Sorting device for cellular immunomagnetic beads

Abstract

The invention provides a cell immunomagnetic bead sorting device, and belongs to the technical field of magnetic bead sorting, the cell immunomagnetic bead sorting device comprises a U-shaped containing frame, one end of a telescopic piece is connected with a moving plate, one end of the moving plate is rotatably connected with the U-shaped containing frame, one end of the U-shaped containing frame is rotatably provided with a rotating rod, and the rotating rod is rotatably connected with a support. A first fixing frame is slidably installed in the U-shaped containing frame, a containing plate is fixedly installed on the first fixing frame, a magnet is slidably installed on one side of the U-shaped containing frame, a connecting rod is fixedly installed at one end of the first fixing frame, and a trapezoidal block is slidably installed on the connecting rod. And a wedge-shaped block is slidably installed on the inner side wall of the U-shaped containing frame, a first spring is installed between the wedge-shaped block and the inner side wall of the U-shaped containing frame, through back-and-forth movement of the telescopic part, mixed liquid in the test tubes on the containing plate can be fully and evenly mixed, and the situation that due to the fact that the mixed liquid is left on test tube covers, sorting is not thorough can be avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of magnetic bead sorting, in particular to a sorting device for cell immune magnetic beads. Background Art

[0002] The immunomagnetic bead method for separating cells is based on the ability of cell surface antigens to bind to specific monoclonal antibodies connected to magnetic beads. In an external magnetic field, cells connected to magnetic beads through antibodies are adsorbed and retained in the magnetic field. Cells without such surface antigens cannot bind to specific monoclonal antibodies connected to magnetic beads and therefore have no magnetism and do not stay in the magnetic field, which is the supernatant, thereby allowing the cells to be separated.

[0003] When sorting a small number of separation samples, manual operation is still the main method. However, when mixing, the samples are generally picked up manually and shaken up and down vigorously. However, this mixing method will cause a variety of cells to remain on the inner wall and cover of the test tube. When magnetic bead sorting is performed on this basis, the sorting will be incomplete. Summary of the invention

[0004] In view of the above-mentioned deficiencies in the prior art, the technical problem to be solved by the embodiments of the present invention is to provide a cell immune magnetic bead sorting device.

[0005] To solve the above technical problems, the present invention provides the following technical solutions: A sorting device for cell immune magnetic beads, comprising a placement plate, a magnet, and a U-shaped accommodating frame, a movable plate, a telescopic member, and a bottom plate, wherein one end of the telescopic member is connected to the movable plate, and the other end of the telescopic member is mounted on the bottom plate, a bracket is fixedly mounted on the bottom plate, and the movable plate is slidably connected to the bottom plate, and one end of the movable plate away from the telescopic member is rotatably connected to the U-shaped accommodating frame, and a rotating rod is rotatably mounted on one end of the U-shaped accommodating frame away from the movable plate, and an end of the rotating rod away from the U-shaped accommodating frame is rotatably connected to the bracket, a first fixed frame is slidably mounted in the U-shaped accommodating frame, a placement plate is fixedly mounted on the first fixed frame, and a plurality of first test tube slots are provided on the placement plate, a magnet is slidably mounted on one side of the U-shaped accommodating frame, a connecting rod is fixedly mounted on one end of the first fixed frame, a trapezoidal block is slidably mounted on the connecting rod, a wedge block is slidably mounted on the inner side wall of the U-shaped accommodating frame, and a first spring is mounted between the wedge block and the inner side wall of the U-shaped accommodating frame.

[0006] As a further improvement: a second spring is installed between the trapezoidal block and the first fixing frame.

[0007] As a further improvement scheme: a support plate is fixedly mounted on the trapezoidal block.

[0008] As a further improvement scheme: a plurality of second test tube slots corresponding to the first test tube slots are formed on the support plate, and the inner diameter of the slot openings of the second test tube slots is smaller than the inner diameter of the slot openings of the first test tube slots.

[0009] As a further improvement scheme: a second fixing frame is fixedly mounted on one end of the connecting rod away from the first fixing frame, and one end of the second fixing frame is in an arc shape.

[0010] As a further improvement scheme: a fixing rod is fixedly installed on the second fixing frame, a hollow tube is installed on the bottom plate, a first piston plate is slidably installed in the hollow tube, a conical block is installed on the first piston plate, a connecting tube is fixedly installed on the U-shaped containing frame, a second piston plate is slidably installed in the connecting tube, the second piston plate is fixedly connected to the magnet, the connecting tube is connected to the hollow tube through a hose, and the connecting tube, the hollow tube and the hose are filled with liquid medium.

[0011] As a further improvement scheme: the contact surface between the fixing rod and the conical block is a curved surface.

[0012] As a further improvement, two U-shaped accommodating frames are provided.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows: by providing a telescopic part, the reciprocating movement of the telescopic part can drive the rotating rod to rotate, and finally the U-shaped containing frame presents a large-angle swing in two directions, so that the mixed liquid in the test tube on the placement plate can be fully mixed, and manual shaking up and down can be avoided, and the residual mixed liquid on the test tube cover can be avoided, resulting in incomplete sorting. By providing a wedge block and a slidably installed trapezoidal block, the first fixed frame together with the connecting rod can be inserted into the U-shaped containing frame, and the wedge block is inserted between the connecting rod and the trapezoidal block, so as to fix the position of the first fixed frame together with the connecting rod. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 A schematic diagram of the overall structure of a cell immune magnetic bead sorting device Figure 1 ;

[0015] Figure 2 A schematic diagram of the overall structure of a cell immune magnetic bead sorting device Figure 2 ;

[0016] Figure 3 It is a partial structural schematic diagram of a cell immune magnetic bead sorting device;

[0017] Figure 4 It is a schematic diagram of the cross-sectional structure of a wedge block of a cell immune magnetic bead sorting device;

[0018] Figure 5 It is a schematic diagram of the cross-sectional structure of a hollow tube and a connecting tube of a cell immune magnetic bead sorting device;

[0019] In the figure: 1, bottom plate; 2, telescopic member; 21, force plate; 22, moving plate; 23, mounting plate; 3, hollow tube; 31, conical block; 32, first connecting column; 33, first piston plate; 4, connecting pipe; 41, guide plate; 42, magnet; 43, sliding block; 44, second connecting column; 45, second piston plate; 5, U-shaped accommodating frame; 51, supporting frame; 52, wedge block; 53, sliding groove; 54, first spring; 6, placing plate; 61, first test tube groove; 7, supporting plate; 71, second test tube groove; 8, fixing rod; 9, first fixing frame; 91, trapezoidal block; 92, second spring; 93, second fixing frame; 94, connecting rod; 10, test tube; 101, flange; 102, test tube cover; 11, bracket; 12, rotating rod. DETAILED DESCRIPTION

[0020] The technical solution of the present application is further described in detail below in conjunction with specific implementation methods.

[0021] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be understood as limiting the present application.

[0022] See also Figures 1 to 5 In one embodiment, a cell immune magnetic bead sorting device includes a placement plate 6 and a magnet 42, characterized in that it also includes a U-shaped accommodating frame 5, a movable plate 22, a telescopic member 2, and a bottom plate 1, wherein one end of the telescopic member 2 is connected to the movable plate 22, and the other end of the telescopic member 2 is installed on the bottom plate 1, and a bracket 11 is fixedly installed on the bottom plate 1, and the movable plate 22 is slidably connected to the bottom plate 1, and the end of the movable plate 22 away from the telescopic member 2 is rotatably connected to the U-shaped accommodating frame 5, and the end of the U-shaped accommodating frame 5 away from the movable plate 22 is rotatably installed with a rotating rod 12, and the rotating rod 12 is rotatably connected to the bracket 11 at one end away from the U-shaped accommodating frame 5, a first fixing frame 9 is slidably installed in the U-shaped accommodating frame 5, a placing plate 6 is fixedly installed on the first fixing frame 9, a plurality of first test tube grooves 61 are provided on the placing plate 6, a magnet 42 is slidably installed on one side of the U-shaped accommodating frame 5, a connecting rod 94 is fixedly installed at one end of the first fixing frame 9, a trapezoidal block 91 is slidably installed on the connecting rod 94, a wedge block 52 is slidably installed on the inner side wall of the U-shaped accommodating frame 5, and a first spring 54 is installed between the wedge block 52 and the inner side wall of the U-shaped accommodating frame 5.

[0023] In this embodiment, mounting plates 23 are fixedly installed on both sides of one end of the movable plate 22 away from the telescopic member 2, and fixed shafts (not shown in the figure) are fixedly installed on the two mounting plates 23, and the fixed shafts pass through the U-shaped accommodating frame 5 and are rotatably connected to the U-shaped accommodating frame 5. One end of the rotating rod 12 is rotatably connected to the bracket 11, and the other end of the rotating rod 12 is rotatably connected to one side of the middle section of the U-shaped accommodating frame 5. A sliding groove 53 is provided on the U-shaped accommodating frame 5, and a guide plate 41 is fixedly installed on one side of the magnet 42. The guide plate 4 A sliding block 43 is fixedly installed at one end, the sliding block 43 is located in the sliding groove 53, and the sliding block 43 is slidably connected to the sliding groove 53. The cross section of the trapezoidal block 91 is an octagon. The connecting rod 94 passes through the side of the trapezoidal block 91, and the connecting rod 94 is slidably connected to the trapezoidal block 91. The cross section of the wedge block 52 is a right triangle, and the inclined surface of the wedge block 52 matches the hypotenuse of the trapezoidal block 91. A cover body is hingedly installed on the placement plate 6, and a distance is left between the cover body and the test tube cover 102.

[0024] When performing immunomagnetic bead sorting, a test tube 10 made of plastic is generally used for experimental extraction. The test tube 10 is generally cylindrical at one end and conical at the other end, wherein the upper end of the cylinder is rotatably connected to a test tube cover 102, and the end of the cylinder is also provided with a flange 101 for being placed on a placement plate 6. During the sorting process, it is necessary to mix the cell mixture and wash the magnetic beads. By providing a telescopic part 2, the reciprocating movement of the telescopic part 2 can drive the rotating rod 12 to rotate, and finally the U-shaped accommodating frame 5 presents a large-angle swing in two directions, so that the placement plate can be adjusted. 6, the mixed liquid in the test tube 10 is fully mixed, and it is possible to avoid manual shaking up and down to avoid residual mixed liquid on the test tube cover 102, which leads to incomplete sorting. First, the test tube 10 is placed on the first placement slot, and then the first fixing frame 9 is inserted into the U-shaped accommodating frame 5. In addition, by providing a wedge block 52 and a slidably mounted trapezoidal block 91, the first fixing frame 9 together with the connecting rod 94 can be inserted into the U-shaped accommodating frame 5, and the wedge block 52 is inserted between the connecting rod 94 and the trapezoidal block 91, so as to fix the position of the first fixing frame 9 together with the connecting rod 94.

[0025] See also Figures 1 to 4 In one embodiment, two U-shaped receiving frames 5 are provided.

[0026] In this embodiment, a movable plate 22 is installed on each of the two U-shaped accommodating frames 5, and a force-bearing plate 21 is fixedly installed on each of the two movable plates 22. The force-bearing plate 21 is connected to the telescopic member 2, and the two movable plates 22 are connected through the force-bearing plate 21, so as to realize the driving of the two U-shaped accommodating frames 5. Two first fixing frames 9 and a connecting rod 94 are slidably installed on each of the two U-shaped accommodating frames 5, and the placement plate 6 and the support plate 7 are installed between the two first fixing frames 9. By providing two U-shaped accommodating frames 5, the placement plate 6 can be connected through the two first fixing frames 9, and a plurality of test tubes 10 can be mixed at the same time, and the stability of the placement plate 6 can also be increased.

[0027] See also Figures 1 to 4 In one embodiment, a second spring 92 is installed between the trapezoidal block 91 and the first fixing frame 9 .

[0028] In this embodiment, by providing a second spring 92, when the first fixing frame 9 is not inserted into the U-shaped accommodating frame 5, the trapezoidal block 91 is close to the end of the connecting rod 94 away from the first fixing frame 9. When inserting, the wedge block 52 is inserted between the first fixing frame 9 and the connecting rod 94. At this time, the trapezoidal block 91 moves upward under the action of the wedge block 52. When it is necessary to separate the first fixing frame 9 from the U-shaped accommodating frame 5, the U-shaped accommodating frame 5 is manually pressed. At this time, the spring is squeezed, and the wedge block 52 moves and retracts along the inclined surface of the trapezoidal block 91, and then is located between the upper end of the trapezoidal block 91 and the first fixing frame 9, driving the first fixing frame 9 to move upward. During the upward movement, the trapezoidal block 91 is pushed to fit with the other end of the connecting rod 94. The wedge block 52 can now retract into the U-shaped accommodating frame 5, thereby completing the removal of the first fixing frame 9.

[0029] See also Figures 1 to 5 In one embodiment, a support plate 7 is fixedly mounted on the trapezoidal block 91 .

[0030] In this embodiment, by providing a support plate 7, the other end of the test tube 10 can be supported. While the U-shaped containing frame 5 swings in one direction, a spring is provided to allow the trapezoidal block 91 to rock on the connecting rod 94, so that the rocking can be transmitted to the inside of the test tube 10 through the support plate 7, thereby achieving a better mixing effect.

[0031] See also Figure 2 , Figure 5 In one embodiment, the support plate 7 is provided with a plurality of second test tube slots 71 corresponding to the first test tube slots 61 , and the inner diameter of the slot of the second test tube slots 71 is smaller than the inner diameter of the slot of the first test tube slots 61 .

[0032] In this embodiment, by providing a second test tube slot 71, the position of the other end of the test tube 10 can be fixed, and by setting the inner diameter of the slot of the second test tube slot 71 to be smaller than the inner diameter of the slot of the first test tube slot 61, the inner diameter of the second test tube slot 71 can be matched with the other end of the test tube 10.

[0033] See also Figures 1 to 5 In one embodiment, a second fixing frame 93 is fixedly mounted on one end of the connecting rod 94 away from the first fixing frame 9, and one end of the second fixing frame 93 is in an arc shape.

[0034] In this embodiment, by providing a second arc-shaped fixing frame 93, the wedge block 52 can be pushed to retract into the U-shaped accommodating frame 5 when the first fixing frame 9 moves downward. Secondly, an inclined surface matching the trapezoidal block 91 is provided on the other end of the second fixing frame 93, so that when the first fixing frame 9 needs to be pulled out, the trapezoidal block 91 can be completely attached to the second fixing frame 93, thereby facilitating the retraction of the wedge block 52.

[0035] See also Figures 1 to 5 In one embodiment, a fixing rod 8 is fixedly mounted on the second fixing frame 93, a hollow tube 3 is mounted on the bottom plate 1, a first piston plate 33 is slidably mounted in the hollow tube 3, a conical block 31 is mounted on the first piston plate 33, a connecting tube 4 is fixedly mounted on the U-shaped accommodating frame 5, a second piston plate 45 is slidably mounted in the connecting tube 4, the second piston plate 45 is fixedly connected to the magnet 42, the connecting tube 4 is connected to the hollow tube 3 through a hose, and the connecting tube 4, the hollow tube 3 and the hose are filled with liquid medium.

[0036] In this embodiment, a second connecting column 44 is installed between the first piston plate 33 and the conical block 31, a first connecting column 32 is fixedly installed between the second piston plate 45 and the guide plate 41, the connecting pipe 4, the hollow tube 3 and the hose are filled with hydraulic oil, the vertical section of the conical block 31 is trapezoidal, a support frame 51 is fixedly installed on the U-shaped containing frame 5, and a connecting pipe 4 is installed on the support frame 51.

[0037] When it is necessary to mix the mixed liquid, the first fixing frame 9 is inserted into the U-shaped containing frame 5. After the insertion, the wedge block 52 reaches between the second fixing frame 93 and the trapezoidal block 91, and at this time the trapezoidal block 91 has a small displacement. At this time, the first fixing frame 9, the connecting rod 94, and the second fixing frame 93 are all fixed in position, and then the telescopic member 2 is driven to drive the U-shaped containing frame 5. When magnetic bead sorting is required after the mixing is completed, the first fixing frame 9 is manually pressed, the spring is squeezed, and the wedge block 52 moves and retracts along the inclined surface of the trapezoidal block 91. Then the wedge block 52 is located between the upper end of the trapezoidal block 91 and the first fixing frame 9. During the pressing process, the trapezoidal block 91 first moves upward relative to the placement plate 6, and then moves downward. At this time, when pressing When the conical block 31 is moved, the fixing rod 8 squeezes the conical block 31 and drives the magnet 42 to approach the test tube 10 through the action of the hydraulic oil. At this time, the telescopic part 2 can be closed for static sorting. After the sorting is completed, the test tube cover 102 is opened to remove the supernatant, and the reagent is continuously injected to clean the magnetic beads. At this time, the telescopic part 2 is continued to be driven to drive the test tube 10 to swing, mix and clean. During the swinging process, when the connecting rod 94 is separated from the conical block 31, the magnet 42 is away from the test tube 10. At this time, the conical block 31 returns to its original position. After the mixing is completed, the U-shaped containing frame 5 is moved to a vertical position. At this time, the connecting rod 94 contacts the conical block 31 until the conical block 31 is pushed again to move until the magnet 42 is close to the test tube 10, thereby performing the magnetic bead sorting operation.

[0038] When the first fixing frame 9 is taken out, the first fixing frame 9 is driven to move upward. During the upward movement, the trapezoidal block 91 is pushed to fit with the other end of the second fixing frame 93. The wedge block 52 can be retracted into the U-shaped accommodating frame 5 at this time, thereby completing the removal of the first fixing frame 9.

[0039] See also Figure 5 In one embodiment, the contact surface between the fixing rod 8 and the conical block is an arc surface.

[0040] In this embodiment, the arc surface is provided to facilitate the contact between the conical block 31 and the fixing rod 8 and drive the conical block 31 to move.

[0041] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-limiting from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention, and any reference numerals in the claims should not be regarded as limiting the claims involved.

[0042] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A cell immune magnetic bead sorting device, comprising a placement plate and a magnet, characterized in that: The cam is provided with a plurality of support members, each of which has a plurality of support members, each of which has a plurality of support members, each of which has a plurality of support members, each of which has a plurality of support members.

2. A cell immune magnetic bead sorting device according to claim 1, characterized in that: A second spring is installed between the trapezoidal block and the first fixing frame.

3. A cell immune magnetic bead sorting device according to claim 2, characterized in that: A support plate is fixedly mounted on the trapezoidal block.

4. A cell immune magnetic bead sorting device according to claim 3, characterized in that: The support plate is provided with a plurality of second test tube slots corresponding to the first test tube slots, and the inner diameter of the slot openings of the second test tube slots is smaller than the inner diameter of the slot openings of the first test tube slots.

5. A cell immune magnetic bead sorting device according to any one of claims 1 to 4, characterized in that: A second fixing frame is fixedly mounted on one end of the connecting rod away from the first fixing frame, and one end of the second fixing frame is in an arc shape.

6. A cell immune magnetic bead sorting device according to claim 5, characterized in that: A fixing rod is fixedly installed on the second fixing frame, a hollow tube is installed on the bottom plate, a first piston plate is slidably installed in the hollow tube, a conical block is installed on the first piston plate, a connecting tube is fixedly installed on the U-shaped containing frame, a second piston plate is slidably installed in the connecting tube, the second piston plate is fixedly connected to the magnet, the connecting tube is connected to the hollow tube through a hose, and the connecting tube, the hollow tube and the hose are filled with liquid medium.

7. A cell immune magnetic bead sorting device according to claim 6, characterized in that: The contact surface between the fixing rod and the conical block is an arc surface.

8. The cell immune magnetic bead sorting device according to claim 1, characterized in that: Two U-shaped accommodating frames are provided.

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