A sterilization filtration device and method for preparing a sterile culture medium

Abstract

The present invention relates to the technical field of culture medium preparation, and particularly relates to a sterilization and filtration device and method for preparing a sterile culture medium. It includes: a housing, the housing is fixedly connected with an input pipe, the input pipe is fixedly connected and communicated with a first control valve, the first control valve is fixedly connected and communicated with two upper connecting pipes, the upper connecting pipes are fixedly connected and communicated with a filter cylinder, the filter cylinder is fixedly connected and communicated with a lower connecting pipe, the two lower connecting pipes are jointly fixedly connected and communicated with a second control valve, and the second control valve is fixedly connected and communicated with an output pipe; a filter screen, there are two, respectively installed on adjacent upper connecting pipes, and a flow-pushing member is rotatably connected to the upper connecting pipe; a driving impeller, rotatably connected to the input pipe. By driving the surrounding solution to rotate slowly through the flow-pushing member, the bacteria accumulated in the filter screen are moved to positions not in contact with the filter screen, thereby reducing the situation of filter screen blockage caused by bacteria and prolonging the single use time of the filter screen.

Description

Technical Field

[0001] The present invention relates to the technical field of culture medium preparation, and particularly relates to a sterilization filtration device and method for preparing a sterile culture medium. Background Art

[0002] After the existing culture medium solution is prepared, it is necessary to use a composite sterilization filter element of 0.45μm + 0.22μm for sterilization filtration in order to screen out bacteria in the culture medium and produce a sterile culture medium solution. The method of sterilization through a composite sterilization filter element can ensure that the culture medium is free from bacterial contamination while minimizing the nutritional loss of the culture medium (for example, some culture medium solutions contain vitamin components, and the vitamin components will decompose at high temperatures, so this part of the culture medium solution is not suitable for methods such as high-temperature disinfection, and this part of the culture medium solution usually uses a composite sterilization filter element for sterilization filtration). However, due to the overly fine filter diameter of the composite sterilization filter element used for sterilization filtration, when the solution passes through the composite sterilization filter element, a large amount of bacteria removed accumulate in the composite sterilization filter element, resulting in problems such as a gradual decrease in the filtration speed of the solution and easy blockage of the composite sterilization filter element, resulting in low filtration efficiency. Summary of the Invention

[0003] In order to overcome the disadvantages that the existing composite sterilization filter element usually has a gradually decreasing filtration efficiency and is prone to blockage when sterilizing and filtering a culture medium solution, the present invention provides a sterilization filtration device and method for preparing a sterile culture medium.

[0004] The technical solution of the present invention is as follows: A sterilization filtration device for preparing a sterile culture medium, comprising: a housing, the housing is fixedly connected with an input pipe, the input pipe is fixedly connected and communicated with a first control valve, the first control valve is fixedly connected and communicated with two upper connecting pipes, the upper connecting pipes are fixedly connected and communicated with a filter cylinder, the filter cylinder is fixedly connected and communicated with a lower connecting pipe, the two lower connecting pipes are jointly fixedly connected and communicated with a second control valve, and the second control valve is fixedly connected and communicated with an output pipe; a filter screen, there are two, and they correspond to the two upper connecting pipes one by one, are respectively installed on the adjacent upper connecting pipes and are located inside the adjacent filter cylinders, the upper connecting pipe is rotatably connected with a flow-pushing member, and the flow-pushing member is used to push the liquid and bacteria at the inner wall of the filter screen to rotate slowly; a driving fan, rotatably connected to the input pipe, and a speed measurement module for detecting its own rotation speed is installed thereon, and a belt pulley and belt drive are provided between the two flow-pushing members and the driving fan; a collection mechanism, arranged inside the housing, for collecting bacteria in the filter screen.

[0005] Further, the flow-pushing member is composed of a cylinder and vertically arranged plates distributed circumferentially.

[0006] Further, the collection mechanism includes: collection shells, there are two of them, corresponding to the two filter cartridges one by one, respectively installed on the adjacent filter cartridges, the collection shells are fixedly connected to the filter net, and the collection shells are fixedly connected with collection filter nets; backwashing components, there are two of them, corresponding to the two filter cartridges one by one, both are arranged in the outer shell, and are used to backwash the filter net when the adjacent filter nets are blocked.

[0007] Further, the backwashing component includes: a liquid storage barrel, the liquid storage barrel is fixedly connected in the outer shell; a first piston rod, slidably connected to the adjacent liquid storage barrel, the first piston rod divides the upper cavity and the lower cavity in the liquid storage barrel, the upper cavity of the liquid storage barrel is communicated with the adjacent collection shell, and the lower cavity of the liquid storage barrel is communicated with the adjacent lower connecting pipe; an interception component, arranged on the adjacent liquid storage barrel, and is used to intercept and collect bacteria entering the adjacent liquid storage barrel.

[0008] Further, the interception component includes: a filter shell, rotatably connected in the adjacent liquid storage barrel; a moving part, slidably connected to the adjacent first piston rod, and the moving part is used to drive the adjacent filter shell to rotate; a first hydraulic telescopic rod, fixedly connected in the adjacent liquid storage barrel, and the telescopic end of the first hydraulic telescopic rod is fixedly connected to the adjacent moving part.

[0009] Further, it also includes: a driving component, arranged in the outer shell, and is used to drive the two first hydraulic telescopic rods to work in sequence. The driving component includes: a driving cylinder, fixedly connected in the outer shell; a driving part, slidably connected in the outer shell, the driving part divides the driving cylinder into a first cavity and a second cavity, the first cavity in the driving cylinder is communicated with one of the upper connecting pipes, and the second cavity in the driving cylinder is communicated with the other upper connecting pipe; a fixing frame, fixedly connected to the two liquid storage barrels, a transmission gear is installed on the fixing frame, and both of the first piston rods are meshed with the transmission gear on the fixing frame through racks, and the driving part is fixedly connected to one of the first piston rods.

[0010] Further, it also includes: a pressure regulating component, arranged on the fixing frame, and is used to reduce the pressure in the upper connecting pipe that needs to be backwashed. The pressure regulating component includes: negative pressure cylinders, there are two of them, both are fixedly connected to the fixing frame, a second piston rod is slidably connected in the negative pressure cylinder, the negative pressure cylinder is communicated with the adjacent upper connecting pipe, the valve rod of the first control valve is fixedly connected with two fixing parts, and the fixing parts are slidably connected to the adjacent second piston rod.

[0011] Further, the volume of the negative pressure cylinder is larger than the volume of the driving cylinder.

[0012] Further, it further includes: two second hydraulic telescopic rods, which are respectively fixedly connected to adjacent negative pressure cylinders. A spring is installed between the telescopic end of the second hydraulic telescopic rod and its fixed part. The second piston rod is used to squeeze the telescopic end of the adjacent second hydraulic telescopic rod. The first hydraulic telescopic rod communicates with the adjacent second hydraulic telescopic rod.

[0013] A sterilization filtration method for preparing a sterile culture medium, which is applied to the sterilization filtration device for preparing a sterile culture medium as described above. The specific method is as follows:

[0014] S1: Control the first control valve and the second control valve to switch their working states so that they communicate with the same filter cartridge.

[0015] S2: Introduce a solution into the input pipe. After the solution passes through the filter screen in the filter cartridge in use, it is discharged outward. The solution simultaneously fills the adjacent liquid storage barrel and the driving barrel. The flow pusher drives the surrounding solution and bacteria to rotate, and the collection filter screen collects the bacteria in the adjacent filter screen.

[0016] S3: The driving member moves under the pressure difference between the two filter cartridges on both sides, so that the first piston rod in the working state moves to the upper side, and the other first piston rod moves to the lower side. At the same time, the valve rod drives the two fixing members to rotate, so that the second piston rod in the working state moves to the lower side, and the other second piston rod moves to the upper side.

[0017] S4: When the filter screen in the working state becomes blocked, control the first control valve and the second control valve to switch their working states so that the solution enters the other filter cartridge for filtration.

[0018] S5: After switching the state of the first control valve, the two second piston rods move towards each other. The two filter housings rotate respectively driven by the adjacent second piston rods. The two first piston rods move towards each other to backwash the blocked filter screen. The filter housing on the backwashing side intercepts the bacteria falling off the blocked filter screen.

[0019] S6: After the solution filtration is completed, clean the filter cartridge, the liquid storage barrel and the filter housing.

[0020] The beneficial effects of the present invention compared with the prior art are: the present invention drives the surrounding solution to rotate slowly through the flow pusher, so that the bacteria accumulated in the filter screen move to a position not in contact with the filter screen, thereby reducing the situation of filter screen blockage caused by bacteria and prolonging the single use time of the filter screen.

[0021] The present invention stores a part of the solution in the liquid storage barrel in advance. When the filter screen is severely blocked, the flow path of the solution is switched. On the premise of not affecting the filtration of the solution, the solution in the liquid storage barrel is used to backwash the filter screen, thereby cleaning the filter screen and restoring the use function of the filter screen.

[0022] The present invention creates negative pressure through a negative pressure cylinder. Meanwhile, by utilizing the pressure difference within two filter cylinders, it automatically drives a solution to clean a clogged filter net and collects the bacteria cleaned from the filter net, thereby increasing the automation level of the device, saving the energy and time spent on manual cleaning, and expanding the scope of application of the device. Description of the Drawings

[0023] Figure 1 is a three-dimensional structural schematic diagram of the present invention;

[0024] Figure 2 is a three-dimensional structural schematic diagram of the first control valve, upper connection pipe, and filter cylinder of the present invention;

[0025] Figure 3 is a cross-sectional view of the filter cylinder and liquid storage barrel of the present invention;

[0026] Figure 4 is a three-dimensional structural schematic diagram of the filter net, bottom plate, and flow pusher of the present invention;

[0027] Figure 5 is a cross-sectional view of the input pipe, upper connection pipe, and filter cylinder of the present invention;

[0028] Figure 6 is a cross-sectional view of the filter cylinder, collection shell, and liquid storage barrel of the present invention;

[0029] Figure 7 is a three-dimensional structural schematic diagram of the liquid storage barrel, first piston rod, and filter shell of the present invention;

[0030] Figure 8 is a three-dimensional structural schematic diagram of the first hydraulic telescopic rod and the second hydraulic telescopic rod of the present invention;

[0031] Figure 9 is a three-dimensional structural schematic diagram of the filter shell, drive cylinder, and drive member of the present invention;

[0032] Figure 10 is a three-dimensional structural schematic diagram of the first control valve, upper connection pipe, and negative pressure cylinder of the present invention.

[0033] Names and serial numbers of components in the figure: 1 - housing, 101 - input pipe, 102 - first control valve, 1021 - valve stem, 103 - upper connection pipe, 104 - filter cylinder, 105 - lower connection pipe, 106 - second control valve, 107 - output pipe, 2 - filter net, 201 - bottom plate, 202 - flow pusher, 203 - drive fan, 3 - collection shell, 301 - collection filter net, 302 - liquid storage barrel, 303 - first piston rod, 304 - filter shell, 305 - moving member, 306 - first hydraulic telescopic rod, 4 - drive cylinder, 401 - drive member, 5 - fixing frame, 501 - negative pressure cylinder, 502 - second piston rod, 503 - fixing member, 504 - second hydraulic telescopic rod. Detailed implementation mode

[0034] In the description of the present invention, it should also be noted that, unless otherwise clearly specified and defined, the terms "installation" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or a connection assisted by components. The term "communication" should be understood in a broad sense. For example, it can be a direct communication or a communication through a hose or a connecting pipe. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0035] Embodiment 1: A sterilization and filtration device for preparing a sterile culture medium, referring to Figures 1-5 , including: a housing 1, the housing 1 is fixedly connected with an input pipe 101, the input pipe 101 is fixedly connected and communicated with a first control valve 102, the first control valve 102 is fixedly connected and communicated with two upper connecting pipes 103, the upper connecting pipes 103 are fixedly connected and communicated with a filter cylinder 104, the filter cylinder 104 is fixedly connected and communicated with a lower connecting pipe 105, the two lower connecting pipes 105 are jointly fixedly connected and communicated with a second control valve 106, and the second control valve 106 is fixedly connected and communicated with an output pipe 107; two filter meshes 2, corresponding to the two upper connecting pipes 103 one by one, are respectively installed on the adjacent upper connecting pipes 103 and are located inside the adjacent filter cylinders 104. A flow-pushing member 202 is rotatably connected to the upper connecting pipe 103, and the flow-pushing member 202 is used to push the liquid and bacteria at the inner wall of the filter mesh 2 to rotate slowly; a driving impeller 203 is rotatably connected to the input pipe 101, and a speed measurement module for detecting its own rotation speed is installed thereon. A belt drive is provided between the two flow-pushing members 202 and the driving impeller 203; a collection mechanism is arranged inside the housing 1 for collecting bacteria in the filter mesh 2.

[0036] Further, referring to Figure 5 , the flow-pushing member 202 is composed of a cylinder and vertically arranged plates distributed circumferentially.

[0037] In this embodiment, aiming at the problems that the existing composite sterilization filter element usually has a gradually decreasing filtration efficiency and is prone to blockage when sterilizing and filtering the culture medium solution, the first control valve 102 and the second control valve 106 can be electrically controlled valves or hydraulic control valves. In this embodiment, the first control valve 102 and the second control valve 106 are electrically controlled valves. The first control valve 102 and the second control valve 106 are electrically connected to the control terminal. The first control valve 102 and the second control valve 106 are only connected to the same filter cartridge 104 at the same time. In this embodiment, in the initial state, both the first control valve 102 and the second control valve 106 are connected to the filter cartridge 104 on the left; the cylinder body of the filter cartridge 104 is set as an easily disassembled structure, which is convenient for the staff to disassemble the filter cartridge 104 and replace the internal filter screen 2 when needed; the filter screen 2 is jointly composed of a composite filter screen in a cylindrical structure and a bottom plate 201 at the bottom of the filter screen; the speed measurement module for detecting its own rotation speed on the driving fan 203 is an existing electrically controlled module, and this speed measurement module is electrically connected to the control terminal. The control terminal judges the flow rate of the solution in the input pipe 101 by detecting the rotation speed of the driving fan 203, and then judges whether the filter screen 2 in the current use state is severely blocked. The flow pushing member 202 is composed of a cylinder and vertically distributed vertical plates. When the flow pushing member 202 rotates, it only drives the surrounding solution to rotate circumferentially and will not guide the surrounding solution in the vertical direction, thereby promoting the solution to uniformly pass through the filter screen 2 for filtration. The flow pushing member 202 drives the bacteria to slowly flow circumferentially by pushing the surrounding solution, reducing the probability of bacteria accumulating on the inner side of the filter screen 2.

[0038] The working process is as follows: When the staff sterilizes and filters the solution, the solution is introduced into the input pipe 101. Because both the first control valve 102 and the second control valve 106 are connected to the filter cartridge 104 on the left in the initial state, the solution flows along the input pipe 101, the first control valve 102 and the upper connecting pipe 103 on the left into the filter cartridge 104 on the left. After the solution enters the filter cartridge 104 on the left, it passes through the filter screen 2 on the left and flows into the lower connecting pipe 105 on the left. The solution flows into the existing sterile collection bucket through the path of the lower connecting pipe 105 on the left, the second control valve 106 to the output pipe 107.

[0039] When the solution flows through the input pipe 101, the solution flows and drives the driving fan 203 to rotate. The control terminal monitors the rotation speed of the driving fan 203 through the speed measurement module on the driving fan 203. During the rotation of the driving fan 203, two flow-pushing members 202 are driven to rotate together through a pulley belt. The right flow-pushing member 202 rotates idly, and when the left flow-pushing member 202 rotates, it drives the surrounding solution to rotate slowly. When the solution around the left flow-pushing member 202 rotates, it continuously flushes the adjacent filter net 2 along a spiral path, so that the bacteria intercepted on part of the filter net 2 move along the inner surface of the filter net 2. The collection mechanism takes the opportunity to collect the bacteria moving along the inner surface of the left filter net 2, reducing the content of bacteria in the left filter net 2 and the probability of bacteria getting stuck in the mesh holes of the filter net 2, thus facilitating the solution to pass through the left filter net 2 and drain outwards.

[0040] As the usage time of the left filter net 2 gradually increases, the bacteria stuck in the mesh holes of the left filter net 2 gradually increase, and the left filter net 2 becomes severely blocked. At this time, the speed of the solution passing through the left filter net 2 slows down, the flow rate of the solution at the input pipe 101 slows down, and the rotation speed of the driving fan 203 slows down synchronously. Because the speed measurement module on the driving fan 203 feeds back the rotation speed of the driving fan 203 to the control terminal in real time, when the rotation speed of the driving fan 203 slows down to a certain value (the value of this rotation speed can be adjusted by the staff according to different working conditions and requirements), the control terminal controls the first control valve 102 and the second control valve 106 to work simultaneously. The first control valve 102 and the second control valve 106 are simultaneously disconnected from the left filter cylinder 104 and connected to the right filter cylinder 104. At this time, the right filter net 2 filters the solution through the same principle as above, while the collection mechanism on the left cleans and collects the bacteria intercepted in the left filter net 2, so that when the right filter net 2 becomes severely blocked, the first control valve 102 and the second control valve 106 can be switched back to the initial state again to continuously carry out sterilization filtration on the solution.

[0041] When the staff has filtered all the solutions, the staff cleans the device and completely clears out the bacteria collected by the collection mechanism.

[0042] Further, referring to Figures 3-6 , the collection mechanism includes: two collection shells 3, which correspond to the two filter cylinders 104 one by one and are respectively installed on the adjacent filter cylinders 104. The collection shell 3 is fixedly connected to the filter net 2, and the collection shell 3 is fixedly connected with a collection filter net 301; two backwashing components, which correspond to the two filter cylinders 104 one by one and are both arranged in the outer shell 1, and are used for backwashing the filter net 2 when the adjacent filter net 2 is blocked.

[0043] Further, referring to Figure 2 、 Figure 3 and Figures 5-7, the recoil component includes: a liquid storage barrel 302, which is fixedly connected to the housing 1; a first piston rod 303, which is slidably connected to the adjacent liquid storage barrel 302. The first piston rod 303 divides the interior of the liquid storage barrel 302 into an upper cavity and a lower cavity. The upper cavity of the liquid storage barrel 302 is communicated with the adjacent collection shell 3, and the lower cavity of the liquid storage barrel 302 is communicated with the adjacent lower connecting pipe 105; an interception assembly, which is arranged on the adjacent liquid storage barrel 302 and is used to intercept and collect bacteria entering the adjacent liquid storage barrel 302.

[0044] In this embodiment, the surface of the collection shell 3 close to the axis side of the adjacent filter screen 2 is connected to the inner surface of the adjacent filter screen 2, which is convenient for bacteria to flow along the inner surface of the filter screen 2 into the adjacent collection shell 3. The collection filter screen 301 is inclined (the inclination direction is as Figure 5 shown). The flow pusher 202 rotates clockwise (from top to bottom) driven by the driving fan 203. The flow pusher 202 and the collection filter screen 301 cooperate to jointly prevent bacteria in the collection shell 3 from flowing to the side away from the collection shell 3 during the process of following the solution flow; the volume of the liquid storage barrel 302 is larger than the volume of the adjacent filter cylinder 104; because the upper cavity of the liquid storage barrel 302 is communicated with the adjacent collection shell 3 and the lower cavity of the liquid storage barrel 302 is communicated with the adjacent lower connecting pipe 105, when the staff injects the solution into the adjacent filter cylinder 104, both cavities in the liquid storage barrel 302 store the solution. The barrel body of the liquid storage barrel 302 is set as an easily disassembled structure, which is convenient for the staff to take out the bacteria filtered and collected in the liquid storage barrel 302. And an exhaust valve and a drain valve are arranged in the lower cavity of the liquid storage barrel 302 (the exhaust valve and the drain valve are existing devices not shown in the figure). The exhaust valve on the liquid storage barrel 302 is used to smoothly discharge the air in the liquid storage barrel 302 when the solution enters the liquid storage barrel 302 during the initial process of replenishing the solution into the adjacent filter cylinder 104. The drain valve on the liquid storage barrel 302 is used to manually discharge the filtered solution stored in the liquid storage barrel 302 when the staff cleans the liquid storage barrel 302. The staff manually discharges the filtered solution into the sterile collection barrel.

[0045] Further, referring to Figures 6-9 , the interception assembly includes: a filter shell 304, which is rotatably connected to the adjacent liquid storage barrel 302; a moving part 305, which is slidably connected to the adjacent first piston rod 303, and the moving part 305 is used to drive the adjacent filter shell 304 to rotate; a first hydraulic telescopic rod 306, which is fixedly connected to the adjacent liquid storage barrel 302, and the telescopic end of the first hydraulic telescopic rod 306 is fixedly connected to the adjacent moving part 305.

[0046] Further, referring to Figure 7 and Figure 9, further comprising: a driving component, disposed within the housing 1, for sequentially driving the two first hydraulic telescopic rods 306 to operate. The driving component includes: a driving cylinder 4, fixedly connected within the housing 1; a driving member 401, slidably connected within the housing 1. The driving member 401 divides the interior of the driving cylinder 4 into a first cavity and a second cavity. The first cavity within the driving cylinder 4 communicates with one of the upper connecting pipes 103, and the second cavity within the driving cylinder 4 communicates with the other upper connecting pipe 103; a fixing frame 5, fixedly connected to the two liquid storage barrels 302. A transmission gear is installed on the fixing frame 5. Both first piston rods 303 are engaged with the transmission gear on the fixing frame 5 through racks. The driving member 401 is fixedly connected to one of the first piston rods 303.

[0047] In this embodiment, the specific shape of the filter housing 304 is not limited. The filter housing 304 can be circular or square. Its bottom and side walls can be flexible or a rigid filter mesh structure. The filter housing 304 is used to filter the solution entering the liquid storage barrel 302 from the collection housing 3, thereby further collecting the bacteria collected within the collection housing 3. An arc-shaped groove is provided on the outer side of the moving member 305. When the moving member 305 moves up and down, adjacent filter housings 304 rotate along the arc-shaped groove on the moving member 305. And in this embodiment, the first hydraulic telescopic rod 306 is communicated with an external hydraulic system for controlling the telescopic end of the first hydraulic telescopic rod 306 to extend downward or retract; the volume of the driving cylinder 4 is much smaller than the volume of the filter cylinder 104. The driving cylinder 4 is only used to detect the pressure difference between the two filter cylinders 104 and will not significantly affect the pressure difference between the two filter cylinders 104. The first cavity of the driving cylinder 4 is located on the upper side of the driving cylinder 4, and the second cavity of the driving cylinder 4 is located on the lower side of the driving cylinder 4. The first cavity of the driving cylinder 4 communicates with the left upper connecting pipe 103, and the second cavity of the driving cylinder 4 communicates with the right upper connecting pipe 103. And an exhaust valve and a drain valve are also provided within the driving cylinder 4 (the exhaust valve and the drain valve are existing devices not shown in the figure); the transmission gear on the fixing frame 5 is located between the two first piston rods 303. The racks on the two first piston rods 303 are respectively located on the left and right sides of the transmission gear on the fixing frame 5. The transmission gear on the fixing frame 5 is used to drive the other first piston rod to move in the opposite direction when one first piston rod moves up and down. In this embodiment, the driving member 401 is fixedly connected to the right first piston rod 303.

[0048] The working process is as follows: When the filter screen 2 on the left filters the solution, the driving fan 203 rotates clockwise (from top to bottom) under the drive of the adjacent solution. The driving fan 203 drives two flow pusher members 202 to rotate clockwise (from top to bottom) through a belt pulley. When the left flow pusher member 202 drives the solution to rotate, the solution drives the bacteria in the left filter screen 2 to rotate clockwise together. When the bacteria pass between the left collection filter screen 301 and the adjacent collection shell 3, they are intercepted by the left collection filter screen 301 and are forced to stay between the collection filter screen 301 and the adjacent collection shell 3. At this time, when the solution in the left upper connecting pipe 103 is flowing, it simultaneously enters the first cavity of the driving cylinder 4. Because there is no liquid in the right upper connecting pipe 103 in the initial state, the second cavity of the driving cylinder 4 is filled with air pressure. The hydraulic pressure in the first cavity of the driving cylinder 4 is greater than the air pressure in the second cavity of the driving cylinder 4. The driving member 401 moves to the lowest side of the driving cylinder 4 under the action of the hydraulic pressure, exhausting all the gas in the second cavity of the driving cylinder 4. During the movement of the driving member 401, it drives the first piston rod 303 on the right to move to the lowest side of the right liquid storage barrel 302. When the first piston rod 303 on the right moves, it drives the first piston rod 303 on the left to move to the uppermost side of the left liquid storage barrel 302 through the transmission gear on the fixing frame 5.

[0049] During the process of the solution entering the left filter cylinder 104 from the left upper connecting pipe 103, it simultaneously enters the upper cavity of the left liquid storage barrel 302 from the left collection shell 3. At this time, the amount of solution entering the upper cavity of the liquid storage barrel 302 is small. After the solution passes through the left filter screen 2 and moves downward to the left lower connecting pipe 105, the filtered sterile solution simultaneously enters the lower cavity of the left liquid storage barrel 302. After the solution enters the lower cavity of the liquid storage barrel 302, the gas in the lower cavity of the liquid storage barrel 302 is discharged outward through the adjacent exhaust valve.

[0050] When the filter screen 2 in the left filter cartridge 104 becomes blocked, the control terminal first pauses the liquid supply to the filter cartridge 104. The liquid pressure at the input pipe 101 decreases, and part of the liquid in the filter cartridge 104 flows back into the input pipe 101 under the action of the pressure, reducing the liquid pressure in the left filter cartridge 104 and preventing the liquid pressure in the left filter cartridge 104 from being too high, which may cause damage to the left filter screen 2 under the high liquid pressure. Subsequently, the control terminal injects hydraulic oil into the left first hydraulic telescopic rod 306. The telescopic end of the first hydraulic telescopic rod 306 extends downward, and the telescopic end of the first hydraulic telescopic rod 306 drives the moving member 305 to move downward. The moving member 305 drives the filter housing 304 to rotate through the arc-shaped groove thereon. The filter housing 304 rotates to the lower side of the connection between the left liquid storage barrel 302 and the adjacent collection housing 3. The filter housing 304 is ready to intercept the bacteria that enter the left liquid storage barrel 302 together with the liquid. Subsequently, the control terminal controls the first control valve 102 and the second control valve 106 to work, changing the flow path of the solution, so that the filter screen 2 on the right filters the solution. At this time, the solution enters the right filter cartridge 104 and the right liquid storage barrel 302 according to the same steps as when entering the left filter cartridge 104 and the left liquid storage barrel 302 above. The solution at the right upper connection pipe 103 simultaneously flows into the second cavity of the driving cylinder 4.

[0051] When the solution surges into the second cavity of the driving cylinder 4, since the second cavity of the driving cylinder 4 is communicated with the input pipe 101 through the upper right connecting pipe 103 on the right side, and the control terminal supplies liquid to the input pipe 101 again, the solution pressure in the upper right connecting pipe 103 on the right side is greater than that in the upper right connecting pipe 103 on the left side. Therefore, the liquid pressure in the second cavity of the driving cylinder 4 is greater than that in the first cavity of the driving cylinder 4. The driving member 401 moves upward under the push of the liquid pressure in the second cavity of the driving cylinder 4. During the movement of the driving member 401, the first piston rod 303 on the right side is driven to move upward together. During the upward movement of the first piston rod 303 on the right side, the first piston rod 303 on the left side is driven to move downward through the transmission gear on the fixing frame 5. During the downward movement of the first piston rod 303 on the left side, the solution in the lower cavity of the left liquid storage barrel 302 is extruded into the left lower connecting pipe 105. At this time, since the left lower connecting pipe 105 is disconnected from the second control valve 106, all the solution can only pass upward through the left filter screen 2, pass through the left collecting shell 3, and flow back to the upper cavity of the liquid storage barrel 302. When the solution passes through the left filter screen 2, it backwashes the left filter screen 2 and flushes the bacteria stuck on its mesh holes, so that the bacteria flow into the left liquid storage barrel 302 along with the solution through the left collecting shell 3. The bacteria between the left collecting shell 3 and the left collecting filter screen 301 flow into the left liquid storage barrel 302 along with the solution. And during this process, the left pushing member 202 rotates all the time, assisting the solution to rotate and flow on the filter screen 2, increasing the force for the solution to drive the bacteria to flow. When the bacteria enter the upper cavity of the left liquid storage barrel 302, the bacteria are intercepted by the left filter shell 304. When the first piston rod 303 on the left side moves to the lowermost side, the solution in the left filter cylinder 104 stops flowing.

[0052] When the right filter screen 2 is blocked, the control terminal switches the solution flow path back to the left filter cylinder 104 according to the same process as above. At this time, while injecting hydraulic oil into the right first hydraulic telescopic rod 306, the control terminal extracts the hydraulic oil in the left first hydraulic telescopic rod 306. The telescopic end of the left first hydraulic telescopic rod 306 retracts upward to reset. The telescopic end of the left first hydraulic telescopic rod 306 drives the adjacent moving member 305 to move upward. The moving member 305 drives the adjacent filter shell 304 and the bacteria therein to rotate synchronously through the arc-shaped groove on it. The left filter shell 304 returns to its initial position, and the left filter shell 304 no longer intercepts the connection between the left liquid storage barrel 302 and the adjacent collecting shell 3. At this time, during the upward movement and reset of the first piston rod 303 on the left side, the solution in the upper cavity of the liquid storage barrel 302 is all sterile solution. And during the above process, most of the bacteria stuck on the mesh holes of the left filter screen 2 are collected into the left filter shell 304. Therefore, the left filter screen 2 is no longer blocked.

[0053] When the staff has filtered all the solutions, the remaining solution in the liquid storage barrel 302 is discharged through the drain valve at the liquid storage barrel 302, and then the staff cleans the filter cartridge 104. The staff also takes out the two filter shells 304 and performs corresponding cleaning and disinfection.

[0054] Further, see Figures 3-10 , also includes: a pressure regulating component, which is arranged on the fixed frame 5, and is used to reduce the pressure in the upper connecting pipe 103 that needs recoil. The pressure regulating component includes: a negative pressure cylinder 501, which includes two, both of which are fixed to the fixed frame 5. A second piston rod 502 is slidably connected in the negative pressure cylinder 501. The negative pressure cylinder 501 is connected to the adjacent upper connecting pipe 103. The valve stem 1021 of the first control valve 102 is fixed to two fixing parts 503, and the fixing part 503 is slidably connected to the adjacent second piston rod 502.

[0055] Further, see Figure 9 , the volume of the negative pressure cylinder 501 is greater than the volume of the driving cylinder 4.

[0056] Further, see Figure 6 , Figure 8 and Figure 10 , and also includes: a second hydraulic telescopic rod 504, having two, respectively fixed to the adjacent negative pressure cylinder 501, a spring is installed between the telescopic end of the second hydraulic telescopic rod 504 and its fixed part, the second piston rod 502 is used to squeeze the telescopic end of the adjacent second hydraulic telescopic rod 504, and the first hydraulic telescopic rod 306 is connected to the adjacent second hydraulic telescopic rod 504.

[0057] In this embodiment, the first control valve 102 is of an existing structure. The first control valve 102 is composed of a valve stem 1021, a valve housing, and an electric control component. The electric control component drives the valve stem 1021 to rotate, so that the first control valve 102 communicates with different upper connecting pipes 103. The negative pressure cylinder 501 is used to extract the liquid in the upper connecting pipe 103 in the unused state, so as to further reduce the pressure at the upper connecting pipe 103, increase the pressure difference between the first cavity and the second cavity on the driving cylinder 4, so as to ensure that the driving member 401 can move to the limit position each time (move to the uppermost side or the lowermost side of the stroke), thereby enhancing the force when the solution flushes the adjacent filter screen 2 and improving the cleaning effect when flushing the filter screen 2. The volume of the negative pressure cylinder 501 is larger than the volume of the driving cylinder 4, so as to ensure that the range of the pressure in the adjacent filter cylinder 104 adjusted by the negative pressure cylinder 501 is larger than the range of the pressure change in the filter cylinder 104 when the solution in the driving cylinder 4 is injected into the filter cylinder 104, and ensure that the negative pressure created by the negative pressure cylinder 501 effectively increases the pressure difference between the first cavity and the second cavity in the driving cylinder 4. In this embodiment, the second hydraulic telescopic rod 504 replaces the hydraulic system in the above embodiment and communicates with the adjacent first hydraulic telescopic rod 306. Therefore, in this embodiment, the first hydraulic telescopic rod 306 is controlled by the adjacent second hydraulic telescopic rod 504 and is no longer controlled by the hydraulic system.

[0058] The working process is as follows: When the control terminal introduces the solution into the left filter cylinder 104, the left fixing member 503 rotates to the lower side driven by the valve stem 1021. The left fixing member 503 drives the adjacent second piston rod 502 to move to the lower side. At this time, there is no solution in the left negative pressure cylinder 501. The right fixing member 503 rotates to the upper side driven by the valve stem 1021. The right fixing member 503 drives the adjacent second piston rod 502 to move to the upper side. At this time, the right negative pressure cylinder 501 is filled with gas. When the second piston rod 502 moves to the upper side, it squeezes the telescopic end of the right second hydraulic telescopic rod 504 to retract upward. The spring on the right second hydraulic telescopic rod 504 compresses and stores energy. The hydraulic oil in the right second hydraulic telescopic rod 504 enters the adjacent first hydraulic telescopic rod 306. The telescopic end of the right first hydraulic telescopic rod 306 extends downward. At this time, the right filter housing 304 rotates to the interface between the right liquid storage barrel 302 and the right filter cylinder 104 driven by the adjacent first hydraulic telescopic rod 306 and the adjacent moving member 305.

[0059] When the left filter screen 2 is blocked and the control terminal changes the flow direction of the solution, the control terminal controls the rotation of the valve stem 1021 of the first control valve 102. The valve stem 1021 synchronously drives the two fixing members 503 thereon to rotate. The left fixing member 503 drives the adjacent one to move upward, and the right fixing member 503 moves downward. During the upward movement of the left fixing member 503, it drives the adjacent second piston rod 502 to move to the uppermost side. The left negative pressure cylinder 501 extracts the solution in the adjacent filter cylinder 104, further reducing the liquid pressure in the left filter cylinder 104, and further increasing the liquid pressure difference between the left and right filter cylinders 104, thereby ensuring that the driving member 401 moves to the limit state under the pressure difference between the first cavity and the second cavity.

[0060] When the valve stem 1021 drives the two fixing members 503 thereon to rotate, the left fixing member 503 simultaneously squeezes the hydraulic oil in the left second hydraulic telescopic rod 504 into the left first hydraulic telescopic rod 306, causing the left filter housing 304 to rotate to the interface between the right liquid storage barrel 302 and the right filter cylinder 104. The right fixing member 503 drives the second piston rod 502 to move downward, and the telescopic end of the right second hydraulic telescopic rod 504 moves downward and resets under the action of the spring thereon. The left first hydraulic telescopic rod 306 drives the adjacent filter housing 304 to move and reset. Through the above process, during the rotation of the valve stem 1021 in the first control valve 102, the valve stem 1021 drives the two first hydraulic telescopic rods 306 to complete the work in the above embodiment, eliminating the need for an additional hydraulic system and enhancing the applicable range of the device.

[0061] Embodiment 2: On the basis of Embodiment 1, referring to Figures 1-10 , a sterilization filtration method for preparing a sterile culture medium, which is applied to a sterilization filtration device for preparing a sterile culture medium, and the specific method is as follows:

[0062] S1: Control the first control valve 102 and the second control valve 106 to switch their working states so that they are connected to the same filter cylinder 104.

[0063] S2: Introduce the solution into the input pipe 101. After the solution passes through the filter screen 2 in the filter cylinder 104 in use, it is discharged outward. The solution simultaneously fills the adjacent liquid storage barrel 302 and the driving cylinder 4. The flow pusher 202 drives the surrounding solution and bacteria to rotate, and the collection filter screen 301 collects the bacteria in the adjacent filter screen 2.

[0064] S3: The driving member 401 moves under the pressure difference between the two filter cylinders 104, causing the first piston rod 303 in the working state to move upward, and the other first piston rod 303 to move downward. At the same time, the valve stem 1021 drives the two fixing members 503 to rotate, causing the second piston rod 502 in the working state to move downward, and the other second piston rod 502 to move upward.

[0065] S4: After the filter screen 2 in the working state becomes blocked, control the first control valve 102 and the second control valve 106 to switch their working states, so that the solution enters another filter cartridge 104 for filtration;

[0066] S5: After switching the state of the first control valve 102, the two second piston rods 502 move towards each other, and the two filter housings 304 rotate respectively driven by the adjacent second piston rods 502. The two first piston rods 303 move towards each other to backwash the blocked filter screen 2, and the filter housing 304 on the backwashing side intercepts the bacteria shed from the blocked filter screen 2;

[0067] S6: After the solution filtration is completed, clean the filter cartridge 104, the liquid storage barrel 302 and the filter housing 304.

[0068] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the purpose of the present invention.

Claims

1. A sterilization filtration device for preparing a sterile culture medium, characterized in that, It includes: A housing (1), the housing (1) is fixedly connected with an input pipe (101), the input pipe (101) is fixedly connected and communicated with a first control valve (102), the first control valve (102) is fixedly connected and communicated with two upper connecting pipes (103), the upper connecting pipes (103) are fixedly connected and communicated with a filter cartridge (104), the filter cartridge (104) is fixedly connected and communicated with a lower connecting pipe (105), the two lower connecting pipes (105) are jointly fixedly connected and communicated with a second control valve (106), and the second control valve (106) is fixedly connected and communicated with an output pipe (107); Filter meshes (2), there are two of them, and they correspond to the two upper connecting pipes (103) one by one, are respectively installed on the adjacent upper connecting pipes (103), and are located inside the adjacent filter cartridges (104). A flow-pushing member (202) is rotatably connected to the upper connecting pipe (103), and the flow-pushing member (202) is used to push the liquid and bacteria at the inner wall of the filter mesh (2) to rotate slowly; A driving impeller (203) is rotatably connected to the input pipe (101), and a speed measurement module for detecting its own speed is installed thereon. A belt pulley belt drive is provided between each of the two flow-pushing members (202) and the driving impeller (203); A collection mechanism is arranged inside the housing (1) and is used to collect bacteria in the filter mesh (2); The collection mechanism includes: Collection shells (3), there are two of them, and they correspond to the two filter cartridges (104) one by one, are respectively installed on the adjacent filter cartridges (104), the collection shells (3) are fixedly connected to the filter meshes (2), and the collection shells (3) are fixedly connected with collection filter meshes (301); Backwashing components, there are two of them, and they correspond to the two filter cartridges (104) one by one, and are both arranged inside the housing (1) and are used to backwash the filter mesh (2) when the adjacent filter mesh (2) is blocked; The backwashing components include: A liquid storage bucket (302), the liquid storage bucket (302) is fixedly connected inside the housing (1); A first piston rod (303) is slidably connected to the adjacent liquid storage bucket (302). The first piston rod (303) divides the inside of the liquid storage bucket (302) into an upper cavity and a lower cavity. The upper cavity of the liquid storage bucket (302) is communicated with the adjacent collection shell (3), and the lower cavity of the liquid storage bucket (302) is communicated with the adjacent lower connecting pipe (105); An interception assembly is arranged inside the upper cavity of the adjacent liquid storage bucket (302) and is used to intercept and collect bacteria entering the adjacent liquid storage bucket (302).

2. The sterilization and filtration device for preparing a sterile culture medium according to claim 1, wherein, The flow-pushing member (202) is composed of a cylinder and vertically arranged plates distributed circumferentially.

3. The sterilization filtration device for preparing a sterile culture medium according to claim 1, wherein, The interception assembly includes: A filter shell (304) is rotatably connected inside the adjacent liquid storage bucket (302); A moving member (305) is slidably connected to the adjacent first piston rod (303), and the moving member (305) is used to drive the adjacent filter shell (304) to rotate; The first hydraulic telescopic rod (306) is fixedly connected inside the adjacent liquid storage barrel (302), and the telescopic end of the first hydraulic telescopic rod (306) is fixedly connected to the adjacent moving part (305).

4. A sterilization filtration device for preparing a sterile culture medium according to claim 3, characterized in that, It further includes: A driving component, arranged inside the housing (1), for sequentially driving the two first piston rods (303) to work. The driving component includes: A driving cylinder (4), fixedly connected inside the housing (1); A driving part (401), slidably connected inside the driving cylinder (4). The driving part (401) divides the inside of the driving cylinder (4) into a first cavity and a second cavity. The first cavity inside the driving cylinder (4) is communicated with one of the upper connecting pipes (103), and the second cavity inside the driving cylinder (4) is communicated with the other upper connecting pipe (103); A fixing frame (5), fixedly connected to the two liquid storage barrels (302). A transmission gear is installed on the fixing frame (5). Both of the first piston rods (303) are meshed with the transmission gear on the fixing frame (5) through racks, and the driving part (401) is fixedly connected to one of the first piston rods (303).

5. The sterile medium preparation sterilization filtration device according to claim 4, characterized in that, It further includes: A pressure regulating component, arranged on the fixing frame (5), for reducing the pressure inside the upper connecting pipe (103) that needs to be backflushed. The pressure regulating component includes: Negative pressure cylinders (501), including two, both fixedly connected to the fixing frame (5). A second piston rod (502) is slidably connected inside the negative pressure cylinder (501). The negative pressure cylinder (501) is communicated with the adjacent upper connecting pipe (103). Two fixing parts (503) are fixedly connected to the valve rod (1021) of the first control valve (102), and the fixing parts (503) are slidably connected to the adjacent second piston rod (502).

6. The sterile filtration device for preparing a sterile culture medium according to claim 5, characterized in that, The volume of the negative pressure cylinder (501) is larger than the volume of the driving cylinder (4).

7. The sterile filtration device for preparing a sterile culture medium according to claim 6, wherein, It further includes: Second hydraulic telescopic rods (504), having two, respectively fixedly connected to the adjacent negative pressure cylinders (501). A spring is installed between the telescopic end of the second hydraulic telescopic rod (504) and its fixing part. The second piston rod (502) is used to squeeze the telescopic end of the adjacent second hydraulic telescopic rod (504). The first hydraulic telescopic rod (306) is communicated with the adjacent second hydraulic telescopic rod (504).

8. A sterilization filtration method for preparing a sterile culture medium, applied to a sterilization filtration device for preparing a sterile culture medium according to claim 7, characterized in that, The specific method is as follows: S1: Control the first control valve (102) and the second control valve (106) to switch their working states so that they are communicated with the same filter cartridge (104); S2: Pass a solution into the input pipe (101). After the solution passes through the filter mesh (2) inside the filter cartridge (104) in use, it is discharged outward. The solution simultaneously fills the adjacent liquid storage barrel (302) and the driving cylinder (4). The flow pusher (202) drives the surrounding solution and bacteria to rotate, and the collection filter mesh (301) collects the bacteria inside the adjacent filter mesh (2); S3: The driving member (401) moves under the pressure difference between the two filter cartridges (104), causing the first piston rod (303) in the working state to move upward and the other first piston rod (303) to move downward. At the same time, the valve stem (1021) drives the two fixing members (503) to rotate, causing the second piston rod (502) in the working state to move downward and the other second piston rod (502) to move upward; S4: When the filter screen (2) in the working state becomes blocked, the first control valve (102) and the second control valve (106) are controlled to switch their working states, so that the solution enters another filter cartridge (104) for filtration; S5: After switching the state of the first control valve (102), the two second piston rods (502) move towards each other, and the two filter housings (304) rotate respectively driven by the adjacent second piston rods (502). The two first piston rods (303) move towards each other to backwash the blocked filter screen (2), and the filter housing (304) on the backwashing side intercepts the bacteria shed from the blocked filter screen (2); S6: After the solution filtration is completed, the filter cartridge (104), the liquid storage bucket (302) and the filter housing (304) are cleaned.

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