of 25°C, stir for 15 min, and stir at a speed of 300 r / min to dissolve and obtain a restorative mixture; L3. Add the freckle removal base liquid prepared in L1 into the restorative mixture prepared in L2, stir at 28°C for 20 min, stir at 300 r / min, mix evenly, and obtain the freckle removal preparation.

[0031] In L1, the mass fraction of the mussel mucin in ultrapure water was 12%, in L2, the mass fraction of uridine in ultrapure water was 0.8%, and the mass fraction of caffeic acid phenethyl ester in dimethylformamide was 1.1%.

[0032] This embodiment also provides a method for preparing a whitening and anti-freckle essence containing ginkgo extract, which specifically comprises the following steps: Step 1: Put magnesium vitamin C phosphate, phenyl salicylate, polyacrylic acid, and 50% ethanol solution into a magnetic stirrer with a power of 1.8 kW, stir at a temperature of 30°C, stir for 23 min, and stir at a speed of 300 r / min until fully dissolved to obtain a sustained-release moisturizing solution; Step 2: put the freckle removal preparation into the sustained-release moisturizing solution prepared in step 1, stirring at a temperature of 25°C, a stirring time of 20 min, a stirring speed of 300 r / min, and obtain a mixed viscous solution after stirring; Step 3: Place the high-efficiency whitening nanoparticles into the mixed viscous solution prepared in step 2, use an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min. After uniform dispersion, place the mixture into a 1.4 kW ultraviolet lamp sterilizer, the ultraviolet lamp sterilization wavelength is 253.7 nm, the ultraviolet lamp sterilization temperature is 28°C, and the ultraviolet lamp sterilization time is 40 min. After sterilization by ultraviolet lamp, the mixture is packaged to obtain a whitening and anti-freckle essence containing ginkgo extract.

[0033] In step 1, the mass fraction of the magnesium ascorbyl phosphate in the 50% ethanol solution is 3%.

[0034] Example 2: This example provides a whitening and anti-freckle essence containing ginkgo extract and a preparation method thereof. The whitening and anti-freckle essence containing ginkgo extract comprises the following components in parts by weight: 32 parts of high-efficiency whitening nanoparticles, 38 parts of freckle removal preparations, 2.6 parts of magnesium vitamin C phosphate, 1.3 parts of phenyl salicylate, and 1.1 parts of polyacrylic acid.

[0035] The raw materials for preparing the high-efficiency whitening nanoparticles include the following components in parts by weight: 9 parts of ethylenediaminetetraacetic acid, 6 parts of calcium gluconate, 2.2 parts of mixed bacterial agent, 23 parts of ginkgo leaves, 3.6 parts of cyclohexenone, and 2.3 parts of undecylenoylphenylalanine.

[0036] The raw materials for preparing the freckle removal preparation include the following components in parts by weight: 12 parts of mussel mucin, 2.8 parts of glycolic acid, 1.5 parts of uridine, 1.1 parts of caffeic acid phenethyl ester, 1.2 parts of hesperidin, and 0.7 parts of bitter melon polypeptide.

[0037] The method for preparing the highly efficient whitening nanoparticles specifically comprises the following steps: S1. Put ethylenediaminetetraacetic acid, calcium gluconate and ultrapure water into a reactor with a power of 2.4 kW, react at a temperature of 55°C, a reaction time of 2.6 h, and a reaction speed of 110 r / min to obtain a transdermal complex that promotes absorption; S2. The mixed bacterial agent was inoculated into a 5% sterile glucose solution, and placed in a constant temperature oscillation box with a power of 2.2 kW. The constant temperature oscillation culture temperature was 28°C, the constant temperature oscillation time was 24 h, and the constant temperature oscillation culture speed was 350 r / min. After the constant temperature oscillation culture, it was placed in an ultrasonic cell disruptor with a power of 1.3 kW, the ultrasonic disruption frequency was 20 kHz, the ultrasonic disruption temperature was 0°C, and the ultrasonic disruption time was 7 min. After disruption, it was filtered using a 0.22 mm sterile filter membrane to obtain a mild protease solution; S3, putting the ginkgo leaves into a pulverizer with a power of 1.6 kW, the pulverizing temperature is 26°C, the pulverizing time is 17 min, the pulverizing speed is 4000 r / min, and after pulverizing, putting them into an ultrasonic extractor with a power of 1.4 kW with a 70% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 43°C, the extraction time is 11 min, and extraction is performed to obtain a ginkgo leaf extract; S4, placing cyclohexenone, undecylenoyl phenylalanine and 50% ethanol solution in a constant temperature stirrer with a power of 1.7 kW, with a constant temperature stirring temperature of 32°C, a constant temperature stirring speed of 100 r / min, a constant temperature stirring time of 22 min, and constant temperature stirring to obtain a melanin inhibition complex; S5, adding the mild protease solution prepared in S2 and the ginkgo leaf extract prepared in S3 into the melanin inhibition complex prepared in S4, stirring at a constant temperature of 33°C, a constant temperature stirring speed of 100 r / min, and a constant temperature stirring time of 23 min, stirring and mixing to obtain a highly effective whitening mixture; S6. Add the high-efficiency whitening mixture prepared by S5 to the absorption-promoting transdermal complex prepared by S1, with a stirring temperature of 26°C, a stirring time of 18 min, and a stirring speed of 360 r / min. After mixing, put it into a vacuum freeze-nano spray dryer with a power of 2.6 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.8 h. Freeze-spray drying is performed to obtain high-efficiency whitening nanoparticles.

[0038] In S1, the mass fraction of EDTA in ultrapure water is 1%.

[0039] In S2, the mixed bacterial agent is composed of Heterobasidiomycetes and Aspergillus oryzae, and the weight ratio is 1.2:1. The Heterobasidiomycetes are purchased from the China General Microbiological Culture Collection Center with a preservation number of CGMCC5.1579. The Aspergillus oryzae is purchased from the China General Microbiological Culture Collection Center with a preservation number of CGMCC3.334. The solid-liquid ratio of the mixed bacterial agent to 5% sterile glucose solution is 1:100 g / mL.

[0040] In S3, the solid-liquid ratio of the ginkgo leaf to the 70% ethanol solution is 1:1.6 g / mL, and in S4, the mass fraction of the cyclohexenone in the 50% ethanol solution is 4%.

[0041] The preparation method of the freckle removal preparation specifically comprises the following steps: L1. Put mussel mucin, glycolic acid and ultrapure water into a stirring tank with a power of 1.6 kW, stir at a temperature of 45°C, stir for 35 min, and stir at a speed of 300 r / min to obtain a freckle removal base solution; L2. Put uridine, caffeic acid phenethyl ester, hesperidin, bitter melon polypeptide, dimethylformamide and ultrapure water into a stirring tank with a power of 1.6 kW, stir at a temperature of 30°C, stir for 20 min, and stir at a speed of 400 r / min, and stir to dissolve to obtain a restorative mixture; L3. Add the freckle removal base liquid prepared in L1 into the restorative mixture prepared in L2, stir at 32°C for 25 min, stir at 300 r / min, mix evenly, and obtain the freckle removal preparation.

[0042] In L1, the mass fraction of the mussel mucin in ultrapure water is 15%, in L2, the mass fraction of uridine in ultrapure water is 1.2%, and the mass fraction of caffeic acid phenethyl ester in dimethylformamide is 1.2%.

[0043] This embodiment also provides a method for preparing a whitening and anti-freckle essence containing ginkgo extract, which specifically comprises the following steps: Step 1: Put magnesium vitamin C phosphate, phenyl salicylate, polyacrylic acid, and 50% ethanol solution into a magnetic stirrer with a power of 1.9 kW, stir at a temperature of 34°C, stir for 26 min, and stir at a speed of 400 r / min until fully dissolved to obtain a sustained-release moisturizing solution; Step 2: Add the freckle removal preparation into the sustained-release moisturizing solution prepared in step 1, stir at a temperature of 28°C, for 22 min, at a stirring speed of 400 r / min, and obtain a mixed viscous solution after stirring; Step 3: Place the high-efficiency whitening nanoparticles into the mixed viscous solution prepared in step 2, use an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min. After uniform dispersion, place the mixture into a 1.4 kW ultraviolet lamp sterilizer, the ultraviolet lamp sterilization wavelength is 253.7 nm, the ultraviolet lamp sterilization temperature is 28°C, and the ultraviolet lamp sterilization time is 40 min. After sterilization by ultraviolet lamp, the mixture is packaged to obtain a whitening and anti-freckle essence containing ginkgo extract.

[0044] In step 1, the mass fraction of the magnesium ascorbyl phosphate in the 50% ethanol solution is 4%.

[0045] Example 3: This example provides a whitening and anti-freckle essence containing ginkgo extract and a preparation method thereof. The whitening and anti-freckle essence containing ginkgo extract comprises the following components in parts by weight: 36 parts of high-efficiency whitening nanoparticles, 42 parts of freckle removal preparations, 3.2 parts of magnesium vitamin C phosphate, 1.7 parts of phenyl salicylate, and 1.2 parts of polyacrylic acid.

[0046] The raw materials for preparing the high-efficiency whitening nanoparticles include the following components in parts by weight: 11 parts of ethylenediaminetetraacetic acid, 8 parts of calcium gluconate, 2.3 parts of mixed bacterial agent, 26 parts of ginkgo leaves, 4.2 parts of cyclohexenone, and 2.6 parts of undecylenoylphenylalanine.

[0047] The raw materials for preparing the freckle removal preparation include the following components in parts by weight: 14 parts of mussel mucin, 3.4 parts of glycolic acid, 2 parts of uridine, 1.2 parts of caffeic acid phenethyl ester, 1.3 parts of hesperidin, and 0.9 parts of bitter melon polypeptide.

[0048] The method for preparing the highly efficient whitening nanoparticles specifically comprises the following steps: S1. Put ethylenediaminetetraacetic acid, calcium gluconate and ultrapure water into a reactor with a power of 2.6 kW, react at a temperature of 60°C, a reaction time of 2.8 h, and a reaction speed of 120 r / min to obtain a transdermal complex that promotes absorption; S2. The mixed bacterial agent was inoculated into a 5% sterile glucose solution, and placed in a constant temperature oscillation box with a power of 2.4 kW. The constant temperature oscillation culture temperature was 28°C, the constant temperature oscillation time was 24 h, and the constant temperature oscillation culture speed was 350 r / min. After the constant temperature oscillation culture, it was placed in an ultrasonic cell disruptor with a power of 1.5 kW, the ultrasonic disruption frequency was 20 kHz, the ultrasonic disruption temperature was 0°C, and the ultrasonic disruption time was 8 min. After disruption, it was filtered using a 0.22 mm sterile filter membrane to obtain a mild protease solution; S3, putting the ginkgo leaves into a pulverizer with a power of 1.8 kW, the pulverizing temperature is 28°C, the pulverizing time is 20 min, the pulverizing speed is 4500 r / min, and after pulverizing, putting them into an ultrasonic extractor with a power of 1.6 kW with a 70% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 45°C, the extraction time is 13 min, and extraction is performed to obtain a ginkgo leaf extract; S4, placing cyclohexenone, undecylenoyl phenylalanine and 50% ethanol solution in a constant temperature stirrer with a power of 1.9 kW, with a constant temperature stirring temperature of 35°C, a constant temperature stirring speed of 110 r / min, a constant temperature stirring time of 28 min, and constant temperature stirring to obtain a melanin inhibition complex; S5, adding the mild protease solution prepared in S2 and the ginkgo leaf extract prepared in S3 into the melanin inhibition complex prepared in S4, stirring at a constant temperature of 35°C, a constant temperature stirring speed of 110 r / min, and a constant temperature stirring time of 26 min, stirring and mixing to obtain a highly effective whitening mixture; S6. Add the high-efficiency whitening mixture prepared by S5 to the absorption-promoting transdermal complex prepared by S1, with the stirring temperature at 28°C, the stirring time at 20 min, and the stirring speed at 380 r / min. After mixing, put it into a vacuum freeze-nano spray dryer with a power of 2.8 kW, the vacuum degree at -0.05 MPa, the freeze-nano spray drying temperature at -40°C, the freeze-nano spray drying particle size at 100 nm, and the freeze-nano spray drying time at 3 h. Freeze-spray drying is performed to obtain high-efficiency whitening nanoparticles.

[0049] In S1, the mass fraction of EDTA in ultrapure water is 1%.

[0050] In S2, the mixed bacterial agent is composed of Heterobasidiomycetes and Aspergillus oryzae, and the weight ratio is 1.2:1. The Heterobasidiomycetes are purchased from the China General Microbiological Culture Collection Center with a preservation number of CGMCC5.1579. The Aspergillus oryzae is purchased from the China General Microbiological Culture Collection Center with a preservation number of CGMCC3.334. The solid-liquid ratio of the mixed bacterial agent to 5% sterile glucose solution is 1:100 g / mL.

[0051] In S3, the solid-liquid ratio of the ginkgo leaf to the 70% ethanol solution is 1:1.6 g / mL, and in S4, the mass fraction of the cyclohexenone in the 50% ethanol solution is 6%.

[0052] The preparation method of the freckle removal preparation specifically comprises the following steps: L1. Put mussel mucin, glycolic acid and ultrapure water into a stirring tank with a power of 1.8 kW, stir at a temperature of 50°C, stir for 40 min, and stir at a speed of 400 r / min to obtain a freckle removal base solution; L2. Place uridine, caffeic acid phenethyl ester, hesperidin, bitter melon polypeptide, dimethylformamide, and ultrapure water into a stirring tank with a power of 1.8 kW, stir at a temperature of 35°C, stir for 25 min, and stir at a speed of 500 r / min to dissolve and obtain a restorative mixture; L3. Add the freckle removal base liquid prepared in L1 into the restorative mixture prepared in L2, stir at 36°C for 30 min, stir at 400 r / min, mix evenly, and obtain the freckle removal preparation.

[0053] In L1, the mass fraction of the mussel mucin in ultrapure water was 18%, in L2, the mass fraction of uridine in ultrapure water was 1.4%, and the mass fraction of caffeic acid phenethyl ester in dimethylformamide was 1.3%.

[0054] This embodiment also provides a method for preparing a whitening and anti-freckle essence containing ginkgo extract, which specifically comprises the following steps: Step 1: Put magnesium vitamin C phosphate, phenyl salicylate, polyacrylic acid, and 50% ethanol solution into a magnetic stirrer with a power of 2.2 kW, stir at a temperature of 40°C, stir for 28 min, and stir at a speed of 500 r / min until fully dissolved to obtain a sustained-release moisturizing solution; Step 2: put the freckle removal preparation into the sustained-release moisturizing solution prepared in step 1, stirring at a temperature of 33°C, a stirring time of 26 min, and a stirring speed of 500 r / min to obtain a mixed viscous solution; Step 3: Place the high-efficiency whitening nanoparticles into the mixed viscous solution prepared in step 2, use an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min. After uniform dispersion, place the mixture into a 1.4 kW ultraviolet lamp sterilizer, the ultraviolet lamp sterilization wavelength is 253.7 nm, the ultraviolet lamp sterilization temperature is 28°C, and the ultraviolet lamp sterilization time is 40 min. After sterilization by ultraviolet lamp, the mixture is packaged to obtain a whitening and anti-freckle essence containing ginkgo extract.

[0055] In step 1, the mass fraction of the magnesium ascorbyl phosphate in the 50% ethanol solution is 5%.

[0056] Comparative Example 1: This comparative example provides a whitening and freckle-removing essence and a preparation method thereof, which is different from Example 1 only in that the added high-efficiency whitening nanoparticles do not contain melanin-inhibiting complexes, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0057] Comparative Example 2: This comparative example provides a whitening and freckle-removing essence and a preparation method thereof, which is different from Example 1 only in that the added freckle-removing preparation does not contain a freckle-removing base liquid, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0058] Experimental Example 1: Whitening effect measurement test.

[0059] The whitening effect test steps of the whitening and anti-freckle essence containing ginkgo extract prepared in Examples 1-3 of the present invention are as follows: (1) 60 subjects aged 38-48 years old were randomly selected and divided into 6 groups, with 10 subjects in each group. The subjects' back skin was irradiated with a solar simulator once a day for 4 consecutive days. After 4 days of blackening, the initial skin brightness value and initial skin melanin value of the subjects' back skin were measured using a SkinGlossMeter (skin brightness measuring instrument) and a laser skin detector, respectively. After recording, the whitening and freckle-removing essence containing ginkgo extract prepared in Example 1-3 was applied to the back skin of the subjects in Groups 1-3 of Example 1-3, the whitening and freckle-removing essence prepared in Comparative Example 1-2 was applied to the back skin of the subjects in Group 1-2 of Comparative Example 1-2, and the commercially available whitening and freckle-removing essence (purchased from Guangzhou Yunmei Cosmetics Co., Ltd.) in the control group was applied to the back skin of the subjects in the control group. Each person applied 2 mL each time on an area of ​​6 cm 2 The skin area was applied once a day. After 4 weeks of use, the skin brightness value and skin melanin value of the back of the subjects in the Example 1-3 group, the Comparative Example 1-2 group, and the control group were measured again using the SkinGlossMeter (skin brightness measuring instrument) and the laser skin detector, and recorded; (2) After 4 weeks of use, stop using the whitening and anti-freckle essence containing ginkgo extract prepared in Example 1-3, the whitening and anti-freckle essence prepared in Comparative Example 1-2, and the commercially available whitening and anti-freckle essence in the control group (purchased from Guangzhou Yunmei Cosmetics Co., Ltd.) for 7 days, and then measure the skin brightness value and skin melanin value of the back of the subjects in Groups 1-3, Comparative Example 1-2, and the control group again after stopping use, and record them.

[0060] Result analysis: Figure 1 This is a comparison of the face of subject A before and after using it for 14 days. Figure 2 This is a comparison of the face of subject B before and after use for 14 days. Figure 3 This is a result graph of the skin brightness value described in Experimental Example 1 of the present invention. As shown in the figure, the initial values ​​of the back skin brightness of the subjects in Example 1-3 groups were 25.87, 25.80, and 25.89, respectively. After applying the whitening and freckle-removing essence containing ginkgo extract prepared in Example 1-3 for 4 weeks, the values ​​of the back skin brightness of the subjects in Example 1-3 groups after testing were 33.69, 34.59, and 35.72, respectively. After stopping the application for 7 days, the values ​​of the back skin brightness of the subjects in Example 1-3 groups after stopping use were 33.52, 34.34, and 35.52, respectively. The initial values ​​of the back skin brightness of the subjects in Comparative Example 1-2 groups were 25.81 and 25.89, respectively. After applying the whitening and freckle-removing essence prepared in Comparative Example 1-2 for 4 weeks, the values ​​of the back skin brightness of the subjects in Comparative Example 1-2 groups after testing were 30.30 and 31.27, respectively. After stopping the application for 7 days, the back skin brightness of the subjects in Comparative Example 1-2 groups after testing were 31. After 4 days, the values ​​of the back skin brightness of the subjects in the control group 1-2 were 29.11 and 30.60 respectively, and the initial value of the back skin brightness of the subjects in the control group was 25.71. After 4 weeks of application of the commercially available whitening and anti-freckle essence in the control group, the value of the back skin brightness of the subjects in the control group was 28.21 after the test. After 7 days of stopping the application, the value of the back skin brightness of the subjects in the control group was 26.00. Figure 4This is the result graph of the skin melanin value described in Experimental Example 1 of the present invention. The initial values ​​of melanin on the back of the subjects in Groups 1-3 of Examples are 171.14, 170.62, and 169.77, respectively. After applying the whitening and anti-freckle essence containing ginkgo extract prepared in Examples 1-3 for 4 weeks, the values ​​of melanin on the back of the subjects in Groups 1-3 of Examples are 110.42, 102.77, and 97.70, respectively. After stopping the application for 7 days, the values ​​of melanin on the back of the subjects in Groups 1-3 of Examples are 110.14, 102.46, and 97.43, respectively. The initial values ​​of melanin on the back of the subjects in Groups 1-2 of Comparative Examples are 170.48 and 170.90, respectively. After applying the whitening and anti-freckle essence prepared in Comparative Examples 1-2 for 4 weeks, the values ​​of melanin on the back of the subjects in Groups 1-2 of Comparative Examples are 125.34 and 121.53, respectively. After stopping the application for 7 days, the values ​​of melanin on the back of the subjects in Groups 1-2 of Comparative Examples are 130. d later, the values ​​of melanin on the back skin of the subjects in the comparison groups 1-2 after stopping use were 125.97 and 121.56 respectively, the initial value of melanin on the back skin of the subjects in the control group was 170.27, and after smearing the commercially available whitening and freckle-removing essence in the control group for 4 weeks, the value of melanin on the back skin of the subjects in the control group after testing was 145.13, and 7 d after stopping application, the value of melanin on the back skin of the subjects in the control group after stopping use was 161.07, indicating that the whitening and freckle-removing essence containing ginkgo extract prepared by the present invention can whiten efficiently, has a stable and lasting effect, and has a significant skin lightening effect.

[0061] Experimental Example 2: Test on the inhibitory effect on melanin.

[0062] The test steps for determining the inhibitory effect of the whitening and anti-freckle essence containing ginkgo extract prepared in Examples 1-3 of the present invention on melanin are as follows: (1) B16-F10 cells were cultured at 2×10 5 The cells were inoculated into a 6-well plate at a density of 10 cells / well. After 48 hours, the well plate was taken out, the culture medium in the well was discarded, and 2 mL of the whitening and anti-freckle essence containing ginkgo extract prepared in Example 1-3, the whitening and anti-freckle essence prepared in Comparative Example 1-2, and the commercially available whitening and anti-freckle essence in the control group (purchased from Guangzhou Yunmei Cosmetics Co., Ltd.) were added to the corresponding wells. Three parallels were set for each group, and a blank control was set at the same time. Then, the incubation continued for 48 hours. After the culture was completed, the cells were lysed, the intracellular melanin was extracted by centrifugation, 200 mL of 1 mol / L sodium hydroxide solution containing 10% DMSO was added, and then placed in an 80°C water bath and heated for 3 hours. After the melanin was completely dissolved, 180 mL was drawn into a 96-well plate, and the absorbance value at 492 nm was detected. The melanin inhibition rate was calculated according to the formula: melanin inhibition rate = [(OD 空白组 -OD 实验组 ) / OD 空白组]×100%, record; (2) B16-F10 cells were seeded into 96-well plates at a density of 8,000 cells / well and then placed at 37°C with 5% CO. 2 , incubated in a saturated humidity incubator for 48 h. After 48 h, the 96-well plate was taken out, the culture medium in the wells was discarded, and 2 mL of the whitening and anti-freckle essence containing ginkgo extract prepared in Example 1-3, the whitening and anti-freckle essence prepared in Comparative Example 1-2, and the commercially available whitening and anti-freckle essence in the control group (purchased from Guangzhou Yunmei Cosmetics Co., Ltd.) were added to the corresponding wells, 3 parallels were set for each group, and a blank control was set at the same time, and the incubation continued for 24 h. After 24 h, the plate was taken out and the old culture medium was discarded. Each well was washed with 100 mL PBS. Then, 40 mL of cell lysis solution containing 1 mM PMSF was added to each well. The plate was placed at 4°C for lysis for 30 min. After the lysis, the plate was placed in a 37°C incubator for 5 min. Then, 100 mL of 1 mg / mL L-DOPA was added to each well and the cells were incubated in a 37°C incubator for 2 h. After the incubation, the absorbance at 492 nm was measured using an ELISA reader. The tyrosinase inhibition rate was calculated according to the following formula: tyrosinase inhibition rate = [(OD 空白组 -OD 实验组 ) / OD 空白组 ]×100%.

[0063] Result analysis: Figure 5 This is a result graph of melanin inhibition rate described in Experimental Example 2 of the present invention. As shown in the figure, the whitening and freckle-removing essence containing ginkgo extract prepared in Examples 1-3 were incubated with B16-F10 cells for 48 hours, respectively, and the inhibition rates of melanin reached 82.43%, 83.59%, and 84.32%, respectively. The whitening and freckle-removing essence prepared in Comparative Examples 1-2 were incubated with B16-F10 cells for 48 hours, respectively, and the inhibition rates of melanin reached 55.79% and 80.04%, respectively. The commercially available whitening and freckle-removing essence in the control group was incubated with B16-F10 cells for 48 hours, and the inhibition rate of melanin was 29.54%. Figure 6This is a graph showing the tyrosinase inhibition rate results of Experimental Example 2 of the present invention. As shown in the figure, the whitening and freckle-removing essence containing ginkgo extract prepared in Examples 1-3 were incubated with B16-F10 cells for 24 h, respectively, and the inhibition rates of tyrosinase reached 73.95%, 75.10%, and 76.07%, respectively. The whitening and freckle-removing essence prepared in Comparative Examples 1-2 were incubated with B16-F10 cells for 24 h, respectively, and the inhibition rates of tyrosinase reached 58.82% and 71.75%, respectively. The commercially available whitening and freckle-removing essence in the control group was incubated with B16-F10 cells for 24 h, and the inhibition rate of tyrosinase was 31.98%, indicating that the whitening and freckle-removing essence containing ginkgo extract prepared in the present invention can effectively remove freckles and has a significant effect of inhibiting melanin production.

[0064] Experimental Example 3: Irritation to the skin.

[0065] The steps of the test for the irritation test of the whitening and anti-freckle essence containing ginkgo extract prepared in Examples 1-3 of the present invention on white guinea pig skin are as follows: (1) Sixty healthy white male guinea pigs weighing 700 g were randomly divided into 6 groups of 10; (2) Conventional hair removal was performed on both sides of the back of white guinea pigs, with a hair removal area of ​​3 cm × 3 cm. After 24 hours of hair removal, the local skin was observed to be intact and without damage. The experiment used the left and right sides of the same guinea pig for self-comparison. The left side of the back of the guinea pigs in the Example 1-3 group was smeared with the whitening and freckle-removing essence containing ginkgo extract prepared in Example 1-3, the left side of the back of the guinea pigs in the comparative example 1-2 group was smeared with the whitening and freckle-removing essence prepared in comparative example 1-2, and the left side of the back of the guinea pigs in the control group was smeared with the control group commercial whitening and freckle-removing essence (purchased from Guangzhou Yunmei Cosmetics Co., Ltd.), applied twice a day, 2 mL each time, and after continuous use for 12 days, the treated area was cleaned with warm water and observed; (3) Observe with the naked eye 24, 48, and 72 hours after cleaning with warm water to see if there is any erythema or edema on the treated area on the left side of the guinea pig's back. Calculate the average score of each group of guinea pigs at each observation time point according to Table 1, and evaluate the intensity of stimulation according to Table 2.

[0066] Table 1. Skin irritation response scoring criteria

[0067] Table 2. Skin irritation intensity evaluation criteria

[0068] Table 3. Skin irritation reaction results

[0069] Result analysis: As shown in Table 3, the physiological structure of guinea pig skin is similar to that of human skin. No erythema and edema appeared on the backs of the white guinea pigs in Examples 1-3 and Comparative Examples 1-2, while mild irritation appeared on the backs of the white guinea pigs in the control group. This indicates that the whitening and anti-freckle essence containing ginkgo extract prepared by the present invention has excellent biocompatibility, is non-toxic and non-irritating to the skin, and has a long-term protective effect on the skin.

[0070] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0071] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A whitening and anti-freckle essence containing ginkgo extract, wherein the raw materials for preparing the whitening and anti-freckle essence containing ginkgo extract specifically include the following components in parts by weight: 22-36 parts of high-efficiency whitening nanoparticles, 35-42 parts of anti-freckle preparations, 1.8-3.2 parts of magnesium vitamin C phosphate, 1.1-1.7 parts of phenyl salicylate, and 0.8-1.2 parts of polyacrylic acid; The raw materials for preparing the high-efficiency whitening nanoparticles include the following components in parts by weight: 7-11 parts of ethylenediaminetetraacetic acid, 4-8 parts of calcium gluconate, 1.8-2.3 parts of mixed bacterial agent, 22-26 parts of ginkgo biloba leaves, 2.8-4.2 parts of cyclohexenone, and 1.7-2.6 parts of undecylenoylphenylalanine; The raw materials for preparing the freckle removal preparation include the following components in parts by weight: 11-14 parts of mussel mucin, 1.6-3.4 parts of glycolic acid, 1-2 parts of uridine, 0.7-1.2 parts of caffeic acid phenethyl ester, 0.9-1.3 parts of hesperidin, and 0.6-0.9 parts of bitter melon polypeptide.

2. The whitening and anti-freckle essence containing ginkgo extract according to claim 1, characterized in that: The method for preparing the highly efficient whitening nanoparticles specifically comprises the following steps: S1, mixing ethylenediaminetetraacetic acid, calcium gluconate and ultrapure water to obtain a transdermal complex that promotes absorption; S2, inoculating the mixed bacterial agent into a 5% sterile glucose solution, culturing with shaking, and then ultrasonically disrupting and filtering to obtain a mild protease solution; S3, crushing the ginkgo leaves and extracting them with 70% ethanol solution to obtain a ginkgo leaf extract; S4, stirring and mixing cyclohexenone, undecylenoyl phenylalanine and 50% ethanol solution to obtain a melanin inhibition complex; S5, adding the mild protease solution prepared in S2 and the ginkgo leaf extract prepared in S3 into the melanin inhibition complex prepared in S4, stirring and mixing to obtain a highly effective whitening mixture; S6. Add the high-efficiency whitening mixture prepared in S5 to the absorption-promoting transdermal complex prepared in S1, mix, and then perform freeze spray drying to obtain high-efficiency whitening nanoparticles.

3. The whitening and anti-freckle essence containing ginkgo extract according to claim 2, characterized in that: In S1, the mass fraction of EDTA in ultrapure water is 1%.

4. The whitening and anti-freckle essence containing ginkgo extract according to claim 3, characterized in that: In S2, the mixed bacterial agent is composed of Heterobasidiomycetes and Aspergillus oryzae, and the mixing ratio by weight is 1.2:

1. The solid-liquid ratio of the mixed bacterial agent to 5% sterile glucose solution is 1:100 g / mL.

5. The whitening and anti-freckle essence containing ginkgo extract according to claim 4, characterized in that: In S3, the solid-liquid ratio of the ginkgo leaf to the 70% ethanol solution is 1:1.6 g / mL, and in S4, the mass fraction of the cyclohexenone in the 50% ethanol solution is 3-6%.

6. The whitening and anti-freckle essence containing ginkgo extract according to claim 5, characterized in that: The preparation method of the freckle removal preparation specifically comprises the following steps: L1. Stir and mix mussel mucin, glycolic acid and ultrapure water to obtain a freckle removal base solution; L2, stirring uridine, caffeic acid phenethyl ester, hesperidin, bitter melon polypeptide, dimethylformamide, and ultrapure water until dissolved to obtain a restorative mixture; L3. Add the freckle removal base liquid prepared in L1 into the restorative mixture prepared in L2, mix well, and obtain the freckle removal preparation.

7. The whitening and anti-freckle essence containing ginkgo extract according to claim 6, characterized in that: In L1, the mass fraction of the mussel mucin in ultrapure water is 12-18%, in L2, the mass fraction of uridine in ultrapure water is 0.8-1.4%, and the mass fraction of caffeic acid phenethyl ester in dimethylformamide is 1.1-1.3%.

8. A method for preparing the whitening and anti-freckle serum containing ginkgo extract according to any one of claims 1 to 7, characterized in that: The specific steps include: Step 1: fully dissolve magnesium vitamin C phosphate, phenyl salicylate, polyacrylic acid, and 50% ethanol solution to obtain a sustained-release moisturizing solution; Step 2, putting the freckle removal preparation into the sustained-release moisturizing solution prepared in step 1, and stirring to obtain a mixed viscous solution; Step 3: Evenly disperse the highly effective whitening nanoparticles in the mixed viscous solution prepared in step 2, sterilize with ultraviolet light, and then package to obtain a single-use essence containing ginkgo extract for whitening and removing spots.

9. The method for preparing the whitening and anti-freckle essence containing ginkgo extract according to claim 8, characterized in that: In step 1, the mass fraction of the magnesium ascorbyl phosphate in the 50% ethanol solution is 3-5%.

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